Efficient fermentation system for culturing L-homoserine

Abstract

The utility model discloses an efficient fermentation system for culturing L-homoserine, and belongs to the technical field of microbial fermentation. The problems of low production efficiency and high energy consumption in the prior art are solved. According to the technical key points, the left side of a first pipeline is connected with a circulating water drainage tank, and the right side of the first pipeline is connected with a stirring tank; the left side of a second pipeline is connected with a first sewage tank, and the right side of the second pipeline is connected with the first pipeline; the left side of the seventh pipeline is connected with an ammonia water storage tank, and the right side of the seventh pipeline is connected with a stirring tank. According to the utility model, materials are supplemented into the fermentation tank according to the actual growth vigor of the fermentation tank, and strains are bred and metabolized in the fermentation tank in a fed-batch material supplementing manner, so that a required target product is finally obtained, the production efficiency is improved, the product quality is stable, the flexibility is high, the energy consumption is lower, and the environment is sustainable.

Description

Technical Field

[0001] This invention relates to the field of microbial fermentation technology, specifically to a high-efficiency fermentation system for culturing L-homoserine. Background Technology

[0002] In bio-fermentation, the fermenter plays a crucial role. It is not only the external environment for microbial growth, reproduction, and product formation, but also replaces traditional fermentation containers, providing a more rigorous sterilization environment, introducing air to create a favorable fermentation environment, and implementing measures such as stirring and shaking to promote microbial growth. Furthermore, the fermenter can automatically control temperature, pressure, and airflow, and uses various biosensors to measure the concentration of microorganisms, nutrients, and products within the fermenter, allowing for real-time computer-controlled adjustments to the fermentation process. These functions enable large-scale continuous production, maximizing the utilization of raw materials and equipment to achieve high yields and high efficiency. This product utilizes microbial fermentation technology to cultivate high-serine bacteria. Through process control, it manages temperature, pH, dissolved oxygen concentration, pressure, and ventilation. Sugars and amino acids are added to the fermenter based on the actual fermentation conditions, and the feed rate is adjusted appropriately through sampling and analysis to ensure the bacteria grow and metabolize in the most suitable environment.

[0003] This process is characterized by being non-toxic, pollution-free, and having rapid degradation. It employs bio-fermentation technology and utilizes amino acid fermentation to carry out secondary fermentation culture of homoserine-containing bacteria. The product obtained through culture is then purified through an extraction process to obtain homoserine that meets the requirements of subsequent synthesis.

[0004] In existing technologies, there are several problems in microbial fermentation, such as slow fermentation speed, unstable product quality, and microbial contamination. Due to the lack of suitable fermentation conditions, impurities inhibit microbial growth, leading to slow fermentation. Simultaneously, changes in external factors during fermentation can cause unstable product quality, such as fluctuations in pH or temperature, and insufficient mixing during the production process. Furthermore, an unclean biological environment and improper manual operation can also introduce bacteria that negatively impact the fermentation results.

[0005] Therefore, there is an urgent need to develop a high-efficiency fermentation system for cultivating L-homoserine in order to solve the problems of low production efficiency and high energy consumption. Utility Model Content

[0006] In view of the above facts, in order to solve the problems of low production efficiency and high energy consumption, this utility model designs a high-efficiency fermentation system for cultivating L-homoserine.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A high-efficiency fermentation system for cultivating L-homoserine includes a circulating water drainage tank, a stirring tank, a first wastewater tank, a circulating water return main pipe, a chilled water return main pipe, a circulating water supply main pipe, a chilled water supply main pipe, an ammonia storage tank, a first steam tank, a tail air tank, a seed transfer and distribution station, a sugar tank, a process air pre-filter, a fine filter, a sterile filter, a second steam tank, a seed tank steam filter, a waste liquid inactivation tank, a third steam tank, a fourth steam tank, a second wastewater tank, a fermentation reuse tank, a fermentation broth receiving tank, a discharge heater, a fifth steam tank, a dilute alkali tank, and a membrane hot water tank.

[0009] The first pipeline is connected to the circulating water drain tank on the left and the mixing tank on the right. The ice water drain main valve and the drain manual valve are installed on the first pipeline in sequence.

[0010] The second pipe is connected to the first sewage tank on the left and to the first pipe on the right. The right connection point is between the ice water drain main valve and the drain manual valve. An ice water drain valve is installed on the second pipe.

[0011] The third pipe is connected to the main return pipe of the circulating water on the left and to the mixing tank on the right. A return valve of the circulating water is installed on the third pipe.

[0012] The fifth pipe is connected to the main circulating water supply pipe on the left and to the first pipe on the right. The right connection point is between the drain valve and the mixing tank. The fifth pipe is installed with the circulating water inlet valve and the cooling water regulating valve in sequence.

[0013] The sixth pipe is connected to the chilled water supply main pipe on the left and to the fifth pipe on the right. The right connection point is between the circulating water inlet valve and the cooling water regulating valve. The chilled water inlet main valve and the chilled water inlet valve are installed in sequence on the sixth pipe.

