Transfer membrane cutting device for western blot method

Abstract

The utility model provides a transfer membrane cutting device for a western blot method. Relates to the technical field of experimental instruments, the western blot transfer membrane cutting device comprises a base, a supporting frame is fixedly installed at the top of the base, three sliding blocks are slidably installed on the inner walls of the two sides of the supporting frame, three frame blocks are fixedly installed among the six sliding blocks, a rectangular hole is formed in the supporting frame, and the rectangular hole is communicated with the rectangular hole. Two electric push rods are fixedly installed at the bottom of the supporting frame, a same driving plate is fixedly installed on output rods of the two electric push rods, two fixing blocks are fixedly installed at the bottom of the driving plate, a same double-thread screw is rotatably installed between the two fixing blocks, two movable holes are formed in the driving plate, and the two movable holes are communicated with the driving plate. And a welding block is fixedly mounted at the top of the driving plate. The cutting device has the advantages that two times of cutting can be completed at a time, the cutting size can be conveniently controlled, and the cutting efficiency can be relatively improved.

Description

Technical Field

[0001] This utility model relates to the field of experimental instrument technology, and in particular to a protein blotting transfer and cutting device. Background Technology

[0002] Western blotting is a commonly used experimental method widely applied in molecular biology, biochemistry, and immunogenetics. Its basic principle is to stain cell or biological tissue samples treated with gel electrophoresis with specific antibodies, and to obtain information on the expression of specific proteins in the analyzed cells or tissues by analyzing the location and depth of staining. PVDF membranes are used as solid-phase supports in Western blotting experiments. During the operation, after a series of treatments, the PVDF membrane needs to be transferred, that is, the PVDF membrane is cut into appropriately sized shapes and placed in the corresponding incubation box to continue the reaction.

[0003] Traditionally, cutting PVDF membranes usually requires manual operation with a small knife. During the operation, in order to ensure the uniformity of the cut size of the PVDF membrane, the operator usually needs to use a measuring ruler to compare and control the cutting position of the PVDF membrane. The whole operation process is time-consuming and labor-intensive, with low operating efficiency, and the PVDF membrane is also easily damaged during cutting.

[0004] Therefore, it is necessary to provide a new protein blotting transfer and cutting device to solve the above-mentioned technical problems. Utility Model Content

[0005] The purpose of this invention is to provide a protein blotting transfer cutting device that can complete two cuts in one step, facilitate control of the cut size, and relatively improve the cutting efficiency.

[0006] To solve the above-mentioned technical problems, the protein blotting transfer and cutting device provided by this utility model includes: a base, a support frame fixedly installed on the top of the base, three sliding blocks slidably installed on the inner walls of both sides of the support frame, three frame blocks fixedly installed between the six sliding blocks, a rectangular hole opened on the support frame, two electric push rods fixedly installed at the bottom of the support frame, a common driving plate fixedly installed on the output rods of the two electric push rods, two fixing blocks fixedly installed at the bottom of the driving plate, and a common double-headed joint rotatably installed between the two fixing blocks. The screw has two movable holes on its drive plate. A welding block is fixedly installed on the top of the drive plate. Two sliding blocks are threaded onto the double-ended screw. A U-shaped rod is fixedly installed on the top of the drive plate. Connecting blocks are fixedly installed on one side of each of the two sliding blocks and the welding block. All three connecting blocks are slidably connected to the U-shaped rod. Each of the three connecting blocks has a sliding groove. An arm is slidably installed in each of the three sliding grooves. The bottom ends of the three arms are fixedly connected to the tops of the three frame blocks respectively. A cutting blade is fixedly installed at the bottom of each of the three connecting blocks.

[0007] Preferably, the same strip plate is fixedly installed on one side of the two fixed blocks, and an indicator is fixedly installed on the top of both the welding block and the two sliding blocks.

[0008] Preferably, two upright plates are fixedly installed at the bottom of the support frame, and two U-shaped clamps are fixedly installed on one side of each of the two upright plates. The four U-shaped clamps are respectively fixedly connected to the two electric push rods.

