Liquid strain culture device with automatic oxygen supply function

Abstract

The embodiment of the utility model provides a liquid strain culture device with an automatic oxygen supply function, which relates to the technical field of liquid strain culture and comprises a culture barrel, an air inlet pipe is arranged at the upper end of the culture barrel, one end of the air inlet pipe is inserted into the culture barrel, and the other end of the air inlet pipe is connected with an air compressor. The air inlet pipe and the air outlet pipe are mounted at the top of the upper end of the rotary cover, the air compressor is started to ensure that the culture solution in the culture barrel can be stirred normally, and the whole set of device is placed in a culture room or an incubator for temperature-controlled culture until a large number of fungus balls are formed; after the culture solution becomes transparent, culture is terminated, the culture solution is inoculated into a culture bag, sterile gas is introduced into the nutrient solution in a culture barrel under the action of an air compressor, the purposes of aeration, oxygenation, uniform agitation and rapid culture of liquid strains are achieved, and the production and inoculation efficiency of the edible fungus strains can be greatly improved in cooperation with liquid inoculation equipment.

Description

Technical Field

[0001] This utility model relates to the field of liquid microbial culture technology, and in particular to a liquid microbial culture device with automatic oxygen supply. Background Technology

[0002] Edible mushroom spawn is mycelium cultured in a suitable culture medium. It serves as the "seed" in edible mushroom production, and its quality directly impacts the success and profitability of the production. Edible mushroom spawn is classified into solid and liquid spawn based on its physical properties. Liquid spawn offers significant advantages over solid spawn, including shorter production cycles, easier inoculation, faster mycelial growth, more consistent mycelial age, and greater ease of mechanization, representing the future trend in edible mushroom spawn cultivation.

[0003] One of the main challenges in liquid culture is the oxygen supply in the culture environment. Sufficient oxygen is crucial for microbial growth, especially under high-density culture conditions, where oxygen deficiency significantly limits the growth rate and quality of mycelium. Traditional liquid culture systems often increase dissolved oxygen in the culture medium through simple aeration or stirring, but these two methods are inefficient and make it difficult to effectively control the culture environment. Utility Model Content

[0004] The purpose of this invention is to provide a liquid microbial culture device with automatic oxygen supply, which can avoid the problem that oxygen deficiency will significantly limit the growth rate and quality of mycelium.

[0005] This utility model provides a liquid bacterial culture device with automatic oxygen supply, comprising:

[0006] A culture tank is provided with an air inlet pipe at the upper end. One end of the air inlet pipe is inserted into the inside of the culture tank and the other end is connected to an air compressor. An air outlet pipe is also provided at the upper end of the culture tank. The top of the culture tank is opened and sealed by a rotating cover. Both the air inlet pipe and the air outlet pipe are installed on the top of the rotating cover.

[0007] Preferably, a pipe connector is also installed on the upper end of the rotating cover, the air inlet pipe and the air outlet pipe are installed on the upper end of the pipe connector, and an air blower pipe is connected to the lower end of the air inlet pipe, which is installed at the lower end of the pipe connector.

[0008] Preferably, a disc filter is installed at the upper end of both the air inlet pipe and the air outlet pipe, and both ends of the disc filter are connected by a tube body.

[0009] Preferably, a sealing part is movably installed at the upper end of the rotating cover. The sealing part includes a rotating frame for rotating and opening / closing and a telescopic cylinder. The telescopic cylinder is movably supported by a first mounting head movably installed on one side. The lower end of the rotating frame is supported and fixed by a second mounting head. A cover plate mounting head is movably installed at the end of the rotating frame.

[0010] Preferably, the culture tank is provided in two sets. The culture tank is supported and installed by a support frame provided on one side. Mounting feet are fixedly installed on both sides of the support frame. Hanging feet are fixedly installed on the sides of the culture tank. The mounting feet are inserted into the hanging feet for installation.

[0011] Preferably, the cover plate mounting head includes a connector installed at the end of the rotating frame, a fixing plate is fixedly installed at the lower end of the connector, a connecting ring is movably installed at the lower end of the fixing plate, and the connecting ring is threadedly connected to a threaded disc fixedly installed at the upper end of the rotating cover.

