Cell recovery device

By designing a heating copper tube and a homogenizing mechanism, combined with motor drive and fan blade agitation, the problem of uneven temperature in cryopreservation tubes was solved, improving the efficiency and activity consistency of cell revival.

CN223535082UActive Publication Date: 2025-11-11HEFEI TONGMIAN BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202423007838.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-03
Publication Date
2025-11-11
Estimated Expiration
2034-12-03

AI Technical Summary

Technical Problem

During the simultaneous thawing of multiple cryovials, contact between the cryovials leads to uneven temperature distribution, affecting cell thawing efficiency and causing instability in cell viability.

Method used

A cell resuscitation device is used, which utilizes a heating copper tube and a homogenizing mechanism. The water temperature is homogenized by a motor-driven rotating shaft and fan blades, and the vertical reciprocating movement of the cryopreservation tube is achieved through the sliding connection of the placement mechanism, ensuring that the cryopreservation tube is in full contact with the water.

Benefits of technology

This achieved temperature stability and consistency during the cryopreservation process, improving the efficiency and activity consistency of cell thawing.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of cell resuscitation, and discloses a cell resuscitation device which comprises a shell, a plurality of groups of bases are uniformly and fixedly connected to the bottom end of the shell, a heating copper pipe is fixedly connected in the shell, and a sealing cover is connected to the top end of the shell in an inserted manner; the device further comprises a placing mechanism, the placing mechanism vertically slides on the top of the inner wall of the shell and comprises a vertical strip, the vertical strip is vertically and slidably connected with a sleeve block, and the outer wall of the sleeve block is provided with a guide notch which fluctuates annularly. According to the device, the temperature of the internal water body can be relatively stable after the water body is in contact with the cryopreservation tube by utilizing the homogenizing mechanism, so that the cryopreservation tube can be recovered orderly, and the water body can be uniformly stirred when the fan blades stir the water body under the sliding connection of the homogenizing mechanism and the placing mechanism. And the sleeve block can be driven to perform vertical reciprocating displacement under the sliding connection of the guide notch and the sliding block, so that the cryopreservation tube can be in full contact with the water body, and the cell recovery efficiency is improved.
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Description

Technical Field

[0001] This utility model relates to the field of cell resuscitation technology, and in particular to a cell resuscitation device. Background Technology

[0002] Cell cryopreservation and thawing are common techniques in cell research, and cell thawing is a crucial step affecting cell viability. Currently, the most common thawing method is water bath heating, which allows the cryopreserved cell suspension to thaw rapidly, enabling subsequent experiments.

[0003] However, during the thawing process, multiple cryovials need to be thawed simultaneously. Since the cryovials come into contact with each other, the water temperature in the cryovial area will drop, and the water body is relatively fixed, which makes the cryovials prone to uneven heating, resulting in different cell thawing efficiencies. This leads to unstable cell activity in the same water bath and affects the overall cell thawing efficiency.

[0004] To this end, we propose a cell resuscitation device. Utility Model Content

[0005] To address the technical problem that when multiple cryovials are simultaneously thawed, the interaction between the cryovials causes a drop in temperature within the affected area, thereby reducing the overall cell thaw efficiency, this invention provides a cell thaw device.

[0006] This utility model is achieved using the following technical solution: a cell resuscitation device, comprising:

[0007] The shell has multiple sets of bases evenly and fixedly connected to its bottom end, a heating copper tube is fixedly connected inside the shell, and a cap is inserted and connected to its top end.

[0008] Also includes:

[0009] The placement mechanism slides vertically on the top of the inner wall of the housing, and includes a vertical bar. The vertical bar is vertically slidably connected to a sleeve block, and the outer wall of the sleeve block is provided with an annular undulating guide groove.

[0010] A uniformizing mechanism is slidably connected to the guide slot and is located at the bottom of the housing.

[0011] Through the above technical solution, the temperature of the water inside the shell can be homogenized by using a homogenizing mechanism in conjunction with a heating copper tube, so as to ensure that the resuscitation efficiency is consistent during the resuscitation operation, thereby achieving the consistency of cell activity and improving the overall resuscitation efficiency. In order to further improve the resuscitation efficiency of the equipment, a placement mechanism that is slidably connected to the homogenizing mechanism can be used to move vertically and accelerate the resuscitation of internal cells.

