Cell disruption device and nucleic acid detection equipment

Abstract

The utility model relates to the technical field of cell disruption, and discloses a cell disruption device and nucleic acid detection equipment, and the cell disruption device comprises a sleeve, an ultrasonic module and an elastic piece. A storage cavity is formed in the sleeve, the top end of the ultrasonic module is used for making contact with external consumables and conducting ultrasonication on samples stored in the consumables, and the ultrasonic module is arranged in the storage cavity and can move relative to the sleeve under the action of the elastic piece so that the ultrasonic module and the consumables can be efficiently located and matched. And it is ensured that the ultrasonic module efficiently conducts ultrasonication. The bottom end of the sleeve is provided with the driving part, the elastic part is elastically compressed between the driving part and the bottom end of the ultrasonic part, the ultrasonic effects conducted to the consumable by the ultrasonic module are different under different pressures, the driving part pushes the elastic part to adjust the deformation amount of the elastic part, and then the ultrasonic module can bear different pressures. Therefore, the ultrasonic module can be driven to be adjusted to the optimal ultrasonication state.

Description

Technical Field

[0001] This invention belongs to the field of cell disruption technology, specifically relating to a cell disruption device and a nucleic acid detection equipment. Background Technology

[0002] In nucleic acid testing, cell disruption technology is typically used to extract specific proteins, DNA, or RNA. However, the ease of cell disruption varies among different organisms and even different parts of the same organism, requiring different methods. Nucleic acid testing instruments generally use ultrasonic transducers to completely break down cell structures without contacting the substance being extracted, releasing cell chambers from blood or other body cells for direct analysis, thus eliminating any contamination. While existing fully automated nucleic acid testing instruments incorporate ultrasonic transducers, these transducers generally suffer from complex designs and lack of adjustable ultrasonic pressure, leading to numerous inconveniences in practical use. Utility Model Content

[0003] The purpose of this invention is to provide a cell disruption device and a nucleic acid detection equipment, which has a simple structure and can solve the technical problem that the ultrasonic pressure cannot be adjusted during the cell disruption process.

[0004] To achieve the above objectives, this utility model provides a cell disruption device, which includes:

[0005] A sleeve having a storage cavity, with a driving component at the bottom end of the sleeve;

[0006] The ultrasonic module is located in the storage cavity and can move relative to the sleeve in the height direction. The top of the ultrasonic module is used to contact the external consumables and to ultrasonically break up the samples stored inside the consumables.

[0007] An elastic element is elastically compressed between the drive component and the bottom of the ultrasonic module. The drive component can move along the height direction to adjust the deformation of the elastic element.

[0008] In an embodiment of this utility model, the top end of the sleeve is provided with an end cap, and a through hole is provided on the end cap. The ultrasonic module includes an extension and a piston connected sequentially along the height direction. The piston is movably disposed inside the sleeve and its bottom is connected to an elastic element. The extension extends out from the through hole.

[0009] In an embodiment of this utility model, the ultrasonic module further includes a sealing ring fitted on the extension, wherein the outer peripheral wall of the sealing ring is in a sealing fit with the inner peripheral wall of the through hole.

[0010] In an embodiment of this utility model, the top surface of the extension has a receiving groove that is adapted to the bottom of the consumable.

[0011] In an embodiment of this utility model, an elongated hole is provided on the sleeve, which extends along the height direction of the sleeve. The ultrasonic module also includes a guide portion located on the outer periphery of the piston portion, and a guide protrusion is formed on the guide portion. The guide protrusion extends movably from the elongated hole and can move along the elongated hole under the action of an external driving force.

[0012] In an embodiment of this utility model, the guide portion and the piston portion are slidably coupled, and a limiting block is provided at the end of the piston portion away from the extension portion to limit the movement distance of the guide portion.

[0013] In an embodiment of this utility model, the ultrasonic module further includes a sleeve, which is sleeved on the piston portion and located between the guide portion and the elastic element, with the outer wall of the sleeve slidingly engaged with the inner wall surface of the sleeve.

[0014] In an embodiment of this utility model, a through hole is provided on the driving component, and the central axes of the through hole, the sleeve and the through hole are parallel.

[0015] In embodiments of this utility model, the sleeve is made of plastic.

[0016] and / or,

[0017] The outer periphery of the drive component is rotated with the inner peripheral wall of the sleeve via a thread.

[0018] In an embodiment of this utility model, a nucleic acid detection device is also proposed, including the cell disruption device described above.

