Full-automatic sewage filtration nucleic acid extraction instrument

Abstract

The embodiment of the utility model provides a full-automatic sewage filtration nucleic acid extraction instrument. Comprising a base internally provided with a push-pull groove; the container assembly is arranged in the push-pull groove; the reagent strip assembly is connected with the base, and the reagent strip assembly can move relative to the base in the first direction; the filtering assembly and the nucleic acid extraction assembly are both connected with the base, the filtering assembly and the nucleic acid extraction assembly are located on the two opposite sides of the base in the first direction, and the filtering assembly can move in the second direction relative to the base; wherein the filter assembly is configured to extend into the container assembly or be separated from the container assembly in a third direction, the filter assembly is further configured to extend into the reagent strip assembly or be separated from the reagent strip assembly in the third direction, and the nucleic acid extraction assembly is configured to extend into the reagent strip assembly or be separated from the reagent strip assembly in the third direction.

Description

Technical Field

[0001] This application relates to the field of biological detection technology, and in particular to a fully automated wastewater filtration nucleic acid extractor. Background Technology

[0002] In recent years, with the rapid development of molecular biology, nucleic acid-based molecular diagnostics and detection technologies have played an increasingly important role in many fields. Currently, magnetic bead extraction is commonly used for nucleic acid extraction.

[0003] The enrichment and concentration of viruses in wastewater and the detection of nucleic acids must be carried out in accordance with relevant industry standards, which mainly include wastewater sampling, virus inactivation, virus enrichment and concentration, and nucleic acid extraction and detection.

[0004] In related technologies, after water sample collection and enrichment / concentration, the water samples need to be manually transported to a specialized laboratory for subsequent nucleic acid extraction and testing to obtain information about the viruses contained in the wastewater samples. This makes the entire process cumbersome and requires manual intervention, thus hindering work efficiency. Utility Model Content

[0005] This application provides a fully automated wastewater filtration nucleic acid extractor, which integrates enrichment and concentration functions and nucleic acid extraction functions. It can automatically obtain virus information from wastewater samples, thereby improving work efficiency.

[0006] The fully automatic wastewater filtration nucleic acid extractor includes: a base with a push-pull groove inside; a container assembly disposed within the push-pull groove; a reagent strip assembly connected to the base, the reagent strip assembly being movable relative to the base along a first direction; a filter assembly and a nucleic acid extraction assembly, both connected to the base, the filter assembly and the nucleic acid extraction assembly being located on opposite sides of the base in the first direction, the filter assembly being movable relative to the base along a second direction; wherein, the filter assembly is configured to extend into or detach from the container assembly in a third direction, the filter assembly is further configured to extend into or detach from the reagent strip assembly in a third direction, and the nucleic acid extraction assembly is configured to extend into or detach from the reagent strip assembly in a third direction, the first direction, the second direction, and the third direction being perpendicular to each other.

[0007] The fully automated wastewater filtration nucleic acid extractor of this application integrates sample enrichment and nucleic acid detection functions after sample enrichment. The sample enrichment, transfer, and subsequent nucleic acid extraction processes can be largely automated, thereby shortening the detection cycle, simplifying steps, reducing manual intervention, and thus improving work efficiency. Attached Figure Description

[0008] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0009] Figure 1 This is a schematic diagram of the structure of a fully automated wastewater filtration nucleic acid extractor according to an embodiment of this application;

[0010] Figure 2 This is a schematic diagram of the structure of a base according to an embodiment of this application;

[0011] Figure 3 This is a schematic diagram of the structure of a filter assembly and a first side plate according to an embodiment of this application;

[0012] Figure 4 This is a partial structural schematic diagram of a filtering component according to an embodiment of this application;

[0013] Figure 5 This is a schematic diagram of the structure of a container component according to an embodiment of this application;

[0014] Figure 6 This is a schematic diagram of the structure of a nucleic acid extraction component according to an embodiment of this application;

[0015] Figure 7 This is a schematic diagram of the structure of the first scaffold and the second scaffold of a nucleic acid extraction component according to an embodiment of this application;

[0016] Figure 8 This is a schematic diagram of the structure of the third support, magnetic sleeve, magnetic rod, and other components of a nucleic acid extraction assembly according to an embodiment of this application.

