Cell culture bag
By introducing a breathable composite membrane layer, a multi-layer fiber scaffold, and inert gas filling into the passive cell culture bag, the problem of small culture area is solved, and large-scale cell culture and efficient harvesting are achieved.
Patent Information
- Application Number
- CN202422512280.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-16
- Publication Date
- 2025-10-21
- Estimated Expiration
- 2034-10-16
AI Technical Summary
The existing passive cell culture bags have a small culture area, making it difficult to achieve large-scale cell culture and affecting the harvest of cells and secretions.
A cell culture bag is designed, which includes an air-permeable but water-impermeable composite membrane bag body and an edge sealing portion. Multiple layers of staggered fiber scaffold layers and isolation pads are arranged inside the bag, which is filled with inert gas and equipped with first and second pipes to facilitate the addition and extraction of culture medium and cell suspension.
Significantly increase the cell culture surface area without increasing the space volume, enabling large-scale cell culture, increasing the yield of cells and secretions, and reducing the risk of contamination.
Smart Images
Figure CN223458336U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture device technical field, concretely relates to a cell culture bag. BACKGROUND
[0002] Cell culture bag is divided into initiative and passive. Initiative cell culture bag needs to match equipment to carry out gas exchange, and use is more inconvenient. But passive cell culture bag does not need to match equipment, and carries out gas exchange by the water -resisting membrane material of bag body, and use is more convenient. But, passive cell culture bag mainly relies on the membrane material surface of adhering to the inside of bag body to culture adherent cell, so the culture area is small, and it is difficult to realize large -scale culture of cell, and it is not favorable to harvest more cell or secretion etc. UTILITY MODEL CONTENTS
[0003] Therefore, the technical problem to be solved by the utility model is that passive cell culture bag in the prior art mainly relies on the membrane material surface of adhering to the inside of bag body to culture adherent cell, so the culture area is small, and it is difficult to realize large -scale culture of cell, and it is not favorable to harvest more cell or secretion etc., thereby providing a cell culture bag.
[0004] To solve the above technical problem, the technical scheme of the utility model is as follows:
[0005] The utility model provides a cell culture bag, include: the body, the body includes the composite membrane layer bag body and the sealing portion of edge of breathable but not water -permeable, the body has the inlet and the outlet, culture support is set up in the body, the culture support includes a plurality of layers of stacked setting fiber support layer, each layer fiber support layer has a plurality of fiber silk, and a plurality of fiber silk is parallelly arranged, and the fiber silk of adjacent two fiber support layers is staggered arrangement.
[0006] Further, the culture support is a hydrophilic support.
[0007] Further, the body is filled with inert gas.
[0008] Further, the cell culture bag further includes isolation pad, the front and back of culture support are provided with isolation pad, and the isolation pad limits the contact of culture support and the inner wall of composite membrane layer bag body.
[0009] Further, the number of isolation pad arranged on the single surface of culture support ranges from three to five, and the thickness of single isolation pad ranges from 1mm to 3mm.
[0010] Further, the cell culture bag further comprises a first pipe and a second pipe; one end of the first pipe is inserted into the body through the inlet, and a liquid stopper is arranged on the first pipe for adding culture medium and cell suspension; one end of the second pipe is inserted into the body through the outlet, and the second pipe is used for extracting and sampling the culture solution.
[0011] Further, one end of the first pipe arranged in the body is provided with a first strutting member; the first strutting member comprises two spaced rods, and the edge of the culture support at least partially extends into the space between the two rods.
[0012] Further, one end of the second pipe arranged in the body is provided with a second strutting member; the second strutting member comprises two spaced rods, and the edge of the culture support at least partially extends into the space between the two rods.
[0013] Further, a plurality of separation grooves are arranged in the sealing part, and the plurality of separation grooves divide the sealing part into different areas to limit the sealing part to be connected as a whole.
[0014] Further, the width of a single separation groove ranges from 1cm to 3cm.
[0015] The technical scheme of the cell culture bag has the following advantages:
[0016] The cell culture bag provided by the utility model, which comprises a composite film layer bag body and a sealing part at the edge and is breathable but not water-permeable, is a passive cell culture bag and is more convenient to use; in addition, the culture support arranged in the body can culture cells in a three-dimensional space, significantly increases the cell culture surface area without increasing the space volume, can realize large-scale cell culture, and is beneficial to harvesting more cells or secretions. BRIEF DESCRIPTION OF DRAWINGS
[0017] In order to more clearly illustrate the specific embodiments of the utility model or the technical scheme in the prior art, the following will briefly introduce the drawings needed to be used in the specific embodiments or the prior art description, and obviously, the drawings in the following description are some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.
[0018] Figure 1 It is a schematic view of the cell culture bag in the embodiments of the utility model;
[0019] Figure 2 It is a schematic view of the internal structure of the cell culture bag in the embodiments of the utility model.
