Stirring and filtering device for urokinase purification

Abstract

The utility model discloses a stirring and filtering device for urokinase purification, which comprises a recycling barrel, a centrifugal filtering device is rotatably mounted in the recycling barrel, and an activated carbon adsorption structure is fixedly mounted at the outlet end of the lower part of the recycling barrel. The centrifugal filtering device is installed, centrifugal force generated through high-speed rotation has a certain stirring effect, impurity particles in a mixture are separated through the filter screen on the outer side of the centrifugal barrel, and compared with an existing mode that the mixture is directly filtered through the filter screen, the filtering speed is higher, and the filtering efficiency is improved; in addition, a sleeve is clamped with a limiting groove in a rotating rod through a limiting rod, the centrifugal barrel can be conveniently pulled out subsequently to clean impurity particles, disassembly and assembly are convenient, a fixing rod arranged on the outer side of the sleeve can rotate along with the centrifugal barrel, and therefore the centrifugal barrel can be conveniently disassembled and assembled. The mixing effect is improved through collision with the mixture in the early stage.

Description

TECHNICAL FIELD

[0001] The utility model relates to urokinase purification technical field, concretely is a kind of urokinase purification is stirred and filters device. BACKGROUND

[0002] Urokinase is mainly used for thrombolytic therapy of thromboembolic diseases, and purification refers to the process of separating specific components from a mixture to obtain a single component. In the process of urokinase purification, filtration is an important step, mainly used to remove insoluble impurities in the mixture. Through filtration, particles and macromolecules in the crude extraction sample can be effectively removed, thereby obtaining a more pure protein sample to provide a basis for subsequent experiments and analysis.

[0003] The prior art discloses a filter device for purification process, which comprises a base, a stirring mechanism fixedly arranged at the top end of the base, a purification cavity, a protective cover, a mounting groove, a motor module, a rotating shaft, a stirring blade, a feeding groove and a liquid passage.

[0004] The above technical solution needs to be stirred first and then filtered, which takes a long time, resulting in low filtering efficiency and affecting the urokinase purification process. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a kind of urokinase purification is stirred and filters device to solve the problems raised in the above background.

[0006] To achieve the above object, the utility model provides the following technical scheme: a kind of urokinase purification is stirred and filters device, including recovery cylinder, centrifugal filter device is rotatably installed in the recovery cylinder, and active carbon adsorption structure is fixedly installed in the outlet end below the recovery cylinder.

[0007] The centrifugal filter device includes a centrifugal cylinder, a sleeve is fixedly connected to the bottom side of the inner part of the centrifugal cylinder, a plurality of fixed rods are arranged on the outer surface of the sleeve in the circumferential direction, the fixed rods are arranged in multiple groups, a plurality of limit rods are arranged on the inner wall of the sleeve in the circumferential direction, and a plurality of arc-shaped sliding blocks are arranged on the circumferential direction of the bottom side near the edge of the centrifugal cylinder.

[0008] In the above technical solution, the centrifugal force generated by high-speed rotation has a certain stirring effect, and the impurity particles in the mixture are separated through the filter screen on the outer side of the centrifugal cylinder. Compared with the existing direct filtration through filter screen, it is faster and improves the filtering efficiency.

[0009] As a further preferred of the technical solution, the bottom side of the inside of the recovery cylinder is fixedly connected with an annular guide plate, the outlet end below the recovery cylinder is fixedly connected with a connecting pipe, the connecting pipe is flush with the low end of the annular guide plate, the top side of the recovery cylinder is clamped with a cover plate, and the bottom side of the recovery cylinder is provided with supporting legs.

[0010] In the above technical solution, the mixture filtered in the recovery cylinder is discharged through the annular guide plate.

[0011] As a further preferred of the technical solution, the left and right ends of the top side of the cover plate are provided with handles, and the front end of the top side of the cover plate is provided with a feeding hopper.

[0012] As a further preferred of the technical solution, the inside bottom of the recovery cylinder and inside the annular guide plate are fixedly connected with a base, an annular sliding groove is opened on the base, and the annular sliding groove is slidingly connected with the outer wall of the arc-shaped sliding block.

