Reagent pipetting system

Abstract

The utility model relates to the technical field of reagent pipetting, in particular to a reagent pipetting system, which comprises a first kit, a first hose, a first multi-way pipe joint, a second hose, a first peristaltic pump, a third hose and a first shunt block which are sequentially connected, a first pinch valve is arranged on the first hose, a first reaction tank is arranged at the bottom of the first shunt block, and a second pinch valve is arranged on the second hose. The first flow dividing block can be used for uniformly dividing the reagent, and a flow inlet of the first flow dividing block is connected with a discharging end of the first peristaltic pump through a third hose; the first flow dividing block is provided with a plurality of flow outlets, and the flow of each flow outlet is the same. According to the reagent pipetting system disclosed by the utility model, the hose, the first multi-way pipe joint, the first pinch valve, the first peristaltic pump and the first shunting block are arranged, so that reagent pipetting of a plurality of hole sites on the reaction tank can be completed at one time, the working efficiency of reagent pipetting is improved, and the work of a liquid path is controlled through the peristaltic pump and the pinch valve; and the workload of reagent pipetting can be reduced.

Description

Technical Field

[0001] This utility model relates to the technical field of reagent pipetting, and more specifically, to a reagent pipetting system. Background Technology

[0002] Immunohistochemistry (IHCC) utilizes the principle of specific binding between immunological antigens and antibodies. Through a chemical reaction, a chromogenic agent labeled with the antibody is used to develop color, allowing for the identification, qualitative analysis, and relative quantification of intracellular antigens. During IHCC experiments, reagents are pipetted to transfer each reagent into the wells of each reaction vessel.

[0003] Generally, when performing immunohistochemistry experiments, there are dozens or even hundreds of wells on the reaction vessel for pipetting. When manually adding each reagent to the wells one by one during the pipetting process, not only is the repeatability of reagent pipetting high and the workload large, but it also requires pipetting several or even a dozen different reagents, which will cause the workload of reagent pipetting to increase exponentially. Manually pipetting one by one is not only a waste of human resources, but also inefficient. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of the existing technology, which involves manual pipetting of reagents one by one, which is not only wasteful of human resources but also inefficient. This invention provides a reagent pipetting system that can improve pipetting efficiency and reduce the workload of operators.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0006] A reagent pipetting system is provided, comprising a first reagent kit, a first multi-port connector, a first clamp valve, a first peristaltic pump, a first splitter block, and a first reaction tank;

[0007] The first reagent kit has a plurality of receiving cavities, and the reagents contained in each receiving cavity of the first reagent kit do not interfere with each other;

[0008] The first multi-port connector has a discharge end and several inlet ends. Each inlet end of the first multi-port connector is connected to a receiving cavity of the first reagent kit through a first flexible tube.

[0009] The first clamp valve is located on the first hose;

[0010] The first peristaltic pump is used to drive the reagent to move forward, and the feed end of the first peristaltic pump is connected to the discharge end of the first multi-port connector through the second hose;

[0011] The first flow divider can uniformly divide the reagent. The inlet of the first flow divider is connected to the outlet of the first peristaltic pump through a third hose. The first flow divider has several outlets, and each outlet has the same flow rate.

[0012] The first reaction tank is provided with a number of holes, and each hole on the first reaction tank can be aligned with one of the outlets.

[0013] In this invention, the reagent pipetting system first pipettes one reagent from a first reagent kit. The first tubing connected to the receiving cavity of the reagent is located, and the first clamp valve on the first tubing is opened. Then, the first peristaltic pump starts operating, propelling the reagent in the tubing forward. The reagent in the receiving cavity first enters the first tubing and continues forward until it enters the first multi-way connector through its inlet end. The reagent in the first multi-way connector then enters the second tubing through its outlet end. The reagent continues forward in the second tubing until it enters the first peristaltic pump through its inlet end, and then from the outlet end of the first peristaltic pump into the third tubing. The reagent in the third tubing continues forward until it enters the first distribution block through its inlet. The reagent entering the first distribution block is evenly divided into several streams by the first distribution block, and each stream flows out through its outlet into the orifice on the first reaction tank. When it is necessary to switch to different reagents for dispensing, simply close the previously opened first clamp valve, locate the first tubing connected to the cavity containing the target reagent, and open the first clamp valve on the first tubing to complete the reagent switching.

