Sample purification device

Abstract

The utility model discloses a sample purification device and belongs to the technical field of biochemical equipment. The sample purification device comprises a frame body, and a pipetting module, a carrying module, an oscillation module, a suction filtration module, a centrifugation module, a monitoring module, an accommodating plate, a storage module and a recovery plate which are arranged on the frame body; the storage module is used for accommodating a reagent, the accommodating plate is used for accommodating a sample, and the suction filtration module comprises a suction filtration plate; the monitoring module is electrically connected with the pipetting module, the carrying module, the oscillation module, the suction filtration module and the centrifugation module respectively; the monitoring module enables the carrying module to be in butt joint with the containing plate, the oscillation module, the suction filtration module, the centrifugal module and the recovery plate, and the monitoring module enables the pipetting module to be in butt joint with the containing plate, the suction filtration plate and the storage module. The containing plate can be arranged on the oscillation module, the suction filtration plate can be arranged on the recovery plate, the recovery plate can recover filtered substances on the suction filtration plate, the recovery plate can be arranged on the centrifugal module and the suction filtration module, automatic operation is achieved, and the purification efficiency and quality are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical equipment, in particular to a sample purification device. Background Art

[0002] In molecular biology and biotechnology research, gel electrophoresis is a common method for recovering specific DNA or RNA fragments. However, the recovered gel fragments often contain impurities, requiring further purification to meet experimental or analytical needs. Traditional manual purification methods are cumbersome, inefficient, and prone to contamination. Therefore, a sample purification device is urgently needed to address these issues. Utility Model Content

[0003] The purpose of the utility model is to provide a sample purification device to realize automated operation, improve purification efficiency, improve purification quality, and avoid sample contamination.

[0004] To achieve this purpose, the present invention adopts the following technical solutions:

[0005] A sample purification device comprises a frame and a pipetting module, a transport module, an oscillation module, a filtration module, a centrifugal module, a monitoring module, a accommodating plate, a storage module and a recovery plate arranged on the frame; the storage module is used to accommodate reagents, the accommodating plate is used to accommodate samples, and the filtration module includes a filtration plate; the monitoring module is electrically connected to the pipetting module, the transport module, the oscillation module, the filtration module and the centrifugal module, respectively; the monitoring module enables the transport module to dock with the accommodating plate, the oscillation module, the filtration module, the centrifugal module and the recovery plate, respectively, and the monitoring module enables the pipetting module to dock with the accommodating plate, the filtration plate and the storage module, respectively; the accommodating plate can be placed on the oscillation module, the filtration plate can be placed on the recovery plate, the recovery plate can recover the filtrate on the filtration plate, and the recovery plate can be placed on the centrifugal module and the filtration module.

[0006] In some possible embodiments, the filtration module includes a first carrier and a second carrier that are detachably connected, the first carrier being capable of selectively carrying the filtration plate or the filter plate, the second carrier being empty or carrying the recovery plate or the filtration plate, an internal space and an interface being formed between one of the filtration plate and the filter plate, the first carrier and the second carrier, and the interface being connected to a vacuum pump.

[0007] In some possible embodiments, the first carrier is provided with a first limiting groove, and the second carrier is provided with a second limiting groove. When the filtration plate and the filter plate are placed on the first carrier, they can be limited to the first limiting groove. When the filtration plate or the recovery plate is placed on the second carrier, they can be limited to the second limiting groove.

[0008] In some possible embodiments, the filtration plate is provided with a plurality of first accommodating holes, and the recovery plate is provided with a plurality of second accommodating holes. When the filtration plate is provided on the recovery plate, the plurality of first accommodating holes and the plurality of second accommodating holes are provided in a one-to-one correspondence.

[0009] In some possible embodiments, the storage module includes a first container, which is used to accommodate a dissolution buffer, and the dissolution buffer can be transferred to the accommodating plate; and / or, the storage module includes a second container, which is used to accommodate an eluent, and the eluent can be transferred to the filtration plate; and / or, the storage module includes a third container, which is used to accommodate a washing liquid, and the washing liquid can be transferred to the filtration plate.

