Simple zipper type cell scratch six-hole plate

Abstract

The utility model discloses a simple zipper type cell scratch six-hole plate which comprises a main body and a plate cover, the plate cover is matched with the top end of the main body, a culture hole is formed in the main body, a groove is formed in the bottom end of the culture hole, a filling belt is arranged in the groove in a filling mode, and the plate cover is matched with the top end of the main body. Each filling belt is provided with two extension shafts perpendicular to the filling belt, and the outer surface of each filling belt is provided with a coating layer. The filling strip disclosed by the utility model can be stably fixed, is not easy to fall off in the transportation and use processes, and does not add the trouble of plug-in displacement in the culture process for experiments; and in the pulling process, scratches can be generated by using small force.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment, specifically a simple zipper-type cell scratch six-well plate. Background Technology

[0002] The cell scratch assay is the most intuitive and commonly used experiment in biological experiments to test cell migration ability. It is favored by researchers because it requires simple consumables and takes little time to operate. However, the most difficult part of the scratch assay is how to quickly and uniformly draw a straight scratch.

[0003] In practice, we have found that manual scratching by experimenters may result in problems such as curved scratches, uneven scratch width, and damage to the underlying coating, which can lead to unsightly or even unusable experimental results. The most serious consequence is that the experimental results cannot be replicated, datasets with low intragroup variability cannot be obtained, and the expected scientific results cannot be achieved. Therefore, a convenient, quick device that can help experimenters make straight scratches is very important.

[0004] Existing auxiliary devices on the market are placement-type, which involve placing inserts with partitions in culture dishes or well plates to hinder cell growth at specific locations on the plate, simulating scratches. The inconvenience of this device is that the inserts need to be placed one by one in advance, and collisions must be prevented during the culture process to avoid displacement of the inserts. Furthermore, care must be taken to avoid slight hand tremors during operation to prevent experimental failure. Therefore, although this device can make the scratches straight, there are many potential accidents, making it inconvenient in actual use.

[0005] Although the scratching device in application number 202122684689.2 can help experimenters draw straight scratches in a realistic way, the device itself is too complicated and too large, and can only scratch one dish, making it inconvenient to use. Putting these aside, the device itself is very difficult to sterilize. Ensuring the sterility of this device requires a long time and a very complicated sterilization process, which adds to the difficulty of the experiment.

[0006] The scratching device in application number 201920134722.4 is relatively simple in design, but we found that this device does not have enough control over the gun head. We all know that "two points determine a straight line", but this device only controls the gun head at one point. As an experiment with N=2 degrees of freedom, this is far from enough, and the gun head will still deviate to the left or right. Furthermore, if the scratching force is uneven, the coating will still be damaged.

[0007] The scratching device in application number 201910906350.7 can help researchers make straight scratches, but the force cannot be controlled, and there is still a possibility of damaging the bottom coating; moreover, the device is too complicated, inconvenient to manufacture, and increases costs. Utility Model Content

[0008] The purpose of this invention is to provide a simple zipper-type cell scratch six-well plate to solve the problems mentioned in the background art.

[0009] To achieve the above objectives, this utility model provides the following technical solution: a simple zipper-type cell scratch six-well plate, comprising a main body 1 and a plate cover 2, wherein the plate cover 2 fits with the top of the main body 1, culture wells are provided inside the main body, and grooves are provided at the bottom of the culture wells, wherein the grooves are filled with filling strips, each of the filling strips is provided with two extension axes perpendicular to the filling strips, and the outer surface of the filling strips is provided with a coating to ensure normal cell growth.

[0010] Preferably, the culture wells are provided in six equidistant locations at the top of the main body.

[0011] Preferably, the groove has a depth of 20 micrometers and a width of 500 micrometers, and extends across the entire bottom surface of the culture well.

[0012] Preferably, two grooves are provided, and the grooves are filled with a filler material that is also covered with a coating material to ensure normal growth during the cell expansion period.

[0013] Preferably, the shape, depth, and width of the filling strip are the same as those of the groove, so that the filling strip can be filled into the groove.

[0014] Preferably, after the filling strip fills the inside of the groove, one side extends to the outside of the culture well.

[0015] Preferably, the position of the extension axis of the filling strip is fixed inside the groove by heat fusion, thereby preventing the filling strip from shifting when the device moves.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] 1. The appearance of this utility model is basically the same as that of a regular six-hole plate, which is in line with the usage habits of laboratory personnel;

[0018] 2. This utility model is small in size, is a disposable product, and can be pre-sterilized, making it convenient for operators to use;

[0019] 3. This utility model does not use tools such as gun tips to make scratches, but uses a zipper design to simulate scratches; this scratching method can also generate a certain stress on the cells, just like when scratching with gun tips or other tools.

[0020] 4. The filling strip of this utility model can be stably fixed and will not easily fall off during transportation and use, thus avoiding the troubles such as the displacement of the plug during the culture process; and scratches can be produced with a small force during the pulling process.

