Freezing cell resuscitation device for multi-model cryopreservation tubes

Abstract

The utility model discloses a frozen cell resuscitation device for multi-model cryopreservation tubes. The frozen cell resuscitation device comprises a jacking pop-up assembly, a heating clamping assembly and a driving guide assembly, the heating clamping assembly comprises a cryopreservation tube base, a short tube adapter, a first clamp and a second clamp, the first clamp and the second clamp are oppositely arranged, semi-tubular heating modules are arranged on the opposite sides of the first clamp and the second clamp correspondingly, a clamping channel is formed between the two heating modules, and the heating clamping assembly is in driving connection with the driving guide assembly. The driving guide assembly is used for driving the first clamp and the second clamp to get close to each other, so that the cryopreservation tube is clamped and fixed in a clamping channel between the two heating modules; the jacking and popping assembly comprises a fixing base and a popping mechanism, the cryopreservation tube base is installed on the top of the popping mechanism, and the short tube adapter is used for being placed in a clamping channel above the cryopreservation tube base so as to achieve height compensation of the short type cryopreservation tube. The cryopreservation tube fixing device can be matched with cryopreservation tubes with different diameters and length specifications, and is low in economic cost.

Description

Technical Field

[0001] This utility model relates to the field of cell thawing and resuscitation technology, specifically to a frozen cell resuscitation device with multiple types of cryopreservation tubes. Background Technology

[0002] Cell resuscitation refers to the process of thawing cells frozen in liquid nitrogen or at -70°C and then reculturing them to resume growth. Most existing techniques involve manual water bath heating for resuscitation. Specifically, the cryovial containing the cell sample is removed from the liquid nitrogen or ultra-low temperature freezer and quickly transferred to a 37°C water bath for heating. While heating, the cryovial is rotated to ensure even heating of the cell solution. Resuscitation is complete when only a small amount of ice crystals remain in the cryovial. The cryovial is then removed for subsequent centrifugation, transfer, and culture.

[0003] However, water bath heating has the following drawbacks: the biological samples in the cryopreservation tubes are highly susceptible to contamination; the water bath cannot be used as part of the aseptic process, and real-time monitoring and recording of tube information and status are impossible during operation, thus limiting its use in GMP or clinical environments; it is non-standardized, non-intelligent, and cannot be integrated into automated cell culture equipment. To address these shortcomings of traditional water bath resuscitation, dry heat resuscitation devices have been introduced to the market, but existing dry heat resuscitation devices still have some limitations:

[0004] (1) Since cryopreservation tubes of different specifications have different diameters and lengths (heights), each existing dry heat cell resuscitation instrument can only be adapted to a single specification of cryopreservation tube. Users who use cryopreservation tubes of multiple specifications need to purchase different models of resuscitation instruments, which increases user costs.

[0005] (2) It is slow to preheat and requires a long heating time to reach the preset recovery temperature;

[0006] (3) During use, the cryopreservation tube and the heating module may not fit together, resulting in uneven heating, low heating efficiency, and affecting the recovery effect. Utility Model Content

[0007] The present invention aims to provide a frozen cell revival device with multiple models of cryopreservation tubes to solve the above-mentioned technical problems.

[0008] To achieve the above objectives, the technical solution of this utility model is as follows: a frozen cell revival device for multiple types of cryovials, comprising a lifting and ejection assembly, a heating clamping assembly, and a driving and guiding assembly that cooperate with each other, wherein a temperature control assembly is provided inside the heating clamping assembly;

[0009] The heating clamping assembly includes a cryopreservation tube base, a short tube adapter, and a first clamp and a second clamp arranged opposite to each other. Semi-tubular heating modules are respectively arranged on opposite sides of the first clamp and the second clamp, and a clamping channel is formed between the two heating modules. The heating clamping assembly is driven to connect with a drive guide assembly. The drive guide assembly is used to drive the first clamp and the second clamp to move closer to each other so that the cryopreservation tube is clamped and fixed in the clamping channel between the two heating modules.

[0010] The lifting and ejection assembly includes a fixed base and an ejection mechanism that is flexibly and vertically movable on the fixed base. The ejection mechanism is located below the clamping channel. The cryotube base is installed on top of the ejection mechanism, and the top of the cryotube base is located at the bottom of the clamping channel. The short tube adapter is used to be placed in the clamping channel above the cryotube base to achieve height compensation for short cryotubes.

