Pretreatment device for biological sample

Abstract

The utility model discloses a biological sample pretreatment device which comprises a treatment cylinder, a liquid outlet is formed in the bottom end of one side of the treatment cylinder, a rotating mechanism is arranged in the middle of the bottom end in the treatment cylinder, a separation cylinder is arranged at the top end of the rotating mechanism, and limiting mechanisms are arranged in the middles of the two sides of the treatment cylinder. A sealing cover is mounted at the top end of the treatment barrel, and a feeding opening is formed in the middle of the top end of the sealing cover. The limiting groove is formed in the outer portion of the separation barrel, when the limiting clamp movably connected in the treatment barrel is clamped into the limiting groove in the separation barrel, the separation barrel can be stably limited in the treatment barrel, and by rotating a screw on the limiting mechanism, the screw can drive a connecting rod to move through a movable plate, so that the separation barrel is separated from the treatment barrel. At the moment, a connecting rod drives a limiting clamp to be pulled out of a limiting groove in the separation barrel and is opened through a sealing cover, the separation barrel can be rapidly replaced and cleaned, and meanwhile the interior of the treatment barrel can be rapidly cleaned.

Description

Technical Field

[0001] The utility model relates to the technical field of biological sample processing, in particular to a biological sample pre-processing device. Background Art

[0002] Biological samples refer to samples collected from organisms (such as animals, plants, or microorganisms) for scientific research, diagnosis, or analysis. Common biological samples include blood, urine, tissue samples, saliva, cell culture fluid, etc. These samples can be used to detect biomarkers, study disease mechanisms, evaluate drug effects, etc. A common biological sample pretreatment device is a centrifuge. It separates different components in the sample according to density through high-speed rotation and is often used in steps such as blood separation, cell precipitation, and protein extraction. This device can effectively separate the required components and provide high-quality samples for subsequent analysis and experiments.

[0003] A centrifuge device disclosed in Chinese patent publication number CN213301948U forms an independent separation liquid chamber from the inner cavity at the bottom of the outer tube by combining an outer tube boss with a filter membrane provided in a partition block. Under the action of centrifugal force, the feed and liquid can be completely separated, making the measurement results more accurate. However, the pretreatment device has low flexibility in use and is difficult to quickly clean and replace internally after use, which affects the subsequent processing effect of the biological sample and the accuracy of the centrifugal treatment. Utility Model Content

[0004] The purpose of the present invention is to provide a biological sample pretreatment device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a biological sample pretreatment device, comprising a treatment cylinder, a drain port installed at the bottom end of one side of the treatment cylinder, a rotating mechanism provided at the middle position of the bottom end inside the treatment cylinder, a separation cylinder provided at the top end of the rotating mechanism, limiting mechanisms provided at the middle positions on both sides of the treatment cylinder, a sealing cover installed at the top end of the treatment cylinder, a feed port provided at the middle position of the top end of the sealing cover, and shielding mechanisms provided on both sides of the feed port.

[0006] Preferably, the rotating mechanism comprises a driving motor installed at the middle position of the bottom end of the processing cylinder, and the output shaft end of the driving motor is installed with a cross block that is plugged into the separation cylinder.

[0007] Preferably, the limiting mechanism includes a limiting groove provided at the middle position of the exterior of the separation cylinder, screws are threadedly connected to both sides of the exterior of the treatment cylinder, and a movable plate is rotatably connected to one side of the screw.

[0008] Preferably, a connecting rod is fixedly connected to the middle position of the movable plate away from the screw rod, and a limiting clip engaged with the limiting groove is fixedly connected to one side of the connecting rod.

[0009] Preferably, an annular groove is provided inside the top end of the separation cylinder, and an insert ring which is inserted into the annular groove is fixedly connected to the bottom end of the sealing cover.

