Connector and brain imaging system based on optical fiber bundle

Abstract

The utility model relates to the technical field of optical detection, in particular to a connector and a brain imaging system based on an optical fiber bundle. The connector comprises a fixed part, a movable part and a screw, the fixing piece comprises a connecting channel and a screw hole, a first opening of the connecting channel is located in the upper surface of the fixing piece, a second opening of the connecting channel is located in the lower surface of the fixing piece, the screw hole is located in one side of the connecting channel and extends in the same direction as the connecting channel, and a screw inlet of the screw hole is located in the upper surface of the fixing piece. The movable part comprises an optical fiber bundle channel and a screw channel, an optical fiber bundle inlet of the optical fiber bundle channel is located on the upper surface of the movable part, an optical fiber bundle outlet of the optical fiber bundle channel is located on the lower surface of the movable part, the optical fiber bundle channel is inserted into the connecting channel from the first opening, and the screw channel is located above the screw hole and aligned with the screw hole; a stud of the screw extends into the screw hole from the screw channel, a clamping ring is fixedly arranged on the stud, and the movable part is clamped between the clamping ring and a nut of the screw.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of optical detection technology, in particular to a connector and a brain imaging system based on an optical fiber bundle. BACKGROUND

[0002] Long-term real-time observation and recording of the neuron activity of the relevant brain area of an experimental animal in a specific behavioral paradigm is an important method for analyzing the functional mechanism and encoding characteristics of the mammalian brain. Experimental animals in a free activity state are closer to their natural living state and can complete more behaviors such as mating and social interaction, which is an ideal experimental state desired by neuroscience research. Synchronous observation of neural activity in multiple brain areas that make up a neural circuit can provide more abundant information for analyzing the functional mechanism of the relevant brain area at the circuit level.

[0003] In recent years, fluorescent probes for reflecting the process of neural activity or neurotransmitter release and specific labeling techniques have developed rapidly, and the application of optical imaging technology to neuroscience research has shown significant advantages such as high throughput, specificity, and high spatiotemporal resolution, which are different from traditional electrophysiological techniques.

[0004] Imaging through optical fiber mediation is a relatively effective technical solution for realizing single-cell level optical imaging on experimental animals in a free activity state.

[0005] The technical solution of imaging based on optical fiber mediation uses a flexible image transmission optical fiber bundle to realize the transmission of image information on experimental animals, and the imaging device is completely external, can be combined with various existing imaging methods, and the size and selection of the device are completely unrestricted, so that multiple sites can be recorded more conveniently.

[0006] Specifically, the technical solution of imaging based on optical fiber mediation includes an imaging device, an optical fiber bundle, a self-focusing lens, and a connector. One end of the optical fiber bundle is located at the focal plane of the objective lens of the imaging device, the other end of the optical fiber bundle is located at the image plane of the self-focusing lens, the optical fiber bundle and the self-focusing lens are connected through the connector, and the self-focusing lens is implanted in the brain of a living animal through surgery and directly contacts the observed part.

[0007] In the prior art, the distance between the optical fiber bundle and the self-focusing lens is fixed after the connector connects the optical fiber bundle and the self-focusing lens, so as to ensure that the end face of the optical fiber bundle is located on the image plane of the self-focusing lens. However, in practice, the positional relationship between the self-focusing lens and the target region of the brain tissue changes over time, causing the end face of the optical fiber bundle to deviate from the image plane of the self-focusing lens, resulting in the imaging device being unable to image the same target area. CONTENT OF THE UTILITY MODEL

[0008] The application provides a connector and a brain imaging system based on a fiber bundle to solve the problem that the distance between the end face of the fiber bundle and the image plane of the self-focusing lens cannot be adjusted in the prior art.

