Explant disinfection device for anther tissue culture

Abstract

The utility model discloses an explant disinfection device for anther tissue culture, which comprises an outer cylinder body, a downwards sunken inner cylinder body is arranged in the outer cylinder body, the upper end of the inner cylinder body and the upper end of the outer cylinder body are mounted into a whole through an annular connecting part, and a stirring area is arranged between the lower end part of the inner cylinder body and the lower end part of the outer cylinder body; an area in the inner cylinder body is set as an explant placing area, meshes are also formed in the cylinder wall of the inner cylinder body, and a disinfectant is poured from the inner cylinder body and enters the outer cylinder body from the meshes of the inner cylinder body. According to the device, the inner cylinder body for placing the explant is arranged in the outer cylinder body, the stirring region is arranged in the region between the inner cylinder body and the outer cylinder body, the stirring motor and the stirring fan blades are correspondingly arranged, and the disinfectant flows through the stirring fan blades, so that the disinfection quality and efficiency are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to anther tissue culture technology field, specifically speaking, an anther tissue culture explant disinfection device. BACKGROUND

[0002] In the prior art, the explant of anther tissue culture needs to be disinfected in the technical process of anther tissue culture. The prior art usually adopts the way of disinfectant to disinfect, for example: the technical solution disclosed in the authorized announcement No. CN 214385463U "a plant tissue culture explant disinfection and cleaning bottle" discloses a filter screen, a bottle body and a bottle cap. The filter screen is uniformly provided with second mesh holes near the bottom end of the outer periphery. The filter screen is uniformly provided with first mesh holes above the second mesh holes of the outer periphery. The top end of the filter screen is fixedly connected with an upper frame. The outer periphery of the filter screen is slidably sleeved with the bottle body. The utility model can accurately control the disinfection time of the explant, prevent the time difference caused by the operation of pouring out the waste liquid or clamping the explant, ensure that the same batch of explants is disinfected at the same time and accurately, and prevent the smaller explants from being discharged with the waste liquid. After the explants are disinfected and poured into sterile water, the sealing property of the bottle can ensure that the sterile water and the explants in the bottle are fully shaken, so that the explants can be cleaned and the clean bench is not polluted.

[0003] For example: the disclosure (announcement) No. CN205830696U discloses "a tissue culture explant disinfection bottle". The technical solution comprises a glass bottle, a filter screen and an explant box. The glass bottle is in the shape of a cylinder, the bottom is closed, and the top is provided with a bottle cap which is used in cooperation with the glass bottle. The filter screen is in the shape of a concave, and the whole filter screen can be freely taken out and put into the glass bottle. The shape of the filter screen is matched with the inner diameter shape of the glass bottle. The two sides of the filter screen are provided with connecting rods, and the ends of the connecting rods are provided with hooks for suspending the filter screen in the glass bottle. The explant box is in the structure of a drawer. The utility model has the advantages that multiple explants can be disinfected in the same disinfection bottle at the same time, time is saved, disinfectant is saved, the filter screen design of the bottle mouth facilitates the replacement of the disinfectant, different explants are placed in different explant boxes for disinfection, the boxes have labels to avoid confusion. The explant box has different sizes of 2-4cm for selection and use, which is suitable for explants of various sizes.

[0004] In the above technical solution, a placing area for placing the explants is usually arranged in the disinfection device, such as a filter screen, and then the disinfectant is introduced into the bottle body. The disinfectant usually disinfects the explants on the filter screen. However, the above technical solution can only disinfect by using the disinfectant which does not flow, and the disinfection effect is not ideal. Since the disinfectant does not flow, some parts of the explants cannot be effectively disinfected, and dead angles are generated, which results in poor disinfection effect.

[0005] In addition, the size of the placement area for placing explants in the prior art is fixed and cannot be adjusted or replaced, which is very inconvenient. There are also explant disinfection devices that can generate flow of disinfectant in the prior art, such as the "tissue culture explant disinfection and dehydration device" disclosed in publication (announcement) No. CN204762668U. The screen mesh cylinder body in the technical solution can be rotated by the handle at the upper end, improving the disinfection efficiency and allowing dehydration.

[0006] However, during the above working process, the rotation of the screen mesh cylinder will cause the explants placed inside to also rotate and move. Since the size of the screen mesh cylinder is fixed, if smaller explants are placed in the screen mesh cylinder, the explants will collide with each other at a high speed, causing damage to the explants and making the operation not worth the effort.

