Quantitative detection consumable and detection equipment

Abstract

The utility model provides a quantifiable detection consumable and detection equipment, and relates to the in vitro diagnostic technology, the quantifiable detection consumable comprises a sample tube with a cavity and a reaction tube communicated with the sample tube, the reaction tube is provided with a reaction cavity and a sealing film for sealing the reaction cavity; the piston structure is arranged in the cavity; a first limiting structure used for limiting the piston structure to the first position and a second limiting structure used for limiting the piston structure to the third position are further arranged on the inner wall of the sample tube, the piston structure pierces the sealing film when moving to the third position from the first position, and the treatment liquid quantitatively flows into the reaction cavity; the piston structure is used for puncturing the isolating membrane isolated between the cavity and the reaction cavity, so that the treatment liquid enters the reaction cavity, the step of container replacement is omitted, meanwhile, the piston structure is pushed to move by a preset stroke, it is ensured that the mixed solution quantitatively flows into the reaction cavity, and it is avoided that excessive mixed solution enters the reaction cavity, and the detection result is affected.

Description

Technical Field

[0001] The utility model relates to an in vitro diagnostic technology, in particular to a quantitative detection consumable material and a detection device. Background Art

[0002] During the processing of samples (such as sputum, urine, and blood), it is necessary to add processing liquid to the sample in a test tube for pretreatment, and use a pipette to transfer the pretreated mixed solution to another container to achieve subsequent detection processes such as nucleic acid extraction or PCR amplification.

[0003] Since personnel usually use a dropper to pipette during the transportation process, it is difficult to accurately control the amount of mixed solution, which affects the test results. Utility Model Content

[0004] The utility model provides a quantitative detection consumable and a detection device, which aims to solve the problems in the prior art that containers need to be replaced and treatment liquid cannot be added quantitatively during sample pretreatment and subsequent processes.

[0005] In order to achieve the above-mentioned purpose, the embodiment of the present utility model provides a quantifiable detection consumable material, including:

[0006] a sample tube provided with a cavity for accommodating a processing liquid;

[0007] a reaction tube, connected to the bottom of the sample tube, the reaction tube having a reaction chamber and a sealing film for sealing the reaction chamber;

[0008] a piston structure disposed in the cavity, wherein the piston structure moves from a first position to a third position;

[0009] A first limiting structure and a second limiting structure are also provided on the inner wall of the sample tube. The first limiting structure is used to limit the piston structure to the first position, and the second limiting structure is used to limit the piston structure to the third position. When the piston structure moves from the first position to the third position, it punctures the sealing film, and the processing liquid flows into the reaction chamber in a quantitative manner.

[0010] Preferably, the piston structure further has a second position on its movement path, and the second position is located between the first position and the third position;

[0011] When the piston structure is in the first position, the piston structure is engaged with the first limiting structure, and the piston structure is spaced apart from the sealing membrane; when the piston structure is in the second position, the piston structure abuts against the sealing membrane and does not penetrate the sealing membrane; when the piston structure is in the third position, the piston structure abuts against the second limiting structure, and the piston structure tube penetrates the sealing membrane. When the piston structure moves from the second position to the third position, the processing liquid flows into the reaction chamber in a quantitative manner.

[0012] Preferably, the first limiting structure is a first convex rib, and the first convex rib is circumferentially arranged on the inner wall of the sample tube;

[0013] The piston structure includes a partition plate, and the first rib is used for clamping the partition plate.

[0014] Preferably, the second limiting structure is a second rib, the extension direction of the second rib is the same as the axial direction of the sample tube, a plurality of the second ribs are arranged on the inner wall of the sample tube, and the second ribs are used to abut the partition.

[0015] Preferably, a piston surrounding tube coaxial with the sample tube is provided in the sample tube, and the piston surrounding tube divides the cavity into a first area for accommodating the sampling member and a second area for the movement of the piston structure. The first area includes a mixing portion and a quantitative portion in the axial direction, and the quantitative portion is close to the sealing membrane. A mixing hole connecting the first and second areas is provided on the piston surrounding tube, and the mixing hole is located in the mixing portion; the first position is located in the mixing portion, and the third position is located in the quantitative portion.

[0016] Preferably, the piston structure further comprises a piston rod and a spike portion, the partition is arranged on the circumference of the piston rod, the spike portion is arranged at the end of the piston rod, and the spike portion is closer to the sealing membrane than the partition.

