Single-channel fluorescence immunoassay analyzer

Abstract

The utility model provides a single-channel fluorescence immunoassay analyzer which comprises a detection device, a shell, a switch button and a display screen, the shell is provided with a containing cavity, the detection device is installed in the containing cavity of the shell, and the display screen is installed above the shell; the detection device is provided with a light box detection assembly, and the light box detection assembly comprises a light box left shell, a light box right shell, an LED lamp panel, a first plano-convex lens, a dichroscope, a second plano-convex lens, a third plano-convex lens, an optical filter and a receiving plate assembly. According to the single-channel fluorescence immunoassay analyzer disclosed by the utility model, the problems that the effective signal value acquisition of the existing equipment is weakened, the excitation efficiency and the receiving efficiency are greatly lost, and the optimal signal-to-noise ratio during acquisition cannot be realized are solved, the sensitivity of the fluorescence immunoassay analyzer is improved, the signal value of fluorescence acquisition is increased, the signal-to-noise ratio is enhanced, and the detection accuracy is improved. And a relatively accurate detection result can be given when a low-concentration sample is detected.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence detection, and in particular to a single-channel fluorescence immunoassay analyzer. Background Technology

[0002] Fluorescence immunochromatography (FIC) is a novel membrane detection technology based on antigen-antibody specific immune reactions. This method is characterized by its specificity, simplicity, and speed, and is widely used in important fields such as clinical diagnosis, environmental monitoring, and food safety. Currently, there are many types of FIC analyzers on the market, which can be categorized into single-channel, handheld, multi-channel, and fully automated FIC analyzers. Different types of instruments can be selected depending on the application scenario, but the detection principle remains the same: they all use emitted light to excite fluorescent substances and collect the fluorescence signal emitted by the substances. The magnitude of the fluorescence signal reflects the concentration of the detected substance in the sample, providing diagnostic information for doctors in IVD clinical diagnosis.

[0003] In existing detection optical paths, the excitation spot is generally circular and does not completely cover the detection lines on the reagent card. Therefore, the detected signal value is only a portion of the sample's signal value. If the excitation spot completely covers the detection lines, although the signal value increases, the background noise also increases accordingly, and the detection sensitivity cannot be significantly improved. Even if some methods use rectangular excitation spots to reduce background noise, these rectangular spots are formed through physical obstruction. When acquiring signal values, the acquisition aperture is a rectangular strip, which greatly reduces the signal entry point and only detects a small portion of the signal value, weakening the acquisition of effective signal values. The drawback of the above solutions is that the excitation and reception efficiency are greatly reduced, and the optimal signal-to-noise ratio cannot be achieved during acquisition. Utility Model Content

[0004] The main objective of this invention is to provide a single-channel fluorescence immunoassay analyzer that overcomes the above-mentioned technical problems.

[0005] To achieve the above objectives, the present invention proposes the following technical solution:

[0006] A single-channel fluorescence immunoassay analyzer, characterized in that it comprises: a detection device, a housing, a switch button, and a display screen, wherein the housing has a receiving cavity, the detection device is installed in the receiving cavity of the housing, and the display screen is installed on the top of the housing;

[0007] The detection device is equipped with a light box detection assembly, which includes a left light box housing and a right light box housing. The left and right light box housings cooperate to form an accommodating space. An LED light board is installed inside the accommodating space to provide excitation light for detection. A first plano-convex lens and a dichroic mirror are sequentially installed on one side of the lower end of the LED light board. A second plano-convex lens is installed below the dichroic mirror. A third plano-convex lens is installed above the dichroic mirror. A filter is installed above the third plano-convex lens. A receiving plate assembly is installed above the filter.

[0008] Furthermore, the housing includes an outer shell and an inner shell, the inner shell having an internal accommodating space, the detection device being disposed within the accommodating space of the inner shell, and the outer shell being disposed outside the inner shell.

[0009] Furthermore, the outer shell includes an upper shell and a lower shell, which are respectively fitted onto the upper and lower parts of the inner shell.

[0010] Furthermore, it also includes foot pads, which are rectangularly distributed on the bottom surface of the lower shell.

[0011] Furthermore, a miniature printer is also installed on the upper casing.

[0012] Furthermore, the detection device also includes:

[0013] The substrate is used to support the components. The substrate is connected to the optical box detection components, as well as the lead screw stepper motor, motor mounting base, linear guide rail, zero-point reset photoelectric sensor, reagent card compartment assembly, detection component sheet metal, barcode scanning module, and reader / writer.

