Continuous monitoring device for activity of CO2 mineralizing bacteria in oil reservoir

Abstract

The utility model relates to the technical field of mineralizing bacteria equipment, in particular to an oil reservoir CO2 mineralizing bacteria activity continuous monitoring device which comprises an incubator filled with a culture medium for culturing mineralizing bacteria. The air outlet end of the air supply assembly is located on the lower side in the incubator; the carbon dioxide concentration monitoring assembly is mounted at the upper end part of the incubator; the strain concentration monitoring assembly is mounted on the side wall of the incubator and is communicated with the incubator; the control assembly is electrically connected with the air supply assembly, the carbon dioxide concentration monitoring assembly and the strain concentration monitoring assembly. The activity of mineralized bacteria can be rapidly and continuously monitored, and the monitoring efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of mineralization bacteria equipment, in particular to a device for continuously monitoring the activity of CO2 mineralization bacteria in oil reservoirs. Background Art

[0002] Mineralizing bacteria are a type of microorganism that can participate in the biomineralization process and are widely used in environmental remediation, agricultural production, industrial production, wastewater treatment and other fields.

[0003] The crude oil extraction process produces a large amount of highly mineralized wastewater, which contains large amounts of difficult-to-separate crude oil and various organic matter. To avoid direct discharge and serious environmental pollution, highly mineralized wastewater needs to be treated before being reused. During this treatment process, a large amount of reagents must be added to the water to produce precipitation, which is then filtered and dehydrated to form sludge. However, this treatment process not only increases processing costs but also increases the amount of sludge waste to be processed and the difficulty of handling it. For example, a prior application with publication number CN 221166545U discloses a sewage activated sludge microbial flora concentration monitoring device for monitoring the microbial content in sewage activated sludge from a sewage treatment plant. Mineralizing bacteria are a targeted strain. After screening and acclimation, the strain needs to be transplanted into a culture medium to maintain its activity, facilitating subsequent rapid transplantation into highly mineralized wastewater, allowing rapid adaptation to the environment and improving the efficiency of mineralized water treatment. However, in the prior art, strain activity is mainly detected by plate colony counts or flow cytometry. These methods are time-consuming and difficult to meet the demand for rapid monitoring of strain activity during continuous culture.

[0004] Therefore, those skilled in the art are committed to developing a device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs, which is conducive to quickly and continuously monitoring the activity of mineralizing bacteria and improving monitoring efficiency. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide a device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs, which is conducive to quickly and continuously monitoring the activity of mineralizing bacteria and improving the monitoring efficiency.

[0006] The technical solution of the utility model to solve the above technical problems is as follows:

[0007] A device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs, comprising a culture vessel filled with a culture medium for cultivating mineralizing bacteria;

[0008] An air supply component, wherein the air outlet of the air supply component is communicated with the lower side of the incubator, and a sterilization component is installed between the air supply component and the incubator;

[0009] A carbon dioxide concentration monitoring component is installed at the upper end of the incubator;

[0010] a strain concentration monitoring component, the strain concentration monitoring component being mounted on a side wall of the incubator and communicating with the incubator;

[0011] a control component electrically connected to the air supply component, the carbon dioxide concentration monitoring component, and the strain concentration monitoring component;

[0012] The strain concentration monitoring component includes a filter, a filter pump, a diluter and a detection component connected in sequence;

[0013] The detection component includes a detection tube, which is connected to the diluter and is a transparent tube. An ultraviolet irradiation component and a visible light irradiation component are installed on the detection tube. The detection tube is also connected to a photomultiplier tube, and the photomultiplier tube is electrically connected to the control component.

[0014] The beneficial effect of adopting the above scheme is that during the acclimation and cultivation process, the mineralized bacteria strain consumes oxygen and produces carbon dioxide. By setting up the incubator, air supply component, carbon dioxide concentration monitoring component, strain concentration monitoring component and control component, a complete monitoring system is formed. It can simultaneously monitor the carbon dioxide concentration and strain concentration during the mineralized bacteria cultivation process, providing multi-dimensional data support for accurately evaluating the activity of the mineralized bacteria.

[0015] The detection component is equipped with an ultraviolet irradiation component and a visible light irradiation component, which can emit light of specific wavelengths respectively. Combining the detection of two different wavelengths can more comprehensively and accurately evaluate the strain concentration and growth status, and improve the credibility of the monitoring results.

[0016] On the basis of the above technical solution, the present invention can also be improved as follows.