[0014] This invention's reagent pipetting system, through the arrangement of a flexible tube, a first multi-port connector, a first clamp valve, a first peristaltic pump, and a first distributor block, enables the simultaneous pipetting of reagents from multiple orifices on a reaction vessel, improving pipetting efficiency. The peristaltic pump and clamp valve control the liquid path, reducing the workload of reagent pipetting. Furthermore, the peristaltic pump can run dry; during reagent switching, the first peristaltic pump can continue operating even after the tubing is closed, eliminating the need to shut it down and preventing frequent starting and stopping that could shorten its lifespan. Using a flexible tube for liquid transport, with its larger inner diameter than a rigid tube, provides better flow, greater liquid force, and improved pipetting effect. It is also simpler to assemble and less expensive.

[0015] Furthermore, the inlet is located at the top of the first flow divider block; the outlet is located at the bottom of the first flow divider block. The first flow divider block also contains a first channel, a second channel, and a third channel connected in sequence. The inlet is connected to the first channel, and the outlet is located at the bottom of the third channel. There are two first channels, arranged in a mirror image with the axis of the inlet as the axis of symmetry. There are three third channels, two of which are connected to the two ends of the second channel, and the other is connected to the midpoint of the second channel. The inlet being located at the top of the first flow divider block and the outlet at the bottom prevents reagent accumulation within the first flow divider block. Reagent entering the first flow divider block is first divided into two streams by the two first channels. The two streams continue to flow within the two first channels until they simultaneously enter the second channel. After entering the second channel, the two streams fill the second channel and then enter the third channel. Once the third channel is filled, the reagent flows out through the outlet. Because the reagent in the first splitting block is first divided through the first channel, the reagent entering the second channel can quickly fill it, reducing the time difference between reagents entering the various third channels. Simultaneously, the reagent needs to further fill the third channels before flowing out from the outlet, further reducing the impact of the time difference between entering different third channels on reagent outflow. This ensures that the reagents in the first splitting block can flow out from the outlet almost simultaneously, guaranteeing the same reagent flow rate at each outlet. Two of the third channels are located at the beginning of the second channel, and the other is located in the middle of the second channel. That is, the inlet ends of the three third channels are equidistant from the outlet ends of the two first channels, allowing simultaneous inflow into the three third channels. This further ensures that the reagents at the three outlets are discharged simultaneously, increasing the splitting efficiency by three times.

[0016] Furthermore, the axes of the first, second, and third flow channels are all straight. When machining the flow channels within the first flow divider block, a direct machining process of drilling first and then plugging can be used, allowing the first flow divider block to be directly obtained through machining. However, when the flow channels within the first flow divider block are curved, they cannot be directly obtained through machining and require machining through mold opening and bonding. Compared to mold opening and bonding, machining has a lower processing cost.

[0017] Furthermore, the inner diameter of the inlet is larger than the inner diameter of the first flow channel, the inner diameter of the first flow channel is larger than the inner diameter of the second flow channel, the inner diameter of the second flow channel is larger than the inner diameter of the third flow channel, and the inner diameter of the third flow channel is greater than or equal to the inner diameter of the outlet. Specifically, the inner diameter of the inlet can be 5mm, the inner diameter of the first flow channel can be 3mm, the inner diameter of the second flow channel can be 2mm, and the inner diameters of the third flow channel and the outlet can be 1mm. The inner diameters of the inlet, first flow channel, second flow channel, and third flow channel within the first distribution block decrease progressively, so that the reagent flows out of the outlet in a columnar shape, avoiding poor permeability of the reagent as it moves forward within the first distribution block and preventing the reagent flowing out of the outlet from dripping out.

[0018] Furthermore, the angle between the axes of the second flow channel and the first flow channel is 20°-45°. The angle between the second flow channel and the first flow channel is 20°-45°, which allows the reagent in the inlet to be split into two streams by the two first flow channels, and the reagent in the first flow channel can smoothly enter and fill the second flow channel.