[0019] Through the above technical solutions, the cell disruption device and nucleic acid detection equipment provided by the embodiments of this utility model have the following beneficial effects:

[0020] The cell disruption device in this embodiment includes a sleeve, an ultrasonic module, and an elastic element. A storage cavity is formed inside the sleeve. The top of the ultrasonic module contacts external consumables and ultrasonically disrupts the sample stored inside the consumables. The ultrasonic module is positioned within the storage cavity and can move relative to the sleeve under the action of the elastic element. When the ultrasonic module moves downward relative to the sleeve, it separates from the consumables. At this time, the ultrasonic module can be horizontally adjusted to ensure efficient positioning and engagement between the top of the ultrasonic module and the bottom of the consumables. Then, the ultrasonic module is driven upward relative to the sleeve, allowing it to re-engage with the consumables, ensuring good ultrasonic disruption effect. A driving component is located at the bottom of the sleeve. The elastic element is elastically compressed between the driving component and the bottom of the ultrasonic component. Under different pressures, the ultrasonic effect transmitted from the ultrasonic module to the consumables varies. By driving the elastic element, the deformation of the elastic element is adjusted, allowing it to transmit pressure to the ultrasonic module. The ultrasonic module withstands different pressures under different elastic element deformations, thereby determining the optimal ultrasonic disruption state of the ultrasonic module.

[0021] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0022] The accompanying drawings are provided to further illustrate the embodiments of the present invention and form part of the specification. They are used together with the following detailed description to explain the embodiments of the present invention, but do not constitute a limitation thereof. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0023] Figure 1 This is a schematic diagram of the cell disruption device according to the present invention;

[0024] Figure 2 The schematic diagram of the cell disruption device according to this utility model omits the sleeve and tube.

[0025] Figure 3 This is a cross-sectional view of the cell disruption device according to the present invention.

[0026] Description of Reference Numerals

[0027] 1. Sleeve 311 Receiving groove

[0028] 11 Storage cavity 32 Piston section

[0029] 12 End Cap 321 Limiting Block

[0030] 121 Through hole 33 Sealing ring

[0031] 13 Elongated hole; 34 Guide section

[0032] 2. Drive component 341 Guide protrusion

[0033] 21 through hole, 35 sleeve

[0034] 3. Ultrasonic module 351 connecting hole

[0035] 31 Extension 4 Elastic element Detailed Implementation

[0036] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0037] The cell disruption apparatus and nucleic acid detection equipment according to the present invention are described below with reference to the accompanying drawings.

[0038] like Figure 1 and Figure 2 As shown, in this embodiment, a cell disruption device is proposed, comprising a sleeve 1, an ultrasonic module 3, and an elastic element 4. A storage cavity 11 is formed within the sleeve 1, and a driving component 2 is disposed at the bottom end of the sleeve 1. The ultrasonic module 3 is disposed within the storage cavity 11 and is movable relative to the sleeve 1 in the height direction. The top end of the ultrasonic module 3 is used to contact external consumables and to ultrasonically disrupt the samples stored inside the consumables. The elastic element 4 is elastically compressed between the driving component 2 and the bottom of the ultrasonic module 3. The driving component 2 is movable in the height direction to adjust the deformation of the elastic element 4. It should be noted that the elastic element 4 is a spring as used in the prior art, and the ultrasonic module 3 is an ultrasonic transducer as used in the prior art.

[0039] In this embodiment, a storage cavity 11 is formed inside the sleeve 1. The top of the ultrasonic module 3 is used to contact the external consumables and to ultrasonically break up the samples stored inside the consumables. The ultrasonic module 3 is disposed in the storage cavity 11 and can move relative to the sleeve 1 under the action of the elastic member 4. When the ultrasonic module 3 moves downward relative to the sleeve 1, it can separate from the consumables. At this time, the ultrasonic module 3 can be horizontally adjusted so that the top of the ultrasonic module 3 can be efficiently positioned and engaged with the bottom of the consumables. Then, the ultrasonic module 3 is driven to move upward relative to the sleeve, so that the ultrasonic module 3 can re-engage with the consumables, ensuring that the ultrasonic module 3 has a good ultrasonic breaking effect. A driving component 2 is provided at the bottom end of the sleeve 1. The elastic member 4 is elastically compressed between the driving component 2 and the bottom end of the ultrasonic component. By moving the driving component 2 up and down in the height direction, the deformation of the elastic member 4 can be adjusted so that the ultrasonic module 3 is pressed by the elastic member 4 to achieve the optimal ultrasonic breaking state and improve the ultrasonic breaking effect. In this embodiment, the ultrasonic effect transmitted from the ultrasonic module 3 to the consumable is different under different pressures. The deformation of the elastic element 4 is adjusted by the drive component 2 pushing the elastic element 4, so that the elastic element 4 can transmit the pressure to the ultrasonic module 3. The ultrasonic module 3 bears different pressures under different deformations of the elastic element 4. By making multiple adjustments, the ultrasonic module 3 can be driven to reach the optimal ultrasonic fragmentation state.