[0010] In some possible embodiments, a moving module is further included, wherein the pipetting module includes a first Z-axis moving component and a pipetting component connected to the moving end of the first Z-axis moving component, and the transport module includes a second Z-axis moving component and a transport clamp connected to the moving end of the second Z-axis moving component, and the first Z-axis moving component and the second Z-axis moving component are both arranged at the moving end of the moving module.

[0011] In some possible implementations, the moving module includes an X-axis moving component and a Y-axis moving component connected to the moving end of the X-axis moving component, and the first Z-axis moving component and the second Z-axis moving component are both arranged at the moving end of the Y-axis moving component.

[0012] In some possible embodiments, the gel block sample can be dissolved into a sample solution on the oscillation module, and the pipetting module further includes a pipette head and a power pump connected to the pipette head, wherein the pipette head is used to aspirate the reagent or the sample solution;

[0013] The pipetting head is connected to the moving end of the first Z-direction moving assembly, the power pump is provided on the frame, and the power pump and the pipetting head are connected via a conduit; or

[0014] The liquid suction head is connected to a power pump, and the power pump is connected to the moving end of the first Z-direction moving component.

[0015] In some possible implementations, the oscillation module includes a constant temperature oscillator.

[0016] In some possible implementations, the centrifugal module includes a centrifuge.

[0017] Beneficial effects of the utility model:

[0018] The utility model provides a sample purification device, which is electrically connected to the pipetting module, the transport module, the oscillation module, the filtration module and the centrifugal module through the monitoring module, and controls the above-mentioned structure to realize automated operation, that is, automatically transfer the containing plate, the recovery plate and the reagent, and automatically start the oscillation module, the filtration module and the centrifugal module, which significantly shortens the time of sample purification and improves the purification efficiency. The functional modules work closely together to ensure the continuity and stability of the purification process and improve the purification quality. In addition, the automated operation reduces human intervention and reduces the impact of human error on the experimental results. The device adopts a modular design, and each component is relatively independent, which is convenient for later maintenance and upgrading. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of a sample purification device provided in a specific embodiment of the present utility model;

[0020] Figure 2 It is a schematic diagram of a filtration module provided in a specific embodiment of the present utility model;

[0021] Figure 3 It is a top view of the filtration module provided in a specific embodiment of the present utility model;

[0022] Figure 4 yes Figure 3 AA cross-sectional view.

[0023] In the picture:

[0024] 1. Frame; 2. Pipetting module; 21. First Z-axis moving component; 22. Pipetting component; 221. Pipette head; 222. Power pump; 3. Transport module; 31. Second Z-axis moving component; 32. Transport clamp; 4. Oscillation module; 5. Filtration module; 51. First carrier; 511. First limiting groove; 52. Second carrier; 521. Second limiting groove; 53. Internal space; 54. Interface; 55. Filtration plate; 551. First accommodating hole; 552. Filter membrane; 6. Centrifugal module; 7. Recovery plate; 71. Second accommodating hole; 8. Waste liquid bottle; 9. Moving module; 91. X-axis moving component; 92. Y-axis moving component; 10. Support frame; 20. Accommodating plate; 30. Storage module. DETAILED DESCRIPTION

[0025] To make the technical problems solved by the present invention, the technical solutions adopted, and the technical effects achieved more clearly, the technical solutions of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work shall fall within the scope of protection of the present invention.

