Liquid-phase fluorescence detector of LED light source

Abstract

The utility model relates to a liquid-phase fluorescence detector of an LED light source, which solves the problems of reducing the cost of an ultraviolet light source and increasing the detection accuracy of ultraviolet fluorescent substances. According to the technical scheme, a light source light path with an LED detection light source and a detection light path with a fluorescence detection sensor are arranged on a detection machine body. A sample cup used for detection is characterized in that the cup bottom is a plane, and the cup body is a conical light-transmitting cup body. The light source light path corresponds to the cup bottom of the sample cup, and the fluorescence detection sensor obtains fluorescence of the cup bottom of the sample cup through the detection light path. The utility model has the advantages of wide range of detected fluorescent substances, high detection efficiency, accurate and reliable detection results, mutual verification, low cost and the like, and can be widely used for medical detection, chemical substance analysis and the like.

Description

Technical Field

[0001] This invention relates to an instrument for detecting and analyzing chemical substances based on fluorescent labeling, particularly a liquid fluorescence detector for medical testing. Background Technology

[0002] Existing fluorescence detectors (such as dry fluorescence analyzers, real-time nucleic acid amplification analyzers, and isothermal nucleic acid amplification analyzers) all use laser lamps to excite fluorescence analyzers with wavelengths in the blue and green light bands. Due to the low cost of blue and green laser lamps, they are widely used in existing nucleic acid amplification analyzers and isothermal nucleic acid amplification analyzers; however, these fluorescence detectors can only detect fluorescent substances in the blue and green light bands. The high cost and large size of ultraviolet (UV) laser lamps limit their large-scale application in fluorescence analyzers.

[0003] Although LED UV lamps offer advantages such as a wide wavelength range and low cost, even with an OD6 filter, some emitted light (detected light) at wavelengths such as 422nm still remains after filtration. This unfiltered detected light exhibits significant reflection during UV testing, leading to inaccurate results. For example, when using a dry fluorescence analyzer with 365nm UV light to test card-type reagents, the UV light illuminates not only the fluorescent material but also the card itself, causing significant deviations in the test values ​​due to reflections from the card. In existing technologies, real-time nucleic acid amplification analyzers and isothermal nucleic acid amplification analyzers emit light from the top onto the sample cup. Because the liquid level varies within the sample cup, the UV light cannot be consistently focused on the liquid surface, making testing impossible. Furthermore, existing sample cups are made of PP material, which strongly reflects UV light, resulting in significant interference. Additionally, the fluorescent material is located at the bottom, with a large distance between it and the light source, further contributing to reflection interference and inaccurate test results. In summary, existing publicly available liquid chromatography-fluorescence (LC-fluorescence) detectors are unable to detect ultraviolet fluorescent substances (including fluorescent whitening agents 28, AMCA, AMCA-X, ABQ, DAPI, Acridline, Alexa, Fluor350, Fluor405, BFP, Calcein, Violet, Calcofluor White, Dansyl, DIFUM, FAST BLUE, Flourspheres Blue, hoechst33258, hoechst33342, Pacific Blue, PBFI, Y66H, True Blue, etc.), significantly limiting the scope of application of LC-fluorescence detectors. Utility Model Content

[0004] To address the technical challenges of reducing the cost of ultraviolet light sources, increasing the detection of ultraviolet fluorescent substances, improving the accuracy of detection, and enhancing the degree and efficiency of automated detection in existing fluorescence detectors, this utility model discloses a liquid phase fluorescence detector with an LED light source.

[0005] The technical solution adopted by this utility model to achieve the purpose of the invention is: a liquid phase fluorescence detector with LED light source, including: a fluorescence detection part and a sample cup.

[0006] The fluorescence detection section includes: a detection body, an LED detection light source, a light source flat lens, a sampling end flat lens, a detection end flat lens, a light source filter, a detection filter, a dichroic mirror, and a fluorescence detection sensor;

[0007] The detection body is composed of an LED detection light source, a light source flat lens, a light source filter, a dichroic mirror, and a sampling end flat lens to form a light source optical path, and a sampling end flat lens, a dichroic mirror, a detection filter, and a detection end flat lens to form a detection optical path. The light receiving end of the fluorescence detection sensor is connected to the detection end flat lens of the detection optical path.

[0008] The fluorescence detection section is equipped with a sample cup mounting bracket on the detection body. The sample cup is mounted on the sample cup mounting bracket, and the bottom of the sample cup corresponds to the sampling end flat lens in the light source optical path.

[0009] The sample cup has a flat bottom and a conical, light-transmitting body.

[0010] The advantages of this invention are: it can detect a wide range of fluorescent substances, has high detection efficiency and accurate and reliable detection results, and is low in cost. It can be widely used in medical testing and analysis of chemical substances.

