Combined cell sterile operation workbench

Abstract

The utility model discloses a combined type cell sterile operation workbench. The combined type cell sterile operation workbench comprises a supporting table and a placing table, the supporting table comprises a bottom plate, the bottom plate is connected with a vertical plate, a baffle and a connecting column, the vertical plate is located between the baffle and the connecting column, and two supporting grooves are formed in the end, away from the bottom plate, of the vertical plate; the placing table comprises a body, the body is provided with a plurality of placing grooves and a plurality of liquid storage pipe inserting grooves, one end of the body is connected with a connecting piece, and the connecting piece is used for being connected with the connecting column. By arranging the supporting table, two bottle bodies can be supported at the same time, and the bottle bodies are obliquely placed, so that on one hand, the bottle openings are located in a safety area, and on the other hand, a hand taking a pipette cannot be located right above an alcohol lamp.

Description

Technical Field

[0001] The utility model belongs to the field of cell culture, and in particular relates to a combined cell aseptic operation workbench. Background Art

[0002] Cell culture is a crucial component of microbiology, immunology, and cell biology laboratories. Related operations include liquid preparation, fluid exchange, passaging, cryopreservation, and resuscitation. Tools used include sterile, empty, pre-packaged liquid bottles with capacities ranging from 100 mL to 500 mL; sterile, empty, pre-packaged liquid tubes or pre-divided tubes with capacities ranging from 1.5 mL to 5 mL; cell culture flasks ranging from 25 cm² to 75 cm²; cell culture plates; sterile centrifuge tubes, typically 50 mL; and cryopreservation tubes, typically 2 mL. Cell manipulation is highly susceptible to contamination by microorganisms such as bacteria, fungi, and mycoplasmas. Therefore, aseptic procedures must be performed within a clean bench, and the risk of contamination must be minimized. Placing an alcohol lamp within the clean bench can create a safe, sterile environment. Typically, a hemisphere with a 10 cm radius centered on the alcohol lamp flame is considered the absolute safe zone, while a 20 cm radius is considered the relative safe zone. Contamination can be effectively avoided by confining operations within this zone. Another principle of clean bench operations is a neat layout with rational planning and logistics, which can also reduce the risk of contamination. However, there is no dedicated operating platform, which brings several problems:

[0003] Question 1: The mouth of the large-capacity liquid bottle is higher than the flame of the alcohol lamp and is outside the safe zone. See attached. Figure 1 :

[0004] Question 2: The bottle mouth is vertically upward, which poses a high risk of contamination. The right hand is in the operating position above the flame, which can easily injure the wrist. To avoid wrist injury, the left hand has to be used, resulting in poor operational flexibility. For example, the left hand must be freed up to normally replace the pipette, and it is unable to hold the liquid bottle. The left hand is occupied and cannot handle unexpected situations such as liquid dripping, ear bulb falling off, and the pipette needs to be replaced due to accidental contact. Or a second operator is required, which increases manpower. See attached. Figure 2

[0005] Problem 3: Opened bottle caps or tube caps are not properly placed and securely positioned.

[0006] 1) When the bottle cap is placed upward, it is difficult to completely avoid contamination by sedimentation bacteria, and there is a risk of contamination by liquid dripping.

[0007] 2) The bottle cap is facing downward and in contact with the table, especially the bottle cap of the cell bottle, which has a certain degree of humidity. There is a risk of confusion between cells during cell passaging and the risk of microbial contamination.

[0008] 3) When the bottle cap is placed on its side, the risk of microbial contamination is the lowest, but it is easy to roll and come into high-risk contact with other items on the countertop; more than two bottle caps are prone to contact due to rolling, and when two bottle caps with the same appearance are rolled, they are easily confused, resulting in cell-to-cell or liquid-to-liquid contamination.

[0009] At present, there is no dedicated bottle cap placement device, and the bottle caps are placed randomly and chaotically during operation on the table.

[0010] Problem 4: 1mL-50mL containers lack dedicated holders in the clean bench. Non-dedicated holders occupy too much space, resulting in redundant functional areas, increasing operational difficulty and increasing the risk of contamination. Figure 3 , attached Figure 4 .

[0011] Question 5: There is no dedicated cell manipulation bench, the work layout inside the clean bench is chaotic, there is no fixed location for items, and items are placed unreasonably, increasing the risk of contamination.