[0040] like Figure 1 and Figure 2 As shown, in this embodiment, the top end of the sleeve 1 is provided with an end cap 12, and the end cap 12 is provided with a through hole 121. The ultrasonic module 3 includes an extension 31 and a piston 32 connected sequentially along the height direction. The piston 32 is movably disposed inside the sleeve 1 and its bottom is elastically connected to the elastic member 4. The extension 31 extends out from the through hole 121.

[0041] The end cap 12 has a through hole 121 in the middle. Preferably, the size of the extension 31 matches the size of the through hole 121, allowing the extension 31 to extend out of the through hole 121. This avoids gaps between the extension 31 and the through hole 121 due to poor fit, which could allow external debris to easily enter the storage cavity 11 and affect the normal operation of the ultrasonic module 3. The bottom of the piston 32 is elastically connected to the elastic element 4, allowing the piston 32 to respond more sensitively to the force generated during the elastic deformation of the elastic element 4. Thus, when the piston 32 moves downward to compress the elastic element 4 via the guide protrusion 341, the elastic element 4 will exert an upward force on the piston 32 when the force applied to the guide protrusion 341 disappears, thereby causing the piston 32 to move upward and positioning the extension 31 with the consumable.

[0042] Therefore, as Figure 1 and Figure 2 As shown, in this embodiment, the ultrasonic module 3 further includes a sealing ring 33 sealed on the extension 31. The outer peripheral wall of the sealing ring 33 seals against the inner peripheral wall of the through hole 121, further ensuring that external debris is not easily allowed to enter the storage cavity 11. Furthermore, because the sealing ring 33 has a certain deformation capability, under the high-frequency vibration of the ultrasonic module 3, the extension 31 can adaptively align with the consumable, thereby maximizing the energy transfer to the consumable and enabling the ultrasonic module 3 to achieve optimal ultrasonic performance. Preferably, the sealing ring 33 is a rubber sealing ring 33 as used in the prior art.

[0043] like Figure 1 and Figure 2 As shown, in this embodiment, the top surface of the extension 31 has a receiving groove 311 that adapts to the bottom of the consumable. The top of the extension 31 can be designed to conform to the shape of the consumable, which facilitates the transfer of more energy to the consumable. With the receiving groove 311, the bottom of the consumable can fully contact the top of the extension 31, improving the ultrasound conduction effect and also limiting the consumable, preventing it from detaching from the extension 31 due to poor fit between the consumable and the extension 31 during operation of the ultrasound module 3, thus affecting the normal operation of the cell disruption device.

[0044] like Figure 1 and Figure 2As shown, in this embodiment, the sleeve 1 is provided with an elongated hole 13, which extends along the height direction of the sleeve 1 and forms a one-sided opening at the bottom end of the sleeve 1. A positioning ring is also provided at the bottom end of the sleeve 1. The positioning ring is arranged around the outside of the sleeve 1 and a positioning port is provided corresponding to the one-sided opening. The positioning port and the one-sided opening are used to assist the sleeve 1 in positioning and ensure the stability of the cell disruption device. The ultrasonic module 3 also includes a guide portion 34 sleeved on the outer periphery of the piston portion 32. A guide protrusion 341 is formed on the guide portion 34. The guide protrusion 341 extends movably from the elongated hole 13. The guide protrusion 341 can move along the elongated hole 13 under the action of external driving force, thereby driving the piston portion 32 connected to the guide portion 34 to move together. By pressing down the guide protrusion 341, the piston portion 32 presses against the elastic member 4. After adjusting the position relative to the consumable, it is released. Under the action of the elastic member 4 elastic recovery, the guide protrusion 341 moves upward along the elongated hole 13. The piston portion 32 drives the extension portion 31 to move upward together, so that the extension portion 31 docks with the consumable.

