Micro-sampling mechanism and biochemical detection sampling device

Abstract

The utility model provides a microscale sample injection mechanism and a biochemical detection sample injection device. The microscale sample injection mechanism comprises a mounting frame, a rotary sample conveying assembly, a pushing piece and a guide piece, a tray and a mounting plate are arranged on the mounting frame, and the mounting plate is used for mounting a material moving arm with a sample adding needle; the rotary sample conveying assembly is arranged on the tray and is provided with a material stirring wheel, and the material stirring wheel is used for driving a sample bottle to move in the circumferential direction and pass through the position under the sample adding needle; the pushing piece is connected to the tray, is aligned with the sample adding needle up and down, and is used for upwards pushing the sample bottle positioned under the sample adding needle; the guide piece is vertically connected to the mounting plate and is aligned with the sample adding needle up and down, the lower end of the guide piece is suitable for the top of the sample bottle to be embedded, and the guide piece is provided with a needle guide hole suitable for the needle head of the sample adding needle to penetrate through. According to the micro-sampling mechanism and the biochemical detection sampling device provided by the utility model, the protectiveness of the sampling needle in the sampling process can be improved, and the detection precision is prevented from being influenced by direct contact between a sample and external air.

Description

TECHNICAL FIELD

[0001] The utility model belongs to biochemical detection technical field, concretely relates to a trace sample injection mechanism and biochemical detection sample adding device. BACKGROUND

[0002] In the field of biochemical detection, it is usually necessary to use a sample adding needle to quantitatively transfer a sample, the sample adding needle is installed on a material moving arm of a pipetting device, and considering the space transfer requirement, the material moving arm for installing the sample adding needle needs to have at least X-Y-Z space three-axis displacement freedom. Since the needle diameter of the sample adding needle is very small, especially the sample adding needle used in the case of micro pipetting is even smaller, the sample adding needle is prone to bending deformation or even breaking when the sample adding needle is pierced into a sample bottle to quantitatively suck and discharge the sample, and in order to avoid this situation, the sealing cover of the sample bottle usually needs to be opened in advance, but this will cause the sample to be directly in contact with the air outside, which is very unfavorable to the detection precision of the sample, and therefore it is urgent to improve the sample injection form to avoid the above problems. SUMMARY

[0003] The utility model embodiment provides a trace sample injection mechanism and biochemical detection sample adding device, aims at improving the protection of the sample adding needle in the sample injection process, and avoiding the direct contact of the sample with the air outside to affect the detection precision.

[0004] To achieve the above object, the utility model adopts the technical scheme of: first, a trace sample injection mechanism is provided, comprising:

[0005] A mounting frame is provided with a tray and a mounting plate located above the tray, the mounting plate is used to install a material moving arm, and the material moving arm is used to install a sample adding needle and drive the sample adding needle to vertically ascend and descend;

[0006] A rotary sample feeding assembly is arranged on the tray and has a material stirring wheel, the material stirring wheel is used to drive a sample bottle placed on the tray to move along the circumferential direction thereof and pass through the directly below of the sample adding needle;

[0007] A pushing piece is connected to the tray and vertically aligned with the sample adding needle, and the output end of the pushing piece is used to push the sample bottle located directly below the sample adding needle upward;

[0008] A guiding piece is vertically connected to the mounting plate and vertically aligned with the sample adding needle, the lower end of the guiding piece is adapted to be embedded in the top of the sample bottle, and the guiding piece has a needle guiding hole through which the needle head of the sample adding needle passes; the guiding piece is used to guide the bottle body of the sample bottle when the pushing piece lifts the sample bottle, and guide the needle head to be inserted into the sample bottle.

[0009] In combination with the first aspect, in a possible implementation manner, the guiding piece comprises:

[0010] A sliding sleeve is slidably arranged in the mounting plate, and the lower end of the sliding sleeve has a conical guiding cavity adapted to be embedded in the top of the sample bottle;

[0011] a guide pin hole is arranged in the center of the guide pin;

[0012] an elastic member is sleeved on the sliding sleeve and connected with the mounting plate, and is used for applying a downward elastic thrust to the sliding sleeve.