[0014] The seventh pipeline is connected to the ammonia storage tank on the left and the mixing tank on the right. The ammonia remote tank valve, the ammonia automatic valve, and the ammonia near tank valve are installed sequentially on the seventh pipeline.

[0015] The eighth pipeline is connected to the first steam tank on the left and to the mixing tank on the right. The eighth pipeline is sequentially equipped with a tank discharge line sterilization valve, a tank bottom discharge valve, and a tank bottom valve.

[0016] The ninth pipe is connected to the tail gas tank on the left and the mixing tank on the right. The tail gas check valve and the tail gas automatic valve are installed in sequence on the ninth pipe.

[0017] The tenth pipeline is connected to the seed distribution station on the left and to the mixing tank on the right. An inoculation hand valve is installed on the tenth pipeline, and the first inoculation drain valve and the second inoculation drain valve are connected to the inoculation hand valve respectively.

[0018] The eleventh pipe is connected to the sugar tank on the left and the mixing tank on the right. The eleventh pipe is equipped with a manual feeding valve and an automatic feeding valve in sequence.

[0019] The twelfth pipeline connects to the main pre-filter tank of process air on the left and to the seventh pipeline on the right. The right connection point is between the ammonia water near-tank valve and the mixing tank. The twelfth pipeline is installed with the first main inlet valve, the second main inlet valve, the fine filter, the filter connecting valve, the sterile filter, the inlet regulating valve, and the inlet near-tank valve in sequence. The inlet near-tank exhaust valve is connected to the left below the inlet regulating valve, and the inlet far-tank exhaust valve is connected to the right below it.

[0020] The left side of the thirteenth pipeline connects to the second steam tank, and the right side connects to the twelfth pipeline. The right connection point is between the filter connecting valve and the sterile filter. The thirteenth pipeline is installed with the filter steam valve, the seed tank steam filter, the filter steam near the tank valve, the filter steam inlet far from the tank valve, and the filter steam inlet near the tank valve in sequence.

[0021] The fifteenth pipe connects to the thirteenth pipe on the left, with the left connection point between the second steam tank and the filter steam valve. The right side connects to the mixing tank. The fifteenth pipe is sequentially equipped with the sampling port steam main valve, the sampling port steam valve, and the sampling port tank valve.

[0022] The sixteenth pipeline connects to the fifteenth pipeline on the left, with the left connection point between the sampling port steam valve and the sampling port near the tank valve. The right side connects to the waste liquid inactivation tank. A sampling drain valve is installed on the sixteenth pipeline.

[0023] The nineteenth pipe is connected to the third steam tank on the left and to the eighteenth pipe on the right. The right connection point is between the ice water exhaust valve and the connection point of the eighteenth pipe. The first jacketed steam valve and the second jacketed steam valve are installed on the nineteenth pipe in sequence.

[0024] The 20th pipe connects to the 8th pipe on the left, with the left connection point between the bottom discharge valve and the bottom valve near the tank. The 20th pipe connects to the 4th steam tank on the right. The bottom steam valve and the bottom steam main valve are installed on the 20th pipe in sequence.

[0025] The left side of the 21st pipe connects to the 20th pipe, with the left connection point between the left connection point of the 20th pipe and the steam valve at the bottom of the tank. The right side connects to the second sewage tank. The bottom drain valve and the main drain valve at the bottom of the tank are installed sequentially on the 21st pipe.

[0026] The 22nd pipe connects to the 21st pipe on the left, with the left connection point between the tank bottom drain valve and the tank bottom drain main valve. The 22nd pipe connects to the fermentation reuse tank on the right, and a tank bottom to reuse tank valve is installed on the 22nd pipe.

[0027] The 23rd pipeline connects to the 8th pipeline on the left, with the left connection point between the sterilization valve and the bottom discharge valve of the tank discharge pipeline. The 23rd pipeline connects to the fermentation liquid receiving tank on the right. The discharge heater and fermentation discharge valve are installed on the 23rd pipeline in sequence.

[0028] The left side of the 24th pipeline is connected to the discharge heater, and the right side is connected to the fifth steam tank. The discharge heater regulating valve and the discharge heater main steam valve are installed sequentially on the 24th pipeline.

[0029] The 25th pipeline is connected to the discharge heater on the left and the dilute alkali tank on the right. The 25th pipeline is sequentially equipped with the alkali inlet valves of the first fermentation tank and the second fermentation tank.

[0030] The 26th pipe is connected to the discharge heater on the left and the membrane hot water tank on the right. The discharge heater water supply valve group is installed on the 26th pipe.

[0031] Furthermore: the fourth pipe is connected to the chilled water return main pipe on the left and to the third pipe on the right. The right connection point is between the circulating water return valve and the mixing tank. The chilled water return main valve and the chilled water return valve are installed in sequence on the fourth pipe.

[0032] Furthermore: the fourteenth pipe connects to the thirteenth pipe on the left, with the left connection point between the filter inlet steam remote valve and the filter inlet steam near valve, and the right side connects to the filter steam drain.

