Multi-channel fluorescence microscope imaging device

Abstract

The utility model discloses a multichannel fluorescence microscope imaging device which comprises a rack chassis, a left side vertical plate is arranged on one side of the rack chassis, a right side vertical plate is arranged on the other side of the rack chassis, a left side upper rack is arranged on the top of the left side vertical plate, and a right side lower rack is arranged on the top of the right side vertical plate. A right upper rack is arranged at the top of the right vertical plate, an imaging darkroom is arranged between the left vertical plate and the right vertical plate, and six light filtering blocks with different wavelengths are arranged in the imaging darkroom; each light filtering block comprises an excitation light filter positioned on one side of the light filtering block, an emission light filter positioned below the light filtering block and a two-phase color mirror positioned in the middle of the light filtering block; according to the utility model, the filter wheel is controlled to rapidly switch the filter block so as to select a proper fluorescence channel, rapid, high-efficiency and high-quality imaging is finally realized, the tedious problem that an objective lens, a focal length and a filter module need to be manually adjusted in a conventional microscope is solved, and the practicability and timeliness of a multichannel fluorescence imaging system experiment are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of wide-field fluorescence microscopes, in particular to a multi-channel fluorescence microscope imaging device. Background Art

[0002] With the continuous deepening of biomedical research, fluorescence microscopy technology, as a key tool, is playing an increasingly important role in fields such as cell biology and molecular biology.

[0003] Although traditional fluorescence microscopes can meet basic fluorescence imaging needs, they have many limitations in the imaging process of multi-color labeled samples, such as slow imaging speed, severe spectral crosstalk, difficulty in long-term observation, the need for manual focusing, and manual switching of objectives and filter components.

[0004] In addition, traditional fluorescence microscopes are highly complex to operate, require high technical skills from users, and require long experimental times, which limits their application in non-professional laboratories.

[0005] Therefore, the present application proposes a multi-channel fluorescence microscopy imaging device to solve these problems. Utility Model Content

[0006] The purpose of the present invention is to provide a multi-channel fluorescence microscope imaging device to solve the problems raised in the above background technology.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solution: a multi-channel fluorescence microscope imaging device, comprising a frame chassis, a left vertical plate provided on one side of the frame chassis, a right vertical plate provided on the other side of the frame chassis, a left upper frame provided on the top of the left vertical plate, and a right upper frame provided on the top of the right vertical plate:

[0008] An imaging darkroom is provided between the left vertical plate and the right vertical plate, wherein six filter blocks with different wavelengths are provided in the imaging darkroom, each of the filter blocks comprising an excitation filter located on one side of the filter block, an emission filter located below the filter block, and a dichroic mirror located in the middle of the filter block;

[0009] A loading platform is provided between the left upper frame and the right upper frame.

[0010] A Z-axis electric slide is provided on the surface of the chassis of the frame, a Z-axis displacement platform is slidably mounted on the Z-axis electric slide, a filter wheel is provided on the Z-axis displacement platform, and the six filter blocks are evenly arranged on the surface of the filter wheel along the circumferential direction.

[0011] Among them, an X-axis electric slide is provided on one side of the Z-axis displacement platform, an X-axis slider is slidably installed on the X-axis electric slide, a Y-axis electric slide is provided on the X-axis slider, a Y-axis slider is slidably installed on the Y-axis electric slide, and the worktable is arranged on the Y-axis slider.

[0012] Wherein, a light source is provided between the left upper frame and the right upper frame via a bracket, and the light source is located directly above the loading platform.

[0013] Among them, a vertical plate is provided on one side of the Z-axis displacement platform, and an electric objective lens converter is provided on the vertical plate. The electric objective lens converter is provided with six holes, and an objective lens is installed in each of the six holes. The six objective lenses correspond to the six filter blocks respectively.

[0014] Wherein, a collimating mirror is provided on the inner side of the right vertical plate, and an LED light source reflector is threadedly mounted on the collimating mirror.

[0015] Wherein, a tube lens is provided directly below the LED light source reflector, a fixing plate is provided on the upper surface of the frame chassis, and the tube lens is fixed on the fixing plate.

