Quality control sheet of DNA quantitative analyzer

Abstract

The utility model discloses a quality control piece of a DNA quantitative analyzer, and relates to the technical field of quality control pieces, the quality control piece comprises a glass slide, a cell section arranged in the glass slide, a cover glass arranged at the top of the glass slide and used for covering the cell section, and a connecting mechanism arranged between the glass slide and the cover glass, and two sides of the bottom of the cover glass are provided with a plurality of fixing holes; according to the application, the cover glass is stuffed between the two groups of matched L-shaped frames, then tools such as a needle cylinder or an air pump are utilized to inflate the annular air bag, and in cooperation with the limiting of the limiting mechanism, the fixed block is inserted into the fixed hole after the annular air bag is inflated and expanded, so that the cover glass is quickly connected, fixed and mounted, more time is saved, and more convenience is brought to use. The annular air bag is inflated and expanded to partially fill a gap between the glass slide and the cover glass so as to seal the interior of the quality control piece, and the sealing ring can further seal the space between the glass slide and the cover glass, so that the cell slices in the quality control piece are not easy to deteriorate and damage.

Description

Technical Field

[0001] This application relates to the field of quality control sheet technology, and in particular to a quality control sheet for a DNA quantification analyzer. Background Technology

[0002] A cellular DNA quantification system first prepares human cells into liquid-based thin-layer cell slides, stains the cell nuclei for DNA, mounts the slides, illuminates the cells with light, measures the integrated optical density of the cell nuclei, sorts the cells based on the integrated optical density, analyzes the distribution patterns, and, in conjunction with system settings, quantifies and classifies the DNA in the tested cells. The device used in a cellular DNA quantification system to measure the integrated optical density of cell nuclei and quantify and classify cellular DNA can be called a cellular DNA quantification analyzer.

[0003] To ensure the proper functioning of the cell DNA quantification analyzer, the equipment requires regular testing and calibration. Generally, manufacturers use standard microscopic calibration slides for size calibration and quality control slides (pig liver slides, mouse liver slides) for optical density measurement and DNA quantification testing and calibration.

[0004] Patent CN210834391U discloses "a quality control slide for a standardized cell DNA quantification analyzer, comprising a glass slide and a slicing groove, wherein a cell slice is movably connected to the inner wall of the slicing groove, and a coverslip is movably connected to the inner wall of the slicing groove. This invention can effectively improve the sealing performance of the quality control slide, prevent damage to the quality control slide, and ensure the accuracy of the cell DNA quantification analyzer in detecting the quality control slide, as well as effectively guarantee the detection effect of the cell DNA quantification analyzer in detecting the quality control slide."

[0005] Regarding the aforementioned related technologies, the inventors believe that when installing the cover glass, it is necessary to first engage the first and second half-screws on both sides of the cover glass, and then rotate multiple nuts to connect the engaged first and second half-screws. However, rotating multiple nuts back and forth during the installation process is time-consuming and inconvenient, and therefore needs to be improved. Utility Model Content

[0006] To address the aforementioned issues, this application provides a quality control strip for a DNA quantification analyzer.

[0007] The quality control strip for a DNA quantification analyzer provided in this application adopts the following technical solution:

[0008] A quality control slide for a DNA quantitative analyzer includes a glass slide, a cell slice disposed within the glass slide, a coverslip disposed on top of the glass slide for covering the cell slice, and a connecting mechanism between the glass slide and the coverslip. The coverslip has several fixing holes on both sides of its bottom. The connecting mechanism includes two sets of L-shaped frames fixedly disposed on top of the glass slide for allowing the coverslip to slide and limiting the positions of both ends of the coverslip, an annular air bladder disposed within the glass slide, and a fixing block fixedly disposed on top of the annular air bladder for insertion into the fixing holes after the annular air bladder is inflated. The glass slide has a limiting mechanism for limiting the position of the fixing block, and the coverslip has a main air tube for inflating and deflating the annular air bladder.

[0009] By adopting the above technical solution, the coverslip is inserted between two matching L-shaped frames, and then the annular airbag is inflated using a syringe or air pump. With the limiting mechanism in place, the inflated annular airbag will insert the fixing block into the fixing hole, thereby quickly connecting, fixing and installing the coverslip, which is more time-saving and convenient.

[0010] Preferably, the top of the slide has a slicing groove, the cell slice is movably disposed in the slicing groove of the slide, and the bottom end of the annular airbag is fixedly connected to the slicing groove of the slide.

[0011] By adopting the above technical solution, the slide can accommodate the cell slices through the slicing groove, and the coverslip can cover the cell slices after being closed with the slide.