[0021] 5. This utility model is simple in design and easy to operate. No training is required to get started. The scratches are straight and will not damage the coating.

[0022] 6. The groove design of this utility model conforms to the cell size and actual experimental needs, and can produce perfect scratch experimental photos under a microscope;

[0023] 7. The design of the hot melting point in this utility model is not only for fixing the filler, but also can be used as a positioning point during shooting, without the need for additional marking by the experimental personnel. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of a simple zipper-type cell scratch six-well plate according to the present invention;

[0025] Figure 2 This is a top view of the main body of a simple zipper-type cell scratch six-well plate according to this utility model;

[0026] Figure 3 This is an enlarged schematic diagram (A) of a simple zipper-type cell scratch six-well plate according to the present invention;

[0027] Figure 4 This is an enlarged schematic diagram of a simple zipper-type cell scratch six-well plate of this utility model.

[0028] In the diagram: 1. Main body; 2. Plate cover; 3. Culture well; 4. Filler strip; 5. Groove; 6. Extension shaft. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] See also Figure 1-4This invention provides a simple zipper-type six-well cell scratching plate, comprising a main body 1 and a plate cover 2. The plate cover 2 fits into the top of the main body 1. Six culture wells 3 are opened inside the main body 1, equidistantly at the top of the main body 1. A groove 5 is opened at the bottom of the culture well 3, with a depth of 20 micrometers and a width of 500 micrometers, spanning the entire bottom surface of the culture well 3. There are two grooves 5. The grooves 5 are filled with a filler material with the same coating to ensure normal cell growth during the expansion period. The grooves 5 are filled with a filler band 4. Each filler band 4 has two extension shafts 6 perpendicular to the filler band 4. The shape, depth, and width of the filler band 4 are the same as those of the grooves 5, which facilitates the filling of the filler band 4 into the grooves 5. The outer surface of the filler band 4 has a coating to ensure normal cell growth. After the filler band 4 is filled into the grooves 5, one side extends to the outside of the culture well 2. The position of the extension shaft 6 of the filler band 4 is fixed inside the grooves 5 by heat fusion, thereby preventing the filler band 4 from shifting when the device moves.

[0031] Working principle:

[0032] 1. Spread cells at an appropriate density evenly at the bottom of culture well 3, and use an appropriate amount of culture medium to ensure normal cell growth;

[0033] 2. Placing the cover 2 smoothly on the main body 1 can prevent contamination by bacteria and other microorganisms and prevent excessive evaporation of the liquid;

[0034] 3. After the cells have grown to a suitable density, disinfect the laminar flow hood in advance, and then carefully move the device into the laminar flow hood.

[0035] 4. Open the cover 2 near the alcohol lamp to ensure aseptic operation;

[0036] 5. Pull up the extension of the filler strip 4 on one side of the main body 1, and pull up the entire filler strip smoothly and evenly. With appropriate force, the heat-melted fixing point can be separated from the bottom of the culture well 3, and then it can be thrown into the waste liquid bucket.

[0037] 6. Repeat step 5, and pull up the remaining three filler strips 4 as well, and drop them into the waste liquid bucket;

[0038] 7. Remove the existing culture medium and slowly wash well 3 two to three times with PBS;

[0039] 8. Add the culture medium again;

[0040] 9. Take photos of the scratched area at appropriate times. The original hot-melt fixing points can be used as positioning points to ensure the consistency of the photo positions and meet the requirements of scientific research.

[0041] 10. After using the device, dispose of it properly by throwing it into the trash can.

[0042] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0043] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A simple zipper-type cell scratch six-well plate, comprising a main body (1) and a plate cover (2), characterized in that: The cover (2) fits into the top of the main body (1). The main body (1) has a culture hole (3) inside. The bottom of the culture hole (3) has a groove (5). The groove (5) is filled with a filling strip (4). Each filling strip (4) has two extension shafts (6) perpendicular to the filling strip (4). The outer surface of the filling strip (4) is coated.

2. The simplified zipper-type cell-scratching six-well plate according to claim 1, characterized in that: The culture well (3) has six openings, which are equidistantly located at the top of the main body (1).

3. A simple zipper-type cell-scratching six-well plate according to claim 2, characterized in that: The groove (5) is 20 micrometers deep and 500 micrometers wide, and runs across the bottom surface of the entire culture well (3).

4. A simple zipper-type cell-scratching six-well plate according to claim 3, characterized in that: Two grooves (5) are provided, and the grooves (5) are filled with filler material that is also covered with the same covering material.

5. A simple zipper-type cell-scratching six-well plate according to claim 4, characterized in that: The filling strip (4) has the same shape, depth, and width as the groove (5).

6. A simplified zipper-type cell-scratching six-well plate according to claim 5, characterized in that: After the filling strip (4) fills the inside of the groove (5), one side extends to the outside of the culture well (3).

7. A simplified zipper-type cell-scratching six-well plate according to claim 6, characterized in that: The position of the extension shaft (6) of the filling strip (4) is fixed inside the groove (5) by heat fusion.

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