[0017] Furthermore, the air supply component includes an air pump, which is electrically connected to the control component. The output end of the air pump is connected to the dust removal component and the sterilization component in sequence. The output end of the sterilization component is connected to the lower side of the incubator through an air supply pipe. A first precision carbon dioxide concentration sensor is installed on the air supply pipe, and the first precision carbon dioxide concentration sensor is electrically connected to the control component.

[0018] The beneficial effects of adopting the above further solution are: the air pump is used to pump air, and the dust removal component and the sterilization component can effectively remove impurities and microorganisms in the air, ensuring that the air entering the incubator is pure, avoiding the interference of impurities and foreign microorganisms on the mineralization bacteria cultivation process, and ensuring the accuracy of the monitoring results;

[0019] The first precision carbon dioxide concentration sensor is installed on the air supply pipe, which can accurately detect the initial carbon dioxide concentration in the air entering the incubator, providing a comparison benchmark for subsequent accurate monitoring of changes in carbon dioxide concentration produced by the metabolism of mineralizing bacteria, and helping to more accurately analyze the activity of mineralizing bacteria.

[0020] Furthermore, the output end of the air supply pipe is also connected to an aeration device, and the aeration device is located in the culture vessel.

[0021] The beneficial effect of adopting the above-mentioned further scheme is that the aeration device is located in the incubator, which can enable the air to fully contact the culture medium and strains in the incubator, increase the dissolved oxygen content, meet the oxygen demand of the growth and metabolism of mineralizing bacteria, promote the growth and reproduction of mineralizing bacteria, and improve their metabolic activity, thereby enhancing the absorption and conversion capacity of carbon dioxide, improving the effectiveness of the entire monitoring device, and at the same time facilitating the stirring and mixing of the culture medium, so that the strains can fully contact the nutrient matrix in the culture medium.

[0022] Furthermore, the upper end of the culture vessel is also connected to a feeding pipe.

[0023] The beneficial effect of adopting the above further solution is that the feeding pipe connected to the upper end of the incubator facilitates the staff to add culture medium components and nutrients in a timely manner, improves work efficiency, and ensures the stability of the mineralization bacteria culture process.

[0024] Furthermore, the carbon dioxide concentration monitoring component includes a second precision carbon dioxide concentration sensor, which is electrically connected to the control component and is installed on the exhaust pipe.

[0025] The beneficial effect of adopting the above further scheme is: the carbon dioxide concentration monitoring component uses a second precision carbon dioxide concentration sensor installed on the exhaust pipe, which can accurately detect the carbon dioxide concentration in the exhaust gas in the incubator, directly reflecting the amount of carbon dioxide released during the metabolism of mineralizing bacteria. By comparing with the initial concentration of carbon dioxide entering the incubator, the absorption and utilization of carbon dioxide by mineralizing bacteria can be accurately calculated, and then their activity can be effectively evaluated.

[0026] Furthermore, the other end of the filter is connected to the filter pump, and the filter has multiple layers of filter membranes for filtering impurities;

[0027] The output end of the filter pump is connected to the diluter, and the diluter is connected to the detection component.

[0028] The beneficial effects of adopting the above further scheme are: in the strain concentration monitoring component, the filter has multiple layers of filter membranes, which can effectively filter out impurities in the culture medium and improve the purity of the test sample. The filter pump can transport the filtered sample to the diluter, and the diluter can dilute the sample as needed to make the detection concentration more accurate.

[0029] Furthermore, the wavelength of ultraviolet light emitted by the ultraviolet irradiation component is 260nm, and the wavelength of visible light emitted by the visible light irradiation component is 600nm.

[0030] The beneficial effects of adopting the above further scheme are: 260nm ultraviolet light can be absorbed by nucleic acids and can be used to quantitatively analyze the genetic material content of the strain and understand the proliferation of the strain; 600nm visible light is used to measure the turbidity of the strain, which is positively correlated with the biomass of the strain. Through the detection of these two specific wavelengths, the concentration and growth status of the strain can be accurately assessed, providing a more accurate basis for monitoring the activity of mineralizing bacteria.

[0031] Furthermore, the photomultiplier tube is also connected to a waste liquid collection box.

[0032] The beneficial effect of adopting the above further solution is: the photomultiplier tube is connected to the waste liquid collection box, which can timely collect the waste liquid generated during the detection process, prevent the waste liquid from being discharged at will and causing pollution to the environment, and also avoid the waste liquid from corroding or interfering with the internal components of the monitoring device. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a schematic diagram of the structure of the device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to the utility model;

[0034] Figure 2 This is a schematic diagram of the planar structure of the device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to the utility model.