[0020] MARKS OF THE DRAWINGS:
[0021] 1, body; 2, composite film layer bag body; 3, inlet; 4, outlet; 5, sealing part; 6, culture support; 7, isolation pad; 8, first pipeline; 9, second pipeline; 10, liquid stopper; 11, rod body; 12, separation groove. DETAILED DESCRIPTION
[0022] The technical solutions of the present application will be described clearly and completely below in conjunction with the drawings. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.
[0023] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0024] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication between two elements inside. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.
[0025] In addition, the technical features involved in the different embodiments of the present application described below can be combined with each other as long as there is no conflict.
[0026] As Figure 1 , Figure 2As shown, the embodiment provides a cell culture bag, which comprises a body 1, the body 1 comprises a bag body 2 of a composite film layer which is breathable but not water-permeable and a sealing part 5 at the edge to ensure gas exchange and bacterial barrier, the body 1 has an inlet 3 and an outlet 4; a culture support 6 can be placed in the body 1 in advance, and then the edge of the body 1 is sealed by hot pressing, and the part bonded together by hot pressing is the sealing part 5. The culture support 6 comprises a plurality of fiber support layers stacked, and has a three-dimensional structure as a whole. For example, the culture support 6 can comprise four fiber support layers, each fiber support layer has a plurality of fiber filaments, and the plurality of fiber filaments are arranged in parallel, and the fiber filaments of adjacent two fiber support layers are arranged in a staggered manner, which can facilitate observation. For example, the diameter of the fiber filament can be 400 microns, and the interval between the adjacent two fiber filaments can be 300 microns.
[0027] The cell culture bag provided by the embodiment comprises a bag body 2 of a composite film layer which is breathable but not water-permeable and a sealing part 5 at the edge, which is a passive cell culture bag and is more convenient to use. Moreover, the culture support 6 arranged in the body 1 can culture cells in a three-dimensional space. Under the premise of not increasing the space volume, the cell proliferation amount can be several times or even dozens of times higher than that of two-dimensional plane culture, the cell culture surface area is significantly increased, large-scale cell culture can be realized, and more cells or secretions can be harvested.
[0028] The culture support 6 can be a hydrophilic support. In actual use, the surface of the culture support 6 can be treated according to requirements. For example, the surface of the culture support 6 can be bombarded by using a high-energy electron beam to make it have a micro concave-convex surface, so as to improve the hydrophilic property. For example, the raw material can also be directly modified to meet the hydrophilic property requirement. For another example, a temperature-sensitive surface grafting technology can be used for surface modification, the culture support 6 is switched between hydrophilic and hydrophobic surface properties by controlling temperature, and the technology can be used for fragile cells to achieve non-destructive cell collection. For example, when the culture support 6 is made, 3d printing, injection molding or adhesive bonding technology can be used to process the finished product of the staggered structure of the fiber filaments of the adjacent two fiber support layers, so as to avoid too many processes, reduce the generation of dust and fine particles, and also make the edge of the culture support 6 smooth and not easy to produce fine particles.
[0029] The body 1 is filled with inert gas. For example, in order to protect the hydrophilic function of the culture support 6, a certain amount of inert gas such as nitrogen can be filled into the body 1 to avoid the hydroxyl group of the hydrophilic layer on the surface of the culture support 6 from being oxidized, so as to avoid reducing the hydrophilic property and ensure that the cells can adhere to the surface of the culture support 6. At the same time, the filling of inert gas can also protect the bag body and prevent it from being damaged.
[0030] The cell culture bag further comprises isolation pads 7, and the front and back surfaces of the culture scaffold 6 are provided with the isolation pads 7, for example, the isolation pads 7 can be bonded on the culture scaffold 6, and the isolation pads 7 limit the contact between the culture scaffold 6 and the inner wall of the composite film layer bag body 2. For example, the isolation pad 7 can be a cylindrical structure. In this way, the culture scaffold 6 can be prevented from adhering to the inner wall of the bag body during the culture process, thereby preventing the death of cells.
[0031] The number of isolation pads 7 provided on one surface of the culture scaffold 6 ranges from three to five, for example, four isolation pads 7 can be provided on one surface of the culture scaffold 6, and the four isolation pads 7 can be respectively arranged near the top corner positions of the culture scaffold 6. The thickness of a single isolation pad 7 ranges from 1 mm to 3 mm, for example, the thickness of a single isolation pad 7 can be 2 mm.
[0032] The cell culture bag further comprises a first pipe 8 and a second pipe 9; one end of the first pipe 8 is inserted into the body 1 through the inlet 3, and a liquid stopping clamp 10 is arranged on the first pipe 8 for adding culture medium and cell suspension; one end of the second pipe 9 is inserted into the body 1 through the outlet 4, and the second pipe 9 is used for extracting and sampling the culture solution. For example, the first pipe 8 and the second pipe 9 can be medical biological pipes meeting medical requirements, and other accessories also meet the medical grade material requirements.
