Slide system for constructing plankton sample library

Abstract

The utility model provides a slide system for constructing a plankton sample library. The slide system comprises a glass slide, a cover glass, an objective lens, an injection pump, a sample bottle, a capillary tube, a microscopic imaging system and a controller, two groove structures are arranged on the glass slide and are used for accommodating capillary tubes through which liquid passes; the cover glass is used for covering the glass slide and forming a sample cell space; the sample bottle, the sample pool space and the injection pump are circularly communicated through a capillary tube; the injection pump is used for driving a plankton sample in the sample bottle to circularly flow through the sample pool; the microscopic imaging system captures and displays microscopic images of plankton through an objective lens; and the controller is in communication connection with the microscopic imaging system and is used for constructing a plankton sample library according to the microscopic image. According to the utility model, the experimental process can be simplified, the construction working efficiency of the plankton sample library can be obviously improved, and meanwhile, the multi-view morphological structure information of plankton can be comprehensively provided.

Description

Technical Field

[0001] The utility model relates to the technical field of plankton, in particular to a slide system for constructing a plankton sample library. Background Art

[0002] When constructing a plankton sample library, it is often necessary to observe and record the morphological characteristics of plankton from different observation angles (such as frontal view, ventral view, side view, etc.). The frontal view can show the main structural characteristics of the organism, the ventral view reveals the details of the abdominal structure and appendages of the zooplankton, and the side view can provide information on other three-dimensional features such as cell thickness and curvature. Some important morphological features can only be clearly identified at specific observation angles. Therefore, collecting images from different observation angles can obtain more comprehensive and accurate morphological and structural information of plankton.

[0003] However, current slide systems typically only provide single-view observations. To observe plankton morphology from different angles, researchers often have to prepare multiple samples or use a dissecting needle to move cells under a microscope to obtain morphological and structural information from different perspectives. This not only increases the experimental workload but also reduces the efficiency of sample library construction. Utility Model Content

[0004] The utility model provides a slide system for constructing a plankton sample library, so as to solve the problem of how to improve the efficiency of constructing the sample library.

[0005] In a first aspect, the present invention provides a slide system for constructing a plankton sample library, comprising:

[0006] slides, cover slips, objectives, syringe pumps, sample vials, capillaries, microscopy imaging systems, and controllers;

[0007] The slide is provided with two groove structures for accommodating capillaries for liquid to pass through;

[0008] The cover glass is used to cover the slide and form a sample pool space;

[0009] The sample bottle, the sample pool space and the syringe pump are connected in a capillary circulation manner; the syringe pump is used to drive the plankton sample in the sample bottle to circulate through the sample pool;

[0010] The microscopic imaging system captures and displays microscopic images of plankton through the objective lens;

[0011] The controller is in communication with the microscopic imaging system and is used to construct a plankton sample library based on the microscopic images.

[0012] In a possible implementation, the groove structure is a U-shaped hole.

[0013] In a possible implementation, the U-shaped hole includes a liquid inlet hole and a liquid outlet hole;

[0014] The diameter of the liquid inlet hole is larger than the diameter of the liquid outlet hole.

[0015] In a possible implementation, the liquid inlet is connected to the injection pump; and the liquid outlet is connected to the sample bottle.

[0016] In a possible implementation, the groove structure is a circular groove, and the outer wall of the capillary is embedded in the circular groove.

[0017] In a possible implementation, the slide system further includes a rubber sealing ring sleeved on the outer wall of the capillary tube.

[0018] In a possible implementation manner, a gel resin adhesive is filled between the capillary tube and the two groove structures.

[0019] In a possible implementation, the joint between the cover glass and the slide glass is filled with a gel resin adhesive.

[0020] In a possible implementation, the slide system further includes a three-way valve; three ports of the three-way valve are respectively connected to the injection pump, the sample bottle, and the slide.

[0021] In a possible implementation, the slide system further includes a digital camera for sampling and photographing the image in the objective lens.