[0012] Preferably, a distribution pipe is installed at the end of the air blower, and the upper end of the distribution pipe is uniformly provided with slots.

[0013] Preferably, a positioning suction cup is fixedly installed at the lower end of the distribution tube, and the positioning suction cup is adsorbed and installed in the inner cavity of the culture tank.

[0014] Preferably, the upper end of the intake pipe may be fitted with an adjustment part, the adjustment part including a first mounting plate and a second mounting plate, both sides of the first mounting plate and the second mounting plate are movably mounted with movable rods, the upper end of the movable rod is fixedly mounted with a fixed plate, the middle part of the second mounting plate is movably mounted with a rotating screw, the lower end of the rotating screw is movably mounted through a bearing, the rotating screw is mounted through a screw hole opened in the middle of the fixed plate and is threadedly mounted with the screw hole in the middle of the fixed plate.

[0015] Preferably, a hook is fixedly inserted into the upper end of the support frame, and the lower end of the hook is inserted into the upper end of the support frame through a connecting seat and fixed by screws.

[0016] This utility model provides a liquid microbial culture device with automatic oxygen supply. After assembling the device, check its sealing integrity, open the rotating lid, add the weighed nutrients to the culture tank, add water and stir well, close the rotating lid, and insert filter cotton into the air inlet and outlet pipes. Place the device in an autoclave at 121°C for 30 minutes for sterilization. After sterilization, allow it to cool naturally, remove the device and place it on a clean bench. Irradiate with ultraviolet light for 30 minutes, open the rotating lid, quickly pour in the liquid microbial culture from the shake flask, and quickly close the rotating lid. After the inoculation is complete, connect the air outlet of the air compressor to the air inlet of the filter cotton. Start the air compressor to ensure that the culture medium in the culture tank can be agitated normally. Place the entire device in a culture room or incubator for temperature-controlled inoculation. Stop the inoculation when a large number of mycelial balls have formed and the culture medium becomes transparent. Inoculate the culture bag. Under the action of the air compressor, sterile gas is introduced into the nutrient solution in the culture tank to achieve the purpose of ventilation, oxygenation, uniform agitation, and rapid cultivation of liquid mycelium. Combined with liquid inoculation equipment, it can greatly improve the production and inoculation efficiency of edible fungi spawn. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model.

[0019] Figure 2 This is a schematic diagram of the culture tank structure according to an embodiment of the present invention.

[0020] Figure 3 This is a schematic diagram of the support frame structure according to an embodiment of the present utility model.

[0021] Figure 4 This is a schematic diagram of the rotating cover structure according to an embodiment of the present utility model.

[0022] Figure 5 This is a schematic diagram of the distribution pipe structure according to an embodiment of the present utility model.

[0023] Figure 6 This is an embodiment of the present utility model. Figure 3 Enlarged structural diagram at point A in the middle.

[0024] Figure 7 This is an embodiment of the present utility model. Figure 2 Enlarged structural diagram at point B.

[0025] Figure 8 This is an embodiment of the present utility model. Figure 3 Enlarged structural diagram at point C.

[0026] Figure Descriptions: 100, Culture Tank; 110, Air Inlet Pipe; 111, Air Blower Pipe; 120, Air Compressor; 130, Disc Filter; 140, Air Outlet Pipe; 150, Hanging Foot; 160, Distribution Pipe; 161, Positioning Suction Cup; 200, Support Frame; 210, Mounting Foot; 220, Second Mounting Head; 230, First Mounting Head; 240, Connecting Seat; 250, Hook; 300, Sealing Part; 310, Rotating Frame; 320, Telescopic Cylinder; 330, Cover Plate Mounting Head; 331, Connecting Head; 332, Fixing Plate; 333, Connecting Ring; 400, Adjustment Part; 410, First Mounting Plate; 420, Movable Rod; 430, Second Mounting Plate; 440, Rotating Screw; 450, Fixing Plate; 500, Rotating Cover; 510, Threaded Disc; 520, Pipe Connector. Detailed Implementation

[0027] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0028] In the description of the embodiments of this utility model, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model.

[0029] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0030] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0031] The following disclosure provides many different implementations or examples for different structures of the embodiments of the present invention. To simplify the disclosure of the embodiments of the present invention, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the embodiments of the present invention. Furthermore, reference numerals and / or reference letters may be repeated in different examples of the embodiments of the present invention; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various implementations and / or arrangements discussed.

