Tube for washing cell preparation or cells

Abstract

The utility model discloses a tube for cell preparation or cell washing, which comprises a tube body, the tube body is used for storing cell washing liquid or cell injection, a sealing plug is fixedly arranged on the inner wall of the tube body and is positioned at a position close to the top end of the tube body, the bottom end of the tube body is fixedly connected with a collecting tube and a filter membrane, and the collecting tube is fixedly connected with the filter membrane. The filter membrane is arranged in the tube body and is used for filtering impurities in the cell suspension mixed solution; the pipe clamp is arranged on the outer wall of the collecting pipe in a sleeving mode and used for closing the collecting pipe. The device has the advantages that preparation or washing operation can be completed in the closed pipe, and the high requirement for the cleanliness of the air environment is lowered to a certain degree; meanwhile, the functions of filtering and metering the liquid volume are also realized. The maximum expected value of the design is that the air cleanliness requirement of a preparation or filtering operation environment can be reduced to a certain extent on the premise of ensuring that the cell product meets the sterile requirement by using the product disclosed by the utility model.

Description

Technical Field

[0001] The utility model relates to the technical field of cell biology, in particular to a tube used for cell preparation or cell washing. Background Art

[0002] Cell injection is a widely used treatment or research method in the medical and biotechnology fields. It is achieved by injecting specific cells or processed cell suspensions into patients or experimental animals to treat diseases, promote tissue repair, or conduct scientific research. In the field of cell therapy and regenerative medicine, the preparation of cell injection is a crucial step. However, the preparation process of existing cell injection still has the following problems:

[0003] Specifically, existing cell washing tubes or preparation tubes are open tubes. To avoid contamination of cells, they can only be operated in environments with high air cleanliness, such as Class A or Class B experimental environments. That is, existing cell washing tubes or preparation tubes have high requirements for the cleanliness of the experimental operation environment, which also leads to high costs. In addition, existing cell preparation tubes or washing tubes lack precise metering functions, making it impossible to accurately control the amount of liquid during the preparation process, affecting the concentration and effect of the cell injection solution. To address the above problems, this utility model document proposes a cell preparation tube or washing tube. Utility Model Content

[0004] The purpose of the utility model is to solve the problems of traditional cell preparation tubes or washing tubes in the prior art, such as high requirements for environmental cleanliness, lack of filtering, lack of metering function, etc., and to propose a tube for cell preparation or cell washing.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a tube for cell preparation or cell washing, comprising:

[0006] A tube body, which is used to store a cell suspension for preparing a cell preparation. A sealing plug is fixedly installed on the inner wall of the tube body. The sealing plug is located near the top of the tube body and is used to complete the sealing of one end of the tube body. The bottom end of the tube body is fixedly connected to a collection tube, which is used to discharge the cell suspension mixture.

[0007] It also includes a filter membrane, which is arranged in the tube body and is used to filter impurities in the cell suspension mixture.

[0008] It also includes a pipe clamp, which is sleeved on the outer wall of the collection pipe and is used to complete the sealing of the collection pipe.

[0009] In one possible design, a support frame is fixedly installed at the bottom of the filter membrane, and the support frame is used to support the filter membrane. The support frame and the filter membrane are both located near the bottom end of the tube body, and the support frame has a mesh structure to avoid affecting the passage of liquid; the support frame and the filter membrane are tightly fitted to the inner wall of the tube body to ensure the filter membrane's interception and filtering effect on impurities.

[0010] In a possible design, an annular groove is provided on the outer wall of the support frame, a second convex ring is fixedly embedded on the inner wall of the tube body, and the annular groove and the second convex ring are engaged with each other to complete the positioning of the support frame.

[0011] In one possible design, the tube clamp includes a clamping groove opened therein, the inner wall of the clamping groove slidingly fits with the outer wall of the collection tube, and the inner diameter of the clamping groove gradually decreases from one end to the other end, so as to complete the clamping and sealing of the collection tube.

[0012] In a possible design, a first convex ring is fixedly embedded in the outer wall of the collecting tube, and the first convex ring cooperates with the bottom of the tube clamp to prevent the tube clamp from sliding and falling off.