[0013] In some embodiments, the thrusting member comprises:

[0014] a thrusting frame connected to the bottom surface of the tray;

[0015] a telescopic cylinder vertically connected to the thrusting frame, and an output end of the telescopic cylinder penetrates through the tray upward and is aligned with the sample needle in an up-down direction;

[0016] wherein, when the telescopic cylinder is in a retracted state, the sample bottle located directly below the sample needle is separated from the guide member in an up-down direction, and when the telescopic cylinder is in an extended state, the sample bottle located directly below the sample needle is embedded into the lower end of the guide member upward.

[0017] In an example, the rotary sample feeding assembly comprises:

[0018] a rotary driving member fixedly connected to the bottom surface of the tray, and an output end of the rotary driving member penetrates through the tray upward and is connected with a material stirring wheel;

[0019] a semi-ring baffle arranged on the tray and surrounding the periphery of the material stirring wheel, and a rotary channel is formed between the semi-ring baffle and the material stirring wheel;

[0020] a feeding channel arranged on the tray along the radial direction of the material stirring wheel and in communication with the rotary channel, the feeding channel being used for guiding the sample bottle to enter the rotary channel;

[0021] a discharging channel arranged on the tray along the tangential direction of the material stirring wheel and in communication with the rotary channel, the discharging channel being used for guiding the sample bottle to exit the rotary channel.

[0022] In an example, the peripheral wall of the material stirring wheel is spacedly provided with a plurality of material stirring clamping grooves along the circumferential direction of the material stirring wheel, and the material stirring clamping grooves are adapted to partially embed the sample bottle.

[0023] In a possible implementation, the feeding channel comprises a first feeding baffle and a second feeding baffle arranged in parallel and at intervals, the first feeding baffle is connected with one end of the semi-ring baffle, and the second feeding baffle extends to the edge of the material stirring wheel.

[0024] In some embodiments, the discharging channel comprises a first discharging baffle and a second discharging baffle arranged in parallel and at intervals, the first discharging baffle is connected with one end of the semi-ring baffle, and the second discharging baffle extends to the edge of the material stirring wheel.

[0025] In an example, the tray is provided with a first avoiding groove and a second avoiding groove; wherein the first avoiding groove is located directly below the feeding channel and is used for embedding the feeding conveyor belt, and the second avoiding groove is located directly below the discharging channel and is used for embedding the discharging conveyor belt.

[0026] For example, the tray is spaced apart along its circumference with a plurality of adjustable support rods, and the mounting plate is connected to each adjustable support rod.

[0027] The micro-sample feeding mechanism has the advantages that, compared with the prior art, the micro-sample feeding mechanism utilizes the mounting plate at the top of the mounting frame as an installation carrier of the material moving arm to improve the relative position accuracy of the sample adding needle and the rotary sample feeding assembly installed on the material moving arm, the rotary sample feeding assembly utilizes the rotating material pushing wheel to make the sample bottles pass through the front of the sample adding needle in an orderly manner, each sample bottle stays in front of the sample adding needle once, and the pusher pushes the sample bottle upward to embed the top of the sample bottle into the guide piece to guide the bottle body, and the guide needle hole guides the needle head of the sample adding needle to penetrate the sealing plug of the sample bottle opening to enter the sample bottle, the movement of the needle head is restricted by the guide needle hole to avoid the sample adding needle from being bent and deformed or broken, the sample adding needle is stably protected, the sample feeding is completed when the sample bottle is in a sealed state, the sample is prevented from being polluted by direct contact with the air, and the sample detection accuracy is improved.

[0028] In a second aspect, the utility model embodiment further provides a biochemical detection sample adding device, including above -mentioned micro -sample feeding mechanism.