[0033] Furthermore: the 17th pipe is connected to the 13th pipe on the left, with the left connection point between the left connection point of the 15th pipe and the filter steam valve; the 17th pipe is connected to the 12th pipe on the right, with the right connection point between the air inlet regulating valve and the air inlet tank valve; the 17th pipe is equipped with the main steam remote tank valve and the main steam valve in sequence.

[0034] Furthermore: the eighteenth pipe connects to the third pipe, with the connection point between the circulating water return valve and the mixing tank, and an ice water vent valve is installed on the eighteenth pipe.

[0035] The beneficial effects of this utility model are as follows:

[0036] 1. This utility model has high production efficiency and can be customized. Equipped with an automatic control system, it can adjust fermentation conditions, such as temperature, pH value, and dissolved oxygen, according to different production needs to adapt to the growth and metabolic needs of different types of microorganisms and ensure optimal growth and metabolic conditions for microorganisms.

[0037] 2. The product quality of this utility model is more stable. The sealed structure of the fermentation tank effectively prevents external pollution, avoids material leakage, and ensures the sterility of the fermentation process. Furthermore, the control system can ensure a stable supply of various nutrients and oxygen during the fermentation process, thereby improving the quality and consistency of the product. The waste gas generated can be treated by a special purification system to ensure environmental sustainability.

[0038] 3. This utility model has low energy consumption, adopts a high-efficiency agitator and ventilation system to reduce energy consumption, and some fermenters are also equipped with an energy recovery system to further reduce energy costs.

[0039] 4. This utility model is highly flexible. The fermenter can process not only liquid raw materials but also solid raw materials, and is suitable for various types of microbial fermentation processes, giving the fermenter wide applicability and flexibility. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the structure of this utility model;

[0041] In the diagram: 1-Circulating water drain tank, 2-Mixing tank, 3-Ice water drain main valve, 4-Drain manual valve, 5-First sewage tank, 6-Ice water drain valve, 7-Circulating water return main pipe, 8-Circulating water return valve, 9-Iced water return main pipe, 10-Iced water return main valve, 11-Iced water return valve, 12-Circulating water supply main pipe, 13-Circulating water inlet valve, 14-Cooling water regulating valve, 15-Iced water supply main pipe, 16-Iced water inlet main valve, 17-Iced water inlet valve, 18-Ammonia storage tank, 19-Ammonia remote tank valve, 20-Automatic ammonia inlet valve, 21-Ammonia near-field valve. Tank valves, 22-First steam tank, 23-Discharge pipeline sterilization valve, 24-Tank bottom discharge valve, 25-Tank bottom valve, 26-Tail air tank, 27-Tail gas check valve, 28-Tail gas automatic valve, 29-Seed transfer distribution station, 30-Inoculation manual valve, 31-First inoculation drain valve, 32-Second inoculation drain valve, 33-Sugar tank, 34-Feeding manual valve, 35-Feeding automatic valve, 36-Process air main pre-filter tank, 37-First main air inlet valve, 38-Second main air inlet valve, 39-Fine filter, 40-Filter connection valve, 41-Sterile filter, 42-Inlet air regulator 43-Air inlet valve, 44-Second steam tank, 45-Filter steam valve, 46-Seed tank steam filter, 47-Filter steam valve, 48-Filter steam inlet valve, 49-Filter steam inlet valve, 50-Filter steam exhaust valve, 51-Sampling port main steam valve, 52-Sampling port steam valve, 53-Sampling port valve, 54-Waste liquid inactivation tank, 55-Sampling drain valve, 56-Main steam valve, 57-Main steam valve, 58-Ice water exhaust valve, 59-Third steam tank, 60-First jacket steam valve, 61-Second jacket Steam valve, 62-Fourth steam tank, 63-Bottom steam valve, 64-Bottom steam main valve, 65-Second wastewater tank, 66-Bottom drain valve, 67-Bottom drain main valve, 68-Fermentation reuse tank, 69-Bottom return-to-reuse tank valve, 70-Fermentation broth receiving tank, 71-Discharge heater, 72-Fermentation discharge valve, 73-Fifth steam tank, 74-Discharge heater regulating valve, 75-Discharge heater steam main valve, 76-Dilute alkali tank, 77-First fermentation tank alkali inlet valve, 78-Second fermentation tank alkali inlet valve, 79-Membrane hot water tank, 80-Discharge heater water supply valve assembly. Detailed Implementation

[0042] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0043] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0044] In this application, the terms "upper," "lower," "inner," "middle," "outer," "front," and "rear," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0045] Furthermore, in addition to indicating location or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0046] Furthermore, the terms "set up," "connect," and "fix" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0047] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0048] The preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0049] Example 1: This example describes a high-efficiency fermentation system for cultivating L-homoserine, comprising a circulating water drainage tank 1, a stirring tank 2, a first wastewater tank 5, a circulating water return main pipe 7, a chilled water return main pipe 9, a circulating water supply main pipe 12, a chilled water supply main pipe 15, an ammonia storage tank 18, a first steam tank 22, a tail air tank 26, a seed transfer and distribution station 29, a sugar tank 33, a process air pre-filter tank 36, a fine filter 39, a sterile filter 41, a second steam tank 44, a seed tank steam filter 46, a waste liquid inactivation tank 54, a third steam tank 59, a fourth steam tank 62, a second wastewater tank 65, a fermentation reuse tank 68, a fermentation broth receiving tank 70, a discharge heater 71, a fifth steam tank 73, a dilute alkali tank 76, and a membrane hot water tank 79.