[0026] In the description of this utility model, unless otherwise specified or limited, the terms "connected," "connect," and "fixed" should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0027] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0028] like Figures 1-4As shown, the present embodiment provides a sample purification device, which comprises a rack body 1, a pipetting module 2, a carrying module 3, an oscillation module 4, a suction filtration module 5, a centrifugation module 6, a monitoring module, a containing plate 20, a storage module 30 and a recovery plate 7 arranged on the rack body 1; the storage module 30 is used for containing reagents, the containing plate 20 is used for containing samples, the suction filtration module 5 comprises a suction filtration plate 55; the monitoring module is electrically connected with the pipetting module 2, the carrying module 3, the oscillation module 4, the suction filtration module 5 and the centrifugation module 6 respectively; the monitoring module enables the carrying module 3 to be docked with the containing plate 20, the oscillation module 4, the suction filtration module 5, the centrifugation module 6 and the recovery plate 7 respectively, and enables the pipetting module 2 to be docked with the containing plate 20, the suction filtration plate 55 and the storage module 30 respectively; the containing plate 20 can be placed on the oscillation module 4, the suction filtration plate 55 can be placed on the recovery plate 7, the recovery plate 7 can recover the filtrate on the suction filtration plate 55, and the recovery plate 7 can be placed on the centrifugation module 6 and the suction filtration module 5. Optionally, the oscillation module 4 comprises a constant-temperature oscillator, the centrifugation module 6 comprises a centrifuge, and the monitoring module comprises a single-chip microcomputer or a PLC (programmable logic controller) and the like, all of which are prior art and can be purchased, and thus will not be described in detail. Optionally, the samples contained by the containing plate 20 are DNA fragments or RNA fragments.

[0029] Optionally, when the above sample purification device is used for purification, there are two ways:

[0030] The first way is negative pressure suction filtration combined with centrifugation, which comprises the following steps:

[0031] S1, the monitoring module controls the carrying module 3 to place the containing plate 20 containing the gel block-shaped sample on the oscillation module 4, and controls the oscillation module 4 to start, i.e. to accelerate the melting process of the gel block-shaped sample in the reagent by means of heating and vortex oscillation, so as to obtain a sample solution.

[0032] S2, the monitoring module controls the pipetting module 2 to transfer the melted sample solution from the containing plate 20 into the suction filtration plate 55 on the suction filtration module 5. The monitoring module controls the pipetting module 2 to be accurately docked with the containing plate 20 and the suction filtration plate 55 respectively, so as to ensure accurate transfer of the sample, avoid cross contamination, and avoid the problem of manual transfer of wrong holes.

[0033] S3, the monitoring module controls the suction filtration module 5 to start, so that the impurities and waste liquid in the sample solution are discharged into the waste liquid bottle 8 by negative pressure.

[0034] S4, the monitoring module controls the pipetting module 2 to transfer the washing liquid from the storage module 30 into the suction filtration plate 55 on the suction filtration module 5. The monitoring module controls the suction filtration module 5 to start again, so that the washing liquid in the suction filtration plate 55 is discharged into the waste liquid bottle 8 by negative pressure. This step can be repeated 1-2 times to sufficiently purify the sample.

[0035] S5. The monitoring module controls the filtration module to continue emptying so that the residual washing liquid in the filtration plate 55 is completely removed and the filter membrane in the filtration plate 55 is fully dried.

[0036] S6, the monitoring module controls the transport module 3 to transfer the filtration plate 55 from the filtration module 5 to the recovery plate 7, the monitoring module controls the pipetting module 2 to transfer the eluate from the storage module 30 to the filtration plate 55 on the filtration module 5, the monitoring module controls the transport module 3 again to transport the filtration plate 55 and the recovery plate 7 to the centrifugal module 6 at the same time, and controls the centrifugal module 6 to start, and elutes the purified sample in the filtration plate 55 through the centrifugal force generated by high-speed rotation and collects it in the recovery plate 7.

[0037] The second method: negative pressure filtration, including the following steps:

[0038] Steps S1-S5 are consistent with the first method.

[0039] S6. The monitoring module controls the transport module 3 to transfer the filtration plate 55 from the filtration module 5 to the recovery plate 7. The monitoring module controls the pipetting module 2 to transfer the eluate from the storage module 30 to the filtration plate 55 on the filtration module 5, and controls the filtration module 5 to start, and collects the purified product in the filtration plate 55 into the recovery plate 7 by negative pressure.

