Reaction kettle applied to nucleoside monomer fluorescent dyeing

Abstract

The reaction kettle comprises a reaction kettle tank body, a cover body is arranged at the top of the reaction kettle tank body, a stirring assembly is arranged in the middle of the cover body, a feeding pipe is arranged on one side of the stirring assembly, a flow valve is arranged at the bottom of the feeding pipe, a feeding port is formed in the middle of the cover body, and a discharging port is formed in the middle of the cover body. The feeding device comprises a feeding pipe and a feeding port, the feeding port is communicated with the feeding pipe, the bottom of the feeding port is fixedly connected with two springs, the bottoms of the springs are fixedly connected with a baffle, and the top of the baffle is fixedly connected with a conical block. The reaction gas in the reaction kettle tank body is prevented from flowing back to the feeding hole and polluting the raw materials along the feeding hole.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological preparation technical field especially is applied to nucleoside monomer fluorescence dyeing's reaction kettle. BACKGROUND

[0002] Nucleoside monomer, short for "nucleoside", is a general term for glycosides, which is a glycoside compound combined by base (purine or pyrimidine) and pentose (ribose or deoxyribose). Because of the different bases, it can be divided into purine nucleosides (such as adenosine, guanosine and inosine) and pyrimidine nucleosides (such as cytidine, uridine and deoxythymidine); because of the different sugar content, it can be divided into ribonucleosides and deoxyribonucleosides. Fluorescence dyeing is a phenomenon that fluorescence dye combines with specific molecules (such as nucleoside monomers) and emits fluorescence under the excitation of specific wavelength light. This technology can be used for labeling and detecting biomolecules such as DNA, RNA, etc.

[0003] Reaction kettle is a comprehensive reaction container. According to the design of reaction kettle structure function and configuration accessory, the starting feeding-reaction-discharging can complete the pre-set reaction steps with high automation degree. The important parameters such as temperature, pressure, mechanical control (stirring, air blowing, etc.), reactant / product concentration in the reaction process are strictly regulated. Its structure is generally composed of kettle body, transmission device, stirring device, heating device, cooling device and sealing device.

[0004] When the reaction kettle is reacting, the generated gas will pollute the raw materials being fed, affecting the subsequent use of the raw materials, and also negatively affecting the quality of the products. UTILITY MODEL CONTENT

[0005] Therefore, the technical problem to be solved by the utility model is to overcome the problem that the generated gas will pollute the raw materials being fed in the prior art.

[0006] To solve the above technical problems, the utility model provides a reaction kettle for nucleoside monomer fluorescence dyeing, including reaction kettle jar body, the reaction kettle jar body top is provided with the cover, the cover middle part is provided with stirring component, stirring component one side is provided with feed pipe, the feed pipe bottom is provided with flow valve, the cover middle part is equipped with feed inlet, the feed inlet is connected with feed pipe, the feed inlet bottom is fixedly connected with two springs, spring bottom is fixedly connected with the baffle, the baffle top is fixedly connected with the tapered block, work, first, adjust the temperature and stirring component stirring strength that nucleoside monomer fluorescence dyeing needs, then raw materials are connected with feed pipe, raw materials pass through flow valve and enter feed inlet, initially, feed inlet is in close contact with the baffle, the tapered block is in feed inlet, the baffle is under the pressure of raw materials and moves, spring supports the baffle, after raw materials slide through the tapered block and drop from the baffle to the inside of reaction kettle jar body, spring bounces back with the baffle, so that the baffle resets, in this process, raw materials fill feed inlet, and the gas in the reaction kettle jar body is prevented from polluting the raw materials at the feed pipe through feed inlet; by setting spring and baffle, raw materials press the baffle through its own gravity, the tapered block guides the raw materials, and then enters the reaction kettle jar body, preventing the reaction gas inside the reaction kettle jar body from flowing back to the feed inlet and then polluting the raw materials along the feed inlet.

[0007] In an embodiment of the utility model, the baffle bottom is connected with a breather hose, the breather hose is fixedly connected with the cover, the breather hose penetrates the cover and extends to the outside, a plurality of air holes are formed in the surface of the tapered block, during operation, the breather hose is connected with an air pump, gas enters the inside of the tapered block through the breather hose and is discharged from the air holes, when the baffle resets, the airflow guides the raw materials, reducing the adhesion of the raw materials to the tapered block and causing the raw materials to accumulate at the baffle.

[0008] In an embodiment of the utility model, two fixing frames are fixedly connected to the surface of the baffle, a support ring is fixedly connected to one end of the fixing frame, and the breather hose is slidably connected to the support ring, during operation, the support ring protects the breather hose, avoiding the breather hose from being bent when the cover moves and causing the breather hose to be damaged.