[0017] Preferably, the sample tube is further provided with a sealing cover, which includes a cover body detachably connected to the sample tube and a pressing portion axially provided on the cover body, and the pressing portion is used to push the piston structure to move.

[0018] Preferably, a side of the cover body facing the sample tube is provided with an avoidance cavity, and when the cover body is detachably provided on the sample tube, the top end of the sample tube is located in the avoidance cavity;

[0019] An abutment portion is provided in the avoidance cavity, and a notch is provided on the partition. The abutment portion is used to push the sampling piece through the notch into the cavity below the partition.

[0020] Preferably, the reaction tube is detachably arranged below the sample tube.

[0021] Based on the same inventive concept, the present application also provides a detection device, including the aforementioned detection consumables.

[0022] The above solution of the utility model has the following beneficial effects:

[0023] In this application, a sealing membrane is provided between the sample tube and the reaction tube, and the sealing membrane is punctured by a piston structure, so that the mixed solution in the cavity flows into the reaction chamber and reacts with the detection material in the reaction chamber. This application omits the step of replacing the container, making the operation more convenient. At the same time, the piston structure can quantitatively squeeze the mixed solution into the reaction chamber during the movement process, and the upper end of the reaction chamber is sealed after the piston structure moves to the third position, preventing excessive mixed solution from entering the reaction chamber and affecting the detection results.

[0024] Other features and advantages of the present invention will be described in detail in the subsequent detailed description of the embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is an external schematic diagram of the utility model;

[0026] Figure 2 It is a cross-sectional view of the utility model;

[0027] Figure 3 yes Figure 2 Enlarged view of part A;

[0028] Figure 4 is a cross-sectional view of the sample tube;

[0029] Figure 5 is a cross-sectional view of the reaction tube;

[0030] Figure 6 It is a schematic diagram of the piston structure;

[0031] Figure 7 is a schematic diagram of the capping;

[0032] Figure 8 is a schematic diagram of the position of the piston structure and the sealing membrane when the piston structure is in the first position;

[0033] Figure 9 is a schematic diagram of the position of the piston structure and the sealing membrane when the piston structure is in the second position;

[0034] Figure 10 It is a schematic diagram of the position of the piston structure and the sealing membrane when it is in the third position.

[0035] [Description of Reference Numerals]

[0036] 100 - sample tube, 110 - cavity, 111 - first area, 112 - second area, 113 - mixing hole, 114 - piston surrounding tube, 111a - quantitative part,

[0037] 200-reaction tube, 210-reaction chamber, 220-sealing membrane, 230-third rib, 240-bottom sealing ring

[0038] 300-piston structure, 310-partition, 320-piston rod, 330-spike, 311-notch, 340-piston,

[0039] 400-first limit structure,

[0040] 500-second limit structure,

[0041] 600-sampling pieces

[0042] 700 - sealing cover, 710 - cover body, 720 - pressing portion, 730 - avoidance cavity, 740 - abutment portion, 750 - top sealing ring. DETAILED DESCRIPTION

[0043] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, a detailed description will be given below with reference to the accompanying drawings and specific embodiments.

[0044] like Figure 1-10As shown, an embodiment of the present invention provides a quantitative detection consumable, including a sample tube 100, a reaction tube 200, and a piston structure 300. The sample tube 100 is provided with a cavity 110 for accommodating a treatment liquid. The reaction tube 200 is disposed at the bottom of the sample tube 100. The reaction tube 200 has a reaction chamber 210 and a sealing membrane 220 for sealing the reaction chamber 210. The piston structure 300 is disposed in the cavity 110 and is capable of moving from a first position to a second position and a third position in sequence. A first limiting structure 400 and a second limiting structure 500 are also disposed in the sample tube 100. That is, the first limiting structure 400 and the second limiting structure 500 are located in the cavity 110. The first limiting structure 400 is used to limit the piston structure 300 to the first position, and the second limiting structure 500 is used to limit the piston structure 300 to the third position. The piston structure 300 is located at the first position and is engaged with the first limiting structure 400. At this time, the first limiting structure 400 provides resistance for the piston structure 300 to move to the second position. When the external force applied to the piston structure 300 is greater than the resistance provided by the first limiting structure 400, the piston structure 300 moves to the second position. When the piston structure 300 is in the first position, the piston structure 300 is spaced from the sealing film 220, that is, the sealing film 220 maintains a sealing effect at this time, and the mixed liquid in the cavity 110 cannot enter the reaction chamber 210. When the piston structure 300 moves to the second position, the piston structure 300 abuts against the sealing film 220, but at this time the sealing mold has not yet been penetrated by the piston structure 300, that is, the sealing film 220 is in a critical state of being pierced, and the sealing effect is still maintained at this time. When the piston structure 300 continues to move toward the third position, the sealing film 220 is punctured and damaged. When the piston structure 300 moves to the third position, it abuts against the second limiting structure 500. The second limiting structure 500 prevents the piston structure 300 from continuing to move. In the process of the piston structure 300 moving from the second position to the third position, since the movement stroke of the piston structure 300 is the same (the stroke is the distance between the first position and the third position), the cross-section of the cavity 110 is the same, and therefore the processing liquid can flow into the reaction chamber 210 in a quantitative manner.