[0032] To better understand the purpose, structure, and function of this utility model, the following detailed description of a liquid microbial culture device with automatic oxygen supply, in conjunction with the accompanying drawings, is provided.

[0033] like Figures 1-8 As shown, this utility model embodiment provides a liquid microbial culture device with automatic oxygen supply, including a culture tank 100 for culturing and propagating liquid microorganisms. The upper end of the culture tank 100 is provided with an air inlet pipe 110 for inflating the inside of the culture tank 100. One end of the air inlet pipe 110 is inserted into the inside of the culture tank 100, and the other end of the air inlet pipe 110 is connected to an air compressor 120. The upper end of the culture tank 100 is also provided with an air outlet pipe 140 for discharging the air inside the culture tank 100. The top of the culture tank 100 is opened and closed and sealed by a rotating cover 500. A sealing ring is arranged on the upper end of the culture tank 100 for auxiliary sealing, which is matched with the rotating cover 500 for closure. The air inlet pipe 110 and the air outlet pipe 140 are both installed on the top of the rotating cover 500.

[0034] After assembling the apparatus, check and confirm its sealing integrity. Open the rotating lid 500, add the weighed nutrients to the culture tank 100, add water and stir well. Close the rotating lid 500, and insert filter cotton into the inlet pipe 110 and outlet pipe 140. Place it in an autoclave at 121℃ for 30 minutes for sterilization. After sterilization, allow it to cool naturally, remove the apparatus and place it on a clean bench. Irradiate with ultraviolet light for 30 minutes. Open the rotating lid 500, quickly pour in the liquid culture from the shake flask, and quickly close the rotating lid 500. After inoculation, turn on the air compressor 1. Connect the outlet pipe 140 of the 20 to the inlet pipe 110 for removing the filter cotton. Start the air compressor 120 to ensure that the culture solution in the culture tank 100 can be agitated normally. Place the entire device in a culture room or incubator for temperature-controlled culture. Stop the culture when a large number of mycelial balls have formed and the culture solution becomes transparent. Inoculate the culture bag. Under the action of the air compressor 120, sterile gas is introduced into the nutrient solution in the culture tank 100 to achieve the purpose of ventilation, oxygenation, uniform agitation, and rapid culture of liquid mycelium. Combined with liquid inoculation equipment, it can greatly improve the production and inoculation efficiency of edible fungi spawn.

[0035] The upper end of the rotating cover 500 is also equipped with a pipe connector 520 for installing the air inlet pipe 110 and the air outlet pipe 140. The pipe connector 520 can be a through-plate pagoda type connector. The air inlet pipe 110 and the air outlet pipe 140 are installed on the upper end of the pipe connector 520. The lower end of the air inlet pipe 110 is connected to an air blowing pipe 111 for probing into the liquid. The air blowing pipe 111 is installed on the lower end of the pipe connector 520. The through-plate pagoda type pipe connector 520 can connect the pipes on both sides and seal them when not in use to ensure the culture environment of the culture tank 100.

[0036] Both the upper ends of the air inlet pipe 110 and the air outlet pipe 140 are equipped with disc filters 130 for filtering the incoming and outgoing air. Both ends of the disc filters 130 are installed by inserting them into the pipe body, which can filter the air entering and leaving the culture tank 100 to ensure that the incoming and outgoing air is clean.

[0037] A sealing part 300 for rotating and opening the rotating cover 500 is movably mounted on the upper end of the rotating cover 500. The sealing part 300 includes a rotating frame 310 for rotating and opening, and a telescopic cylinder 320 for rotating the rotating frame 310. The telescopic cylinder 320 is movably supported by a first mounting head 230 movably mounted on one side. The lower end of the rotating frame 310 is supported and fixed by a second mounting head 220. The telescopic rod end of the telescopic cylinder 320 is movably mounted on the end of the rotating frame 310 via a rotating shaft. A sealing part for rotating and opening the rotating cover 500 is movably mounted on the end of the rotating frame 310. The rotating cover 500 has a rotating cover mounting head 330. When the rotating cover 500 is opened and closed, the telescopic cylinder 320 drives the telescopic rod to retract. The rotating frame 310 installed at the upper end drives the rotating cover 500 to rotate and open and close. This allows for easy adjustment of the extension length of the telescopic cylinder 320 to control the sealing degree of the rotating cover 500, and eliminates the need for manual opening and closing, thus avoiding contamination. If it is necessary to sterilize the culture tank 100, the cover mounting head 330 can be rotated off and the culture tank 100 can be removed for sterilization. During disassembly, the rotating cover 500 locks shut due to its own weight, preventing leakage.