[0050] The first pipeline is connected to the circulating water drain tank 1 on the left and the mixing tank 2 on the right. The ice water drain main valve 3 and the drain manual valve 4 are installed in sequence on the first pipeline.

[0051] The second pipe is connected to the first sewage tank 5 on the left and to the first pipe on the right. The right connection point is between the ice water drain main valve 3 and the drain manual valve 4. An ice water drain valve 6 is installed on the second pipe.

[0052] The third pipe is connected to the circulating water return main pipe 7 on the left and to the mixing tank 2 on the right. A circulating water return valve 8 is installed on the third pipe.

[0053] The fifth pipeline is connected to the circulating water supply main pipe 12 on the left and to the first pipeline on the right. The right connection point is between the drain valve 4 and the mixing tank 2. The fifth pipeline is installed with the circulating water inlet valve 13 and the cooling water regulating valve 14 in sequence.

[0054] The sixth pipe is connected to the chilled water supply main pipe 15 on the left and the fifth pipe on the right. The right connection point is between the circulating water inlet valve 13 and the cooling water regulating valve 14. The sixth pipe is installed with the chilled water inlet main valve 16 and the chilled water inlet valve 17 in sequence.

[0055] The seventh pipeline is connected to the ammonia storage tank 18 on the left and the mixing tank 2 on the right. The seventh pipeline is sequentially equipped with an ammonia remote tank valve 19, an ammonia automatic valve 20, and an ammonia near tank valve 21.

[0056] The eighth pipeline is connected to the first steam tank 22 on the left and to the mixing tank 2 on the right. The eighth pipeline is sequentially equipped with a tank discharge line sterilization valve 23, a tank bottom discharge valve 24, and a tank bottom valve 25.

[0057] The ninth pipe is connected to the tail gas tank 26 on the left and the mixing tank 2 on the right. The tail gas check valve 27 and the tail gas automatic valve 28 are installed in sequence on the ninth pipe.

[0058] The tenth pipeline is connected to the seed distribution station 29 on the left and to the mixing tank 2 on the right. An inoculation hand valve 30 is installed on the tenth pipeline. The first inoculation drain valve 31 and the second inoculation drain valve 32 are connected to the inoculation hand valve 30 respectively.

[0059] The eleventh pipe is connected to sugar tank 33 on the left and to mixing tank 2 on the right. The eleventh pipe is equipped with a manual feeding valve 34 and an automatic feeding valve 35 in sequence.

[0060] The twelfth pipeline is connected to the process air pre-filter tank 36 on the left and to the seventh pipeline on the right. The right connection point is between the ammonia water near-tank valve 21 and the mixing tank 2. The twelfth pipeline is installed with the first air inlet main valve 37, the second air inlet main valve 38, the fine filter 39, the filter connecting valve 40, the sterile filter 41, the air inlet regulating valve 42, and the air inlet near-tank valve 43 in sequence. The air inlet near-tank exhaust valve is connected to the left below the air inlet regulating valve 42, and the air inlet far-tank exhaust valve is connected to the right below it.

[0061] The thirteenth pipeline connects to the second steam tank 44 on the left and to the twelfth pipeline on the right. The right connection point is between the filter connecting valve 40 and the sterile filter 41. The thirteenth pipeline is installed with the filter steam valve 45, the seed tank steam filter 46, the filter steam near the tank valve 47, the filter steam inlet far from the tank valve 48, and the filter steam inlet near the tank valve 49 in sequence.

[0062] The fifteenth pipeline is connected to the thirteenth pipeline on the left side, with the left connection point between the second steam tank 44 and the filter steam valve 45. The right side is connected to the mixing tank 2. The fifteenth pipeline is sequentially equipped with the sampling port steam main valve 51, the sampling port steam valve 52, and the sampling port tank valve 53.

[0063] The sixteenth pipeline is connected to the fifteenth pipeline on the left side. The left connection point is between the sampling port steam valve 52 and the sampling port tank valve 53. The right side is connected to the waste liquid inactivation tank 54. A sampling drain valve 55 is installed on the sixteenth pipeline.

[0064] The nineteenth pipe is connected to the third steam tank 59 on the left and to the eighteenth pipe on the right. The right connection point is between the ice water exhaust valve 58 and the connection point of the eighteenth pipe. The first jacketed steam valve 60 and the second jacketed steam valve 61 are installed on the nineteenth pipe in sequence.

[0065] The 20th pipeline connects to the 8th pipeline on the left, with the left connection point between the bottom discharge valve 24 and the bottom valve 25. The 20th pipeline connects to the fourth steam tank 62 on the right. The bottom steam valve 63 and the bottom steam main valve 64 are installed on the 20th pipeline in sequence.