[0013] In the above technical solution, the stability in the centrifugal process can be improved.

[0014] As a further preferred of the technical solution, the supporting legs are sleeved with a support, a motor is installed in the middle of the support, a rotating rod is arranged on the output end of the motor, the rotating rod passes through the bottom side of the recovery cylinder and the middle of the base through a bearing, and the rotating rod extends above the recovery cylinder.

[0015] As a further preferred of the technical solution, a limiting groove is arranged in the circumferential direction of the outer surface of the rotating rod above, and the inner wall of the limiting groove and the outer wall of the limiting rod are clamped.

[0016] In the above technical solution, the sleeve is clamped with the limiting rod and the limiting groove on the rotating rod, which facilitates the subsequent extraction of the centrifugal cylinder for cleaning of impurity particles, and facilitates disassembly and assembly.

[0017] As a further preferred of the technical solution, the activated carbon adsorption structure comprises a mounting cylinder, the mounting cylinder is fixedly connected with the connecting pipe, the left and right ends of the inner wall of the mounting cylinder are screw-connected with limiting rings, an activated carbon plate is arranged between the two limiting rings, a sealing cover is screw-connected with the opening end of the mounting cylinder, a discharge pipe is fixedly connected with the middle of the sealing cover, and a valve is arranged on the discharge pipe.

[0018] In the above technical solution, the mixture can be further filtered, the filtering effect is improved, the activated carbon plate is arranged between the two limiting rings, and the limiting ring and the sealing cover are screw-fixed, which facilitates disassembly and replacement or cleaning of the activated carbon plate.

[0019] The utility model provides a kind of stirring filter device for urokinase purification, with the following beneficial effects:

[0020] (1) The utility model discloses a centrifugal filter device is installed, through the centrifugal force of high -speed rotation, have certain stirring effect, and realize the separation of the impurity particle in mixture through the filter screen of centrifugal cylinder outside, compared with the prior art through the filter screen filtering more quickly, improve the efficiency of filtration, and the mixture in the recovery cylinder is discharged through the annular guide plate, in addition, the sleeve adopts the stopper and the stop groove on the rotating rod and is connected, and the subsequent centrifugal cylinder is extracted to clean the impurity particle, and the sleeve outside is provided with the fixed rod, can rotate with the centrifugal cylinder, and the mixing effect is improved through the impact between the mixture in the early stage.

[0021] (2) The utility model discloses a centrifugal filter device is installed, through the centrifugal force of high -speed rotation, have certain stirring effect, and realize the separation of the impurity particle in mixture through the filter screen of centrifugal cylinder outside, compared with the prior art through the filter screen filtering more quickly, improve the efficiency of filtration, and the mixture in the recovery cylinder is discharged through the annular guide plate, in addition, the sleeve adopts the stopper and the stop groove on the rotating rod and is connected, and the subsequent centrifugal cylinder is extracted to clean the impurity particle, and the sleeve outside is provided with the fixed rod, can rotate with the centrifugal cylinder, and the mixing effect is improved through the impact between the mixture in the early stage. DRAWINGS

[0022] Figure 1 It is the structure schematic diagram of the utility model whole;

[0023] Figure 2 It is the local section view of the utility model;

[0024] Figure 3 It is the structure schematic diagram of the utility model centrifugal filter device;

[0025] Figure 4 It is the enlarged view of the utility model B diagram;

[0026] Figure 5 It is the enlarged view of the utility model A diagram;

[0027] In the drawing: 1, recovery cylinder;11, annular guide plate;12, connecting pipe;13, cover plate;131, handle;132, feed hopper;14, base;15, annular sliding slot;16, support leg;2, centrifugal filter device;21, centrifugal cylinder;22, arc sliding block;23, sleeve;24, fixed rod;25, stopper;26, support;27, motor;28, rotating rod;29, limit groove;3, activated carbon adsorption structure;31, installation cylinder;32, limit ring;33, activated carbon plate;34, sealing cover;35, discharge pipe. DETAILED DESCRIPTION

[0028] The technical scheme in the embodiments of the utility model will be clearly and completely described in combination with the drawings in the embodiments of the utility model.