[0038] The culture tank 100 is provided in two sets. The culture tank 100 is supported and installed by a support frame 200 on one side. Mounting feet 210 for installing the culture tank 100 are fixedly installed on both sides of the support frame 200. Hanging feet 150 for installing and supporting the culture tank 100 are fixedly installed on the sides of the culture tank 100. The mounting feet 210 are inserted into the hanging feet 150 for installation. The mounting feet 210 and hanging feet 150 facilitate the placement and positioning of the culture tank 100, and the installation and disassembly are convenient.

[0039] The cover plate mounting head 330 includes a connector 331 installed at the end of the rotating frame 310 for connection. A fixed plate 332 for connection support is fixedly installed at the lower end of the connector 331. A connecting ring 333 for rotation with the rotating frame 310 is movably installed at the lower end of the fixed plate 332. The connecting ring 333 is threadedly connected to a threaded plate 510 fixedly installed at the upper end of the rotating cover 500. When it is necessary to separate the cover plate mounting head 330 from the culture tank 100, the connecting ring 333 can be rotated off from the upper end of the threaded plate 510 for easy installation and disassembly.

[0040] The end of the air-blowing pipe 111 is equipped with a distribution pipe 160 for introducing air. The upper end of the distribution pipe 160 is evenly provided with slots for air blowing, which can evenly distribute the injected air in the inner cavity of the culture tank 100, so that the ventilation and oxygenation are uniform and the blowing effect is better.

[0041] A positioning suction cup 161 for fixing the distribution tube 160 is fixedly installed at the lower end of the distribution tube 160. The positioning suction cup 161 is attached to the inner cavity of the culture tank 100 to prevent the distribution tube 160 from shaking and shifting during aeration, thus affecting the aeration effect.

[0042] An adjustment part 400 for controlling and adjusting the internal air intake volume can be fitted onto the upper end of the intake pipe 110. The adjustment part 400 includes a first mounting plate 410 and a second mounting plate 430 for clamping and supporting the intake pipe 110. Movable rods 420 for supporting and installing the first mounting plate 410 and the second mounting plate 430 are movably mounted on both sides of the first mounting plate 410 and the second mounting plate 430. A fixing plate 450 for connecting and supporting the movable rod 420 is fixedly mounted on the upper end of the movable rod 420. A fixing plate 450 for connecting and supporting the movable rod 420 is movably mounted on the middle of the second mounting plate 430. The rotating screw 440 is supported by the second mounting plate 430. The lower end of the rotating screw 440 is movably mounted via a bearing. The rotating screw 440 is installed through a screw hole in the middle of the fixed plate 450 and is threaded into the screw hole in the middle of the fixed plate 450. When aeration is provided, the rotating screw 440 can be rotated, thereby pushing the second mounting plate 430 on one side to move. The air inlet pipe 110 in the middle is clamped by the first mounting plate 410 and the second mounting plate 430. The air intake volume is adjusted by adjusting the position and tightness of the rotating screw 440 to ensure that the culture medium in the culture tank 100 can be agitated normally.

[0043] The upper end of the support frame 200 is fixedly fitted with a hook 250 for lifting and transporting the support frame 200. The lower end of the hook 250 is inserted into the upper end of the support frame 200 through a connecting seat 240 and fixed with screws. It can be connected to the hook 250 with tools to lift the entire equipment for easy transport.

