Rapid detection kit for gastric cancer nerve invasion

Abstract

The utility model belongs to the technical field of biomedical detection, and discloses a rapid detection kit for gastric cancer nerve invasion, which comprises a cover plate hinged to the top of a kit body, and a sampling tube placing rack is embedded in the top of the kit body. Three first placing cavities, three second placing cavities and a third placing cavity are formed in the top of the kit body, a plurality of placing grooves are formed in the top of the sampling tube placing frame, collecting tubes are arranged in the placing grooves, each collecting tube comprises a first dropper fixedly connected to the center of the bottom of a tube body, and a second dropper fixedly connected to the center of the bottom of the tube body. The top of the tube body is in threaded connection with a tube cover, and a first cavity is formed in the first dropper. Complex equipment and operation steps are not needed, clinical application is facilitated, the time from sample collection to result output is greatly shortened, markers with extremely low concentration can be detected, the detection rate of early gastric cancer nerve invasion is increased, false positive and false negative results are reduced, and the detection accuracy is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the biomedical detection technical field, is quick detection reagent box of gastric cancer nerve invasion in concrete. BACKGROUND

[0002] Gastric cancer is one of the common malignant tumors in the world, and its prognosis is closely related to the invasiveness and metastatic ability of tumor. Nerve invasion is an important marker of the increasing invasiveness of gastric cancer, and early detection of nerve invasion of gastric cancer is of great significance for guiding clinical treatment and predicting prognosis.

[0003] However, in order to detect whether gastric cancer has nerve invasion, only pathological detection can be carried out at present, by obtaining gastric cancer tissue, fixing, then packing into wax block, slicing, after HE staining, observing whether gastric cancer has invaded nerve through microscope. On this basis, further determine whether gastric cancer has invaded nerve by immunohistochemistry on the slice and dyeing specific markers. The steps are very complicated, and it takes a long time, about one to two weeks to complete, and the existing detection method has problems of complex operation, long detection time, insufficient sensitivity and specificity. UTILITY MODEL CONTENT

[0004] In order to improve the problems of complex operation, long detection time, insufficient sensitivity and specificity of the existing detection method, the utility model provides a quick detection reagent box of gastric cancer nerve invasion.

[0005] In order to achieve the above purpose, the utility model provides the following technical scheme: a kind of quick detection reagent box of gastric cancer nerve invasion, including the cover plate of being hinged and installed with the top of reagent box body, the sampling tube placing rack is embedded in the top of reagent box body, the top of reagent box body is respectively provided with three placing cavities one, three placing cavities two and one placing cavity three, the top of sampling tube placing rack is provided with a plurality of placing grooves, a plurality of placing grooves are equipped with collection tube, the collection tube includes the dropper one of being fixedly connected in the central bottom of tube body, the tube body top is connected with tube cover, the dropper one is provided with cavity one, the cavity one is equipped with valve body assembly for cutting off the flow of dropper one, the valve body assembly includes the through hole of being passed through and being provided in the central top of ball, the ball is fixedly installed in the intermediate spherical cavity of cavity one, the flow limiting column for blocking through hole is slidably arranged in the ball, the flow limiting column top is equipped with trapezoidal clamping block;

[0006] Three placing cavities one are respectively equipped with lysis buffer, detection reagent body and color developing agent, three placing cavities two are all equipped with rubber head dropper.

[0007] Preferably, one side of the ball is provided with a sliding hole in the middle, the drip pipe is provided with a sliding groove two corresponding to the sliding hole, the flow limiting column extends into the sliding hole through the sliding groove two, and the flow limiting column slides transversely in the sliding hole.

[0008] Preferably, the top of the flow limiting column away from the handle is provided with a sliding groove one, the trapezoidal clamping block is located in the sliding groove one and longitudinally slides, the trapezoidal clamping block is fixedly connected with a spring one at the bottom of the sliding groove one, and the ball is provided with two clamping grooves one in the middle and matched with the shape of the trapezoidal clamping block.

[0009] Preferably, the drip pipe is provided with a cavity two communicated with the sliding groove two, and the cavity two is provided with a limiting component limiting the flow limiting column to slide in the sliding hole.

