Leukocyte-rich PRP extraction device

Abstract

The utility model discloses a leukocyte-rich PRP (platelet rich plasma) extraction device. The centrifugal tube comprises a centrifugal tube body and a tube cap which are buckled with each other, the centrifugal tube body sequentially comprises a first cavity, a second cavity and a third cavity from top to bottom, the first cavity, the second cavity and the third cavity are communicated in sequence, and the tube inner diameter of the second cavity is smaller than that of the third cavity; the piston is embedded in the third cavity in a manner of moving up and down; the separation device is arranged in the third cavity in a penetrating manner; the separation device moves in the length direction of the second cavity so as to communicate or close the second cavity and the third cavity; according to the scheme, the leukocyte-rich platelet-rich plasma (LR-PRP) extraction device is efficient and easy and convenient to operate, high-concentration platelets and leukocytes can be effectively separated and extracted in single operation, the operation steps are simplified, the pollution risk is reduced, the extraction efficiency and the sample activity are improved, and therefore clinical requirements are better met.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a white blood cell-rich PRP extraction device. Background Technology

[0002] Platelet-rich plasma (PRP) is widely used in modern medicine for tissue repair, regenerative medicine, and cosmetic surgery. PRP promotes tissue healing and regeneration through its high concentration of platelets and growth factors. However, different types of PRP have varying effects due to their different compositions and intended applications. Leukocyte-rich platelet-rich plasma (LR-PRP), with its high concentration of leukocytes and platelets, provides stronger immune and repair functions, exhibiting unique advantages in anti-infection, inflammation control, and tissue repair, for example:

[0003] Chronic wounds, including diabetic foot ulcers and bedsores, benefit from the high white blood cell count in LR-PRP, which helps clear necrotic tissue and fight infection.

[0004] Acute injuries: such as sports injuries, acute muscle tears and ligament injuries, LR-PRP initiates a stronger healing process by increasing the inflammatory response.

[0005] Postoperative repair: LR-PRP can promote tissue regeneration and reduce the risk of infection in postoperative wound healing and tissue repair.

[0006] Bone healing: Used for fracture healing and bone grafting, the white blood cells in LR-PRP contribute to bone regeneration and repair.

[0007] Therefore, LR-PRP has shown significant efficacy in treating chronic wounds, acute injuries, and postoperative repair.

[0008] Currently, most PRP extraction devices on the market focus on extracting ordinary PRP or low-leukocyte PRP, making it difficult to effectively extract leukocyte- and platelet-rich plasma. Existing techniques typically require multiple centrifugations and manual operations to separate leukocytes and platelets, which is not only complex and time-consuming but also prone to contamination, reducing extraction efficiency and sample purity. Furthermore, existing devices do not handle blood samples gently enough during operation, potentially leading to decreased platelet and leukocyte activity and affecting the final treatment outcome. Utility Model Content

[0009] To address the problems existing in the prior art, this invention provides a white blood cell-rich PRP extraction device, which can effectively solve the problems existing in the prior art.

[0010] The technical solution of this utility model is:

[0011] According to one aspect of the present invention, a centrifuge tube and a cap are interlocked, wherein the centrifuge tube comprises, from top to bottom, a first cavity, a second cavity, and a third cavity that are sequentially connected, and the inner diameter of the second cavity is smaller than the inner diameter of the third cavity.

[0012] It also includes a piston, which is movably fitted into the third cavity;

[0013] It also includes a separation device that passes through the third cavity, the separation device moving along the length of the second cavity to connect or close the second cavity and the third cavity.

[0014] Furthermore, the volume of the collected blood sample is defined as Lml, and the minimum volume at the top limit position of the third cavity is (0.30~0.35)*L.

[0015] Furthermore, the volume of the second cavity is (0.2~0.3)*L.

[0016] Furthermore, the inner diameter of the second cavity is 1~1.8cm.

[0017] Furthermore, it also includes a base, one end of which is fixedly connected to the piston, and the base is threadedly connected to the opening of the third cavity.

[0018] Furthermore, a connecting post is provided at one end of the base extending into the third cavity. The connecting post is provided with a limiting protrusion, and the piston is provided with a corresponding limiting groove. The limiting protrusion engages with the limiting groove.

