Device for increasing dissolved oxygen value of fermentation culture medium
By dispersing compressed air through a stirring and oxygenation mechanism, the problem of low oxygen utilization was solved, thereby improving the dissolved oxygen value of the culture medium and fermentation efficiency.
Patent Information
- Application Number
- CN202422774134.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-14
- Publication Date
- 2025-10-21
- Estimated Expiration
- 2034-11-14
AI Technical Summary
Traditional aeration methods result in low oxygen utilization during aerobic microbial fermentation, and the high-speed compressed airflow accelerates the evaporation of the culture medium, affecting fermentation efficiency.
The system employs a stirring mechanism and an oxygenation mechanism. Compressed air is dispersed through a stirring rod and a ring tube to form microbubbles, thereby improving oxygen utilization and reducing culture medium evaporation.
It increases the dissolved oxygen value of the culture medium, reduces the use of compressed air, reduces the evaporation of the culture medium, and improves the fermentation efficiency.
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Figure CN223458315U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of biological fermentation equipment, especially in improving the device of fermentation medium dissolved oxygen value. BACKGROUND
[0002] In the process of aerobic microbial fermentation, one of the key technical indicators is the dissolved oxygen value, so in the process of aerobic microbial fermentation culture, the dissolved oxygen value of the culture medium is increased by oxygen into the fermentation tank.
[0003] The traditional oxygenation mode is to pass a large amount of compressed air from the bottom of the fermentation tank under stirring, and the oxygen in the compressed air is used to make the dissolved oxygen value in the culture medium meet the growth requirements of microorganisms, but the dispersion effect of compressed air is poor. If compressed air is directly ventilated into the culture medium through one oxygen inlet, the oxygen utilization rate is low, and the high-speed compressed air flow will accelerate the evaporation of the culture medium, thereby changing the nutrient concentration of the culture medium and affecting the efficiency of fermentation. In view of this, the present scheme provides a device for improving the dissolved oxygen value of the fermentation medium to solve the above problems. CONTENT OF THE UTILITY MODEL
[0004] The utility model discloses a device for improving the dissolved oxygen value of the fermentation medium to solve the problems raised in the background art.
[0005] To achieve the above object, the utility model provides the following technical scheme: a device for improving the dissolved oxygen value of the fermentation medium, comprising a fermentation tank, and further comprising:
[0006] A stirring mechanism is vertically connected into the fermentation tank from the top end of the fermentation tank, and is used for stirring the culture medium in the fermentation tank.
[0007] An oxygen inlet mechanism comprises an oxygen inlet part and an oxygen outlet part, the oxygen inlet part is connected into the fermentation tank from the side of the top end of the fermentation tank, the oxygen outlet part is arranged at the bottom of the fermentation tank, and the top of the oxygen outlet part is connected with the bottom of the oxygen inlet part.
[0008] Preferably, the bottom end of the fermentation tank is provided with a plurality of supporting legs, and the bottom of the fermentation tank is provided with a discharge pipe.
[0009] Preferably, the stirring mechanism comprises:
[0010] A stirring motor is arranged at the top end of the fermentation tank.
[0011] A stirring rod is fixedly connected with the output end of the stirring motor at one end, and is vertically rotatably connected into the fermentation tank.
[0012] Preferably, the stirring mechanism further comprises:
[0013] stirring paddles, a plurality of the stirring paddles are sleeved on the outer wall of the stirring rod along the vertical direction;
[0014] a support shaft seat arranged at the bottom of the inner wall of the fermentation tank, and the bottom of the stirring rod is rotatably connected to the middle part of the support shaft seat.
[0015] Preferably, the oxygen delivery part comprises an oxygen delivery pipe, the oxygen delivery pipe is connected to the side of the inner wall of the fermentation tank, the top of the oxygen delivery pipe penetrates the top end of the fermentation tank for oxygen inlet, and the oxygen exhaust part is arranged at the bottom of the oxygen delivery pipe.