[0045] It is understood that the detection consumables in this application can be used for nucleic acid extraction and amplification. The sample can be sampled through a sampling member 600 such as a swab, and after sampling, the sampling member 600 is placed in the cavity 110 to react with the treatment liquid.

[0046] The present application provides a first position-limiting structure 400, which is a first rib circumferentially disposed on the inner wall of the sample tube 100 and higher than the inner wall of the sample tube 100. The piston structure 300 includes a partition 310. The piston structure 300 is limited in position by the engagement between the partition 310 and the first rib. In the absence of external force, the piston structure 300 is spaced apart from the sealing membrane 220, preventing the piston structure 300 from accidentally puncturing the sealing membrane 220. When the downward external force is greater than the resistance of the first rib, the piston structure 300 moves in the second direction.

[0047] The partition 310 also has a guiding function to prevent the piston structure 300 from tilting when assembled in the sample tube 100 .

[0048] A second limiting structure 500 is also provided in the present application. The second limiting structure 500 is a second rib. The second rib is strip-shaped. The extension direction of the second rib is the same as the axial direction of the sample tube 100. Several second ribs are arranged on the inner wall of the sample tube 100 and the second ribs are located below the first ribs. The second ribs are used to abut the partition 310.

[0049] When the piston structure 300 moves to the third position, the partition plate 310 is abutted by the second rib and cannot move further, but stops at the third position. At this time, even if a greater external force is applied, the piston structure 300 cannot move further.

[0050] Preferably, in order to ensure the clamping effect of the first rib and the abutting effect of the second rib, the thickness of the first rib needs to be smaller than the thickness of the second rib. The thickness here refers to the radial distance of the first rib or the second rib in the sample tube 100.

[0051] The aforementioned cavity 110 includes a first region 111 and a second region 112, wherein the second region 112 is disposed around the outer portion of the second region 112. The first region 111 and the second region 112 are connected via a mixing hole 113. The first region 111 is used to accommodate the sampling member 600 and the pre-placed treatment liquid, and the second region 112 is used for the sliding of the piston structure 300.

[0052] Specifically, a piston tube 114 is disposed coaxially within the sample tube 100. This tube 114 divides the cavity 110 into the aforementioned first region 111 and second region 112. The first region 111 is the area surrounded by the piston tube 114, while the second region 112 is the area between the piston tube 114 and the sample tube 100. Axially, the first region 111 is divided into a mixing section and a quantitative section 111a. The quantitative section 111a is closer to the sealing membrane 220 than the mixing section. A mixing hole 113 is located on the piston tube 114 and within the mixing section. When treatment liquid is added to the cavity 110, the balancing effect of the mixing hole 113 maintains the same liquid level in the first and second regions 111 and 112. At the same time, the mixing hole 113 is arranged in the upper mixing part instead of in the lower quantitative part 111a, which can effectively prevent foreign particles attached to the sampling piece from entering the reaction chamber 210 and affecting the detection effect.

[0053] The first position is within the mixing section, and the third position is within the quantitative section 111a. As the piston 340 moves within the first region 111, it passes through the mixing section and moves toward the quantitative section 111a. As the sealing membrane 220 is punctured, the treated solution flows into the reaction chamber 210. Because the sample tube 100 has a uniform cross-section and the piston structure 300 moves over a consistent stroke, the volume of the mixed solution extruded into the reaction chamber 210 is uniform, ensuring that a fixed amount of the mixed solution flows into the reaction chamber 210.