[0040] The monitoring module is electrically connected to the pipetting module 2, the handling module 3, the oscillation module 4, the filtration module 5 and the centrifugal module 6 respectively, and the above structure is controlled to realize automated operation, that is, automatically transferring the accommodating plate 20, the recovery plate 7 and the reagents, and automatically starting the oscillation module 4, the filtration module 5 and the centrifugal module 6, which significantly shortens the time of sample purification and improves the purification efficiency. The various functional modules work closely together to ensure the continuity and stability of the purification process and improve the purification quality. In addition, the automated operation reduces human intervention and reduces the impact of human error on the experimental results. The device adopts a modular design, and each component is relatively independent, which is convenient for later maintenance and upgrading.

[0041] like Figure 2-Figure 4 As shown, the filtration module 5 includes a first carrier 51 and a second carrier 52 that are detachably connected. The filtration plate 55 and the filter plate can be selectively carried on the first carrier 51. The second carrier 52 is used to be empty or to carry the recovery plate 7 or the filtration plate 55. An internal space 53 and an interface 54 are formed between the filtration plate 55 and the filter plate, the first carrier 51 and the second carrier 52. The interface 54 is connected to the vacuum pump. The first carrier 51 and the second carrier 52 can be freely matched to achieve multiple functions. The monitoring module controls the start-up of the vacuum pump to form a vacuum environment in the internal space 53 to achieve filtration.

[0042] In the first embodiment, the first carrier 51 carries a filtration plate 55, and the second carrier 52 carries a recovery plate 7. When the second method adopts step S6 of negative pressure filtration, the recovery plate 7 is used to collect the eluted purified product.

[0043] In the second embodiment, the first carrier 51 carries a filter plate (not shown in the figure), which is used to filter cell debris generated during plasmid extraction, and the second carrier 52 can be used to carry a filtration plate 55 for collecting the purified plasmid.

[0044] In the third embodiment, the first carrier 51 carries the filtration plate 55, and the second carrier 52 is empty. In steps S3 and S4, the monitoring module controls the start-up of the vacuum pump, so that the negative pressure environment causes molecules and impurities other than DNA in the sample solution in the filtration plate 55 to be discharged into the waste liquid bottle 8. Specifically, the impurities can be first discharged into the second carrier 52 and then discharged into the waste liquid bottle 8.

[0045] Optionally, the filtration module 5 is arranged on the frame body 1 through a support frame 10 to reduce the distance between the filtration module 5 and the pipetting module 2 and the transport module 3 .

[0046] Specifically, the filtration plate 55 is provided with a first receiving hole 551, which is filled with a filter medium, such as a filter membrane 552. The recovery plate 7 is provided with a second receiving hole 71. When the filtration plate 55 is respectively arranged on the first carrier 51 and the second carrier 52, the first receiving hole 551 and the second receiving hole 71 are connected, and multiple holes operate simultaneously, improving work efficiency. The filter medium, such as the filter membrane 552, in the first receiving hole 551 is filtered, and structures smaller than the filter hole are filtered out. Optionally, the filtration plate 55 is provided with multiple first receiving holes 551, and the recovery plate 7 is provided with multiple second receiving holes 71. When the filtration plate 55 is arranged on the recovery plate 7, the multiple first receiving holes 551 and the multiple second receiving holes 71 are arranged one-to-one, enabling simultaneous multi-hole sample operations and improving work efficiency. For example, the filtration plate 55 is provided with 96 first receiving holes 551 arranged in a 12×8 matrix, and the recovery plate 7 is provided with 96 second receiving holes 71 arranged in a 12×8 matrix. The first carrier 51 is provided with a first limiting groove 511, and the second carrier 52 is provided with a second limiting groove 521. The filtration plate 55 or the filter plate is limited to the first limiting groove 511, and the recovery plate 7 or the filtration plate 55 is limited to the second limiting groove 521, thereby ensuring that the filter plate, the filtration plate 55 and the recovery plate 7 are accurately installed in the filtration module 5, and further ensuring that when the filtration plate 55 and the recovery plate 7 are installed, the multiple first accommodating holes 551 and the multiple second accommodating holes 71 of the two correspond one to one.