[0016] Preferably, the oxygen exhaust part comprises an annular pipe, the side of the top of the annular pipe is connected with the bottom of the oxygen delivery pipe, the side of the bottom end of the annular pipe is surrounded by a plurality of support rods, and the bottom end of the plurality of support rods is fixedly connected with the inner wall of the bottom end of the fermentation tank.
[0017] Preferably, a plurality of oxygen exhaust holes are arranged at the top end of the annular pipe, a plurality of discharge holes are arranged at the bottom end of the annular pipe, and the number ratio of the oxygen exhaust holes to the discharge holes is 5:1.
[0018] Preferably, the outer wall of the oxygen delivery pipe is sleeved with a plurality of connecting frames, and the end of the plurality of connecting frames away from the oxygen delivery pipe is fixedly connected with the inner wall of the fermentation tank.
[0019] The technical effects and advantages of the present application are as follows:
[0020] The present application can reduce the large bubbles of the culture medium during the gas delivery by delivering a compressed air to the oxygen exhaust part through the oxygen delivery part and then dispersing the compressed air into several small compressed air, and can improve the utilization rate of the compressed air and the dissolved oxygen value in the culture medium by dispersing the compressed air into small bubbles through the mechanical stirring, so that the compressed air usage of a certain volume of culture medium can be reduced, thereby reducing the evaporation amount of the culture medium. BRIEF DESCRIPTION OF DRAWINGS
[0021] Figure 1 It is a structure schematic view of the whole application.
[0022] Figure 2 It is a structure sectional view of the whole application.
[0023] Figure 3 It is a structure top view of the oxygen delivery mechanism of the application.
[0024] Figure 4 It is a top view schematic view of the oxygen delivery mechanism of the application.
[0025] Figure 5The utility model discloses an oxygen mechanism structure bottom view.
[0026] Figure 6 The utility model discloses an oxygen mechanism structure bottom view.
[0027] In the drawing: 1, fermenter, 101, support leg, 102, discharge pipe, 2, stirring motor, 201, stirring rod, 202, stirring paddle, 3, oxygen delivery pipe, 4, annular pipe, 401, support rod, 402, oxygen outlet, 403, discharge hole, 5, connecting frame, 6, support shaft seat. DETAILED DESCRIPTION
[0028] The technical scheme in the embodiments of the utility model will be apparently and completely described below with the drawings in the embodiments of the utility model, and apparently, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skilled in the art without creative work belong to the range of the utility model protection.
[0029] The utility model provides a device that improves the oxygen dissolving value of fermentation medium as Figures 1-6 The utility model discloses a device that improves the oxygen dissolving value of fermentation medium, including fermenter 1, the bottom of fermenter 1 is provided with a plurality of support legs 101, and the bottom of fermenter 1 is provided with discharge pipe 102.
[0030] It should be noted that the discharge pipe 102 is erected by the support leg 101, which facilitates the discharge of the fermenter 1 through the discharge pipe 102 from the bottom, and when installing the structure inside the fermenter 1, the tank body can be cut open first, and the sealing welding of the fermenter 1 tank body is carried out after the internal structure is completely set.
[0031] Further comprising:
[0032] The stirring mechanism is vertically connected into the fermenter 1 from the top end of the fermenter 1, and the stirring mechanism is used for stirring the culture medium in the fermenter 1.
[0033] Specifically, the stirring mechanism comprises:
[0034] The stirring motor 2 is arranged at the top end of the fermenter 1.
[0035] One end of the stirring rod 201 is fixedly connected with the output end of the stirring motor 2, and the stirring rod 201 is vertically and rotatably connected into the fermenter 1.
[0036] Further, the stirring mechanism further comprises:
[0037] The stirring paddle 202 is arranged on the outer wall of the stirring rod 201 in the vertical direction.
[0038] The support shaft seat 6 is arranged at the bottom of the inner wall of the fermentation tank 1, the bottom of the stirring rod 201 is rotatably connected to the middle part of the support shaft seat 6, the middle part of the support shaft seat 6 is a bearing structure, a plurality of rod bodies are arranged on the side of the bearing structure and inclined downward, the rod bodies are fixed to the bottom of the fermentation tank 1, the bottom of the stirring rod 201 is rotatably connected to the middle part of the bearing structure, so that the stirring rod 201 can be rotatably supported.