[0029] The biochemical detection sample adding device has the advantages that, compared with the prior art, the biochemical detection sample adding device utilizes the micro-sample feeding mechanism, the needle head of the sample adding needle is guided by the guide needle hole to penetrate the sealing plug of the sample bottle opening to enter the sample bottle, the movement of the needle head is restricted by the guide needle hole to avoid the sample adding needle from being bent and deformed or broken, the sample adding needle is stably protected, the sample feeding is completed when the sample bottle is in a sealed state, the sample is prevented from being polluted by direct contact with the air, and the sample detection accuracy is improved. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 The utility model provides a micro -sample feeding mechanism's three -dimensional structure schematic diagram for utility model embodiment provides;

[0031] Figure 2 The utility model provides a micro -sample feeding mechanism's three -dimensional structure schematic diagram for utility model embodiment provides;

[0032] Figure 3 The utility model provides a micro -sample feeding mechanism's three -dimensional structure schematic diagram for utility model embodiment provides;

[0033] Figure 4The top view structure schematic diagram of the rotary sample feeding assembly is adopted in the embodiment of the utility model.

[0034] In the figure: 10, mounting frame; 11, tray; 111, first avoiding groove; 112, second avoiding groove; 12, mounting plate; 13, adjustable support rod; 20, material moving arm; 21, sample adding needle; 30, rotary sample feeding assembly; 300, material poking wheel; 301, material poking slot; 31, rotary driving part; 32, half ring baffle; 321, rotary channel; 33, feeding channel; 331, first feeding baffle; 332, second feeding baffle; 34, material returning channel; 341, first material returning baffle; 342, second material returning baffle; 40, sample bottle; 50, pushing part; 51, pushing frame; 52, telescopic cylinder; 60, guiding part; 61, sliding sleeve; 611, conical guiding cavity; 62, guiding core; 621, guiding needle hole; 63, elastic part; 70, feeding conveying belt; 80, discharging conveying belt. DETAILED DESCRIPTION

[0035] In order to make the technical problems, technical schemes and beneficial effects to be solved in the utility model more clearly understood, the utility model will be further described in detail in combination with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and not to limit the utility model.

[0036] It should be noted that when an element is referred to as "provided on" or "connected to" another element, it can be directly on the other element or indirectly on the other element. It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the present application and simplify the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. The terms "first", "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or several features. In the description of the present application, the meaning of "multiple", "several" is two or more, unless otherwise specifically limited.

[0037] Please refer to Figures 1 to 4The micro-feeding mechanism comprises a mounting frame 10, a rotary sample feeding assembly 30, a pushing piece 50 and a guiding piece 60; wherein the mounting frame 10 is provided with a tray 11 and a mounting plate 12 above the tray 11, the mounting plate 12 is used for mounting a material moving arm 20, the material moving arm 20 is used for mounting a sample adding needle 21 and driving the sample adding needle 21 to vertically lift and drop; the rotary sample feeding assembly 30 is arranged on the tray 11 and has a material pushing wheel 300, the material pushing wheel 300 is used for driving a sample bottle 40 placed on the tray 11 to move along the circumference thereof and pass below the sample adding needle 21; the pushing piece 50 is connected to the tray 11 and vertically aligned with the sample adding needle 21, an output end of the pushing piece 50 is used for upwardly pushing the sample bottle 40 below the sample adding needle 21; the guiding piece 60 is vertically connected to the mounting plate 12 and vertically aligned with the sample adding needle 21, a lower end of the guiding piece 60 is adapted to embed the top of the sample bottle 40, and the guiding piece 60 has a needle guiding hole 621 adapted for the needle head of the sample adding needle 21 to pass through; the guiding piece 60 is used for guiding the bottle body of the sample bottle 40 when the pushing piece 50 lifts up the sample bottle 40, and guiding the needle head to insert into the sample bottle 40.

[0038] It should be noted that the material moving arm 20 and the sample adding needle 21 are both existing structures, specifically, the material moving arm 20 can be an X-Y-Z three-axis spatial motion freedom mechanical arm, or a single freedom mechanical arm which only has the function of driving the sample adding needle 21 to vertically lift and drop, considering that the rotary sample feeding assembly 30 which can drive the sample bottle 40 to pass below the sample adding needle 21 in turn is adopted in the embodiment, therefore, in order to simplify the overall structure, the single freedom material moving arm 20 is preferably adopted (the sample bottle 40 for taking liquid and the sample bottle 40 for adding liquid pass below the sample adding needle 21 in turn, the sample adding needle 21 quantitatively sucks the sample from the previous sample bottle 40 and adds the sucked sample into the sample bottle 40 below, thereby completing quantitative transfer of the sample); the rotary sample feeding assembly 30 can be manually placed with the sample bottle 40 at the material pushing position of the material pushing wheel 300, or can be connected with a conveying line to automatically feed the sample bottle 40 onto the tray 11, and after feeding, return to the conveying line after passing below the sample adding needle 21 under the fluctuation of the material pushing wheel 300, thereby meeting the application requirement of automatic sample detection.