[0013] In a possible design, an annular protrusion is fixedly mounted on the top of the pipe clamp, and the annular protrusion is used to increase the friction force in contact with the pipe clamp to ensure a pulling and sliding effect on the pipe clamp.

[0014] In a possible design, the outer wall of the tube body is provided with a scale for accurately measuring the amount of liquid in the tube body.

[0015] The pore size of the filter membrane is 10 μm or 30 μm.

[0016] When the tube body is used as a washing tube, the pore size of the filter membrane is 10 μm, and the washing liquid is used to retain cells when passing through the filter membrane; when the tube body is used as a preparation tube, the pore size of the filter membrane is 30 μm, and the cell preparation is used to retain impurities when passing through the filter membrane.

[0017] In one possible design, the sealing plug is made of rubber or silicone; the collection tube is sealed with a tearable membrane or a punctureable sealing plug. When using a tearable membrane, the membrane and its surrounding area are disinfected before use and then removed. The collection tube can then be connected to a negative pressure collection bag to collect the wash solution or cell injection solution. When using a puncture sealing plug, the surface of the puncture sealing plug is disinfected, and a syringe is passed through the puncture sealing plug, and the suction of the syringe is used to filter and extract the wash solution or cell fluid.

[0018] In one possible design, a branch tube and a tube clamp are provided on the side of the collection tube for connecting to a negative pressure collection bag. When this design is used in conjunction with a puncture sealing plug to seal the bottom of the collection tube, depending on the situation, you can choose to use a syringe to penetrate the sealing plug and use suction to filter out the washing fluid or cell fluid; you can also choose to connect to a negative pressure collection bag and use negative pressure to collect the washing fluid or cell injection fluid.

[0019] The beneficial effects of the utility model are as follows: the tube for cell preparation or cell washing provided by the utility model can complete the preparation or washing operation in the closed tube by providing a sealing plug and a tube clamp, which reduces the high requirements for environmental cleanliness to a certain extent; the tube clamp can conveniently and reliably close and open the collection tube; the sealing plug is located at the top end of the tube body and is used to complete the sealing of one end of the tube body to ensure that the liquid does not leak during the preparation process; the tube clamp sleeve is arranged on the outer wall of the collection tube, and the collection tube is clamped or loosened by sliding the clamping groove, which is simple and reliable to operate;

[0020] The utility model provides a tube for cell preparation or cell washing. By setting a scale on the outer wall of the tube, the volume of the liquid can be accurately measured, thereby accurately controlling the concentration and effect of the cell injection solution. This provides great convenience for operators during the preparation process and reduces operational difficulty and time cost.

[0021] The utility model provides a tube for cell preparation or cell washing, which can ensure the stability and filtering effect of the filter membrane by providing a support frame and an annular groove and other structures; the support frame is used to support the filter membrane to prevent the filter membrane from being deformed or damaged during the filtration process; the annular groove is engaged with the second convex ring on the inner wall of the tube body to complete the positioning of the support frame, ensure that the filter membrane is closely attached to the inner wall of the tube body, and improve the filtering effect;

[0022] In the present invention, by adopting a simple structural design and easily available materials, the production cost is reduced, so that the preparation tube can be widely promoted and applied in the fields of cell biology and biomedical research;

[0023] The utility model provides a tube for cell preparation or cell washing, which realizes the rapid preparation of cell suspension mixture in cell preparation or the automation and convenience of cell washing by ingenious combination of various components, thereby significantly improving work efficiency; the coordinated use of the filter membrane and the support frame effectively completes the filtration of impurities in the cell suspension mixture and ensures the purity of the cell injection solution; the collection tube ensures the smooth discharge of the cell mixture to avoid residue, and the scale design improves the accuracy of experimental measurement; in addition, the preparation tube has a simple structure and is easy to obtain materials, which reduces costs and is easy to be widely promoted in the fields of cell biology and biomedicine, and has extremely high practical value. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1A three-dimensional structure schematic diagram of a tube for cell preparation or cell washing is provided in the utility model;

[0025] Figure 2 A sectional structure schematic diagram of a tube for cell preparation or cell washing is provided in the utility model;

[0026] Figure 3 A tube clamp structure schematic diagram of a tube for cell preparation or cell washing is provided in the utility model;

[0027] Figure 4 An A part enlarged structure schematic diagram of a tube for cell preparation or cell washing is provided in the utility model;

[0028] Figure 5 A schematic diagram of a tube for cell preparation or cell washing is provided in another embodiment of the utility model.