[0014] Furthermore, the substrate is provided with multiple motherboard mounting posts, and the main control board is mounted on top of the multiple motherboard mounting posts.

[0015] Furthermore, the reagent card compartment assembly includes a card compartment base, a card compartment top cover, a lens cover, and a plano-convex cylindrical mirror. The card compartment base is fixedly connected to a linear guide rail, and the card compartment top cover is connected to the upper end of the card compartment base, forming a receiving space with the card compartment base for placing reagent cards. A plano-convex cylindrical mirror is provided on the side of the card compartment top cover near the card compartment base, and a lens cover is connected to the side of the card compartment top cover away from the card compartment base.

[0016] Furthermore, a battery is also provided on the substrate, which is fixed to the substrate by a battery fixing housing, and is used to power the various components of the single-channel fluorescence immunoassay analyzer.

[0017] Furthermore, a switch button is also provided on the housing, and a keypad is connected below the switch button. The keypad is connected to the internal circuitry to control the circuit switching of the single-channel fluorescence immunoassay analyzer.

[0018] This invention provides a single-channel fluorescence immunoassay analyzer that solves the problems of weakened effective signal value acquisition, significant loss of excitation and reception efficiency, and inability to achieve optimal signal-to-noise ratio during acquisition in existing equipment. It improves the sensitivity of the fluorescence immunoassay analyzer, increases the signal value of fluorescence acquisition, enhances the signal-to-noise ratio, and can provide more accurate detection results when detecting low-concentration samples. Attached Figure Description

[0019] The accompanying drawings, which form part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an undue limitation of the present invention. In the drawings:

[0020] Figure 1 This is a three-dimensional assembly drawing of a single-channel fluorescence immunoassay analyzer according to the present invention.

[0021] Figure 2 This is a three-dimensional assembly drawing of the detection device of a single-channel fluorescence immunoassay analyzer according to the present invention.

[0022] Figure 3 This is a three-dimensional assembly diagram of the reagent card compartment of a single-channel fluorescence immunoassay analyzer according to the present invention.

[0023] Figure 4 This is a three-dimensional assembly drawing of the optical box detection component of a single-channel fluorescence immunoassay analyzer according to the present invention.

[0024] Figure 5 This is a detection optical path diagram of a single-channel fluorescence immunoassay analyzer according to the present invention.

[0025] The above figures include the following reference numerals:

[0026] 01. Detection device; 02. Lower housing; 03. Inner liner housing; 04. Switch button; 05. Display screen; 06. Upper housing; 07. Mini printer; 08. Button board; 09. Foot pads; 101. Main control board; 102. Main board mounting post; 103. Base plate; 104. Lead screw stepper motor; 105. Reagent card; 106. Linear guide rail; 107. Battery; 108. Battery mounting housing; 109. Zero-point reset photoelectric sensor; 110. Reagent card compartment assembly; 111. Sheet metal of detection assembly; 11 2. Optical box detection assembly; 113. Barcode scanning module; 114. Reader / writer; 115. Reagent card; 1101. Card compartment base; 1102. Card compartment cover; 1103. Lens cover plate; 1104. Plano-convex cylindrical mirror; 1121. Left housing of optical box; 1122. Right housing of optical box; 1123. LED light board; 1124. Receiver board assembly; 1125. First plano-convex lens; 1126. Second plano-convex lens; 1127. Dichroic mirror; 1128. Third plano-convex lens; 1129. Filter. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present utility model or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0028] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0029] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of this invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0030] The following is for reference. Figures 1 to 5 The present invention will be further described below:

[0031] A single-channel fluorescence immunoassay analyzer, characterized in that it comprises: a detection device 01, a housing, a switch button 04, and a display screen 05, wherein the housing has a receiving cavity, the detection device 01 is installed in the receiving cavity of the housing, and the display screen 05 is installed on the top of the housing;

[0032] In this embodiment, the housing includes an outer shell and an inner shell 03. The inner shell 03 has an internal accommodating space. The detection device 01 is disposed in the accommodating space of the inner shell 03. The outer shell is disposed outside the inner shell 03. The outer shell includes an upper shell 06 and a lower shell 02, which are respectively fitted onto the upper and lower parts of the inner shell 03.

[0033] In this embodiment, a switch button 04 is also provided on the housing, and a button board 08 is connected below the switch button. The button board 08 is connected to the internal circuit to control the circuit on and off of the single-channel fluorescence immunoassay analyzer.

