Automatic daphnia magna breeding and breed conservation device
By designing an automated Daphnia magna breeding and seed preservation device and using a controller to uniformly control all components, the problem of existing devices being unable to automate feeding and water quality control was solved, and the automation and flexible expansion of Daphnia magna breeding was achieved.
Patent Information
- Application Number
- CN202422935741.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-28
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2034-11-28
AI Technical Summary
Existing large-scale Daphnia breeding equipment fails to comprehensively regulate feeding and water quality conditions, cannot achieve automated control, and cannot flexibly increase or decrease breeding containers according to demand.
An automated Daphnia magna breeding and seed preservation device was designed, which included a Daphnia magna breeding chamber, a water-cooled constant temperature chamber, an algae breeding chamber and a controller. The controller uniformly controlled each component to achieve automated feeding and environmental control.
The automation of the Daphnia gracilis culture process is achieved, ensuring that all indicators meet the requirements, avoiding manual operation errors, and supporting flexible expansion of culture volume and simultaneous daily culture and seed preservation.
Smart Images
Figure CN223472866U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of Daphnia magna aquaculture, specifically to an automated Daphnia magna aquaculture and breeding device. Background Technology
[0002] Daphnia magna is an arthropod belonging to the family Daphniae in the order Cladocera of the class Crustacea, and is an important component of aquatic ecosystems. Due to its short life cycle, rapid reproduction, ease of acquisition, and sensitivity to pollutants, it has become an internationally recognized standard test organism. In 1978, the U.S. Environmental Protection Agency (EPA) designated Daphnia magna as a mandatory test item in toxicity testing, establishing the first standard method for its toxicity testing. Other countries subsequently developed their own standard methods, and my country also formulated its acute toxicity determination method in 1991. The toxicity testing of Daphnia magna has gradually been widely studied globally, expanding to various types of toxicity testing standard methods, including reproductive toxicity tests, enrichment tests, and combined toxicity tests. It is widely used in chemical safety assessment, wastewater monitoring, and surface water quality control.
[0003] To ensure the stability and continued usability of the Daphnia magna used in experiments, they are typically domesticated in a laboratory. Most commercially available Daphnia magna materials are dormant eggs, which can be preserved for extended periods under specific conditions and can be retrieved for hatching and cultivation at any time. Therefore, from the perspective of experimental controllability, the in-house cultivation of dormant Daphnia magna eggs is particularly important.
[0004] Some standard methods provide cultivation conditions for Daphnia macrocarpa, clearly specifying the cultivation water and its parameters such as pH, hardness, and temperature. To achieve these specified cultivation conditions, manual cultivation is often used in laboratories. Daphnia macrocarpa is cultured in a constant-temperature, light-controlled incubator, with appropriate ambient temperature and light cycles set. It is fed regularly and the culture medium is replenished to ensure water quality meets standards. Feeding algae is done separately through aeration, with the culture medium replenished regularly to maintain algal activity.
[0005] Existing large-scale Daphnia magna cultivation equipment has the following drawbacks: 1. Existing equipment does not comprehensively consider feeding and water quality conditions, and cannot systematically regulate various parameters during the Daphnia magna cultivation process. 2. The automatic feeding function of existing equipment cannot control the water quality conditions after the algae solution is pumped into the Daphnia magna cultivation container, nor can it regulate the water temperature, thus requiring high environmental temperature and water quality. 3. Existing equipment cannot purify the water during the water circulation process, leading to excessive algae solution in the container over a long period, affecting the normal growth and reproduction of Daphnia magna. 4. Existing cultivation equipment with temperature control devices cannot perform automatic feeding; technicians still need to manually prepare the algae solution and feed the plants periodically. 5. Existing equipment is all designed as a single unit with a fixed installation method, making it impossible to add or remove cultivation containers according to actual conditions.
[0006] Therefore, how to provide a device that integrates large-scale Daphnia breeding and conservation functions, and uniformly regulates the breeding environment and feeding through a control system, is the problem that this patent urgently needs to solve. Utility Model Content
[0007] To address the aforementioned technical problems, this utility model provides an automated large-scale Daphnia aquaculture and breeding device.