[0009] In an embodiment of the utility model, the stirring component is provided with a support rod on both sides, the support rod is fixedly connected with the cover, a scraper is fixedly connected to the bottom of the support rod, and the scraper is in contact with the inner wall of the reaction kettle jar body, during operation, when the cover is opened and closed, the scraper is in contact with the surface of the reaction kettle jar body, scraping off the residues adhered to the inner wall of the reaction kettle jar body, and the scraper is curved at both ends, which can accelerate the scraping efficiency.

[0010] In an embodiment of the utility model, the middle part of the supporting rod is provided with a water injection hole, one side of the supporting rod is fixedly connected with a water spraying head, the water spraying head is communicated with the water injection hole, and when working, hot water is injected into the water injection hole, the hot water flows along the supporting rod to the water spraying head, the water spraying head sprays hot water into the inside of the reaction kettle tank body to flush the inside of the reaction kettle tank body.

[0011] In an embodiment of the utility model, the outer side of the supporting rod is fixedly connected with a plurality of cooling fins, the cooling fins are provided in the shape of a circular truncated cone, when working, the supporting rod conducts the heat of the hot water, the heat is transferred to the cooling fins, the cooling fins contact the reactants to provide heat for the reaction, and the purpose of uniform heating is achieved.

[0012] In an embodiment of the utility model, the outer side of the conical block is provided with a sealing ring, the sealing ring is fixedly connected with the baffle, when the baffle is attached to the feeding port, the sealing ring is clamped in the inside of the feeding port to fill the gap between the feeding port and the baffle and increase the sealing property.

[0013] In an embodiment of the utility model, the inner side of the supporting ring is provided with a buffer pad, the buffer pad is fixedly connected with the supporting ring, when working, the buffer pad insulates the direct contact between the supporting ring and the air hose to reduce the damage of the supporting ring to the air hose.

[0014] The above technical scheme of the utility model has the following advantages compared with the prior art:

[0015] The reaction kettle applied to the fluorescent dyeing of nucleoside monomers has the advantages that the spring and the baffle are arranged, the raw materials are pressed downward to the baffle by the self-gravity, the conical block guides the raw materials to enter the inside of the reaction kettle tank body, the backflow of the reaction gas in the inside of the reaction kettle tank body to the feeding port is insulated, and the raw materials are polluted along the feeding port.

[0016] The reaction kettle applied to the fluorescent dyeing of nucleoside monomers has the advantages that the air hose is connected to the air pump, the gas enters the inside of the conical block through the air hose, and is discharged from the air hole, when the baffle is reset, the airflow fills the gap in the feeding port instead of the raw materials, the backflow of the reaction gas to the feeding port is blocked, and the pollution of the raw materials by the reaction gas is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to make the content of the utility model more easily and clearly understood, the utility model is further described in detail below according to the specific embodiments of the utility model and in combination with the drawings.

[0018] Fig. 1 is the perspective view of the utility model;

[0019] Fig. 2 is the structural view of the reaction kettle tank body of the utility model;

[0020] Fig. 3 is a structural diagram of the feeding inlet of the utility model;

[0021] Fig. 4 is a structural diagram of the scraper of the utility model;

[0022] Fig. 5 is a structural diagram of the supporting ring of the utility model;

[0023] The description of the drawings of the specification: 1, the reaction kettle body; 101, stirring assembly; 102, feed pipe; 103, flow valve; 11, cover; 12, feeding inlet; 13, spring; 14, baffle; 15, conical block; 2, breather hose; 21, air hole; 3, fixing frame; 31, supporting ring; 4, supporting rod; 41, scraper; 5, water injection hole; 51, water jet head; 6, cooling fin; 7, sealing ring; 8, buffer pad. PREFERRED EMBODIMENT

[0024] The utility model will be further described below in combination with the drawings and specific embodiments, so that the person skilled in the art can better understand the utility model and can be implemented, but the embodiment is not as the limitation of the utility model.