[0045] Preferably, in this embodiment, two elongated holes 13 are formed on the sleeve 1, and the two elongated holes 13 are symmetrically arranged on the side wall of the sleeve 1. Similarly, a guide protrusion 341 is symmetrically provided on each side of the guide portion 34. During the process of connecting the extension portion 31 with the consumable, the guide protrusions 341 on both sides need to be pressed down simultaneously, so that the guide protrusions 341 move along the elongated holes 13, thereby driving the piston portion 32 to move downward, thereby compressing the elastic element 4 so that the elastic element 4 is in a compressed state. At this time, the extension portion 31 is not in contact with the consumable, so the position of the sleeve 1 can be adjusted horizontally so that the extension portion 31 can better cooperate with the consumable. After the adjustment is completed, the force applied to the guide protrusion 341 is released. Under the action of the elastic element 4 elastic recovery, the guide protrusion 341 moves upward along the elongated holes 13, and the piston portion 32 drives the extension portion 31 to move upward together, so that the extension portion 31 connects with the consumable. Compared to the technical solution with only one elongated hole 13 and guide protrusion 341, the technical solution with symmetrically arranged guide protrusions 341 has better adjustment stability and is less likely to cause unilateral deformation of the elastic element 4 due to unilateral pressure applied to the elastic element 4, resulting in excessive friction between the elastic element 4 and the inner wall of the sleeve 1, which would make it difficult to adjust the ultrasonic module 3.

[0046] In the first embodiment, the guide portion 34 and the piston portion 32 are integrally formed. Driving the guide protrusion 341 to move downward can drive the piston portion 32 to move downward as well, and the length of the extension portion 31 can be quickly adjusted.

[0047] like Figure 2 and Figure 3As shown, in the second embodiment, the guide portion 34 and the piston portion 32 are slidably engaged. A limiting block 321 is provided at the end of the piston portion 32 facing away from the extension portion 31 to limit the movement distance of the guide portion 34. During the downward movement of the guide protrusion 341 along the elongated hole 13, the piston portion 32 is only driven to move downward when the guide protrusion 341 abuts against the limiting block 321. This is suitable for situations requiring fine-tuning. The piston portion 32 is driven to descend only after a certain time interval following the downward pressing action of the guide protrusion 341, allowing for a certain adjustment response time. In this configuration, the piston portion 32 needs to be supported by an external component. The external component is located below the piston portion 32 and passes sequentially through the through hole 21 and the connecting hole 351 to prevent the piston portion 32 from slipping off the through hole 21 and the connecting hole 351 under gravity, thus affecting the normal operation of the ultrasonic module 3.

[0048] like Figure 2 and Figure 3 As shown, in this embodiment, the ultrasound module 3 further includes a sleeve 35, which is fitted onto the piston portion 32 and located between the guide portion 34 and the elastic element 4. The outer wall of the sleeve 35 slides against the inner wall of the sleeve 1. The sleeve 35 serves to isolate the guide portion 34 and the elastic element 4, avoiding frictional losses caused by direct contact between them. Furthermore, considering the design goal of reducing the overall weight of the cell disruption device, the sleeve 35 can preferably be made of plastic, effectively reducing weight. Additionally, a weight-reducing space is formed between the sleeve 35 and the piston portion 32, further reducing the overall weight of the cell disruption device while maintaining sufficient structural strength.

[0049] The drive component 2 has a through hole 21. The central axes of the through hole 21, the sleeve 35, and the through hole 121 are parallel, which ensures that the cell disruption device can perform cell disruption normally. Figure 3 As shown, in this embodiment, the drive component 2 is provided with a through hole 21. The central axes of the through hole 21, the sleeve 35, and the through hole 121 coincide to facilitate efficient ultrasonic fragmentation. It should be noted that the end of the sleeve 35 away from the guide part 34 is also provided with a connecting hole 351. Both the through hole 21 and the connecting hole 351 are used for connecting the power supply line to the piston part 32 to ensure that the ultrasonic module 3 can perform ultrasonic fragmentation efficiently, and that the power supply line will not affect the relative movement between the piston part 32 and the sleeve 1.

[0050] In this embodiment, the outer periphery of the driving component 2 is threadedly rotated with the inner peripheral wall of the sleeve 1. By rotating the driving component 2, the compression amount of the elastic element 4 can be adjusted, and the ultrasonic breaking effect of the ultrasonic module 3 under different compression amounts can be tested experimentally, thereby adjusting the ultrasonic module 3 to the optimal ultrasonic breaking state.

