Tumor cell scratching auxiliary device

Abstract

The utility model provides a tumor cell scratching auxiliary device, which comprises a rectangular frame, an elastic limiting piece and a scratching plate, the rectangular frame is used for being sleeved on the periphery of a cell culture plate, two opposite side walls of the rectangular frame are respectively provided with a clamping groove in a penetrating manner, and the clamping grooves extend upwards to the upper end face of the rectangular frame; the elastic limiting piece is arranged on the side part of the rectangular frame, penetrates through the rectangular frame and extends into the rectangular frame, and the elastic limiting piece can elastically abut against the side wall of the cell culture plate to lock the position of the rectangular frame; the scratching plate is horizontally arranged in the rectangular frame, the edges of the two opposite sides of the scratching plate are provided with wing plates located in the two clamping grooves in a one-to-one correspondence mode, and scratching holes which extend horizontally and are used for being opposite to culture holes of the cell culture plate are formed in the scratching plate in a penetrating mode. According to the tumor cell scratching auxiliary device provided by the utility model, the straightness of scratches is ensured, and the influence on the observation of experimental results is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell experiment equipment, and more specifically relates to a tumor cell scratching auxiliary device. Background Art

[0002] The cell scratch assay is widely used in the biomedical field and is an important experimental method for studying the effects of drugs and other substances on cell migration. The existing cell scratch assay method is to culture an adherent monolayer of cells in a cell culture plate, then use a micropipette tip to create a scratch wound perpendicular to the plate. The cells are then rinsed with PBS, cultured with culture medium for a specified period of time, and photographed.

[0003] However, when manually scratching with a micropipette tip, the scratching is usually done only by feel, and the straightness of the scratch cannot be guaranteed, and it may even be skewed and distorted, affecting the observation of experimental results. Utility Model Content

[0004] The embodiment of the utility model provides a tumor cell scratching auxiliary device, which ensures the straightness of the scratch and avoids affecting the observation of experimental results.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: to provide a tumor cell scratching auxiliary device, including a rectangular frame, an elastic limiter and a scratching plate, the rectangular frame is used to be sleeved on the outer periphery of the cell culture plate, and the opposite side walls of the rectangular frame are penetrated by a card slot, and the card slot extends upward to the upper end face of the rectangular frame; the elastic limiter is arranged on the side of the rectangular frame and is penetrated by a limiter extending into the rectangular frame, and the elastic limiter can elastically abut against the side wall of the cell culture plate to lock the position of the rectangular frame; the scratching plate is horizontally arranged in the rectangular frame, and the opposite side edges of the scratching plate are provided with wing plates corresponding to each other in the two card slots, and the scratching plate is penetrated by a horizontally extending scratch hole for being arranged opposite to the culture hole of the cell culture plate.

[0006] In one possible implementation, a mounting seat is provided on the outside of the rectangular frame, and the mounting seat has a mounting cavity that passes through from top to bottom. The elastic limit member includes a limit column and an elastic member. The limit column passes through the mounting seat and the side of the rectangular frame and extends into the rectangular frame, and slides with the mounting seat and the rectangular frame. A limit boss protruding toward the periphery is provided in the middle of the limit column, and the limit boss is located in the mounting cavity; the elastic member is sleeved on the periphery of the limit column and is located between the limit boss and the inner wall of the mounting cavity, and is used to elastically push the limit column to abut against the side wall of the cell culture plate to lock the position of the rectangular frame.

[0007] In some embodiments, the outer end of the limiting column is provided with a force application platform protruding toward the outer periphery.

[0008] In some embodiments, the inner end of the limiting post is connected to a strip plate for abutting against the side wall of the cell culture plate.

[0009] In a possible implementation, an iron sheet is embedded on the inner bottom wall of the slot, and a magnet for attracting the iron sheet is embedded on the bottom surface of the wing plate.

[0010] In a possible implementation, there are four card slots, which are arranged in a one-to-one correspondence with the four side walls of the rectangular frame; there are four wing plates, which are arranged in a one-to-one correspondence with the four card slots.

[0011] In a possible implementation, the scratch holes are arranged in a plurality of groups at intervals along a direction perpendicular to the extending direction thereof, and each group of scratch holes is provided with a plurality of scratch holes at intervals along the direction perpendicular to the extending direction thereof.