[0007] Therefore, in order to solve the above problems, it is necessary to develop a rotating and moving mechanism for a writing machine, which has a reasonable and stable structure, a simple design, and meets the requirements of writing machine trajectory diversity. Content of the utility model

[0008] The utility model aims at the deficiencies in the prior art and provides an explant disinfection device for anther tissue culture.

[0009] An explant disinfection device for anther tissue culture includes an outer cylinder body, a downwardly recessed inner cylinder body is arranged inside the outer cylinder body, the upper end of the inner cylinder body is integrally installed with the upper end of the outer cylinder body through an annular connecting part, a stirring area is arranged between the lower end of the inner cylinder body and the lower end of the outer cylinder body, the area in the inner cylinder body is arranged as a placement area for explants, mesh holes are further arranged on the cylinder wall of the inner cylinder body, disinfectant is poured into the inner cylinder body, and the disinfectant enters the outer cylinder body from the mesh holes of the inner cylinder body.

[0010] A detachable detachable cylinder body is further arranged in the inner cylinder body, an annular mounting plate extending outward is arranged at the upper end of the detachable cylinder body, a positioning groove corresponding to the edge position of the plate body of the mounting plate is arranged at the upper end of the inner cylinder body, the positioning groove is an annular groove structure, when the detachable cylinder body is placed in the inner cylinder body, the edge position of the plate body of the mounting plate is correspondingly placed in the positioning groove, mesh holes are correspondingly arranged on the detachable cylinder body, and a flow gap is left between the lower end of the detachable cylinder body and the lower end of the inner cylinder body.

[0011] An annular mounting plate extending outward is arranged at the upper end of the detachable cylinder body, a positioning groove corresponding to the edge position of the plate body of the mounting plate is arranged at the upper end of the inner cylinder body, the positioning groove is an annular groove structure, when the detachable cylinder body is placed in the inner cylinder body, the edge position of the plate body of the mounting plate is correspondingly placed in the positioning groove, mesh holes are correspondingly arranged on the detachable cylinder body, and a flow gap is left between the lower end of the detachable cylinder body and the lower end of the inner cylinder body.

[0012] Further, a stirring motor is correspondingly mounted at the bottom of the outer cylinder body, the main shaft of the stirring motor extends upward into the stirring area, and stirring blades are correspondingly mounted at the shaft end, and the stirring blades are located in the stirring area.

[0013] Further, the lower end of the outer cylinder is also provided with a mounting chamber, and the stirring motor is arranged in the mounting chamber.

[0014] Further, the cylinder cover and the outer cylinder are detachably mounted by threads.

[0015] Further, the mesh holes on the dismounting cylinder and the mesh holes on the inner cylinder have the same aperture, and a flow gap is left between the cylinder wall of the dismounting cylinder and the cylinder wall of the inner cylinder.

[0016] Further, the dismounting cylinder is provided with a plurality of dismounting cylinders, and the inner diameters of the dismounting cylinders are different, so that different sizes of explants can be placed by replacing the dismounting cylinders.

[0017] Further, the inner cylinder is the largest cylinder for storing explants, and after the dismounting cylinder is replaced, the flow gap between the dismounting cylinder and the inner cylinder changes accordingly, and the dismounting cylinder does not tightly contact the cylinder wall of the inner cylinder.

[0018] Beneficial effects: the utility model has the following beneficial effects:

[0019] 1) In the device, the inner cylinder for placing the explants is arranged in the inner part of the outer cylinder, and the stirring area is arranged between the inner cylinder and the outer cylinder, and the stirring motor and the stirring fan blade are arranged correspondingly, the sterilization quality and efficiency are increased by the flow of the sterilization liquid generated by the stirring fan blade;

[0020] 2) The dismounting cylinder which can be replaced is arranged in the inner cylinder, and the dismounting cylinder is provided with a plurality of cylinder structures with different sizes, so that the explants with different sizes can be accommodated, and the structure design is reasonable and convenient to use;

[0021] 3) The dismounting cylinder which can be replaced can also be used with the stirring motor, and since the inner cylinder can be selected according to the size of the explants, the explants placed in the inner cylinder will not collide even when the sterilization liquid flows strongly, so that the integrity of the explants is ensured. DRAWINGS

[0022] Figure 1 It is a structural drawing of the utility model;

[0023] Figure 2 It is Figure 1 an exploded view;

[0024] Figure 3 It is Figure 2 an A-A sectional view;

[0025] Figure 4The utility model discloses a structure schematic diagram of inner cylinder and outer cylinder.

