Nematode specimen manufacturing device

Abstract

The utility model relates to the technical field of plant quarantine, in particular to a nematode specimen manufacturing device. Comprising a primary dewatering box and a deep dewatering box which share a shell, the primary dewatering box comprises a primary dewatering bin with an upper cover and a first timing temperature controller connected with the primary dewatering bin, a dewatering liquid pool is placed on the lower portion in the primary dewatering bin, and a nematode box containing area is arranged on the upper portion in the primary dewatering bin; the deep dewatering box comprises a deep dewatering bin with an upper cover and a second timing temperature controller connected with the deep dewatering bin, a nematode box containing area is arranged in the deep dewatering bin, and the nematode box comprises a plurality of nematode vessels and vent holes formed in the peripheries of the nematode vessels. The nematode specimen processing device is reasonable in structure, ingenious in design and high in integration level, overcomes the defects that nematode bodies are difficult to dehydrate and time and labor are wasted in the nematode specimen manufacturing process, achieves one-stop processing operation in the nematode specimen manufacturing process, and improves the quarantine work efficiency and quality.

Description

Technical Field

[0001] This utility model relates to the field of plant quarantine technology, and in particular to a device for preparing nematode specimens. Background Technology

[0002] In plant nematode quarantine, it is necessary to prepare permanent nematode slides, i.e., nematode specimens. The most important and difficult part of nematode specimen preparation is the dehydration process. Currently, the method for nematode dehydration uses a simple homemade device. The nematodes are first placed in a cell culture dish containing a homemade dehydration solution, then placed in a sealed plastic box containing 96% ethanol. Due to the liquid, a small object is needed to elevate the cell culture dish. The entire device is then placed in an incubator for initial dehydration. After 12 hours at a constant temperature of 40 degrees Celsius, the solution in the cell culture dish is replaced, and the device is placed in the incubator again for more than 12 hours at 40 degrees Celsius. This homemade device is prone to instability and spillage of the nematode solution. Furthermore, it requires a dedicated area in the incubator to control temperature and time, leaving the remaining incubator space unused and wasting space. The entire process is also cumbersome. Therefore, there are currently no commercially available devices or instruments specifically for nematode dehydration and specimen preparation; a dedicated nematode specimen preparation device remains a gap in the market. Utility Model Content

[0003] This invention addresses the problems existing in the prior art by providing a nematode specimen preparation device that requires little operator experience, is simple to operate, and has a high nematode collection rate.

[0004] The technical solution adopted by this utility model to achieve the above-mentioned objective is as follows: a nematode specimen preparation device, characterized in that it includes a primary dehydration chamber and a deep dehydration chamber, both sharing a single shell. The primary dehydration chamber includes a primary dehydration compartment with a top cover and a timer and temperature controller connected thereto. The lower part of the primary dehydration compartment contains a dehydration liquid pool, and the upper part contains a nematode box placement area. The deep dehydration chamber includes a deep dehydration compartment with a top cover and a timer and temperature controller connected thereto. The interior of the deep dehydration compartment is a nematode box placement area, and the nematode box includes multiple nematode containers and ventilation holes opened around them.

[0005] The dehydration tank is placed inside the primary dehydration chamber via a limiting seat. The dehydration tank contains a 96% ethanol solution. The nematode box is placed inside the primary dehydration chamber via a slot.

[0006] The nematode box is made of acrylic and is placed inside the chamber via a limiting seat located below the deep dehydration chamber. The vent is elongated.

[0007] The temperature adjustment range of the timer temperature controller 1 and the timer temperature controller 2 is 0-65℃, and the time adjustment range is 0-18h. The heating rods of both are located below the dehydration chamber.

[0008] The outer insulation layer of the shell is connected.