[0012] Compared with the prior art, the tumor cell scratching auxiliary device provided in this embodiment is different from the prior art. When cells need to be scratched, a rectangular frame is put on the outer periphery of the cell culture plate, and the elastic limiter elastically abuts against the side wall of the cell culture plate to lock the position of the rectangular frame. Then the scratching plate is placed in the rectangular frame, and the wing plate is embedded in the card slot. The cells are scratched along the length direction of the scratch hole with a micropipette tip through the scratch hole, ensuring the straightness of the scratch and avoiding affecting the observation of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments or descriptions of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0014] Figure 1 A schematic diagram of the structure of the tumor cell scratching auxiliary device provided by an embodiment of the present utility model in use;

[0015] Figure 2 A schematic structural diagram of the tumor cell scratching assisting device provided by an embodiment of the present invention from another perspective;

[0016] Figure 3 A schematic diagram of the explosion structure of the tumor cell scratching auxiliary device provided by an embodiment of the utility model;

[0017] Figure 4 A schematic diagram of the exploded structure of the tumor cell scratching auxiliary device provided in an embodiment of the present utility model from another perspective.

[0018] Among them, the reference numerals in the figures are:

[0019] 1, cell culture plate; 2, culture hole; 10, rectangular frame; 11, clamping groove; 12, iron sheet; 20, elastic limiting piece; 21, limiting column; 22, limiting boss; 23, elastic piece; 24, force applying table; 25, strip plate; 30, scratch plate; 31, wing plate; 32, scratch hole; 33, magnet; 40, mounting seat; 41, mounting cavity. DETAILED DESCRIPTION

[0020] In order to make the technical problems, technical schemes and beneficial effects of the utility model clearer, the utility model will be further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and not to limit the utility model.

[0021] It should be noted that when an element is referred to as being "disposed on" another element, it can be directly on the other element or indirectly on the other element. It should be understood that the terms "length", "width", "upper", "lower", "front", "back", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model. The terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features. In the description of the utility model, the meaning of "several" is two or more than two, unless otherwise explicitly specified.

[0022] Please refer to Figures 1 to 4 The tumor cell scratch auxiliary device provided by the utility model will be described. The tumor cell scratch auxiliary device comprises a rectangular frame 10, an elastic limiting piece 20 and a scratch plate 30. The rectangular frame 10 is used for sleeving the outer periphery of the cell culture plate 1. Clamping grooves 11 are formed through the opposite two side walls of the rectangular frame 10 and extend upward to the upper end face of the rectangular frame 10. The elastic limiting piece 20 is arranged on the side of the rectangular frame 10 and extends into the rectangular frame 10. The elastic limiting piece 20 can elastically abut against the side wall of the cell culture plate 1 to lock the position of the rectangular frame 10. The scratch plate 30 is horizontally arranged in the rectangular frame 10. The opposite two side edges of the scratch plate 30 are provided with wing plates 31 corresponding to the two clamping grooves 11. The scratch plate 30 is provided with scratch holes 32 extending horizontally and arranged opposite to the culture holes 2 of the cell culture plate 1.

[0023] Further, the two clamping grooves 11 are arranged opposite to each other.

[0024] An embodiment of the present application provides a tumor cell scratching auxiliary device. During its actual use, when cells need to be scratched, the rectangular frame 10 is put on the outer periphery of the cell culture plate 1, so that the elastic limiter 20 elastically abuts against the side wall of the cell culture plate 1 to lock the position of the rectangular frame 10, and then the scratching plate 30 is placed in the rectangular frame 10, and the wing plate 31 is embedded in the card slot 11. The cells are scratched along the length direction of the scratching hole 32 with a micropipette tip through the scratching hole 32, ensuring the straightness of the scratch and avoiding affecting the observation of the experimental results.

[0025] After the scratching is completed, the elastic limiter 20 is released from the side wall of the cell culture plate 1, and the rectangular frame 10 is separated from the cell culture plate 1. Then, the outer edges of the two wing plates 31 can be pinched one by one with the thumb and index finger of one hand, and the scratching plate 30 can be lifted up to remove the scratching plate 30 from the rectangular frame 10, which facilitates the separation of the scratching plate 30 from the rectangular frame 10.