[0026] Figure 5 For Figure 4 The middle B-B section view.

[0027] Wherein, outer cylinder 1;Inner cylinder 2;Connecting portion 3;Stirring area 4;Place area 5;Mesh 6;Dismounting cylinder 7;Mounting plate 8;Positioning slot 9;Overflow gap 10;Cylinder cover 11;Stirring motor 12;Stirring fan blade 13;Explant 101;Mounting chamber 103. Specific embodiments

[0028] The utility model discloses further illustrate in combination with the drawings and specific embodiments, and the embodiment is implemented under the premise of the utility model technical scheme, and it should be understood that these embodiments are only used for illustrating the utility model and are not used for limiting the scope of the utility model.

[0029] As Figure 1 Shown, an anther tissue disinfection device for tissue culture, including outer cylinder 1, the inside of the outer cylinder 1 is provided with the inner cylinder 2 that is concave downward, the upper end of the inner cylinder 2 is installed into an organic whole with the upper end of outer cylinder 1 through annular connecting portion 3, and the lower end of inner cylinder 2 is provided with stirring area 4 between the lower end of outer cylinder 1, the area in the inner cylinder 2 is arranged as the place area 5 of explant 101, and the cylinder wall of inner cylinder 2 is also provided with mesh 6, pour disinfectant from the inner cylinder 2, and the disinfectant enters outer cylinder 1 from the mesh 6 of inner cylinder 2;

[0030] As Figure 2 And Figure 3 Shown, the inner cylinder 2 is also provided with a detachable dismounting cylinder 7, the upper end of the dismounting cylinder 7 is provided with the annular mounting plate 8 that extends to the outside, and the upper end of the inner cylinder 2 is provided with the positioning slot 9 corresponding to the plate body edge position of mounting plate 8, and the positioning slot 9 is annular groove structure, when the dismounting cylinder is placed in the inner cylinder 2, the plate body edge position of mounting plate 8 is placed in the positioning slot 9 correspondingly, the dismounting cylinder 7 is provided with mesh 6 correspondingly, and the overflow gap 10 is left between the lower end of the dismounting cylinder 7 and the lower end of the inner cylinder 2.

[0031] As Figure 4 And Figure 5 Shown, the upper end of outer cylinder 1 is also provided with detachable cylinder cover 11, and the inner cylinder 2 and the dismounting cylinder 7 are fixed in outer cylinder 1 correspondingly by the cylinder cover 11.

[0032] The bottom of outer cylinder 1 is correspondingly provided with stirring motor 12, the main shaft of the stirring motor 12 is inserted into stirring area 4 upwards, and the shaft end is correspondingly provided with stirring fan blade 13, and the stirring fan blade 13 is located in stirring area 4.

[0033] The lower end of the outer cylinder 1 is further provided with a mounting chamber 103, and the stirring motor 12 is correspondingly arranged in the mounting chamber 103.

[0034] The cylinder cover 11 is detachably mounted with the outer cylinder 1 by threads; the mesh hole 6 on the disassembly cylinder 7 and the mesh hole 6 on the inner cylinder 2 have the same aperture, and a flow gap 10 is left between the cylinder wall of the disassembly cylinder 7 and the cylinder wall of the inner cylinder 2.

[0035] A plurality of disassembly cylinders 7 are arranged, and the inner diameters of the disassembly cylinders 7 are different, so that different sizes of explants 101 can be placed by replacing the disassembly cylinders 7.

[0036] The inner cylinder 2 is the largest cylinder for storing the explants 101, and after the disassembly cylinder 7 is replaced, the flow gap 10 between the disassembly cylinder 7 and the inner cylinder 2 changes accordingly, and the disassembly cylinder 7 is not tightly attached to the cylinder wall of the inner cylinder 2.

[0037] The overall working process of the device is as follows: first, the technical scheme of the device needs to select disassembly cylinders with different inner diameters according to the size of the explants to be sterilized, pour the sterilizing solution into the inner cylinder, enter the outer cylinder from the mesh hole, then place the selected disassembly cylinder with the appropriate size in the inner cylinder, then place the explants to be sterilized in the disassembly cylinder, and then cover the outermost cylinder cover, and then start the stirring motor to drive the stirring fan to stir the sterilizing solution.