[0010] Preferably, the shielding mechanism includes blocking blocks movably connected on both sides of the feed port, one side of the blocking block is fixedly connected to a movable block that slides in the sealing cover, and one side of the movable block is fixedly connected to a spring.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] 1. This biological sample pretreatment device has a limiting groove provided on the outside of the separation cylinder. When the limiting clamp movably connected in the treatment cylinder is engaged with the limiting groove on the separation cylinder, the separation cylinder can be stably limited inside the treatment cylinder. By rotating the screw on the limiting mechanism, the screw can drive the connecting rod to move via the movable plate. At this time, the connecting rod drives the limiting clamp to be withdrawn from the limiting groove on the separation cylinder and opened through the sealing cover. The separation cylinder can be quickly replaced and cleaned, and the interior of the treatment cylinder can also be quickly cleaned. The operation is simple and convenient, and the modular design improves the flexibility and convenience of cleaning inside the treatment device.

[0013] 2. This biological sample pretreatment device has two sets of blocking blocks on both sides of the feed port. When the external transmission tube is inserted into the feed port, the blocking block is subjected to a top-down force that can squeeze the spring through the movable block, and the blocking block can be retracted into the sealing cover. When the external transmission tube is pulled out from the feed port, the movable block can push the blocking block due to the reaction force of the squeezed spring to complete the shielding of the feed port, thereby reducing the contact between the biological sample in the treatment cylinder and the external connection, and improving the safety and stability of the pretreatment device. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 This is a front view structural diagram of the utility model;

[0016] Figure 3 This is a schematic diagram of the top view of the limiting mechanism of the utility model;

[0017] Figure 4 It is a schematic diagram of the overall structure of the separation cylinder of the utility model.

[0018] In the figure: 1. Processing cylinder; 101. Discharge port; 2. Rotating mechanism; 201. Driving motor; 202. Cross block; 3. Separating cylinder; 301. Limiting groove; 302. Annular groove; 4. Limiting mechanism; 401. Screw; 402. Movable plate; 403. Connecting rod; 404. Limiting clamp; 5. Sealing cover; 501. Insert ring; 6. Feed port; 7. Shielding mechanism; 701. Blocking block; 702. Movable block; 703. Spring. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] See also Figure 1-4 , this utility model provides two technical solutions:

[0021] Embodiment 1, a biological sample pretreatment device, includes a treatment cylinder 1, a drain port 101 is installed at the bottom end of one side of the treatment cylinder 1, a rotating mechanism 2 is provided at the middle position of the bottom end of the treatment cylinder 1, a separation cylinder 3 is provided at the top of the rotating mechanism 2, and a limiting mechanism 4 is provided at the middle position of both sides of the treatment cylinder 1, a sealing cover 5 is installed at the top of the treatment cylinder 1, a feed port 6 is provided at the middle position of the top of the sealing cover 5, and shielding mechanisms 7 are provided on both sides of the feed port 6. Since the feed port 6 is provided at the middle position of the sealing cover 5, the biological sample to be separated can be poured into the separation cylinder 3 inside the treatment cylinder 1 through the feed port 6.

[0022] The rotating mechanism 2 includes a driving motor 201 installed in the middle position of the bottom end of the processing cylinder 1. The output shaft end of the driving motor 201 is installed with a cross block 202 that is inserted into the separation cylinder 3. When the driving motor 201 is started, it can drive the cross block 202 to rotate. At this time, the cross block 202 can drive the separation cylinder 3 to rotate rapidly and quickly centrifuge the internal biological samples.

[0023] The limiting mechanism 4 includes a limiting groove 301 set in the middle position of the outside of the separation cylinder 3, and screws 401 are threadedly connected on both sides of the outside of the processing cylinder 1. A movable plate 402 is rotatably connected to one side of the screw 401, and the movable plate 402 is fixedly connected to a connecting rod 403 at the middle position away from one side of the screw 401. One side of the connecting rod 403 is fixedly connected to a limiting clamp 404 that is inserted into the limiting groove 301. When the screw 401 rotates in the processing cylinder 1, the connecting rod 403 and the limiting clamp 404 can be pushed to move through the movable plate 402. When the limiting clamp 404 is stuck in the limiting groove 301 on the separation cylinder 3, the limiting work of the separation cylinder 3 can be completed.

[0024] An annular groove 302 is provided inside the top of the separation cylinder 3, and an insert ring 501 is fixedly connected to the bottom end of the sealing cover 5 and is inserted into the annular groove 302. When the sealing cover 5 is installed on the top of the treatment cylinder 1, the insert ring 501 at the bottom end of the sealing cover 5 can be inserted into the annular groove 302 at the top of the separation cylinder 3 and complete the limiting work of the top end of the separation cylinder 3.