[0009] Preferably, the inner walls on both sides of the base are provided with strip grooves, and the six sliding blocks are respectively slidably installed in the two strip grooves. Each of the six sliding blocks is embedded with a plurality of rolling beads, and the plurality of rolling beads are respectively in contact with the inner walls of the two strip grooves.

[0010] Preferably, a square block is fixedly installed at the top of each of the three arms, and two rolling balls are embedded on one side of each of the three square blocks. The six rolling balls are in contact with one side of the connecting block.

[0011] Preferably, a handle is fixedly installed at one end of the double-ended screw, and a lifting handle is fixedly installed at the top of the support frame.

[0012] Preferably, the welding block has a circular through hole, and the double-ended screw passes through the circular through hole.

[0013] Compared with related technologies, the protein blotting transfer and cutting device provided by this utility model has the following advantages:

[0014] In this invention, the distance between the three cutting blades can be adjusted as needed by manually rotating the double-headed screw. During the adjustment process, the scale lines on the bar plate can be observed with the help of three pointers, which makes it easy to determine the position of the two cutting blades in the adjusted state and can better ensure the accuracy of the adjustment. The setting of three cutting blades allows the equipment to complete at least two cuts on the PVDF film at one time, improving the cutting efficiency. The drive of two electric push rods can eliminate the manual cutting step, which is more time-saving and labor-saving, and the PVDF film is not easily damaged during the cutting process. Attached Figure Description

[0015] Figure 1 A three-dimensional structural schematic diagram of a preferred embodiment of the protein blotting transfer and cutting device provided by this utility model;

[0016] Figure 2 This is a side view of the structure of this utility model;

[0017] Figure 3 This is a frontal sectional view of the present invention.

[0018] Figure 4 This is a side sectional view of the present invention.

[0019] The following are the labels in the diagram: 1. Base; 2. Support frame; 3. Sliding block; 4. Frame block; 5. Rectangular hole; 6. Electric push rod; 7. Drive plate; 8. Fixing block; 9. Double-ended screw; 10. Movable hole; 11. Welding block; 12. Sliding block; 13. U-shaped rod; 14. Connecting block; 15. Sliding groove; 16. Arm; 17. Cutting blade; 18. Strip plate; 19. Indicator. Detailed Implementation

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Please refer to the following: Figures 1-4 ,in, Figure 1 A three-dimensional structural schematic diagram of a preferred embodiment of the protein blotting transfer and cutting device provided by this utility model; Figure 2 This is a side view of the structure of this utility model; Figure 3 This is a frontal sectional view of the present invention. Figure 4This is a side sectional view of the present invention. The protein blotting transfer and cutting device includes: a base 1, which is frame-shaped; a support frame 2, which is L-shaped, is fixedly mounted on the top of the base 1; a lifting handle is fixedly mounted on the top of the support frame 2; three sliding blocks 3 are slidably mounted on the inner walls of both sides of the support frame 2; the six sliding blocks 3 are arranged in a rectangular array; three frame blocks 4 are fixedly mounted between the six sliding blocks 3; the three frame blocks 4 are linearly distributed; a rectangular hole 5 is provided on the support frame 2 to facilitate observation of the PVDF membrane placement and to check the scale; two electric push rods 6 are fixedly mounted on the bottom of the support frame 2; the same driving plate 7 is fixedly mounted on the output rods of the two electric push rods 6; two fixing blocks 8 are fixedly mounted on the bottom of the driving plate 7; a double-ended screw 9 is rotatably mounted between the two fixing blocks 8; a handle is fixedly mounted on one end of the double-ended screw 9; two movable holes 10 are provided on the driving plate 7; and a welding block 11 is fixedly mounted on the top of the driving plate 7. A circular through hole is provided on the welding block 11, through which the double-ended screw 9 passes. The diameter of the circular through hole is larger than the diameter of the double-ended screw 9 to ensure that the welding block 11 does not contact the double-ended screw 9, thereby avoiding the double-ended screw 9 from affecting the welding block 11 when rotating. Two sliding blocks 12 are threaded on the double-ended screw 9. A U-shaped rod 13 is fixedly installed on the top of the driving plate 7. Connecting blocks 14 are fixedly installed on one side of the two sliding blocks 12 and the welding block 11. The two connecting blocks 14 are slidably installed in the two movable holes 10 respectively. All three connecting blocks 14 are slidably connected to the U-shaped rod 13. Each of the three connecting blocks 14 has a sliding groove 15. An arm 16 is slidably installed in each of the three sliding grooves 15. The three arms 16 are all L-shaped. The bottom ends of the three arms 16 are fixedly connected to the tops of the three frame blocks 4 respectively. Cutting blades 17 are fixedly installed at the bottom of each of the three connecting blocks 14. The three cutting blades 17 are respectively adapted to the three frame blocks 4.