[0016] Wherein, a camera reflector is provided directly below the tube mirror, and the camera reflector is fixed on a fixing plate.

[0017] Wherein, a camera is provided below the camera reflector, the camera is fixed on the frame chassis, and the camera is electrically connected to an external computer.

[0018] A filter wheel motor is provided on one side of the Z-axis displacement platform, a driving wheel is provided at the output end of the filter wheel motor, a driven wheel is provided at the axis of the filter wheel, and the driving wheel and the driven wheel are connected via a belt transmission.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. The utility model can control the movement of the stage on the plane through the cooperation of the X-axis electric slide and the Y-axis electric slide, and can adjust the position of the stage to facilitate the function of the Z-axis electric slide. The vertical movement of the stage can be adjusted to achieve a suitable focal length. By controlling the electric nosepiece, objective lenses of different magnifications can be quickly switched to select a suitable field of view magnification. By controlling the filter wheel, filter blocks can be quickly switched to select a suitable fluorescence channel, ultimately achieving fast, efficient, and high-quality imaging. This solves the cumbersome problem of the existing traditional microscope requiring manual adjustment of the objective lens, focal length, and filter module, and improves the practicality and timeliness of multi-channel fluorescence imaging system experiments.

[0021] 2. In the present invention, six filter blocks are mounted on a filter wheel. By controlling the automatic selection of the filter wheel, the filter wheel is quickly and accurately switched to the fluorescence channel corresponding to the wavelength band of its light source. The closed housing design constructs an imaging darkroom. The filter wheel uses dovetail grooves to stabilize the filter blocks, reducing optical path deviation caused by vibration, reducing interference from stray light, and improving imaging quality. The compact structure and the hollow design of the frame reduce the space and weight occupied by the device.

[0022] 3. The utility model achieves high precision and efficiency of multi-channel fluorescence imaging by optimizing the optical path. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the explosion in the first direction of the utility model;

[0024] Figure 2 This is a schematic diagram of the explosion in the second direction of the present invention;

[0025] Figure 3 This is a schematic diagram of the explosion in the third direction of the present invention;

[0026] Figure 4 This is a schematic diagram of the rear cross-sectional structure of the utility model;

[0027] Figure 5 It is a schematic diagram of the side cross-sectional structure of the utility model.

[0028] In the figure: 1. Light source; 2. Stage; 3. Objective lens; 4. Left upper frame; 5. X-axis electric slide; 6. Collimator; 7. LED light source reflector; 8. Tube lens; 9. Camera reflector; 10. Filter wheel; 11. Filter block; 12. Y-axis electric slide; 13. Motorized objective lens converter; 14. Filter wheel motor; 15. Camera; 16. Z-axis displacement platform; 17. Z-axis electric slide; 18. Left vertical plate; 19. Right upper frame; 20. Right vertical plate; 21. Rack chassis. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0030] See also Figure 1-5The utility model provides a technical solution: a multi-channel fluorescence microscope imaging device, including a frame chassis 21, a left vertical plate 18 is provided on one side of the frame chassis 21, a right vertical plate 20 is provided on the other side of the frame chassis 21, a left upper frame 4 is provided on the top of the left vertical plate 18, and a right upper frame 19 is provided on the top of the right vertical plate 20. A closed shell is provided on the outside of the left vertical plate 18, the left upper frame 4, the right vertical plate 20 and the right upper frame 19, and an imaging darkroom is formed between the left vertical plate 18 and the right vertical plate 20 through the closed shell.

[0031] The imaging darkroom is provided with six filter blocks 11 of different wavelengths. Each filter block 11 includes an excitation filter located on one side of the filter block 11, an emission filter located below the filter block 11, and a dichroic mirror located in the middle of the filter block 11. A Z-axis electric slide 17 is provided on the surface of the frame chassis 21. A Z-axis displacement platform 16 is slidably mounted on the Z-axis electric slide 17. A filter wheel 10 is provided on the Z-axis displacement platform 16. The six filter blocks 11 are evenly arranged along the circumference of the surface of the filter wheel 10. A filter wheel motor 14 is provided on one side of the Z-axis displacement platform 16. A driving wheel is provided at the output end of the filter wheel motor 14. A driven wheel is provided at the axis of the filter wheel 10. The driving wheel and the driven wheel are connected by a belt drive. The filter wheel motor 14 can drive the driving wheel to rotate, which in turn drives the driven wheel to rotate, thereby driving the filter wheel 10 to rotate, thereby achieving the purpose of controlling the switching of the filter blocks 11 on the filter wheel 10, thereby switching the filter blocks 11 of different wavelengths.