[0025] The main difference between the second embodiment and the first embodiment is that:

[0026] A pretreatment device for biological samples, wherein the shielding mechanism 7 includes a blocking block 701 movably connected on both sides of the feed port 6, one side of the blocking block 701 is fixedly connected to a movable block 702 that slides in the sealing cover 5, and one side of the movable block 702 is fixedly connected to a spring 703. When the blocking block 701 is subjected to a force from top to bottom, the spring 703 can be squeezed by the movable block 702, and due to the reaction force of the squeezed spring 703, the blocking block 701 can be pushed to reset by the movable block 702 when the blocking block 701 is not subjected to a force. At the same time, the contents not described in detail in this specification belong to the existing technology well known to those skilled in the art.

[0027] When the embodiment of the present application is in use: the biological sample transmission tube that needs to be centrifuged is inserted into the inside of the feed port 6, and the biological sample can be poured into the separation cylinder 3 inside the treatment cylinder 1. At this time, the driving motor 201 in the treatment cylinder 1 is started, and the separation cylinder 3 can be driven to rotate by the cross block 202. At this time, the separation cylinder 3 rotates rapidly to centrifuge the internal biological sample, and allows the biological sample solution to flow into the bottom end of the inside of the treatment cylinder 1 through the through hole on the separation cylinder 3, and be concentratedly discharged through the drain port 101. At the same time, it is opened by the sealing cover 5, and the screws 401 on both sides of the treatment cylinder 1 are rotated. The screw 401 can drive the limiting clamp 404 to be pulled out of the limiting groove 301 on the separation cylinder 3 through the movable plate 402. At this time, the separation cylinder 3 can be taken out from the inside of the treatment cylinder 1, and the treatment cylinder 1 and the separation cylinder 3 can be quickly cleaned.

[0028] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely preferred examples of the present invention and are not intended to limit the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and improvements fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological sample pre-treatment device, comprising a treatment cartridge (1), characterized in that: A liquid discharge port (101) is installed at the bottom end of one side of the treatment cylinder (1), a rotating mechanism (2) is provided at the middle position of the bottom end inside the treatment cylinder (1), a separation cylinder (3) is provided at the top end of the rotating mechanism (2), a limiting mechanism (4) is provided at the middle position of both sides of the treatment cylinder (1), a sealing cover (5) is installed at the top end of the treatment cylinder (1), a feed port (6) is provided at the middle position of the top end of the sealing cover (5), and shielding mechanisms (7) are provided on both sides of the feed port (6).

2. A biological sample pretreatment device according to claim 1, characterized in that: The rotating mechanism (2) includes a driving motor (201) installed in the middle of the bottom end of the treatment cylinder (1), and a cross block (202) is installed on the output shaft end of the driving motor (201) for plugging into the separation cylinder (3).

3. The biological sample pretreatment device according to claim 1, characterized in that: The limiting mechanism (4) comprises a limiting groove (301) provided at the middle position of the outside of the separation cylinder (3); screws (401) are threadedly connected to both sides of the outside of the treatment cylinder (1); and a movable plate (402) is rotatably connected to one side of the screw (401).

4. The biological sample pretreatment device according to claim 3, characterized in that: A connecting rod (403) is fixedly connected to the middle position of the movable plate (402) away from the screw rod (401), and a limiting clamp (404) is fixedly connected to one side of the connecting rod (403) and is inserted into the limiting groove (301).

5. The biological sample pretreatment device according to claim 1, characterized in that: An annular groove (302) is provided inside the top end of the separation cylinder (3), and an inserting ring (501) that is inserted into the annular groove (302) is fixedly connected to the bottom end of the sealing cover (5).

6. The biological sample pretreatment device according to claim 1, characterized in that: The shielding mechanism (7) comprises a blocking block (701) movably connected on both sides of the feed port (6); one side of the blocking block (701) is fixedly connected to a movable block (702) that slides in the sealing cover (5); and one side of the movable block (702) is fixedly connected to a spring (703).

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