[0010] Preferably, the top of the friction block is fixedly connected with a spring two at the top of the cavity two, the middle of the friction block is provided with an inclined groove, the drip pipe is movably penetrated with a push rod at a position corresponding to the inclined groove, the inner end of the push rod extends into the inclined groove, the top of the push rod is fixedly provided with a connecting plate, the drip pipe is provided with a sliding groove three matched with the shape of the connecting plate, the connecting plate is located in the sliding groove three and transversely slides, and the inner side of the connecting plate is fixedly connected with a spring three at the inner end of the sliding groove three.

[0011] Preferably, the bottom of the push rod is fixedly connected with a connecting block, the inner side of the connecting block is fixedly connected with an inserting block, the side of the drip pipe is provided with a pressing slot matched with the shape of the inserting block, the bottom of the inserting block is provided with a sliding groove four, the sliding groove four is slidably provided with a right-angled triangular clamping block, the top of the right-angled triangular clamping block is fixedly connected with a spring four at the top of the sliding groove four, and the bottom of the pressing slot is provided with a clamping groove two matched with the shape of the right-angled triangular clamping block.

[0012] Preferably, the top of the right-angled triangular clamping block is fixedly connected with a lever, the inserting block and the connecting block are provided with a sliding groove five matched with the shape of the lever, and the lever extends to the outside through the sliding groove five.

[0013] Compared with the prior art, the ball drip irrigation device has the advantages that:

[0014] The utility model discloses a stomach cancer tissue sample is collected to the collection pipe, adds lysing buffer, and the specific marker PSAP of gastric cancer occurrence nerve invasion is released to the cell lysis, mixes the sample after lysis with detection reagent body, carries out specific binding reaction, adds color developing agent, carries out signal amplification and visualization, according to the determination standard in the specification, reads and records the detection result, does not need complicated equipment and operation step, and it is convenient for clinical application, and the time of sample collection to result output is greatly shortened. Can detect the marker of extremely low concentration, improve the detection rate of early gastric cancer nerve invasion, reduce false positive and false negative result, improve the accuracy of detection. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the whole shaft measurement front view structure schematic diagram of the utility model;

[0016] Figure 2 It is the box body structure plan view of the utility model;

[0017] Figure 3 It is the collection pipe structure schematic diagram of the utility model;

[0018] Figure 4 It is the valve body assembly structure schematic diagram of the utility model;

[0019] Figure 5 It is the dropper one structure schematic diagram of the utility model;

[0020] Figure 6 It is the ball section structure schematic diagram of the utility model;

[0021] Figure 7 It is the valve body assembly local structure schematic diagram of the utility model;

[0022] Figure 8 It is the limiting assembly structure schematic diagram of the utility model;

[0023] Figure 9 It is Figure 8 The structure enlarged schematic diagram of A place in the middle.

[0024] In the drawing: 1, reagent box body; 2, cover plate; 3, sampling tube placing rack; 4, placing cavity one; 5, placing cavity two; 6, placing cavity three; 7, placing groove;

[0025] 8, collection pipe; 81, pipe body; 82, pipe cover; 83, dropper one;

[0026] 84, valve body assembly; 841, ball; 842, through -hole; 843, sliding hole; 844, flow limiting column; 845, handle; 846, sliding slot one; 847, trapezoidal clamping block; 848, spring one; 849, clamping groove one;

[0027] 85. Limiting component; 851. Friction block; 852. Spring 2; 853. Inclined groove; 854. Push rod; 855. Slide groove 3; 856. Connecting plate; 857. Spring 3; 858. Connecting block; 859. Insertion block; 860. Push slot; 861. Slide groove 4; 862. Right-angled triangular locking block; 863. Spring 4; 864. Toggle lever; 865. Slide groove 5; 866. Locking groove 2;

[0028] 86. Cavity 1; 87. Cavity 2; 88. Slide 2;

[0029] 9. Dropper; 10. Lysis buffer; 11. Test reagent; 12. Chromogenic reagent; 13. Instructions for use. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] like Figures 1 to 9 As shown, this utility model provides a rapid detection kit for gastric cancer nerve invasion, including a cover plate 2 hinged to the top of the kit body 1. A sampling tube holder 3 is embedded in the top of the kit body 1. The top of the kit body 1 has three placement cavities 4, three placement cavities 5, and one placement cavity 6. The top of the sampling tube holder 3 has several placement slots 7 arranged in a rectangular array. Each of the placement slots 7 contains a collection tube 8 for collecting gastric cancer tissue samples. The shape of the placement slot 7 is adapted to the shape of the collection tube 8. The inner wall of the placement slot 7 abuts against the surface of the collection tube 8 to avoid misalignment and prevent the kit body 1 from shaking. When the sample collection tube 8 moves, it shakes in the placement slot 7. The three placement chambers 1 and 4 are respectively equipped with lysis buffer 10 for lysing cells in the sample and releasing the test marker PSAP, test reagent body 11 containing antibody or nucleic acid probe that specifically binds to PSAP, a marker related to gastric cancer nerve invasion, and chromogenic agent 12 for signal amplification and visualization after the reaction. The three placement chambers 2 and 5 are each equipped with a dropper 9, which is used to draw lysis buffer 10, test reagent body 11 and chromogenic agent 12, respectively. The three placement chambers 3 and 6 are equipped with instruction manual 13, which details the usage and operation steps of the kit.

