Liquid path system and nucleic acid sequencing system

Abstract

The utility model provides a liquid path system and a nucleic acid sequencing system.The liquid path system comprises a first container, a second container, a pump assembly and a selection valve set; the pump assembly comprises a pump body and a pump valve head, and the pump body is used for providing power to convey fluid and temporarily storing the fluid; the pump valve head is provided with a public port and a plurality of branch ports, and the public port is communicated with the pump body; the first container and the second container are both communicated with the selection valve group, and are communicated with one of the branch ports through the selection valve group; and the selection valve group selectively communicates a circulation path from the first container to the pump body or a circulation path from the pump body to the second container. According to the technical scheme, the selection valve set is arranged, so that the problems that a pump valve head is prone to abrasion, liquid leakage occurs, and normal work cannot be achieved are solved, and the service life is prolonged.

Description

Technical Field

[0001] The present application relates to the field of gene detection technology, and in particular to a liquid circuit system and a nucleic acid sequencing system. Background Art

[0002] The liquid system of the gene sequencer is powered by a pump, which draws the target reagent in the reagent pool into the fluid channel of the liquid system and passes the target reagent into the predetermined flow channel; the liquid system can also perform gas suction, discharge and circulation to achieve specific functions or uses.

[0003] The pump includes a pump body and a pump valve head. The pump valve head has multiple ports, one of which is a common port, which is connected to the pump body. The other multiple ports can be connected to multiple flow channels or other channels at the same time. The common port of the pump is connected to the remaining ports through the flow channel inside the pump valve head. This internal flow channel can be rotated to achieve communication between the common port and the remaining ports.

[0004] During operation, the flow channel in the rotating pump valve head is connected to different ports to realize actions such as suction and discharge of liquid. Frequent rotation for a long time can easily cause wear of the stator and rotor. In addition, there will be residual reagents in the pump valve head, and solid crystals will form after the reagents dry. Frequent rotation for a long time can easily cause wear of the stator and rotor, resulting in a shortened service life of the pump valve head and prone to leakage and other related problems. Utility Model Content

[0005] The present application provides a fluid circuit system and a nucleic acid sequencing system to reduce the wear problem of the pump valve head and extend the service life.

[0006] The present application provides a fluid circuit system, comprising a first container, a second container, a pump assembly, and a selection valve group;

[0007] The pump assembly includes a pump body and a pump valve head, wherein the pump body is used to provide power to transport the fluid and temporarily store the fluid;

[0008] The pump valve head is provided with a common port and a plurality of sub-ports, wherein the common port is in communication with the pump body;

[0009] Both the first container and the second container are in communication with the selection valve group, and are in communication with one of the branch ports through the selection valve group;

[0010] The selection valve group selectively connects the flow path from the first container to the pump body or the flow path from the pump body to the second container.

[0011] In a specific embodiment, the selection valve group forms a one-way flow path from the first container to the pump body, and a one-way flow path from the pump body to the second container.

[0012] In a specific embodiment, the selection valve group includes a three-way valve, a first regulating valve and a second regulating valve;

[0013] The three ports of the three-way valve are respectively connected to the first container, the second container and the branch port;

[0014] The first regulating valve and the second regulating valve are both one-way valves;

[0015] The first regulating valve is connected between the three-way valve and the first container, and allows the fluid to flow from the first container to the three-way valve in one direction;

[0016] The second regulating valve is connected between the three-way valve and the second container, and allows the fluid to flow from the three-way valve to the second container in a one-way manner.

[0017] In a specific embodiment, the selection valve group is connected to the branch port via one of the first container and the second container.

[0018] In a specific embodiment, the selection valve group is a multi-way solenoid valve, and the multi-way solenoid valve is provided with at least three connection ports, namely a first connection port, a second connection port and a third connection port;

[0019] The first connection port is in communication with the first container, the second connection port is in communication with the second container, and the third connection port is in communication with the branch port;

[0020] The multi-way solenoid valve connects the first connection port and the second connection port to the third connection port.

[0021] In a specific embodiment, the selection valve group includes a three-way valve, a first regulating valve and a second regulating valve;

[0022] The three ports of the three-way valve are respectively connected to the first container, the second container and the branch port;

[0023] The first regulating valve is connected between the three-way valve and the first container, and the second regulating valve is connected between the three-way valve and the second container;

[0024] The first regulating valve and the second regulating valve are both solenoid valves.

