Agar gel negative pressure pore-forming device

Abstract

The utility model relates to the technical field of agar gel punching, in particular to an agar gel negative pressure hole forming device which comprises a hole forming device body connected with negative pressure equipment, and the hole forming device body comprises a material storage barrel and an installation barrel. An opening is formed between the opposite ends of the storage barrel and the mounting barrel, and the storage barrel and the mounting barrel are matched for use through a connecting part at the opening; wherein the top of the storage barrel communicates with a connector, a filter screen is arranged at the position, located at an opening of the connector, in the storage barrel, and a plurality of punching pipes are detachably connected to the bottom of the mounting barrel through fixing pieces, so that quick disassembly and assembly can be conducted, and the filter screen can be conveniently disassembled to clean broken gel blocks; and the punching pipe can be independently replaced through the fixing piece, the situation that normal punching of the gel block is affected due to the fact that the punching pipe is tedious to replace after long-time use is avoided, and in addition, the situation that waste is caused due to the fact that a plurality of punching pipes need to be replaced at the same time can be avoided.

Description

Technical Field

[0001] This utility model relates to the field of agar gel perforation technology, and in particular to a perforator for agar gel negative pressure perforation. Background Technology

[0002] Agarose gel is a gel prepared with agarose as the main component. Agarose is a neutral polysaccharide extracted from agar and is mainly composed of galactose and its derivatives. This type of gel is often used for in situ enzymatic digestion of chromosomal DNA and recovery of DNA fragments within the gel. Due to its large pore size, it is often used for the separation of large protein molecules and DNA.

[0003] When preparing agarose gel for electrophoresis, it is necessary to perforate the gel so that the sample can be placed inside for separation under an electric field. For example, Chinese Patent Publication No. CN106881747A discloses a method and apparatus for creating pores in agarose gel under negative pressure, specifically involving a one-step, highly efficient method and apparatus for perforation and negative pressure adsorption. To overcome the problems of the prior art, the problem to be solved by this invention is: a method for creating pores in agarose gel under negative pressure, comprising the following sequential steps: a. alignment step, b. insertion and perforation step, c. vacuuming, d. continuous insertion and perforation step, e. negative pressure adsorption; the pore-forming apparatus consists of a material-holding body, one end of which is sealed to the tail of the perforator, and the other end of which is sealed to a vacuum system; the material-holding body is a cavity capable of holding gel particles; this invention can be widely used in the field of agarose gel perforation.

[0004] However, in actual operation, the fixed connection between the perforated tube and the material holding body is not easy to disassemble and assemble. After long-term use, the perforated tube will age and be damaged, making it inconvenient to replace. Or, multiple perforated tubes need to be replaced as a whole, which not only affects the normal perforation operation of the gel block, but also wastes resources. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies, such as the inconvenience of replacing the pore tube with the material holding body, and to propose a pore-forming device for agar gel negative pressure pore forming.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A pore-forming device for agarose gel negative pressure pore forming is designed, including a pore-forming device body connected to a negative pressure device. The pore-forming device body includes a storage cylinder and an installation cylinder. An opening is provided between the opposite ends of the storage cylinder and the installation cylinder, and they are used in conjunction with a connecting part at the opening.

[0008] The storage cylinder has a connector at the top and a filter screen inside the connector opening. The bottom of the mounting cylinder is detachably connected to several perforated tubes via fasteners.

[0009] Furthermore, the filter screen has a cylindrical structure, with an opening at one end that abuts against the inner side of the mounting cylinder, and a compression spring between the other end and the storage cylinder. The filter screen is used to hold broken gel blocks.

[0010] Furthermore, the fastener includes a connecting inner cylinder and a connecting outer cylinder. The connecting inner cylinder is fixedly connected to the bottom of the mounting cylinder and is threadedly connected to the connecting outer cylinder via internal and external threads. An elastic clamp is also provided inside the connecting inner cylinder.

[0011] Furthermore, the elastic gripper has a cylindrical structure, and its top periphery extends outward to form a protrusion. The top of the elastic gripper is provided with several deformation grooves at equal intervals along the axial direction, and the perforated tube is slidably connected inside it.

[0012] Furthermore, the top of the connecting outer cylinder is recessed to form an avoidance hole, and the inner wall of the avoidance hole extends downward to form an extension. The extension corresponds to the protrusion, and a guide slope is provided between their opposing ends. The connecting outer cylinder abuts against the protrusion through the extension so that the elastic gripper clamps the perforated tube.