[0051] In this embodiment, the sleeve 35 is made of plastic, which is lightweight and has high structural strength while achieving good weight reduction. It can effectively transmit the pressure of the elastic element 4 without being easily crushed.

[0052] In this embodiment, a nucleic acid detection device is also proposed, including the cell disruption device described above. Since the nucleic acid detection device employs all embodiments of the cell disruption device, it also possesses all the beneficial effects of the cell disruption device, which will not be elaborated upon here.

[0053] In the assembly of the cell disruption device, the ultrasonic module 3 is first placed into the storage cavity 11, and the extension 31 extends out from the through hole 121 on the top cover 12 of the sleeve 1. The guide protrusion 341 extends out from the elongated hole 13. Then, the sleeve 35 is fitted onto the outer periphery of the piston part 32, and then an elastic element 4 with the same diameter as the sleeve 35 is added. Finally, by rotating the drive component 2, the external thread of the drive component 2 engages with the inner peripheral wall thread of the sleeve 1 to adjust the elastic element 4 to a suitable compression amount, ensuring that the elastic element 4 can drive the ultrasonic module 3 to achieve the optimal ultrasonic disruption state. The cell disruption device in this embodiment has a simple structure, is easy to disassemble, and is relatively easy to maintain.

[0054] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0055] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection, or communication; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. For those skilled in the art, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0056] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0057] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A cell disruption device, characterized in that, The cell disruption device includes: A sleeve (1) is formed with a storage cavity (11), and a driving component (2) is provided at the bottom end of the sleeve (1); An ultrasonic module (3) is located in the storage cavity (11) and can move relative to the sleeve (1) in the height direction. The top of the ultrasonic module (3) is used to contact external consumables and to ultrasonically break up the samples stored inside the consumables. An elastic element (4) is elastically compressed between the bottom of the drive component (2) and the ultrasonic module (3), and the drive component (2) is movable in the height direction to adjust the deformation of the elastic element (4).

2. The cell disruption apparatus according to claim 1, characterized in that, The top end of the sleeve (1) is provided with an end cap (12), and the end cap (12) is provided with a through hole (121). The ultrasonic module (3) includes an extension (31) and a piston (32) connected sequentially along the height direction. The piston (32) is movably disposed inside the sleeve (1) and its bottom is connected to the elastic member (4). The extension (31) extends out from the through hole (121).

3. The cell disruption apparatus according to claim 2, characterized in that, The ultrasonic module (3) also includes a sealing ring (33) that is sealed on the extension (31), and the outer peripheral wall of the sealing ring (33) is sealed to the inner peripheral wall of the through hole (121).

4. The cell disruption apparatus according to claim 2, characterized in that, The top surface of the extension (31) has a receiving groove (311) that is adapted to the bottom of the consumable.

5. The cell disruption apparatus according to claim 2, characterized in that, The sleeve (1) has an elongated hole (13) extending along the height direction of the sleeve (1). The ultrasonic module (3) also includes a guide portion (34) located on the outer periphery of the piston portion (32). A guide protrusion (341) is formed on the guide portion (34). The guide protrusion (341) extends movably from the elongated hole (13) and can move along the elongated hole (13) under the action of an external driving force.

6. The cell disruption apparatus according to claim 5, characterized in that, The guide portion (34) is slidably engaged with the piston portion (32), and a limiting block (321) is provided at the end of the piston portion (32) away from the extension portion (31) to limit the movement distance of the guide portion (34).

7. The cell disruption apparatus according to claim 6, characterized in that, The ultrasonic module (3) also includes a sleeve (35), which is sleeved on the piston part (32) and located between the guide part (34) and the elastic member (4). The outer wall of the sleeve (35) slides in contact with the inner wall of the sleeve (1).

8. The cell disruption apparatus according to claim 7, characterized in that, The drive component (2) has a through hole (21), and the central axes of the through hole (21), the sleeve (35) and the through hole (121) are parallel.

9. The cell disruption apparatus according to claim 7, characterized in that, The sleeve (35) is made of plastic. and / or, The outer periphery of the driving component (2) is threadedly rotated with the inner peripheral wall of the sleeve (1).

10. A nucleic acid detection device, characterized in that, Includes a cell disruption device according to any one of claims 1 to 9.

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