Automatic purification equipment

Abstract

The utility model provides automatic purification equipment, and belongs to the technical field of biotechnology equipment. According to the automatic purification equipment provided by the utility model, the automatic purification equipment comprises a storage and discharge assembly, a metering assembly, an injection assembly and a control unit, the metering assembly is communicated with the injection assembly, and the metering assembly and the injection assembly are respectively and electrically connected with the control unit; the control unit controls the metering assembly to input a reagent into the injection assembly, meanwhile, the injection assembly injects the reagent into the storage and discharge assembly, when the reagent is completed, the control unit controls the injection assembly and the metering assembly to stop injection of the reagent, the whole process is sequentially carried out in the equipment, manual intervention is reduced, and the injection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biotechnology equipment, especially relates to an automatic purification equipment. BACKGROUND

[0002] In biological gene experiments, when nucleic acids, proteins and other substances are extracted and purified in reaction containers, liquid addition, standing and filtration and other process flows need to be carried out according to process requirements, and the above-mentioned flows need to be repeatedly carried out.

[0003] In the related art, when the liquid addition, standing and filtration flows are carried out, an operator first needs to place a synthesis plate on a reagent injection device, manually use the reagent injection device to inject reagents, then take the synthesis plate from the reagent injection device and place it on a liquid extraction tank connected with a filtration device, and after standing is completed, the operator starts the filtration device to extract waste liquid. In the experiment, manual intervention is required between the liquid addition, standing and filtration process flows, and time is required for switching between the flows, resulting in low efficiency of nucleic acid and protein extraction and purification. SUMMARY

[0004] The present application aims to at least solve the technical problem of low efficiency caused by too much manual intervention in the extraction and purification of nucleic acids, proteins and other substances. To this end, the present application provides an automatic purification equipment.

[0005] The utility model provides an automatic purification equipment, which comprises:

[0006] a metering assembly;

[0007] an injection assembly in communication with the metering assembly;

[0008] a storage and discharge assembly disposed close to the injection assembly to enable the injection assembly to inject reagents toward the storage and discharge assembly; and

[0009] a control unit electrically connected to the metering assembly and the injection assembly to enable the metering assembly and the injection assembly to start and stop.

[0010] When reagents are injected, the control unit controls the metering assembly to input reagents to the injection assembly, and at the same time, the injection assembly injects the reagents into the storage and discharge assembly. When the reagent injection is completed, the control unit controls the injection assembly and the metering assembly to stop injecting reagents. The entire process is sequentially performed inside the equipment, reducing manual intervention and improving injection efficiency.

[0011] Further, the metering assembly comprises an injection pump, the injection assembly comprises a driving member and an injection member connected to each other, the injection pump and the driving member are electrically connected to the control unit respectively, and the injection pump and the injection member are in communication.

[0012] In order to improve the injection efficiency, in some embodiments, the number of injection pumps is multiple, and the multiple injection pumps are sequentially and spacedly arranged, the injection assembly has multiple injection needles sequentially and spacedly arranged, the multiple injection pumps are respectively communicated with the multiple injection needles, and the multiple injection pumps respectively store different reagents, so that the operator can inject different reagents into the synthesis column without replacing the injection needle, and the injection efficiency is improved.

[0013] Further, the injection pump has multiple liquid storage cylinders and a liquid pushing rod sequentially and spacedly arranged, the multiple liquid storage cylinders are respectively communicated with the multiple injection needles, the liquid pushing rod is movably arranged in the liquid storage cylinder, and the liquid pushing rod has a liquid pushing end, so that the liquid pushing rod can push the reagent from the liquid storage cylinder to the injection needle, wherein the multiple liquid storage cylinders are respectively communicated with the multiple injection needles, so that when the injection needle injects the reagent into the synthesis plate, the multiple injection needles can simultaneously inject the reagent into the multiple synthesis columns, and the injection efficiency is improved.