[0025] Refer to Figs. 1 to 3 The utility model discloses a reaction kettle for nucleoside monomer fluorescence dyeing, including reaction kettle body 1, the reaction kettle body 1 top is provided with cover 11, the cover 11 middle part is provided with stirring assembly 101, stirring assembly 101 one side is provided with feed pipe 102, the feed pipe 102 bottom is provided with flow valve 103, the cover 11 middle part is set up with feeding inlet 12, the feeding inlet 12 with feed pipe 102 intercommunication, the feeding inlet 12 bottom is fixedly connected with two springs 13, the spring 13 bottom is fixedly connected with baffle 14, baffle 14 top is fixedly connected with conical block 15, first, adjust the temperature and the force of stirring assembly 101 stirring that nucleoside monomer fluorescence dyeing needs, then raw materials are connected with feed pipe 102, raw materials pass through flow valve 103 and enter the feeding inlet 12, initially, the feeding inlet 12 is in close contact with baffle 14, conical block 15 is in the feeding inlet 12, baffle 14 is moved under the pressure of raw materials, spring 13 supports baffle 14, after raw materials slide through conical block 15 and drop from baffle 14 to the inside of reaction kettle body 1, spring 13 makes baffle 14 reset with baffle 14, in this process, raw materials fill the feeding inlet 12, and the gas in the reaction kettle body 1 is prevented from polluting the raw materials at the feed pipe 102 through the feeding inlet 12, by setting spring 13 and baffle 14, raw materials are pressed baffle 14 through self weight, conical block 15 guides raw materials, and then enters the reaction kettle body 1, and the reaction gas in the reaction kettle body 1 is prevented from flowing back to the feeding inlet 12, and then polluting the raw materials along the feeding inlet 12.

[0026] Referring to Figs. 1 to 3 As shown in the figure, the bottom of the baffle 14 is communicated with a ventilation hose 2, the ventilation hose 2 is fixedly connected with the cover body 11, the ventilation hose 2 penetrates through the cover body 11 and extends to the outside, a plurality of air holes 21 are arranged on the surface of the tapered block 15, the ventilation hose 2 is connected with an air pump, and gas enters the inside of the tapered block 15 through the ventilation hose 2 and is discharged from the air holes 21. When the baffle 14 is reset, the airflow can dredge the raw materials, so as to reduce the attachment of the raw materials on the tapered block 15 and cause the raw materials to accumulate at the baffle 14.

[0027] Referring to Figs. 1 to 5 As shown in the figure, the surface of the baffle 14 is fixedly connected with two fixed frames 3, one end of the fixed frame 3 is fixedly connected with a support ring 31, and the ventilation hose 2 is slidably connected in the support ring 31. The support ring 31 protects the ventilation hose 2, avoids the bending of the ventilation hose 2 when the cover body 11 moves, and further causes the damage of the ventilation hose 2.

[0028] Referring to Figs. 1 to 4 As shown in the figure, the both sides of the stirring assembly 101 are provided with support rods 4, the support rods 4 are fixedly connected with the cover body 11, the bottom of the support rod 4 is fixedly connected with a scraper 41, the scraper 41 is in contact with the inner wall of the reaction kettle body 1, and the cover body 11 is opened and closed. The scraper 41 is in contact with the surface of the reaction kettle body 1, the residues attached to the inner wall of the reaction kettle body 1 are scraped off, and the scraper 41 is provided in a bent manner at both ends, so that the scraping efficiency can be improved.

[0029] Referring to Figs. 1 to 4 As shown in the figure, the middle part of the support rod 4 is provided with a water injection hole 5, one side of the support rod 4 is fixedly connected with a water spraying head 51, the water spraying head 51 is communicated with the water injection hole 5, hot water is injected into the water injection hole 5, the hot water flows along the support rod 4 to the water spraying head 51, and the water spraying head 51 sprays hot water into the inside of the reaction kettle body 1 to flush the inside of the reaction kettle body 1.

[0030] Referring to Figs. 1 to 4 As shown in the figure, a plurality of heat dissipation fins 6 are fixedly connected to the outer side of the support rod 4, the heat dissipation fins 6 are provided in a circular truncated cone shape, the support rod 4 conducts the heat of the hot water, the heat is transferred to the heat dissipation fins 6, the heat dissipation fins 6 are in contact with the reactants to provide heat for the reaction, and the purpose of uniform heating is achieved.

[0031] Referring to Fig. 3 As shown in the figure, a sealing ring 7 is arranged on the outer side of the tapered block 15, the sealing ring 7 is fixedly connected with the baffle 14, and when the baffle 14 is attached to the feed inlet 12, the sealing ring 7 is clamped in the inside of the feed inlet 12 to fill the gap between the feed inlet 12 and the baffle 14 and increase the sealing property.

[0032] Referring to Fig. 5As shown, the inside of the support ring 31 is provided with a buffer pad 8, the buffer pad 8 is fixedly connected with the support ring 31, the buffer pad 8 isolates the support ring 31 from directly contacting with the breather hose 2, and reduces the damage of the support ring 31 to the breather hose 2.

