Cell hypoxia culture bottle
By designing a cell hypoxia culture flask, utilizing a circulation channel and deoxygenation cylinder to reduce oxygen concentration, and combining it with oxygen sensors and heating plate control, the problems of high cost and large size of existing equipment are solved, achieving low-cost and convenient hypoxia culture results.
Patent Information
- Application Number
- CN202422831570.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-20
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2034-11-20
AI Technical Summary
Existing cell hypoxia culture equipment is expensive and bulky, making it wasteful to use large equipment when conducting small-scale cell hypoxia culture.
A cell hypoxia culture flask was designed, which includes components such as a circulation channel, a deoxygenation cylinder, an oxygen sensor, and a heating plate. The oxygen concentration is reduced by the deoxygenation pack and circulation fan in the circulation channel, and the hypoxia environment is simulated by the oxygen sensor and touch screen control.
It achieves low-oxygen culture with simple structure and convenient operation, reduces costs, is suitable for small-scale cell culture, and avoids the waste of large-scale equipment.
Smart Images
Figure CN223522564U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell hypoxic culture technical field, concretely is a cell hypoxic culture bottle. BACKGROUND
[0002] Cell hypoxic culture is an experimental technique that simulates the hypoxic environment in the body by adjusting the oxygen concentration in the cell culture conditions to study the biological characteristics and physiological functions of cells under hypoxic conditions. Under normal cell culture conditions, the oxygen concentration is usually 20%, while in some tissues or pathological states in the body, the oxygen concentration may be reduced. In cancer research, hypoxic environment is often related to tumor growth, progression and treatment resistance. By simulating the hypoxic environment, the survival mechanism and metabolic adaptation of tumor cells under hypoxic conditions can be better understood. In addition, cell hypoxic culture conditions also have important significance in the research field of cardiovascular diseases, neurodegenerative diseases, etc. These diseases are often related to hypoxic environment in the development process.
[0003] When performing cell hypoxic culture experiments, CO2 incubator, hypoxic chamber, high-pressure steel cylinder containing different concentrations of oxygen, hypoxic indicator, well-grown cell lines and various basic experimental tools need to be prepared. Cells are divided into different culture dishes and cultured with 1x growth medium and 10% FBS respectively. The high-pressure steel cylinder containing a specific oxygen concentration is connected to the hypoxic chamber, and the hypoxic indicator is used to detect the degree of hypoxia. When the desired hypoxic level is reached in the chamber, the cell culture dishes are placed in the hypoxic chamber for culture. After completing the hypoxic culture, the cell samples are taken out and subjected to subsequent experimental analysis.
[0004] However, the common cell hypoxic culture equipment has high cost and large volume, and it is wasteful to use large equipment when a small amount of cell hypoxic culture is performed. Therefore, the present application provides a cell hypoxic culture bottle. UTILITY MODEL CONTENT
[0005] In view of the shortcomings of the prior art, the utility model provides a cell hypoxic culture bottle, which has the advantages of simple structure, low culture cost, etc. and solves the problem of high cost and large volume of common cell hypoxic culture equipment, which is wasteful when a small amount of cell hypoxic culture is performed.
[0006] To achieve the above purpose, the utility model provides the following technical scheme: a cell hypoxic culture bottle, comprising a culture bottle, a circulation channel is arranged on the bottle body of the culture bottle, a deoxygenation cylinder is arranged on the circulation channel, a mounting mesh plate is fixedly installed on the inner side wall of the deoxygenation cylinder, a circulation fan is fixedly installed on one side of the mounting mesh plate, and a deoxygenation material bag is placed in the deoxygenation cylinder.
[0007] Further, one side of the culture bottle is fixedly installed with a circulating out pipe, and one side of the culture bottle is fixedly installed with a circulating in pipe.
[0008] Further, the circulating channel comprises a first hose fixedly installed at one end of the circulating out pipe, and a second hose fixedly installed at one end of the circulating in pipe.
[0009] Further, the deoxygenation cylinder is fixedly installed at one end of the first hose, and the second hose is rotatably installed with a docking cover which is threadedly connected with the deoxygenation cylinder.
[0010] Further, the bottom of the bottle mouth of the culture bottle is fixedly installed with a limiting ring, and the top of the bottle mouth of the culture bottle is threadedly installed with a sealing cover.
[0011] Further, the inner side wall of the culture bottle is fixedly installed with an oxygen sensor, and the bottle body of the culture bottle is fixedly installed with a touch screen.
[0012] Further, the bottom of the culture bottle is fixedly installed with a control base, the oxygen sensor is electrically connected with the control base, and the touch screen is electrically connected with the control base.