[0019] Furthermore, the separation device includes a plug, a screw, and a knob cap, wherein the screw passes through the base and the third cavity, and the screw is threadedly connected to the base;

[0020] One end of the screw is fixed with a plug for blocking the second cavity, and the other end is fixed with a knob cap;

[0021] The plug can move along the length of the second cavity under the drive of the screw to connect or close the second cavity and the third cavity.

[0022] Furthermore, one end of the cap extends toward the inner circumferential surface of the first cavity and is provided with an annular sloping surface.

[0023] Furthermore, it also includes a bracket, which is fixedly sleeved on the outside of the cap and threadedly connected to the first cavity.

[0024] Furthermore, the cap is made of silicone.

[0025] By adopting the above technical solution, the beneficial effects of this utility model compared with the prior art are as follows:

[0026] This solution provides a highly efficient and easy-to-use leukocyte-rich platelet-rich plasma (LR-PRP) extraction device. This device can effectively separate and extract high concentrations of platelets and leukocytes in a single operation, simplifying the operation steps, reducing the risk of contamination, and improving extraction efficiency and sample activity, thereby better meeting clinical needs. LR-PRP extracted by this device will have a more significant effect in the treatment of chronic wounds, acute injuries, and postoperative repair, promoting tissue regeneration and healing, and improving patients' treatment outcomes and quality of life. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0028] Figure 1 This is a schematic diagram of the vertical cross-sectional structure of the extraction device in this utility model;

[0029] Figure 2 This is a cross-sectional view of the plug blocking the second cavity in this utility model.

[0030] Figure 3 This is a schematic diagram of the structure of the present invention, in which the syringe is inserted into the first cavity when the extraction device is rotated 180° and then turned vertically in the reverse direction;

[0031] In the diagram: centrifuge tube-1, first chamber-11, third chamber-12, second chamber-13, tube cap-2, ramp-21, bracket-3, piston-4, limiting groove-41, base-5, connecting column-51, limiting flange-52, separation device-6, plug-61, screw-62, knob cap-63, syringe-A. Detailed Implementation

[0032] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are only for illustrating the present invention and do not limit the scope of the present invention. Similarly, the following embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0033] like Figures 1 to 3 As shown, this solution provides a white blood cell-rich PRP extraction device.

[0034] Please see Figure 1 and Figure 2 The extraction device includes a centrifuge tube 1 and a cap 2 that interlock with each other. In this embodiment, it also includes a support 3, which is fixedly sleeved on the outside of the cap 2 and threadedly connected to the first cavity 11. The cap 2 is made of silicone material to facilitate the insertion of syringe A into the cap 2 for the collection of platelets, white blood cells, and plasma.

[0035] Please see Figure 1 and Figure 2 The system also includes a base 5 and a piston 4. The piston 4 is movably embedded in the third cavity 12, with one end of the piston 4 fixedly connected to the base 5. The base 5 is threadedly connected to the opening of the third cavity 12. The initial state of the base 5 is that it rotates to its limit position towards the third cavity 12. Specifically, the internal thread of the base 5 engages with the external thread of the third cavity 12. Preferably, one end of the base 5 extends into the third cavity 12 and is provided with a connecting post 51. The connecting post 51 is provided with a limiting flange 52, and the piston 4 is correspondingly provided with a limiting groove 41. The limiting flange 52 engages with the limiting groove 41.

[0036] Please see Figure 1 and Figure 2 The centrifuge tube 1 comprises, from top to bottom, three sequentially connected chambers: a first chamber 11, a second chamber 13, and a third chamber 12. The inner diameter of the second chamber 13 is smaller than that of the third chamber 12. The volume of the collected blood sample is defined as L / ml, where L can be 5-100 ml. In several embodiments, L is 10-30 ml. In this embodiment, L is approximately 20 ml. Since the red blood cell content in normal human whole blood is between 35% and 55%, to ensure that the boundary between the red blood cell layer and the white blood cell layer is located in the second chamber 13 during subsequent separation, preferably, the minimum volume at the top limit position of the third chamber 12 should be 0.30-0.35 L; more preferably, the minimum volume at the top limit position of the third chamber 12 should be 0.33-0.34 L. In this embodiment, the minimum volume at the top limit position of the third chamber 12 is approximately 6.8 ml. The maximum volume at the bottom limit position of the third chamber 12 is not limited, but preferably can be around 0.5 L. In this embodiment, the maximum volume of the third cavity 12 at its bottom limit position is about 10ml.