[0033] The end of the first pipe 8 located in the body 1 is provided with a first strutting member; the first strutting member comprises two spaced rod bodies 11 extending in the axial direction of the first pipe 8, and the edge of the culture scaffold 6 at least partially extends into the space between the two rod bodies 11. Similarly, the end of the second pipe 9 located in the body 1 can be provided with a second strutting member; the second strutting member comprises two spaced rod bodies 11 extending in the axial direction of the second pipe 9, and the edge of the culture scaffold 6 at least partially extends into the space between the two rod bodies 11. For example, the inlet 3 and the outlet 4 of the body 1 can be on the same side of the body 1, and at this time, the first pipe 8 and the second pipe 9 are also located on the same side of the body 1. In this way, when the liquid in the bag body needs to be extracted for liquid replacement or the cell suspension after culture needs to be introduced out of the bag body for cell harvesting, the culture bag is subjected to negative pressure suction force, and the upper and lower bag bodies are bonded at the connection, which affects the introduction of the liquid. The existence of the first strutting member and the second strutting member can keep the upper and lower bag bodies in a separated state, thereby ensuring the smooth flow of the liquid.
[0034] The sealing part 5 is provided with a plurality of separation grooves 12, which divide the sealing part 5 into different areas to limit the connection of the sealing part 5 into a whole. For example, if the sealing part 5 of the edge of the body 1 is a whole, the bag body is prone to excessive shrinkage, which affects the appearance and performance of the product. The provision of the separation grooves 12 can reduce shrinkage and make the bag body more beautiful under the premise of ensuring sealing. The width of a single separation groove 12 ranges from 1 cm to 3 cm. For example, the width of a single separation groove 12 can be 2 cm.
[0035] In summary, the cell culture bag in the present application has a simple and feasible process scheme, can realize 3D space cell culture, significantly increases the cell culture surface area under the condition of not increasing the space volume, and the cell proliferation amount can be several times or even dozens of times higher than that of two-dimensional plane culture, which can realize large-scale cell culture and harvest more cells or secretions. Moreover, large-scale cell culture can be performed, and only simple operation is required to meet the use requirements without the need for matching equipment. Moreover, the cells are cultured in a closed manner, which reduces the risk of pollution compared with the traditional open or semi-open culture method. Moreover, after cell culture, low-temperature cryopreservation can be performed according to needs. Moreover, the hydrophilic effect of the surface of the 3D culture scaffold 6 can be maintained for a longer period of time.
[0036] Obviously, the above embodiments are only examples for clear illustration, and are not limitations on the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments need not and cannot be exhausted. The obvious changes or variations derived therefrom are still within the protection scope of the present application.
Claims
1. A cell culture bag, characterized in that, The cell culture bag comprises: a body (1) comprising a gas-permeable but water-impermeable composite film layer bag body (2) and a sealed edge (5), the body (1) having an inlet (3) and an outlet (4); a culture support (6) arranged in the body (1), the culture support (6) comprising a plurality of layers of fiber support layers arranged in layers, each layer of the fiber support layers having a plurality of fiber filaments arranged in parallel, and the fiber filaments of adjacent two layers of the fiber support layers being arranged in a staggered manner.
2. The cell culture bag according to claim 1, wherein the culture support (6) is a hydrophilic support.
3. The cell culture bag according to claim 2, wherein the body (1) is filled with an inert gas.
4. The cell culture bag according to claim 1, wherein further comprising a spacer (7), the front and back surfaces of the culture support (6) are provided with the spacer (7), and the spacer (7) limits the contact between the culture support (6) and the inner wall of the composite film layer bag body (2).
5. The cell culture bag according to claim 4, wherein the number of spacers (7) arranged on a single surface of the culture support (6) ranges from three to five; the thickness of a single spacer (7) ranges from 1 mm to 3 mm.
6. The cell culture bag according to claim 1, wherein further comprising a first pipe (8) and a second pipe (9); one end of the first pipe (8) is inserted into the body (1) through the inlet (3), and a liquid stop clamp (10) is arranged on the first pipe (8) for adding culture medium and cell suspension; one end of the second pipe (9) is inserted into the body (1) through the outlet (4), and the second pipe (9) is used for extracting and sampling the culture solution.
7. The cell culture bag according to claim 6, wherein the end of the first pipe (8) located in the body (1) is provided with a first opening member; the first opening member comprises two spaced rods (11), and the edge of the culture support (6) at least partially extends into the space between the two rods (11).
8. The cell culture bag according to claim 6, wherein the end of the second pipe (9) located in the body (1) is provided with a second opening member; the second opening member comprises two spaced rods (11), and the edge of the culture support (6) at least partially extends into the space between the two rods (11).
9. The cell culture bag according to claim 1, wherein a plurality of separation grooves (12) are arranged in the sealed edge (5), and the separation grooves divide the sealed edge (5) into different areas to limit the connection of the sealed edge (5) into a whole.
10. The cell culture bag according to claim 9, wherein the width of a single separation groove (12) ranges from 1 cm to 3 cm.