[0009] This utility model has a reasonable structure, ingenious design, and high integration. It makes up for the shortcomings of difficult and time-consuming dehydration of nematode bodies in the preparation of nematode specimens, realizes one-stop processing operation in the preparation of nematode specimens, and improves the efficiency and quality of quarantine work. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the structure of this utility model;

[0011] Figure 2 for Figure 1 Sectional view of AA;

[0012] Figure 3 for Figure 1 BB section view;

[0013] In the diagram: 1. Dehydration tank; 2. Nematode box; 3. Top cover; 4. Timer temperature controller one; 5. Timer temperature controller two; 6. Heating rod; 7. Shell; 8. Insulation layer; 9. Nematode container; 10. Ventilation hole. Detailed Implementation

[0014] Example

[0015] like Figures 1-3 The apparatus for preparing nematode specimens shown includes a primary dehydration chamber and a deep dehydration chamber, both sharing a single housing 7. The primary dehydration chamber includes a primary dehydration compartment with a top cover 3 and a timer and temperature controller 4 connected thereto. The lower part of the primary dehydration compartment contains a dehydration liquid pool 1, and the upper part is a nematode box placement area. The deep dehydration chamber includes a deep dehydration compartment with a top cover 3 and a timer and temperature controller 5 connected thereto. The interior of the deep dehydration compartment is a nematode box placement area. The nematode box 2 includes multiple nematode containers 9 and ventilation holes 10 around them.

[0016] The dehydration tank 1 is placed inside the primary dehydration chamber via a limiting seat. The dehydration tank 1 contains a 96% ethanol solution. The nematode box 2 is placed inside the primary dehydration chamber via a slot.

[0017] The nematode box 2 is made of acrylic and is placed inside the chamber through a limiting seat located below the deep dehydration chamber. The vent 10 is elongated.

[0018] Both the timer temperature controller 4 and the timer temperature controller 5 have a timer end prompt function. The temperature adjustment range of both is 0-65℃ and the time adjustment range is 0-18h. The heating rods 6 of both are located below the dehydration chamber.

[0019] The outer insulation layer 8 is connected to the shell 7.

[0020] The method of using this invention is as follows: A 96% ethanol solution is placed in the dehydration tank 1. Nematode container 9 in the nematode box 2 contains nematode formaldehyde fixation solution and an ethanol-glycerol mixture. First, place the nematode box 2 above the dehydration tank 1, and start the timer temperature controller 4 for 12 hours at a set temperature of 40°C. After the timer expires, place the nematode box 2 into the deep dehydration chamber, add the ethanol-glycerol mixture to the nematode container 9, and start the timer temperature controller 5 for 12 hours at a set temperature of 40°C. After the timer expires, remove the nematodes from the nematode box 2 in the deep dehydration chamber. At this point, the nematodes are dehydrated and fixed and can be used for nematode specimen preparation.

Claims

1. A nematode specimen preparation apparatus, characterized in that, The device includes a primary dehydration chamber and a deep dehydration chamber, both sharing a single housing. The primary dehydration chamber includes a primary dehydration compartment with a top cover and a timer and temperature controller connected thereto. Inside the primary dehydration compartment, a dehydration liquid pool is placed at the bottom, and a nematode box placement area is located at the top. The deep dehydration chamber includes a deep dehydration compartment with a top cover and a timer and temperature controller connected thereto. Inside the deep dehydration compartment, a nematode box placement area is located, and the nematode box includes multiple nematode containers and ventilation holes around them.

2. The nematode specimen preparation apparatus according to claim 1, characterized in that, The dehydration tank is placed inside the primary dehydration chamber via a limiting seat. The dehydration tank contains a 96% ethanol solution. The nematode box is placed inside the primary dehydration chamber via a slot.

3. The nematode specimen preparation apparatus according to claim 2, characterized in that, The nematode box is made of acrylic and is placed inside the chamber via a limiting seat located below the deep dehydration chamber. The vent is elongated.

4. A nematode specimen preparation apparatus according to any one of claims 1 to 3, characterized in that, The temperature adjustment range of the timer temperature controller 1 and the timer temperature controller 2 is 0-65℃, and the time adjustment range is 0-18h. The heating rods of both are located below the dehydration chamber.

5. A nematode specimen preparation apparatus according to any one of claims 1 to 3, characterized in that, The outer insulation layer of the shell is connected.

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