[0008] To solve the above-mentioned technical problems, this utility model provides an automated large-scale Daphnia daphnia culture and preservation device. The automated large-scale Daphnia daphnia culture and preservation device includes: a large-scale Daphnia daphnia culture chamber unit, which includes at least one large-scale Daphnia daphnia culture chamber, a water-cooled constant temperature chamber, and a water chiller. The large-scale Daphnia daphnia culture chamber is connected to the water-cooled constant temperature chamber, and the water chiller is connected to the water-cooled constant temperature chamber; an algae culture chamber unit, which includes an algae culture chamber and an algae culture medium container connected to the algae culture chamber, and the algae culture chamber is connected to the large-scale Daphnia daphnia culture chamber; and a controller, which is electrically connected to the algae culture chamber unit and the large-scale Daphnia daphnia culture chamber unit respectively.
[0009] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: the large-scale Daphnia breeding chamber contains liquid, and an overflow ring is provided on the top of the liquid.
[0010] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: the outlet of the overflow ring is connected to the water-cooled constant temperature chamber through a water pump and pipeline.
[0011] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: the bottom and top of the large-scale Daphnia breeding chamber are respectively provided with barrier nets, and the barrier net at the top is located in the gap in the middle of the overflow ring.
[0012] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: the water chiller is connected to the inlet and outlet of the water-cooled constant temperature chamber through connecting pipes.
[0013] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: a lighting lamp is provided on the top of the large-scale Daphnia breeding chamber.
[0014] The automated large-scale Daphnia daphnia cultivation and preservation device provided by this utility model may also have the following feature: the algae culture medium container is connected to the algae cultivation chamber through a liquid solenoid valve and pipeline.
[0015] The automated large-scale Daphnia daphnia cultivation and preservation device provided by this utility model may also have the following feature: the algae cultivation chamber and the large-scale Daphnia daphnia cultivation chamber are connected by a liquid solenoid valve and pipeline.
[0016] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following feature: a peristaltic pump is also provided between the liquid solenoid valve and the pipeline.
[0017] The automated large-scale Daphnia breeding and preservation device provided by this utility model may also have the following features: the bottom of the water-cooled constant temperature chamber is connected to the bottom of the large-scale Daphnia breeding chamber through a pipeline, and manual valves are respectively provided at the bottom of the water-cooled constant temperature chamber and the bottom of the large-scale Daphnia breeding chamber.
[0018] The beneficial effects of this utility model are as follows:
[0019] This invention relates to an automated large-scale Daphnia daphne cultivation and preservation device, comprising a large-scale Daphnia cultivation chamber unit, an algae cultivation chamber unit, and a controller. The large-scale Daphnia cultivation chamber unit includes a large-scale Daphnia cultivation chamber, a water-cooled constant-temperature chamber, and a water chiller; the algae cultivation chamber unit includes an algae cultivation chamber and an algae culture medium container. The controller is electrically connected to both the large-scale Daphnia cultivation chamber unit and the algae cultivation chamber unit. Based on this large-scale Daphnia cultivation and preservation device, the controller, along with the large-scale Daphnia cultivation chamber unit and the algae cultivation chamber unit, provides unified control over the aforementioned components, achieving automation of large-scale Daphnia cultivation. This ensures that all indicators during the large-scale Daphnia cultivation process meet requirements and avoids errors or interference that may occur during manual operation.
[0020] In addition, the aforementioned automated Daphnia magna culture and preservation device can be applied to the preservation and storage of Daphnia magna. By connecting the algae culture chamber and the Daphnia magna culture chamber, the feeding amount of Daphnia magna can be controlled, the environmental conditions in the Daphnia magna culture chamber can be changed, and the production of dormant eggs in Daphnia magna can be promoted.