[0035] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0036] 1. Incubator; 2. Air supply assembly; 3. Carbon dioxide concentration monitoring assembly; 4. Bacteria strain concentration monitoring assembly; 5. Air pump; 6. Dust removal assembly; 7. Sterilization assembly; 8. Air supply pipe; 9. First precision carbon dioxide concentration sensor; 10. Aeration device; 11. Feeding pipe; 12. Second precision carbon dioxide concentration sensor; 13. Exhaust pipe; 14. Filter; 15. Filter pump; 16. Diluter; 17. Detection tube; 18. Ultraviolet irradiation assembly; 19. Visible light irradiation assembly; 20. Photomultiplier tube; 21. Waste liquid collection box. DETAILED DESCRIPTION

[0037] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0038] In the description of the present invention, it should be understood that the terms "center", "length", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "inside", "outside", "peripheral", "circumferential" and the like indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the system or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0039] In the description of the present invention, “a plurality of” means at least two, such as two, three, etc., unless otherwise clearly defined.

[0040] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0041] like Figure 1 、 Figure 2 As shown, a device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs includes a culture vessel 1, which is filled with a culture medium for culturing mineralizing bacteria. The culture medium mainly includes yeast paste, peptone, glucose, sodium chloride, agar and distilled water, which can effectively promote the reproduction and metabolic activity of mineralizing bacteria.

[0042] A feeding pipe 11 is also connected to the upper end of the culture vessel 1. Without affecting the culture environment in the culture vessel 1, culture medium components or nutrients are added to the culture vessel 1 regularly and quantitatively to maintain the nutritional balance of the culture medium and ensure that the mineralized bacteria can obtain sufficient nutrients throughout the entire culture monitoring cycle, thereby ensuring the stability of their growth and metabolism.

[0043] Air supply component 2,

[0044] The air outlet end of the air supply component 2 is connected to the lower side of the incubator 1;

[0045] A carbon dioxide concentration monitoring component 3 is installed at the upper end of the incubator 1;

[0046] The strain concentration monitoring component 4 is installed on the side wall of the culture vessel 1 and communicates with the culture vessel 1;

[0047] The control component (not shown in the figure) is electrically connected to the air supply component 2, the carbon dioxide concentration monitoring component 3 and the strain concentration monitoring component 4.

[0048] like Figure 1 、 Figure 2 As shown, in some embodiments, the air outlet end of the air supply component 2 is located at the lower side of the incubator 1, which helps the air to form a bottom-up flow path in the incubator 1, so that the air can fully contact the culture medium and the strain, promote the dissolution and diffusion of oxygen, and meet the demand for oxygen for the growth and metabolism of mineralized bacteria. Specifically, the air supply component 2 includes an air pump 5, which is electrically connected to the control component and can adjust the air delivery flow and pressure according to the instructions of the control component to adapt to the changes in the demand for oxygen by mineralized bacteria in different culture stages. The output end of the air pump 5 is connected to the dust removal component 6 and the sterilization component 7 in sequence, which can effectively remove dust, particles and other impurities in the air, while the sterilization component 7 uses physical sterilization methods such as ultraviolet rays or high temperature to kill bacteria, fungi and other microorganisms in the air, ensuring that the air entering the incubator 1 is pure and sterile, avoiding interference from foreign microorganisms, and ensuring the accuracy of the monitoring results.

[0049] The output end of the sterilization assembly 7 is connected to the underside of the incubator 1 via an air supply pipe 8. The air supply pipe 8 is made of corrosion-resistant, low-adsorbability materials to prevent loss or contamination of gases such as carbon dioxide during transportation. A first precision carbon dioxide concentration sensor 9 is mounted on the air supply pipe 8 and is electrically connected to the control assembly. The first precision carbon dioxide concentration sensor 9 utilizes advanced optical detection technology to accurately detect the initial carbon dioxide concentration in the air entering the incubator 1 in real time. This data is then transmitted to the control assembly, providing a comparison benchmark for subsequent accurate monitoring of changes in carbon dioxide concentration resulting from the metabolism of mineralizing bacteria, facilitating more precise analysis of the activity of the mineralizing bacteria. The output end of the air supply pipe 8 is also connected to an aeration device 10, which is located in the culture vessel 1. The aeration device 10 is a porous aeration head or a microporous aeration tube, which can evenly release air into the culture medium in the form of tiny bubbles, increase the contact area between the air and the culture medium, further improve the transfer efficiency of dissolved oxygen, meet the efficient demand for oxygen for the growth and metabolism of mineralizing bacteria, promote the growth and reproduction of mineralizing bacteria, improve their metabolic activity, thereby enhancing the absorption and conversion capacity of carbon dioxide, and improving the effectiveness of the entire monitoring device. At the same time, it is beneficial to stir and mix the culture medium so that the strain can fully contact the nutrient matrix in the culture medium.