[0029] The utility model provides technical scheme: such as Figure 1 And Figure 2As shown, in this embodiment, a kind of stirring filter device for urokinase purification, including recovery cylinder 1, centrifugal filter device 2 is rotatably installed in recovery cylinder 1, active carbon adsorption structure 3 is fixedly installed in the outlet end below recovery cylinder 1, annular guide plate 11 is fixedly connected in the bottom side of recovery cylinder 1, connecting pipe 12 is fixedly connected with the outlet end below recovery cylinder 1, connecting pipe 12 is flush with the low end of annular guide plate 11, cover plate 13 is clamped in the top side of recovery cylinder 1, support leg 16 is arranged in the bottom side of recovery cylinder 1, handle 131 is arranged in the left and right ends of the top side of cover plate 13, feed hopper 132 is arranged in the top side of cover plate 13, bottom 14 is fixedly connected in the bottom side of recovery cylinder 1 and located in the inside of annular guide plate 11, annular slide groove 15 is formed in bottom 14, annular slide groove 15 is slidably connected with the outer wall of arc-shaped sliding block 22, which can improve the stability during centrifugation.

[0030] As shown in Figure 3 and Figure 4 , centrifugal filter device 2 includes centrifugal cylinder 21, sleeve 23 is fixedly connected to the bottom side of the inside of centrifugal cylinder 21, fixed rod 24 is arranged on the outer surface of sleeve 23 in circumferential direction, multiple groups are arranged on fixed rod 24 in up and down directions, limiting rod 25 is arranged on the inner wall of sleeve 23 in circumferential direction, arc-shaped sliding block 22 is arranged on the bottom side of centrifugal cylinder 21 in circumferential direction close to the edge, support 26 is sleeved between support legs 16, motor 27 is installed in the middle of support 26, rotating rod 28 is arranged on the output end of motor 27, rotating rod 28 passes through the bottom side of recovery cylinder 1 and the middle of bottom 14 through bearing, rotating rod 28 extends above recovery cylinder 1, limiting groove 29 is arranged on the outer surface of rotating rod 28 in circumferential direction, limiting groove 29 inner wall and limiting rod 25 outer wall are clamped, by installing centrifugal filter device 2, by centrifugal force generated by high-speed rotation, it has certain stirring effect, and the separation of impurity particles in mixture is realized by filter screen outside centrifugal cylinder 21, compared with the existing direct filtration through filter screen, it is more rapid, improves the efficiency of filtration, and the mixture in recovery cylinder 1 is discharged through annular guide plate 11, in addition, sleeve 23 is clamped with limiting groove 29 on rotating rod 28 by limiting rod 25, which facilitates subsequent extraction of centrifugal cylinder 21 for cleaning impurity particles, convenient disassembly and assembly, and fixed rod 24 arranged on the outer side of sleeve 23 can rotate with centrifugal cylinder 21, and the mixing effect is improved by impact between it and mixture in early stage.

[0031] As shown in Figure 1 and Figure 5As shown, the activated carbon adsorption structure 3 includes a mounting cylinder 31 fixedly connected with the connecting pipe 12, the left and right ends of the inner wall of the mounting cylinder 31 are screw-connected with limiting rings 32, activated carbon plates 33 are arranged between the two limiting rings 32, a sealing cover 34 is screw-connected with the open end of the mounting cylinder 31, a discharge pipe 35 is fixedly connected with the middle part of the sealing cover 34, a valve is arranged on the discharge pipe 35, through the installation of the activated carbon adsorption structure 3, the mixture can be further filtered, the filtering effect is improved, the activated carbon plates 33 are arranged between the two limiting rings 32, and the limiting rings 32 and the sealing cover 34 are screw-fixed, so that the activated carbon plates 33 can be conveniently disassembled, replaced or cleaned.