[0021] Furthermore, in this large-scale Daphnia daphnia breeding and preservation device, there is at least one large-scale Daphnia daphnia breeding chamber. The number of large-scale Daphnia daphnia breeding chambers can be increased according to production needs, thereby expanding the cultivation volume. Moreover, the feeding and cultivation conditions of each large-scale Daphnia daphnia breeding chamber can be set differently through the controller, thereby realizing the simultaneous daily breeding, propagation and preservation of large-scale Daphnia daphnia. Attached Figure Description
[0022] Figure 1 This is a schematic diagram of the automated large-scale Daphnia aquaculture and breeding device in this embodiment;
[0023] Figure 2 This is a schematic diagram of the connection between the large daphne breeding chamber and the water-cooled constant temperature chamber in this embodiment;
[0024] Figure 3 This is a schematic diagram of the water pump in this embodiment;
[0025] Figure 4 This is a schematic diagram of the connection between the algae culture chamber and the algae culture medium container in this embodiment;
[0026] Figure 5 This is a schematic diagram of the flow of water, algae, and algae culture medium in this embodiment. Detailed Implementation
[0027] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0028] The automated Daphnia magna breeding and conservation device in this embodiment is used for the breeding and conservation of Daphnia magna.
[0029] like Figures 1-4 As shown, the automated large-scale Daphnia daphnia cultivation and preservation device includes an installation frame, a large-scale Daphnia daphnia cultivation chamber unit, an algae cultivation chamber unit, and a controller.
[0030] The mounting frame 10 is a metal mounting frame with multiple mounting positions. These mounting positions are used to install large Daphnia culture chamber units and algae culture chamber units.
[0031] The large-scale daphnia culture chamber unit includes at least one large-scale daphnia culture chamber 20, a water-cooled constant temperature chamber 30, and a water chiller 40. In this embodiment, a large-scale daphnia culture chamber 20 is used as an example for detailed explanation. In other embodiments or actual production needs, the number of corresponding pipelines and large-scale daphnia culture chambers can be increased.
[0032] The bottom of the large Daphnia aquaculture chamber 20 is mounted on the mounting frame 10 via a mounting base 21. The bottom of the large Daphnia aquaculture chamber 20 extends from the mounting position on the mounting frame 10, and a first manual valve 22 is provided on the extended portion, through which the large Daphnia aquaculture chamber 20 can be manually drained. The first manual valve 22 is in the closed state during normal operation of the entire device.
[0033] The large Daphnia aquaculture chamber 20 is a transparent, inert container filled with liquid. An overflow ring 23 is provided on the upper surface of the liquid. The center of the overflow ring 23 is empty, and a barrier net is provided in this empty space to prevent the large Daphnia in the large Daphnia aquaculture chamber 20 from moving into other containers.
[0034] In addition, a water pump 24 is installed between the large Daphnia aquaculture chamber 20 and the water-cooled constant temperature chamber 30. The A and B ends of the water pump 24 are connected to the outlet 26 of the overflow ring 23 and the water-cooled constant temperature chamber 30 respectively via pipelines. By pumping the water pump 24, the liquid in the large Daphnia aquaculture chamber 20 is drawn into the water-cooled constant temperature chamber 30, achieving heat exchange between the water-cooled constant temperature chamber 30 and the liquid in the large Daphnia aquaculture chamber 20, thus maintaining a constant temperature overall.
[0035] In addition, a lighting lamp 25 is installed on the top of the large daphnia breeding chamber 20.
[0036] The water-cooled constant temperature chamber 30 is a transparent, inert container with a metal water-cooling pipe inside. The inlet 31 and outlet 32 of the water-cooling pipe are connected to the water chiller 40. The water chiller 40 controls the liquid temperature inside the water-cooling pipe to maintain a constant temperature, thereby achieving constant temperature control of the liquid inside the water-cooled constant temperature chamber.
[0037] The bottom of the water-cooled thermostatic chamber 30 is mounted on the metal frame 10 via a water-cooled mounting base 33, and its bottom extends downward from the mounting position. A second manual valve 34 is provided on one end of the extension, through which the water-cooled thermostatic chamber 30 can be manually drained. The second manual valve 34 is in the closed state during normal operation of the entire device.
[0038] In addition, the bottom of the water-cooled constant temperature chamber 30 is connected to the bottom of the large daphnia breeding chamber 20 via a connecting pipe 35, and a barrier net 36 is provided at the inlet 27 of the end of the connecting pipe 35 that connects to the large daphnia breeding chamber 20. The barrier net 36 is provided to prevent the large daphnia from moving from the large daphnia breeding chamber 20 into the water-cooled constant temperature chamber 30.
[0039] The water chiller 40 is installed on one side of the mounting frame 10 and is connected to the inlet and outlet of the water cooling pipe through pipelines.