[0009] In a first aspect, the application provides a connector for connecting the end face of a fiber bundle to the image plane of a self-focusing lens, the connector comprising:

[0010] a fixed part, the fixed part comprising a connecting channel and a screw hole, a first opening of the connecting channel being located on the upper surface of the fixed part, a second opening of the connecting channel being located on the lower surface of the fixed part, the second opening being used for allowing the self-focusing lens to enter the connecting channel, the screw hole being located on one side of the connecting channel and extending in the same direction as the connecting channel, a screw inlet of the screw hole being located on the upper surface of the fixed part;

[0011] a movable part, the movable part comprising a fiber bundle channel and a screw channel, a fiber bundle inlet of the fiber bundle channel being located on the upper surface of the movable part, a fiber bundle outlet of the fiber bundle channel being located on the lower surface of the movable part, the fiber bundle channel being inserted into the connecting channel from the first opening to allow the movable part to move up and down relative to the fixed part, the screw channel being located above and aligned with the screw hole; and

[0012] a screw, a screw post of the screw extending into the screw hole from the screw channel, a snap ring being fixedly arranged on the screw post, the movable part being clamped between the snap ring and the nut of the screw;

[0013] wherein the screw is rotated in the screw hole to drive the movable part to move up and down relative to the fixed part.

[0014] In a second aspect, the application provides a brain imaging system based on a fiber bundle, comprising:

[0015] an imaging device;

[0016] a self-focusing lens;

[0017] a fiber bundle, an end face of a first end of the fiber bundle being located on the focal plane of the objective lens of the imaging device, an end face of a second end of the fiber bundle being located on the image plane of the self-focusing lens; and

[0018] the connector of the first aspect, the second end of the fiber bundle being fixedly arranged in the fiber bundle channel, the self-focusing lens extending into the connecting channel from the second opening of the connecting channel.

[0019] Compared with the prior art, the application has at least the following beneficial effects:

[0020] The optical fiber channel of the movable part is inserted into the connecting channel of the fixed part, so that the movable part and the fixed part can move relatively, when the screw is rotated in the screw hole, the nut and the snap ring can drive the movable part to move up and down on the fixed part, thereby adjusting the distance between the end face of the lower end of the optical fiber bundle and the image plane of the self-focusing lens, so that the end face of the lower end of the optical fiber bundle is located on the image plane of the self-focusing lens, and the imaging device can clearly image. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 A schematic diagram of a brain imaging system based on an optical fiber bundle of an embodiment of the present application is shown installed on a mouse.

[0022] Figure 2 A front view of a connector of an embodiment of the present application is shown.

[0023] Figure 3 A top view of a connector of an embodiment of the present application is shown.

[0024] Figure 4 A cross-sectional view of Figure 3 at A-A is shown.

[0025] Figure 5 A cross-sectional view of a connector of an embodiment of the present application is shown.

[0026] Figure 6 A schematic diagram of an imaging device of an embodiment of the present application is shown.

[0027] REFERENCE NUMERALS:

[0028] 1, imaging device; 11, first light source; 110, first filter; 12, second light source; 120, second filter; 13, first dichroic mirror; 14, second dichroic mirror; 15, objective lens; 16, third filter; 17, tube lens; 18, camera;

[0029] 2, optical fiber bundle;

[0030] 3, connector; 31, movable part; 311, optical fiber bundle channel; 3111, optical fiber bundle inlet; 3112, optical fiber bundle outlet; 312, screw channel; 3121, clamping groove; 313, body; 314, connecting part; 32, fixed part; 321, connecting channel; 3211, first opening; 3212, second opening; 322, screw hole; 3221, screw inlet; 323, jackscrew hole; 33, screw; 331, stud; 332, nut;

[0031] 4, snap ring; 41, through hole. DETAILED DESCRIPTION

[0032] The technical solutions of the present application will be further described below in conjunction with the accompanying drawings and specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all.

[0033] Some orientation words are defined in the present application. Unless otherwise stated, the orientation words such as "upper", "lower", "left", "right", "inner", "outer" are used for the convenience of understanding, and thus do not constitute a limitation on the scope of protection of the present application.

[0034] In the present application, unless otherwise explicitly specified and limited, "on" or "under" of the first feature to the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "on" of the first feature to the second feature include that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. "Under", "below" and "under" of the first feature to the second feature include that the first feature is directly below and obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0035] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0036] As shown in Figure 1 The embodiment of the present application provides a brain imaging system based on a fiber bundle, which comprises an imaging device 1, a fiber bundle 2, a connector 3 and a self-focusing lens (not shown).