[0017] Figure 9 for Figure 8 The diagram shows a structural schematic from another perspective;

[0018] Figure 10 This is a schematic diagram of the structure of a reagent strip assembly according to an embodiment of this application. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0020] In the description of this application, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the accompanying drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0021] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as implying or suggesting relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship.

[0022] In the description of this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0023] like Figure 1As shown, the fully automatic wastewater filtration nucleic acid extractor 10 includes a base 100, a container assembly 200, a reagent strip assembly 300, a filter assembly 400, and a nucleic acid extraction assembly 500. The base 100 has a push-pull groove 101 inside, and the container assembly 200 is disposed within the push-pull groove 101. The reagent strip assembly 300 is connected to the base 100 and can move relative to the base 100 along a first direction X. The filter assembly 400 and the nucleic acid extraction assembly 500 are both connected to the base 100 and are located on opposite sides of the base 100 in the first direction X. The filter assembly 400 can move relative to the base 100 along a second direction Y. The filter assembly 400 is configured to extend into or detach from the container assembly 200 in the third direction Z. The filter assembly 400 is also configured to extend into or detach from the reagent strip assembly 300 in the third direction Z. The nucleic acid extraction assembly 500 is configured to extend into or detach from the reagent strip assembly 300 in the third direction Z. The first direction X, the second direction Y, and the third direction Z are perpendicular to each other.

[0024] The base 100 serves as the substrate for the fully automated wastewater filtration nucleic acid extractor 10, and is used to mount other components. The container assembly 200 is used to hold the sample to be enriched, such as domestic wastewater. The reagent strip assembly 300 is used to provide multiple reagent wells with different functions. For example, the reagent strip assembly 300 can have sample reagent wells, magnetic bead reagent wells, cleaning reagent wells, elution reagent wells, and magnetic sleeve reagent wells. The sample reagent wells are used to hold the enriched wastewater sample, the magnetic bead reagent wells are used to place magnetic beads, the magnetic sleeve reagent wells are used to place magnetic sleeves 570, the cleaning reagent wells are used to place cleaning solution, and the elution reagent wells are used to place elution solution. The number of wells and reagent types on the reagent strip assembly 300 can be flexibly designed according to the actual detection process, and this application does not impose any restrictions on this.

[0025] The filter assembly 400 enables sample enrichment and transfer. Firstly, the filter assembly 400 can move relative to the base 100 along a second direction Y, allowing it to align with the container assembly 200 in a third direction Z. The filter assembly 400 extends into the container assembly 200 in the third direction Z to enrich the sample within. After enrichment, the filter assembly 400 detaches from the container assembly 200 along the third direction Z. At this point, the enriched target component adheres to the filter assembly 400. Secondly, the reagent strip assembly 300 can move relative to the base 100 along a first direction X, with the filter assembly 400 located on one side of the base 100 in the first direction X, allowing the filter assembly 400 and the reagent strip assembly 300 to align in the third direction Z. After enrichment, the filter assembly 400 and reagent strip assembly 300 are first aligned in the third direction (Z). Then, the filter assembly 400 extends into the reagent strip assembly 300 along the third direction (Z), transferring the target component into the reagent strip assembly 300. After the transfer is complete, the filter assembly 400 detaches from the reagent strip assembly 300 along the third direction (Z). At this point, the enriched target component is transferred to the corresponding sample reagent well on the reagent strip assembly 300.

[0026] The nucleic acid extraction component 500 is used to extract nucleic acids from the sample reagent wells on the reagent strip assembly 300. Since the reagent strip assembly 300 is movable relative to the base 100 along the first direction X, and the nucleic acid extraction component 500 is located on the other side of the base 100 in the first direction X, the nucleic acid extraction component 500 and the reagent strip assembly 300 can be aligned in the third direction Z. Thus, the nucleic acid extraction component 500 can extend into the sample reagent wells of the reagent strip assembly 300 along the third direction Z to extract nucleic acids from the sample.

[0027] The fully automated wastewater filtration nucleic acid extractor 10 of this application integrates sample enrichment and nucleic acid detection functions after sample enrichment. The sample enrichment, transfer after enrichment, and subsequent nucleic acid extraction can be basically automated, thereby shortening the detection cycle, simplifying the steps, reducing manual intervention, and thus improving the efficiency of nucleic acid extraction.

