Pollution-free continuous culture system for micrococcus

Abstract

The utility model discloses a pollution-free continuous culture system for micrococcus, which comprises a culture container, the culture container is communicated with a water return pipe, a gas supply system is arranged near the joint of the water return pipe and the culture container, the gas supply system is communicated with the water return pipe, the bottom of the culture container is provided with a connecting pipe, and the connecting pipe is communicated with the water return pipe. A switch valve is arranged on the connecting pipe, the other tail end of the connecting pipe is connected with a nutrition circulating system, and the nutrition circulating system is connected with the top of the culture container. The gas device and the recovery pipe are mounted on the outer side of the culture main body, so that internal and external circulation of nannochlorella and culture solution is realized, nutrient substances, gas and light are uniformly distributed, after microalgae are cultured for a certain time, the culture solution is subjected to impurity removal and sterilization treatment and then returns to a culture container again, antibiotics are not added in the whole process, and pollution is avoided; no tail water is discharged, the operation is simple, and large-scale production is easy.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the aquaculture technical field, concretely relates to a continuous type, pollutionless culture system for nannochloropsis. BACKGROUND

[0002] Microalgae are a class of photosynthetic autotrophic single-cell organisms, which have the characteristics of abundant resources, various species, high photosynthetic efficiency, fast growth, and strong adaptability. They are rich in protein, lipid, carbohydrate, vitamins, minerals, and various bioactive substances, which are essential for maintaining fish health. Compared with terrestrial crops and animal feed, microalgae have more advantages in nutritional composition and do not compete with the human food chain. Algae can be used as food for rotifers, copepods, and brine shrimp, and these organisms can carry pigments such as HUFA and lutein / astaxanthin into fish bodies. On the other hand, algae can be added to fish fry ponds to provide shade, maintain the nutritional value of animal feed, and provide algal cell vitamins, similar to environmental probiotics. The growth rate of fish fry varies depending on the use of algae or not at different stages, indicating that microalgae play an important role in fish fry culture. Therefore, adding algae water to the fry pond can improve the growth and survival of fish fry.

[0003] However, the cultivation of microalgae not only requires professional cultivation facilities, but also has technical bottlenecks such as complex operation, high production cost, and low survival rate of microalgae in large-scale cultivation. Common problems include: (1) uneven light intensity causes some algal cells to receive insufficient or excessive light, and if the light in some areas is too strong, it will cause light inhibition of algal cells, reducing photosynthetic efficiency; while in areas with weak light, algal cells grow slowly. The stability of indoor algae cultivation light sources is poor, and flickering or brightness changes easily occur, causing stress reactions in algal cells. (2) Uneven stirring during microalgae cultivation can cause uneven distribution of nutrients and gases in the algal liquid. For example, insufficient or unstable supply of gases or carbon dioxide, and unreasonable aeration methods, affect photosynthesis and cell growth. (3) Open cultivation is prone to contamination, introducing bacteria or impurities, and causing feed contamination during fish fry hatching, resulting in economic losses for farmers after feeding fish fry. SUMMARY

[0004] In order to solve the above technical problems, the utility model provides a kind of for parachlorella, and the whole process is not added antibiotic, pollution-free, no tail water discharge, simple operation, easy to scale production.

[0005] The technical scheme provided by the utility model is as follows:

[0006] A kind of for parachlorella, cultivation container is communicated with backwater pipe, another end branch of backwater pipe has pipeline I and pipeline II, pipeline I is inserted into cultivation container upper side, pipeline II is used for exhaust, gas supply system is installed near the connecting place of backwater pipe and cultivation container, gas supply system is communicated with backwater pipe, for injecting gas into backwater pipe, create negative pressure environment, drive cultivation liquid to flow into backwater pipe and then flow back into cultivation container again;The bottom of cultivation container is equipped with connecting pipe, and the connecting pipe is equipped with switch valve, and another end of the connecting pipe is connected with nutrient circulation system, and the top of cultivation container is connected with nutrient circulation system.

[0007] Preferably, it further includes a heating pipe and a temperature control device, one end of the heating pipe is inserted into the cultivation container, and the other end is connected with the temperature control device outside the cultivation container.

[0008] Preferably, the nutrient circulation system includes a centrifugal filter, a transfer container and a sterilization device, the cultivation liquid flowing out from the bottom of the cultivation container enters the centrifugal filter, the parachlorella obtained by centrifugation is collected and used, the liquid after centrifugation enters the transfer container, and then enters the sterilization device for sterilization, and finally returns to the cultivation container.

[0009] Preferably, the gas supply system includes a gas pump, an air inlet pipe and a gas device gas supply outer cavity, the two ends of the air inlet pipe are respectively connected with the gas pump and the gas device gas supply outer cavity, the gas device gas supply outer cavity is fixed at the bottom position of the backwater pipe and is sealingly connected with the backwater pipe, a plurality of air inlet holes are arranged on the backwater pipe, the gas output from the gas pump enters the air inlet pipe, the gas device gas supply outer cavity and the backwater pipe in sequence.