[0012] Question 6: There is no dedicated cell operation table, the operation area is arbitrary, and the operation area is determined by the operator's personal habits. The logistics are chaotic. Some staff members operate right above the bottle opening, which is difficult to normalize and standardize. In particular, there is a great risk of contamination when operated by inadequately trained staff. Utility Model Content

[0013] In order to solve the above problems existing in the prior art, the technical solution adopted by the present invention is:

[0014] A combined cell sterile operation workbench, comprising a support table and a placement table;

[0015] The support platform includes a bottom plate, the bottom plate is connected to a vertical plate, a baffle and a connecting column, the vertical plate is located between the baffle and the connecting column, and two supporting grooves are provided at one end of the vertical plate away from the bottom plate;

[0016] The placement table includes a main body, which is provided with a plurality of placement slots and a plurality of liquid storage tube slots. One end of the main body is connected to a connecting piece, and the connecting piece is used to be connected to a connecting column.

[0017] By setting up a support platform, two bottles can be supported at the same time, and the bottles can be placed at an angle, so that the bottle mouth is located in a safe area on the one hand, and the hand holding the pipette will not be directly above the alcohol lamp on the other hand.

[0018] By combining multiple structures on the placement table, not only space is saved, but the entire table is placed in a safe area, reducing the risk of contamination.

[0019] Furthermore, the lower side of the base plate is connected with an anti-skid pad. By arranging the anti-skid pad, the support platform is prevented from sliding during operation.

[0020] Furthermore, the connecting piece is provided with a connecting hole, and the connecting hole is sleeved on the connecting column. Since the support platform and the placement platform are sleeved through the connecting piece and the connecting column, the placement platform can be rotated to adjust the angle between the support platform and the placement platform.

[0021] Furthermore, the placement slot includes a large tube cap lateral placement slot and a small tube cap lateral placement slot, and the liquid storage tube slot includes a large liquid storage tube slot and a small liquid storage tube slot. Different placement slots can be used to place different tube caps, and the tube caps can be placed sideways to avoid tube cap contamination.

[0022] Furthermore, the body is stepped, and comprises, from bottom to top, a first portion, a second portion, and a third portion. The first portion is provided with a lateral placement slot for a large tube cover, the second portion is provided with a lateral placement slot for a small tube cover and a slot for a small liquid storage tube, and the third portion is provided with a slot for a large liquid storage tube. By providing different slots for liquid storage tubes, it is possible to accommodate different liquid storage tubes.

[0023] The beneficial effects of the utility model are:

[0024] 1) The utility model is applicable to various cell aseptic operations and has wide versatility.

[0025] 2) During cell manipulation, all opened bottles, tubes and other containers should be placed at an angle or horizontally below the flame of the alcohol lamp, in a safe area with minimal contamination risk.

[0026] 3) All opened container lids have fixed placement positions, with the side openings facing the flame of the alcohol lamp, which reduces the risk of microbial contamination.

[0027] 4) The bottle caps are placed in an orderly manner and prevented from rolling or mixing, thus preventing contamination between containers, which is especially beneficial for cell passage.

[0028] 5) The component structure is simple, mostly a combination of plane and vertical surfaces, eliminating the excessive connections, gaps, corners and other dead corners that are prone to retaining dirt in non-professional brackets, and is more in line with sterility requirements.

[0029] 6) High space utilization rate, all positions are safe and effective spaces, and can be protected by the flame of the alcohol lamp.

[0030] 7) The ineffective space of non-dedicated holders is eliminated, and different container holders are integrated into one according to the work content, becoming a holder dedicated to cell sterile operation.

[0031] 8) The logistics direction during operation is fixed and surrounds the alcohol lamp. Each bottle cap has a fixed moving path and position, and the liquid direction has a fixed moving path. Even if a drop of liquid accidentally falls on the path, it will not affect the lower part and all are fixed in a safe area.

[0032] 9) The fixed layout eliminates bad habits such as random operations.

[0033] 10) The two-person operation can be improved to a single-person operation, saving manpower.

[0034] 11) Free up the left hand when operating alone, thus improving the operating ability.

[0035] 12) It helps to train standardized operating movements and fix aseptic operating movements and awareness, so it can be used as a teaching tool for training novices. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a schematic diagram for Question 1;

[0037] Figure 2 This is a schematic diagram for Question 2;

[0038] Figure 3 It is a schematic diagram of a liquid storage tube slot in the prior art;

[0039] Figure 4 This is a schematic diagram of the use of a liquid storage tube slot in the prior art;

[0040] Figure 5 This is a schematic diagram of the use of the support platform;

[0041] Figure 6 This is a schematic diagram of the use of the utility model;

[0042] Figure 7 It is a structural diagram of the present utility model.