[0028] In some embodiments, the container assembly 200 can also move relative to the base 100 along a second direction Y, so that the container assembly 200 extends into or detaches from the push-pull groove 101. For example, the container assembly 200 can be movably connected to the base 100 via components such as a slide rail or a drive structure. In this embodiment, the container assembly 200 is configured to be movably connected to the base 100. When it is necessary to store a sample, the container assembly 200 can be pulled out from the push-pull groove 101 to facilitate sample placement. When sample enrichment is performed, the container assembly 200 can be pushed into the push-pull groove 101 to ensure convenient alignment between the filter assembly 400 and the container assembly 200. In this way, on the one hand, the convenience of the container assembly 200 for storing samples can be improved, and on the other hand, it is beneficial to further improve work efficiency.

[0029] In some embodiments, such as Figure 1 As shown, the nucleic acid extraction component 500 can also move relative to the base 100 along the first direction X, so that the nucleic acid extraction component 500 moves closer to or further away from the filter component 400. Since the nucleic acid extraction component 500 is located on one side of the base 100 in the first direction X, and the nucleic acid extraction component 500 needs to be sequentially aligned with the different functional wells of the reagent strip assembly 300 in the third direction Z, the nucleic acid extraction component 500 can compensate for the insufficient travel distance of the reagent strip assembly 300 in the first direction X, thereby improving the reliability and convenience of their alignment.

[0030] Optionally, such as Figure 1 As shown, the fully automated wastewater filtration nucleic acid extractor 10 includes a first drive structure (not shown) and a second drive structure 600. The container assembly 200 is connected to the base 100 via the first drive structure, thereby enabling the container assembly 200 to move relative to the base 100 along the second direction Y. The nucleic acid extraction assembly 500 is connected to the base 100 via the second drive structure 600, thereby enabling the nucleic acid extraction assembly 500 to move relative to the base 100 along the first direction X. This improves the automation and efficiency of nucleic acid extraction.

[0031] Optionally, the first drive structure and the second drive structure 600 can be one of the following: synchronous belt drive, ball screw drive, cylinder drive, hydraulic cylinder drive, etc., and this application does not limit this.

[0032] In some embodiments, such as Figures 1 to 4As shown, the fully automated wastewater filtration nucleic acid extractor 10 also includes a third drive structure 700 and a fourth drive structure 800. The reagent strip assembly 300 is connected to the base 100 via the third drive structure 700, and the filter assembly 400 is connected to the base 100 via the fourth drive structure 800. This allows for movement of the reagent strip assembly 300 relative to the base along the first direction X and movement of the filter assembly 400 relative to the base along the second direction Y, thereby improving the automation and efficiency of nucleic acid extraction.

[0033] In some embodiments, such as Figures 1 to 3 As shown, the base 100 includes a base plate 110, a first side plate 120, a second side plate 130, and a top plate 140. The first side plate 120 and the second side plate 130 are both connected to the base plate 110 and located on opposite sides of the base plate 110 in the first direction X. The top plate 140 is disposed opposite to the base plate 110 and connected to the first side plate 120 and the second side plate 130. The top plate 140, the base plate 110, the first side plate 120, and the second side plate 130 together form a push-pull groove 101. The reagent strip assembly 300 is movably connected to the top plate 140 and is located on the side of the top plate 140 away from the push-pull groove 101.

[0034] This embodiment proposes a specific structure for the base 100. By forming a push-pull groove 101, the space of the base 100 can be rationally utilized, reducing its volume and achieving miniaturization. Furthermore, the reagent strip assembly 300 is movably disposed on the upper surface of the top plate 140, allowing both the reagent strip assembly 300 and the container assembly 200 to rationally utilize the space of the base 100. The two are designed in a layered manner, which improves the convenience of arranging the reagent strip assembly 300 and the container assembly 200. In addition, because the reagent strip assembly 300 and the container assembly 200 are designed in a layered manner, they will not interfere with each other, thus facilitating the movable connection between the reagent strip assembly 300, the container assembly 200, and the base 100. This further improves the convenience of arranging components such as the drive structure and guide rails.