[0032] like Figures 3-7As shown, the collection tube 8 includes a dropper one 83 fixedly connected at the bottom center of the tube body 81, the tube cover 82 is threadedly connected at the top of the tube body 81, the cavity one 86 is opened in the dropper one 83, the cavity one 86 is in the shape of a sandglass, facilitating the flow of reagents, the valve body assembly 84 is arranged in the cavity one 86 for cutting off the flow of the dropper one 83, the valve body assembly 84 includes a through hole 842 opened through the top center of the ball 841, the ball 841 is fixedly installed in the spherical cavity in the middle of the cavity one 86, the flow limiting column 844 is slidably arranged in the ball 841 for blocking the through hole 842, the trapezoidal clamping block 847 is arranged at the top of the flow limiting column 844, the sliding hole 843 is opened at the center of one side of the ball 841, the sliding groove two 88 is opened at the position corresponding to the sliding hole 843 of one side of the dropper one 83 and is in communication with the sliding hole 843, the inner end of the flow limiting column 844 extends into the sliding hole 843 through the sliding groove two 88, the flow limiting column 844 realizes transverse sliding in the sliding hole 843, the handle 845 is fixedly connected to the end of the flow limiting column 844 away from the ball 841, the sliding groove one 846 is opened at the top of the end of the flow limiting column 844 away from the handle 845, the trapezoidal clamping block 847 is located in the sliding groove one 846 and realizes longitudinal sliding, the spring one 848 is fixedly connected to the bottom of the sliding groove one 846 and is fixedly connected to the end of the trapezoidal clamping block 847, the two clamping grooves one 849 are opened in the ball 841 and are matched with the shape of the trapezoidal clamping block 847.

[0033] As Figure 8 , Figure 9As shown, the cavity two 87 is communicated with the chute two 88 in the dropper one 83, the limiting assembly 85 for limiting the sliding of the flow limiting column 844 in the sliding hole 843 is arranged in the cavity two 87, the limiting assembly 85 includes the friction block 851 for longitudinal sliding in the cavity two 87, the side of the friction block 851 in surface contact with the flow limiting column 844 abuts against the surface of the flow limiting column 844, so that the virtual position is avoided, and the friction between the friction block 851 and the flow limiting column 844 is increased, the spring two 852 is fixedly connected to the top of the friction block 851, one end of the spring two 852 away from the friction block 851 is fixedly connected to the top of the cavity two 87, the inclined groove 853 is arranged in the side of the friction block 851, the push rod 854 is movably and penetratively arranged at the position corresponding to the inclined groove 853 of the dropper one 83, the inner end of the push rod 854 extends into the inclined groove 853, the connecting plate 856 is fixedly arranged on the top of the push rod 854, the chute three 855 is arranged in the dropper one 83 and is matched with the shape of the connecting plate 856, the connecting plate 856 is arranged in the chute three 855 and is horizontally slid, the spring three 857 is fixedly connected to the inner side of the connecting plate 856, one end of the spring three 857 away from the connecting plate 856 is fixedly connected to the inner end of the chute three 855, the connecting block 858 is fixedly connected to the bottom of the push rod 854, the insertion block 859 is fixedly connected to the inner side of the connecting block 858, the press insertion groove 860 is arranged in the side of the dropper one 83 and is matched with the shape of the insertion block 859, the chute four 861 is arranged in the bottom of the insertion block 859, the right-angled triangular clamping block 862 is slidably arranged in the chute four 861, the spring four 863 is fixedly connected to the top of the right-angled triangular clamping block 862, one end of the spring four 863 away from the right-angled triangular clamping block 862 is fixedly connected to the top of the chute four 861, the clamping groove two 866 is arranged in the bottom of the press insertion groove 860 and is matched with the shape of the right-angled triangular clamping block 862, the push rod 864 is fixedly connected to the top of the right-angled triangular clamping block 862, the chute five 865 is arranged in the insertion block 859 and the connecting block 858 and is matched with the shape of the push rod 864, and the outer end of the push rod 864 extends to the outside through the chute five 865.