[0047] The storage module 30 includes a first container for containing a dissolving buffer solution, which can be transferred to the receiving plate 20. In step S1, the pipetting module 2 adds an appropriate amount of dissolving buffer solution to the receiving plate 20 to assist in dissolving the gel block sample and improve operation efficiency.

[0048] The storage module 30 includes a second container for accommodating an eluent, which can be transferred to the filtration plate 55. In step S5, the eluent can dissolve and carry the target substance through the filtration plate 55 and be collected in the corresponding accommodating holes of the recovery plate 7.

[0049] The storage module 30 includes a third container for containing a washing liquid, which can be transferred to the filtration plate 55. After step S3, adding the washing liquid to the filtration plate 55 can further remove impurities in the sample and improve the purification effect.

[0050] The sample purification device also includes a mobile module 9. The pipetting module 2 includes a first Z-axis moving assembly 21 and a pipetting assembly 22 connected to the moving end of the first Z-axis moving assembly 21. The transport module 3 includes a second Z-axis moving assembly 31 and a transport clamp 32 connected to the moving end of the second Z-axis moving assembly 31. The first Z-axis moving assembly 21 and the second Z-axis moving assembly 31 are both located at the moving end of the mobile module 9. The transport module 3 and the pipetting module 2 are both located on the same mobile module 9, which can reduce the number of mobile modules 9, simplify the structure, and reduce costs. Furthermore, by providing the first Z-axis moving assembly 21 and the second Z-axis moving assembly 31, the transport module 3 and the pipetting module 2 can operate independently without interfering with each other.

[0051] The moving module 9 includes an X-axis moving assembly 91 and a Y-axis moving assembly 92 connected to the moving end of the X-axis moving assembly 91. The first Z-axis moving assembly 21 and the second Z-axis moving assembly 31 are both located at the moving end of the Y-axis moving assembly 92, enabling movement within a horizontal range, increasing the range of motion, facilitating docking with each module, and making the structure more compact. Optionally, the first Z-axis moving assembly 21, the second Z-axis moving assembly 31, the X-axis moving assembly 91, and the Y-axis moving assembly can all adopt structures in the prior art, such as a linear module, or a combination of a motor and a lead screw nut, etc., which will not be repeated here. In this embodiment, the X-axis, Y-axis, and Z-axis directions are perpendicular to each other.

[0052] In one embodiment, the sample in the form of gel block can be dissolved into sample solution on the oscillation module 4, the pipetting module 2 further comprises a pipetting head 221 and a power pump 222 connected with the pipetting head 221, the pipetting head 221 is used for sucking reagent or sample solution; the pipetting head 221 is connected with the moving end of the first Z-direction moving assembly 21, and the power pump 222 is connected with the moving end of the first Z-direction moving assembly 21, so that the structure is simple and compact. In another embodiment, the pipetting head 221 is connected with the moving end of the first Z-direction moving assembly 21, and the power pump 222 is arranged on the frame body 1; the power pump 222 and the pipetting head 221 are connected through a conduit, so that the load of the first Z-direction moving assembly 21 is reduced.

[0053] Obviously, the above embodiments of the present application are merely exemplary for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. For those skilled in the art, other different forms of changes or variations can be made on the basis of the above description. Here, all the embodiments are not required to be exhausted. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A sample purification device, characterized in that: The invention comprises a frame (1) and a pipetting module (2), a transport module (3), an oscillation module (4), a filtration module (5), a centrifugal module (6), a monitoring module, a accommodating plate (20), a storage module (30) and a recovery plate (7) arranged on the frame (1); the storage module (30) is used to accommodate reagents, the accommodating plate (20) is used to accommodate samples, and the filtration module (5) includes a filtration plate (55); the monitoring module is electrically connected to the pipetting module (2), the transport module (3), the oscillation module (4), the filtration module (5) and the centrifugal module (6); the monitoring module enables the transport module (3) to The monitoring module is respectively docked with the accommodating plate (20), the oscillating module (4), the suction filtration module (5), the centrifugal module (6) and the recovery plate (7); the monitoring module enables the pipetting module (2) to be respectively docked with the accommodating plate (20), the suction filtration plate (55) and the storage module (30); the accommodating plate (20) can be placed on the oscillating module (4), the suction filtration plate (55) can be placed on the recovery plate (7), the recovery plate (7) can recover the filtrate on the suction filtration plate (55), and the recovery plate (7) can be placed on the centrifugal module (6) and the suction filtration module (5).