[0019] Furthermore, it also includes a second multi-port connector. Several of the first diverter blocks and the first reaction tank are provided. The second multi-port connector has one inlet end and several outlet ends. The inlet and outlet ends of the first peristaltic pump are both located on the first pump head of the first peristaltic pump. There are several first pump heads. The second flexible tube is connected to the inlet end of the second multi-port connector. Each outlet end of the second multi-port connector is connected to the first pump head via a fourth flexible tube. Each first pump head is connected to one of the first diverter blocks via a third flexible tube. A reaction tank is located below each of the first diverter blocks. Through the arrangement of the second multi-port connector, multiple first pump heads on the first peristaltic pump, multiple first diverter blocks, and first reaction tanks, the same reagent can be transferred through multiple first diverter blocks, further improving the efficiency of reagent separation.

[0020] Furthermore, the first, second, third, and fourth tubing are all transparent PTFE tubing. PTFE tubing (polytetrafluoroethylene tubing) has the advantages of being resistant to all strong acids, strong alkalis, and strong oxidizing agents, and does not react with various organic solvents. It also has the advantages of low friction, heat resistance, non-absorbency, and aging resistance. The transparent tubing allows the operator to observe the reagent's progress during sample addition, making it easier to track the reagent's path.

[0021] This invention also provides another reagent pipetting system, including a second reagent kit, a second peristaltic pump, a second reaction vessel, and a second splitter block for uniformly distributing reagents. The second reagent kit has several receiving cavities, and the reagents contained in each receiving cavity of the second reagent kit affect each other. Each receiving cavity of the second reagent kit is connected to the inlet of a second peristaltic pump through a fifth hose. Each fifth hose is provided with a second clamp valve. The outlet of each second peristaltic pump is connected to the inlet of a second splitter block through a sixth hose. The outlet of each second splitter block can be aligned with a hole on the second reaction vessel.

[0022] When the system is operating, the second clamp valve on the fifth tubing is opened. Driven by the second peristaltic pump, the reagent in the second reagent kit moves along the tubing. First, the reagent enters the fifth tubing from the receiving cavity on the second reagent kit, and then flows along the fifth tubing to the inlet of the second peristaltic pump. The reagent entering the second peristaltic pump enters the sixth tubing from the outlet of the second peristaltic pump, and then flows along the sixth tubing into the second flow divider block. The reagent entering the second flow divider block flows from the outlet of the second flow divider block into the orifice on the second reaction tank.

[0023] Each reagent in this system is pipetted using an independent tubing, which avoids the reagents from coming into contact with each other and reacting.

[0024] Furthermore, it also includes a third multi-port connector, which has an inlet end and several outlet ends. The inlet and outlet ends of the second peristaltic pump are both located on the second pump head of the second peristaltic pump. There are several second pump heads. The fifth flexible tube is connected to the inlet end of the third multi-port connector. The outlet end of each third multi-port connector is connected to the inlet end of one of the second pump heads via a seventh flexible tube. Each outlet end of the second pump head is connected to a second flow divider block. With the third multi-port connector, the same reagent can be pipetted using the third multi-port connector, multiple second peristaltic pumps, and flow dividers, further improving the pipetting efficiency of the same reagent.

[0025] Furthermore, the system also includes the aforementioned reagent pipetting system, wherein the first and second splitter blocks have the same structure; the first and second peristaltic pumps have the same structure; the second and third multi-port connectors have the same structure; the first and second clamp valves have the same structure; and the first and second reaction tanks are the same reaction tank. Including the aforementioned reagent pipetting system enables the system to simultaneously pipette multiple reagents that may or may not react with each other. Since the first and second reaction tanks are the same reaction tank, after dispensing liquid into the orifice of the reaction tank via the first splitter block, liquid can be dispensed again into the same orifice of the same reaction tank via the second splitter block. In other words, reagents in the second reagent kit can be pipetted into the orifice of the first reaction tank via the second splitter block, and reagents in the first reagent kit can also be pipetted into the orifice of the second reaction tank via the first splitter block.