[0010] Preferably, the top of the cultivation container is provided with a sealing cover, and the cover is made of transparent rigid glass or plastic film material.

[0011] More preferably, a plurality of air outlet holes are arranged on the cover.

[0012] Preferably, the main body of the cultivation container is made of plastic material, and the shape is conical.

[0013] Compared with the prior art, the utility model has the following technical advantages:

[0014] (1) the culture container in the utility model can be divided into two parts, the upper part is the expansion oxygen increasing area, the upper cover is equipped with exhaust hole, the lower part is the nutrient supply and gas supply circulation area, the gas supply system is arranged outside the culture container and is communicated with the bottom of the backwater pipe, the air or carbon dioxide is continuously sent into the backwater pipe (containing culture solution) through the pipeline by using the compressed air pump or fan, the gas rises in the liquid, and the pressure difference between the inside and outside of the bubble makes the bubble rise in the negative pressure environment around it, drives the surrounding water flow to form local turbulence, and then drives the culture solution containing paracoccus marcusii at the bottom of the culture container to rise into the backwater pipe and then flow back into the culture container, breaks the stratification of the water body in the culture container, promotes the mixing of the upper and lower water layers, improves the gas solubility rate in the deep water body, the culture solution containing paracoccus marcusii in the upper and lower parts of the culture container is continuously mixed, the paracoccus marcusii at the bottom is quickly circulated to the upper layer to receive light and carry out photosynthesis to achieve the purpose of proliferation and expansion.

[0015] (2) compared with the traditional open culture mode, the utility model adopts the closed culture mode, when the paracoccus marcusii reaches the collection period in the expansion concentration, the switch valve at the bottom of the culture container is opened, most of the culture solution containing paracoccus marcusii is discharged into the centrifugal filter to centrifuge, the high-concentration paracoccus marcusii after rapid collection is used, the filtered liquid is collected and sterilized, and then sent into the culture container for continuing to culture paracoccus marcusii. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the structure schematic view of the utility model

[0017] Figure 2 It is the structure schematic view of the culture container of the utility model

[0018] Figure 3 It is the structure schematic view of the gas supply system of the utility model

[0019] Wherein: 1, culture container 2, backwater pipe 3, pipeline I 4, pipeline II 5, air pump 6, air inlet pipe 7, gas device supply outer cavity 8, air inlet hole 9, switch valve 10, heating pipe 11, temperature control device 12, centrifugal filter 13, transfer container 14, sterilization device 15, upper cover. DETAILED DESCRIPTION

[0020] The utility model will be further explained in connection with specific embodiment and drawing.

[0021] As Figures 1-2 The utility model discloses a kind of pollution-free continuous cultivation systems for parachlorella, including culture container 1, backwater pipe 2, gas supply system, culture container 1 is communicated with backwater pipe 2, another end branch of backwater pipe 2 has pipeline I 3 and pipeline II 4, pipeline I 3 is inserted into culture container 1 upper, pipeline II 4 is used for exhaust, gas supply system includes air pump 5, air inlet pipe 6, gas device supply outer cavity 7, the both ends of air inlet pipe 6 are connected with air pump 5, gas device supply outer cavity 7 respectively, gas device supply outer cavity 7 is fixed at the bottom position of backwater pipe 2, and is sealingly connected with backwater pipe 2, backwater pipe 2 is equipped with several air inlet holes 8, the gas output from air pump 5, in turn into air inlet pipe 6, gas device supply outer cavity 7, then pass through air inlet hole 8 and enter backwater pipe 2, the gas in backwater pipe 2 creates negative pressure environment in its entering position vicinity, drive culture solution containing parachlorella to flow into backwater pipe 2 after again flowing back into culture container 1;The bottom of culture container 1 is equipped with connecting pipe, connecting pipe is equipped with switch valve 9, switch valve 9 is connected with nutrient circulation system, and nutrient circulation system is connected with the top of culture container 1.

[0022] The utility model sets up heating pipe 10 in the bottom of culture container 1, another end of heating pipe 10 is stretched out of culture container 1, and is connected with temperature control device 11, and the temperature in culture container 1 is controlled by the flow of hot water in heating pipe 10.The temperature of water is accurately controlled by temperature sensor transmission temperature controller, and the temperature is kept stable to meet the temperature requirement of different growth stages of parachlorella.

[0023] The utility model discloses nutrient circulation system includes centrifugal filter 12, transfer container 13, sterilization device 14, and culture solution containing parachlorella from the switch valve 9 of culture container 1 bottom enters centrifugal filter 12 in and is equipped with 1000-2000 mesh filter screen, and parachlorella obtained by centrifugation is collected and used, and the liquid after centrifugation enters transfer container 13, and the liquid in transfer container 13 contains nutrient solution, and after adjusting nutrient, it enters sterilization device 14 again, and after sterilization, it returns to culture container 1.