[0037] The volume of the second chamber 13 is preferably 0.2~0.3 L. This has the advantage that when approximately 35% of the red blood cells fill the third chamber 12 at its top limit during the first centrifugation, the remaining red blood cells will reside in the second chamber 13. This ensures that the boundary between red blood cells and white blood cells is also located within the second chamber 13, facilitating subsequent separation of the red blood cell layer and the white blood cell layer. In this embodiment, the volume of the second chamber 13 is preferably 4 ml~6 ml, and more preferably, 5 ml.

[0038] In this embodiment, furthermore, to make the boundary between red blood cells and white blood cells more distinct, the inner diameter of the second cavity 13 can be further reduced to increase its height, while keeping the volume of the second cavity 13 unchanged. However, reducing the inner diameter will also lead to incomplete stratification of red blood cells and white blood cells (as the inner diameter decreases, the centrifugal force also decreases, F = mω). 2 Where F represents centrifugal force, m represents mass, ω represents angular velocity, and r represents radius. Experiments have shown that when the inner diameter of the second chamber 13 is less than 1 cm, and the centrifugal force for the first centrifugation is 400 g (g is the acceleration due to gravity), it is difficult to effectively separate red blood cells and white blood cells. Taking a volume of 5 ml for the second chamber 13 as an example (V=π*R) 2 *h, where V represents volume, R represents radius, and h represents height), preferably, the inner diameter of the second cavity 13 is 1cm to 1.8cm, and the corresponding height of the second cavity 13 is 6.37 to 1.97cm; more preferably, the inner diameter of the second cavity 13 is 1.2cm to 1.6cm, and the corresponding height of the second cavity 13 is 4.42 to 2.49cm.

[0039] Please see Figures 1 to 3 It also includes a separation device 6 that passes through the third cavity 12. The separation device 6 moves along the length of the second cavity 13 to connect or close the second cavity 13 and the third cavity 12. Preferably, the separation device 6 includes a plug 61, a screw 62 and a knob cap 63. The screw 62 passes through the base 5 and the third cavity 12 and is threadedly connected to the base 5. The plug 61 can move along the length of the second cavity 13 under the drive of the screw 62 to connect or close the second cavity 13 and the third cavity 12. One end of the screw 62 is fixed with a plug 61 for blocking the second cavity 13, and the other end is fixed with a knob cap 63.

[0040] Please see Figure 1 and Figure 2One end of the cap 2 extends toward the inner circumferential surface of the first cavity 11 and is provided with an annular sloping surface 21. Therefore, when the extraction device is reversed and vertical, the space from the junction of the first cavity 11 and the cap 2 to the upper end surface of the first cavity 11 is wider at the top and narrower at the bottom, which facilitates observation of the collection of platelets, white blood cells and plasma.

[0041] The extraction method includes the following steps:

[0042] S1, set the extraction device vertically in the positive direction, rotate the base 5 towards the third cavity 12 to the top limit position, and then move the plug 61 away from the second cavity 13 to connect the first cavity 11, the second cavity 13 and the third cavity 12.

[0043] S2, inject the collected blood sample into centrifuge tube 1; the specific steps of injecting the collected blood sample into centrifuge tube 1 include: inserting syringe A from the top of tube cap 2 and then injecting the collected blood sample into centrifuge tube 1.

[0044] S3, Start the centrifuge, control the centrifugal force and time of the centrifuge to perform the first centrifugation, so that the blood sample is separated into red blood cell layer, white blood cell layer and plasma layer from bottom to top; Preferably, the step of controlling the centrifugal force and time of the centrifuge to perform the first centrifugation to separate the blood sample into red blood cell layer, white blood cell layer and plasma layer from bottom to top specifically includes: controlling the centrifugal force of the centrifuge to 380~420g and the centrifugation time to 3~10 minutes to perform the first centrifugation, so that the blood sample is separated into red blood cell layer, white blood cell layer and plasma layer from bottom to top.

[0045] When the boundary between the red blood cell layer and the white blood cell layer is located in the third cavity 12, a preliminary assessment of the blood problem is made, and the assessment ends. When the boundary between the red blood cell layer and the white blood cell layer is located in the second cavity 13, it indicates that the red blood cell content of the whole blood is between 35% and 55%.

