Preparation device of stationary phase chip for enzymatic gene synthesis
By designing a preparation device for stationary phase chips for enzymatic gene synthesis and utilizing a combination of a modified tank and a sealing cover, the stability problem of small-batch preparation of stationary phase chips was solved, achieving a pollution-free and efficient preparation process.
Patent Information
- Application Number
- CN202423021775.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2034-12-09
AI Technical Summary
The existing technology lacks stable and efficient devices for preparing stationary phase chips for enzymatic gene synthesis, especially for small-batch preparation, and chemical synthesis methods have pollution and technical barriers.
Provided is a device for preparing a stationary phase chip for enzymatic gene synthesis, comprising a chip modification box and a modification box cover. The device stably clamps the substrate through the modification groove and the chip clamping groove, and cooperates with the sealing cover to provide a stable environment. Polytetrafluoroethylene or polyvinylidene fluoride material is used to ensure chemical stability, supporting small-batch stationary phase chip preparation.
It enables small-batch preparation of stationary phase chips in a stable environment, avoids substrate falling, provides convenient preparation conditions, and reduces the risk of chemical contamination.
Smart Images
Figure CN223475058U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of enzymatic gene synthesis technology in the field of synthetic biology manufacturing, and specifically relates to a device for preparing a stationary phase chip for enzymatic gene synthesis. Background Technology
[0002] With the rapid development of biotechnology in recent years, the world has moved from the molecular biology stage of utilizing nature to the synthetic biology stage of designing nature. The most critical underlying technology is template-free gene synthesis. Chemical synthesis is currently the most commonly used gene synthesis method, but due to technical limitations, this method results in shorter gene lengths, higher technical barriers, and the generation of large amounts of pollutants and VOC emissions. Therefore, it usually requires outsourcing to multiple service providers for centralized services, and convenience and confidentiality cannot be guaranteed.
[0003] Addressing the various shortcomings of chemical synthesis methods, third-generation gene synthesis technology based on enzymatic synthesis has gradually matured and entered the commercial arena. It boasts advantages such as long-fragment synthesis, virtually no technological barriers, and no chemical pollution emissions, making it a strong competitor to chemical synthesis technologies. To achieve template-free enzymatic gene synthesis, base protection techniques, such as reversible terminators, are typically used, along with specific deprotection steps. This necessitates effective separation of the gene from the solution, with anchoring it to the stationary phase surface being the most commonly employed method.
[0004] Materials that can be used as stationary phases for gene synthesis include glass, quartz, silicon wafers, and resins. Among these, glass is the most common substrate material for preparing chip stationary phases. Currently, there is no stable device on the market for preparing chip stationary phase materials. Utility Model Content
[0005] This invention provides a device for preparing stationary phase chips for enzymatic gene synthesis, aiming to provide a device for preparing small batches of stationary phase chips.
[0006] To achieve the above objectives, the technical solution adopted by this utility model is: to provide an apparatus for preparing a stationary phase chip for enzymatic gene synthesis, comprising:
[0007] A chip modification box has multiple modification slots on its upper side, and each modification slot has a chip clamping slot at its bottom. A substrate is adapted to be placed in the modification slot, and its bottom is clamped in the chip clamping slot.
[0008] A trimming box cover is placed on the chip trimming box and is adapted to seal with the chip trimming box.
[0009] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this invention, the chip clamping groove is narrowed at both ends to clamp the two ends of the substrate.
[0010] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this invention, the sidewall of the chip clamping groove and the sidewall of the modification groove are spaced apart.
[0011] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this invention, the top of the modification box cover is provided with a handle.
[0012] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this utility model, a sealing ring is provided inside the modification box cover, and the sealing ring is embedded in the modification box cover.
[0013] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this utility model, the chip modification box and the modification box cover are both made of polytetrafluoroethylene, polyvinylidene fluoride, or resin material coated with polytetrafluoroethylene.
[0014] In one possible implementation of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided by this invention, the chip modification box has five modification slots.
[0015] The beneficial effects of the apparatus for preparing stationary phase chips for enzymatic gene synthesis provided by this utility model are as follows: Compared with the prior art, the apparatus for preparing stationary phase chips for enzymatic gene synthesis provided by this utility model allows for the placement of a substrate in each modification groove during use. The substrate is stably clamped by the chip clamping groove at the bottom of the modification groove to prevent it from falling off. Furthermore, a modification box cover that seals with the chip modification box provides a stable environment for the preparation of stationary phase chips, facilitating the preparation of stationary phase chips. Since multiple modification grooves are provided on the chip modification box, a small batch of stationary phase chips can be prepared at one time. Attached Figure Description
[0016] Figure 1 A three-dimensional structural diagram of the chip modification box in the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this embodiment of the present invention;
[0017] Figure 2 A top view of the chip modification box in the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this embodiment of the present invention;
[0018] Figure 3 For along Figure 2 Cross-sectional view of the middle BB line;
[0019] Figure 4 for Figure 2 Enlarged view of part A in the image;
[0020] Figure 5 A three-dimensional structural schematic diagram of the modification box cover in the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this embodiment of the present invention;
[0021] Figure 6 A bottom view of the modification box cover in the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this embodiment of the present invention;
[0022] Figure 7 For along Figure 6 Cross-sectional view of the CC line;
[0023] Explanation of reference numerals in the attached figures:
[0024] 10. Chip trimming box; 11. Trimming slot; 12. Chip clamping slot;
[0025] 20. Decorative lid; 21. Handle; 22. Sealing ring. Detailed Implementation
[0026] To make the technical problem to be solved, the technical solution, and the beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0027] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. The following description of at least one exemplary embodiment is actually illustrative only and is in no way intended to limit this application or its application or use. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0028] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.