[0012] Preferably, the top side of the annular airbag is fixedly provided with a plurality of connecting plates that abut against the bottom of the coverslip, the fixing block is fixedly connected to the bottom of the connecting plates, the top of the annular airbag abuts against the bottom of the coverslip, and the limiting mechanism includes a plurality of limiting holes opened in the slicing groove and a limiting rod slidably connected in the limiting holes of the slide, the top end of the limiting rod being fixedly connected to the bottom of the connecting plate.

[0013] By adopting the above technical solution, the limiting rod that slides in the limiting hole can limit the connecting plate and other components, so that the connecting plate and the fixed block can only move up and down.

[0014] Preferably, the top end of the main air tube extends through and to the outside of the glass slide, and a one-way valve for one-way air intake is fixedly provided at the top end of the main air tube. A bronchus for deflating the annular air bladder is fixedly provided at the end of the main air tube near the annular air bladder. A miniature valve is fixedly installed inside the bronchus. A tension member for pulling the fixing block away from the fixing hole is fixed between the top and bottom of the inner wall of the annular air bladder when the annular air bladder is deflated.

[0015] By adopting the above technical solution, the one-way valve in the main air tube allows staff to quickly and unidirectionally inflate the annular airbag. The annular airbag can be deflated using the bronchus and micro valves. After deflation, the fixing block will disengage from the fixing hole under the tension of the tensioning component, thereby causing the coverslip to detach from the fixing.

[0016] Preferably, a sealing ring for sealing the connection between the slide and the coverslip is fixed in the slicing groove of the slide, and the sealing ring is located between the cell slice and the annular air bladder.

[0017] By adopting the above technical solution, the annular airbag and the sealing ring can seal between the slide and the coverslip, improve the sealing effect inside the quality control slide, and make the cell slices inside the quality control slide less prone to deterioration and damage.

[0018] In summary, this application includes at least one of the following beneficial technical effects:

[0019] 1. By inserting the coverslip between two matching L-shaped frames, and then using a syringe or air pump to inflate the annular airbag, combined with the limiting mechanism, the inflated annular airbag will insert the fixing block into the fixing hole, thereby quickly connecting, fixing and installing the coverslip, which is more time-saving and convenient. In addition, the inflation of the annular airbag can partially fill the gap between the slide and the coverslip, thereby sealing the quality control slide.

[0020] 2. The one-way valve in the main endotracheal tube allows for convenient and rapid one-way inflation of the annular airbag. The airbag can be deflated using the bronchus and micro-valve. After deflation, the fixing block disengages from the fixing hole under the tension of the tensioning component, thus releasing the coverslip. The entire process of disassembling the coverslip is quick and easy, as the micro-valve allows for deflation and removal from the L-shaped frame. The sealing ring further seals the slide and coverslip, improving the sealing effect within the quality control slide and preventing cell sections from deteriorating or being damaged. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of a quality control strip for a DNA quantification analyzer according to an embodiment of this application;

[0022] Figure 2 This is a front cross-sectional view of the overall structure of a quality control strip for a DNA quantification analyzer according to an embodiment of this application;

[0023] Figure 3 The embodiments of this application mainly embody Figure 1 A magnified view of the structure in the middle;

[0024] Figure 4 The embodiments of this application mainly embody Figure 2Enlarged view of the B-structure;

[0025] Figure reference numerals: 1. Glass slide; 11. Slicing groove; 12. Limiting hole; 2. Cell section; 3. Coverslip; 31. Fixing hole; 4. L-shaped frame; 41. Annular air bladder; 42. Connecting plate; 43. Fixing block; 44. Limiting rod; 45. Pulling element; 5. Main air tube; 51. One-way valve; 52. Bronchial tube; 53. Miniature valve; 6. Sealing ring. Detailed Implementation

[0026] The following is combined with Figure 1-Figure 4 This application will be described in further detail.

[0027] This application discloses a quality control strip for a DNA quantification analyzer.

[0028] A quality control strip for a DNA quantification analyzer, as referenced Figure 1-2 The device includes a glass slide 1, a cell slice 2, a coverslip, and a connecting mechanism. The top of the glass slide 1 has a slicing groove 11, and the cell slice 2 is movably disposed within the slicing groove 11 of the glass slide 1. The glass slide 1 can accommodate the cell slice 2 through the slicing groove 11. The coverslip 3 is disposed on top of the glass slide 1. When the coverslip 3 is closed with the glass slide 1, it can cover the cell slice 2. The cell slice 2 is an animal slice such as a pig liver slice or a mouse liver slice used for the calibration of a DNA quantitative analyzer. The slicing groove 11 is filled with physiological saline, which can ensure the biological activity of the cell slice 2. The glass slide 1 may have an optical density area, an integrated optical density area, and a barcode information area for verification. The optical density area, the integrated optical density area, and the barcode information area are public technologies and will not be described in detail here.