[0011] Preferably, the fixed base has a through hole, and an guide block and a fixed frame are fixedly installed on the upper and lower sides of the fixed base corresponding to the through hole, respectively. An electromagnet is installed in the fixed frame, and the pop-out mechanism is vertically and vertically installed in the guide block. A lifting elastic element is installed between the pop-out mechanism and the electromagnet.

[0012] Preferably, a first side plate and a second side plate are respectively provided on the fixed seats on both sides of the guide block. Vertical sliding holes are opened on the first side plate and the second side plate respectively. The pop-out mechanism includes a lifting rod and a guide cross bar that are cross-shaped. The two ends of the guide cross bar are respectively slidably disposed on the vertical sliding holes of the first side plate and the second side plate. A limiting sleeve is provided on the guide cross bar between the first side plate and the lifting rod and between the second side plate and the lifting rod.

[0013] Preferably, the first clamp is fixedly installed on one side of the first side plate, and the second clamp is located on the side of the first clamp away from the first side plate. The drive guide assembly includes a drive mechanism, which includes a support base and a motor fixedly installed on the support base. A gear is installed on the drive end of the motor, and a rack is installed on one end of the second clamp. The gear and rack are engaged in a transmission.

[0014] Preferably, the drive guide assembly further includes a guide mechanism, which includes a guide support fixedly mounted on a fixed base and a bushing fixedly mounted on the guide support. The bushing is located on the side of the second clamp away from the first clamp, and a guide module that cooperates with the guide of the second clamp is installed in the bushing for moving and guiding the second clamp.

[0015] Preferably, the guide module includes a guide shaft, a guide shaft bushing, and a limiting spring that are assembled together. The fixed seat is mounted on the guide shaft bushing, and the limiting spring is mounted outside the guide shaft. The two ends of the limiting spring abut against the second clamp and the guide shaft bushing, respectively. A limiting piece for limiting the guide shaft is provided on the side of the bushing seat 308 away from the second clamp.

[0016] Preferably, the heating module includes a semi-tubular heating element and a thermally conductive silicone pad, with the thermally conductive silicone pad disposed on the inner side of the heating element.

[0017] Preferably, the heating element is a polyimide heating element.

[0018] Preferably, the temperature control component includes an infrared temperature sensor and an NTC temperature control device, which are respectively connected to the heating module, with the infrared temperature sensor installed in the first fixture.

[0019] This utility model has the following beneficial effects:

[0020] (1) This device can be adapted to cryopreservation tubes of different diameters and lengths (heights) without replacing the internal parts. It is convenient to pick up and put down, and easy for users to use. It also reduces the cost of instruments and equipment for users.

[0021] (2) This device has a fast preheating speed, which improves user efficiency, and can maintain a stable temperature during the recovery process to ensure recovery stability.

[0022] (3) Using this device for cell resuscitation can avoid the problem of air gaps caused by non-fitting between cryopreservation tubes and heating modules, which reduces heat transfer efficiency and ensures resuscitation efficiency and cell survival rate. Attached Figure Description

[0023] Figure 1 This is an assembly diagram of an embodiment of the present invention at one angle.

[0024] Figure 2 This is an assembly diagram of an embodiment of the present invention from another angle.

[0025] Figure 3 This is an exploded view of the heating clamping assembly according to an embodiment of the present invention.

[0026] Figure 4 This is a schematic diagram of the temperature control component according to an embodiment of the present invention.

[0027] Figure 5 This is a schematic diagram of the drive guide component of an embodiment of the present invention at an angle.

[0028] Figure 6 This is a schematic diagram of the drive guide component of an embodiment of the present invention from another angle.

[0029] Figure 7 This is a schematic diagram of the lifting and pop-out assembly of an embodiment of the present invention at one angle.

[0030] Figure 8 This is a schematic diagram of the lifting and pop-out assembly of an embodiment of the present invention from another angle.

[0031] Figure labels: 1 Heating clamp assembly, 11 Heating element, 12 Thermal conductive silicone sheet, 13 First clamp, 14 Second clamp, 15 First side plate, 16 Cryopreservation tube base, 17 Short tube adapter; 2 Temperature control assembly, 21 Infrared temperature sensor, 22 NTC temperature control device; 3 Drive guide assembly, 301 Support base, 302 Motor, 303 Rack, 304 Gear, 305 Guide shaft, 306 Guide shaft bushing, 307 Limiting spring, 308 Guide shaft bushing seat, 309 Guide shaft limiting piece, 310 Guide support; 4 Lifting and ejection assembly, 41 Fixing frame, 42 Electromagnet, 43 Guide block, 44 Fixing base, 45 Guide crossbar, 46 Limiting sleeve, 47 Lifting elastic element, 48 Second side plate, 49 Lifting rod. Detailed Implementation

[0032] To further illustrate the various embodiments, the present invention provides accompanying drawings. These drawings are part of the disclosure of the present invention and are mainly used to illustrate the embodiments, and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these drawings, those skilled in the art should be able to understand other possible implementations and the advantages of the present invention. Components in the drawings are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0033] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0034] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0035] See Figure 1-8As shown, as an embodiment of the present invention, a cryopreservation device for multiple types of cryovials is provided, including a lifting and pop-out assembly 4, a heating clamping assembly 1 and a driving and guiding assembly 3 that cooperate with each other. The heating clamping assembly 1 is provided with a temperature control assembly 2 inside.