[0029] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values of the components and steps described in these embodiments do not limit the scope of this application. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.
[0030] In the description of this application, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is usually based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this application and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this application; the directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.
[0031] For ease of description, spatial relative terms such as "above," "over," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "above" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways, and the spatial relative descriptions used herein will be interpreted accordingly.
[0032] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be construed as limiting the scope of protection of this application.
[0033] Please also refer to Figures 1 to 7The present invention provides a device for preparing a stationary phase chip for enzymatic gene synthesis. The device includes a chip modification box 10 and a modification box cover 20. The chip modification box 10 has multiple modification grooves 11 on its upper side, and each modification groove 11 has a chip clamping groove 12 at its bottom. A substrate is adapted to be placed in the modification groove 11, and its bottom is clamped in the chip clamping groove 12. The modification box cover 20 is placed on the chip modification box 10 and is adapted to seal with the chip modification box 10.
[0034] The beneficial effects of the apparatus for preparing stationary phase chips for enzymatic gene synthesis provided by this utility model are as follows: Compared with the prior art, the apparatus for preparing stationary phase chips for enzymatic gene synthesis provided by this utility model allows for the placement of a substrate in each modification groove 11 during use. The substrate is stably clamped by the chip clamping groove 12 at the bottom of the modification groove 11 to prevent it from falling off. Furthermore, a modification box cover 20 is provided to seal with the chip modification box 10, providing a stable environment for the preparation of stationary phase chips and facilitating the preparation of stationary phase chips. Since multiple modification grooves 11 are provided on the chip modification box 10, a small batch of stationary phase chips can be prepared at one time.
[0035] like Figures 2 to 4 As shown in the embodiment of the preparation device for the stationary phase chip for enzymatic gene synthesis provided in this utility model, the chip clamping groove 12 is narrowed at both ends to clamp the two ends of the substrate.
[0036] Specifically, the trimming groove 11 is an elongated oval groove, and the chip clamping groove 12 is an elongated groove that narrows inward at both ends.
[0037] like Figure 4 As shown in a specific embodiment of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this utility model, the sidewall of the chip clamping groove 12 and the sidewall of the modification groove 11 are spaced apart so that the substrate is separated from the inner wall of the modification groove 11, so that the substrate can fully react with the solution when preparing the stationary phase chip.
[0038] like Figure 5 As shown in a specific embodiment of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this utility model, the top of the modification box cover 20 is provided with a handle 21 for easy opening and closing.
[0039] like Figure 6 and Figure 7 As shown in the embodiment of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this utility model, a sealing ring 22 is provided inside the modification box cover 20, and the sealing ring 22 is embedded in the modification box cover 20.
[0040] Specifically, an annular groove is formed on the inner side wall of the chip trimming box 10 cover. The sealing ring 22 is embedded in the annular groove and protrudes from the inner wall of the chip trimming box 10 cover. After being placed on the chip trimming box 10, it has an interference fit with the chip trimming box 10 to ensure good sealing performance. The sealing ring 22 is made of sealing materials resistant to organic solvents, such as nitrile rubber and silicone rubber.
[0041] In one specific embodiment of the apparatus for preparing a stationary phase chip for enzymatic gene synthesis provided in this utility model, the chip modification box 10 and the modification box cover 20 are both made of polytetrafluoroethylene, polyvinylidene fluoride, or resin material coated with polytetrafluoroethylene, so as to provide good chemical and physical stability and provide a stable environment for the preparation of the stationary phase chip.
[0042] like Figure 1 and Figure 2 As shown in the embodiment of the preparation device for the stationary phase chip for enzymatic gene synthesis provided in this utility model, five modification slots 11 are provided on the chip modification box 10.
[0043] Specifically, the number of trimming slots 11 can be set according to actual needs.
[0044] The process of preparing a stationary phase chip using the above-described preparation apparatus is as follows:
[0045] Place the stationary phase chip substrate into the modification groove 11 of the chip modification box 10, and hold the substrate in place through the chip slot at the bottom of the modification groove 11; add ethanol to the modification groove 11, cover the modification box with the top cover 20, and place it in an ultrasonic cleaner for ultrasonic cleaning for 5 minutes; then, replace with deionized water for cleaning twice, and ultrasonic for 5 minutes each time.
[0046] Afterwards, drain the water from the modification tank 11, add the modification solution, seal the modification box with the lid 20, and react overnight. After the reaction is complete, drain the modification solution, wash twice with ethanol and aqueous solution respectively, and then remove the stationary phase chip and dry it at 80°C.
[0047] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. An apparatus for preparing a stationary phase chip for enzymatic gene synthesis, characterized in that, include: A chip trimming box has multiple trimming slots on its upper side, and each trimming slot has a chip clamping slot at its bottom. A substrate is suitable for being placed in the trimming slot and is clamped at its bottom in the chip clamping slot. as well as A trimming box cover is placed on the chip trimming box and is adapted to seal with the chip trimming box.
2. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in claim 1, characterized in that, The chip clamping slot is narrowed at both ends to clamp the two ends of the substrate.
3. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in claim 2, characterized in that, The sidewalls of the chip clamping slot and the sidewalls of the modification slot are spaced apart.
4. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in claim 1, characterized in that, The top of the decorative box lid has a handle.
5. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in claim 1, characterized in that, The interior of the embellishment box cover is provided with a sealing ring, which is embedded in the embellishment box cover.
6. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in claim 1, characterized in that, The chip modification box and the modification box cover are both made of polytetrafluoroethylene, polyvinylidene fluoride, or resin material coated with polytetrafluoroethylene.
7. The apparatus for preparing a stationary phase chip for enzymatic gene synthesis as described in any one of claims 1-6, characterized in that, The chip modification box has five modification slots.