[0035] In some embodiments, such as Figure 1 and Figure 2 As shown, the second side plate 130 is located between the bottom plate 110 and the top plate 140. The first side plate 120 includes a first part 121 and a second part 122. The first part 121 is located between the bottom plate 110 and the top plate 140. The second part 122 protrudes from the top plate 140 and extends in the third direction Z. The filter assembly 400 is movably connected to the second part 122, and the nucleic acid extraction assembly 500 is movably connected to the top plate 140.

[0036] This embodiment proposes a connection method between the filter assembly 400 and the nucleic acid extraction assembly 500 and the base 100. Specifically, the height of the first side plate 120 is higher than the height of the top plate 140 and the height of the second side plate 130. This allows the filter assembly 400 to be movably connected to the base 100 via the second part 122 of the first side plate 120. It also allows for alignment of the filter assembly 400 with the reagent strip assembly 300 and the container assembly 200 in the third direction Z, thereby improving the convenience of arranging the filter assembly 400. Furthermore, the nucleic acid extraction assembly 500 is movably connected to the top plate 140, allowing the nucleic acid extraction assembly 500 to move along the first direction X on the top plate 140, which also improves the convenience of arranging the nucleic acid extraction assembly 500.

[0037] In one specific embodiment, such as Figure 1 and Figure 2 As shown, the nucleic acid extraction component 500 is connected to the top plate 140 via a second drive structure 600. The second drive structure 600 includes a first motor 610 connected to the top plate 140, a synchronous belt 620 connected to the output end of the first motor 610, a first slider 630 disposed on the synchronous belt 620, and a first guide rail 640 disposed on the upper surface of the top plate 140. The first slider 630 is slidably disposed on the first guide rail 640, and the nucleic acid extraction component 500 is mounted on the first slider 630. Thus, linear motion of the nucleic acid extraction component 500 relative to the base 100 in the first direction X is realized.

[0038] In one specific embodiment, such as Figure 1 and Figure 2 As shown, the reagent strip assembly 300 is connected to the top plate 140 via a third drive structure 700. The third drive structure 700 includes a second motor 710 connected to the second side plate 130, a first lead screw 720 connected to the output end of the second motor 710, and a second slider 730 sleeved on the first lead screw 720. The reagent strip assembly 300 is connected to the second slider 730. Thus, linear motion of the reagent strip assembly 300 relative to the base 100 in the first direction X is achieved.

[0039] Optionally, a second guide rail 740 is also provided on the top plate, and the reagent strip assembly 300 is slidably disposed on the second guide rail 740, which helps to improve the stability of the movement of the reagent strip assembly 300.

[0040] In one specific embodiment, such as Figure 1 , Figure 3 and Figure 4As shown, the filter assembly 400 is connected to the second part 122 of the first side plate 120 via a fourth drive structure 800. The fourth drive structure 800 includes a third motor 810 connected to the second part 122, a gear 820 connected to the output end of the third motor 810, and a rack (not shown) meshing with the gear 820. The filter assembly 400 is connected to the rack. Thus, linear motion of the filter assembly 400 relative to the base 100 in the second direction Y is achieved.

[0041] In some embodiments, such as Figure 3 , Figure 4 As shown, the filter assembly 400 includes a filter element 410, a drive pump 420 communicating with the filter element 410, and a fifth drive structure 430 connected to the filter element 410. The filter element 410 is configured to enrich the sample within the container assembly 200, and the fifth drive structure 430 is configured to drive the filter element 410 to move along the third direction Z. Thus, the filter element 410 can extend into the container assembly 200 under the drive of the fifth drive structure 430, and the drive pump 420 can then drive the filter element 410 to enrich and concentrate the sample within the container assembly 200. The enriched and concentrated target component is then transferred onto the filter element 410. Next, the fifth drive structure 430 drives the filter element 410 to detach from the container assembly 200. Afterward, the filter assembly 400 successfully aligns with the reagent strip assembly 300 in the third direction Z, and the filter element 410 can extend into the reagent strip assembly 300 under the drive of the fifth drive structure 430, transferring the target component on the filter element 410 into the reagent strip assembly 300. In this embodiment, a filter membrane is used to enrich and concentrate the sample, thereby improving the convenience of enrichment and sample transfer. Optionally, the filter element 410 can be a quick-release filter cartridge, and the drive pump 420 can be a peristaltic pump.