[0033] Working principle: first, adjust the temperature and stirring intensity of the nucleotide fluorescent dyeing, then connect the raw material with the feeding pipe 102, the raw material enters the feeding port 12 through the flow valve 103, at the beginning, the feeding port 12 is in close contact with the baffle 14, the conical block 15 is in the feeding port 12, the baffle 14 is moved by the pressure of the raw material, the spring 13 supports the baffle 14, after the raw material slides through the conical block 15 and falls from the baffle 14 to the inside of the reaction kettle body 1, the spring 13 rebounds with the baffle 14, so that the baffle 14 resets, in this process, the raw material fills the feeding port 12, and the gas in the reaction kettle body 1 is prevented from polluting the raw material at the feeding pipe 102 through the feeding port 12; by setting the spring 13 and the baffle 14, the raw material presses the baffle 14 by its own gravity, the conical block 15 guides the raw material to enter the reaction kettle body 1, and the reaction gas in the reaction kettle body 1 is prevented from flowing back to the feeding port 12 to pollute the raw material along the feeding port 12, the breather hose 2 is connected with the air pump, the gas enters the conical block 15 through the breather hose 2 and is discharged from the air hole 21, the gas flow guides the raw material, reduces the attachment of the raw material on the conical block 15, causes the raw material to accumulate at the baffle 14, the support ring 31 protects the breather hose 2 from being bent when the cover body 11 moves, thereby preventing the breather hose 2 from being damaged, when the cover body 11 is opened and closed, the scraper 41 contacts the surface of the reaction kettle body 1, scrapes off the residues attached to the inner wall of the reaction kettle body 1, the scraper 41 is bent at both ends, which can speed up the scraping efficiency, hot water is introduced into the water injection hole 5, the hot water flows to the water spraying head 51 along the support rod 4, the water spraying head 51 sprays hot water into the inside of the reaction kettle body 1 to flush the inside of the reaction kettle body 1, the support rod 4 conducts the heat of the hot water, the heat is transferred to the heat dissipation fin 6, and the heat is contacted with the reactant to provide heat for the reaction, so that the purpose of uniform heating is achieved, when the baffle 14 is attached to the feeding port 12, the sealing ring 7 is clamped in the inside of the feeding port 12 to fill the gap between the feeding port 12 and the baffle 14, and the sealing performance is increased, the buffer pad 8 isolates the support ring 31 from directly contacting with the breather hose 2, and reduces the damage of the support ring 31 to the breather hose 2.

[0034] Obviously, the above embodiments are only examples for clearly illustrating, and are not limited to the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments cannot be exhausted, and the obvious changes or variations derived therefrom are still within the protection scope of the utility model.

Claims

1. A reactor for fluorescent staining of nucleoside monomers, comprising a reactor tank (1), characterized in that: The reactor tank body (1) is provided with a cover (11) on the top, a stirring assembly (101) is provided in the middle of the cover (11), a feed pipe (102) is provided on one side of the stirring assembly (101), a flow valve (103) is provided at the bottom of the feed pipe (102), a feed port (12) is provided in the middle of the cover (11), the feed port (12) is communicated with the feed pipe (102), two springs (13) are fixedly connected to the bottom of the feed port (12), a baffle (14) is fixedly connected to the bottom of the spring (13), and a conical block (15) is fixedly connected to the top of the baffle (14).

2. The reactor for fluorescent staining of nucleoside monomers according to claim 1, characterized in that: The bottom of the baffle (14) is connected to a ventilation hose (2), which is fixedly connected to the cover (11). The ventilation hose (2) passes through the cover (11) and extends to the outside. A plurality of air holes (21) are provided on the surface of the conical block (15).

3. The reactor for fluorescent staining of nucleoside monomers according to claim 2, characterized in that: Two fixing frames (3) are fixedly connected to the surface of the baffle (14), one end of the fixing frame (3) is fixedly connected to a support ring (31), and a ventilation hose (2) is slidably connected inside the support ring (31).

4. The reactor for fluorescent staining of nucleoside monomers according to claim 3, characterized in that: Support rods (4) are provided on both sides of the stirring assembly (101), the support rods (4) are fixedly connected to the cover body (11), and a scraper (41) is fixedly connected to the bottom of the support rod (4), and the scraper (41) contacts the inner wall of the reactor tank body (1).

5. The reactor for fluorescent staining of nucleoside monomers according to claim 4, characterized in that: A water injection hole (5) is provided in the middle of the support rod (4), and a water spray head (51) is fixedly connected to one side of the support rod (4), and the water spray head (51) is communicated with the water injection hole (5).

6. The reactor for fluorescent staining of nucleoside monomers according to claim 5, characterized in that: A plurality of heat sinks (6) are fixedly connected to the outside of the support rod (4), and the shape of the heat sink (6) is set to be a truncated cone.

7. The reactor for fluorescent staining of nucleoside monomers according to claim 6, characterized in that: A sealing ring (7) is provided on the outside of the conical block (15), and the sealing ring (7) is fixedly connected to the baffle (14).

8. The reactor for fluorescent staining of nucleoside monomers according to claim 7, characterized in that: A buffer pad (8) is provided on the inner side of the support ring (31), and the buffer pad (8) is fixedly connected to the support ring (31).

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