[0039] Compared with the prior art, the micro-injection mechanism provided by the embodiment can improve the relative position accuracy of the sample adding needle 21 installed on the material moving arm 20 and the rotary sample feeding assembly 30 by using the mounting plate 12 at the top of the mounting frame 10 as the mounting carrier of the material moving arm 20. The rotary sample feeding assembly 30 can make the sample bottles 40 orderly pass through the right below of the sample adding needle 21 by using the rotating material shifting wheel 300, so that each sample bottle 40 stays once under the sample adding needle 21, and the pusher 50 pushes the sample bottle 40 upward during the stay time, so that the top of the sample bottle 40 is embedded in the guide 60 to guide the bottle body, and the needle hole 621 guides the needle of the sample adding needle 21 to penetrate the sealing plug of the sample bottle 40 and enter the inside of the sample bottle 40, thereby avoiding the bending deformation or breakage of the needle of the sample adding needle 21 by using the movement constraint of the needle hole 621 on the needle, which not only can protect the sample adding needle 21, but also can complete the sample injection when the sample bottle 40 is in a sealed state, thereby avoiding the pollution caused by the direct contact between the sample and the external air, and improving the detection accuracy of the sample.

[0040] In some embodiments, referring to Figure 2 and Figure 3 , the guide 60 includes a sliding sleeve 61, a guide core 62 and an elastic member 63; the sliding sleeve 61 is slidably arranged on the mounting plate 12, and the lower end of the sliding sleeve 61 has a conical guide cavity 611 suitable for embedding the top of the sample bottle 40; the guide core 62 is screwed to the top end of the sliding sleeve 61, and the center of the guide core 62 is provided with a needle hole 621; and the elastic member 63 is sleeved on the sliding sleeve 61 and connected with the mounting plate 12, and is used for applying a downward elastic pushing force to the sliding sleeve 61.

[0041] When the material shifting wheel 300 fluctuates the sample bottle 40 to the right below of the sample adding needle 21, the pusher 50 pushes the sample bottle 40 upward, so that the top of the sample bottle 40 is embedded in the conical guide cavity 611, and the center of the bottle mouth of the sample bottle 40 is aligned with the needle hole 621 under the guidance of the conical cavity wall of the conical guide cavity 611, then the elastic member 63 (which can be a spring or a spring sheet) starts to compress, and the needle of the sample adding needle 21 starts to penetrate the needle hole 621 and pierce into the sample bottle 40 until the sample bottle 40 rises to the limit position, then the material moving arm 20 drives the sample needle to descend so that the needle further pierces into the sample bottle 40 until the target depth is reached, and the needle rises to reset after the sample injection is completed, and the pusher 50 descends, so that the sliding sleeve 61 resets under the elastic force of the elastic member 63, thereby the needle is separated from the sample bottle 40, and the needle is always protected by the needle hole 621 during the whole process of piercing and extracting the sample bottle 40, thereby avoiding the bending deformation or breakage of the needle, and improving the stability of the liquid transfer.