[0026] Furthermore, it also includes a mounting base with several sets of mounting positions. Each mounting position houses one first diverter block and several second diverter blocks for diverting different types of reagents. Both the first and second diverter blocks are fixedly connected to the mounting base. By fixing the diverter blocks with the mounting base, only the position of the reaction tank needs to be changed during pipetting, further reducing the workload during pipetting.

[0027] Furthermore, the inner diameters of the first, second, third, fourth, fifth, sixth, and seventh tubings are the same as the inner diameter of the first flow channel. The identical inner diameter of all tubings ensures that the reagent flow rate within all tubings is the same. The identical inner diameter of the tubings to the first flow channel ensures that the reagent flow rate within the tubing is the same as the reagent flow rate within the first flow channel, and that the reagent within the tubing can flow smoothly into the first flow channel.

[0028] Furthermore, the fifth, sixth, and seventh tubing used for transporting light-protected reagents are black PTFE tubing; the fifth, sixth, and seventh tubing used for transporting non-light-protected reagents are transparent PTFE tubing. Light-protected reagents are transported using black tubing to prevent exposure to light; non-light-protected reagents are transported using transparent tubing, allowing for real-time monitoring of their movement.

[0029] Compared with the prior art, the beneficial effects of the present invention are:

[0030] The reagent pipetting system of this invention, through the arrangement of a hose, a first multi-port connector, a first clamp valve, a first peristaltic pump, and a first diverter block, can complete reagent pipetting at multiple orifices on the reaction tank in one operation, thereby improving the efficiency of reagent pipetting. The peristaltic pump and clamp valve control the operation of the liquid path, thereby reducing the workload of reagent pipetting.

[0031] The reagent pipetting system of this invention has two first flow channels and three second flow channels inside the first flow block, which can evenly divide the reagent entering the first flow block into three streams, thus improving the flow splitting efficiency by three times.

[0032] This invention also provides another reagent pipetting system. In this system, for reagents that can affect each other, each reagent has a separate pipeline consisting of a second peristaltic pump, a second flow divider, etc., to prevent different reagents from reacting before entering the second reaction tank and affecting the reagent's effectiveness. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of a first embodiment of a reagent pipetting system based on a peristaltic pump;

[0034] Figure 2 This is a schematic diagram of the structure of the first splitter block of a reagent pipetting system based on a peristaltic pump;

[0035] Figure 3 This is a schematic diagram of a reagent pipetting system based on a peristaltic pump, excluding the tubing, in Embodiment 3.

[0036] In the attached diagram: 1. First reagent kit; 2. First clamp valve; 3. First multi-port connector; 4. First peristaltic pump; 5. First flow divider; 6. First reaction tank; 501. Inlet; 502. First flow channel; 503. Second flow channel; 504. Third flow channel; 505. Outlet; 7. First tubing; 8. Second tubing; 9. Third tubing; 10. Second multi-port connector; 11. Fourth tubing; 12. Second reagent kit; 13. Second peristaltic pump; 14. Second flow divider; 15. Third multi-port connector; 16. Mounting base; 17. Second clamp valve. Detailed Implementation

[0037] The present invention will be further described below with reference to specific embodiments. The accompanying drawings are for illustrative purposes only, representing schematic diagrams rather than actual physical objects, and should not be construed as limiting the scope of this patent. To better illustrate the embodiments of the present invention, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0038] In the accompanying drawings of this utility model, the same or similar reference numerals correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0039] Example 1

[0040] This embodiment is a first embodiment of a reagent pipetting system based on a peristaltic pump, including a first reagent kit 1, a first clamp valve 2, a first multi-port connector 3, a first peristaltic pump 4, a first splitter block 5, and a first reaction tank 6. There are two first reagent kits 1, each with two receiving cavities. The four receiving cavities of the first reagent kits 1 are used to hold four reagents that do not react with each other. The first multi-port connector 3 has one outlet and four inlets. The inlet of each first multi-port connector 3 is connected to one receiving cavity of the first reagent kit 1 through a first hose 7. Each first hose 7 is equipped with a first clamp valve 2. The inlet of the first peristaltic pump 4 is connected to the outlet of the first multi-port connector 3 through a second hose 8. The inlet 501 of the first diverter block 5 is connected to the outlet of the first peristaltic pump 4 through a third hose. The first diverter block 5 has three outlets 505, each with the same flow rate. The first reaction tank 6 has several holes, each of which can be aligned with an outlet 505.