[0022] To facilitate the determination of the positions of the two sliding blocks 12 and ensure that the spacing between the three cutting blades 17 is the same and their positions are predetermined, in this method, the same strip plate 18 is fixedly installed on one side of the two fixed blocks 8. The strip plate 18 is made of transparent acrylic material, which is convenient to view from different angles. The top of the strip plate 18 is provided with scale lines. The top of the welding block 11 and the two sliding blocks 12 are all fixedly installed with pointers 19. The position of the cutting blades 17 can be easily determined by the cooperation of the three pointers 19 and the scale lines.

[0023] To increase the stability of the installation of the two electric push rods 6, in this method, two upright plates are fixedly installed at the bottom of the support frame 2, and two U-shaped clamps are fixedly installed on one side of each of the two upright plates. The four U-shaped clamps are fixedly connected to the two electric push rods 6 respectively.

[0024] To provide sufficient space for the six sliding blocks 3 and prevent them from falling off the base 1, the base 1 has slots on both sides of its inner walls. The six sliding blocks 3 are slidably installed in the two slots. Each of the six sliding blocks 3 is inlaid with a number of rolling balls 1, which contact the inner walls of the two slots. To prevent the three arms 16 from falling off the three connecting blocks 14, square blocks are fixedly installed at the top of each of the three arms 16. Two rolling balls 2 are inlaid on one side of each of the three square blocks. The six rolling balls 2 can reduce the wear on the three arms 16. The six rolling balls 2 contact one side of each connecting block 14.

[0025] The working principle of the protein blotting transfer and cutting device provided by this invention is as follows:

[0026] When the PVDF film needs to be cut during the transfer process, first rotate the double-ended screw 9 as needed. During the rotation of the double-ended screw 9, the two sliding blocks 12 will move simultaneously on the double-ended screw 9 due to the restriction of the U-shaped rod 13. By adjusting the rotation direction of the double-ended screw 9, the movement trend of the two sliding blocks 12 can be controlled, so that the two sliding blocks 12 move closer or further away from each other. During the movement of the two sliding blocks 12, the distance between them and the welding block 11 will remain the same. During the movement of the two sliding blocks 12, the two connecting blocks 14 fixedly connected to them will be driven, and then the two connecting blocks 14 will drive the two cutting blades 17. The cutting blade 17 in the middle will always remain stationary. During the movement of the two sliding blocks 12, the two arms 16 will be driven simultaneously, and then the arms 16 will drive the two frame blocks 4 and the corresponding four sliding blocks 3 to slide on the base 1. The two pointers 19 will move simultaneously under the action of the two sliding blocks 12. By observing the scale line on the top of the strip plate 18, after determining that the two sliding blocks 12 have been adjusted to the predetermined position, the adjustment of the double-ended screw 9 can be stopped.