[0013] Further, the inner bottom wall of the culture bottle is fixedly installed with a heating plate, and the heating plate is electrically connected with the control base.
[0014] Compared with the prior art, the cell low-oxygen culture bottle has the following beneficial effects:
[0015] The cell low-oxygen culture bottle has the following beneficial effects: the circulating channel composed of the first hose, the deoxygenation cylinder, the second hose and the docking cover is arranged between the circulating out pipe and the circulating in pipe on the bottle body of the culture bottle, the deoxygenation material bag containing the deoxidizing agent is arranged in the deoxygenation cylinder, the air in the culture bottle is driven to flow by the circulating fan in the deoxygenation cylinder, and the oxygen content in the air is reduced, so that the structure is simple, the operation is convenient, and the cell low-oxygen culture is suitable for a small amount of cells, and the problem that the common cell low-oxygen culture equipment has high cost and large size and is wasted when a small amount of cells is cultured by using a large equipment is solved. BRIEF DESCRIPTION OF DRAWINGS
[0016] Fig. 1 It is a structural schematic view of the utility model;
[0017] Fig. 2 It is a structural front view of the utility model;
[0018] Fig. 3 It is a front view of the deoxygenation cylinder of the utility model;
[0019] Fig. 4 It is a three-dimensional structural schematic view of the utility model.
[0020] In the figure: 1, culture bottle; 2, circulating out pipe; 3, first hose; 4, deoxygenation cylinder; 5, circulating in pipe; 6, second hose; 7, docking cover; 8, installation mesh plate; 9, circulating fan; 10, deoxygenation material package; 11, limiting ring; 12, sealing cover; 13, oxygen sensor; 14, touch screen; 15, control base; 16, heating plate. DETAILED DESCRIPTION
[0021] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0022] Please refer to Figs. 1 to 4 A cell low oxygen culture bottle, comprising a culture bottle 1, a circulating channel is arranged on the bottle body of the culture bottle 1, a deoxygenation cylinder 4 is arranged on the circulating channel, an installation mesh plate 8 is fixedly installed on the inner side wall of the deoxygenation cylinder 4, a circulating fan 9 is fixedly installed on one side of the installation mesh plate 8, and a deoxygenation material package 10 is placed in the deoxygenation cylinder 4. The deoxygenation material package 10 containing an oxygen scavenger is placed in the deoxygenation cylinder 4, and the circulating fan 9 drives the air in the culture bottle 1 to flow in the circulating channel, so that the oxygen content in the culture bottle 1 is reduced, and a low oxygen environment is formed.
[0023] The culture bottle 1 is fixedly installed with a circulating out pipe 2 on one side, and the culture bottle 1 is fixedly installed with a circulating in pipe 5 on one side.
[0024] Secondly, the circulating channel comprises a first hose 3 fixedly installed at one end of the circulating out pipe 2, and a second hose 6 fixedly installed at one end of the circulating in pipe 5.
[0025] The deoxygenation cylinder 4 is fixedly installed at one end of the first hose 3, and a docking cover 7 is rotatably installed at one end of the second hose 6 and is in threaded connection with the deoxygenation cylinder 4. The circulating fan 9 is started, the air in the culture bottle 1 enters the second hose 6 through the circulating in pipe 5, enters the deoxygenation cylinder 4 through the docking cover 7, enters the first hose 3 through the deoxygenation cylinder 4, enters the circulating out pipe 2 through the first hose 3, and flows back into the culture bottle 1 through the circulating out pipe 2.
[0026] After the deoxygenation material package 10 is installed in the deoxygenation cylinder 4, the opening of the deoxygenation cylinder 4 is communicated with the second hose 6 through the docking cover 7, so that the circulating channel is closed. When the deoxygenation material package 10 needs to be replaced, the docking cover 7 is unscrewed from one end of the deoxygenation cylinder 4, the deoxygenation material package 10 is taken out, a new deoxygenation material package 10 is placed in the deoxygenation cylinder 4, and then the docking cover 7 is installed, so that the replacement of the deoxygenation material package 10 is completed.
[0027] Meanwhile, the bottom of the bottle mouth of the culture bottle 1 is fixedly installed with a limiting ring 11, and the top of the bottle mouth of the culture bottle 1 is threadedly installed with a sealing cover 12. The cells and the culture solution are loaded into the culture bottle 1 from the bottle mouth of the culture bottle 1, and the culture bottle 1 is sealed by the sealing cover 12.
[0028] The inner side wall of the culture bottle 1 is fixedly installed with an oxygen sensor 13, and the bottle body of the culture bottle 1 is fixedly installed with a touch screen 14.