[0014] In some embodiments, the injection pump is further provided with a first servo motor, a guide sliding block and a screw rod in transmission connection with the first servo motor, the first servo motor is electrically connected with the control unit, the guide sliding block is in threaded connection with the screw rod, and the liquid storage cylinder is connected with the guide sliding block.

[0015] In some embodiments, the driving member includes a synchronous belt, a second servo motor and a synchronous wheel, the second servo motor is connected with the synchronous wheel, the synchronous belt is connected with the synchronous wheel, the second servo motor is electrically connected with the control unit, and the injection needle is arranged at one end of the synchronous belt.

[0016] In some embodiments, the storage and discharge assembly includes multiple synthesis plates, a liquid pumping groove and a liquid discharging electromagnetic valve, the synthesis plates are arranged in the liquid pumping groove, and the liquid discharging electromagnetic valve is arranged at the bottom of the liquid pumping groove and communicated with the liquid pumping groove.

[0017] In some embodiments, the storage and discharge assembly further includes a waste liquid barrel and a direct discharge electromagnetic valve communicated with the waste liquid barrel, the liquid pumping groove is further provided with multiple direct discharge cavities sequentially and spacedly arranged, the multiple direct discharge cavities are all communicated with the direct discharge electromagnetic valve, and the direct discharge electromagnetic valve is electrically connected with the control unit.

[0018] Further, the automatic purification equipment further includes a constant pressure electromagnetic valve communicated with the liquid pumping groove, the constant pressure electromagnetic valve is electrically connected with the control unit, when the reagent injection is completed and the synthesis plate needs to be removed, the control unit controls the constant pressure electromagnetic valve to be opened, after the constant pressure for a specified time, the pressure in the liquid pumping cavity returns to normal, at this time, the synthesis plate is relatively easy to be removed.

[0019] In some embodiments, the control unit is arranged at the bottom of the plurality of injection pumps arranged on the upper side of the liquid extraction tank, so that the reagent can be transferred by gravity when the liquid transfer cylinder is transferred to the injection needle.

[0020] In the utility model, the automatic purification equipment includes a storage and discharge assembly, a metering assembly, an injection assembly and a control unit, the metering assembly and the injection assembly are communicated, and the metering assembly and the injection assembly are electrically connected with the control unit; when reagent injection is performed, the control unit controls the metering assembly to input reagent to the injection assembly; at the same time, the injection assembly injects the reagent into the storage and discharge assembly; when the reagent injection is completed, the control unit controls the injection assembly and the metering assembly to stop the reagent injection; the whole process is sequentially performed in the equipment, manual intervention is reduced, and the injection efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical scheme in the utility model, the following will briefly introduce the drawings needed to be used in the embodiment description, and obviously, the drawings in the following description are some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the drawings.

[0022] Figure 1 It is a structure schematic view of the automatic purification equipment of the utility model;

[0023] Figure 2 It is a structure schematic view of the injection pump of the automatic purification equipment of the utility model;

[0024] Figure 3 It is a top structure schematic view of the automatic purification equipment of the utility model;

[0025] Figure 4 It is a side structure schematic view of the automatic purification equipment of the utility model;

[0026] Figure 5 It is a front structure schematic view of the automatic purification equipment of the utility model;

[0027] Figure 6 It is a structure schematic view of the injection assembly of the automatic purification equipment of the utility model.