[0034] The detection device 01 is equipped with a light box detection assembly 112, which includes a left light box housing 1121 and a right light box housing 1122. The left light box housing 1121 and the right light box housing 1122 cooperate to form an accommodating space. An LED light panel 1123 is arranged inside the accommodating space to provide excitation light for detection. A first plano-convex lens 1125 and a dichroic mirror 1127 are arranged sequentially on one side of the lower end of the LED light panel 1123. A second plano-convex lens 1126 is arranged below the dichroic mirror 1127. A third plano-convex lens 1128 is arranged above the dichroic mirror 1127. A filter 1129 is arranged above the third plano-convex lens 1128. A receiving plate assembly 1124 is arranged above the filter 1129.

[0035] The diameter of the second plano-convex lens 1126 is larger than the diameter of the plano-convex cylindrical lens 1104.

[0036] The substrate 103 is used to support the components. The substrate 103 is connected to the optical box detection component 112, as well as the lead screw stepper motor 104, the motor mounting base 105, the linear guide rail 106, the zero-point reset photoelectric sensor 109, the reagent card compartment component 110, the detection component sheet metal 111, the barcode scanning module 113, and the reader / writer 114.

[0037] In this embodiment, a plurality of motherboard fixing posts 102 are also provided on the substrate 103, and a main control board 101 is provided above the plurality of motherboard fixing posts 102.

[0038] In this embodiment, the reagent card compartment assembly 110 includes a card compartment base 1101, a card compartment cover 1102, a lens cover 1103, and a plano-convex cylindrical mirror 1104. The card compartment base 1101 is fixedly connected to the linear guide rail 106. The card compartment cover 1102 is connected to the upper end of the card compartment base 1101 and cooperates with the card compartment base 1101 to form a receiving space for placing the reagent card 115. The plano-convex cylindrical mirror 1104 is provided on the side of the card compartment cover 1102 near the card compartment base 1101, and the lens cover 1103 is connected to the side of the card compartment cover 1102 away from the card compartment base 1101.

[0039] The testing device includes the following functions: a reader / writer 114 reads the standard curve of the reagent kit; a barcode scanning module 113 automatically identifies different items on the reagent card 115; a reagent card compartment assembly 110 loads the reagent cards and provides a darkroom testing environment, while also optimizing the optical path in conjunction with the optical box detection assembly 112; a lead screw stepper motor 104, a linear guide rail 106, and a zero-point reset photoelectric sensor 109 work together with the reagent card compartment assembly 110 to achieve high-precision and stable operation of the reagent cards; a battery 107 enables the instrument to be used for portable testing; and the left and right housings of the optical box 1121 and 1122 serve as the fixing carriers and encapsulation for the internal components.

[0040] LED light panel 1123 provides excitation light for detection; first plano-convex lens 1125, second plano-convex lens 1126 and third plano-convex lens 1128 together correct the optical path; dichroic mirror 1127 distinguishes and isolates excitation light and fluorescence; filter 1129 filters out stray light and reduces background noise; receiver assembly 1124 is used to receive fluorescence signal.

[0041] In the optical path, a plano-convex cylindrical mirror 1104 is used to focus and optimize the circular excitation light spot emitted by the photocell detection component into a highly efficient rectangular light spot, which perfectly covers the detection line area in the reagent card. This effectively reduces the area of ​​the light spot illuminating areas other than the detection line area, thus minimizing the area with high background noise. At the same time, when receiving signals, the plano-convex cylindrical mirror 1104 also maximizes the reception of fluorescence signals, greatly improving the signal-to-noise ratio of fluorescence acquisition.

[0042] In this embodiment, foot pads 09 and 03 are also included. The foot pads 03 are rectangularly distributed on the bottom surface of the lower housing 02. They can prevent tabletops and other surfaces from being worn due to long-term friction with the lower housing 02, and also provide some support.

[0043] In this embodiment, a micro printer 07 is also installed on the upper housing 06.

[0044] In this embodiment, a battery 107 is also provided on the substrate 103. The battery 107 is fixed to the substrate 103 by a battery fixing housing 108 and is used to power the components of the single-channel fluorescence immunoassay analyzer.

[0045] During testing, the reagent card 115 is placed in the sample application port, and then inserted into the instrument's reagent card compartment. The "Test" button is then clicked on the display screen 05. The lead screw stepper motor 104 drives the reagent card compartment assembly 110 into the instrument. The barcode scanning module 113 automatically identifies the test items on the reagent card 115. The reagent card 115 continues to move into the instrument. When the test line moves near the second plano-convex lens 1126 of the optical box detection assembly 112, the instrument acquires a signal. The test stops when the reagent card compartment assembly 110 moves to the zero-point reset photoelectric sensor 109. Then, the reagent card compartment assembly 110 reverses its movement, and the reagent card 115 exits from the instrument's port. The display screen 05 shows the test results. A test report can be randomly printed using the micro printer 07. Finally, the reagent card 115 is removed, completing the test.