[0025] In a specific embodiment, the first container is a flow channel, and the second container is a waste liquid tank.

[0026] In a specific possible implementation scheme, there are multiple flow channels, and each flow channel is correspondingly provided with a selection valve group;

[0027] Each selection valve group is respectively connected to a different branch port on the pump valve head.

[0028] In a specific embodiment, it further comprises a reagent pool;

[0029] The plurality of flow channels are divided into a plurality of groups, and each group includes a plurality of the flow channels;

[0030] The pump components are also provided in plurality and are provided corresponding to the plurality of groups of the flow channels, and the plurality of the flow channels in each group are respectively connected to different branch ports on the same pump valve head through the selection valve group;

[0031] One of the flow channels in each group is connected in parallel with one of the flow channels in another group and then communicated with the reagent pool.

[0032] In a second aspect, the present application provides a nucleic acid sequencing system, comprising an optical detection system, a computer system, and a liquid system as described in any one of the above items;

[0033] The liquid system receives a sequencing object, and the sequencing object contacts a reagent and undergoes a chemical reaction so as to carry a fluorescent label;

[0034] The optical detection system is configured to excite the fluorescent marker carried by the sequencing object and detect the fluorescent signal generated by the excited fluorescent marker;

[0035] The computer system is configured to obtain a fluorescent image from the optical detection system and identify the gene sequence of the sequencing object based on the fluorescent image. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 A structural block diagram of the nucleic acid sequencing system provided in an embodiment of the present application;

[0037] Figure 2 A schematic diagram of the structure of the fluid circuit system provided in an embodiment of the present application;

[0038] Figure 3 Schematic diagram of the connection between the selection valve group and the pump valve head provided in an embodiment of the present application. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solutions and advantages of this application clearer, this application will be further described in detail below with reference to the accompanying drawings.

[0040] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in one or more embodiments of this specification should have the usual meanings understood by people with ordinary skills in the field to which this disclosure belongs. The "first", "second" and similar words used in one or more embodiments of this specification do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative position relationships. When the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0041] To facilitate understanding of the liquid circuit system and nucleic acid sequencing system provided in the embodiment of the present application, its application scenario is first explained. The liquid circuit system provided in the embodiment of the present application is applied to a gene detector for sequencing by reacting the object to be sequenced with the reagent. The liquid circuit system includes a flow channel and a pump assembly. The flow channel is the main location for the reaction, and the pump assembly is used to provide power for transporting fluids such as reagents; wherein the pump assembly includes a pump body and a pump valve head, and the pump valve head is provided with multiple ports, one of which is a common port, which is connected to the pump body, and the other multiple ports are respectively connected to the flow channel. In addition, some ports can be set to be connected to a waste liquid pool, a buffer solution, etc.

[0042] During actual operation, the pump valve head rotates the internal flow channel to connect the common port with multiple other ports, so as to perform actions such as suction and discharge of liquid according to actual needs. During the adjustment of the pump valve head, the stator and rotor inside it rotate relative to each other, generating friction. As the use time increases, the degree of wear becomes increasingly severe, which can easily cause leakage in the pump valve head and greatly shorten its service life.

[0043] Moreover, after the pump stops working, part of the fluid it transports (mainly reaction reagents) will remain in it. The remaining part will crystallize, which will further aggravate the wear between the stator and rotor. As the amount of crystallization increases, it may even cause jamming and prevent normal use.

[0044] To this end, the present invention provides a fluid circuit system and a nucleic acid sequencing system to alleviate the wear and tear of the pump valve head and the problem of improper operation, thereby extending the service life. A detailed description of the system is provided below with reference to specific figures and embodiments.

[0045] See also Figure 1 , Figure 1 This is an overall architecture diagram of a nucleic acid sequencing system provided by an embodiment of the present disclosure. Figure 1 As shown, the nucleic acid sequencing system 100 provided by the embodiment of the present disclosure comprises a liquid channel system 10, a chip platform 20, an optical detection system 30 and a computer system 40.