[0028] Reference signs:

[0029] 10-storage and discharge assembly,

[0030] 100-combination plate, 110-combination column,

[0031] 200-liquid extraction tank, 210-liquid extraction cavity, 220-direct discharge cavity, 230-supporting leg

[0032] 300-drain solenoid valve,

[0033] 400-waste liquid barrel,

[0034] 500-vacuum pump,

[0035] 600-measuring assembly, 610-reagent bottle, 620-syringe pump, 621-liquid storage cylinder, 622-liquid pushing rod, 622a-liquid pushing end, 623-first servo motor, 624-screw rod, 625-guiding sliding block, 626-connecting block, 627-mounting plate, 627a-moving groove,

[0036] 700-pressure detecting member,

[0037] 800-injection assembly, 810-driving member, 811-moving end, 812-synchronous belt, 813-second servo motor, 814-synchronous wheel, 820-injection member,

[0038] 900-control unit,

[0039] 1000-direct drain solenoid valve,

[0040] 1100-constant pressure solenoid valve. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0042] It should be noted that all directional indications in the embodiments of the present application are only used to explain the relative position relationship, movement condition and the like between components in a certain posture, and if the certain posture changes, the directional indications also change accordingly.

[0043] In the present application, unless otherwise explicitly specified and limited, the terms "connection", "fixation" and the like should be understood in a broad sense, for example, "fixation" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through an intermediate medium; can be internal connection of two elements or interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0044] In addition, the description such as "first", "second" in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second" can be explicitly or implicitly included at least one of the features. In addition, the technical solutions of various embodiments can be combined with each other, but it must be based on the realization of the ordinary skilled in the art, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, nor in the protection scope required by the present application.

[0045] The present application will be described below in conjunction with the drawings and specific embodiments:

[0046] Please refer to Figures 1-6 The automatic purification equipment provided by the present application mainly combines the reagent injection, standing, filtration and other process flows of nucleic acid and protein (for convenience of description, the reactant is used instead hereinafter) into a unified whole through the connection and cooperation between various components.

[0047] Among them, the automatic purification equipment further comprises a base, which is used as the installation basis of the automatic purification equipment. The base is made of metal materials such as stainless steel. Stainless steel has the properties of anti-corrosion, easy cleaning and not easy to attach dust, and has good metal properties, so that the base is easy to clean and has a long service life. At the same time, the stainless steel material is easy to obtain, which reduces the manufacturing difficulty. Among them, the base can adopt shapes such as rectangle and circle. In the present embodiment, the base adopts a rectangular shape, so that the automatic purification equipment is placed on the ground stably, and the manufacturing precision and difficulty are reduced.

[0048] Among them, the storage and discharge assembly comprises a synthesis plate 100, a liquid extraction tank 200, a liquid discharge electromagnetic valve 300, a waste liquid bucket 400 and a vacuum pump 500.

[0049] Specifically, the synthesis plate 100 has a plurality of synthesis columns 110. Among them, the synthesis columns 110 decrease in size from the liquid inlet to the liquid outlet, so that the reagent stored in the synthesis columns 110 has a large pressure itself when the reagent is filtered, which is beneficial to the filtration of the reagent.

[0050] Among them, the synthesis plate 100 itself can adopt polygons such as rectangle, pentagon and circle. Specifically, it can be selected according to the size or shape of the liquid extraction cavity 210. In the present embodiment, the synthesis plate 100 is selected to be rectangular. Specifically, the synthesis plate 100 has a rectangular plate body, and a plurality of synthesis columns 110 are arranged in the plate body in the first direction. Among them, the plurality of synthesis columns 110 are arranged in a rectangular array by being arranged longitudinally and transversely, so that the synthesis plate 100 can accommodate more reagents.

[0051] The liquid extraction tank 200 is arranged on the base. Specifically, the liquid extraction tank 200 can adopt a rectangular body, a cylindrical body, etc. In the embodiment, the liquid extraction tank 200 adopts a rectangular body, so that the manufacturing precision and difficulty of the liquid extraction tank 200 are reduced. The liquid extraction tank 200 has a liquid extraction cavity 210, which is matched with the shape and size of the synthetic plate 100. When the reagent is filled, the synthetic plate 100 is placed at the top opening of the liquid extraction cavity 210, and the synthetic column 110 is located in the liquid extraction cavity 210, so that the liquid extraction cavity 210 forms a sealed chamber.

