Confocal microscopic system based on spatial light modulator
By combining an LED light source and a spatial light modulator, and employing a beam splitter and a projection unit, the problems of slow acquisition speed, low efficiency, and light source diffraction effect in existing confocal microscopy imaging systems are solved, achieving fast and efficient confocal microscopy imaging, supporting multi-wavelength synchronous imaging and flexible encoding.
Patent Information
- Application Number
- CN202423013467.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-06
- Publication Date
- 2025-10-24
- Estimated Expiration
- 2034-12-06
AI Technical Summary
Existing confocal microscopy imaging systems suffer from problems such as slow acquisition speed, low efficiency, inability to overcome light source diffraction effects, inability to achieve flexible confocal effects with fixed encoding methods, limited imaging without fluorescence excitation, and interference from multi-layer illumination information due to depth-of-field limitations.
Using LED light sources and spatial light modulators, multiple regional array point light source projection illuminations are achieved through beam splitting and projection units. Combined with micro-aperture array coding, confocal microscopy imaging is realized using imaging units.
It achieves rapid acquisition, efficient imaging, removal of micropore diffraction effects, flexible encoding, and imaging capabilities without fluorescence excitation, and supports multi-wavelength synchronous confocal imaging.
Smart Images
Figure CN223471186U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to microscopic imaging technical field, concretely relates to a kind of confocal microscopic system based on spatial light modulator. BACKGROUND
[0002] In the process of realizing the utility model, the inventor finds that the existing confocal microscopic imaging scheme at least has the following problems: 1, in the existing confocal microscopic imaging system, the method of galvanometer scanning and micropore array is adopted, the collection speed is slow, and the efficiency is low;2, in the existing confocal microscopic imaging system, laser light source is adopted, and the influence caused by the diffraction effect of light source cannot be overcome;3, in the existing confocal microscopic imaging system, fixed coding mode is adopted, and better confocal effect cannot be realized through flexible coding mode.4, in the existing structured light microscopic imaging system, filter and dichroic mirror are adopted, and imaging cannot be realized under the condition of no fluorescence excitation;5, in the existing epi-illumination microscopic imaging system, the depth of field of image acquisition is limited by imaging system, so that the information of multi-layer illumination is interfered with each other. UTILITY MODEL CONTENT
[0003] The utility model aims at solving the above-mentioned technical problems at least to some extent. To this end, the utility model aims at providing a kind of confocal microscopic system based on spatial light modulator.
[0004] The technical scheme adopted by the utility model is as follows:
[0005] A kind of confocal microscopic system based on spatial light modulator, including light source, light splitting unit, spatial light modulator, projection unit, objective lens, imaging unit and camera, light source, light splitting unit, spatial light modulator are sequentially arranged on the same straight line, objective lens, projection unit, light splitting unit, imaging unit and camera are sequentially arranged on the same straight line;Light splitting unit is used to separate incident light and reflected light, and the light emitted by light source is irradiated to spatial light modulator through light splitting unit;Projection unit projects the illumination light field modulated by spatial light modulator to the surface of target object and forms image;The reflected light of target object reversely passes through the micropore array on spatial light modulator, and forms confocal image;Imaging unit images confocal image to camera.
[0006] Preferably, the light source is LED light source, and a beam shaping unit is arranged between the LED light source and the light splitting unit. The light splitting unit is dichroic mirror or polarization light splitting prism.
[0007] The utility model has the beneficial effects that:
[0008] The utility model provides a kind of confocal microscopic system based on spatial light modulator, array point light source is generated to target by combining LED light source and spatial light modulator projection illumination, realize the full-field scanning imaging to target by multiple regional array point light source projection illumination, realize confocal microscopic imaging;With the advantages of high speed, high efficiency;Can realize to multiple wavelength synchronous confocal imaging;Can remove the diffraction effect of micropore;Can realize flexible micropore array coding;Can be used without fluorescent excitation agent. BRIEF DESCRIPTION OF DRAWINGS
[0009] Figure 1 It is the schematic diagram of the utility model confocal microscopic system based on spatial light modulator.
[0010] In the drawing: 1-light source;2-beam shaping unit;3-splitting unit;4-spatial light modulator;5-projection unit;6-objective lens;7-sample;8-imaging unit;9-camera. DETAILED DESCRIPTION
[0011] The embodiments of the utility model are described in detail below, and the examples of the embodiments are shown in the drawings, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are intended to explain the utility model, and cannot be understood as a limitation of the utility model. The components of the embodiments of the utility model described and shown in the drawings herein can be arranged and designed in various different configurations.
[0012] In the utility model, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected;It can be mechanically connected, or it can be electrically connected;It can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication inside two elements.
[0013] The utility model will be further described below in combination with the drawings and specific embodiments.
[0014] As Figure 1 Shown, a kind of confocal microscopic system based on spatial light modulator of the utility model, including light source 1, beam shaping unit 2, splitting unit 3, spatial light modulator 4, projection unit 5, objective lens 6, imaging unit 8 and camera 9, light source 1, beam shaping unit 2, splitting unit 3, spatial light modulator 4 are sequentially arranged on the same straight line (first straight line), objective lens 6, projection unit 5, splitting unit 3, imaging unit 8 and camera 9 are sequentially arranged on the same straight line (second straight line);First straight line and second straight line are perpendicular.
[0015] The light source 1 is an LED light source, specifically, it can be a white LED or an RBG complex color LED, the spatial light modulator 4 is a DMD spatial light modulator, an LCOS spatial light modulator or a liquid crystal spatial light modulator.
[0016] The light source 1 is an LED light source, specifically, it can be a white LED or an RBG complex color LED, the spatial light modulator 4 is a DMD spatial light modulator, an LCOS spatial light modulator or a liquid crystal spatial light modulator. The light splitting unit 3 is a two-phase color mirror or a polarization light splitting prism.
[0017] The light source 1 is an LED light source, specifically, it can be a white LED or an RBG complex color LED, the spatial light modulator 4 is a DMD spatial light modulator, an LCOS spatial light modulator or a liquid crystal spatial light modulator. The light splitting unit 3 is a two-phase color mirror or a polarization light splitting prism.
[0018] The utility model is not limited to the above optional implementation, anyone can draw other various forms of product under the enlightenment of the utility model, but no matter make any change in its shape or structure, any technical scheme falling into the scope defined by the utility model claims falls within the protection scope of the utility model.
Claims
1. A spatial light modulator based confocal microscopy system, characterized by: It comprises a light source (1), a light splitting unit (3), a spatial light modulator (4), a projection unit (5), an objective lens (6), an imaging unit (8) and a camera (9), the light source (1), the light splitting unit (3) and the spatial light modulator (4) are sequentially arranged on the same straight line, the objective lens (6), the projection unit (5), the light splitting unit (3), the imaging unit (8) and the camera (9) are sequentially arranged on the same straight line; the light splitting unit (3) is used for separating incident light and reflected light, the light emitted by the light source (1) is irradiated onto the spatial light modulator (4) through the light splitting unit (3); the projection unit (5) projects the illumination light field modulated by the spatial light modulator (4) onto the surface of a target object; the reflected light of the target object reversely passes through the micropore array on the spatial light modulator (4) to form a confocal image; the imaging unit (8) images the confocal image to the camera (9).
2. The confocal microscopy system of claim 1, wherein: The light source (1) is an LED light source, and a beam shaping unit (2) is arranged between the LED light source and the light splitting unit (3).
3. The confocal microscopy system of claim 1, wherein: The light splitting unit (3) is a dichroic mirror or a polarization splitting prism.