[0043] Figure 8 This is a schematic diagram of the structure of the utility model Figure 2 .

[0044] Figure 9 This is a schematic diagram of the structure of the utility model Figure 3 .

[0045] In the figure: 1-support table; 11-bottom plate; 12-vertical plate; 13-baffle; 14-support groove; 15-connecting column; 16-anti-slip pad; 2-placement table; 21-main body; 22-lateral placement groove for large tube cover; 23-lateral placement groove for small tube cover; 24-small liquid storage tube slot; 25-large liquid storage tube slot; 5-connecting piece; 6-alcohol lamp; 7-liquid bottle; 71-empty bottle; 72-prefilled liquid bottle; 8-safety area; 9-pipette; 101-centrifuge tube rack; 102-cryostorage tube rack. DETAILED DESCRIPTION

[0046] The present invention will be further described below with reference to the accompanying drawings and accompanying drawings.

[0047] In order to more clearly understand the above-mentioned objectives, features and advantages of the present invention, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that the embodiments of the present application and the features therein can be combined with each other without conflict.

[0048] The terms "first", "second", "third", etc. are only used for distinction and description and should not be understood as indicating or implying relative importance.

[0049] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; they may refer to mechanical connections or electrical connections; they may refer to direct connections or indirect connections through an intermediate medium; and they may refer to internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0050] The following is a detailed description of the specific embodiments of the present invention in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention and are not intended to limit the present invention.

[0051] Example 1:

[0052] A combined cell sterile operation workbench, such as Figure 5-9 As shown, it includes a support table 1 and a placement table 2;

[0053] The support platform 1 includes a bottom plate 11, the bottom plate 11 is connected to a vertical plate 12, a baffle 13 and a connecting column 15, the vertical plate 12 is located between the baffle 13 and the connecting column 15, and two supporting grooves 14 are provided at one end of the vertical plate 12 away from the bottom plate 11;

[0054] The placement table 2 includes a main body 21 , which is provided with a plurality of placement slots and a plurality of liquid storage tube slots. One end of the main body 21 is connected to a connector 5 , and the connector 5 is used to connect to the connecting column 15 .

[0055] By setting up the support platform 1, two bottles can be supported at the same time, and the bottles can be placed at an angle, so that the bottle mouth is located in the safe area 8 on the one hand, and the hand holding the pipette 9 will not be directly above the alcohol lamp 6 on the other hand.

[0056] By combining multiple structures on the placement table 2, not only space is saved, but the entire table is placed in the safety zone 8, reducing the risk of contamination.

[0057] Example 2:

[0058] On the basis of embodiment 1, an anti-slip pad 16 is connected to the lower side of the bottom plate 11 .

[0059] The anti-skid pad 16 is provided to prevent the support platform 1 from sliding during operation.

[0060] The connecting member 5 is provided with a connecting hole, and the connecting hole is sleeved on the connecting column 15 .

[0061] Since the support platform 1 and the placement platform 2 are sleeved together through the connecting piece 5 and the connecting column 15 , the placement platform 2 can be rotated to adjust the angle between the support platform 1 and the placement platform 2 .

[0062] The placement slot includes a large tube cover lateral placement slot 22 and a small tube cover lateral placement slot 23 , and the liquid storage tube insertion slot includes a large liquid storage tube insertion slot 25 and a small liquid storage tube insertion slot 24 .

[0063] Different placement slots can be used to place different tube caps, and the tube caps can be placed sideways to avoid tube cap contamination.

[0064] Example 3:

[0065] Based on Example 2, the main body 21 is stepped, and the main body 21 includes a first part, a second part and a third part from low to high. The first part is provided with a large tube cover lateral placement groove 22, the second part is provided with a small tube cover lateral placement groove 23 and a small liquid storage tube slot 24, and the third part is provided with a large liquid storage tube slot 25.

[0066] By arranging different liquid storage tube slots, the use of different liquid storage tubes can be met.

[0067] In the prior art, the mouth of the liquid bottle 7 is higher than the flame of the alcohol lamp 6, so that the bottle mouth is located outside the safe zone 8, and the existing bottle mouth is vertically upward, which has a high risk of contamination. When the right hand operates the pipette 9, it is usually above the flame of the alcohol lamp 6, which can easily burn the wrist.

[0068] In the prior art, multiple liquid storage tube slots are mostly set in one centrifuge tube rack 101, but in actual use, only a few liquid storage tube slots are required. Since the centrifuge tube rack 101 is large, some of them are redundant. At the same time, since it occupies a large area, the freezing tube rack 102 is placed away from the alcohol lamp 6, which increases the risk of contamination.