[0026] Compared with the prior art, the tumor cell scratching auxiliary device provided in this embodiment is different from the prior art. When cells need to be scratched, the rectangular frame 10 is put on the outer periphery of the cell culture plate 1, so that the elastic limiter 20 elastically abuts against the side wall of the cell culture plate 1 to lock the position of the rectangular frame 10, and then the scratching plate 30 is placed in the rectangular frame 10, and the wing plate 31 is embedded in the card slot 11. The cells are scratched along the length direction of the scratch hole 32 with a micropipette tip through the scratch hole 32, ensuring the straightness of the scratch and avoiding affecting the observation of experimental results.

[0027] In a possible implementation, the rectangular frame 10 is formed as follows: Figures 1 to 4 The structure shown, see Figures 1 to 4 A mounting seat 40 is provided on the outside of the rectangular frame 10, and the mounting seat 40 has a mounting cavity 41 that passes through from top to bottom. The elastic limit member 20 includes a limit column 21 and an elastic member 23. The limit column 21 passes through the side of the mounting seat 40 and the rectangular frame 10 and extends into the rectangular frame 10, and slides with the mounting seat 40 and the rectangular frame 10. A limit boss 22 protruding toward the periphery is provided in the middle of the limit column 21, and the limit boss 22 is located in the mounting cavity 41; the elastic member 23 is sleeved on the outer periphery of the limit column 21 and is located between the limit boss 22 and the inner wall of the mounting cavity 41, and is used to elastically push the limit column 21 to abut against the side wall of the cell culture plate 1 to lock the position of the rectangular frame 10.

[0028] Specifically, the limiting post 21 not only penetrates the side wall of the mounting base 40 away from the rectangular frame 10, but also penetrates the side wall of the rectangular frame 10 near the mounting base 40, and extends into the rectangular frame 10. The limiting post 21 is not only slidably connected to the mounting base 40, but also slidably connected to the rectangular frame 10. The end of the limiting boss 22 near the rectangular frame 10 is used to abut against the side wall of the rectangular frame 10 for limiting position.

[0029] When the rectangular frame 10 needs to be installed, the limiting column 21 is pulled outward so that the inner end of the limiting column 21 moves in the direction of being retracted into the mounting seat 40, the elastic member 23 is compressed, and then the rectangular frame 10 is sleeved on the outer periphery of the cell culture plate 1. After the position of the rectangular frame 10 is adjusted, the limiting column 21 is released. The inner end of the limiting column 21 abuts against the outer wall of the cell culture plate 1 under the elastic push of the elastic member 23, thereby locking the relative position of the rectangular frame 10 and the cell culture plate 1.

[0030] In some embodiments, see Figures 1 to 4 The outer end of the limiting column 21 is provided with a force application platform 24 protruding toward the outer periphery.

[0031] Specifically, when the staff needs to pull the limiting column 21, they can apply force to the force application platform 24 to move the limiting column 21, thereby improving the convenience of use.

[0032] In some embodiments, see Figures 2 to 4 The inner end of the limiting column 21 is connected to a strip plate 25 for abutting against the side wall of the cell culture plate 1 .

[0033] Specifically, the strip plate 25 extends along the extension direction of the side wall of the rectangular frame 10 (the side wall of the rectangular frame 10 opposite to the strip plate 25). The strip plate 25 can increase the contact area with the side wall of the rectangular frame 10 on the basis of the limiting column 21, thereby improving the stability of position locking.

[0034] In a possible implementation, the card slot 11 is configured as follows: Figure 3 and Figure 4 The structure shown, see Figure 3 and Figure 4 An iron sheet 12 is embedded on the inner bottom wall of the slot 11 , and a magnet 33 for adsorbing the iron sheet 12 is embedded on the bottom surface of the wing plate 31 .

[0035] Specifically, after the wing plate 31 is placed in the slot 11, the magnet 33 is adsorbed by the iron sheet 12, so that the scratching plate 30 is stably placed on the rectangular frame 10, avoiding the micro-pipette tip accidentally driving the scratching plate 30 to move upward during the scratching process, thereby improving the stability of the position of the scratching plate 30 during the scratching process.