[0046] This application achieves a high degree of integration of multi-functional modules, including sample information entry, reagent kit information entry, standard curve recognition and entry, one-click detection results, detection completion report printing, and rechargeability.

[0047] In the description of this utility model, it should be understood that the directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this utility model. The directional terms "inner" and "outer" refer to the inner and outer contours of each component itself.

[0048] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A single-channel fluorescence immunoassay analyzer, characterized in that, include: The device includes a detection device (01), a housing, a switch button (04), and a display screen (05). The housing has a receiving cavity, the detection device (01) is installed in the receiving cavity of the housing, and the display screen (05) is installed on the top of the housing. The detection device (01) is provided with a light box detection assembly (112), which includes a left light box housing (1121) and a right light box housing (1122). The left light box housing (1121) and the right light box housing (1122) cooperate to form an accommodating space. An LED light board (1123) is provided inside the accommodating space to provide excitation light for detection. A first plano-convex lens (1125) and a dichroic mirror (1127) are arranged sequentially on one side of the lower end of the LED light board (1123). A second plano-convex lens (1126) is arranged below the dichroic mirror (1127). A third plano-convex lens (1128) is arranged above the dichroic mirror (1127). A filter (1129) is arranged above the third plano-convex lens (1128). A receiving plate assembly (1124) is arranged above the filter (1129).

2. The single-channel fluorescence immunoassay analyzer according to claim 1, characterized in that, The housing includes an outer shell and an inner shell (03). The inner shell (03) has an internal accommodating space. The detection device (01) is disposed in the accommodating space of the inner shell (03). The outer shell is disposed outside the inner shell (03).

3. A single-channel fluorescence immunoassay analyzer according to claim 2, characterized in that, The outer shell includes an upper shell (06) and a lower shell (02), which are respectively fitted onto the upper and lower parts of the inner shell (03).

4. A single-channel fluorescence immunoassay analyzer according to claim 3, characterized in that, It also includes foot pads (09), which are rectangularly distributed on the bottom surface of the lower housing (02).

5. A single-channel fluorescence immunoassay analyzer according to claim 3, characterized in that, A micro printer (07) is also installed on the upper housing (06).

6. A single-channel fluorescence immunoassay analyzer according to claim 1, characterized in that, The detection device (01) further includes: A substrate (103) is used to support the assembly. The substrate (103) is connected to the light box detection assembly (112), as well as a lead screw stepper motor (104), a motor mounting base (105), a linear guide rail (106), a zero-point reset photoelectric sensor (109), a reagent card compartment assembly (110), a detection assembly sheet metal (111), a barcode scanning module (113), and a reader (114).

7. A single-channel fluorescence immunoassay analyzer according to claim 6, characterized in that, The substrate (103) is also provided with a plurality of motherboard fixing posts (102), and a main control board (101) is provided above the plurality of motherboard fixing posts (102).

8. A single-channel fluorescence immunoassay analyzer according to claim 6, characterized in that, The reagent card compartment assembly (110) includes a card compartment base (1101), a card compartment cover (1102), a lens cover (1103), and a plano-convex cylindrical mirror (1104). The card compartment base (1101) is fixedly connected to the linear guide rail (106). The card compartment cover (1102) is connected to the upper end of the card compartment base (1101) and cooperates with the card compartment base (1101) to form a receiving space for placing reagent cards (115). The plano-convex cylindrical mirror (1104) is provided on the side of the card compartment cover (1102) close to the card compartment base (1101), and the lens cover (1103) is connected to the side of the card compartment cover (1102) away from the card compartment base (1101).

9. A single-channel fluorescence immunoassay analyzer according to claim 6, characterized in that, A battery (107) is also provided on the substrate (103). The battery (107) is fixed to the substrate (103) by a battery fixing housing (108) and is used to power the components of the single-channel fluorescence immunoassay analyzer.

10. A single-channel fluorescence immunoassay analyzer according to claim 3, characterized in that, The housing is also provided with a switch button (04), and a keypad (08) is connected below the switch button. The keypad (08) is connected to the internal circuit and controls the circuit on / off of the single-channel fluorescence immunoassay analyzer.

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