[0038] The sterilizing solution first drives the sterilizing solution in the outer cylinder to stir, and since the cylinder walls of the inner cylinder and the disassembly cylinder are both provided with mesh holes, the sterilizing solution in the disassembly cylinder can be stirred, on the one hand, the flow speed of the sterilizing solution in the disassembly cylinder should not be too large to avoid damaging the explants; on the other hand, the size of the selected disassembly cylinder in the device is matched with the size of the explants, and the explants in the disassembly cylinder are basically stationary, so the internal explants can be effectively protected.

[0039] By selecting disassembly cylinders with different sizes to adapt to explants of different sizes, and the inner cylinder as the largest storage space for the explants, larger explants can be directly placed in the inner cylinder for sterilization, which is more convenient.

[0040] The above specific embodiment is only a preferred embodiment of the present application, and is not intended to limit the implementation and scope of claims of the present application, and any equivalent changes and modifications made according to the scope of the patent protection of the present application should be included in the scope of the patent application of the present application.

Claims

1. An explant disinfecting device for anther tissue culture, characterized by: The application relates to a sterilization device for explants, which comprises an outer cylinder (1), the inside of the outer cylinder (1) is provided with a downwardly recessed inner cylinder (2), the upper end of the inner cylinder (2) is integrally installed with the upper end of the outer cylinder (1) through an annular connecting part (3), a stirring area (4) is arranged between the lower end of the inner cylinder (2) and the lower end of the outer cylinder (1), the area in the inner cylinder (2) is arranged as a placing area (5) of the explants (101), and mesh holes (6) are further arranged on the cylinder wall of the inner cylinder (2); sterilization liquid is poured into the inner cylinder (2), and the sterilization liquid enters the outer cylinder (1) from the mesh holes (6) of the inner cylinder (2). The inner cylinder (2) is further provided with a detachable detachable cylinder (7), the upper end of the detachable cylinder (7) is provided with an annular mounting plate (8) extending to the outside, the upper end of the inner cylinder (2) is provided with a positioning groove (9) corresponding to the edge position of the mounting plate (8), the positioning groove (9) is an annular groove structure, when the detachable cylinder (7) is placed in the inner cylinder (2), the edge position of the mounting plate (8) is correspondingly placed in the positioning groove (9), the detachable cylinder (7) is correspondingly provided with mesh holes (6), and a flow gap (10) is left between the lower end of the detachable cylinder (7) and the lower end of the inner cylinder (2). The upper end of the outer cylinder (1) is further provided with a detachable cylinder cover (11), the inner cylinder (2) and the detachable cylinder (7) are correspondingly pressed and fixed in the outer cylinder (1).

2. An explant disinfecting device for anther tissue culture according to claim 1, characterized in that: The bottom of the outer cylinder (1) is correspondingly provided with a stirring motor (12), the main shaft of the stirring motor (12) extends into the stirring area (4), and a stirring blade (13) is correspondingly arranged on the shaft end, and the stirring blade (13) is located in the stirring area (4).

3. The explant disinfecting device for anther tissue culture according to claim 2, characterized in that: The lower end of the outer cylinder (1) is further provided with a mounting chamber (103), and the stirring motor (12) is correspondingly arranged in the mounting chamber (103).

4. The explant disinfecting device for anther tissue culture according to claim 1, wherein: The cylinder cover (11) and the outer cylinder (1) are detachably installed in a threaded mode.

5. An explant disinfecting device for anther tissue culture according to claim 1, characterized in that: The mesh holes (6) on the detachable cylinder (7) and the mesh holes (6) on the inner cylinder (2) have the same aperture, and a flow gap (10) is left between the cylinder wall of the detachable cylinder (7) and the cylinder wall of the inner cylinder (2).

6. The explant disinfecting device for anther tissue culture according to claim 5, wherein: The detachable cylinder (7) is provided with a plurality of detachable cylinders (7), the inner diameters of the detachable cylinders (7) are different, and different detachable cylinders (7) are used for placing explants (101) of different sizes.

7. The explant disinfecting device for anther tissue culture according to claim 6, characterized in that: The inner cylinder (2) is used as the largest cylinder for storing explants (101), the flow gap (10) between the detachable cylinder (7) and the inner cylinder (2) changes correspondingly after the detachable cylinder (7) is replaced, and the detachable cylinder (7) is not tightly attached to the cylinder wall of the inner cylinder (2).

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