[0029] Reference Figure 2 and Figure 4 The coverslip 3 has several fixing holes 31 on both sides of its bottom. The connecting mechanism includes two sets of L-shaped frames 4 fixed on the top of the slide 1 for sliding the coverslip 3 and limiting the two ends of the coverslip 3, an annular air bladder 41 inside the slide 1, and a fixing block 43 fixed on the top of the annular air bladder 41 for insertion into the fixing hole 31 after the annular air bladder 41 is inflated. The bottom end of the annular air bladder 41 is fixedly connected to the slicing groove 11 of the slide 1. The bottom end of the annular air bladder 41 is fixed inside the slide 1, while the top end of the annular air bladder 41 can deform and expand inside the slide 1. After the annular air bladder 41 is inflated, it can insert the fixing block 43 into the fixing hole 31, thereby connecting the slide 1 and the coverslip 3.

[0030] Reference Figure 2 and Figure 4A number of connecting plates 42 are fixedly provided on one side of the top of the annular airbag 41, which abut against the bottom of the cover glass 3. The fixing block 43 is fixedly connected to the bottom of the connecting plate 42. The top of the annular airbag 41 abuts against the bottom of the cover glass 3. The connecting plate 42 can connect the fixing block 43 and the annular airbag 41. The annular airbag 41 can be inflated to partially fill the gap between the slide 1 and the cover glass 3, thereby sealing the inside of the quality control slide.

[0031] Reference Figure 2 and Figure 4 The slide 1 is provided with a limiting mechanism for limiting the fixing block 43. The limiting mechanism includes several limiting holes 12 opened in the slicing groove 11 and a limiting rod 44 slidably connected in the limiting holes 12 of the slide 1. The top end of the limiting rod 44 is fixedly connected to the bottom of the connecting plate 42. The limiting rod 44 slidably provided in the limiting holes 12 can limit the connecting plate 42 and other components, so that the connecting plate 42 and the fixing block 43 can only move up and down. After the fixing block 43 is inserted into the fixing hole 31, it can connect and fix the cover glass 3 in conjunction with the limiting of the limiting rod 44.

[0032] Reference Figure 1-3 The coverslip 3 is provided with a main air tube 5 for inflating and deflating the annular air bladder 41. The top end of the main air tube 5 passes through and extends to the outside of the slide 1. A one-way valve 51 for one-way air intake is fixed at the top end of the main air tube 5. The one-way valve 51 is located at the end of the main air tube 5 away from the annular air bladder 41. The staff can use tools such as syringes or air pumps to inflate and deflate the main air tube 5. The gas in the main air tube 5 will flow into the annular air bladder 41 under the one-way restriction of the one-way valve 51, thereby inflating the annular air bladder 41 and causing it to expand.

[0033] Reference Figure 2-4 The main air tube 5 is fixedly provided with a bronchus 52 for deflating the annular air bag 41 at one end. A miniature valve 53 is fixedly installed inside the bronchus 52. The miniature valve 53 can be a miniature valve, such as the miniature cross valve used in medical urine bags. After the staff opens the miniature valve 53, the air in the annular air bag 41 will be released along the main air tube 5 and the bronchus 52, thereby deflating the annular air bag 41.

[0034] Reference Figure 2-4 A tension member 45 is fixed between the top and bottom of the inner wall of the annular airbag 41 to pull the fixing block 43 away from the fixing hole 31 when the annular airbag 41 is deflated. The tension member 45 can be a tension spring or a rubber band. After the annular airbag 41 is deflated, under the tension of the tension member 45, the connecting plate 42 and the fixing block 43 at one end of the annular airbag 41 will move downward, thereby causing the fixing block 43 to disengage from the fixing hole 31, and thus causing the cover glass 3 to disengage from the fixing.

[0035] Reference Figure 2 and Figure 4 A sealing ring 6 is fixedly provided in the slicing groove 11 of the slide 1 to seal the connection between the slide 1 and the coverslip 3. The sealing ring 6 is located between the cell slice 2 and the annular air bladder 41. The sealing ring 6 and the annular air bladder 41 can seal the connection between the slide 1 and the coverslip 3, improve the sealing effect in the quality control slide, and prevent the cell slice 2 in the quality control slide from deteriorating and being damaged. In addition, the sealing ring 6 can block the cell slice 2 and the annular air bladder 41, so that the physiological saline at the cell slice 2 is not easy to flow into the annular air bladder 41.