[0037] The self-focusing lens is implanted in the skull of a living animal (such as a mouse) by surgery, one end of the self-focusing lens is in direct contact with the observed part, and the other end of the self-focusing lens extends to the outside of the living animal.

[0038] The connector 3 can be fixedly arranged on the skull of the living animal by dental cement, and the other end of the self-focusing lens extends into the connector 3.

[0039] As shown in Figure 1As shown, the number of optical fiber bundles 2 can be one or more. The lower end of the optical fiber bundle 2 is connected to the corresponding connector 3, and the end face of the lower end of the optical fiber bundle 2 is located on the image plane of the self-focusing lens. The upper end of the optical fiber bundle 2 is connected to the imaging device 1, in particular, to the focal plane of the objective lens 15 of the imaging device 1.

[0040] When the fiber optic bundle-based brain imaging system of the embodiment of the present application is in use, the imaging device 1 emits excitation light, which is transmitted to the self-focusing lens through the fiber optic bundle 2 and finally irradiated on the observed part. The observed part emits fluorescence in response to the excitation light, which is transmitted to the fiber optic bundle 2 through the self-focusing lens and finally received and imaged by the imaging device 1.

[0041] like Figure 2 As shown, an embodiment of the present application provides a connector 3, which can connect the end face of the optical fiber bundle 2 to the image plane of the self-focusing lens.

[0042] like Figures 2 to 5 As shown, connector 3 includes a fixed member 32, a movable member 31, and a screw 33. Fixed member 32 is used to be fixedly mounted on the skull of a living animal and is connected to the self-focusing lens. Movable member 31 is used to connect to optical fiber bundle 2. Movable member 31 is connected to fixed member 32, and screw 33 can adjust the distance between movable member 31 and fixed member 32 so that the end face of optical fiber bundle 2 is located on the image plane of the self-focusing lens.

[0043] like Figure 4 and Figure 5 As shown, the fixing member 32 is generally in the shape of a rectangular parallelepiped. The lower end of the fixing member 32 can be fixed on the skull of a living animal using dental cement. The fixing member 32 includes a connecting channel 321, which is used to allow the movable member 31 to enter the interior of the fixing member 32 on the one hand, and to allow the self-focusing lens to enter the interior of the fixing member 32 on the other hand. Specifically, the connecting channel 321 includes a first opening 3211 and a second opening 3212. The first opening 3211 is located on the upper surface of the fixing member 32, and the first opening 3211 is used to allow the movable member 31 to enter the connecting channel 321. The second opening 3212 is located on the lower surface of the fixing member 32, and the second opening 3212 is used to allow the self-focusing lens to enter the connecting channel 321.

[0044] like Figure 4 and Figure 5As shown, the movable piece 31 comprises a fiber bundle channel 311, which comprises a fiber bundle inlet 3111 and a fiber bundle outlet 3112. The fiber bundle inlet 3111 is located on the upper surface of the movable piece 31, and the fiber bundle outlet 3112 is located on the lower surface of the movable piece 31. The fiber bundle 2 can be inserted into the fiber bundle channel 311 from the fiber bundle inlet 3111 and fixed in the fiber bundle channel 311 by glue. Due to the presence of the fiber bundle outlet 3112, the lower end of the fiber bundle 2 is exposed on the lower surface of the movable piece 31. In addition, the fiber bundle channel 311 can be inserted into the connecting channel 321 from the first opening 3211 to enable the movable piece 31 to move up and down relative to the fixed piece 32, and the lower end of the fiber bundle 2 is in communication with the connecting channel 321, so that the fiber bundle 2 is in communication with the self-focusing lens.

[0045] Thus, by adjusting the distance between the movable piece 31 and the fixed piece 32, the end face of the fiber bundle 2 can be located on the image plane of the self-focusing lens.

[0046] It should be noted that the lower end of the fiber bundle 2 can be flush with the fiber bundle outlet 3112. In this way, on the one hand, the lower end of the fiber bundle 2 can be protected, and on the other hand, the lower end of the fiber bundle 2 can be easily cleaned.

[0047] As shown in Figure 4 and Figure 5 As shown, the fixed piece 31 further comprises a screw hole 322, which is located on one side of the connecting channel 321 and extends in the same direction as the connecting channel 321. The screw inlet 3221 of the screw hole 322 is located on the upper surface of the fixed piece 32.