[0036] The heating clamping assembly 1 includes a cryopreservation tube base 16, a short tube adapter 17, and a first clamp 13 and a second clamp 14 arranged opposite to each other. The first clamp 13 and the second clamp 14 are made of high-temperature resistant insulation material and have good insulation performance. Semi-tubular heating modules are respectively arranged on opposite sides of the first clamp 13 and the second clamp 14, and a clamping channel is formed between the two heating modules. The heating clamping assembly 1 is driven and connected to the drive guide assembly 3. The drive guide assembly 3 is used to drive the first clamp 13 and the second clamp 14 to move closer to each other so that the cryopreservation tube is clamped and fixed in the clamping channel between the two heating modules.

[0037] The lifting and ejection assembly 4 includes a fixed base 44 and an ejection mechanism that is flexibly and vertically movable on the fixed base 44. The ejection mechanism is located below the clamping channel. The cryopreservation tube base 16 is installed on top of the ejection mechanism, and the top of the cryopreservation tube base 16 is located at the bottom of the clamping channel. The short tube adapter 17 is used to be placed in the clamping channel above the cryopreservation tube base 16 to achieve height compensation for short cryopreservation tubes.

[0038] This invention relates to a cryopreservation device for multiple cryovial models. The device preheats the cryovial to the required temperature and maintains it. The cryovial to be thawed is then inserted into the clamping channel of the heated clamping assembly 1 and pressed into place. The drive guide assembly 3 automatically closes to clamp and fit cryovials of different diameters and heights. The temperature control assembly 2 detects a cryovial at a low temperature and initiates the thaw program. After thaw, the cryovial is automatically ejected by an ejector mechanism, preventing overheating and solving the problem of misfitting between the cryovial and the heating device, thus improving thaw efficiency. This invention provides a multi-model cryopreservation cell thawing device that can adapt to cryopreservation tubes of different diameters and lengths (heights). By driving the guide assembly 3, the first clamp 13 and the second clamp 14 are driven to approach each other, so that the cryopreservation tube is clamped and fixed in the clamping channel between the two heating modules, thereby realizing the adaptation and clamping of cryopreservation tubes of different diameters. When it is necessary to thaw a short cryopreservation tube, the short tube adapter 17 is first inserted into the clamping channel placed above the cryopreservation tube base 16, and then the short cryopreservation tube is placed into the clamping channel to cooperate with the short tube adapter 17 for height compensation. When it is necessary to thaw a long cryopreservation tube, the short tube adapter 17 is removed from the clamping channel, and then the long cryopreservation tube is directly placed into the clamping channel to cooperate with the cryopreservation tube base 16.

[0039] The temperature control component 2 can monitor and record the temperature of the heating module and the cryovial temperature in real time throughout the entire process of thawing frozen cells in the cryovial. It also has an intelligent adjustment function to stably maintain the temperature during thawing and monitor the final temperature of the cryovial. The lifting and ejection component 4 is controlled by a self-developed program. After the temperature control component 2 detects that the cryovial has reached the final thawing temperature, it automatically ejects the cryovial, keeping it away from the heat source to avoid overheating during thawing and ensuring the survival rate and safety of the thawed cells.

[0040] In this embodiment, a through hole is provided on the fixed base 44, and an guide block 43 and a fixed frame 41 are fixedly installed on the upper and lower sides of the fixed base 44 corresponding to the through hole, respectively. An electromagnet 42 is installed in the fixed frame 41, and the pop-out mechanism is elastically and vertically arranged in the guide block 43. A lifting elastic element 47, specifically a lifting spring, is installed between the pop-out mechanism and the electromagnet 42.