[0012] As a further improvement to the above solution, the uniform mechanism includes a motor chamber, which is fixed to the bottom of the housing. A motor is fixedly connected inside the motor chamber, and a rotating shaft is fixedly connected to the top of the motor. A sealing rubber ring is provided at the connection between the motor and the motor chamber.

[0013] The above technical solution utilizes a sealing rubber ring to prevent internal water leakage and protect the normal operation of the motor.

[0014] As a further improvement to the above solution, a rotating block is fixedly connected to the outer wall of the rotating shaft, fan blades are uniformly fixedly connected to the outer wall of the rotating block, an L-plate is fixedly connected to the top of the rotating shaft, and a slider is fixedly connected to the inner side of the top of the L-plate, the slider sliding in the guide groove.

[0015] Through the above technical solution, by utilizing the sliding of the slider and the guide slot, the entire sleeve can be pulled to rotate with the L-plate, allowing for adaptive vertical reciprocating undulating displacement, thereby further improving cell resuscitation efficiency.

[0016] As a further improvement to the above solution, the placement mechanism also includes multiple sets of placement slots uniformly fixedly connected inside the sleeve block, and the outer walls of the multiple sets of placement slots are provided with communicating slots, and cryopreservation tubes are placed inside the placement slots.

[0017] As a further improvement to the above solution, a limiting block is threadedly connected to the middle of the sleeve block, the top of the limiting block is provided with a groove, and the bottom of the limiting block covers multiple sets of placement slots.

[0018] Through the above technical solution, the bottom of the limiting block can completely cover the top of all the placement slots, achieving complete limiting of the placement slots and preventing them from falling off during the recovery operation.

[0019] As a further improvement to the above solution, water is placed inside the shell, and the height of the water is lower than the height of the lowest point of the vertical sliding groove.

[0020] The above technical solution ensures that the sleeve can be fully immersed in water when it is moved vertically back and forth.

[0021] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0022] This invention utilizes a uniform mechanism to ensure that the temperature of the water inside the cryopreservation tube remains relatively stable after the water comes into contact with it, thereby ensuring the orderly thawing of the cryopreservation tube. Furthermore, with the sliding connection between the uniform mechanism and the placement mechanism, when the fan blades agitate the water, the guide slot and the slider can also drive the sleeve block to move vertically back and forth, enabling the cryopreservation tube to fully contact the water and improving the efficiency of cell thawing. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of a cell resuscitation device provided in Embodiment 1 of this utility model;

[0024] Figure 2 This is a front cross-sectional view of the present invention.

[0025] Figure 3 This is a schematic diagram of the internal structural connection state of this utility model.

[0026] Explanation of key symbols:

[0027] 1. Housing; 2. Base; 3. Heating copper tube; 4. Motor chamber; 5. Motor; 6. Shaft; 7. Rotating block; 8. Fan blade; 9. Sleeve block; 10. Placement slot; 11. Limiting block; 12. Guide slot; 13. Cover; 14. Sliding block; 15. L-plate. Detailed Implementation

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0029] Example 1:

[0030] Please combine Figures 1-3 A cell resuscitation device according to this embodiment includes:

[0031] The shell 1 has multiple sets of bases 2 evenly fixedly connected to the bottom end of the shell 1, a heating copper tube 3 is fixedly connected inside the shell 1, and a cover 13 is inserted and connected to the top end of the shell 1.

[0032] Also includes:

[0033] The placement mechanism slides vertically on the top of the inner wall of the housing 1, and includes a vertical bar. The vertical bar is vertically slidably connected to a sleeve block 9. The outer wall of the sleeve block 9 is provided with an annular undulating guide groove 12.

[0034] The uniformizing mechanism is slidably connected to the guide slot 12 and is located at the bottom of the housing 1.

[0035] The uniform mechanism includes a motor chamber 4, which is fixed to the bottom of the housing 1. A motor 5 is fixedly connected inside the motor chamber 4, and a rotating shaft 6 is fixedly connected to the top of the motor 5. A sealing rubber ring is provided at the connection between the motor 5 and the motor chamber 4.