[0042] Optionally, such as Figure 3 , Figure 4 As shown, the filter assembly 400 also includes an enrichment mounting plate 440, which is connected to the output end of the fourth drive structure 800, and the fixed end of the fifth drive structure 430 is connected to the enrichment mounting plate 440. This allows for the installation and fixation of the filter element 410 and the fifth drive structure 430. The fifth drive structure 430 can be a ball screw drive structure.

[0043] In some embodiments, such as Figure 1 , Figure 5 As shown, the container assembly 200 includes a mounting plate 210 and a plurality of containers 220 spaced apart along the second direction Y on the mounting plate 210. The containers 220 are used to place samples. Optionally, the containers 220 can be beakers, measuring cylinders, etc., and this application does not limit them.

[0044] Furthermore, such as Figure 1 , Figure 3 As shown, the number of filter elements 410, drive pump 420, and fifth drive structure 430 is multiple and equal to the number of containers 220. Each fifth drive structure 430 drives a filter element 410 to extend into or detach from a container 220 along a third direction Z. Therefore, sample enrichment within multiple containers 220 can be completed simultaneously, thereby improving enrichment efficiency.

[0045] In some embodiments, such as Figure 1 , Figures 6 to 9 As shown, the nucleic acid extraction component 500 includes a first support 510, a second support 520, a third support 530, a magnetic rod connecting plate 540, at least one magnetic rod 550, a magnetic sleeve connecting plate 560, and at least one magnetic sleeve 570. The first support 510 is connected to the base 100 and extends along a third direction Z. The second support 520 is movably connected to the first support 510 along a second direction Y. The third support 530 is movably connected to the second support 520 along a third direction Z. The magnetic rod connecting plate 540 is movably connected to the third support 530 along a third direction Z. The magnetic rod 550 is disposed on the magnetic rod connecting plate 540. The magnetic sleeve connecting plate 560 is located below the magnetic rod connecting plate 540 and is fixedly connected to the third support 530. The magnetic sleeve 570 is connected to the magnetic sleeve connecting plate 560 and is arranged in a one-to-one correspondence with the magnetic rod 550. The magnetic rod connecting plate 540 can reciprocate relative to the magnetic sleeve connecting plate 560 in the third direction Z, so that the magnetic rod 550 extends into or detaches from the magnetic sleeve 570.

[0046] This application proposes a specific structure for a nucleic acid extraction component 500. Specifically, the first support 510 can connect the nucleic acid extraction component 500 to the base 100. For example, the first support 510 can be fixedly connected to the base 100, or, as mentioned above, the first support 510 can be connected to the base 100 through the second drive structure 600, so that the first support 510 can move relative to the base 100 along the first direction X. In this case, the first support 510 can also adjust the position of the magnetic sleeve 570 and the magnetic rod 550 in the first direction X.

[0047] Furthermore, the second support 520 can move relative to the first support 510 along the second direction Y, thereby driving the magnetic sleeve 570 and the magnetic rod 550 to move along the second direction Y, thus enabling position adjustment of the magnetic sleeve 570 and the magnetic rod 550 in the second direction Y. The second support 520 can be connected to the first support 510 through the sixth drive structure 900, such as... Figure 1 As shown, the sixth drive structure 900 is a synchronous belt drive structure.

[0048] Furthermore, the third support 530 can realize the position adjustment of the magnetic sleeve 570 in the third direction Z and the automatic loading function of the magnetic sleeve 570. As mentioned above, the reagent strip assembly 300 is provided with multiple reagent holes with different functions, and the magnetic sleeve 570 is placed in the magnetic sleeve reagent hole in advance. After the magnetic sleeve connecting plate 560 is aligned with the magnetic sleeve reagent hole of the reagent strip assembly 300, the third support 530 can drive the magnetic sleeve connecting plate 560 to move in the third direction Z, so that the magnetic sleeve connecting plate 560 automatically loads the magnetic sleeve 570 located in the magnetic sleeve reagent hole, thereby realizing the automation of the installation of the magnetic sleeve 570 and the magnetic sleeve connecting plate 560, which is conducive to further improving the extraction efficiency. Optionally, the third support 530 can be connected to the second support 520 through the seventh drive structure 1000, such as Figure 7 As shown, the seventh drive structure 1000 is a ball screw drive structure.