[0024] The utility model discloses a culture container 1 top is provided with sealed upper cover 15, is glass or transparent plastic film material, ensure the sealing property of device, prevent outside pollution, glass or transparent plastic film, ensure good light transmittance and will not release harmful substance.

[0025] The utility model discloses in actual use, inject culture container 1 new liquid needs full sterilization, kill the bacteria and other microorganisms that can enter culture container 1.

[0026] After the culture solution and the botryococcus are injected into the culture container 1, in the process of culture, a small part of the botryococcus and the culture solution will enter the backwater pipe 2 and the air inlet pipe 6, when the gas supply system is opened, the compressed air pump or the fan is used to continuously send the air or the carbon dioxide into the backwater pipe 2 through the air inlet pipe 6, the output gas has a certain pressure, which can press the botryococcus and the culture solution from the air inlet pipe 6 back into the backwater pipe 2, the backwater pipe 2 is vertically arranged and communicates with the culture container 1, the gas entering the backwater pipe 2 rises in the liquid in the backwater pipe 2, and the pressure difference between the inside and the outside of the formed bubbles causes a negative pressure environment around the bubbles during the rising process, drives the surrounding water flow to form a local turbulent flow, and then drives the culture solution with the botryococcus at the bottom of the culture container to flow into the backwater pipe 2 and then flow back into the culture container 1, so that the water layering formed by the botryococcus accumulated at the bottom in the culture process is broken, the upper and lower water layers are mixed, the gas dissolution rate of the deep water body is improved, the gas is continuously input, the upper and lower parts of the culture container 1 are cyclically mixed, the botryococcus at the bottom is quickly circulated to the upper layer, and receives light, so that all the botryococcus in the culture container 1 can absorb sufficient sunlight for photosynthesis to achieve the purpose of proliferation and expansion.

[0027] When the sample detection of the botryococcus in the material inlet reaches a certain density, the switch valve 9 at the bottom of the culture container 1 is opened, most of the culture solution containing the botryococcus in the culture container 1 flows into the centrifugal filter 12, the culture solution flows into the transfer container after centrifugation, is configured with nutrients, flows into the sterilization device 14 after sterilization, and is transported into the original culture container 1, so that the botryococcus liquid reserved in the original culture container 1 continues to expand the botryococcus, and the purpose of continuous expansion of the botryococcus is achieved.

[0028] The whole device is compact in design, simple to operate, can realize efficient expansion of the botryococcus, ensures that the whole process is pollution-free, and ensures the quality and yield of the botryococcus.

Claims

1. A pollution-free continuous propagation system for Nannochloropsis, characterized in that: The culture container is connected with a backwater pipe, the other end of the backwater pipe is branched into pipe I and pipe II, pipe I extends into the culture container, pipe II is used for exhausting, a gas supply system is installed near the connection of the backwater pipe and the culture container, the gas supply system is communicated with the backwater pipe, used for injecting gas into the backwater pipe to create a negative pressure environment, drive the culture solution to flow into the backwater pipe and then flow back into the culture container; the bottom of the culture container is provided with a connecting pipe, a switch valve is arranged on the connecting pipe, the other end of the connecting pipe is connected with a nutrient circulation system, the nutrient circulation system is connected with the top of the culture container.

2. The pollution-free continuous propagation system for Parachlorella sp. according to claim 1, characterized in that: A heating pipe and a temperature control device are further included, one end of the heating pipe extends into the culture container, the other end is connected with the temperature control device outside the culture container.

3. The pollution-free continuous propagation system for Parachlorella sp. according to claim 1, characterized in that: The nutrient circulation system includes a centrifugal filter, a transfer container and a sterilization device, the culture solution flowing out of the bottom of the culture container enters the centrifugal filter, the obtained paraflocculina is collected and used, the liquid after centrifugation enters the transfer container and then enters the sterilization device for sterilization and then returns to the culture container.

4. The pollution-free continuous propagation system for Parachlorella sp. according to claim 1, characterized in that: The gas supply system includes a gas pump, an air inlet pipe and a gas device gas supply outer cavity, the two ends of the air inlet pipe are respectively connected with the gas pump and the gas device gas supply outer cavity, the gas device gas supply outer cavity is fixed at the bottom of the backwater pipe and is in sealed connection with the backwater pipe, a plurality of air inlet holes are arranged on the backwater pipe, the gas output from the gas pump enters the air inlet pipe, the gas device gas supply outer cavity and then the backwater pipe through the air inlet holes.

5. The pollution-free continuous propagation system for Parachlorella sp. according to claim 1, characterized in that: The top of the culture container is provided with a sealing cover, the cover is transparent and made of rigid glass or plastic film material.

6. The pollution-free continuous propagation system for Parachlorella sp. according to claim 5, characterized in that: A plurality of exhaust holes are arranged on the cover.

7. The pollution-free continuous propagation system for Parachlorella sp. according to claim 1, characterized in that: The main body of the culture container is made of plastic material and has a conical shape.

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