[0036] A rotating block 7 is fixedly connected to the outer wall of the rotating shaft 6. Fan blades 8 are evenly fixedly connected to the outer wall of the rotating block 7. An L-plate 15 is fixedly connected to the top of the rotating shaft 6. A slider 14 is fixedly connected to the inner side of the top of the L-plate 15. The slider 14 slides in the guide slot 12.

[0037] The implementation principle of a cell resuscitation device in this application embodiment is as follows:

[0038] The motor 5 drives the shaft 6 to rotate, which in turn drives the rotating block 7 and the fan blade 8 to rotate. This causes the water poured into the tube to heat up under the heating effect of the heating copper tube 3. The fan blade 8 interacts with the heated water and the cryopreservation tube, allowing the water to rotate and the overall water temperature to be uniform, thus ensuring the cryopreservation tube's recovery efficiency.

[0039] L-plate 15 rotates with the rotation of shaft 6, and L-plate 15 is slidably connected to guide slot 12 by slider 14, so that sleeve 9 can slide vertically on inner wall of housing 1, and drive multiple sets of cryopreservation tubes to move, so as to improve their recovery efficiency.

[0040] Example 2:

[0041] Combination Figures 1-3 This embodiment is an improvement on embodiment 1, further described in the following aspects:

[0042] The placement mechanism also includes multiple sets of placement slots 10 that are uniformly fixedly connected inside the sleeve block 9. The outer walls of the multiple sets of placement slots 10 are provided with communicating slots, and cryopreservation tubes are placed inside the placement slots 10.

[0043] The middle of the sleeve 9 is threadedly connected to a limiting block 11. The top of the limiting block 11 has a groove, and the bottom of the limiting block 11 covers multiple sets of placement slots 10.

[0044] Water is placed inside the shell 1, and the water level is lower than the lowest point of the vertical sliding groove 10.

[0045] The implementation principle of this embodiment is as follows:

[0046] Open the housing 1 by holding the cap 13, disconnect the cap 13 from the housing 1, rotate the limiting block 11 in the opposite direction to disconnect the threaded connection with the sleeve block 9, then pour an appropriate amount of clean water into the housing 1, place the cryopreservation tube to be reviewed in the placement slot 10, and rotate the limiting block 11 again to fix and limit the cryopreservation tube placed in the placement slot 10. Then insert the cap 13 into the inner wall of the housing 1 to complete the sealing state and prevent the temperature from losing too quickly.

[0047] Once the revival process is complete, simply reverse the steps described above to retrieve the revival cryopreserved tube.

[0048] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A cell resuscitation device, comprising: The shell has multiple sets of bases evenly and fixedly connected to its bottom end, a heating copper tube is fixedly connected inside the shell, and a cap is inserted and connected to its top end. Its characteristic is that it further includes: The placement mechanism slides vertically on the top of the inner wall of the housing, and includes a vertical bar. The vertical bar is vertically slidably connected to a sleeve block, and the outer wall of the sleeve block is provided with an annular undulating guide groove. A uniformizing mechanism is slidably connected to the guide slot and is located at the bottom of the housing.

2. The cell resuscitation device as described in claim 1, characterized in that, The uniformizing mechanism includes a motor chamber, which is fixed to the bottom of the housing. A motor is fixedly connected inside the motor chamber, and a rotating shaft is fixedly connected to the top of the motor. A sealing rubber ring is provided at the connection between the motor and the motor chamber.

3. The cell resuscitation device as described in claim 2, characterized in that, A rotating block is fixedly connected to the outer wall of the rotating shaft, and fan blades are evenly fixedly connected to the outer wall of the rotating block. An L-plate is fixedly connected to the top of the rotating shaft, and a slider is fixedly connected to the inner side of the top of the L-plate. The slider slides in the guide groove.

4. The cell resuscitation device as described in claim 1, characterized in that, The placement mechanism also includes multiple sets of placement slots that are uniformly fixedly connected inside the sleeve block. The outer walls of the multiple sets of placement slots are provided with communicating slots, and cryopreservation tubes are placed inside the placement slots.

5. The cell resuscitation device as described in claim 4, characterized in that, The sleeve block is threadedly connected to a limiting block in the middle. The limiting block has a groove at its top and multiple placement slots at its bottom.

6. The cell resuscitation device as described in claim 2, characterized in that, The shell contains water, and the water level is lower than the lowest point of the vertical sliding groove.