[0046] The liquid channel system 10 is attached with one or more sequencing objects; the flow channel in the liquid channel system receives the sequencing objects, the sequencing objects are in contact with reagents and chemical reactions occur so as to carry fluorescent labels;

[0047] The chip platform 20 is configured to fix and support the flow channel pool of the liquid channel system 10.

[0048] The optical detection system 30 is configured to excite the fluorescent labels in the reagents for sequencing, and detect the fluorescent signals generated by the excited fluorescent labels.

[0049] The computer system 40 is configured to acquire fluorescent images from the optical detection system 30, and identify the nucleic acid sequence of the sequencing object according to the acquired fluorescent images.

[0050] Reference Figure 2 And Figure 3 The liquid channel system provided by the embodiment of the present disclosure comprises a first container, a second container, a pump assembly 1, a selection valve group 2 and a reagent pool 3. The pump assembly 1 comprises a pump body 11 and a pump valve head 12. The pump body 11 is taken as an example of a syringe pump and can temporarily store liquid. The pump valve head 12 is provided with a common port 121 and a plurality of branch ports 122. The common port 121 is in communication with the pump body 11. The first container and the second container are both in communication with the selection valve group 2 and are in communication with one of the branch ports 122 through the selection valve group 2, forming a fluid passage from the first container to the second container. The selection valve group 2 selectively communicates a flow passage from the first container to the pump body 11 or a flow passage from the pump body 11 to the second container.

[0051] The first container is a flow channel 4, which is in communication with the reagent pool 3 through a pipeline to transport target reagents in the reagent pool 3 into the flow channel 4 for reaction. The second container is a waste liquid pool 5 for storing reagents after reaction or excess reagents. In other embodiments, the first container can also be a cleaning liquid container or a buffer liquid container, and the second container is the flow channel 4. In the embodiment of the present disclosure, the first container is taken as an example of the flow channel 4 and the second container is taken as an example of the waste liquid pool 5.

[0052] In one embodiment, the selection valve group 2 forms a one-way flow passage from the flow channel 4 to the pump body 11 and a one-way flow passage from the pump body 11 to the waste liquid pool 5. That is, the selection valve group 2 communicates the flow passage from the flow channel 4 to the pump body 11 and the flow passage from the pump body 11 to the waste liquid pool 5.

[0053] Specifically, referring to Figure 3 and combining Figure 2 The selection valve group 2 includes a three-way valve 21, a first regulating valve 22 and a second regulating valve 23. The first regulating valve 22 and the second regulating valve 23 are both one-way valves; the three ports of the three-way valve 21 are respectively connected to the flow channel 4, the waste liquid tank 5 and the branch port 122; the first regulating valve 22 is connected between the three-way valve 21 and the flow channel 4, and allows the fluid to flow from the flow channel 4 to the three-way valve 21 in one direction; the second regulating valve 23 is connected between the three-way valve 21 and the waste liquid tank 5, and allows the fluid to flow from the three-way valve 21 to the waste liquid tank 5 in one direction.

[0054] In this way, during actual use, there is no need to manually adjust the selection valve group 2, and the path from the flow channel 4 to the branch port 122 and from the branch port 122 to the waste liquid pool 5 is always in a connected state; the pump body 11 works to provide power to transport the target reagent from the reagent pool 3 to the flow channel 4. After the reaction is completed, the pump body 11 sucks the reagent in the flow channel 4, and this part of the reagent flows into the pump body 11 through the first regulating valve 22, the three-way valve 21, and the pump valve head 12; then the working state of the pump body 11 is changed, and the reagent in the pump body 11 is reversely transported so that it flows into the waste liquid pool 5 through the pump valve head 12, the three-way valve 21, and the second regulating valve 23. Since the first regulating valve 22 and the second regulating valve 23 are both one-way valves, the reagent can only flow along the above-mentioned path, and there is no need to manually adjust the selection valve group 2 or change its working state during the transportation process, which improves the convenience of use and achieves more stable functions.

[0055] In another embodiment, the selection valve group 2 connects the flow channel 4 and the waste liquid pool 5 to the branch port 122, that is, the selection valve group connects the flow path from the first container to the pump body 11, or the flow path from the pump body 11 to the second container.