[0069] Example 4:

[0070] like Figure 5-9As shown, support platform 1 is a safe operating support frame for pre-packaged liquid bottles with capacities ranging from 100mL to 500mL and sterile empty bottles 71. It has three vertical surfaces and a bottom surface. When in use, the bottles are placed at an angle, with the neck resting on the arc-shaped support groove 14 and the bottle mouth facing the flame of the alcohol lamp 6. The bottom plate 11 has anti-skid grooves to prevent the bottles from sliding. This assembly is the core structure of cell sterile operation.

[0071] Platform 2 supports small-volume liquid containers, allowing them to be dispensed into 1.5mL and 5mL Eppendorf tubes or other reservoirs. These include pre-packaged serum, penicillin-streptomycin stock solutions, and mycoplasma removal agents. Platform 2 is axially connected to support platform 1, and its angle can be adjusted to suit the specific requirements of the clean bench. It comprises four vertical surfaces, one bottom surface, and three work surfaces at varying heights, each with slots for different functions.

[0072] Working mode of the combination of placement table 2 and support table 1:

[0073] Liquid preparation mode: When the culture medium needs to be prepared, the culture medium pre-filled bottle and the empty bottle 71 are placed at an angle on the support table 1, with the bottle mouth close to the flame of the alcohol lamp 6, and the bottle cap is placed sideways in the bottle cap placement slot of the placement table 2, with the cap opening facing the flame, and small-volume liquid tubes such as serum and penicillin-streptomycin stock solution are inserted into the corresponding sockets of the placement table 2, and the tube cap is placed sideways in the corresponding tube cap slot, with the cap opening facing the flame.

[0074] Repackaging mode: Place the liquid bottle 7 to be repacked on the support table 1, with the bottle mouth close to the flame of the alcohol lamp 6, and the bottle cap is placed sideways on the bottle cap placement slot of the placement table 2, with the cap opening facing the flame, and the sterile empty tube for repackaging is inserted into the corresponding socket of the placement table 2, and the tube cap is placed sideways in the corresponding tube cap slot, with the cap opening facing the flame.

[0075] Specific working principle:

[0076] This application is mainly used for dividing pre-filled liquid into bottles. First, connect the support table 1 and the placement table 2, then place the alcohol lamp 6 in the inner corner of the support tube and the placement table 2, place the empty bottle 71 and the pre-filled liquid bottle 72 on the two support grooves 14 of the support table 1 respectively, and place the bottle cap on the lateral placement groove 22 of the large tube cap, and then place the liquid storage tube in the corresponding liquid storage tube slot for packaging.

[0077] The present invention is not limited to the above-mentioned optional implementation methods. Anyone can derive various other forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, any technical solution that falls within the scope defined by the claims of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A combined cell sterile operation workbench, characterized by: It comprises a supporting platform (1) and a placing platform (2); The support platform (1) comprises a bottom plate (11), the bottom plate (11) is connected to a vertical plate (12), a baffle (13) and a connecting column (15), the vertical plate (12) is located between the baffle (13) and the connecting column (15), and two supporting grooves (14) are provided at one end of the vertical plate (12) away from the bottom plate (11); The placement table (2) comprises a main body (21), wherein the main body (21) is provided with a plurality of placement slots and a plurality of liquid storage tube slots, and one end of the main body (21) is connected to a connecting piece (5), and the connecting piece (5) is used to connect to a connecting column (15).

2. The combined cell sterile operation workbench according to claim 1, characterized in that: The lower side of the bottom plate (11) is connected with an anti-slip pad (16).

3. The combined cell sterile operation workbench according to claim 1, characterized in that: The connecting piece (5) is provided with a connecting hole, and the connecting hole is sleeved on the connecting column (15).

4. The combined cell sterile operation workbench according to claim 1, characterized in that: The placement slot includes a large tube cover lateral placement slot (22) and a small tube cover lateral placement slot (23), and the liquid storage tube slot includes a large liquid storage tube slot (25) and a small liquid storage tube slot (24).

5. The combined cell sterile operation workbench according to claim 4, characterized in that: The body (21) is stepped and comprises a first part, a second part and a third part from low to high. The first part is provided with a large tube cover lateral placement groove (22), the second part is provided with a small tube cover lateral placement groove (23) and a small liquid storage tube slot (24), and the third part is provided with a large liquid storage tube slot (25).

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