[0042] As a specific embodiment of the pusher 50, please refer to Figure 2 and Figure 3The pushing piece 50 comprises a pushing frame 51 and a telescopic air cylinder 52. The pushing frame 51 is connected to the bottom surface of the tray 11. The telescopic air cylinder 52 is vertically connected to the pushing frame 51, and the output end thereof penetrates the tray 11 upward and is aligned with the sample adding needle 21. When the telescopic air cylinder 52 is in the retracted state, the sample bottle 40 located directly below the sample adding needle 21 is separated from the guide piece 60. When the telescopic air cylinder 52 is in the extended state, the sample bottle 40 located directly below the sample adding needle 21 is embedded into the lower end of the guide piece 60. The lever of the telescopic air cylinder 52 is arranged to penetrate the tray 11 as the output slide. When the telescopic air cylinder 52 is in the retracted state, the top end of the lever is flush with the top surface of the tray 11, thereby ensuring that the sample bottle 40 can smoothly pass below the sample adding needle 21 under the driving of the material pushing wheel 300, and avoiding the tray 11 from being jammed by the sample bottle 40 due to the hole for the lever. When the lever of the telescopic air cylinder 52 is extended upward, the sample bottle 40 stopped above the lever can be lifted upward. The structure is simple and compact.

[0043] As a specific embodiment of the rotary sample feeding assembly 30, please refer to Figures 2 to 4 The rotary sample feeding assembly 30 comprises a rotary driving piece 31, a semi-ring baffle 32, a feeding channel 33 and a discharging channel 34. The rotary driving piece 31 is fixedly connected to the bottom surface of the tray 11, and the output end thereof penetrates the tray 11 upward and is connected with the material pushing wheel 300. The semi-ring baffle 32 is arranged on the tray 11 and surrounds the periphery of the material pushing wheel 300. The semi-ring baffle 32 and the material pushing wheel 300 form a rotary channel 321 therebetween. The feeding channel 33 is arranged on the tray 11 along the radial direction of the material pushing wheel 300 and is communicated with the rotary channel 321. The feeding channel 33 is used for guiding the sample bottle 40 to enter the rotary channel 321. The discharging channel 34 is arranged on the tray 11 along the tangential direction of the material pushing wheel 300 and is communicated with the rotary channel 321. The discharging channel 34 is used for guiding the sample bottle 40 to discharge from the rotary channel 321.

[0044] The rotary driving piece 31 can be a servo motor or a stepping motor, which can accurately control the intermittent rotation angle of the material pushing wheel 300, thereby ensuring that the material pushing wheel 300 can accurately push the sample bottle 40 to the position directly below the sample adding needle 21 one by one, and avoiding the situation that the sample bottle 40 cannot be guided by the guide piece 60 due to the deviation of the stopping position. The semi-ring baffle 32 and the material pushing wheel 300 form the rotary channel 321 which can restrict the rotary motion track of the sample bottle 40. On this basis, the feeding channel 33 and the discharging channel 34 can realize the automatic feeding of the sample bottle 40 to the position below the sample adding needle 21 and the automatic discharging of the sample bottle 40 after the sample adding is completed. Thus, the automatic feeding and discharging can be realized by the connection with the conveying line of the sample bottle 40, and the automation of the sample detection is improved.

[0045] Specifically, as Figure 4As shown, the peripheral wall of the stirring wheel 300 in the embodiment is provided with a plurality of stirring grooves 301 spaced along the circumferential direction, and the stirring grooves 301 are adapted to partially embed the sample bottles 40. The stirring grooves 301 can be semicircular in shape matching the outer diameter of the sample bottles 40, and the sample bottles 40 are embedded in the stirring grooves 301 to form positioning constraints and move in the rotation channel 321 along with the rotation of the stirring wheel 300, which facilitates the feeding and discharging of the sample bottles 40 into the rotation channel 321.

[0046] In some possible implementation manners, referring to Figure 1 and Figure 4 , the feeding channel 33 includes first and second feeding baffles 331 and 332 arranged in parallel and spaced apart, the first feeding baffle 331 is connected to one end of the half-ring baffle 32, and the second feeding baffle 332 extends to the edge of the stirring wheel 300. Since the feeding channel 33 is in communication with the rotation channel 321 along the radial direction of the stirring wheel 300, only an external force needs to be applied to the sample bottles 40 to move them close to the stirring wheel 300, and when the stirring grooves 301 are aligned with the feeding channel 33, the sample bottles 40 can be driven by the stirring wheel 300 to enter the rotation channel 321 from the position where the first feeding baffle 331 and the half-ring baffle 32 are connected, thereby achieving smooth feeding. Specifically, the external force applied to the sample bottles 40 in the feeding channel 33 can be a conveying line driving the sample bottles 40 to move, and of course, if manual feeding is used, the sample bottles 40 in the feeding channel 33 can be directly pushed by hand.