[0056] Specifically, the selection valve group 2 is set as a multi-way solenoid valve, which is provided with at least three connection ports, namely a first connection port, a second connection port and a third connection port; the first connection port is connected to the flow channel 4, the second connection port is connected to the waste liquid tank 5, and the third connection port is connected to the branch port 122; the multi-way solenoid valve selectively connects the first connection port and the second connection port to the third connection port, thereby realizing the regulation of selectively connecting the flow channel 4 and the waste liquid tank 5 to the pump assembly 1.

[0057] In this way, the reagent is transported in coordination with the change in the working state of the pump body 11; for example, when the target reagent is passed from the reagent pool 3 into the flow channel 4, the first connection port of the multi-way solenoid valve is connected to the third connection port, and the second connection port is disconnected from the third connection port; when the reagent is transported from the pump body 11 to the waste liquid pool 5, the first connection port is disconnected from the third connection port, and the second connection port is connected to the third connection port.

[0058] In addition, the selection valve group 2 can be provided with the three-way valve 21, the first regulating valve 22 and the second regulating valve 23, wherein the first regulating valve 22 and the second regulating valve 23 are both electromagnetic valves; the specific connection structure can refer to the connection structure of the three-way valve 21, the first regulating valve 22 and the second regulating valve 23 in the previous embodiment and the flow channel 4, the waste liquid pool 5 and the pump assembly 1, and the difference is that the first regulating valve 22 and the second regulating valve 23 are both electromagnetic valves.

[0059] The first regulating valve 22 and the second regulating valve 23 are both electromagnetic valves, which are connected with the control system to control the opening and closing of the valves through the electric control system, so as to selectively form a flow path from the flow channel 4 to the pump body 11 or a flow path from the pump body 11 to the waste liquid pool 5.

[0060] For example, when the target reagent is introduced into the flow channel 4 from the reagent pool 3, the first regulating valve 22 is opened and the second regulating valve 23 is closed; when the reagent is delivered from the pump body 11 to the waste liquid pool 5, the first regulating valve 22 is closed and the second regulating valve is opened.

[0061] Through the above arrangement, when the reagent needs to be delivered between the flow channel 4, the pump body 11 and the waste liquid pool 5, the common port 121 of the pump valve head 12 does not need to be connected with different branch ports 122 to form different channels through the adjustment of the pump valve head 12, but the flow path is adjusted through the additionally provided selection valve group 2, so that the problem of serious wear of the rotor and the stator caused by frequent adjustment of the pump valve head 12 can be alleviated, the problems of liquid leakage and failure to work normally can be reduced, and the service life of the pump valve head 12 and the pump assembly 1 can be prolonged.

[0062] In addition to the above, a third container can also be provided, for example, the third container is a cleaning liquid container or a buffer liquid container, and different containers are connected with the same branch port 122 through the adjustment of the selection valve group 2, so as to realize the transmission of liquid between multiple different containers.

[0063] Reference Figure 2 and Figure 3 The flow channel 4 has multiple flow channels, and each flow channel 4 is provided with a selection valve group 2, and each selection valve group 2 is connected with a different branch port 122 of the pump valve head 12; the number of the waste liquid pools 5 can be set to one for each flow channel 4, or one for multiple flow channels 4, which can be set according to actual needs.

[0064] The multiple flow channels 4 are divided into multiple groups, and each group also includes multiple flow channels 4. Correspondingly, the pump assembly 1 is also provided with multiple, one-to-one correspondences with the multiple groups of flow channels 4. The multiple flow channels 4 in each group are respectively connected to different branch ports 122 on the same pump valve head 12 through corresponding selection valve groups 2. One of the flow channels 4 in each group of flow channels 4 is connected in parallel with one of the flow channels 4 in other groups and then connected to the reagent cell 3. That is, the multiple flow channels 4 connected in parallel to a reagent cell 3 are distributed in each group.

[0065] For example, refer to Figure 2 There are four flow channels 4 in total, two of which form a group and are connected to the same pump assembly 1, through which power is provided; one of the flow channels 4 of the first group is connected in parallel with one of the flow channels 4 of the second group, and then connected to the reagent pool 3, and the remaining two flow channels 4 are connected in parallel to the reagent pool 3.