[0036] In a possible implementation, the card slot 11 is configured as follows: Figure 1 、 Figure 3 and Figure 4 The structure shown, see Figure 1 、 Figure 3 and Figure 4 There are four card slots 11 , which are arranged in a one-to-one correspondence with the four side walls of the rectangular frame 10 , and there are four wing plates 31 , which are arranged in a one-to-one correspondence with the four card slots 11 .

[0037] Specifically, the arrangement of the four wing panels 31 allows the four edges of the scratch plate 30 to be stably overlapped with the rectangular frame 10, avoiding the phenomenon of the scratch plate 30 warping due to uneven force applied to the scratch plate 30, and further improving the stability of the scratch plate 30 on the rectangular frame 10.

[0038] Optionally, when used in conjunction with the above-mentioned magnets 33 , the magnets 33 may be installed on only two of the wing plates 31 , wherein the two wing plates 31 are arranged opposite to each other, thereby reducing costs.

[0039] In some embodiments, see Figure 1 、 Figure 3 and Figure 4 The scratch holes 32 are arranged in a plurality of groups at intervals perpendicular to the extending direction thereof, and each group of scratch holes 32 is arranged in a plurality of groups at intervals perpendicular to the extending direction thereof.

[0040] Specifically, each group of scratch holes 32 is located above the culture wells 2 of the corresponding row of cell culture plates 1 , and the cells in the culture wells 2 of the corresponding row can be scratched together through the group of scratch holes 32 , thereby improving the convenience of scratching.

[0041] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A tumor cell scratch assisting device, characterized in that: include: A rectangular frame is used to be sleeved on the periphery of the cell culture plate, and two opposite side walls of the rectangular frame are respectively provided with a slot extending upward to the upper end surface of the rectangular frame; an elastic limiting member, disposed on a side of the rectangular frame and extending through the rectangular frame, wherein the elastic limiting member can elastically abut against a side wall of the cell culture plate to lock the position of the rectangular frame; as well as The scratch plate is horizontally arranged in the rectangular frame, and the opposite side edges of the scratch plate are provided with wing plates corresponding to the two card slots. The scratch plate is penetrated by a horizontally extending scratch hole for being arranged opposite to the culture hole of the cell culture plate.

2. The tumor cell scratching assisting device according to claim 1, characterized in that: A mounting seat is provided on the outside of the rectangular frame. The mounting seat has a mounting cavity that passes through from top to bottom. The elastic limiting member includes: a limiting post, which passes through the mounting seat and the side of the rectangular frame and extends into the rectangular frame, and is slidably engaged with the mounting seat and the rectangular frame, wherein a limiting boss protruding toward the periphery is provided in the middle of the limiting post, and the limiting boss is located in the mounting cavity; and The elastic member is sleeved on the outer periphery of the limiting column and is located between the limiting boss and the inner wall of the mounting cavity, and is used for elastically pushing the limiting column to abut against the side wall of the cell culture plate to lock the position of the rectangular frame.

3. The tumor cell scratching auxiliary device according to claim 2, characterized in that: The outer end of the limiting column is provided with a force application platform protruding toward the outer periphery.

4. The tumor cell scratching assisting device according to claim 3, characterized in that: The inner end of the limiting column is connected to a strip plate for abutting and cooperating with the side wall of the cell culture plate.

5. The tumor cell scratching assisting device according to claim 1, wherein: An iron sheet is embedded on the inner bottom wall of the slot, and a magnet for adsorbing the iron sheet is embedded on the bottom surface of the wing plate.

6. The tumor cell scratching assisting device according to claim 1, wherein: There are four card slots, which are arranged in a one-to-one correspondence with the four side walls of the rectangular frame; there are four wing plates, which are arranged in a one-to-one correspondence with the four card slots.

7. The tumor cell scratching assisting device according to claim 1, wherein: The scratch holes are arranged in a plurality of groups at intervals along a direction perpendicular to the extending direction thereof, and each group of the scratch holes is provided with a plurality of holes at intervals along a direction perpendicular to the extending direction thereof.

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