[0050] like Figure 1 、 Figure 2As shown, in another embodiment, the carbon dioxide concentration monitoring component 3 includes a second precision carbon dioxide concentration sensor 12, which is electrically connected to the control component and mounted on the exhaust pipe 13. The second precision carbon dioxide concentration sensor 12 is used to detect the concentration of carbon dioxide in the gas discharged from the exhaust pipe 13. By comparing the initial concentration of carbon dioxide entering the incubator 1 with the concentration of carbon dioxide in the exhaust gas, and combining parameters such as the gas volume and air flow rate within the incubator 1, the control component can accurately calculate the amount of carbon dioxide emitted by the oil reservoir CO2 mineralizing bacteria, thereby effectively evaluating their activity.

[0051] In the embodiment, the strain concentration monitoring component 4 includes a filter 14, a filter pump 15, a diluter 16, and a detection component connected in sequence. One end of the filter 14 is connected to the culture vessel 1, and the other end of the filter 14 is connected to the filter pump 15. The filter pump 15 can be a peristaltic pump. The filter 14 has multiple layers of filter membranes for filtering impurities. The filter membranes can effectively intercept impurity particles in the culture medium and allow strains to pass through without damaging the strains, thereby improving the purity of the test sample. The output end of the filter pump 15 is connected to the diluter 16. The diluter 16 accurately dilutes the sample according to a pre-set dilution ratio or real-time detection requirements to prevent excessive strain concentration from saturating the detection signal or excessively low concentration from affecting detection accuracy. The diluter 16 is connected to the detection component.

[0052] In one embodiment, the detection assembly includes a detection tube 17 connected to a diluter 16. Detection tube 17 is a transparent tube made of a material highly transmissive to ultraviolet and visible light to minimize light attenuation during transmission. An ultraviolet irradiation assembly 18 and a visible light irradiation assembly 19 are mounted on detection tube 17. In a specific embodiment, the ultraviolet light emitted by ultraviolet irradiation assembly 18 has a wavelength of 260 nm, and the visible light emitted by visible light irradiation assembly 19 has a wavelength of 600 nm. The ultraviolet light emitted by ultraviolet irradiation assembly 18 has a wavelength of 260 nm, which is specifically absorbed by nucleic acid molecules. When ultraviolet light irradiates a sample containing a bacterial strain, the nucleic acid molecules absorb the ultraviolet light and generate a light absorption signal. By detecting the intensity of this signal, the genetic material content of the strain can be quantitatively analyzed, thereby understanding the proliferation of the strain, as an increase in the number of strains is typically accompanied by the replication and accumulation of genetic material. The visible light emitted by visible light irradiation assembly 19 has a wavelength of 600 nm, which is highly sensitive for detecting turbidity in bacterial cells. Bacterial cells in culture medium scatter 600 nm visible light, and the intensity of the scattered light is positively correlated with the biomass of the strain. Measuring the intensity of the scattered light indirectly reflects the growth concentration and biomass of the strain. Combining the detection results of these two different wavelengths allows for a more comprehensive and accurate assessment of the strain concentration and growth status. The activity of the strain can be determined based on the strain concentration and carbon dioxide production.

[0053] Detection tube 17 is also connected in turn to a photomultiplier tube 20, which is electrically connected to the control unit. Photomultiplier tube 20 is a highly sensitive photoelectric conversion device that amplifies weak light signals and converts them into electrical signals. It has excellent response characteristics to both ultraviolet and visible light, and can accurately detect the light absorption or scattering signals generated by the strain sample in detection tube 17, converting these signals into electrical signals and transmitting them to the control unit.

[0054] The photomultiplier tube 20 is also connected to a waste liquid collection box 21, which is used to collect waste liquid generated during the detection process. It is equipped with an anti-corrosion coating and a liquid level monitoring device to prevent the waste liquid from being discharged at will and causing pollution to the environment. It also avoids the waste liquid from corroding or interfering with the internal components of the monitoring device. The liquid level monitoring device can monitor the waste liquid collection situation in real time. When the amount of waste liquid approaches the upper limit of the collection box capacity, it will send an alarm signal to the control component to prompt the staff to deal with the waste liquid in time to ensure the normal operation of the monitoring device.