[0011] The present invention will now be described in detail with reference to the accompanying drawings. Attached Figure Description

[0012] Appendix Figure 1 This is a schematic diagram of the structure of this utility model.

[0013] Appendix Figure 2 For the appendix Figure 1 A schematic diagram of the structure along direction A.

[0014] In the attached diagram: 1. Detection head body, 2. LED detection light source, 3-1. Light source flat lens, 3-2. Sampling end flat lens, 3-3. Detection end flat lens, 4-1. Light source filter, 4-2. Detection filter, 5. Dichroic mirror, 6. Fluorescence detection sensor, 7. Sample cup, 8. Sample cup mounting bracket. Detailed Implementation

[0015] A liquid fluorescence detector with an LED light source includes: a fluorescence detection part and a sample cup.

[0016] The fluorescence detection section includes: a detection body 1, an LED detection light source 2, a light source flat lens 3-1, a sampling end flat lens 3-2, a detection end flat lens 3-3, a light source filter 4-1, a detection filter 4-2, a dichroic mirror 5, and a fluorescence detection sensor 6;

[0017] The detection body 1 is composed of an LED detection light source 2, a light source flat lens 3-1, a light source filter 4-1, a dichroic mirror 5, and a sampling end flat lens 3-2 forming a light source optical path, and a sampling end flat lens 3-2, a dichroic mirror 5, a detection filter 4-2, and a detection end flat lens 3-3 forming a detection optical path. The light receiving end of the fluorescence detection sensor 6 is connected to the detection end flat lens 3-3 of the detection optical path.

[0018] The fluorescence detection part is provided with a sample cup mounting frame 8 on the detection body 1, and the sample cup 7 is mounted on the sample cup mounting frame 8. The bottom of the sample cup 7 corresponds to the sampling end flat lens 3-2 in the light source optical path.

[0019] The sample cup 7 is a cup with a flat bottom and a conical, light-transmitting body.

[0020] In use, the sampling end flat lens 3-2 corresponds to the bottom of the sample cup 7 on the sample cup mounting bracket 8, and the detection end flat lens 3-3 corresponds to the light receiving end of the fluorescence detection sensor 4.

[0021] The LED detection light source 2 is an ultraviolet lamp. The ultraviolet light emitted is transmitted through the light source flat lens 3-1, filtered by the light source filter 4-1, separated by the dichroic mirror 5, and transmitted through the sampling end flat lens 3-2 to emit ultraviolet light with a wavelength of 365nm, which then irradiates the fluorescent material in the sample cup 7.

[0022] The fluorescent substance in sample cup 7, after absorbing ultraviolet light, emits strong light that is transmitted through sampling end lens 3-2, separated by dichroic mirror 5, filtered by detection filter 4-2, and transmitted through detection end lens 3-3 before being received by the fluorescence detection sensor 6. This completes the detection of the fluorescent substance in the liquid within sample cup 7.

[0023] In this embodiment of the present invention, two sets of light source optical path, detection optical path and fluorescence detection sensor 6 are provided on the detection body 1 of the fluorescence detection part.

[0024] The two sets of light source optical paths and detection optical paths can emit light of different wavelengths (such as ultraviolet light with a wavelength of 365nm or blue light with a wavelength of 470nm). The two sets of optical paths can sequentially perform two detections on the same sample cup 7, which can expand the range of fluorescent substances that can be detected or mutually verify the detection results of fluorescent substances.

Claims

1. A liquid fluorescence detector with an LED light source, comprising: The fluorescence detection unit and sample cup are characterized by: The fluorescence detection part includes: a detection body (1), an LED detection light source (2), a light source flat lens (3-1), a sampling end flat lens (3-2), a detection end flat lens (3-3), a light source filter (4-1), a detection filter (4-2), a dichroic mirror (5), and a fluorescence detection sensor (6). The detection body (1) is composed of an LED detection light source (2), a light source flat lens (3-1), a light source filter (4-1), a dichroic mirror (5), and a sampling end flat lens (3-2). The detection light path is composed of a sampling end flat lens (3-2), a dichroic mirror (5), a detection filter (4-2), and a detection end flat lens (3-3). The light receiving end of the fluorescence detection sensor (6) is connected to the detection end flat lens (3-3) of the detection light path. The fluorescence detection part is provided with a sample cup mounting bracket (8) on the detection body (1), and the sample cup (7) is mounted on the sample cup mounting bracket (8). The bottom of the sample cup (7) corresponds to the sampling end flat lens (3-2) in the light source optical path. The sample cup (7) is a cup with a flat bottom and a conical, light-transmitting body.

2. The liquid fluorescence detector with an LED light source according to claim 1, characterized in that: On the detection body (1) of the fluorescence detection part, two sets of light source optical path, detection optical path and fluorescence detection sensor (6) are set.

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