[0052] The bottom of the liquid extraction tank 200 is provided with a plurality of supporting legs, which are arranged in a circumferential direction at intervals along the bottom surface of the liquid extraction tank 200. The liquid extraction tank 200 is stably arranged on the base and is not easy to be inclined or shaken, so that the synthetic plate 100 is not easily separated or the injection component 820 is not easily misaligned with the synthetic plate 100.

[0053] The liquid discharge electromagnetic valve 300 is arranged at the bottom of the liquid extraction tank 200 and is communicated with the waste liquid barrel 400 through a pipeline. The waste liquid barrel 400 is communicated with the vacuum pump 500. Specifically, the liquid discharge electromagnetic valve 300 can adopt a cylindrical body or a rectangular body structure. In the application, the liquid discharge electromagnetic valve 300 adopts a cylindrical structure. The liquid discharge electromagnetic valve 300 is communicated with the liquid extraction cavity 210 through a pipeline, so that the reagent can be discharged through the liquid discharge electromagnetic valve 300. The liquid discharge electromagnetic valve 300 and the vacuum pump 500 are electrically connected with the control unit 900. When the reagent needs to be discharged, the control unit 900 controls the vacuum pump 500 to be started, so that the waste liquid barrel 400 and the pipeline between the waste liquid barrel 400 and the liquid discharge electromagnetic valve are vacuumized, so that there is a negative pressure in the pipeline. Then, the control unit 900 controls the liquid discharge electromagnetic valve 300 to be started. At this time, there is a pressure difference between the liquid extraction cavity 210 and the pipeline between the waste liquid barrel 400 and the liquid discharge electromagnetic valve, so that the reagent in the synthetic column 110 in the liquid extraction cavity 210 is extracted to the waste liquid barrel 400 under the action of the pressure difference. When the reagent extraction is completed, the control unit 900 controls the liquid discharge electromagnetic valve 300 and the vacuum pump 500 to be closed.

[0054] The metering assembly 600 has an injection pump 620, which is communicated with a reagent bottle 610 containing a reagent and is arranged on the base. The injection pump 620 is electrically connected with the control unit 900.

[0055] The injection assembly 800 has a driving member 810 electrically connected with the control unit 900 and an injection needle 820 connected with a moving end 811 of the driving member 810, wherein the injection needle 820 is in communication with the injection pump 620, and the moving end 811 is located on the upper side of the synthesis plate 100. When the reagent injection is performed, the driving member 810 drives the injection needle to move in the first direction under the control of the control unit 900. When the injection needle 820 moves to the corresponding synthesis column 110, the control unit 900 controls the driving member 810 to stop, and at the same time, the control unit 900 controls the injection pump 620 to output the reagent to the injection needle 820, so that the injection needle 820 injects the reagent into the synthesis column 110 of the synthesis plate 100. When the reagent injection of the synthesis column 110 is completed, the control unit 900 controls the injection pump 620 to stop outputting the reagent, and at the same time, the control unit 900 controls the driving member 810 to drive the injection needle 820 to continue moving in the first direction to the next synthesis column 110. The above process is repeated to complete the reagent injection of the synthesis column 110.

[0056] The pressure detection member 700 is arranged in the pipeline between the waste liquid tank 400 and the liquid discharge electromagnetic valve 300. When the synthesis column 110 completes the reagent injection and stands for a specified time, the control unit 900 controls the vacuum pump 500 to start, and performs the vacuumizing treatment on the pipeline between the waste liquid tank 400 and the liquid discharge electromagnetic valve 300. At this time, the pressure detection member 700 detects the pressure in the pipeline. If the pressure in the pipeline reaches the preset value, the pressure detection member 700 feeds back the information to the control unit 900. At this time, the control unit 900 controls the vacuum pump 500 to stop running, and at the same time, the control unit 900 controls the liquid discharge electromagnetic valve 300 to open, and performs the suction filtration of the reagent in the synthesis column 110. When the suction filtration is completed, the pressure in the pipeline changes. When the pressure changes to the preset value, the pressure detection member 700 feeds back the signal to the control unit 900, and the control unit 900 controls the liquid discharge electromagnetic valve 300 to close.