[0041] Specifically, the inlet 501 is located at the top of the first flow divider block 5; the outlet 505 is located at the bottom of the first flow divider block 5. The first flow divider block 5 also includes a first flow channel 502, a second flow channel 503, and a third flow channel 504 connected sequentially. The inlet 501 is connected to the first flow channel 502, and the outlet 505 is located at the bottom of the third flow channel 504. There are two first flow channels 502, arranged in a mirror image with the axis of the inlet 501 as the axis of symmetry. The axis of the second flow channel 503 and the first flow channel 502 forms an angle of 20°-45°. There are three third flow channels 504, two of which are connected to the two ends of the second flow channel 503, and the third flow channel 504 is connected to the midpoint of the second flow channel 503.

[0042] Specifically, the inner diameter of the inlet 501 is 5mm, the inner diameter of the first flow channel 502 is 3mm, the inner diameter of the second flow channel 503 is 2mm, and the inner diameters of the third flow channel 504 and the outlet 505 are 1mm.

[0043] Specifically, it also includes a second multi-port connector 10. The first diverter block 5 and the reaction tank 6 each have three connectors. The second multi-port connector 10 has one inlet end and three outlet ends. The inlet and outlet ends of the first peristaltic pump 4 are both located on the first pump head of the first peristaltic pump 4. There are three first pump heads. The second hose 8 is connected to the inlet end of the second multi-port connector 10. Each outlet end of the second multi-port connector 10 is connected to a first pump head via a fourth hose 11. Each fourth hose 11 is equipped with a first clamp valve 2. Figure 1 (not shown in the image), each first pump head is connected to a first diverter block 5 via a third hose, and a reaction tank 6 is provided below each first diverter block 5.

[0044] Specifically, the first hose 7, the second hose 8, the third hose 9, and the fourth hose 11 are all transparent PTFE tubes.

[0045] The working principle or process of this embodiment is as follows:

[0046] During pipetting, the reagent on the first reagent kit 1 is pipetted first. The first tubing 7 connected to the receiving cavity of the reagent is located, and the first clamp valve 2 on the first tubing 7 is opened. Then, the first peristaltic pump 4 starts working, driving the reagent in the tubing forward. The reagent in the receiving cavity first enters the first tubing 7, and then continues to advance along the first tubing 7 until it enters the first multi-port connector 3 through the inlet end. The reagent in the first multi-port connector 3 enters the second tubing 8 through the outlet end of the first multi-port connector 3. The reagent continues to advance in the second tubing 8 until it enters the second multi-port connector 10. The reagent in the second multi-port connector 10 flows out through the outlet end of the second multi-port connector 10 and flows into the three pump heads on the first peristaltic pump 4 through three fourth tubings 11. The reagent in each pump head flows out from the pump head driven by the first peristaltic pump 4 and enters the third tubing 9. The reagent in the third tubing 9 continues to advance until it enters the first diversion block 5 through the inlet 501. The reagent entering the first diversion block 5 is first divided into two streams by the two first channels 502. These two streams continue to advance within their respective channels until they simultaneously enter the second channel 503. After entering the second channel 503, the two streams fill it and then enter the third channel 504. Once the third channel 504 is full, the reagent flows out through the outlet 505 of the first diversion block 5 and enters the orifice of the first reaction tank 6. The first reaction tank 6 can slide under the first diversion block 5. The equal residence time at the outlet 505 at each orifice ensures that the amount of reagent added to each orifice is equal. When it is necessary to switch to different reagents for dispensing, simply close the first clamp valve 2, locate the first tubing 7 connected to the cavity containing the target reagent, and open the first clamp valve 2 on the first tubing 7 to complete the reagent switching.