[0048] As shown in Figure 4 and Figure 5 As shown, the movable piece 31 further comprises a screw channel 312, which is located above the screw hole 322 and aligned with the screw hole 322.

[0049] As shown in Figure 5 As shown, the screw post 331 of the screw 33 extends into the screw hole 322 from the screw channel 312, and the snap ring 4 is fixedly arranged on the screw post 331. The movable piece 31 is clamped between the snap ring 4 and the nut 332 of the screw 33.

[0050] Thus, when the screw 33 is rotated in the screw hole 322, the nut 332 and the snap ring 4 can drive the movable piece 31 to move up and down on the fixed piece 32, thereby adjusting the distance between the end face of the lower end of the fiber bundle 2 and the image plane of the self-focusing lens, so that the end face of the lower end of the fiber bundle 2 is located on the image plane of the self-focusing lens.

[0051] As shown in Figure 5As shown, the snap ring 4 includes a through hole 41, the inner diameter of the through hole 41 is greater than the diameter of the stud 331, in the installation, the lower end of the stud 331 first passes through the screw channel 312, and then the snap ring 4 is sleeved on the outer periphery of the stud 331, when the snap ring 4 and the nut 332 are clamped on the movable part 31, the snap ring 4 is fixed between the stud 331 through the glue. At this time, the screw 33 can drive the movable part 31 to move synchronously.

[0052] As shown in the drawings, Figure 5 As shown, the screw channel 312 includes a clamping groove 3121 opened downward, and the snap ring 4 is located in the clamping groove 3121. Therefore, the snap ring 4 is located inside the movable part 31, which is beneficial to reduce the height of the connector 3 as a whole.

[0053] The model of the screw 33 is M1, M1.1 or M1.2. Too small model affects the connection stability and strength between the movable part 31 and the fixed part 32, in addition, the pitch of too small model is the same as M1, M1.1 or M1.2, which cannot improve the adjustment accuracy. Too large model, the pitch is too large, which is not conducive to ensure the adjustment accuracy.

[0054] In the embodiment of the application, the length or width of the fixed part 32 is not more than 5mm, so as to set multiple connectors on the head of a living animal. Taking a mouse as an example, the available area of the skull of the mouse is about 10x10mm, considering multi-site recording, the connector 3 should be as small as possible, if two connectors 3 need to be set in one direction, the size of the connector 3 in that direction cannot exceed 5mm.

[0055] As an example, in the embodiment of the application, the approximate size of the connector 3 is: 4.5mm (height) x 4.0mm (length) x 2.5mm (width).

[0056] In addition, in the embodiment of the application, the weight of the connector 3 is not more than 1g. Because it is generally considered that the head of a mouse should not exceed 5g, in order to improve the throughput of the experiment, multiple connectors will be worn on the head of a mouse at the same time, the smaller the weight of the connector, the more brain areas can be imaged at the same time. In other words, under the condition of wearing the same number of connectors, the smaller the weight of the connector, the more comfortable the mouse is, and the less the influence on its behavior. As an example, in the embodiment of the application, the weight of the connector 3 can be 0.16g.

[0057] The material of the connector 3 can be aluminum alloy, titanium alloy, PVC, etc. The movable part 31 and the fixed part 32 can be machined or 3D printed.

[0058] As shown in the drawings, Figure 2As shown, the movable member 31 includes a body 313 and a connecting portion 314. The connecting portion 314 extends downward from the lower end of the body 313. The fiber bundle channel 311 is located in the body 313 and the connecting portion 314. The screw channel 312 is located in the body 313. The connecting portion 314 extends from the first opening 3211 into the connecting channel 321.

[0059] like Figure 2 As shown, the fixing member 32 further includes a jackscrew hole 323 provided on a side surface of the fixing member 32, and the jackscrew hole 323 is communicated with the connecting passage 321. The connector 3 further includes a jackscrew (not shown), which is movably provided in the jackscrew hole 323 and can abut against the outer wall of the connecting portion 314 to relatively fix the movable member 31 and the fixing member 32.