[0039] It should be noted that the stirring motor 2 is arranged, so that the stirring motor 2 is driven to rotate, and the rotation of the support shaft seat 6 is matched, so that the stirring rod 201 can rotate, and the stirring paddle 202 can also rotate under the disturbance of the stirring paddle 202. When the compressed air is introduced into the culture medium, the stirring can disperse the compressed air, change the direction of the airflow, prolong the residence time of the gas in the culture medium, and improve the utilization rate.
[0040] The oxygen supply mechanism includes an oxygen supply part and an oxygen exhaust part. The oxygen supply part is connected to the inside of the fermentation tank 1 from the side of the top end of the fermentation tank 1, and the oxygen exhaust part is arranged at the bottom of the fermentation tank 1. The top of the oxygen exhaust part is connected to the bottom of the oxygen supply part.
[0041] Specifically, the oxygen supply part includes an oxygen supply pipe 3, which is connected to the side of the inner wall of the fermentation tank 1, and the top of the oxygen supply pipe 3 penetrates the top end of the fermentation tank 1 for oxygen supply. The oxygen exhaust part is arranged at the bottom of the oxygen supply pipe 3.
[0042] Further, the oxygen exhaust part includes an annular pipe 4, the top of which is connected to the bottom of the oxygen supply pipe 3, and the bottom of the annular pipe 4 is surrounded by a plurality of support rods 401, and the bottom of the plurality of support rods 401 is fixedly connected to the inner wall of the bottom of the fermentation tank 1.
[0043] It should be noted that the support rods 401 are arranged to suspend the annular pipe 4 at the bottom of the fermentation tank 1, so as to avoid the bottom of the annular pipe 4 being attached to the inner wall of the fermentation tank 1, thereby ensuring that the annular pipe 4 can stably function during operation.
[0044] Further, a plurality of oxygen exhaust holes 402 are formed in the top end of the annular pipe 4, and a plurality of discharge holes 403 are formed in the bottom end of the annular pipe 4, and the number ratio of the oxygen exhaust holes 402 to the discharge holes 403 is 5:1.
[0045] The outer wall of the oxygen supply pipe 3 is provided with a plurality of connecting frames 5, one end of each of the plurality of connecting frames 5 away from the oxygen supply pipe 3 is fixedly connected to the inner wall of the fermentation tank 1, and the connecting frames 5 are used to stably erect the oxygen supply pipe 3 in the fermentation tank 1.
[0046] It should be noted that, since the support rod 401 suspends and supports the annular tube 4, the discharge hole 403 can be used to discharge the culture medium accumulated in the annular tube 4, and ensure the smoothness of the inner wall of the annular tube 4. During the opening of the oxygen discharge hole 402, the opening direction of the oxygen discharge hole 402 should be towards the center of the stirring mechanism. After the compressed air is delivered into the annular tube 4 by the oxygen delivery tube 3, the compressed air is sprayed out through the oxygen discharge hole 402 opened at the top of the annular tube 4, and is dispersed in the culture medium by the mechanical stirring of the stirring mechanism.
[0047] Example 1, verification of the number and aperture size of the air distributor:
[0048] Through experimental comparison, it is found that, under the conditions of constant compressed air pressure of 0.4 MPa and tank pressure of 0.04 MPa, the best effect is achieved by using 50 upward holes and 10 downward holes with an aperture of 5 mm. Compared with the same number of holes, too small aperture will result in insufficient oxygen content in the culture medium during biological fermentation, and too large aperture will increase the use amount of compressed air. When the aperture is the same, too many holes will increase the use amount of compressed air, and too few holes will result in too low oxygen content in the culture medium. The specific experimental data are shown in Table 1.