[0047] In some embodiments, the discharging channel 34 adopts a structure as shown in Figure 4 , which includes first and second discharging baffles 341 and 342 arranged in parallel and spaced apart, the first discharging baffle 341 is connected to one end of the half-ring baffle 32, and the second discharging baffle 342 extends to the edge of the stirring wheel 300. The tangentially arranged angle of the discharging channel 34 can enable the sample bottles 40 to be blocked by the second discharging baffle 342 when the sample bottles 40 are driven by the stirring wheel 300 to move to the position aligned with the discharging channel 34, so that the sample bottles 40 are separated from the stirring grooves 301 and enter the discharging channel 34, and are returned to the conveying line along the discharging channel 34 under the blocking and restraining action of the first and second discharging baffles 341 and 342 on the two sides of the sample bottles 40. Of course, if manual feeding and discharging are used, the sample bottles 40 discharged into the discharging channel 34 can be directly taken out.

[0048] It should be noted that, in order to improve the smoothness of feeding and discharging of the rotation channel 321, please refer to Figure 1 and Figure 4The tray 11 is provided with a first avoiding groove 111 and a second avoiding groove 112; wherein the first avoiding groove 111 is located directly below the feeding channel 33 and is used for embedding the feeding conveying belt 70, and the second avoiding groove 112 is located directly below the discharging channel 34 and is used for embedding the discharging conveying belt 80. The feeding conveying belt 70 and the discharging conveying belt 80 are part of an automatic conveying line, and the feeding conveying belt 70 is used to ensure that the sample bottle 40 is always driven in the feeding channel 33, so that the sample bottle 40 can enter the discharging slot 301 and be driven into the rotary channel 321; meanwhile, when the sample bottle 40 is blocked by the second discharging baffle 342 to the discharging channel 34, the sample bottle 40 can fall on the discharging conveying belt 80, so that the sample bottle 40 is conveyed to the target position by the discharging conveying belt 80, thereby realizing the automation of the whole sample adding station.

[0049] It should be noted that, in some embodiments, referring to Figure 1 The tray 11 is provided with a first avoiding groove 111 and a second avoiding groove 112; wherein the first avoiding groove 111 is located directly below the feeding channel 33 and is used for embedding the feeding conveying belt 70, and the second avoiding groove 112 is located directly below the discharging channel 34 and is used for embedding the discharging conveying belt 80. The feeding conveying belt 70 and the discharging conveying belt 80 are part of an automatic conveying line, and the feeding conveying belt 70 is used to ensure that the sample bottle 40 is always driven in the feeding channel 33, so that the sample bottle 40 can enter the discharging slot 301 and be driven into the rotary channel 321; meanwhile, when the sample bottle 40 is blocked by the second discharging baffle 342 to the discharging channel 34, the sample bottle 40 can fall on the discharging conveying belt 80, so that the sample bottle 40 is conveyed to the target position by the discharging conveying belt 80, thereby realizing the automation of the whole sample adding station.

[0050] Based on the same inventive concept, in combination Figures 1 to 4 It should be noted that, in some embodiments, referring to

[0051] The biochemical detection sample adding device provided by the embodiment avoids the bending deformation or fracture of the sample adding needle 21 by using the guide needle hole 621 to guide the needle head of the sample adding needle 21 to penetrate the sealing plug of the sample bottle 40 and enter the inside of the sample bottle 40, thereby avoiding the bending deformation or fracture of the sample adding needle 21 by using the movement constraint of the needle head by the guide needle hole 621, not only achieving the stable protection of the sample adding needle 21, but also completing the sample adding when the sample bottle 40 is in a sealed state, thereby avoiding the pollution caused by the direct contact between the sample and the external air, and being beneficial to improving the sample detection precision.