[0029] Secondly, the bottom of the culture bottle 1 is fixedly installed with a control base 15, the oxygen sensor 13 is electrically connected with the control base 15, and the touch screen 14 is electrically connected with the control base 15. The oxygen concentration in the culture bottle 1 is detected by the oxygen sensor 13 and is converted into an electric signal and transmitted to the control base 15, the required oxygen concentration is input through the touch screen 14, and the processor in the control base 15 is closed when the oxygen concentration reaches the specified value. The circulating fan 9, and the real-time oxygen concentration in the culture bottle 1 is displayed on the touch screen 14.
[0030] Finally, the inner bottom wall of the culture bottle 1 is fixedly installed with a heating plate 16, and the heating plate 16 is electrically connected with the control base 15. When it is needed to improve the culture temperature, the culture solution is heated by the heating plate 16 to improve the temperature of the culture solution.
[0031] In use, the cells and the culture solution are loaded into the culture bottle 1 from the bottle mouth of the culture bottle 1, the culture bottle 1 is sealed by the sealing cover 12, the required oxygen concentration is set through the touch screen 14, the control base 15 starts the circulating fan 9, and the air in the culture bottle 1 is circulated in the circulating channel, the oxygen in the air is absorbed by the deoxidizing agent in the deoxidizing material bag 10, the oxygen concentration in the culture bottle 1 is reduced, and the control base 15 is closed when the oxygen concentration reaches the specified value. The circulating fan 9 is closed, so that the culture bottle 1 is in a low-oxygen state, and the cell hypoxia culture is facilitated.
[0032] The electrical components appearing in the text are electrically connected with the main controller and the power supply, the main controller can be a conventional known device such as a computer for control, and the existing disclosed power connection technology is not described herein.
[0033] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting; it is not intended to exclude myriad other embodiments of the present application that other inventors can develop based on the same general inventive concepts embodied by the described embodiments. That is, although the present application is described in terms of particular embodiments and implementations, it is to be understood that the terminology used is for the purpose of descriptive clarity and that it is intended to be limited only by the words recited in the appended claims. The scope of the present application shall be limited only by the claims.
[0034] While the embodiments of the present application have been shown and described with respect to particular embodiments thereof, it will be understood by those skilled in the art that various changes in form and details can be made therein without departing from the spirit and scope of the application. Therefore, the scope of the application should not be limited by the embodiments, but should be defined only in accordance with the following claims and their equivalents.
Claims
1. A cell hypoxic flask comprising a flask (1), characterised in that: The culture bottle (1) is provided with a circulating channel on the bottle body, a deoxygenation cylinder (4) is arranged on the circulating channel, an installation net plate (8) is fixedly installed on the inner side wall of the deoxygenation cylinder (4), a circulating fan (9) is fixedly installed on one side of the installation net plate (8), and a deoxygenation material bag (10) is placed in the deoxygenation cylinder (4).
2. The cell hypoxic flask of claim 1, wherein: One side of the culture bottle (1) is fixedly provided with a circulating outlet pipe (2), and one side of the culture bottle (1) is fixedly provided with a circulating inlet pipe (5).
3. The cell hypoxic flask of claim 2, wherein: The circulating channel comprises a first hose (3) fixedly installed at one end of the circulating outlet pipe (2), and a second hose (6) fixedly installed at one end of the circulating inlet pipe (5).
4. The cell hypoxic flask of claim 3, wherein: The deoxygenation cylinder (4) is fixedly installed at one end of the first hose (3), and a butt joint cover (7) is rotatably installed at one end of the second hose (6), and the butt joint cover (7) is threadedly connected with the deoxygenation cylinder (4).
5. The cell hypoxic flask of claim 1, wherein: A limiting ring (11) is fixedly installed at the bottom of the bottle opening of the culture bottle (1), and a sealing cover (12) is threadedly installed at the top of the bottle opening of the culture bottle (1).
6. The cell hypoxic flask of claim 1, wherein: An oxygen sensor (13) is fixedly installed on the inner side wall of the culture bottle (1), and a touch screen (14) is fixedly installed on the bottle body of the culture bottle (1).
7. The cell hypoxic flask of claim 6, wherein: A control base (15) is fixedly installed at the bottom of the culture bottle (1), the oxygen sensor (13) is electrically connected with the control base (15), and the touch screen (14) is electrically connected with the control base (15).
8. The cell hypoxic flask of claim 7, wherein: A heating plate (16) is fixedly installed on the inner bottom wall of the culture bottle (1), and the heating plate (16) is electrically connected with the control base (15).