[0022] The utility model provides a slide system for constructing a plankton sample library, which is composed of a slide, a cover glass, an objective lens, a syringe pump, a sample bottle, a capillary tube, a microscopic imaging system, and a controller. Without the need to replace the sample, the system uses the microscopic imaging system to capture the morphological characteristics of plankton from different perspectives through the periodic circulation of liquid in the slide sample pool. This slide system not only simplifies the experimental process and significantly improves work efficiency, but also can comprehensively provide multi-perspective morphological and structural information of plankton. This is of great value for the construction of a standardized plankton sample library, understanding the taxonomic status of plankton, and deepening the understanding of its biological functions and ecological significance. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments or descriptions of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0024] Figure 1 This is a structural diagram of a slide system for constructing a plankton sample library provided by an embodiment of the present invention;

[0025] Figure 2 This is a schematic diagram of the connection between a glass slide, a cover glass, and a capillary provided in one embodiment of the present invention;

[0026] Figure 3 It is a structural schematic diagram of a slide system for constructing a plankton sample library provided by another embodiment of the present invention. DETAILED DESCRIPTION

[0027] To help those skilled in the art better understand this solution, the following will clearly describe the technical solutions in the embodiments of this solution in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of this solution, not all of it. Based on the embodiments of this solution, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of this solution.

[0028] Throughout the specification, claims, and accompanying figures of this solution, the term "including" and any variations thereof mean "including, but not limited to," and are intended to cover non-exclusive inclusions and are not limited to the examples listed herein. Furthermore, the terms "first" and "second," etc., are used to distinguish between different objects, not to describe a specific order.

[0029] The following is a detailed description of the implementation of the present invention with reference to the accompanying drawings:

[0030] Figure 1 This is a schematic diagram of the structure of a slide system for constructing a plankton sample library provided by an embodiment of the present invention. Figure 1 The slide system for constructing a plankton sample library includes: a slide 1, a cover glass 2, an objective lens 3, a syringe pump 4, a sample bottle 5, a capillary 6, a microscopic imaging system 7 and a controller 8.

[0031] like Figure 2 As shown, the slide glass 1 is provided with two groove structures 102 for accommodating the capillary tubes 6 for liquid to pass through. The cover glass 2 is used to cover the slide glass 1 and form a sample pool space 101.

[0032] The sample pool space ensures that plankton can be observed from different viewing angles (such as front view, ventral view, side view, etc.), so as to realize the collection of information on the main structural characteristics of the organisms displayed by the front view, the details of the abdominal structure and appendages of the zooplankton displayed by the ventral view, the cell thickness, degree of curvature displayed by the side view, and other three-dimensional characteristics, so as to obtain the morphological and structural information of plankton more comprehensively and accurately.

[0033] The sample bottle 5 , the sample pool space 101 and the syringe pump 4 are circulatoryly connected via the capillary 6 , and the syringe pump 4 is used to drive the plankton sample in the sample bottle 5 to circulate through the sample pool.

[0034] The sample bottle 5 is used to hold the plankton sample and serves as the starting and ending point of the liquid circulation. The syringe pump 4 is used to control the flow and stillness of the liquid to ensure that the sample can be periodically updated during the collection interval.

[0035] The precise control function of the syringe pump 4 is used to precisely control the amount of liquid introduced. The sample bottle 5, the sample pool space 101 and the syringe pump 4 are circulated and connected via the capillary 6 to ensure the continuity and stability of the liquid flow.

[0036] The microscopic imaging system 7 captures and displays microscopic images of plankton through the objective lens 3 .

[0037] The controller 8 is in communication with the microscopic imaging system 7 and is used to construct a plankton sample library based on the microscopic images.

[0038] The system can automatically and efficiently collect the morphological characteristics of plankton samples from different perspectives, significantly improving the efficiency of sample library construction and simplifying the experimental process.