[0013] Furthermore, a sealing ring is provided between one end of the perforated tube and the mounting cylinder.

[0014] The present invention provides a pore-forming device for agar gel under negative pressure, which has the following advantages: the device ensures good sealing between the installation cylinder and the storage cylinder through the connecting part, while also allowing for quick assembly and disassembly. This facilitates the removal of the filter screen for cleaning broken gel blocks. Furthermore, the fixing part allows for individual replacement of the pore-forming tube, preventing the cumbersome replacement of the pore-forming tube from affecting the normal pore-forming of the gel blocks after long-term use. In addition, it avoids the waste caused by having to replace multiple pore-forming tubes at the same time. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a cross-sectional view of the present invention;

[0017] Figure 3 This is a schematic diagram of the structure of the fastener of this utility model;

[0018] Figure 4 for Figure 3 A magnified structural diagram of area A.

[0019] In the diagram: 1. Main body of the perforator; 11. Storage cylinder; 12. Mounting cylinder; 13. Connector; 14. Compression spring; 2. Connecting part; 3. Filter screen; 4. Fixing component; 41. Connecting inner cylinder; 42. Connecting outer cylinder; 421. Extension part; 422. Guide slope; 43. Threaded line; 44. Elastic gripper; 441. Protrusion; 442. Deformation groove; 5. Perforation tube. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] Reference Figure 1-4 A pore-forming device for agarose gel negative pressure pore forming includes a pore-forming device body 1 connected to a negative pressure device. The pore-forming device body 1 includes a storage cylinder 11 and an installation cylinder 12. An opening is provided between the opposite ends of the storage cylinder 11 and the installation cylinder 12, and the cylinders are used in conjunction with a connecting part 2 at the opening.

[0022] The storage cylinder 11 has a connector 13 at its top and a filter screen 3 at the opening of the connector 13 inside. The bottom of the mounting cylinder 12 is detachably connected to several perforated tubes 5 via a fixing member 4.

[0023] It should be added that the negative pressure device is a vacuum pump or an air compressor. The negative pressure device is connected to an air guide pipe, which is connected to the connector 13 through a quick connector. Under the action of the negative pressure device, the main body 1 of the pore generator forms a negative pressure inside, which draws the broken gel block into the filter screen 3 through the perforation tube 5.

[0024] Specifically, the connecting part 2 includes an extension rod, which is integrally formed on the top of the mounting cylinder 12. The extension rod has a hollow structure and forms a step between it and the top of the mounting cylinder 12. The storage cylinder 11 is threadedly connected to the extension rod by internal and external threads. The bottom of the storage cylinder 11 is located around the extension rod and a sealing ring is provided between it and the step.

[0025] Furthermore, the filter screen 3 has a cylindrical structure. One end of the filter screen 3 has an opening that abuts against the inner side of the mounting cylinder 12, and the other end is provided with a compression spring 14 between it and the storage cylinder 11. The filter screen 3 is used to hold broken gel blocks.

[0026] It is worth mentioning that when the storage cylinder 11 is fastened to the mounting cylinder 12, the inner side of the mounting cylinder 12 abuts against the end of the filter screen 3 that has an opening, and the other end of the filter screen 3 is squeezed and then abuts against the compression spring 14. Under the elastic support of the compression spring 14, a fixing effect is achieved, and the broken gel blocks are collected.

[0027] One end of the compression spring 14 is fixedly connected to the inner side of the storage cylinder 11, and the other end of the compression spring 14 abuts against the filter screen 3. The compression spring 14 is located around the connector 13 to prevent blockage.

[0028] Furthermore, the fixing component 4 includes a connecting inner cylinder 41 and a connecting outer cylinder 42. The connecting inner cylinder 41 is fixedly connected to the bottom of the mounting cylinder 12 and is threadedly connected to the connecting outer cylinder 42 by internal and external threads 43. An elastic clamping claw 44 is also provided inside the connecting inner cylinder 41.

[0029] More specifically, the elastic gripper 44 has a cylindrical structure and its top periphery extends outward to form a protrusion 441. The top of the elastic gripper 44 is provided with a plurality of deformation grooves 442 at equal intervals along the axial direction, and the perforated tube 5 is slidably connected inside it.