[0036] The implementation principle of a quality control strip for a DNA quantification analyzer in this application is as follows:

[0037] When it is necessary to install the coverslip 3, the staff can slide the coverslip 3 between the two matching L-shaped frames 4 so that the coverslip 3 can cover the cell slice 2 in the slide 1.

[0038] Then, staff can use tools such as syringes or air pumps to inflate and inflate the main air tube 5. The gas in the main air tube 5 will flow into the annular air bladder 41 under the one-way restriction of the one-way valve 51, inflating the annular air bladder 41 and causing it to expand. With the limiting rod 44 and the connection of the connecting plate 42, the inflated annular air bladder 41 can insert the fixing block 43 into the fixing hole 31, thereby connecting the slide 1 and the cover glass 3, and installing and fixing the cover glass 3.

[0039] Furthermore, the inflation of the annular airbag 41 can partially fill the gap between the slide 1 and the coverslip 3. Combined with the sealing ring 6, it can improve the sealing effect inside the quality control slide, making the cell slices 2 inside the quality control slide less prone to deterioration and damage.

[0040] When it is necessary to remove and replace the coverslip 3, the staff can open the micro valve 53 to release the air in the annular airbag 41 along the main air pipe 5 and the branch air pipe 52, thereby deflating the annular airbag 41. After the annular airbag 41 is deflated, under the pulling force of the tension member 45, the connecting plate 42 and the fixing block 43 at one end of the annular airbag 41 will move downward, thereby causing the fixing block 43 to disengage from the fixing hole 31, and thus the coverslip 3 to be detached from the fixing. Then the staff can pull the coverslip 3 out of the L-shaped frame 4 to complete the disassembly process of the coverslip 3, which is convenient for replacement.

[0041] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A quality control slide for a DNA quantification analyzer, comprising a glass slide (1), a cell slice (2) disposed within the glass slide (1), a coverslip (3) disposed on top of the glass slide (1) for covering the cell slice (2), and a connecting mechanism disposed between the glass slide (1) and the coverslip (3), characterized in that: The cover glass (3) has several fixing holes (31) on both sides of its bottom. The connecting mechanism includes two sets of L-shaped frames (4) fixed on the top of the slide (1) for sliding the cover glass (3) and limiting the two ends of the cover glass (3), an annular airbag (41) in the slide (1), and a fixing block (43) fixed on the top of the annular airbag (41) for fitting into the fixing hole (31) after the annular airbag (41) is inflated. The slide (1) has a limiting mechanism for limiting the fixing block (43), and the cover glass (3) has a main air tube (5) for inflating and deflating the annular airbag (41).

2. The quality control strip for a DNA quantification analyzer according to claim 1, characterized in that: The top of the slide (1) is provided with a slicing groove (11), the cell slice (2) is movably disposed in the slicing groove (11) of the slide (1), and the bottom end of the annular air bladder (41) is fixedly connected in the slicing groove (11) of the slide (1).

3. The quality control strip for a DNA quantification analyzer according to claim 2, characterized in that: The annular airbag (41) has several connecting plates (42) fixedly abutting against the bottom of the cover glass (3) on one side of its top. The fixing block (43) is fixedly connected to the bottom of the connecting plate (42), and the top of the annular airbag (41) abuts against the bottom of the cover glass (3).

4. The quality control strip for a DNA quantification analyzer according to claim 3, characterized in that: The limiting mechanism includes a plurality of limiting holes (12) opened in the slicing groove (11) and a limiting rod (44) slidably connected in the limiting holes (12) of the glass slide (1). The top end of the limiting rod (44) is fixedly connected to the bottom of the connecting plate (42).

5. The quality control strip for a DNA quantification analyzer according to claim 4, characterized in that: The top end of the main air pipe (5) extends through and to the outside of the glass slide (1), and a one-way valve (51) for one-way air intake is fixedly provided at the top end of the main air pipe (5).

6. The quality control strip for a DNA quantification analyzer according to claim 5, characterized in that: The main air tube (5) is fixedly provided with a bronchus (52) for deflating the annular airbag (41) at one end. A miniature valve (53) is fixedly installed inside the bronchus (52).

7. The quality control strip for a DNA quantification analyzer according to claim 6, characterized in that: A tension member (45) is fixed between the top and bottom of the inner wall of the annular airbag (41) for pulling the fixing block (43) away from the fixing hole (31) when the annular airbag (41) is deflated.

8. The quality control strip for a DNA quantification analyzer according to claim 2, characterized in that: A sealing ring (6) is fixedly provided in the slicing groove (11) of the slide (1) for sealing the connection between the slide (1) and the coverslip (3). The sealing ring (6) is located between the cell slice (2) and the annular air bladder (41).

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