[0052] The above only describes preferred embodiments of the utility model, and does not limit the utility model, and any modification, equivalent replacement and improvement made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. Micro-injection mechanism, characterized in that: include: A mounting frame, comprising a tray and a mounting plate located above the tray, wherein the mounting plate is used to mount a material transfer arm, and the material transfer arm is used to mount a sample injection needle and drive the sample injection needle to move vertically upward and downward; A rotary sample feeding assembly is provided on the tray and has a feeding wheel, which is used to drive the sample bottle placed on the tray to move along its circumference and pass directly under the sample injection needle; A pushing member connected to the tray and aligned vertically with the sample injection needle, wherein the output end of the pushing member is used to push upward the sample bottle located directly below the sample injection needle; The guide piece is vertically connected to the mounting plate and aligned with the sample injection needle in upper and lower positions. The lower end of the guide piece is suitable for embedding into the top of the sample bottle, and the guide piece has a guide needle hole suitable for the needle tip of the sample injection needle to pass through. The guide piece is used to guide the body of the sample bottle when the pushing piece lifts the sample bottle, and guide the needle tip to insert into the sample bottle.

2. The micro-injection mechanism according to claim 1, characterized in that: The guide member comprises: A sliding sleeve is slidably mounted on the mounting plate, wherein the lower end of the sliding sleeve has a conical guide cavity suitable for the top of the sample bottle to be embedded; A guide core is screwed to the top end of the sliding sleeve, and the center of the guide core is provided with the guide pin hole; The elastic member is sleeved on the sliding sleeve and connected to the mounting plate, and is used for applying a downward elastic pushing force to the sliding sleeve.

3. The micro-injection mechanism according to claim 1, characterized in that: The pushing member comprises: A push frame connected to the bottom surface of the tray; A telescopic cylinder is vertically connected to the push frame, with an output end passing through the tray upward and aligned with the sample injection needle; When the telescopic cylinder is in a retracted state, the sample bottle located directly below the sample needle is separated from the guide piece up and down; when the telescopic cylinder is in an extended state, the sample bottle located directly below the sample needle is upwardly embedded in the lower end of the guide piece.

4. The micro-injection mechanism according to claim 1, characterized in that: The rotary sample delivery component includes: A rotary drive member is fixedly connected to the bottom surface of the tray, and an output end thereof passes upward through the tray and is connected to the paddle wheel; A semi-annular baffle is provided on the tray and surrounds the periphery of the paddle wheel, and a rotation channel is formed between the semi-annular baffle and the paddle wheel; a feeding channel, provided on the tray along the radial direction of the paddle wheel and connected to the rotary channel, the feeding channel being used to guide the sample bottle into the rotary channel; A material return channel is provided on the tray along the tangent direction of the material transfer wheel and is communicated with the rotary channel. The material return channel is used to guide the sample bottle to be discharged from the rotary channel.

5. The micro-injection mechanism according to claim 4, characterized in that: The peripheral wall of the material-dipping wheel is provided with a plurality of material-dipping slots spaced apart along its circumference, and the material-dipping slots are suitable for partially embedding the sample bottle.

6. The micro-injection mechanism according to claim 5, characterized in that: The feed channel includes a first feed baffle and a second feed baffle arranged in parallel and spaced apart. The first feed baffle is connected to one end of the semi-annular baffle, and the second feed baffle extends to the edge of the paddle wheel.

7. The micro-injection mechanism according to claim 5, characterized in that: The material return channel includes a first material return baffle and a second material return baffle that are arranged in parallel and spaced apart. The first material return baffle is connected to one end of the semi-ring baffle, and the second material return baffle extends to the edge of the material feeding wheel.

8. The micro-injection mechanism according to claim 5, characterized in that: The pallet is provided with a first avoidance groove and a second avoidance groove; wherein, the first avoidance groove is located directly below the feed channel and is used to allow the feed conveyor belt to be embedded, and the second avoidance groove is located directly below the return channel and is used to allow the discharge conveyor belt to be embedded.

9. The micro-injection mechanism according to any one of claims 1 to 8, characterized in that: The tray is provided with a plurality of adjustable support rods spaced apart along its circumference, and the mounting plate is connected to each of the adjustable support rods.

10. A biochemical detection sample adding device, characterized in that: The invention comprises the micro-injection mechanism as described in any one of claims 1 to 9.

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