[0049] Furthermore, the magnetic rod connecting plate 540 is movably connected to the third bracket 530 along the third direction Z, and the magnetic rod 550 is disposed on the magnetic rod connecting plate 540. In this way, the magnetic rod 550 can move independently relative to the magnetic sleeve 570 along the third direction Z, thereby allowing the magnetic rod 550 to extend into or detach from the magnetic sleeve 570. When the magnetic rod 550 extends into the magnetic sleeve 570, it can attract magnetic beads to the outer surface of the magnetic sleeve 570. When the magnetic rod 550 detaches from the magnetic sleeve 570, the magnetism disappears, and the magnetic beads on the outer surface of the magnetic sleeve 570 fall off. Optionally, the magnetic rod connecting plate 540 can be connected to the third bracket 530 via the eighth driving structure 1100, such as... Figure 6 and Figure 8 As shown, the eighth drive structure 1100 is a ball screw drive structure.

[0050] In some embodiments, such as Figure 8 As shown, the magnetic sleeve 570 and the magnetic sleeve connecting plate 560 are rotatably connected. Specifically, the nucleic acid extraction assembly 500 also includes a ninth drive structure 1200, which includes a fourth motor 1210 connected to the magnetic sleeve connecting plate 560, a gear transmission assembly 1220 connected to the output end of the fourth motor 1210, and a plurality of rotating shafts 1230 connected to the gear transmission assembly 1220. At least a portion of the gear transmission assembly 1220 is located within the magnetic sleeve connecting plate 560. The rotating shafts 1230 are arranged in a one-to-one correspondence with the magnetic sleeves 570, with each magnetic sleeve 570 connected to a rotating shaft 1230. The rotating shafts 1230 are configured to drive the magnetic sleeves 570 to rotate under the drive of the fourth motor 1210. In this way, the fourth motor 1210 can drive the magnetic sleeves 570 to rotate, stirring the liquid in the well, thereby improving the reliability of nucleic acid extraction.

[0051] It is easy to understand that the gear transmission assembly 1220 includes multiple gears, and the gear transmission assembly 1220 can convert the power of one output shaft of the fourth motor 1210 into the power of multiple rotating shafts 1230.

[0052] In some embodiments, such as Figure 8 , Figure 9 As shown, the nucleic acid extraction assembly 500 also includes a discharge plate 580 and at least one discharge rod 590. The discharge plate 580 is located between the magnetic sleeve 570 and the magnetic sleeve connecting plate 560. One end of the discharge rod 590 is connected to the discharge plate 580, and the other end passes through the magnetic sleeve connecting plate 560 and protrudes from the upper surface of the magnetic sleeve connecting plate 560. When the magnetic rod connecting plate 540 abuts against the end of the discharge rod 590 protruding from the upper surface of the magnetic sleeve connecting plate 560, the discharge rod 590 will be subjected to the squeezing force of the magnetic rod connecting plate 540, thereby driving the discharge plate 580 to move towards the magnetic sleeve 570 until the discharge plate 580 removes the magnetic sleeve 570 from the rotating shaft 1230. This allows for automated disassembly of the magnetic sleeve 570, which helps to further improve the efficiency of nucleic acid extraction and reduce labor costs.

[0053] In some embodiments, such as Figure 10 As shown and referenced Figure 1 The reagent strip assembly 300 includes a mounting base 310 and multiple reagent strips 320. The mounting base 310 has multiple mounting slots 311 spaced apart along the second direction Y, and the number of mounting slots 311 is the same as the number of reagent strips 320. Each reagent strip 320 is mounted on a mounting slot 311, and each reagent strip 320 has multiple functional holes 321 spaced apart along the first direction X. Therefore, the fully automated wastewater filtration nucleic acid extractor 10 of this application can both enrich and concentrate samples and extract nucleic acids after sample enrichment, reducing manual intervention and thus improving work efficiency.