[0032] Furthermore, the Z-axis electric slide rail 17 can drive the Z-axis displacement platform 16 to slide in the vertical direction, thereby driving the filter wheel 10 to move in the vertical direction, thereby adjusting the position of the filter wheel 10 in the vertical direction.

[0033] Among them, a stage 2 is provided between the left upper frame 4 and the right upper frame 19, and an X-axis electric slide 5 is provided on one side of the Z-axis displacement platform 16. An X-axis slider is slidably installed on the X-axis electric slide 5, and a Y-axis electric slide 12 is provided on the X-axis slider. A Y-axis slider is slidably installed on the Y-axis electric slide 12. The stage 2 is set on the Y-axis slider. The Z-axis electric slide 17 can drive the Z-axis displacement platform 16 to slide in the vertical direction, thereby driving the stage 2 and the filter wheel 10 to move synchronously in the vertical direction, so that the stage 2 moves up and down. The Z-axis displacement platform 16 is used to adjust the focus position to achieve sample imaging;

[0034] The X-axis electric slide 5 can drive the X-direction slider to move in the X direction, and the Y-axis electric slide 12 can drive the Y-direction slider to move in the Y direction, thereby driving the stage 2 to be adjusted to any position on the horizontal plane. Users can achieve precise sample positioning as needed.

[0035] A light source 1 is provided between the left upper frame 4 and the right upper frame 19 via a bracket. The light source 1 is located directly above the stage 2 and irradiates downward to provide light.

[0036] Among them, a vertical plate is provided on one side of the Z-axis displacement platform 16, and an electric objective lens converter 13 is provided on the vertical plate. The electric objective lens converter 13 is provided with six holes, and an objective lens 3 is installed in each of the six holes. The six objective lenses 3 correspond to six filter blocks 11 respectively. The electric objective lens converter 13 is located between the stage 2 and the filter wheel 10. When working, the filter block 11 and objective lens 3 required to be used are on the same vertical line with the light source 1.

[0037] The electric objective lens converter 13 can be electrically driven and can switch between different objective lenses 3 .

[0038] Among them, a collimator 6 is provided on the inner side of the right vertical plate 20, and an LED light source reflector 7 is threadedly mounted on the collimator 6. The LED light source reflector 7 guides the excitation light to the sample, and the collimator 6 ensures uniform distribution of the light beam.

[0039] Among them, a tube lens 8 is provided directly below the LED light source reflector 7, and a fixing plate is provided on the upper surface of the rack chassis 21. The tube lens 8 is fixed on the fixing plate. The tube lens 8 is used to focus the excitation light onto the sample and collect the fluorescence signal emitted by the sample.

[0040] Among them, a camera reflector 9 is provided directly below the tube mirror 8. The camera reflector 9 is fixed on a fixed plate. The camera reflector 9 and the light source 1 are on the same vertical line. The camera reflector 9 reflects the fluorescent signal to the camera 15 to ensure the clarity and resolution of the image.

[0041] A camera 15 is provided below the camera reflector 9 , and the camera 15 is fixed on the frame chassis 21 . The camera 15 is electrically connected to an external computer to transmit image data in real time.

[0042] Working principle: When in use, the sample is placed on the stage 2, and the X-axis electric slide 5 drives the X-direction slider to move in the X direction, and the Y-axis electric slide 12 drives the Y-direction slider to move in the Y direction, so that the stage 2 can be adjusted to any position on the horizontal plane to achieve precise sample positioning. Then, light is emitted by the light source 1 to illuminate the stage 2, and the Z-axis electric slide 17 drives the Z-axis displacement platform 16 to slide in the vertical direction, thereby driving the stage 2 and the filter wheel 10 to move synchronously in the vertical direction, so that the stage 2 moves up and down. The Z-axis displacement platform 16 is used to adjust the focus position to achieve sample imaging. Then, the required objective lens 3 and filter block 11 are switched as needed. At the same time, the LED light source reflector 7 guides the excitation light to the sample, the collimator 6 ensures the uniform distribution of the light beam, and the excitation light is focused onto the sample through the tube lens 8. The fluorescence signal emitted by the sample is collected, and then the camera reflector 9 reflects the fluorescence signal to the camera 15 to ensure the clarity and resolution of the image. Then the camera 15 is electrically connected to the external computer to transmit the image data in real time.