[0057] The automatic purification device further comprises a control panel electrically connected with the control unit 900, which is used to control the running and stopping of the device. When the device fails, the operator can stop the device at any time to avoid further damage to the device. At the same time, the control panel can be used to set the detection range of the pressure detection member 700, the liquid pushing interval of the liquid pushing rod 622 and other data to meet different process requirements. The control panel displays the pressure value of the pipeline between the waste liquid tank 400 and the liquid discharge electromagnetic valve 300 in real time.

[0058] In some embodiments, please refer to Figure 1 and Figure 5, the number of injection pumps 620 can be multiple, and the multiple injection pumps 620 are sequentially and spacedly arranged on the base, the number of injection needles 820 is multiple, and the multiple injection needles 820 are sequentially and spacedly arranged along the first direction, wherein the multiple injection needles 820 are respectively communicated with the multiple injection pumps 620, when different reagents need to be injected, the multiple injection pumps 620 respectively store different reagents, so that different reagents can be injected into the synthesis column 110 without replacing the injection needle 820, and the injection efficiency is improved.

[0059] In a more perfect scheme, please refer to Figure 1 、 Figure 5 and Figure 6 , the injection pump 620 has multiple sequentially and spacedly arranged liquid storage cylinders 621 and liquid pushing rods 622, and the injection needle 820 has multiple sequentially and spacedly arranged injection needles, and the injection needles are sequentially and spacedly arranged along the direction perpendicular to the first direction, wherein the multiple liquid storage cylinders 621 are respectively communicated with the multiple injection needles, so that when the synthesis plate 100 is injected, multiple synthesis columns 110 can be simultaneously injected with reagents, and the injection efficiency is improved.

[0060] Among them, the liquid pushing rod 622 is arranged in the liquid storage cylinder 621 along the axial direction of the liquid storage cylinder 621, wherein the liquid pushing rod 622 has a liquid pushing end 622a, the liquid pushing end 622a can drive the liquid pushing rod 622 to reciprocate in the liquid storage cylinder 621 along the axial direction of the liquid storage cylinder 621, and then push the reagent in the liquid storage cylinder 621 to the injection needle.

[0061] The injection pump 620 is also provided with a first servo motor 623, a lead screw 624, a guide sliding block 625, a connecting block 626 and a mounting plate 627 with a moving groove 627a, wherein the first servo motor 623 is arranged on the both side surfaces of the mounting plate 627 opposite to the liquid storage cylinder 621, specifically, the axial direction of the first servo motor 623 is consistent with the direction of gravity, and the first servo motor 623 is in transmission connection with the lead screw 624, so as to drive the lead screw 624 to rotate by the first servo motor 623.

[0062] The guide sliding block 625 is threadedly connected with the screw rod 624, and the ends of the plurality of liquid pushing rods 622 away from the liquid pushing end 622a are fixedly connected with the connecting block 626, at this time, the connecting block 626 is horizontal, wherein the guide sliding block 625 and the connecting block 626 are connected with the moving groove 627a, and the moving groove 627a is arranged in the vertical direction, so that the guide sliding block 625 and the connecting block 626 can only reciprocate in the vertical direction. Specifically, the first servo motor 623 is electrically connected with the control unit 900, when reagent injection is performed, the operator runs the equipment through the control panel, at this time, when the liquid storage cylinder 621 has completed reagent storage, reagent injection needs to be performed, the control unit 900 controls the first servo motor 623 to run, at the same time, the screw rod 624 is driven to rotate, at this time, the screw rod 624 rotates relative to the guide sliding block 625, and then drives the guide sliding block 625 and the connecting block 626 to move along the moving groove 627a, the connecting block 626 drives the liquid pushing rod 622 to move, so that the reagent in the liquid storage cylinder 621 is transported to the injection needle, when the injection is completed, the control unit 900 controls the first servo motor 623 to rotate in the reverse direction, and then the liquid pushing rod 622 moves in the reverse direction along the moving groove 627a, so that the reagent can be input into the liquid storage cylinder 621 again.