[0060] When the distance between the movable member 31 and the fixed member 32 needs to be adjusted, the top screw can be separated from the outer wall of the connecting portion 314, so that the movable member 31 can move up and down relative to the fixed member 32. After the adjustment, the top screw can be re-attached to the outer wall of the connecting portion 314, so that the movable member 31 and the fixed member 32 are relatively fixed.

[0061] like Figure 6 As shown, the imaging device 1 includes a first excitation light component, a second excitation light component, a first dichroic mirror 13, a second dichroic mirror 14 and an imaging component.

[0062] The first excitation light assembly is configured to emit a first excitation light of a first wavelength to excite a first fluorescence in a target area. Specifically, the first excitation light assembly includes a first light source 11 and a first filter 110 .

[0063] The second excitation light assembly is configured to emit second excitation light of a second wavelength to excite second fluorescence in the target area, wherein the second wavelength is different from the first wavelength. Specifically, the second excitation light assembly includes a second light source 12 and a second filter 120 .

[0064] The first dichroic mirror 13 integrates the first excitation light and the second excitation light into a common optical path.

[0065] The second dichroic mirror 14 reflects the first excitation light and the second excitation light in the common optical path to the objective lens 15 , and transmits the first fluorescence and the second fluorescence from the target tissue.

[0066] The imaging component (eg, camera 18 ) is configured to image the first fluorescence and the second fluorescence. In addition, a third filter 16 and a tube lens 17 may be provided between the camera 18 and the second dichroic mirror 14 .

[0067] When the fluorescent probe is excited by light with a wavelength near the isosbestic point, the intensity of the fluorescent signal is irrelevant or weakly related to the increase in the concentration of calcium ions, and thus can be used as a reference signal to eliminate the non-activity-related interference that may exist in the fluorescent signal at other wavelengths, so as to more accurately reflect the change in the neuron activity. Taking the calcium-sensitive fluorescent protein as an example, the isosbestic point of the commonly used calcium-sensitive fluorescent protein is near 405-415 nm, and thus a reference signal channel of 405-415 nm can be added to the imaging system to realize self-control of the experimental animal.

[0068] As an example, the first light source 11 can be an LED with a center wavelength near 470 nm, and the first filter 110 corresponding to the wavelength. The second light source 12 can be an LED with a center wavelength near 405-415 nm, and the second filter 120 corresponding to the wavelength. The first dichroic mirror 13 is used to combine the light emitted by the two light sources into the same light path, and the light is reflected by the second dichroic mirror 14 into the objective lens 15. The objective lens 15 converges the two colors of excitation light to the focal plane and collects the fluorescent signal. The fluorescent signal is focused by the tube lens 17 onto the camera 18 after being transmitted through the second dichroic mirror 14 and the third filter 16.

[0069] In order to distinguish the fluorescent signals excited by the two color light sources, a time division multiplexing method is adopted, and the first light source 11 and the second light source 12 are alternately turned on to distinguish the different fluorescent signals by different time.

[0070] By analyzing the images, the change curve of the fluorescent signal of the observed part under the two color light sources can be obtained, and the curve of the reference signal and the curve of the fluorescent signal are fitted and calculated to eliminate the non-functional related noise generated by the fiber bundle winding, stretching and the motion of the experimental animal.

[0071] In summary, the connector 3 proposed in the application can be precisely adjusted and is convenient to operate, has small volume and light weight, can be simultaneously installed and used in multiple brain regions, and realizes deep brain multi-site imaging.

[0072] In addition, the brain imaging system based on the fiber bundle proposed in the application adds a reference signal channel in the imaging device 1 to realize self-control of the experimental animal, and improves the experimental efficiency and the accuracy of the experimental data.

[0073] Although the application has been described in detail in the foregoing general description, specific implementation and experiments, some modifications or improvements can be made on the basis of the application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the application, all belong to the scope of protection claimed by the application.