[0047] The beneficial effects of this embodiment are as follows:

[0048] The reagent pipetting system of this embodiment, through the arrangement of tubing, first multi-port connector 3, first clamp valve 2, first peristaltic pump 4, and first diverter block 5, can complete reagent pipetting at multiple positions on the reaction tank in one operation, improving the efficiency of reagent pipetting. The operation of the liquid circuit is controlled by the peristaltic pump and clamp valve, avoiding direct manual operation and reducing the workload of reagent pipetting. Furthermore, the peristaltic pump can run dry. During reagent switching, the first peristaltic pump 4 can still operate after the pipeline is closed, without the need to shut down the first peristaltic pump 4, thus avoiding the frequent start-up and shutdown of the first peristaltic pump 4 and affecting its service life.

[0049] The inlet 501 is located at the top of the first diversion block 5, and the outlet 505 is located at the bottom of the first diversion block 5, which can prevent reagent accumulation in the first diversion block 5. The arrangement of the inlet 501, the first flow channel 502, the second flow channel 503, and the third flow channel 504 in the first diversion block 5 ensures that the flow rate of each outlet 505 is the same, and the reagent flows out of the outlet 505 in a columnar shape, avoiding the poor permeability of the reagent when it moves forward in the first diversion block 5, which would cause the reagent to flow out of the outlet 505 in a dripping shape. The arrangement of the second multi-port connector 10, the multiple pump heads on the first peristaltic pump 4, the multiple first diversion blocks 5, and the first reaction tank 6 can further improve the efficiency of reagent separation.

[0050] PTFE (polytetrafluoroethylene) tubing has the advantages of being resistant to all strong acids, strong alkalis, and strong oxidizing agents, and does not react with various organic solvents. It also features low friction, heat resistance, non-absorbency, and aging resistance. The transparent tubing allows for observation of the reagent's progress during sample addition, making it easier for operators to track the reagent's path.

[0051] Example 2

[0052] This embodiment is a second embodiment of a reagent pipetting system based on a peristaltic pump. The reagent pipetting system of this embodiment includes a second reagent kit 12, a second peristaltic pump 13, a second clamp valve 17, a second reaction tank, and a second splitter block 14 for uniformly splitting reagents. The second reagent kit 12 is provided with three receiving cavities for holding three reagents that react with each other. Each receiving cavity of the second reagent kit 12 is connected to the inlet of a second peristaltic pump 13 through a fifth hose. Each fifth hose is provided with a second clamp valve 17. The outlet of each second peristaltic pump 13 is connected to the inlet 501 of a second splitter block 14 through a sixth hose. The outlet 505 of each second splitter block 14 can be aligned with a hole on the second reaction tank.

[0053] Specifically, it also includes a third multi-port connector 15, which has one inlet end and three outlet ends. The inlet end and outlet end of the second peristaltic pump 13 are both located on the second pump head of the second peristaltic pump 13. There are three second pump heads. The fifth hose is connected to the inlet end of the third multi-port connector 15. The outlet end of each third multi-port connector 15 is connected to the inlet end of a second pump head through a seventh hose. The outlet end of each second pump head has a second diverter block 14 connected to it.

[0054] The working principle or process of this embodiment is as follows:

[0055] When the second pipetting unit is in operation, the clamp valve 2 on the fifth tubing is opened. Driven by the second peristaltic pump 13, the reagent in the second reagent kit 12 moves along the tubing. First, the reagent enters the fifth tubing from the receiving cavity on the second reagent kit 12, and then flows along the fifth tubing into the third multi-port connector 15. The reagent entering the third multi-port connector 15 is split by the connector and flows out from the three outlets of the connector. The reagent flowing out from the third multi-port connector 15 flows along the seventh tubing into the second peristaltic pump 13. Driven by the second peristaltic pump 13, the reagent entering the second peristaltic pump 13 flows from the outlet of the second peristaltic pump 13 into the sixth tubing, and then along the sixth tubing into the second diversion block 14. The reagent entering the second diversion block 14 flows from the outlet 505 of the second diversion block 14 into the orifice on the second reaction tank.