[0034] The rubber head dropper 9, the lysis buffer 10, the detection reagent body 11, the cover glass, the microscope and the color developing agent 12 are prior art, and the structures or components will not be described in detail.

[0035] Principle and process of the utility model:

[0036] In use, unscrew the tube cover 82 at the top of the tube body 81, after collecting the gastric cancer tissue sample, use the rubber bulb dropper 9 containing the lysis buffer 10 to suck the lysis buffer 10 into the tube body 81, lyse the cells and release the specific marker PSAP of gastric cancer, then use the rubber bulb dropper 9 containing the detection agent body 11 to suck the detection agent body 11 and mix it with the lysed sample, carry out specific binding reaction, then use the rubber bulb dropper 9 containing the chromogenic agent 12 to suck the chromogenic agent 12 and add it into the sample, carry out signal amplification and visualization, then screw on the tube cover 82, then press the push rod 854, the push rod 854 extends into the inclined groove 853, the insertion block 859 is inserted into the pressing slot 860, and the right triangle clamping block 862 is clamped in the second clamping groove 866. The push rod 854 is fixed at this position and cannot rebound under the action of the spring three 857. The operator can release his hand pushing the push rod 854. The push rod 854 applies a pushing force to the inclined surface of the inclined groove 853 of the friction block 851. As can be seen from the force decomposition, the friction block 851 will be subjected to a force in the direction of the second cavity 87. Then the friction block 851 slides into the second cavity 87 to compress the second spring 852. At this time, the friction block 851 is no longer in surface contact with the flow limiting column 844, so there is no friction force between the friction block 851 and the flow limiting column 844. At this time, the flow limiting column 844 can slide in the sliding hole 843. The operator pulls the handle 845, and the flow limiting column 844 slides outward in the sliding hole 843. The inclined surface of the trapezoidal clamping block 847 is stressed during the sliding process. As can be seen from the force decomposition, the trapezoidal clamping block 847 is subjected to a force in the direction of the first sliding groove 846. The trapezoidal clamping block 847 slides into the first sliding groove 846 and then slides out of the first clamping groove 849. When the flow limiting column 844 slides in the sliding hole 843 to the position where the trapezoidal clamping block 847 corresponds to the other first clamping groove 849, the trapezoidal clamping block 847 rebounds and is clamped in the first clamping groove 849 under the action of the first spring 848. At this time, stop pulling the flow limiting column 844. At this time, the through hole 842 will no longer be blocked by the flow limiting column 844, and the sample can flow out of the through hole 842. Then it drips on the cover glass. When the dripping ends, push the flow limiting column 844 through the handle 845. The flow limiting column 844 slides in the sliding hole 843 to the position where the trapezoidal clamping block 847 is clamped in the first clamping groove 849 at the inner end. At this time, the flow limiting column 844 blocks the through hole 842 to prevent the sample liquid from dripping out of the dropper 83. Then turn the lever 864. The lever 864 drives the right triangle clamping block 862 to slide into the fourth sliding groove 861. At this time, the right triangle clamping block 862 is no longer clamped in the second clamping groove 866, and the push rod 854 rebounds to the original position under the action of the third spring 857. The pushing force acting on the friction block 851 disappears, and the friction block 851 rebounds to the original position under the action of the second spring 852. The friction block 851 is in surface contact with the flow limiting column 844, and there is a friction force between the friction block 851 and the flow limiting column 844, which prevents the flow limiting column 844 from sliding in the sliding hole 843 and opens the through hole 842,After the sample is dropped from the dropper 83, the cover glass on which the sample is dropped is placed on the microscope to be observed, and the detection result is read and recorded according to the judgment criteria in the instruction manual 13.