[0054] Furthermore, in the present application, the piston structure 300 also includes a piston rod 320 and a spike portion 330. The spike portion 330 is arranged at the end of the piston rod 320, and the partition 310 is arranged on the circumference of the piston rod 320. The spike portion 330 is closer to the sealing membrane 220 than the partition 310.

[0055] Preferably, a piston 340 is further provided on the piston rod 320. The piston 340 is located between the partition plate 310 and the spike portion 330. The piston 340 is sealedly connected to the piston surrounding tube 114 and moves synchronously with the movement of the piston rod 320 within the piston surrounding tube 114. The piston 340 cooperates with the piston surrounding tube 114 to provide a guide. Furthermore, the piston 340 also seals the piston surrounding tube 114, preventing the treatment liquid in the metering portion 111a from overflowing from the upper end of the piston surrounding tube 114.

[0056] Preferably, a reinforcing rib is provided between the piston rod 320 and the partition plate 310 .

[0057] Furthermore, the top of the sample tube 100 is provided with an opening to facilitate the placement of the sampling member 600 into the first area 111 .

[0058] The present application also includes a cover 700, which includes a cover body 710 detachably arranged on the top of the sample tube 100 and a pressing portion 720 arranged on the cover body 710. The cover body 710 is used to close the opening, and the pressing portion 720 is arranged in the axial direction of the cover body 710, and the pressing portion 720 can move along the axial direction of the cover body 710 to push the piston structure 300 to move from the first position to the third position.

[0059] Specifically, the cover 710 has a central through hole, through which the pressing portion 720 is inserted. The upper end of the pressing portion 720 is higher than the cover 710, making it easier for the user to apply force to the pressing portion 720. A limiting ring is provided at the bottom of the pressing portion 720 to limit the upper position of the pressing portion 720 relative to the cover 710, preventing the pressing portion 720 from falling off the cover 710. When the pressing portion 720 is pressed by an external force, the piston rod 320 connected to the pressing portion 720 moves downward in sync.

[0060] Preferably, a top sealing ring 750 is further provided on the central through hole, and the top sealing ring 750 is used to seal the gap between the pressing portion 720 and the central through hole.

[0061] A relief cavity 730 is further provided on the side of the cover 710 facing the sample tube 100. When the cover 710 is placed on the upper end of the sample tube 100, the top end of the sample tube 100 is located within the relief cavity 730. An abutment portion 740 is also provided within the relief cavity 730, corresponding to the notch 311 provided on the partition 310. The abutment portion 740 is used to push the sampling member 600 through the notch 311 into the cavity 110 below the partition 310.

[0062] Since most of the existing sampling pieces 600 are provided with a breaking structure, the sampling piece 600 will be broken after collecting the sample, and the part stained with the sample, usually the sterile cotton part, will be put into the processing liquid. Due to some factors, the part stained with the sample cannot contact the processing liquid when it is put into the cavity 110. Therefore, in this application, an abutment portion 740 is provided in the avoidance cavity 730, and the broken sampling piece 600 is pushed into the processing liquid through the abutment portion 740.

[0063] It is understood that the abutting portion 740 is an annular structure, which can ensure an abutting effect when abutting the sampling member 600, and prevent the abutting portion 740 from being misaligned with the sampling member 600. Preferably, the height of the abutting portion 740 is greater than the height of the cover 710, ensuring that when the cover 710 is placed on the sample tube 100, the lower end of the abutting portion 740 can extend into the sample tube.

[0064] Preferably, the annular abutting portion 740 , the cover 710 and the pressing portion 720 are coaxially arranged.

[0065] Furthermore, the reaction tube 200 is detachably disposed below the sample tube 100 .

[0066] In this embodiment, a method for assembling a reaction tube 200 and a sample tube 100 is provided. The bottom end of the reaction tube 200 is recessed upward to form a mounting groove, within which a latching slot is provided. A third rib 230 is provided at the top end of the reaction tube 200, circumferentially extending along the circumference of the reaction tube 200. The third rib 230 cooperates with the latching slot to allow the reaction tube 200 to be removably attached to the mounting groove.