[0066] The 21st pipe is connected to the 20th pipe on the left side, with the left connection point between the left connection point of the 20th pipe and the steam valve 63 at the bottom of the tank. The 21st pipe is connected to the second sewage tank 65 on the right side. The bottom drain valve 66 and the main drain valve 67 at the bottom of the tank are installed in sequence on the 21st pipe.

[0067] The 22nd pipeline connects to the 21st pipeline on the left, with the left connection point between the tank bottom drain valve 66 and the tank bottom drain main valve 67. The 22nd pipeline connects to the fermentation reuse tank 68 on the right, and the tank bottom to reuse tank valve 69 is installed on the 22nd pipeline.

[0068] The 23rd pipeline connects to the 8th pipeline on the left, with the left connection point between the tank discharge pipeline disinfection valve 23 and the tank bottom discharge valve 24. The 23rd pipeline connects to the fermentation liquid receiving tank 70 on the right. The 23rd pipeline is sequentially equipped with the discharge heater 71 and the fermentation discharge valve 72.

[0069] The 24th pipeline is connected to the discharge heater 71 on the left and the fifth steam tank 73 on the right. The discharge heater regulating valve 74 and the discharge heater steam main valve 75 are installed sequentially on the 24th pipeline.

[0070] The 25th pipeline is connected to the discharge heater 71 on the left and the dilute alkali tank 76 on the right. The 25th pipeline is sequentially equipped with the first fermentation tank alkali inlet valve 77 and the second fermentation tank alkali inlet valve 78.

[0071] The 26th pipeline is connected to a discharge heater 71 on the left and a membrane hot water tank 79 on the right. A discharge heater water supply valve group 80 is installed on the 26th pipeline.

[0072] More specifically: the fourth pipe is connected to the chilled water return main pipe 9 on the left and to the third pipe on the right. The right connection point is between the circulating water return valve 8 and the mixing tank 2. The fourth pipe is equipped with the chilled water return main valve 10 and the chilled water return valve 11 in sequence.

[0073] More specifically: the fourteenth pipe connects to the thirteenth pipe on the left, with the left connection point between the filter steam inlet remote valve 48 and the filter steam inlet near valve 49, and the filter steam drain 50 on the right.

[0074] More specifically: the 17th pipe is connected to the 13th pipe on the left, with the left connection point between the left connection point of the 15th pipe and the filter steam valve 45. The 17th pipe is connected to the 12th pipe on the right, with the right connection point between the air inlet regulating valve 42 and the air inlet valve 43. The 17th pipe is equipped with the main steam remote valve 56 and the main steam valve 57 in sequence.

[0075] More specifically: the eighteenth pipe connects to the third pipe, with the connection point between the circulating water return valve 8 and the mixing tank 2, and an ice water vent valve 58 is installed on the eighteenth pipe.

[0076] Example 2: A high-efficiency fermentation system for culturing L-homoserine, the operation procedure of the fermenter is as follows:

[0077] Step 1: Sterilize the fermenter before cultivation to maintain a sterile state;

[0078] Step 2, Emptying;

[0079] Step 3: Add water to make up the volume, and add the culture medium into the tank;

[0080] Step 4: Disinfect and wait for vaccination;

[0081] Step 5: Transfer the inoculum to the fermenter and start fermentation. After the initial sugar in the culture medium in the tank is exhausted, take a sample for testing. Replenish the culture medium according to the results. Take a sample for analysis every 3 hours until fermentation is completed.

[0082] More specifically: the specific implementation process of step 1 is as follows:

[0083] Before sterilization, check that the tank is empty, close the inlet, and notify the instrumentation personnel to calibrate and install the pH and dissolved oxygen electrodes. Then, pressurize the tank to 1.8 kPa with air and test for leaks using foam water. Leak testing should primarily cover the manhole cover, mechanical seal, sight glass, valve connections and valves themselves, and tank welds. Check that the cooling water has been drained, the cooling water inlet and outlet valves are closed, the inlet and outlet valves are fully open, the air inlet regulating valve is closed, the exhaust gas automatic valve is fully open, and the inoculation tank is secure. Check if the valves are closed, if the sampling port valve is fully open, open the air inlet valve to 1 / 4, open the inoculation valve to 1 / 4, and open the sampling discharge valve to 1 / 4. Pour ammonia into the tank to ensure the pipeline is full of ammonia. Check if the inoculation valve is closed. Open the inoculation discharge valve to 1 / 4, close the automatic sugar replenishment valve, open the discharge valve on the tank side to 1 / 4, fully open the bottom discharge valve, close the discharge valve of the distant tank, open the return tank valve, and open the bottom discharge valve to 1 / 4.