[0029] In the drawing: 1, tube body; 2, collection tube; 3, sealing plug; 4, scale; 5, tube clamp; 6, clamping groove; 7, annular protrusion; 8, first protruding ring; 9, support frame; 10, filter membrane; 11, annular groove; 12, second protruding ring; 13, puncturable sealing plug; 14, valve; 15, negative pressure bag butt joint tube. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments.

[0031] Embodiment 1: refer to Figures 1-4 A cell preparation tube, comprising:

[0032] The tube body 1 is used for storage of cell suspension for preparation of cell injection solution. The sealing plug 3 is fixedly installed on the inner wall of the tube body 1 near the top end, and is used for completing the closure of one end of the tube body 1 to ensure that the liquid inside the tube body 1 will not leak. The bottom end of the tube body 1 is fixedly connected with the collection tube 2, and the collection tube 2 is used for discharging the cell suspension mixture. The collection tube 2 in the embodiment is a cell collection tube.

[0033] The tube body 1 and the collection tube 2 are made of transparent (light) material for easy observation; the sealing plug 3 has a certain thickness and can be made of rubber or silicone, which requires that the syringe is not air leakage after insertion and extraction, thereby reducing the influence of the air environment on the cell liquid in the tube.

[0034] The embodiment also includes the filter membrane 10, which is arranged in the tube body 1, and the filter hole aperture of the filter membrane 10 is 30 μm, which is used for filtering impurities in the prepared cell suspension.

[0035] This embodiment also includes a tube clamp 5, which is positioned over the outer wall of the collection tube 2 to seal the collection tube 2 and prevent leakage of the cell suspension mixture when not being discharged. The collection tube 2 is flexible enough to facilitate its closure with the tube clamp. Furthermore, the bottom of the collection tube 2 is sealed with a tearable film seal or a puncturable sealing plug.

[0036] Furthermore, to support filter membrane 10 and enhance its filtering effect on impurities, in this embodiment, a support frame 9 is fixedly mounted to the bottom of filter membrane 10. Both support frame 9 and filter membrane 10 are located near the bottom of tube body 1. Support frame 9 has a mesh structure to avoid interfering with the passage of liquid. Both support frame 9 and filter membrane 10 fit tightly against the inner wall of tube body 1, ensuring that filter membrane 10 effectively intercepts and filters impurities.

[0037] In order to complete the positioning of the support frame 9, in this embodiment, an annular groove 11 is opened on the outer wall of the support frame 9, and a second convex ring 12 is fixedly embedded on the inner wall of the tube body 1. The annular groove 11 and the second convex ring 12 are engaged with each other to ensure that the support frame 9 is stably installed in the tube body 1.

[0038] In this embodiment, the tube clamp 5 includes a clamping groove 6 defined therein. The inner wall of the clamping groove 6 slidably engages the outer wall of the collection tube 2 to securely clamp the collection tube 2. The inner diameter of the clamping groove 6 gradually decreases from one end to the other, allowing the tube clamp 5 to securely clamp the collection tube 2 and prevent it from falling off.

[0039] In this embodiment, a first convex ring 8 is fixedly embedded in the outer wall of the collecting tube 2, and the first convex ring 8 cooperates with the bottom of the tube clamp 5. When the tube clamp 5 slides to the first convex ring 8, the first convex ring 8 can prevent the tube clamp 5 from sliding further downward, thereby preventing the tube clamp 5 from sliding off.

[0040] In order to facilitate pulling and sliding the pipe clamp 5, in this embodiment, an annular protrusion 7 is fixedly installed on the top of the pipe clamp 5. The annular protrusion 7 is used to increase the friction with the fingers, so that the operator can more easily pull the pipe clamp 5 for sliding operation.