[0046] When the boundary between the red blood cell layer and the white blood cell layer is located within the first cavity 11, a preliminary assessment of the blood problem is made, and the assessment ends. If the boundary between the red blood cell layer and the white blood cell layer is located within the first cavity 11, it indicates that the total red blood cell count exceeds 55%.

[0047] S4, slowly rotate the base 5 to drive the piston 4 to move downward, so that the red blood cell layer located in the second cavity 13 slowly flows into the third cavity 12, so that the boundary between the red blood cell layer and the white blood cell layer is located at the bottom of the second cavity 13.

[0048] S5, slowly rotate the knob cap 63 to move the screw 62 towards the second cavity 13, so that the plug 61 blocks the second cavity 13, thereby separating the red blood cell layer from the white blood cell layer; when the boundary between the red blood cell layer and the white blood cell layer rises significantly during the process of slowly rotating the knob cap 63 and moving the screw 62 towards the second cavity 13, slowly rotate the base 5 to move the piston 4 downward, so as to keep the boundary between the red blood cell layer and the white blood cell layer at the bottom of the second cavity 13.

[0049] S6, rotate the extraction device 180°, start the centrifuge, and control the centrifugal force and time of the centrifuge to perform a second centrifugation, so that platelets and white blood cells are concentrated at the bottom layer; preferably, the step of rotating the extraction device 180°, starting the centrifuge, and controlling the centrifugal force and time of the centrifuge to perform a second centrifugation to concentrate platelets and white blood cells at the bottom layer specifically includes:

[0050] The centrifugal force of the centrifuge is controlled at 950~1050g, and the centrifugation time is 5~15 minutes for a second centrifugation to concentrate platelets and white blood cells at the bottom layer.

[0051] S7. After inserting syringe A into the bottom of cap 2, collect the concentrated platelets, white blood cells and plasma.

[0052] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A device for extracting leukocyte-rich PRP, comprising: The centrifuge tube (1) and cap (2) that interlock with each other are characterized in that the centrifuge tube (1) comprises, from top to bottom, a first cavity (11), a second cavity (13) and a third cavity (12) that are connected in sequence, and the inner diameter of the second cavity (13) is smaller than the inner diameter of the third cavity (12). It also includes a piston (4), which is movably embedded in the third cavity (12); It also includes a separation device (6) that passes through the third cavity (12), the separation device (6) moving along the length of the second cavity (13) to connect or close the second cavity (13) and the third cavity (12).

2. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, The volume of the collected blood sample is defined as Lml, and the minimum volume of the third chamber (12) at the top limit position is (0.30~0.35)*L.

3. The leukocyte-rich PRP extraction device as described in claim 2, characterized in that, The volume of the second cavity (13) is (0.2~0.3)L.

4. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, The inner diameter of the second cavity (13) is 1 to 1.8 cm.

5. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, It also includes a base (5), one end of the piston (4) is fixedly connected to the base (5), and the base (5) is threadedly connected to the opening of the third cavity (12).

6. The leukocyte-rich PRP extraction device as described in claim 5, characterized in that, One end of the base (5) extends into the third cavity (12) and is provided with a connecting post (51). The connecting post (51) is provided with a limiting protrusion (52), and the piston (4) is provided with a limiting groove (41) accordingly. The limiting protrusion (52) engages with the limiting groove (41).

7. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, The separation device (6) includes a plug (61), a screw (62) and a knob cap (63). The screw (62) passes through the base (5) and the third cavity (12), and the screw (62) is threadedly connected to the base (5). One end of the screw (62) is fixed with a plug (61) for blocking the second cavity (13), and the other end is fixed with a knob cap (63); The plug (61) can move along the length of the second cavity (13) under the drive of the screw (62) to connect or close the second cavity (13) and the third cavity (12).

8. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, One end of the cap (2) extends toward the inner circumferential surface of the first cavity (11) and is provided with an annular sloping surface (21).

9. The leukocyte-rich PRP extraction device as described in claim 1, characterized in that, It also includes a bracket (3), which is fixedly sleeved on the outside of the cap (2) and is threadedly connected to the first cavity (11).

10. The leukocyte-rich PRP extraction device as described in claim 9, characterized in that, The cap (2) is made of silicone.

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