[0039] In practice, microscopic imaging system 7 continuously captures the morphological structure of plankton under objective lens 3. Between image captures, syringe pump 4 circulates the plankton sample in sample bottle 5 through the sample pool, automatically rotating the sample batch. After the sample rotation is complete, the system re-enters the capture cycle, ensuring continuous and automated data acquisition.

[0040] The slide system provided in the embodiment of the present application can complete the detection of plankton samples in natural water bodies such as lakes, rivers, and seawater, and has a wide range of uses.

[0041] In this embodiment, the slide system used to construct a plankton sample library consists of a slide 1, a cover glass 2, an objective lens 3, a syringe pump 4, a sample bottle 5, a capillary tube 6, a microscopic imaging system 7, and a controller 8. This system, without the need for sample replacement, uses the microscopic imaging system 7 to capture the morphological characteristics of plankton from different perspectives by periodically circulating the liquid in the sample reservoir of the slide 1. This slide system not only simplifies the experimental process and significantly improves work efficiency, but also provides comprehensive multi-view morphological and structural information on plankton. This is of great value for the construction of standardized plankton sample libraries, understanding the taxonomic status of plankton, and deepening understanding of their biological functions and ecological significance.

[0042] In different embodiments, the form of the groove structure 102 is different.

[0043] In a possible implementation, the groove structure 102 is a U-shaped hole.

[0044] In this embodiment, the groove structure 102 can effectively accommodate and fix the capillary 6, thereby improving the stability and functionality of the overall structure, ensuring that the plankton sample can stably enter and exit the sample pool space 101, and facilitating the observation of the diverse morphologies of the plankton.

[0045] In other possible implementations, the groove structure 102 is a circular groove, and the outer wall of the capillary tube 6 is embedded in the circular groove.

[0046] In this embodiment, the groove structure 102 is designed as a circular groove, and an embedded connection is achieved between the outer wall of the capillary tube 6 and the circular groove. This connection method not only ensures the stable fixation of the capillary tube 6 and maintains good sealing performance during use, but also effectively prevents liquid leakage.

[0047] Based on the aforementioned different groove structures 102, in order to improve the liquid replacement efficiency, it is necessary to optimize and improve the relevant structures. Specifically, the following possible implementation methods can be adopted:

[0048] When the groove structure 102 is a U-shaped hole, in a possible implementation, the U-shaped hole includes a liquid inlet hole and a liquid outlet hole; the diameter of the liquid inlet hole is larger than the diameter of the liquid outlet hole.

[0049] In a possible implementation, the liquid inlet is connected to the injection pump 4 ; the liquid outlet is connected to the sample bottle 5 .

[0050] In this embodiment, the diameter of the liquid inlet is designed to be larger than that of the liquid outlet, which increases the flow rate of the fluid, ensuring smooth inflow and outflow of liquid, thereby improving liquid replacement efficiency. Furthermore, because the larger liquid inlet allows for faster introduction of new liquid, while the smaller liquid outlet allows for more effective control of liquid outflow, dead zones and stagnant areas are reduced, ensuring that old liquid is fully replaced.

[0051] When the groove structure 102 is a circular groove, in a possible implementation, the outer wall of the capillary 6 is embedded in the circular groove.

[0052] In a possible implementation, the slide system further includes a rubber sealing ring sleeved on the outer wall of the capillary 6 .

[0053] In this embodiment, the groove structure 102 is designed as a circular groove, which can be embedded with the outer wall of the capillary 6. This embedded connection can ensure the stability and sealing of the capillary 6 in the groove structure 102, thereby improving the reliability and liquid exchange efficiency of the entire system.

[0054] In addition, the rubber sealing ring provided on the outer wall of the capillary tube 6 provides an additional sealing effect to prevent leakage of the liquid during the flow process. At the same time, the rubber sealing ring can also play a certain buffering role, reducing the risk of damage to the capillary tube 6 due to vibration or impact during use.