[0084] More specifically: the specific implementation process of step 2 is as follows:

[0085] Fully open both manual valves of the bottom-inlet steam tank. Slowly open the manual diaphragm valve of the tank steam valve to 3-4 turns to raise the temperature. When the tank temperature reaches 90 degrees Celsius, the main control room will close the exhaust gas automatic valve slightly. Check whether the opening and closing of each exhaust valve is normal and whether there is steam flow. Start the air sterilization timer when the air sterilization temperature reaches 125 degrees Celsius and the pressure reaches 1.2 kPa or above. The time is 30 minutes. During the timer, the on-site and main control room operators should pay attention to the air sterilization temperature and pressure. It should not be too high (too high will damage the dissolved oxygen and pH electrodes) nor too low. At the same time, the on-site operators should check whether the exhaust of each drain valve is normal and the condensate status in the tank (if there is too much condensate in the tank, open the drain valve of the inoculation tank and then close it several times to drain the condensate). After the timer ends, depressurize and close the steam near the tank valve, sampling port valve, discharge valve, inoculation near the tank drain valve, sugar replenishment near the tank drain valve, tank bottom discharge valve, and tank bottom drain valve. After checking that the tank pressure has dropped to zero, open the manhole cover. The air sterilization is over.

[0086] More specifically: the specific implementation process of step 3 is as follows:

[0087] After the air sterilization process is complete, add water to the tank to bring the volume to 10m³. 3 Stop adding water when the volume reaches 20m³, add culture medium to the tank, and continue adding water until the tank volume reaches 20m³. 3 When adding the culture medium, contact the main control room to start the agitator. The agitator speed is 150 r / min. The purpose of starting the agitator is to completely dissolve the material in the tank and ensure that the material in the tank is heated evenly during the actual sterilization process. Close the manhole cover, check that the exhaust gas automatic valve is fully open, the air inlet regulating valve is closed, the sugar replenishment two-position four-way valve is closed, and the small exhaust valve on the side of the tank is opened 1 / 4 to prepare for actual sterilization.

[0088] More specifically: the specific implementation process of step 4 is as follows:

[0089] Open the steam hand valve of the ice water jacket 2-3 turns to heat the material inside the tank using the jacket. When the temperature inside the tank reaches 90 degrees Celsius, open the steam hand valve near the tank 2 turns. Heating with the jacket first is to avoid increasing the tank volume if steam is directly introduced into the tank due to the low internal temperature. Keep the air inlet and outlet slightly open, just enough for airflow. Close the hand valve for transferring the seed to the distribution plate. Open the drain valve near the tank 1 / 4 of the way. Open the steam valve at the sampling port. Close the discharge valve at the sampling port. Fully open the sampling port valve to introduce steam into the tank. Open the discharge valve near the tank bottom 1 turn and close the valve at the bottom of the tank. Open all drain valves at the bottom of the tank and the bottom steam valve to begin heating. Manual valves are controlled by on-site operators, while automatic valves are controlled by personnel in the main control room. When the temperature reaches 115 degrees Celsius, close the jacket steam manual valve and open the jacket exhaust valve. When the temperature reaches 121 degrees Celsius and the pressure reaches 1.0 kPa, start the sterilization timer for 20 minutes. Alternate between bottom steam and bottom-inlet steam. During the timer, ensure the sterilization temperature inside the tank does not fall below 121 degrees Celsius, while also avoiding excessive temperature rise that could lead to nutrient loss in the culture medium. Ensure all exhaust valves are functioning normally during the timer, and every five minutes, fully open each exhaust valve and then close it back to its original position. Then, maintain pressure and ventilate. When the temperature drops below 35 degrees Celsius, sample the sugar content and pH value of the culture medium inside the tank (sugar content is generally around 15 g / L, and pH value is generally around 4). Introduce ammonia into the tank to adjust the pH to 7, and wait for inoculation.

[0090] More specifically: the specific implementation process of air exchange and pressure maintenance in step 4 is as follows:

[0091] During ventilation, close both valves of the bottom steam inlet far from the tank, fully open the steam diaphragm valve near the tank, close the small exhaust valve of the air inlet near the tank, close the drain valve of the seed transfer near the tank, close the drain valve of the two-position four-way valve of the material replenishment near the tank, close the valve of the sampling port near the tank, slightly open the discharge valve of the sampling port to exhaust air, do not close the sampling steam valve, close the bottom discharge manual valve, close the bottom drain valve, close the bottom steam valve, and after the valve processing is completed, open the automatic air inlet valve to ventilate the tank, controlling the ventilation volume at 20m³.3 The pressure inside the tank is maintained at 0.4 kPa per hour. Then, the circulating water inlet and outlet valves are opened, the cooling water vent valve is closed, and the automatic water inlet valve is opened to cool the water. Pay attention to the pressure inside the tank to avoid pressure loss after the actual cooling process. Then check the valve status and end the actual cooling process after confirming that everything is correct.