[0056] The beneficial effects of this embodiment are as follows:

[0057] For reagents that can interact with each other, each reagent has its own pipeline consisting of a second peristaltic pump 13, a second flow divider 14, etc., to prevent different reagents from reacting before entering the second reaction tank and affecting the reagent's effectiveness. The addition of a third multi-port connector 15 further improves the pipetting efficiency of the same reagent when handling reagents that react with each other.

[0058] Example 3

[0059] This embodiment is a third embodiment of a reagent pipetting system. Based on embodiment two, this embodiment further defines the structure of the pipetting system.

[0060] Specifically, it also includes a reagent pipetting system as described in Example 1, wherein the first splitter block 5 and the second splitter block 14 have the same structure; the first peristaltic pump 4 and the second peristaltic pump 13 have the same structure; the second multi-port connector 10 and the third multi-port connector 15 have the same structure; the first clamp valve 2 and the second clamp valve 17 have the same structure; and the first reaction tank 6 and the second reaction tank are the same reaction tank.

[0061] Specifically, it also includes a mounting base 16, which has several sets of mounting positions. Each mounting position has a first diversion block 5 and several second diversion blocks 14 for diverting different types of reagents. The first diversion block 5 and the second diversion block 14 are both fixedly connected to the mounting base 16.

[0062] Specifically, the fifth, sixth, and seventh tubing used for transporting light-protected reagents are black PTFE tubing; the fifth, sixth, and seventh tubing used for transporting non-light-protected reagents are transparent PTFE tubing. The inner diameter of the first tubing 7, second tubing 8, third tubing 9, fourth tubing 11, fifth tubing, sixth tubing, and seventh tubing is the same as the inner diameter of the first flow channel 502.

[0063] The beneficial effects of this embodiment are as follows:

[0064] This embodiment of the pipetting system can perform batch pipetting of reagents that react with each other, as well as reagents that do not react with each other. The mounting base 16 can fix the flow divider, and only the position of the reaction tank 6 needs to be changed during pipetting, further reducing the workload. Light-protected reagents are transported using black tubes to prevent light exposure; non-light-protected reagents are transported using transparent tubes, allowing real-time monitoring of their progress. The inner diameter of the flexible tube is the same as that of the first flow channel 502, ensuring that the reagent flow rate in the flexible tube is the same as that in the first flow channel 502, and that the reagent in the flexible tube flows smoothly into the first flow channel 502.

[0065] In the specific implementation of the above embodiments, the technical features can be combined in any non-contradictory way. For the sake of brevity, not all possible combinations of the above technical features are described. However, as long as the combination of these technical features is not contradictory, it should be considered to be within the scope of this specification.

[0066] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A reagent pipetting system, characterized in that, It includes a first reagent kit (1), a first multi-port connector (3), a first clamp valve (2), a first peristaltic pump (4), a first flow divider (5), and a first reaction tank (6); The first reagent kit (1) is provided with a plurality of receiving cavities, and the reagents contained in each receiving cavity of the first reagent kit (1) do not interfere with each other; The first multi-port connector (3) is provided with a discharge end and several inlet ends. The inlet end of each of the first multi-port connectors (3) is connected to a receiving cavity of the first reagent kit (1) through a first hose (7). The first clamp valve (2) is located on the first hose (7); The first peristaltic pump (4) is used to drive the reagent to move forward. The feed end of the first peristaltic pump (4) is connected to the discharge end of the first multi-port connector (3) through the second hose (8). The first diverter block (5) can uniformly divert the reagent. The inlet (501) of the first diverter block (5) is connected to the outlet of the first peristaltic pump (4) through the third hose (9). The first diverter block (5) has several outlets (505), and each outlet (505) has the same flow rate. The first reaction tank (6) is provided with a number of holes, and each hole on the first reaction tank (6) can be aligned with one of the outlets (505).