[0037] It should be noted that the relational terms herein such as first and second and the like are used solely to distinguish one entity or action from another, without necessarily requiring or implying any such actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0038] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A rapid detection kit for nerve invasion in gastric cancer, comprising a cover plate (2) hinged to the top of a kit body (1), wherein a sampling tube holder (3) is embedded in the top of the kit body (1), and the top of the kit body (1) is provided with three placement cavities one (4), three placement cavities two (5), and one placement cavity three (6), characterized in that: The sampling tube rack (3) is provided with a plurality of placing grooves (7) on the top, a plurality of collecting tubes (8) are arranged in the placing grooves (7), the collecting tube (8) comprises a dropper I (83) fixedly connected to the bottom center of a tube body (81), a tube cover (82) is threadedly connected to the top of the tube body (81), a cavity I (86) is formed in the dropper I (83), and a valve body assembly (84) for cutting off the flow of the dropper I (83) is arranged in the cavity I (86). Three placing cavities I (4) are respectively provided with a lysis buffer (10) for releasing a to-be-tested marker PSAP, a detection reagent body (11) containing an antibody or nucleic acid probe specifically combined with a gastric cancer nerve invasion related marker PSAP, and a chromogenic agent (12) for signal amplification and visualization after detection reaction.

2. The rapid detection kit for gastric cancer nerve invasion according to claim 1, characterized by: A sliding hole (843) is formed in the side center of the ball (841), a sliding groove II (88) in communication with the sliding hole (843) is formed in the side of the dropper I (83) corresponding to the position of the sliding hole (843), the inner end of the flow limiting column (844) extends into the sliding hole (843) through the sliding groove II (88), and the flow limiting column (844) realizes transverse sliding in the sliding hole (843), and a handle (845) is fixedly connected to the end of the flow limiting column (844) away from the ball (841).

3. The rapid detection kit for the neural invasion of gastric cancer according to claim 2, characterized by: A sliding groove I (846) is formed in the top of the end of the flow limiting column (844) away from the handle (845), the trapezoidal clamping block (847) is located in the sliding groove I (846) and realizes longitudinal sliding, a spring I (848) is fixedly connected to the bottom of the trapezoidal clamping block (847) and fixedly connected to the groove cavity bottom of the sliding groove I (846), and two clamping grooves I (849) matched with the shape of the trapezoidal clamping block (847) are formed in the ball (841).

4. The rapid detection kit for the neural invasion of gastric cancer according to claim 3, characterized by: A cavity II (87) in communication with the sliding groove II (88) is formed in the dropper I (83), a limiting assembly (85) for limiting the sliding of the flow limiting column (844) in the sliding hole (843) is arranged in the cavity II (87), and the limiting assembly (85) comprises a friction block (851) arranged in the cavity II (87) and realizing longitudinal sliding.

5. The rapid detection kit for gastric cancer nerve invasion according to claim 4, characterized by: The top of the friction block (851) is fixed with spring two (852) whose top is fixed in the inner cavity of cavity two (87), one side of the friction block (851) is provided with inclined groove (853) in the center, the push rod (854) is movably penetrated and installed at the position corresponding to the inclined groove (853) of one side of the dropper one (83), the inner end of the push rod (854) extends into the inclined groove (853), the connecting plate (856) is fixed on the top of the push rod (854), the sliding groove three (855) with the same shape as the connecting plate (856) is formed in the dropper one (83), the connecting plate (856) is located in the sliding groove three (855) and realizes transverse sliding, the spring three (857) whose inner end is fixed in the inner end of the sliding groove three (855) is fixed on the inner side of the connecting plate (856).

6. The rapid detection kit for gastric cancer nerve invasion according to claim 5, characterized by: The bottom of the push rod (854) is fixed with the connecting block (858), the inner side of the connecting block (858) is fixed with the insertion block (859), the press insertion groove (860) with the same shape as the insertion block (859) is formed in the side of the dropper one (83), the sliding groove four (861) is formed in the bottom of the insertion block (859), the right-angled triangular clamping block (862) is slidably installed in the sliding groove four (861), the spring four (863) whose top is fixed in the groove cavity of the sliding groove four (861) is fixed on the top of the right-angled triangular clamping block (862), the clamping groove two (866) with the same shape as the right-angled triangular clamping block (862) is formed in the groove cavity of the press insertion groove (860).

7. The rapid detection kit for gastric cancer nerve invasion according to claim 6, characterized by: The top of the right-angled triangular clamping block (862) is fixed with the lever (864), the sliding groove five (865) with the same shape as the lever (864) is formed in the insertion block (859) and the connecting block (858), one end of the lever (864) away from the right-angled triangular clamping block (862) extends to the outside through the sliding groove five (865).

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