[0092] More specifically: the specific implementation process of step 5 is as follows:

[0093] The inoculum was transferred to the fermenter, which was ready for inoculation, using a transfer tray. During the transfer, the pressure in the seed tank was appropriately increased to 0.12 MPa, while the pressure in the fermenter remained constant. Stirring in the fermenter was stopped during inoculation, and restarted after inoculation at an initial speed of 150 rpm. The fermenter was controlled online based on feedback from the control room, maintaining the pH at 7 and the temperature at 30 degrees Celsius. Dissolved oxygen levels in the fermenter were initially high after inoculation, but naturally decreased with time. When dissolved oxygen levels fell below 25%, the stirring speed was adjusted, while the ventilation volume and internal pressure remained constant (airflow 20 m³ / min). 3 / hour, pressure 0.04MPa), after the initial sugar in the culture medium in the tank is exhausted, take a sample to test the residual sugar and OD value of the fermentation broth, replenish the material according to the results, and take a sample for analysis every 3 hours until the fermentation is over.

[0094] Homoserine fermentation utilizes the principle of microbial fermentation, controlling the temperature at 30 degrees Celsius, the pH at 7, and the pressure in the seed tank and fermenter at 0.4 kg / cm² (the breeding room is under normal pressure and sterile conditions during the breeding process). The dissolved oxygen in the fermenter (seed tank) is controlled between 25-35%. After the inoculum is introduced into the fermenter, feed is added to the fermenter according to the actual growth of the inoculum. Through a fed-batch feeding method, the inoculum multiplies and metabolizes in the fermenter, ultimately yielding the desired product.

[0095] More specifically: through process and formula improvements, the acid production of high serine has increased from about 60g / L to over 80g / L, the sugar-acid conversion rate has increased from about 35% to over 40%, and the production cost per ton has decreased from over 35,000 yuan to about 25,000 yuan.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it; although the utility model has been described in detail with reference to the foregoing embodiments, modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; as long as there is no structural conflict, the features in the specific embodiments disclosed in this application can be combined with each other in any way, and will not cause the substance of the corresponding technical solutions to deviate from the scope of the technical solutions of this utility model.