[0040] The algae culture chamber unit includes an algae culture chamber 50 and an algae culture medium container 60, which are detachably placed on the mounting frame 10.
[0041] An algae cultivation chamber 50 is equipped with an algae lighting lamp 51 on the bottom outer side and an inlet 52 and an outlet 53 on the top.
[0042] The algae culture medium container 60 is provided with an outlet 61 at the top.
[0043] An inlet solenoid valve 70, an outlet solenoid valve 80, and a peristaltic pump 90 are installed between the algae culture chamber 50 and the algae culture medium container 60. The algae culture chamber 50 and the algae culture medium container 60 are connected through the inlet solenoid valve 70, the outlet solenoid valve 80, and the peristaltic pump 90.
[0044] The inlet solenoid valve 70 is equipped with an algae inlet 71, an algae culture medium inlet 72, and a common port 73.
[0045] The output solenoid valve 80 is equipped with an algae outlet end 81, an algae culture medium outlet end 82, and a common port 83.
[0046] The peristaltic pump 90 has a pump head 91 at its front end, which is connected to the common port 73 and the common port 83 respectively.
[0047] The controller includes a main controller 101, a water chiller controller 102, and a lighting controller 103.
[0048] The main controller 101 is located on one side of the metal frame 10, next to the water chiller 30 and the peristaltic pump 90, and is electrically connected to each of the aforementioned chambers. The main controller 101 is equipped with a touch-screen operating screen 104. Through this screen 104, parameters such as the time period and flow rate for the delivery of culture medium to the algae cultivation chamber 50 and the delivery of algae solution to the large daphnia cultivation chamber 20 are set. Furthermore, the main controller 101 is also electrically connected to the inlet solenoid valve 70, the outlet solenoid valve 80, and the peristaltic pump 90 for controlling these components. In this embodiment, the main controller 101 is a DCS controller.
[0049] The water chiller controller 102 is installed on the water chiller 40 and is used to control the target temperature and circulation speed inside the water cooling pipes.
[0050] The lighting controller 103 is installed on one side of the algae lighting lamp 51 and is electrically connected to the algae lighting lamp 51 to control the light intensity of the algae lighting lamp 51.
[0051] like Figure 5 As shown, the flow path of the algae culture medium is as follows: it flows out from the outlet 61 of the algae culture medium container 60, enters the algae culture medium inlet 72 of the inlet solenoid valve 70, enters the peristaltic pump 90 through the common port 73 of the inlet solenoid valve 70, after being circulated by the peristaltic pump 90, it enters the outlet solenoid valve 80 through the common port 83 of the outlet solenoid valve 80, and then flows out through the algae culture medium outlet 82 of the outlet solenoid valve 80, entering the algae culture chamber 50 through the inlet 51 of the algae culture chamber 50. The flow of the algae culture medium is achieved by connecting the above ports through pipelines.
[0052] The flow path of the algae is as follows: it flows out from the algae outlet 52 of the algae cultivation chamber 50, enters the algae inlet 71 of the inlet solenoid valve 70, enters the peristaltic pump 90 through the common port 73 of the inlet solenoid valve 70, after being circulated by the peristaltic pump 90, it enters the outlet solenoid valve 80 through the common port 83 of the outlet solenoid valve 80, then flows out through the algae outlet 81 of the outlet solenoid valve 80, and finally flows into the large Daphnia cultivation chamber 20. The algae culture medium is connected to each of the above ports through pipelines.
[0053] The above-mentioned device is used to achieve constant temperature treatment between the large Daphnia magna culture chamber 20 and the water-cooled constant temperature chamber 30, the flow of feeding algae between the large Daphnia magna culture chamber 20 and the algae culture chamber 50, and the flow of algae culture liquid between the algae culture chamber 50 and the algae culture liquid container 60, thereby realizing the automated cultivation and preservation of large Daphnia magna.