[0049] Table 1
[0050]
[0051]
[0052] Example 2, experimental comparison according to two air input methods. Under the conditions of constant compressed air pressure of 0.4 MPa and constant total valve opening degree of the front-end air, after the compressed air is input by the fermentation tank 1 bottom air inlet method (the oxygen input mechanism is closed), the tank pressure is kept at 0.04 MPa, the dissolved oxygen meter is calibrated to 100% with the culture solution without input of compressed air, and the dissolved oxygen in the culture medium is 107.2% to 115.5% air. After the compressed air is input by the oxygen input mechanism (the bottom air inlet is closed), the tank pressure is kept at 0.04 MPa, the dissolved oxygen meter is calibrated to 100% with the culture solution without input of compressed air, and the dissolved oxygen in the culture medium is 127.2% to 144.7% air. According to the above analysis, the dissolved oxygen in the culture medium can be increased by 20% to 30% when the compressed air is input by the oxygen input mechanism compared with the compressed air input by the bottom air inlet.
[0053] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.
Claims
1. An apparatus for increasing the dissolved oxygen value of a fermentation medium comprising a fermenter (1), characterised in that, Also include: Stirring mechanism, the stirring mechanism is connected in the fermentation tank (1) by the top end vertical insertion of the fermentation tank (1), the stirring mechanism is used for the stirring of culture medium in the fermentation tank (1); Oxygen supply mechanism, the oxygen supply mechanism includes oxygen supply part and oxygen exhaust part, the oxygen supply part is connected in the fermentation tank (1) by the side insertion of the top end of the fermentation tank (1), the oxygen exhaust part is arranged at the bottom of the fermentation tank (1), the top of the oxygen exhaust part is connected with the bottom of the oxygen supply part.
2. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 1, characterized in that, The bottom end of the fermentation tank (1) is provided with a plurality of supporting legs (101), and the bottom of the fermentation tank (1) is provided with a discharge pipe (102).
3. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 1, characterized in that, The stirring mechanism includes: Stirring motor (2), the stirring motor (2) is arranged at the top end of the fermentation tank (1); Stirring rod (201), one end of the stirring rod (201) is fixedly connected with the output end of the stirring motor (2), and the stirring rod (201) is vertically rotatably connected in the fermentation tank (1).
4. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 3, characterized in that, The stirring mechanism further includes: Stirring paddle (202), a plurality of groups of the stirring paddle (202) are arranged on the outer wall of the stirring rod (201) in the vertical direction; Supporting shaft seat (6), the supporting shaft seat (6) is arranged at the bottom of the inner wall of the fermentation tank (1), and the bottom of the stirring rod (201) is rotatably connected to the middle part of the supporting shaft seat (6).
5. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 1, characterized in that, The oxygen supply part includes an oxygen supply pipe (3), which is connected to the side of the inner wall of the fermentation tank (1), and the top of the oxygen supply pipe (3) penetrates the top end of the fermentation tank (1) for oxygen inlet, and the oxygen exhaust part is arranged at the bottom of the oxygen supply pipe (3).
6. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 5, characterized in that, The oxygen exhaust part includes an annular pipe (4), the side of the top of the annular pipe (4) is connected with the bottom of the oxygen supply pipe (3), and the side of the bottom end of the annular pipe (4) is surrounded by a plurality of supporting rods (401), and the bottom end of the plurality of supporting rods (401) is fixedly connected with the inner wall of the bottom end of the fermentation tank (1).
7. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 6, characterized in that, A plurality of oxygen exhaust holes (402) are formed in the top end of the annular pipe (4), a plurality of discharge holes (403) are formed in the bottom end of the annular pipe (4), and the number ratio of the oxygen exhaust holes (402) to the discharge holes (403) is 5:
1.
8. The device for increasing the dissolved oxygen value of a fermentation medium according to claim 5, characterized in that, The outer wall of the oxygen supply pipe (3) is sleeved with a plurality of connecting frames (5), and one end of the plurality of connecting frames (5) away from the oxygen supply pipe (3) is fixedly connected with the inner wall of the fermentation tank (1).