[0030] In general, the top of the connecting outer cylinder 42 is recessed to form a clearance hole, and the inner wall of the clearance hole extends downward to form an extension 421. The extension 421 corresponds to the protrusion 441, and a guide slope 422 is provided between their opposing ends. The connecting outer cylinder 42 abuts against the protrusion 441 through the extension 421 so that the elastic gripper 44 clamps the perforated tube 5.

[0031] In specific operation, the elastic gripper 44 is made of plastic or rubber and is placed inside the connecting inner cylinder 41, matching the inner wall of the connecting inner cylinder 41, so that it can be replaced when it ages and is damaged.

[0032] Furthermore, when the connecting outer cylinder 42 is rotated to move downwards, the extension 421, in conjunction with the guide slope 422, abuts against the protrusion 441. The protrusion 441 is pressed against and, in conjunction with the deformation groove 442, causes the top of the elastic gripper 44 to contract, thereby clamping the perforated tube 5.

[0033] Of course, the elastic gripper 44 is sleeved around the perforated tube 5, and the diameter of its internal opening is larger than that of the perforated tube 5. The inner wall of the elastic gripper 44 is provided with an anti-slip pad to better clamp the perforated tube 5.

[0034] Finally, a sealing ring is provided between one end of the perforated tube 5 and the mounting cylinder 12.

[0035] Working method: During operation, the filter screen 3 is placed inside the storage cylinder 11 and the mounting cylinder 12, and then the storage cylinder 11 and the mounting cylinder 12 are fixed together by the connecting part 2;

[0036] Then, the perforated tube 5 is placed inside the elastic gripper 44. When the outer cylinder 42 is rotated to move it downward, the extension 421, in conjunction with the guide slope 422, abuts against the protrusion 441. The protrusion 441 is pressed against and, in conjunction with the deformation groove 442, causes the top of the elastic gripper 44 to contract, thereby clamping the perforated tube 5.

[0037] Finally, the air duct of the negative pressure device is connected to the connector 13. The negative pressure device creates a negative pressure inside the pore-forming body 1 to suck up the broken gel blocks, and the filter screen 3 is used to collect the gel blocks.

[0038] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A pore-forming device for agarose gel negative pressure pore forming, comprising a pore-forming device body (1) connected to a negative pressure device, characterized in that: The main body (1) of the hole forming device includes a storage cylinder (11) and an installation cylinder (12). An opening is provided between the opposite ends of the storage cylinder (11) and the installation cylinder (12), and the connection part (2) is used to cooperate at the opening. The storage cylinder (11) has a connector (13) at the top and a filter screen (3) at the opening of the connector (13) inside. The bottom of the mounting cylinder (12) is detachably connected to several perforated tubes (5) via a fastener (4).

2. The agarose gel negative pressure pore forming device according to claim 1, characterized in that: The filter screen (3) has a cylindrical structure. One end of the filter screen (3) has an opening that abuts against the inner side of the mounting cylinder (12), and the other end is provided with a compression spring (14) between it and the storage cylinder (11). The filter screen (3) is used to hold broken gel blocks.

3. The agarose gel negative pressure pore forming device according to claim 1, characterized in that: The fastener (4) includes a connecting inner cylinder (41) and a connecting outer cylinder (42). The connecting inner cylinder (41) is fixedly connected to the bottom of the mounting cylinder (12) and is threadedly connected to the connecting outer cylinder (42) by internal and external threads (43). An elastic clamp (44) is also provided inside the connecting inner cylinder (41).

4. The agarose gel negative pressure pore forming device according to claim 3, characterized in that: The elastic gripper (44) has a cylindrical structure and its top periphery extends outward to form a protrusion (441). The top of the elastic gripper (44) is provided with a number of deformation grooves (442) equidistantly along the axial direction, and the perforated tube (5) is slidably connected inside it.

5. The agarose gel negative pressure pore forming device according to claim 4, characterized in that: The top of the connecting outer cylinder (42) is recessed to form a clearance hole, and the inner wall of the clearance hole extends downward to form an extension (421). The extension (421) corresponds to the protrusion (441), and a guide slope (422) is provided between their opposing ends. The connecting outer cylinder (42) abuts against the protrusion (441) through the extension (421) so that the elastic claw (44) clamps the perforated tube (5).

6. The agarose gel negative pressure pore forming device according to claim 1, characterized in that: A sealing ring is provided between one end of the perforated tube (5) and the mounting cylinder (12).

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