[0044] The working principle of a liquid microbial culture device with automatic oxygen supply is as follows: Open the rotating cap 500, quickly pour in the liquid microbial culture from the shake flask, and quickly close the rotating cap 500. After inoculation, connect the air outlet 140 of the air compressor 120 to the air inlet 110 from which the filter cotton is removed. Start the air compressor 120 to ensure that the culture solution in the culture tank 100 can be agitated normally. Place the entire device in a culture room or incubator for temperature-controlled inoculation. After a large number of mycelial balls have formed and the culture solution has become transparent, stop the inoculation and inoculate into the cultivation bag. Under the action of the air compressor 120, sterile gas is introduced into the nutrient solution in the culture tank 100 to achieve the purpose of ventilation and oxygenation, uniform agitation, and rapid cultivation of liquid microbial culture. Combined with liquid inoculation equipment, it can greatly improve the production and inoculation efficiency of edible fungi spawn.

[0045] It is understood that this utility model has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of this utility model. Furthermore, under the teachings of this utility model, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of this utility model.

Claims

1. A liquid microbial culture device with automatic oxygen supply, characterized in that, include: A culture tank is provided with an air inlet pipe at the upper end. One end of the air inlet pipe is inserted into the inside of the culture tank and the other end is connected to an air compressor. An air outlet pipe is also provided at the upper end of the culture tank. The top of the culture tank is opened and sealed by a rotating cover. Both the air inlet pipe and the air outlet pipe are installed on the top of the rotating cover.

2. The liquid microbial culture device with automatic oxygen supply according to claim 1, characterized in that, The upper end of the rotating cover is also equipped with a pipe connector. The air inlet pipe and the air outlet pipe are installed at the upper end of the pipe connector. The lower end of the air inlet pipe is connected to a blower pipe, which is installed at the lower end of the pipe connector.

3. The liquid microbial culture device with automatic oxygen supply according to claim 2, characterized in that, Both the upper ends of the air inlet pipe and the air outlet pipe are equipped with disc filters, and both ends of the disc filters are installed by inserting them into the pipe body.

4. The liquid microbial culture device with automatic oxygen supply according to claim 3, characterized in that, The upper end of the rotating cover is movably fitted with a sealing part, which includes a rotating frame for rotating and opening / closing and a telescopic cylinder. The telescopic cylinder is movably supported by a first mounting head movably mounted on one side. The lower end of the rotating frame is supported and fixed by a second mounting head. A cover plate mounting head is movably mounted at the end of the rotating frame.

5. A liquid microbial culture device with automatic oxygen supply according to claim 4, characterized in that, The culture tank is provided in two sets. The culture tank is supported and installed by a support frame provided on one side. Mounting feet are fixedly installed on both sides of the support frame. Hanging feet are fixedly installed on the sides of the culture tank. The mounting feet are inserted into the hanging feet for installation.

6. A liquid microbial culture device with automatic oxygen supply according to claim 5, characterized in that, The cover plate mounting head includes a connector installed at the end of the rotating frame. A fixing plate is fixedly installed at the lower end of the connector, and a connecting ring is movably installed at the lower end of the fixing plate. The connecting ring is threadedly connected to a threaded plate fixedly installed at the upper end of the rotating cover.

7. A liquid microbial culture device with automatic oxygen supply according to claim 6, characterized in that, A distribution pipe is installed at the end of the air blower, and the upper end of the distribution pipe is evenly provided with slots.

8. A liquid microbial culture device with automatic oxygen supply according to claim 7, characterized in that, A positioning suction cup is fixedly installed at the lower end of the distribution tube, and the positioning suction cup is adsorbed and installed in the inner cavity of the culture tank.

9. A liquid microbial culture device with automatic oxygen supply according to claim 8, characterized in that, An adjustment part can be fitted onto the upper end of the air intake pipe. The adjustment part includes a first mounting plate and a second mounting plate. Movable rods are movably mounted on both sides of the first and second mounting plates. A fixed plate is fixedly mounted on the upper end of the movable rod. A rotating screw is movably mounted in the middle of the second mounting plate. The lower end of the rotating screw is movably mounted via a bearing. The rotating screw passes through a screw hole opened in the middle of the fixed plate and is threadedly mounted to the screw hole in the middle of the fixed plate.

10. A liquid microbial culture device with automatic oxygen supply according to claim 9, characterized in that, The upper end of the support frame is fixedly fitted with a hook, and the lower end of the hook is inserted into the upper end of the support frame through a connecting seat and fixed with screws.

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