2. The reagent pipetting system according to claim 1, characterized in that, The inlet (501) is located at the top of the first diverter block (5); the outlet (505) is located at the bottom of the first diverter block (5). The first diverter block (5) is also provided with a first flow channel (502), a second flow channel (503) and a third flow channel (504) connected in sequence. The inlet (501) is connected to the first flow channel (502), and the outlet (505) is located at the bottom of the third flow channel (504). There are two first flow channels (502), and the two first flow channels (502) are arranged in a mirror image with the axis of the inlet (501) as the axis of symmetry. There are three third flow channels (504), of which two third flow channels (504) are connected to the two ends of the second flow channel (503) respectively, and the other third flow channel (504) is connected to the midpoint of the second flow channel (503).

3. The reagent pipetting system according to claim 2, characterized in that, The inner diameter of the inlet (501) is greater than the inner diameter of the first flow channel (502), the inner diameter of the first flow channel (502) is greater than the inner diameter of the second flow channel (503), the inner diameter of the second flow channel (503) is greater than the inner diameter of the third flow channel (504), and the inner diameter of the third flow channel (504) is greater than or equal to the inner diameter of the outlet (505).

4. The reagent pipetting system according to claim 2, characterized in that, The included angle between the axes of the second flow channel (503) and the first flow channel (502) is 20°-45°.

5. The reagent pipetting system according to claim 1, characterized in that, It also includes a second multi-port connector (10), which is provided in several of the first diversion block (5) and the first reaction tank (6). The second multi-port connector (10) is provided with a feed end and several discharge ends. The feed end and discharge end of the first peristaltic pump (4) are both provided on the first pump head of the first peristaltic pump (4). There are several first pump heads. The second hose (8) is connected to the feed end of the second multi-port connector (10). Each discharge end of the second multi-port connector (10) is connected to a first pump head through a fourth hose (11). Each first pump head is connected to a first diversion block (5) through a third hose (9). Each first diversion block (5) is provided with a first reaction tank (6) below it.

6. A reagent pipetting system, characterized in that, The system includes a second reagent kit (12), a second peristaltic pump (13), a second reaction tank, and a second diversion block (14) for uniformly diverting reagents. The second reagent kit (12) has several cavities. The reagents contained in each cavity of the second reagent kit (12) affect each other. Each cavity of the second reagent kit (12) is connected to the inlet of the second peristaltic pump (13) through a fifth hose. Each fifth hose is equipped with a second clamp valve (17). The outlet of each second peristaltic pump (13) is connected to the inlet of the second diversion block (14) through a sixth hose. The outlet of each second diversion block (14) can be aligned with a hole on the second reaction tank.

7. A reagent pipetting system according to claim 6, characterized in that, It also includes a third multi-port connector (15), which has an inlet end and several outlet ends. The inlet end and outlet end of the second peristaltic pump (13) are both located on the second pump head of the second peristaltic pump (13). There are several second pump heads. The fifth hose is connected to the inlet end of the third multi-port connector (15). The outlet end of each third multi-port connector (15) is connected to the inlet end of a second pump head through a seventh hose. The outlet end of each second pump head has a second diverter block (14) connected to it.

8. A reagent pipetting system according to claim 7, characterized in that, It also includes a reagent pipetting system as described in any one of claims 1-4, wherein the first splitter block (5) and the second splitter block (14) have the same structure; the first peristaltic pump (4) and the second peristaltic pump (13) have the same structure; the second multi-port connector (10) and the third multi-port connector (15) have the same structure; the first clamp valve (2) and the second clamp valve (17) have the same structure; and the first reaction tank (6) and the second reaction tank are the same reaction tank.

9. A reagent pipetting system according to claim 8, characterized in that, It also includes a mounting base (16), which has several sets of mounting positions. Each mounting position has a first diversion block (5) and several second diversion blocks (14) for diverting different types of reagents. The first diversion block (5) and the second diversion blocks (14) are fixedly connected to the mounting base (16).

10. A reagent pipetting system according to claim 7, characterized in that, The inner diameters of the first hose (7), the second hose (8), the third hose (9), the fourth hose (11), the fifth hose, the sixth hose, and the seventh hose are the same as the inner diameter of the first flow channel (502).

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