[0063] The driving member 810 includes a synchronous belt 812, a second servo motor 813 and a synchronous wheel 814, the motor shaft of the second servo motor 813 is in transmission connection with the synchronous wheel 814, wherein the synchronous belt 812 is connected with the synchronous wheel 814, and the second servo motor 813 is electrically connected with the control unit 900, when reagent injection is performed, the control unit 900 controls the second servo motor 813 to drive the synchronous wheel 814 to rotate, at the same time, the synchronous wheel 814 drives the moving end 811 of the synchronous belt 812 to move in the first direction, so that the injection needle moves in the first direction, and then the injection needle can inject reagent into the synthesis column 110.

[0064] In some embodiments, the number of synthesis plates 100 is multiple, the plurality of synthesis plates 100 are sequentially and spacedly arranged in the first direction, the number of liquid pumping cavities 210 corresponds to the number of synthesis plates 100, the number of liquid discharge electromagnetic valves 300 is multiple, the plurality of liquid discharge electromagnetic valves 300 are respectively communicated with the plurality of liquid pumping cavities 210 and sequentially and spacedly arranged at the bottom of the liquid discharge groove, and the plurality of liquid discharge electromagnetic valves 300 are all communicated with the waste liquid barrel 400 through pipelines, so that in biological experiments, more reactants can be simultaneously injected with reagents, and then the output efficiency of the reactants is improved.

[0065] It should be understood that when the liquid transferring cylinder transports reagent to the injection needle, there may be bubbles in the reagent, and if reagent injection is performed at this time, the injection amount of the reagent may be reduced, which affects the final experimental results.

[0066] To improve the above problems, in some embodiments, the automatic purification device further comprises a direct drainage electromagnetic valve 1000 electrically connected with the control unit 900, the direct drainage electromagnetic valve 1000 is in communication with the waste liquid tank 400 through a pipeline, wherein the pipeline in communication with the direct drainage electromagnetic valve 1000 and the waste liquid tank 400 is oppositely arranged with the pipeline in communication with the drainage electromagnetic valve 300 and the waste liquid tank 400, and both are provided with pressure sensing members.

[0067] Wherein the liquid suction groove 200 is further provided with a plurality of direct drainage cavities 220, the plurality of direct drainage cavities 220 are arranged in pairs with the plurality of liquid suction cavities 210, and the direct drainage cavity 220 located at the front end, specifically, the plurality of direct drainage cavities 220 and the plurality of liquid suction cavities 210 are arranged in sequence, when reagent injection is performed, the injection needle is drained to the direct drainage cavity 220 located at the front end, thereby ensuring that subsequent injection will not appear bubbles, at the same time, the direct drainage cavities 220 are arranged between the liquid suction cavities 210, ensuring that when the injection needle moves between two adjacent synthesis plates 100, the reagent in the injection needle falls to the surface of the drainage groove, so that the drainage groove is contaminated or corroded by the reagent, and according to actual process requirements, one or more direct drainage cavities 220 can be drained to ensure the stability of reagent injection.

[0068] It should be understood that when the synthesis plate 100 is vacuum filtered, the pressure in the liquid suction cavity 210 decreases, so that the synthesis plate 100 is adsorbed on the drainage cavity, at this time, if the synthesis plate 100 is to be removed, only a larger force is used to remove the synthesis plate 100, which is easy to damage the synthesis plate 100, or the pressure in the liquid suction cavity 210 is slowly restored through the small gap of the synthesis column 110 or other structures, and then the synthesis plate 100 is removed, which is low in efficiency.