[0066] In addition, the fluid circuit system is further provided with a control valve 6, which is provided with multiple ports, including at least one liquid inlet port and at least one liquid outlet port. The aforementioned multiple parallel flow channels 4 are connected in parallel to a liquid outlet port of the control valve 6. When multiple liquid inlet ports are provided, the liquid outlet port of the control valve 6 selectively connects to different liquid inlet ports. Thus, when multiple reagent cells 3 are provided, the control valve 6 is provided with multiple liquid inlet ports, each connected to a different reagent cell 3, to deliver different target reagents to the flow channel 4.

[0067] In addition, when multiple liquid outlet ports are provided, other liquid outlet ports can be connected to other flow channels 4 or waste liquid tanks 5, etc.

[0068] The one or more embodiments of this specification are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of one or more embodiments of this specification shall be included within the scope of protection of this disclosure.

[0069] The above are only specific embodiments of the present application, but the scope of protection of this application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A fluid system, characterized in that: It includes a first container, a second container, a pump assembly and a selection valve group; The pump assembly includes a pump body and a pump valve head, wherein the pump body is used to provide power to transport the fluid and temporarily store the fluid; The pump valve head is provided with a common port and a plurality of sub-ports, wherein the common port is in communication with the pump body; The first container and the second container are both connected to the selection valve group and are connected to one of the sub-ports through the selection valve group; The selection valve group selectively connects the flow path from the first container to the pump body or the flow path from the pump body to the second container.

2. The fluid circuit system according to claim 1, characterized in that: The selector valve group forms a one-way flow path from the first container to the pump body, and a one-way flow path from the pump body to the second container.

3. The fluid circuit system according to claim 2, characterized in that: The selection valve group includes a three-way valve, a first regulating valve and a second regulating valve; The three ports of the three-way valve are respectively connected to the first container, the second container and the branch port; The first regulating valve and the second regulating valve are both one-way valves; The first regulating valve is connected between the three-way valve and the first container, and allows the fluid to flow from the first container to the three-way valve in one direction; The second regulating valve is connected between the three-way valve and the second container, and allows the fluid to flow from the three-way valve to the second container in a one-way manner.

4. The fluid circuit system according to claim 1, wherein: The selection valve group is connected to the branch port by selecting one of the first container and the second container.

5. The fluid circuit system according to claim 4, characterized in that: The selection valve group is a multi-way solenoid valve, and the multi-way solenoid valve is provided with at least three connection ports, namely a first connection port, a second connection port and a third connection port; The first connection port is in communication with the first container, the second connection port is in communication with the second container, and the third connection port is in communication with the branch port; The multi-way solenoid valve connects the first connection port and the second connection port to the third connection port.

6. The fluid circuit system according to claim 4, characterized in that: The selection valve group includes a three-way valve, a first regulating valve and a second regulating valve; The three ports of the three-way valve are respectively connected to the first container, the second container and the branch port; The first regulating valve is connected between the three-way valve and the first container, and the second regulating valve is connected between the three-way valve and the second container; The first regulating valve and the second regulating valve are both solenoid valves.

7. The fluid path system according to any one of claims 1 to 6, characterized in that: The first container is a flow channel, and the second container is a waste liquid pool.

8. The fluid circuit system according to claim 7, characterized in that: There are multiple flow channels, and each flow channel is correspondingly provided with a selection valve group; Each selection valve group is respectively connected to a different branch port on the pump valve head.

9. The fluid circuit system according to claim 8, characterized in that: Also includes a reagent cell; The plurality of flow channels are divided into a plurality of groups, and each group includes a plurality of the flow channels; The pump components are also provided in plurality and are provided corresponding to the plurality of groups of the flow channels, and the plurality of flow channels in each group are respectively connected to different branch ports on the same pump valve head through the selection valve group; One of the flow channels in each group of flow channels is connected in parallel with one of the flow channels in other groups of flow channels and then communicated with the reagent pool.

10. A nucleic acid sequencing system, characterized in that: comprising an optical detection system, a computer system, and a fluid system according to any one of claims 1 to 9; The liquid system receives a sequencing object, and the sequencing object contacts a reagent and undergoes a chemical reaction so as to carry a fluorescent label; The optical detection system is configured to excite the fluorescent marker carried by the sequencing object and detect the fluorescent signal generated by the excited fluorescent marker; The computer system is configured to obtain a fluorescent image from the optical detection system and identify the gene sequence of the sequencing object based on the fluorescent image.

Images