[0041] In this embodiment, a first side plate 15 and a second side plate 48 are respectively provided on the fixed seats 44 on both sides of the guide block 43. Vertical sliding holes are respectively provided on the first side plate 15 and the second side plate 48. The pop-out mechanism includes a lifting rod 49 and a guide cross rod 45 that are cross-shaped. The two ends of the guide cross rod 45 are respectively slidably disposed on the vertical sliding holes of the first side plate 15 and the second side plate 48. A limiting sleeve 46 is provided on the guide cross rod 45 between the first side plate 15 and the lifting rod 49 and between the second side plate 48 and the lifting rod 49 to prevent the pop-out mechanism from swaying left and right. This setting ensures the accuracy and stability of the pop-out mechanism when it moves up and down elastically.

[0042] In this embodiment, the first clamp 13 is fixedly installed on one side of the first side plate 15, and the second clamp 14 is located on the side of the first clamp 13 away from the first side plate 15. The drive guide assembly 3 includes a drive mechanism, which includes a support base 301 and a motor 302 fixedly installed on the support base 301. A gear 304 is installed on the drive end of the motor 302, and a rack 303 is installed on one end of the second clamp 14. The gear 304 and the rack 303 are driven together. The second clamp 14 is driven by the motor 302 to move towards the first clamp 13 to achieve closure or to move away from the first clamp 13 to achieve opening. This causes the clamping channel to shrink to clamp the cryopreservation tube or expand to loosen the cryopreservation tube. This structure makes the clamping or loosening process of the cryopreservation tube more stable and reliable.

[0043] In this embodiment, the drive guide assembly 3 further includes a guide mechanism, which includes a guide support 310 and a bushing seat 308 fixedly installed on the guide support 310. The bushing seat 308 is located on the side of the second clamp 14 away from the first clamp 13. A guide module that guides and cooperates with the second clamp 14 is installed in the bushing seat 308 for guiding the movement of the second clamp 14. Specifically, the guide module includes a guide shaft 305, a guide shaft bushing 306, and a limiting spring 307 that are assembled with each other. The fixed seat 44 is installed on the guide shaft bushing 306, and the limiting spring 307 is installed outside the guide shaft 305. The two ends of the limiting spring 307 abut against the second clamp 14 and the guide shaft bushing 306, respectively. A limiting piece 309 for limiting the guide shaft 305 is provided on the side of the bushing seat 308 away from the second clamp 14.

[0044] In this embodiment, the heating module includes a semi-tubular heating element 11 and a thermally conductive silicone sheet 12. The thermally conductive silicone sheet 12 is disposed inside the heating element 11, thereby achieving a tight wrap around cryopreservation tubes of different specifications, ensuring uniform heating of the cryopreservation tubes. The thermally conductive silicone sheet has an excellent thermal conductivity. The heating element 11 is a polyimide heating element, which has the performance of rapid heating and uniform surface temperature, which can accelerate the preheating time, solve the problem of excessively long preheating time, and improve user efficiency and cell resuscitation stability.

[0045] In this embodiment, the temperature control component 2 includes an infrared temperature sensor 21 and an NTC temperature control device 22, which are respectively connected to the heating module. The infrared temperature sensor 21 is installed in the first clamp 13, and the NTC temperature control device 22 is located between the flexible heating element 11 and the thermally conductive silicone sheet 12. Through the independently developed control program, the NTC temperature control device 22 can be used to control the recovery temperature of the flexible heating element 11, and the infrared temperature sensor 21 can be used to detect the temperature of the cell sample in the cryopreservation tube in real time.

[0046] The cell thawing process of the cryovials of this invention includes the following steps: Connect the power supply and turn on the system. The system runs an automatic preheating program, and the heating element 11 starts heating. The NTC temperature control device 22 monitors the temperature of the heating element 11 in real time. When the temperature of the heating element 11 reaches the set temperature, the heating element 11 stops heating, and simultaneously, the motor 302 drives the heating clamping assembly 1 to the open state. After automatic preheating is completed, the next procedure begins. The cryovial to be thawed is placed into the clamping channel of the heating clamping assembly 1. The cryovial is compressed and lifted out of the lifting elastic element 47 in the assembly 4, causing the electromagnet 42 to attract the cryovial base 16 and the limiting sleeve 46, thus fixing the cryovial in the heating clamping assembly 1. Temperature sensor 21 in temperature control component 2 detects the temperature of cryovial in real time. The system runs the resuscitation program. Motor 302 drives heating clamping component 1 to be in the closed state. At the same time, heating element 11 starts heating until temperature sensor 21 detects that the surface temperature of cryovial reaches the resuscitation endpoint set temperature. Heating element 11 stops heating, motor 302 drives heating clamping component 1 to be in the open state, and ejection mechanism moves upward under the action of lifting elastic element 47 to eject cryovial from clamping channel. Cell resuscitation is completed.