[0097] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A high-efficiency fermentation system for culturing L-homoserine, characterized in that, The system includes a circulating water drainage tank (1), a mixing tank (2), a first sewage tank (5), a circulating water return main pipe (7), a chilled water return main pipe (9), a circulating water supply main pipe (12), a chilled water supply main pipe (15), an ammonia storage tank (18), a first steam tank (22), a tail air tank (26), a seed distribution station (29), a sugar tank (33), a process air pre-filter tank (36), a fine filter (39), a sterile filter (41), a second steam tank (44), a seed tank steam filter (46), a waste liquid inactivation tank (54), a third steam tank (59), a fourth steam tank (62), a second sewage tank (65), a fermentation reuse tank (68), a fermentation liquid receiving tank (70), a discharge heater (71), a fifth steam tank (73), a dilute alkali tank (76), and a membrane hot water tank (79). The first pipeline is connected to the circulating water drain tank (1) on the left and the mixing tank (2) on the right. The ice water drain main valve (3) and the drain manual valve (4) are installed in sequence on the first pipeline. The second pipe is connected to the first sewage tank (5) on the left and to the first pipe on the right. The right connection point is between the ice water drain main valve (3) and the drain manual valve (4). An ice water drain valve (6) is installed on the second pipe. The third pipe is connected to the circulating water return main pipe (7) on the left and to the mixing tank (2) on the right. A circulating water return valve (8) is installed on the third pipe. The fifth pipeline is connected to the circulating water supply main pipe (12) on the left and to the first pipeline on the right. The right connection point is between the drain valve (4) and the mixing tank (2). The fifth pipeline is installed with the circulating water inlet valve (13) and the cooling water regulating valve (14) in sequence. The sixth pipe is connected to the chilled water supply main pipe (15) on the left and to the fifth pipe on the right. The right connection point is between the circulating water inlet valve (13) and the cooling water regulating valve (14). The sixth pipe is installed with the chilled water inlet main valve (16) and the chilled water inlet valve (17) in sequence. The seventh pipeline is connected to the ammonia storage tank (18) on the left and the mixing tank (2) on the right. The seventh pipeline is equipped with the ammonia remote tank valve (19), the ammonia automatic valve (20), and the ammonia near tank valve (21) in sequence. The eighth pipeline is connected to the first steam tank (22) on the left and to the mixing tank (2) on the right. The eighth pipeline is equipped with the tank discharge line sterilization valve (23), the tank bottom discharge valve (24), and the tank bottom valve (25) in sequence. The ninth pipe is connected to the tail gas tank (26) on the left and the mixing tank (2) on the right. The tail gas check valve (27) and the tail gas automatic valve (28) are installed in sequence on the ninth pipe. The tenth pipeline is connected to the seed distribution station (29) on the left and to the mixing tank (2) on the right. An inoculation hand valve (30) is installed on the tenth pipeline. The first inoculation drain valve (31) and the second inoculation drain valve (32) are connected to the inoculation hand valve (30) respectively. The eleventh pipe is connected to the sugar tank (33) on the left and the mixing tank (2) on the right. The eleventh pipe is equipped with a manual feeding valve (34) and an automatic feeding valve (35) in sequence. The twelfth pipeline is connected to the process air pre-filter tank (36) on the left and to the seventh pipeline on the right. The right connection point is between the ammonia water near tank valve (21) and the mixing tank (2). The twelfth pipeline is installed with the first air inlet main valve (37), the second air inlet main valve (38), the fine filter (39), the filter connecting valve (40), the sterile filter (41), the air inlet regulating valve (42), and the air inlet near tank valve (43) in sequence. The air inlet regulating valve (42) is connected to the air inlet near tank exhaust valve on the left below and the air inlet far tank exhaust valve on the right below. The thirteenth pipeline is connected to the second steam tank (44) on the left and to the twelfth pipeline on the right. The right connection point is between the filter connecting valve (40) and the sterile filter (41). The thirteenth pipeline is installed with the filter steam valve (45), the seed tank steam filter (46), the filter steam near the tank valve (47), the filter steam far from the tank valve (48), and the filter steam near the tank valve (49) in sequence. The fifteenth pipeline is connected to the thirteenth pipeline on the left side. The left connection point is between the second steam tank (44) and the filter steam valve (45). The right side is connected to the mixing tank (2). The fifteenth pipeline is installed with the sampling port steam main valve (51), the sampling port steam valve (52), and the sampling port tank valve (53) in sequence. The sixteenth pipeline is connected to the fifteenth pipeline on the left side. The left connection point is between the sampling port steam valve (52) and the sampling port tank valve (53). The right side is connected to the waste liquid inactivation tank (54). A sampling drain valve (55) is installed on the sixteenth pipeline. The nineteenth pipe is connected to the third steam tank (59) on the left and to the eighteenth pipe on the right. The right connection point is between the ice water exhaust valve (58) and the connection point of the eighteenth pipe. The nineteenth pipe is equipped with the first jacketed steam valve (60) and the second jacketed steam valve (61) in sequence. The 20th pipeline is connected to the 8th pipeline on the left side. The left connection point is between the bottom discharge valve (24) and the bottom valve (25). The 20th pipeline is connected to the 4th steam tank (62) on the right side. The bottom steam valve (63) and the bottom steam main valve (64) are installed on the 20th pipeline in sequence. The 21st pipeline is connected to the 20th pipeline on the left side. The left connection point is between the left connection point of the 20th pipeline and the bottom steam valve (63). The 21st pipeline is connected to the second sewage tank (65) on the right side. The bottom drain valve (66) and the bottom drain main valve (67) are installed on the 21st pipeline in sequence. The 22nd pipeline is connected to the 21st pipeline on the left side. The left connection point is between the bottom drain valve (66) and the bottom drain main valve (67). The right side is connected to the fermentation reuse tank (68). The 22nd pipeline is equipped with a bottom-to-reuse tank valve (69). The 23rd pipeline is connected to the 8th pipeline on the left side. The left connection point is between the sterilization valve (23) of the tank discharge pipeline and the bottom discharge valve (24) of the tank. The fermentation liquid receiving tank (70) is connected to the 23rd pipeline in sequence. The discharge heater (71) and the fermentation discharge valve (72) are installed on the 23rd pipeline in sequence. The 24th pipeline is connected to the discharge heater (71) on the left and the fifth steam tank (73) on the right. The discharge heater regulating valve (74) and the discharge heater steam main valve (75) are installed in sequence on the 24th pipeline. The 25th pipeline is connected to the discharge heater (71) on the left and the dilute alkali tank (76) on the right. The 25th pipeline is installed with the first fermentation tank alkali inlet valve (77) and the second fermentation tank alkali inlet valve (78) in sequence. The 26th pipe is connected to a discharge heater (71) on the left and a membrane hot water tank (79) on the right. A discharge heater water supply valve assembly (80) is installed on the 26th pipe.

2. The high-efficiency fermentation system for culturing L-homoserine according to claim 1, characterized in that: The fourth pipe is connected to the chilled water return main pipe (9) on the left and to the third pipe on the right. The right connection point is between the circulating water return valve (8) and the mixing tank (2). The chilled water return main valve (10) and the chilled water return valve (11) are installed in sequence on the fourth pipe.

3. The high-efficiency fermentation system for culturing L-homoserine according to claim 1, characterized in that: The fourteenth pipe is connected to the thirteenth pipe on the left. The left connection point is between the filter inlet steam remote valve (48) and the filter inlet steam near valve (49). The right side is connected to the filter steam drain (50).

4. The high-efficiency fermentation system for culturing L-homoserine according to claim 1, characterized in that: The 17th pipe is connected to the 13th pipe on the left side, with the left connection point between the left connection point of the 15th pipe and the filter steam valve (45). The 17th pipe is connected to the 12th pipe on the right side, with the right connection point between the air inlet regulating valve (42) and the air inlet tank valve (43). The 17th pipe is equipped with the main steam remote tank valve (56) and the main steam valve (57) in sequence.

5. The high-efficiency fermentation system for culturing L-homoserine according to claim 1, characterized in that: The eighteenth pipe connects to the third pipe, with the connection point between the circulating water return valve (8) and the mixing tank (2). An ice water vent valve (58) is installed on the eighteenth pipe.

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