[0054] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A fully automated wastewater filtration nucleic acid extractor, characterized in that, include: The base has an internal sliding groove; A container assembly is disposed within the push-pull groove; A reagent strip assembly is connected to the base, and the reagent strip assembly is movable relative to the base along a first direction; Both the filtering component and the nucleic acid extraction component are connected to the base. The filtering component and the nucleic acid extraction component are located on opposite sides of the base in the first direction. The filtering component is capable of moving relative to the base in the second direction. The filter assembly is configured to extend into or detach from the container assembly from the third direction upwards. The filter assembly is also configured to extend into or detach from the reagent strip assembly from the third direction upwards. The nucleic acid extraction assembly is configured to extend into or detach from the reagent strip assembly from the third direction upwards. The first direction, the second direction, and the third direction are perpendicular to each other.

2. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The container assembly is also capable of moving relative to the base in the second direction to allow the container assembly to extend into or disengage from the push-pull slot. The nucleic acid extraction component can also move relative to the base along the first direction to move the nucleic acid extraction component closer to or further away from the filter component.

3. The fully automated wastewater filtration nucleic acid extractor according to claim 2, characterized in that, The fully automated wastewater filtration nucleic acid extractor includes a first driving structure and a second driving structure. The container assembly is connected to the base through the first driving structure, and the nucleic acid extraction assembly is connected to the base through the second driving structure.

4. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The fully automated wastewater filtration nucleic acid extractor also includes a third driving structure and a fourth driving structure. The reagent strip assembly is connected to the base through the third driving structure, and the filtration assembly is connected to the base through the fourth driving structure.

5. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The filtration assembly includes a filter element, a drive pump communicating with the filter element, and a fifth drive structure connected to the filter element. The filter element is configured to enrich a sample within the container assembly, and the fifth drive structure is configured to drive the filter element to move along the third direction.

6. The fully automated wastewater filtration nucleic acid extractor according to claim 5, characterized in that, The container assembly includes a mounting plate and a plurality of receptacles spaced apart on the mounting plate along the second direction, the receptacles being used to hold samples; The number of the filter element, the drive pump, and the fifth drive structure is multiple and equal to the number of the housing elements. Each of the fifth drive structures drives a filter element to extend into or detach from a housing element along the third direction.

7. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The base includes: Base plate; The first side plate and the second side plate are both connected to the bottom plate and located on opposite sides of the bottom plate in the first direction; A top plate is disposed opposite to the bottom plate and connected to the first side plate and the second side plate. The top plate, the bottom plate, the first side plate and the second side plate together form the push-pull groove. The reagent strip assembly is movably connected to the top plate and is located on the side of the top plate away from the push-pull groove.

8. The fully automated wastewater filtration nucleic acid extractor according to claim 7, characterized in that, The second side plate is located between the bottom plate and the top plate; The first side plate includes a first part and a second part. The first part is located between the bottom plate and the top plate. The second part protrudes from the top plate and extends in the third direction. The filter assembly is movably connected to the second part, and the nucleic acid extraction assembly is movably connected to the top plate.

9. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The nucleic acid extraction component includes: A first bracket is connected to the base and extends in a third direction; The second bracket is movably connected to the first bracket along the second direction; The third support is movably connected to the second support along the third direction; A magnetic rod connecting plate and at least one magnetic rod, wherein the magnetic rod connecting plate is movably connected to the third bracket along the third direction, and the magnetic rod is disposed on the magnetic rod connecting plate; A magnetic sleeve connecting plate and at least one magnetic sleeve, wherein the magnetic sleeve connecting plate is located below the magnetic rod connecting plate and is fixedly connected to the third bracket, and the magnetic sleeve is connected to the magnetic sleeve connecting plate and is arranged in a one-to-one correspondence with the magnetic rod.

10. The fully automated wastewater filtration nucleic acid extractor according to claim 1, characterized in that, The reagent strip assembly includes a mounting base and a plurality of reagent strips. The mounting base is provided with a plurality of mounting slots spaced apart along the second direction. The number of mounting slots is the same as the number of reagent strips. Each reagent strip is mounted on one of the mounting slots. Each reagent strip is provided with a plurality of functional holes spaced apart along the first direction.

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