[0069] To improve the above problems, in some embodiments, the storage and drainage member further comprises a plurality of constant pressure electromagnetic valves 1100 electrically connected with the control unit 900, the plurality of constant pressure electromagnetic valves 1100 are arranged in sequence on the base, wherein the plurality of constant pressure electromagnetic valves 1100 are respectively in communication with the plurality of liquid suction cavities 210, when the reagent injection is completed and the synthesis plate 100 needs to be removed, the control unit 900 controls the constant pressure electromagnetic valve to open, after a specified time of constant pressure, the pressure in the liquid suction cavity 210 returns to normal, at this time, the synthesis plate 100 can be removed.

[0070] Further, the control unit 900 is arranged at the bottom of the plurality of injection pumps 620, so that the height of the bottom end of the injection pump 620 is not lower than the height of the liquid suction groove 200, specifically, the communication position of the injection pump 620 and the injection needle 820 is higher than the synthesis plate 100, so that the reagent can flow to the injection needle under the action of gravity.

[0071] In a more complete scheme, the injection pump 620 further comprises a plurality of liquid inlet electromagnetic valves, wherein the number of the liquid inlet electromagnetic valves corresponds to the number of the rotating liquid cylinders, and the plurality of liquid inlet electromagnetic valves are sequentially and spacedly arranged at the communication positions between the rotating liquid cylinders and the reagent bottles 610, so as to control the input of the reagents and avoid sealing the rotating liquid cylinders and contaminating the reagents in the rotating liquid cylinders by the external environment, thereby affecting the experimental results.

[0072] In implementation, the control unit can adopt an existing controller, control module or control circuit, which will not be illustrated one by one here.

[0073] The working principle of the automatic purification equipment provided by the utility model is as follows:

[0074] In the utility model, when the reagents are injected, the liquid inlet electromagnetic valve is opened, the reagents in the reagent bottles 610 enter the liquid storage cylinders 621, and then the electromagnetic valve is closed.

[0075] The first servo motor 623 drives the liquid pushing rod 622 to make the reagents in the liquid storage cylinders 621 enter the straight drainage cavity 220 through the injection needle, and after the emptying is completed, the first servo motor 623 stops running, the second servo motor 813 is opened, drives the synchronous belt 812 to drive the injection needle to move to the synthesis column 110 in the first direction, and then the second servo motor 813 stops, at this time, the first servo motor 623 is started, drives the liquid pushing rod 622 to move along the moving groove 627a, so as to push the reagents to the injection needle and then inject the synthesis column 110, after the injection is completed, the first servo motor 623 is stopped, the second servo motor 813 drives the injection needle to move to the subsequent synthesis column 110, and then the above process is repeated until the injection of the reagents is completed, the synthesis plate 100 is placed and fixed for a period of time, then the vacuum pump 500 is opened to perform vacuum treatment on the pipeline between the waste liquid barrel 400 and the liquid drainage electromagnetic valve, after the treatment is completed, the vacuum pump 500 is closed, the liquid drainage electromagnetic valve 300 is opened to perform suction filtration of the waste liquid, after the suction filtration is completed, the liquid drainage electromagnetic valve 300 is closed, wherein the first servo motor 623, the second servo motor 813, the vacuum pump 500, the injection pump 620 and the like are controlled by the control unit 900.

[0076] In summary, the automatic purification equipment provided by the utility model combines and connects different structures of the equipment, designs different process flows as a whole, has less manual intervention during equipment operation, switches between different flows quickly, and thus improves the extraction and purification efficiency of the reactants.

[0077] In the description of the specification, reference to "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" means that a particular feature, structure, material, or characteristic being described is included in at least one embodiment or example of the application. The appearances of the phrases "in one embodiment", "in some embodiments", "an example", "a specific example", or "some examples" in various places in the specification are not necessarily referring to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples. Moreover, the terminology used in the specification is for the purpose of describing the particular versions of the application and is not intended to be limiting. The terminology can be standardized by the IEEE or other standards bodies.