[0041] In addition, in order to accurately measure the volume of the liquid in the tube body 1, in this embodiment, a scale 4 is provided on the outer wall of the tube body 1. The operator can accurately read the amount of liquid in the tube body 1 according to the scale 4, thereby performing accurate mixed liquid preparation operations.

[0042] The working principle and usage process of this technical solution are as follows: During use, the operator can pull the tube clamp 5 horizontally to slide the collection tube 2 into the narrow area of ​​the clamping groove 6 to ensure that the collection tube 2 is clamped and closed; then the operator can use a syringe to transfer the prepared cell suspension (generally using 0.9% sodium chloride injection and 20% human serum albumin mixed with cells) into the tube body 1 in sequence. During transfer, the syringe needle is used to pierce the sealing plug 3 and complete the penetration to complete the injection of the liquid into the tube body 1. Considering the seal inside the tube body 1, there will be a certain resistance to injecting the liquid into the tube body 1. Therefore, the volume of the tube body 1 is preferably not less than 1.5 times the volume of the injection liquid; then the operator needs to filter the liquid in the tube body 1 to remove particulate impurities in the cell preparation. At this time, the collection tube collects cells. The operator disinfects the tearable film and its surrounding area, tears off the tearable film, and then connects the collection tube 2 to the cell negative pressure collection bag. The operator can then pull the tube clamp 5 in the opposite direction to complete the opening of the collection tube 2. At this time, the mixed liquid in the tube body 1 will enter the collection tube 2 through the filter membrane 10 under the action of negative pressure. The filter membrane 10 can filter out impurities contained in the cell preparation, and the remaining cell suspension will be discharged into the corresponding cell collection bag through the collection tube 2 to complete the preparation of the cell preparation.

[0043] In this embodiment, a puncture sealing plug 13 can also be used to seal the collection tube 2. When in use, after the surface of the puncture sealing plug 13 is disinfected, a syringe is passed through the puncture sealing plug 13, and the suction force of the syringe is used to filter and extract the cell fluid.

[0044] Example 2: Reference Figures 1-3 , a cell washing tube, the above-mentioned preparation tube can also be used as a cell washing tube, and the pore size of the filter membrane needs to be changed to 10μm. The collection tube 2 in this embodiment is a waste liquid collection tube; the collection tube 2 can also be sealed with a tearable film; the other structures are basically the same as Example 1.

[0045] The working principle and use process of the cell washing tube are as follows: when in use, the operator can pull the tube clamp 5 horizontally to slide the collection tube 2 into the narrow area of ​​the clamping groove 6 to ensure that the collection tube 2 is clamped and closed; then the operator can use the syringe to transfer the cell freezing solution and the configured cell washing solution into the tube body 1 in sequence. During the transfer, the syringe needle is used to pierce the sealing plug 3 and complete the penetration to complete the transfer of the liquid into the tube body 1; then the operator needs to mix the liquid in the tube body 1 evenly, and then the operator can disinfect one end of the waste liquid collection tube 2, tear off the tearable film, and connect it to the waste liquid negative pressure collection bag; then the operator can pull the tube clamp 5 in the opposite direction to complete the waste liquid collection tube 2 When the filter membrane 10 is opened, the mixed liquid in the tube body 1 will enter the waste liquid collection tube 2 through the filter membrane 10. The filter membrane 10 can intercept the cells contained in the mixed liquid, and the remaining washing waste liquid will be discharged into the waste liquid negative pressure collection bag through the waste liquid collection tube 2; then the operator can pull the tube clamp 5 again to complete the closure of the waste liquid collection tube 2, and then repeat the above operation to inject the washing liquid and discharge the waste liquid multiple times to wash the cells multiple times; finally, the operator can add a solvent to the tube body 1 to mix it with the washed cells to prepare a corresponding cell suspension. Finally, the operator can transfer the cell suspension in the tube body 1 to the cell preparation tube through a syringe for subsequent operations.