[0067] Preferably, a bottom sealing ring 240 is provided at the upper end of the reaction tube 200 . The bottom sealing ring 240 is provided above the third rib 230 . The bottom sealing ring 240 is used to seal the gap between the reaction tube 200 and the mounting groove.

[0068] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles described in the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A quantifiable detection consumable, characterized in that: include: A sample tube (100) is provided with a cavity (110) for accommodating a treatment liquid; A reaction tube (200) is connected to the bottom of the sample tube (100), and the reaction tube (200) has a reaction chamber (210) and a sealing film (220) for sealing the reaction chamber (210); A piston structure (300) is disposed in the cavity (110); The inner wall of the sample tube (100) is further provided with a first limiting structure (400) for limiting the piston structure (300) to the first position and a second limiting structure (500) for limiting the piston structure (300) to the third position. When the piston structure (300) moves from the first position to the third position, the sealing film (220) is punctured, and the treatment liquid quantitatively flows into the reaction chamber (210).

2. The quantifiable detection consumable according to claim 1, characterized in that: The piston structure (300) also has a second position on its movement path, and the second position is located between the first position and the third position; When the piston structure (300) is in the first position, the piston structure (300) is engaged with the first limiting structure (400), and the piston structure (300) is spaced apart from the sealing film (220); when the piston structure (300) is in the second position, the piston structure (300) abuts against the sealing film (220) and does not penetrate the sealing film (220); when the piston structure (300) is in the third position, the piston structure (300) abuts against the second limiting structure (500), and the piston structure (300) tube penetrates the sealing film (220). When the piston structure (300) moves from the second position to the third position, the treatment liquid flows into the reaction chamber (210) in a quantitative manner.

3. The quantifiable detection consumable according to claim 1, characterized in that: The first limiting structure (400) is a first convex rib, and the first convex rib is circumferentially arranged on the inner wall of the sample tube (100); The piston structure (300) includes a partition (310), and the first rib is used for clamping the partition (310).

4. The quantifiable detection consumable according to claim 3, characterized in that: The second limiting structure (500) is a second convex rib, the extension direction of the second convex rib is the same as the axial direction of the sample tube (100), a plurality of the second convex ribs are arranged on the inner wall of the sample tube (100), and the second convex ribs are used to abut the partition (310).

5. The quantifiable detection consumable according to claim 1, characterized in that: A piston surrounding tube (114) coaxial with the sample tube (100) is provided in the sample tube (100), and the piston surrounding tube (114) divides the cavity (110) into a first area (111) for accommodating the sampling member (600) and a second area (112) for allowing the piston structure (300) to move. The first area (111) includes a mixing portion and a quantitative portion (111a) in the axial direction. The quantitative portion (111a) is close to the sealing film (220). A mixing hole (113) connecting the first area (111) and the second area (112) is provided on the piston surrounding tube (114), and the mixing hole (113) is located in the mixing portion. The first position is located in the mixing portion, and the third position is located in the quantitative portion (111a).

6. The quantifiable detection consumable according to claim 3, characterized in that: The piston structure (300) further includes a piston rod (320) and a spike portion (330), wherein the partition (310) is arranged on the circumference of the piston rod (320), and the spike portion (330) is arranged at the end of the piston rod (320), and the spike portion (330) is closer to the sealing membrane (220) than the partition (310).

7. The quantifiable detection consumable according to claim 3, characterized in that: The sample tube (100) is also provided with a sealing cover (700), and the sealing cover (700) includes a cover body (710) detachably connected to the sample tube (100) and a pressing portion (720) axially arranged on the cover body (710), and the pressing portion (720) is used to push the piston structure (300) to move.

8. The quantifiable detection consumable according to claim 7, characterized in that: A side of the cover (710) facing the sample tube (100) is provided with an avoidance cavity (730); when the cover (710) is detachably provided on the sample tube (100), the top end of the sample tube (100) is located in the avoidance cavity (730); An abutment portion (740) is provided in the avoidance cavity (730), a notch (311) is provided on the partition (310), and the abutment portion (740) is used to push the sampling member (600) through the notch (311) into the cavity (110) below the partition (310).

9. The quantifiable detection consumable according to claim 1, characterized in that: The reaction tube (200) is detachably arranged below the sample tube (100).

10. A detection device, characterized in that: The invention comprises the detection consumables according to any one of claims 1 to 9.

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