[0043] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

Claims

1. A multi-channel fluorescence microscope imaging device, comprising a frame chassis (21), wherein a left vertical plate (18) is provided on one side of the frame chassis (21), a right vertical plate (20) is provided on the other side of the frame chassis (21), a left upper frame (4) is provided on the top of the left vertical plate (18), and a right upper frame (19) is provided on the top of the right vertical plate (20), characterized in that: An imaging darkroom is provided between the left vertical plate (18) and the right vertical plate (20), and six filter blocks (11) with different wavelengths are provided in the imaging darkroom, each of the filter blocks (11) comprising an excitation filter located on one side of the filter block (11), an emission filter located below the filter block (11), and a dichroic mirror located in the middle of the filter block (11); A loading platform (2) is provided between the left upper frame (4) and the right upper frame (19).

2. A multi-channel fluorescence microscope imaging device according to claim 1, characterized in that: A Z-axis electric slide rail (17) is provided on the surface of the frame chassis (21), a Z-axis displacement platform (16) is slidably mounted on the Z-axis electric slide rail (17), a filter wheel (10) is provided on the Z-axis displacement platform (16), and six filter blocks (11) are evenly arranged on the surface of the filter wheel (10) along a circumferential direction.

3. A multi-channel fluorescence microscope imaging device according to claim 2, characterized in that: An X-axis electric slide rail (5) is provided on one side of the Z-axis displacement platform (16); an X-direction slider is slidably mounted on the X-axis electric slide rail (5); a Y-axis electric slide rail (12) is provided on the X-direction slider; a Y-direction slider is slidably mounted on the Y-axis electric slide rail (12); and the loading platform (2) is arranged on the Y-direction slider.

4. The multi-channel fluorescence microscope imaging device according to claim 1, characterized in that: A light source (1) is provided between the left upper frame (4) and the right upper frame (19) via a bracket, and the light source (1) is located directly above the loading platform (2).

5. The multi-channel fluorescence microscope imaging device according to claim 2, characterized in that: A vertical plate is provided on one side of the Z-axis displacement platform (16), and an electric objective lens converter (13) is provided on the vertical plate. The electric objective lens converter (13) is provided with six holes, and an objective lens (3) is installed in each of the six holes. The six objective lenses (3) correspond to the six filter blocks (11) respectively.

6. The multi-channel fluorescence microscope imaging device according to claim 2, characterized in that: A collimator mirror (6) is provided on the inner side of the right vertical plate (20), and an LED light source reflector (7) is threadedly mounted on the collimator mirror (6).

7. The multi-channel fluorescence microscope imaging device according to claim 6, characterized in that: A tube lens (8) is provided directly below the LED light source reflector (7); a fixing plate is provided on the upper surface of the frame chassis (21); and the tube lens (8) is fixed on the fixing plate.

8. The multi-channel fluorescence microscope imaging device according to claim 7, characterized in that: A camera reflector (9) is provided directly below the tube mirror (8), and the camera reflector (9) is fixed on a fixing plate.

9. The multi-channel fluorescence microscope imaging device according to claim 8, characterized in that: A camera (15) is provided below the camera reflector (9), the camera (15) is fixed on the frame chassis (21), and the camera (15) is electrically connected to an external computer.

10. The multi-channel fluorescence microscope imaging device according to claim 2, characterized in that: A filter wheel motor (14) is provided on one side of the Z-axis displacement platform (16), a driving wheel is provided at the output end of the filter wheel motor (14), a driven wheel is provided at the axis of the filter wheel (10), and the driving wheel and the driven wheel are connected via a belt transmission.

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