Claims

1. A connector for connecting the end face of an optical fiber bundle to the image plane of a self-focusing lens, characterized in that: The connector comprises: A fixing member (32), the fixing member (32) comprising a connecting channel (321) and a screw hole (322), a first opening (3211) of the connecting channel (321) being located on the upper surface of the fixing member (32), a second opening (3212) of the connecting channel (321) being located on the lower surface of the fixing member (32), the second opening (3212) being used to allow the self-focusing lens to enter the connecting channel (321), the screw hole (322) being located on one side of the connecting channel (321), and the screw hole (322) and the connecting channel (321) extending in the same direction, and a screw entrance (3221) of the screw hole (322) being located on the upper surface of the fixing member (32); a movable member (31), the movable member (31) comprising a fiber bundle channel (311) and a screw channel (312), the fiber bundle inlet (3111) of the fiber bundle channel (311) being located on the upper surface of the movable member (31), the fiber bundle outlet (3112) of the fiber bundle channel (311) being located on the lower surface of the movable member (31), the fiber bundle channel (311) being inserted into the connecting channel (321) from the first opening (3211), so that the movable member (31) can move up and down relative to the fixed member (32), the screw channel (312) being located above the screw hole (322) and aligned with the screw hole (322); and A screw (33), wherein a stud (331) of the screw (33) extends from the screw channel (312) into the screw hole (322), a snap ring (4) is fixedly provided on the stud (331), and the movable member (31) is clamped between the snap ring (4) and the nut (332) of the screw (33); The screw (33) rotates in the screw hole (322) to drive the movable part (31) to move up and down relative to the fixed part (32).

2. The connector according to claim 1, wherein: The snap ring (4) comprises a through hole (41), the inner diameter of the through hole (41) is larger than the diameter of the stud (331), the snap ring (4) is sleeved on the outer circumference of the stud (331) through the through hole (41), and the snap ring (4) and the stud (331) are fixed by glue.

3. The connector according to claim 1, wherein: The screw channel (312) comprises a clamping groove (3121) opened downward, and the clamping ring (4) is located in the clamping groove (3121).

4. The connector according to claim 1, wherein: The model of the screw (33) is M1, M1.1 or M1.

2.

5. The connector according to claim 1, wherein: The length or width of the fixing member (32) does not exceed 5 mm; and / or The weight of the connector does not exceed 1g.

6. The connector according to any one of claims 1 to 5, characterized in that: The movable part (31) includes a body (313) and a connecting portion (314), wherein the connecting portion (314) extends downward from the lower end of the body (313), the optical fiber bundle channel (311) is located in the body (313) and the connecting portion (314), the screw channel (312) is located in the body (313), and the connecting portion (314) is inserted into the connecting channel (321) from the first opening (3211).

7. The connector according to claim 6, wherein: The fixing member (32) further includes a top screw hole (323) provided on a side surface of the fixing member (32), wherein the top screw hole (323) is connected to the connecting channel (321), and the connector further includes: A top screw is movably arranged in the top screw hole (323), and the top screw can abut against the outer wall of the connecting portion (314) to relatively fix the movable part (31) and the fixed part (32).

8. A brain imaging system based on an optical fiber bundle, characterized in that: include: Imaging device (1); Self-focusing lens; An optical fiber bundle (2), wherein the end surface of a first end of the optical fiber bundle (2) is located at a focal plane of an objective lens (15) of the imaging device (1), and the end surface of a second end of the optical fiber bundle (2) is located at an image plane of the self-focusing lens; as well as The connector (3) according to any one of claims 1 to 7, wherein the second end of the optical fiber bundle (2) is fixedly arranged in the optical fiber bundle channel (311), and the self-focusing lens extends into the connecting channel (321) from the second opening (3212) of the connecting channel (321).

9. The fiber bundle-based brain imaging system according to claim 8, wherein: The imaging device (1) comprises: a first excitation light component configured to emit first excitation light of a first wavelength to excite first fluorescence in a target area; a second excitation light assembly configured to emit second excitation light of a second wavelength to excite second fluorescence in the target area, wherein the second wavelength is different from the first wavelength; a first dichroic mirror (13), wherein the first dichroic mirror (13) integrates the first excitation light and the second excitation light into a common optical path; a second dichroic mirror (14) that reflects the first excitation light and the second excitation light in the common optical path toward the objective lens (15), and transmits the first fluorescence and the second fluorescence from the target tissue; and An imaging component is configured to image the first fluorescence and the second fluorescence.

10. The fiber bundle-based brain imaging system according to claim 9, wherein: The first excitation light component and the second excitation light component emit first excitation light and second excitation light alternately.

Images