[0046] Example 3: Reference Figure 5 A tube for cell preparation or cell washing comprises a collection tube 2 having a punctureable sealing plug 13 at the bottom and a negative pressure bag connecting tube 15 at one side thereof. The negative pressure bag connecting tube 15 is provided with a valve 14. The lower edge of the negative pressure bag connecting tube 15 is required to contact the punctureable sealing plug 13 to avoid wasting cell fluid.

[0047] According to the actual situation, you can choose to use a syringe to pass through the sealing plug and use suction to filter out the washing liquid or cell fluid; you can also choose to connect it to a negative pressure collection bag and use negative pressure to collect the washing liquid or cell injection liquid.

[0048] The greatest expected value of this design lies in the use of the technical products provided by this application to reduce the air cleanliness requirements of the preparation or filtration operation environment to a certain extent while ensuring that the cell products meet the sterility requirements.

[0049] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A tube for cell preparation or cell washing, characterized in that include: A tube body (1), the tube body (1) is used for storing a cell suspension, a sealing plug (3) is fixedly installed on the inner wall of the tube body (1), the sealing plug (3) is located near the top of the tube body (1) and is used to complete the sealing of one end of the tube body (1), and the bottom end of the tube body (1) is fixedly connected to a collecting tube (2), and the collecting tube (2) is used to discharge the cell suspension mixture; It also includes a filter membrane (10), which is arranged in the tube body (1) and is used to filter impurities in the cell suspension mixture.

2. The tube for cell preparation or cell washing according to claim 1, characterized in that The tube for cell preparation or cell washing further comprises a tube clamp (5), wherein the tube clamp (5) is sleeved on the outer wall of the collection tube (2), and the tube clamp (5) is used to complete the sealing of the collection tube (2).

3. A tube for cell preparation or cell washing according to claim 1, characterized in that: A support frame (9) is fixedly installed at the bottom of the filter membrane (10), and the support frame (9) is used to support the filter membrane (10). The support frame (9) and the filter membrane (10) are both located near the bottom end of the tube body (1). The support frame (9) is a mesh structure to avoid affecting the passage of liquid; the support frame (9) and the filter membrane (10) are tightly fitted to the inner wall of the tube body (1) to ensure the filter membrane (10) has an intercepting and filtering effect on impurities.

4. A tube for cell preparation or cell washing according to claim 3, characterized in that: An annular groove (11) is provided on the outer wall of the support frame (9), and a second convex ring (12) is fixedly embedded on the inner wall of the tube body (1). The annular groove (11) and the second convex ring (12) are engaged with each other to complete the positioning of the support frame (9).

5. The tube for cell preparation or cell washing according to claim 2, characterized in that: The pipe clamp (5) includes a clamping groove (6) provided therein, wherein the inner wall of the clamping groove (6) is slidably engaged with the outer wall of the collecting pipe (2), and the inner diameter of the clamping groove (6) gradually decreases from one end to the other end, so as to complete the clamping and sealing of the collecting pipe (2).

6. The tube for cell preparation or cell washing according to claim 2, characterized in that: A first convex ring (8) is fixedly embedded in the outer wall of the collecting tube (2), and the first convex ring (8) cooperates with the bottom of the tube clamp (5) to prevent the tube clamp (5) from sliding off.

7. A tube for cell preparation or cell washing according to claim 6, characterized in that: An annular protrusion (7) is fixedly mounted on the top of the pipe clamp (5), and the annular protrusion (7) is used to increase the friction force in contact with the pipe clamp (5) to ensure a good pulling and sliding effect on the pipe clamp (5).

8. The tube for cell preparation or cell washing according to claim 1, characterized in that: The outer wall of the tube body (1) is provided with a scale (4) for accurately measuring the amount of liquid in the tube body (1); The filter pore size of the filter membrane (10) is 10 μm or 30 μm.

9. The tube for cell preparation or cell washing according to claim 7, characterized in that: The sealing plug (3) is made of rubber or silicone; the collecting tube (2) is sealed with a tearable film or a puncturable sealing plug (13).

10. The tube for cell preparation or cell washing according to claim 9, characterized in that: A negative pressure bag butt joint (15) is provided on the side of the collecting tube (2), and a valve (14) is provided on the negative pressure bag butt joint (15).

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