[0055] Through the various optimization and improvement measures mentioned above, the liquid exchange efficiency of the slide system can be significantly improved, ensuring the smooth flow of liquid in the system, thereby improving the accuracy and reliability of the experiment.

[0056] During actual operation, since the plankton sample needs to flow within the sample cell space 101, this may cause the cover glass 2 and the slide 1 to shift, or the connection between the capillary 6 and the slide 1 to become loose. This situation may have an adverse effect on the experimental results, so appropriate measures must be taken to improve the stability of the system.

[0057] In a possible implementation, gel resin adhesive is filled between the capillary 6 and the two groove structures 102 .

[0058] In a possible implementation, the joint between the cover glass 2 and the slide glass 1 is filled with a gel resin adhesive.

[0059] In this embodiment, the gel resin adhesive has excellent bonding properties and can effectively secure the capillary tube 6, preventing it from shifting during sample flow. This improves the overall stability of the system and ensures the accuracy and reliability of experimental data. Furthermore, the gel resin adhesive is transparent and does not affect the observation of plankton.

[0060] In one possible implementation, Figure 3 As shown, the slide system further includes a three-way valve 8 ; the three ports of the three-way valve 8 are connected to the injection pump 4 , the sample bottle 5 and the slide 1 respectively.

[0061] In this embodiment, the three-way valve 8 can effectively control the flow path of the liquid, thereby achieving accurate injection and processing of the sample.

[0062] In one possible implementation, Figure 3 As shown, the slide system further includes a digital camera 9 for sampling and photographing the image in the objective lens 3 .

[0063] Controller 8 is connected to digital camera 9. Controller 8 sets shooting parameters, including shooting frequency, duration, and interval. Digital camera 9 automatically captures plankton morphological features at preset time points to ensure accurate capture. Furthermore, microscopic imaging system 7 collects and stores plankton morphological images for use in constructing a plankton sample library.

[0064] In this embodiment, the digital camera 9 is used to sample and photograph the image in the objective lens 3. By using the digital camera 9, researchers can observe and record the image of the sample under the microscope in real time, thereby conducting more detailed analysis and research.

[0065] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features thereof can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A slide system for constructing a plankton sample library, characterized in that: include: slides, cover slips, objectives, syringe pumps, sample vials, capillaries, microscopy imaging systems, and controllers; The slide is provided with two groove structures for accommodating capillaries for liquid to pass through; The cover glass is used to cover the slide and form a sample pool space; The sample bottle, the sample pool space and the syringe pump are connected in a capillary circulation manner; the syringe pump is used to drive the plankton sample in the sample bottle to circulate through the sample pool; The microscopic imaging system captures and displays microscopic images of plankton through the objective lens; The controller is in communication with the microscopic imaging system and is used to construct a plankton sample library based on the microscopic images.

2. The slide system according to claim 1, wherein: The groove structure is a U-shaped hole.

3. The slide system according to claim 2, wherein: The U-shaped hole includes a liquid inlet hole and a liquid outlet hole; The diameter of the liquid inlet hole is larger than the diameter of the liquid outlet hole.

4. The slide system according to claim 3, characterized in that The liquid inlet is communicated with the injection pump; the liquid outlet is communicated with the sample bottle.

5. The slide system according to claim 1, wherein: The groove structure is a circular groove, and the outer wall of the capillary is embedded in the circular groove.

6. The slide system according to claim 5, characterized in that It also includes a rubber sealing ring sleeved on the outer wall of the capillary tube.

7. The slide system according to claim 1, wherein: The space between the capillary tube and the two groove structures is filled with gel resin adhesive.

8. The slide system according to claim 1, wherein: The joint between the cover glass and the slide glass is filled with gel resin adhesive.

9. The slide system according to claim 1, wherein: It also includes a three-way valve; the three ports of the three-way valve are respectively connected to the injection pump, the sample bottle and the slide.

10. The slide system according to claim 1, wherein: A digital camera is also included for sampling and photographing the image in the objective lens.

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