[0027] Next, the PVDF film to be cut is placed on top of the three frame blocks 4, and the position of the PVDF film is adjusted to ensure that the three cutting blades 17 are aligned with the appropriate cutting line. Then, the two electric push rods 6 are activated. The output rods of the two electric push rods 6 will simultaneously push outward to move the plate 7 downward. During the movement of the plate 7, the three cutting blades 17 are driven through the three connecting blocks 14. The three cutting blades 17 will gradually approach the PVDF film and gradually slide into the three frame blocks 4. After the output rods of the two electric push rods 6 extend to the predetermined position, the output rods of the two electric push rods 6 continue to extend and gradually retract inward, driving the three cutting blades 17 to gradually move upward. During the downward movement of the three cutting blades 17, the cutting of the PVDF film is completed. After the three cutting blades 17 move upward, the cut PVDF film can be removed from the base 1 and the three frame blocks 4 for the next round of cutting.

[0028] Compared with related technologies, the protein blotting transfer and cutting device provided by this utility model has the following advantages:

[0029] In this invention, the distance between the three cutting blades 17 can be adjusted as needed by manually rotating the double-headed screw 9. During the adjustment process, the scale lines on the strip plate 18 can be observed with the help of three pointers 19, which makes it easy to determine the position of the two cutting blades 17 in the adjustment state and can better ensure the accuracy of the adjustment. The setting of three cutting blades 17 allows the equipment to complete at least two cuts on the PVDF film at one time, which improves the cutting efficiency. The driving of two electric push rods 6 can eliminate the manual cutting step, which is more time-saving and labor-saving, and the PVDF film is not easily damaged during the cutting process.

[0030] It should be noted that the device structure and accompanying drawings of this utility model mainly describe the principle of this utility model. In terms of the technical aspects of this design principle, the setting of the power mechanism, power supply system and control system of the device is not fully described. However, those skilled in the art who understand the principle of the above utility model can clearly understand the specific details of its power mechanism, power supply system and control system.

[0031] The above are merely embodiments of this utility model and do not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A protein blotting transfer and cutting device, comprising: The base is characterized in that a support frame is fixedly installed on the top of the base, three sliding blocks are slidably installed on the inner walls of both sides of the support frame, three frame blocks are fixedly installed between the six sliding blocks, a rectangular hole is opened on the support frame, two electric push rods are fixedly installed on the bottom of the support frame, a common driving plate is fixedly installed on the output rods of the two electric push rods, two fixing blocks are fixedly installed on the bottom of the driving plate, a common double-ended screw is rotatably installed between the two fixing blocks, two movable holes are opened on the driving plate, a welding block is fixedly installed on the top of the driving plate, two sliding blocks are threaded on the double-ended screw, a U-shaped rod is fixedly installed on the top of the driving plate, a connecting block is fixedly installed on one side of the two sliding blocks and the welding block, the three connecting blocks are slidably connected to the U-shaped rod, a sliding groove is opened on the three connecting blocks, an arm is slidably installed in the three sliding grooves, the bottom end of the three arms is fixedly connected to the top of the three frame blocks respectively, and a cutting blade is fixedly installed on the bottom of the three connecting blocks.

2. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, The same strip plate is fixedly installed on one side of both fixed blocks, and an index is fixedly installed on the top of both the welding block and the two sliding blocks.

3. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, Two upright plates are fixedly installed at the bottom of the support frame, and two U-shaped clamps are fixedly installed on one side of each of the two upright plates. The four U-shaped clamps are respectively fixedly connected to the two electric push rods.

4. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, The base has strip grooves on both sides of its inner wall. The six sliding blocks are slidably installed in the two strip grooves respectively. Each of the six sliding blocks is inlaid with a plurality of rolling beads, and the plurality of rolling beads are in contact with the inner wall of the two strip grooves respectively.

5. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, Each of the three booms has a square block fixedly installed at its top end. Each of the three square blocks has two rolling balls embedded on one side. The six rolling balls are in contact with one side of the connecting block.

6. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, A handle is fixedly installed at one end of the double-ended screw, and a lifting handle is fixedly installed at the top of the support frame.

7. The protein blotting transfer and cutting apparatus according to claim 1, characterized in that, The welding block has a circular through hole, and the double-ended screw passes through the circular through hole.

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