[0047] This utility model has the following advantages:

[0048] (1) This device can be adapted to cryopreservation tubes of different diameters and lengths (heights) without replacing the internal parts. It is convenient to pick up and put down, and easy for users to use. It also reduces the cost of instruments and equipment for users.

[0049] (2) This device has a fast preheating speed, which improves user efficiency, and can maintain a stable temperature during the recovery process to ensure recovery stability.

[0050] (3) Using this device for cell resuscitation can avoid the problem of air gaps caused by non-fitting between cryopreservation tubes and heating modules, which reduces heat transfer efficiency and ensures resuscitation efficiency and cell survival rate.

[0051] Although the present invention has been specifically shown and described in conjunction with preferred embodiments, those skilled in the art should understand that any changes in form and detail made to the present invention without departing from the spirit and scope of the present invention as defined in the appended claims fall within the protection scope of the present invention.

Claims

1. A cryopreservation device for multiple types of cryovials, characterized in that: It includes a lifting and ejection assembly, a heated clamping assembly, and a drive and guide assembly that work together. The heated clamping assembly is equipped with a temperature control component. The heating clamping assembly includes a cryopreservation tube base, a short tube adapter, and a first clamp and a second clamp arranged opposite to each other. Semi-tubular heating modules are respectively arranged on opposite sides of the first clamp and the second clamp, and a clamping channel is formed between the two heating modules. The heating clamping assembly is driven to connect with a drive guide assembly. The drive guide assembly is used to drive the first clamp and the second clamp to move closer to each other so that the cryopreservation tube is clamped and fixed in the clamping channel between the two heating modules. The lifting and ejection assembly includes a fixed base and an ejection mechanism that is flexibly and vertically movable on the fixed base. The ejection mechanism is located below the clamping channel. The cryotube base is installed on top of the ejection mechanism, and the top of the cryotube base is located at the bottom of the clamping channel. The short tube adapter is used to be placed in the clamping channel above the cryotube base to achieve height compensation for short cryotubes.

2. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 1, characterized in that: The fixed base has a through hole, and an guide block and a fixed frame are fixedly installed on the upper and lower sides of the fixed base corresponding to the through hole, respectively. An electromagnet is installed in the fixed frame. The pop-out mechanism is raised and lowered in the guide block, and a lifting elastic element is installed between the pop-out mechanism and the electromagnet.

3. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 1, characterized in that: The fixed seats on both sides of the guide block are respectively provided with a first side plate and a second side plate. The first side plate and the second side plate are respectively provided with vertical sliding holes. The pop-out mechanism includes a lifting rod and a guide cross bar that are cross-shaped. The two ends of the guide cross bar are respectively slidably mounted on the vertical sliding holes of the first side plate and the second side plate. The guide cross bar between the first side plate and the lifting rod, and between the second side plate and the lifting rod is provided with a limiting sleeve.

4. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 3, characterized in that: The first clamp is fixedly installed on one side of the first side plate, and the second clamp is located on the side of the first clamp away from the first side plate. The drive guide assembly includes a drive mechanism, which includes a support base and a motor fixedly installed on the support base. A gear is installed on the drive end of the motor, and a rack is installed on one end of the second clamp. The gear and rack are engaged in a transmission.

5. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 1, characterized in that: The drive guide assembly also includes a guide mechanism, which includes a guide support fixedly mounted on a fixed base and a bushing fixedly mounted on the guide support. The bushing is located on the side of the second clamp away from the first clamp. A guide module that cooperates with the guide of the second clamp is installed in the bushing for moving and guiding the second clamp.

6. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 5, characterized in that: The guide module includes a guide shaft, a guide shaft bushing, and a limiting spring that are assembled together. The fixed seat is mounted on the guide shaft bushing, and the limiting spring is mounted on the outside of the guide shaft. The two ends of the limiting spring abut against the second clamp and the guide shaft bushing, respectively. A limiting piece for limiting the guide shaft is provided on the side of the bushing seat away from the second clamp.

7. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 1, characterized in that: The heating module includes a semi-tubular heating element and a thermally conductive silicone pad, with the thermally conductive silicone pad located inside the heating element.

8. The cryopreservation device for multiple cryovial models according to claim 7, characterized in that: The heating element is a polyimide heating element.

9. The cryopreservation cell resuscitation device with multiple cryovial models according to claim 1, characterized in that: The temperature control component includes an infrared temperature sensor and an NTC temperature control device, which are respectively connected to the heating module. The infrared temperature sensor is installed in the first fixture.

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