Claims

1. An automatic purification device, characterized in that: include: Metering Component (600); an injection assembly (800), communicating with the metering assembly (600); A storage and discharge assembly (10) is arranged close to the injection assembly (800), and the injection assembly (800) injects the reagent toward the storage and discharge assembly (10); and A control unit (900) is electrically connected to the metering component (600) and the injection component (800) to start and stop the metering component (600) and the injection component (800).

2. The automatic purification equipment according to claim 1, characterized in that The metering component (600) includes a syringe pump (620), and the injection component (800) includes a driving member (810) and an injection member (820) connected to each other, wherein the syringe pump (620) and the driving member (810) are electrically connected to the control unit (900) respectively, and the syringe pump (620) and the injection member (820) are in communication.

3. The automatic purification equipment according to claim 2, characterized in that There are multiple injection pumps (620), and the multiple injection pumps (620) are arranged in sequence and at intervals. The injection assembly (800) has multiple injection pieces (820) arranged in sequence and at intervals, wherein the multiple injection pumps (620) are respectively connected to the multiple injection pieces (820).

4. The automatic purification equipment according to claim 3, characterized in that The injection pump (620) comprises a plurality of liquid storage cylinders (621) and liquid pushing rods (622) which are arranged in sequence and spaced apart from each other. The plurality of liquid storage cylinders (621) are respectively connected to the plurality of injection pieces (820). The liquid pushing rod (622) is movably arranged in the liquid storage cylinders (621) and has a liquid pushing end (622a).

5. The automatic purification equipment according to claim 4, characterized in that The injection pump (620) is further provided with a first servo motor (623), a guide slider (625), and a screw rod (624) rotatably connected to the first servo motor (623); the first servo motor (623) is electrically connected to the control unit (900); the guide slider (625) is threadedly connected to the screw rod (624); and the liquid storage cylinder (621) is connected to the guide slider (625).

6. The automatic purification equipment according to claim 2, characterized in that The driving member (810) includes a synchronous belt (812), a second servo motor (813) and a synchronous wheel (814), wherein the second servo motor (813) is connected to the synchronous wheel (814), the synchronous belt (812) is connected to the synchronous wheel (814), the second servo motor (813) is electrically connected to the control unit (900), and the injection member (820) is arranged at one end of the synchronous belt (812).

7. The automatic purification equipment according to claim 6, characterized in that The storage and drainage assembly (10) comprises a plurality of synthetic plates (100), a liquid extraction trough (200), and a liquid drainage solenoid valve (300), wherein the synthetic plates (100) are arranged on the liquid extraction trough (200), and the liquid drainage solenoid valve (300) is arranged on the bottom of the liquid extraction trough (200) and is in communication with the liquid extraction trough (200).

8. The automatic purification equipment according to claim 7, characterized in that The storage and discharge assembly (10) further comprises a waste liquid barrel (400), and a direct discharge solenoid valve (1000) in communication with the waste liquid barrel (400). The liquid extraction tank (200) is further provided with a plurality of direct discharge chambers (220), wherein the plurality of direct discharge chambers (220) are sequentially spaced apart, and the plurality of direct discharge chambers (220) are all in communication with the direct discharge solenoid valve (1000). The direct discharge solenoid valve (1000) is electrically connected to the control unit (900).

9. The automatic purification equipment according to claim 8, characterized in that The storage and drainage assembly (10) further comprises a constant pressure solenoid valve (1100), wherein the constant pressure solenoid valve (1100) is in communication with the liquid extraction tank (200), and the constant pressure solenoid valve (1100) is electrically connected to the control unit (900).

10. The automatic purification equipment according to claim 7, characterized in that The control unit (900) is arranged at the bottom of the plurality of injection pumps (620), and the injection pumps (620) are arranged on the upper side of the liquid extraction tank (200).

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