[0055] In a specific embodiment, the control component can be implemented as a high-performance microprocessor or programmable logic controller (PLC), possessing powerful data processing, signal conversion, and logic control capabilities. The control component not only electrically connects the air supply component 2, the carbon dioxide concentration monitoring component 3, and the strain concentration monitoring component 4, but also coordinates the operation of each component, receives data signals transmitted by various sensors and detection equipment, and performs real-time data analysis and processing. Based on a preset algorithm model and monitoring standards, the control component can automatically determine whether the mineralizing bacteria activity meets the expected requirements and display the monitoring results in intuitive charts, curves, or digital form on a connected display screen. It also has a data storage function, saving historical monitoring data to facilitate subsequent data analysis and research. Furthermore, the control component can automatically adjust the operating parameters of the air supply component 2, such as the flow rate of the air pump 5 and the operating status of the aeration device 10, based on the monitoring results. This allows for precise control of the culture environment to maintain optimal growth and metabolic conditions for the mineralizing bacteria, ensure the stability and continuity of the monitoring process, and improve monitoring efficiency and accuracy, providing strong technical support for the research and application of the activity of CO2-mineralizing bacteria in oil reservoirs.

[0056] In other embodiments, a discharge pipe is further installed at the bottom of the incubator 1, and an electric control valve is installed on the discharge pipe for automatically discharging mineralized bacteria of appropriate concentration and activity to a designated location.

[0057] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs, characterized by: The invention comprises a culture vessel (1), wherein the culture vessel (1) is filled with a culture medium for culturing mineralizing bacteria; An air supply component (2), wherein an air outlet end of the air supply component (2) is in communication with the lower side of the incubator (1), and a sterilization component (7) is further installed between the air supply component (2) and the incubator (1); A carbon dioxide concentration monitoring component (3), the carbon dioxide concentration monitoring component (3) being installed at the upper end of the incubator (1); a strain concentration monitoring component (4), the strain concentration monitoring component (4) being mounted on a side wall of the culture vessel (1) and communicating with the culture vessel (1); A control component, the control component being electrically connected to the air supply component (2), the carbon dioxide concentration monitoring component (3), and the strain concentration monitoring component (4); The strain concentration monitoring component (4) includes a filter (14), a filter pump (15), a diluter (16) and a detection component connected in sequence; The detection assembly comprises a detection tube (17), the detection tube (17) is connected to the diluter (16), and the detection tube (17) is a transparent tube. An ultraviolet irradiation assembly (18) and a visible light irradiation assembly (19) are installed on the detection tube (17). The detection tube (17) is also connected to a photomultiplier tube (20), and the photomultiplier tube (20) is electrically connected to the control assembly.

2. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: The air supply component (2) includes an air pump (5), which is electrically connected to the control component. The output end of the air pump (5) is connected to the dust removal component (6) and the sterilization component (7) in sequence. The output end of the sterilization component (7) is connected to the lower side of the incubator (1) through an air supply pipe (8). A first precision carbon dioxide concentration sensor (9) is installed on the air supply pipe (8), and the first precision carbon dioxide concentration sensor (9) is electrically connected to the control component.

3. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 2, characterized in that: The output end of the air supply pipe (8) is also connected to an aeration device (10), and the aeration device (10) is located in the culture vessel (1).

4. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: The upper end of the culture vessel (1) is also connected to a feeding pipe (11).

5. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: The carbon dioxide concentration monitoring component (3) comprises a second precision carbon dioxide concentration sensor (12), the second precision carbon dioxide concentration sensor (12) is electrically connected to the control component, and the second precision carbon dioxide concentration sensor (12) is mounted on the exhaust pipe (13).

6. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: One end of the filter (14) is in communication with the culture vessel (1), and the other end of the filter (14) is connected to the filter pump (15). The filter (14) has multiple layers of filter membranes for filtering impurities. The output end of the filter pump (15) is connected to the diluter (16), and the diluter (16) is connected to the detection component.

7. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: The ultraviolet light emitted by the ultraviolet irradiation component (18) has a wavelength of 260 nm, and the visible light emitted by the visible light irradiation component (19) has a wavelength of 600 nm.

8. The device for continuously monitoring the activity of CO2 mineralizing bacteria in oil reservoirs according to claim 1, characterized in that: The photomultiplier tube (20) is also connected to a waste liquid collection box (21).

Images