[0032] The utility model provides a kind of stirring filter device for urokinase purification, specific working principle is as follows: by feed hopper 132 so that mixture enters centrifuge cylinder 21, impurity particles are separated by centrifuge cylinder 21 outside screen, simultaneously, motor 27 is started to drive rotating rod 28 rotation, so that the limit rod 25 of joint is rotated, further make centrifuge cylinder 21 rotate, separation is carried out by centrifugal force generated by high-speed rotation;The mixture separated out passes through annular guide plate 11 and enters connecting pipe 12, and is further filtered by activated carbon plate 33, until opening valve makes mixture discharge;Subsequently, limit rod 25 can be extracted from rotating rod 28 by opening cover plate 13, centrifuge cylinder 21 can be disassembled, and activated carbon plate 33 can be replaced by disassembling sealing cover 34 and limiting ring 32.

[0033] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A stirred filtration apparatus for the purification of urokinase comprising a recovery cylinder (1) characterised in that: The recovery cylinder (1) is internally rotatably installed with a centrifugal filtering device (2), and the lower outlet end of the recovery cylinder (1) is fixedly installed with an activated carbon adsorption structure (3). The centrifugal filtering device (2) comprises a centrifugal cylinder (21), the bottom side of the inside of the centrifugal cylinder (21) is fixedly connected with a sleeve (23), the outer surface of the sleeve (23) is circumferentially arranged with a fixed rod (24), the fixed rod (24) is arranged in multiple groups up and down, the inner wall of the sleeve (23) is circumferentially arranged with a limiting rod (25), and the bottom side of the centrifugal cylinder (21) is circumferentially arranged with an arc-shaped sliding block (22) near the edge.

2. The agitated filtration apparatus for purifying urokinase according to claim 1, wherein: The bottom side of the inside of the recovery cylinder (1) is fixedly connected with an annular guide plate (11), the lower outlet end of the recovery cylinder (1) is fixedly connected with a connecting pipe (12), the connecting pipe (12) is flush with the lower end of the annular guide plate (11), the top side of the recovery cylinder (1) is clamped with a cover plate (13), and the bottom side of the recovery cylinder (1) is provided with a supporting leg (16).

3. A stirred filtration apparatus for purifying urokinase as claimed in claim 2, wherein: The left and right ends of the top side of the cover plate (13) are provided with handles (131), and the front end of the top side of the cover plate (13) is provided with a feeding hopper (132).

4. The agitated filter apparatus for purifying urokinase according to claim 1, wherein: The bottom side of the inside of the recovery cylinder (1) and inside the annular guide plate (11) are fixedly connected with a base (14), the base (14) is provided with an annular sliding groove (15), and the annular sliding groove (15) is slidably connected with the outer wall of the arc-shaped sliding block (22).

5. The agitated filter apparatus for purifying urokinase as claimed in claim 2, wherein: The supporting legs (16) are sleeved with a support (26), the middle part of the support (26) is provided with a motor (27), the output end of the motor (27) is provided with a rotating rod (28), the rotating rod (28) passes through the bottom side of the recovery cylinder (1) and the middle part of the base (14) through a bearing, and the rotating rod (28) extends above the recovery cylinder (1).

6. A stirred filtration apparatus for purifying urokinase as claimed in claim 5, wherein: The outer surface of the rotating rod (28) is circumferentially arranged with a limiting groove (29), and the inner wall of the limiting groove (29) and the outer wall of the limiting rod (25) are clamped.

7. The agitated filtration apparatus for purifying urokinase according to claim 1, wherein: The activated carbon adsorption structure (3) comprises a mounting cylinder (31), the mounting cylinder (31) is fixedly connected with the connecting pipe (12), the left and right ends of the inner wall of the mounting cylinder (31) are both screw-connected with a limiting ring (32), the activated carbon plate (33) is arranged between the two limiting rings (32), the opening end of the mounting cylinder (31) is screw-connected with a sealing cover (34), the middle part of the sealing cover (34) is fixedly connected with a discharge pipe (35), and the discharge pipe (35) is provided with a valve.

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