2. The sample purification device according to claim 1, characterized in that The filtration module (5) includes a first carrier (51) and a second carrier (52) that are detachably connected. The first carrier (51) can selectively carry the filtration plate (55) or the filter plate. The second carrier (52) can be empty or carry the recovery plate (7) or the filtration plate (55). An internal space (53) and an interface (54) are formed between one of the filtration plate (55) and the filter plate, the first carrier (51) and the second carrier (52). The interface (54) is connected to a vacuum pump.

3. The sample purification device according to claim 2, characterized in that The first carrier (51) is provided with a first limiting groove (511), and the second carrier (52) is provided with a second limiting groove (521); when the filtration plate (55) and the filter plate are placed on the first carrier (51), they can be limited in the first limiting groove (511); when the filtration plate (55) or the recovery plate (7) is placed on the second carrier (52), they can be limited in the second limiting groove (521).

4. The sample purification device according to claim 1, characterized in that The suction filter plate (55) is provided with a plurality of first accommodating holes (551), and the recovery plate (7) is provided with a plurality of second accommodating holes (71). When the suction filter plate (55) is arranged on the recovery plate (7), the plurality of first accommodating holes (551) and the plurality of second accommodating holes (71) are arranged in a one-to-one correspondence.

5. The sample purification device according to claim 1, characterized in that The storage module (30) includes a first container, which is used to accommodate a dissolution buffer, and the dissolution buffer can be transferred to the accommodating plate (20); and / or, the storage module (30) includes a second container, which is used to accommodate an eluent, and the eluent can be transferred to the filtration plate (55); and / or, the storage module (30) includes a third container, which is used to accommodate a washing liquid, and the washing liquid can be transferred to the filtration plate (55).

6. The sample purification device according to claim 1, characterized in that It also includes a moving module (9), the pipetting module (2) includes a first Z-direction moving component (21) and a pipetting component (22) connected to the moving end of the first Z-direction moving component (21), the transport module (3) includes a second Z-direction moving component (31) and a transport clamp (32) connected to the moving end of the second Z-direction moving component (31), and the first Z-direction moving component (21) and the second Z-direction moving component (31) are both arranged at the moving end of the moving module (9).

7. The sample purification device according to claim 6, characterized in that: The moving module (9) includes an X-direction moving component (91) and a Y-direction moving component (92) connected to the moving end of the X-direction moving component (91), and the first Z-direction moving component (21) and the second Z-direction moving component (31) are both arranged at the moving end of the Y-direction moving component (92).

8. The sample purification device according to claim 6, characterized in that: The gel block sample can be dissolved into a sample solution on the oscillation module (4). The pipetting module (2) further comprises a pipetting head (221) and a power pump (222) connected to the pipetting head (221). The pipetting head (221) is used to absorb the reagent or the sample solution. The liquid suction head (221) is connected to the moving end of the first Z-direction moving component (21), the power pump (222) is provided on the frame (1), and the power pump (222) and the liquid suction head (221) are connected via a conduit; or The liquid suction head (221) is connected to a power pump (222), and the power pump (222) is connected to the moving end of the first Z-direction moving component (21).

9. The sample purification device according to any one of claims 1 to 8, characterized in that: The oscillation module (4) comprises a constant temperature oscillator.

10. The sample purification device according to any one of claims 1 to 8, characterized in that: The centrifugal module (6) comprises a centrifuge.

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