[0054] The automated large-scale Daphnia daphnia cultivation and preservation device according to the above embodiments includes a large-scale Daphnia daphnia cultivation chamber unit, an algae cultivation chamber unit, and a controller. The large-scale Daphnia daphnia cultivation chamber unit includes a large-scale Daphnia daphnia cultivation chamber, a water-cooled constant-temperature chamber, and a water chiller; the algae cultivation chamber unit includes an algae cultivation chamber and an algae culture medium container. The controller is electrically connected to both the large-scale Daphnia daphnia cultivation chamber unit and the algae cultivation chamber unit. Based on this large-scale Daphnia daphnia cultivation and preservation device, the controller controls the large-scale Daphnia daphnia cultivation chamber unit and the algae cultivation chamber unit, enabling unified regulation and control of the above components. This achieves automation of large-scale Daphnia daphnia cultivation, ensuring that all indicators meet requirements during the cultivation process and avoiding errors or interference that may occur during manual operation.
[0055] In addition, the aforementioned automated Daphnia magna culture and preservation device can be applied to the preservation and storage of Daphnia magna. By connecting the algae culture chamber and the Daphnia magna culture chamber, the feeding amount of Daphnia magna can be controlled, the environmental conditions in the Daphnia magna culture chamber can be changed, and the production of dormant eggs in Daphnia magna can be promoted.
[0056] Furthermore, in this large-scale Daphnia daphnia breeding and preservation device, there is at least one large-scale Daphnia daphnia breeding chamber. The number of large-scale Daphnia daphnia breeding chambers can be increased according to production needs, thereby expanding the cultivation volume. Moreover, the feeding and cultivation conditions of each large-scale Daphnia daphnia breeding chamber can be set differently through the controller, thereby realizing the simultaneous daily breeding, propagation and preservation of large-scale Daphnia daphnia.
[0057] Obviously, those skilled in the art can make various modifications and variations to this application without departing from the spirit and scope of this application. Therefore, if such modifications and variations fall within the scope of the claims of this application and their equivalents, this application also intends to include such modifications and variations.
Claims
1. An automated large-scale Daphnia aquaculture and breeding device, characterized in that, include: A large-scale Daphnia aquaculture chamber unit, comprising at least one large-scale Daphnia aquaculture chamber, a water-cooled constant-temperature chamber, and a water chiller, wherein the large-scale Daphnia aquaculture chamber is connected to the water-cooled constant-temperature chamber, and the water chiller is communicated with the water-cooled constant-temperature chamber. An algae culture chamber unit, comprising an algae culture chamber and an algae culture medium container connected to the algae culture chamber, wherein the algae culture chamber is connected to the large Daphnia culture chamber; The controller is electrically connected to the algae culture chamber unit and the large daphne culture chamber unit.
2. The automated large-scale Daphnia aquaculture and breeding device according to claim 1, characterized in that: The large daphne breeding chamber contains liquid, and an overflow ring is provided on the top of the liquid.
3. The automated large-scale Daphnia aquaculture and breeding device according to claim 2, characterized in that: The outlet of the overflow ring is connected to the water-cooled thermostatic cavity via a water pump and pipeline.
4. The automated large-scale Daphnia aquaculture and breeding device according to claim 2, characterized in that: The large daphnia aquaculture chamber is equipped with barrier nets at its bottom and top, with the top barrier net located in the gap in the middle of the overflow ring.
5. The automated large-scale Daphnia aquaculture and conservation device according to claim 1, characterized in that: The water chiller is connected to the inlet and outlet of the water-cooled constant temperature cavity via connecting pipes.
6. The automated large-scale Daphnia aquaculture and breeding device according to claim 1, characterized in that: The top of the large daphnia breeding chamber is equipped with a lighting lamp.
7. The automated large-scale Daphnia aquaculture and conservation device according to claim 1, characterized in that: The algae culture medium container is connected to the algae culture chamber via a liquid solenoid valve and pipeline.
8. The automated large-scale Daphnia aquaculture and breeding device according to claim 1, characterized in that: The algae culture chamber and the large daphnia culture chamber are connected by a liquid solenoid valve and pipelines.
9. The automated large-scale Daphnia aquaculture and breeding device according to claim 7 or 8, characterized in that: A peristaltic pump is also installed between the liquid solenoid valve and the pipeline.
10. The automated large-scale Daphnia aquaculture and breeding device according to claim 1, characterized in that: The bottom of the water-cooled constant temperature chamber is connected to the bottom of the large daphnia aquaculture chamber via a pipeline. Manual valves are respectively installed at the bottom of the water-cooled constant temperature chamber and at the bottom of the large daphnia breeding chamber.