Methylated DNA fluorescent quantitative detection device

Abstract

The utility model discloses a methylated DNA fluorescent quantitative detection device, which belongs to the technical field of DNA methylation detection and comprises a fluorescent quantitative detector, a motor is fixedly mounted at the rear end of the right side of the fluorescent quantitative detector, the output end of the motor is fixedly connected with a threaded rod, and the surface of the threaded rod is in threaded connection with a threaded sleeve. The motor is started, electric energy is converted into mechanical energy through the motor, the threaded rod is driven to rotate, the threaded sleeve is driven to move longitudinally through the threaded rod, the bearing plate is driven to move through the threaded sleeve under the cooperation of the connecting column, and the sealing plate is driven to move through the bearing plate. When the bearing plate is located outside, a plurality of containers containing samples can be sequentially placed at the top of the rectangular limiting groove, the purpose of detecting the samples at the same time is achieved, and the detection efficiency is greatly improved.

Description

Technical Field

[0001] This invention belongs to the field of DNA methylation detection technology, specifically relating to a quantitative fluorescence detection device for methylated DNA. Background Technology

[0002] DNA methylation is a form of DNA chemical modification that can alter genetic expression without changing the DNA sequence. DNA methylation refers to the covalent bonding of a methyl group to the 5th carbon position of the cytosine of a CpG dinucleotide in the genome under the action of DNA methyltransferases. Numerous studies have shown that DNA methylation can cause changes in chromatin structure, DNA conformation, DNA stability, and the way DNA interacts with proteins, thereby controlling gene expression. Quantitative fluorescence detection devices are required for the detection of DNA methylation.

[0003] Existing quantitative PCR devices release a fluorescent signal when a single probe hybridizes with DNA. The signal intensity is proportional to the amount of PCR product, which allows calculation of the methylation level of the sample. However, since only one sample can be placed at a time, the number of samples that can be processed at one time is limited. This may not meet the needs of high-throughput experiments or large-scale sample processing. Due to the limited number of samples, operators need to perform multiple tests to process all samples, which increases the overall experimental time, especially when processing a large number of samples. Limiting the number of samples may lead to a decrease in experimental efficiency. Therefore, we provide a quantitative PCR device for methylated DNA. Utility Model Content

[0004] The purpose of this invention is to provide a quantitative fluorescence detection device for methylated DNA to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a quantitative fluorescence detection device for methylated DNA, comprising a quantitative fluorescence detector, a motor fixedly installed at the rear end of the right side of the quantitative fluorescence detector, a threaded rod fixedly connected to the output end of the motor, a threaded sleeve threadedly connected to the surface of the threaded rod, a support plate fixedly connected to the top of the threaded sleeve via a connecting post, rectangular limiting grooves provided on both sides of the top of the support plate, a sealing plate provided on the right side of the rectangular limiting groove and outside the quantitative fluorescence detector, an electric telescopic rod fixedly connected to the top of the quantitative fluorescence detector, a connecting plate fixedly connected to the bottom of the electric telescopic rod, and a set of probes provided on both sides of the bottom of the connecting plate.

[0006] Using the above scheme, the motor is started, converting electrical energy into mechanical energy and driving the threaded rod to rotate. The threaded rod drives the threaded sleeve to move longitudinally, and the threaded sleeve, in cooperation with the connecting column, drives the carrier plate to move. The carrier plate drives the sealing plate to move, thereby allowing the carrier plate to extend and retract inside the fluorescence quantitative detector. When the carrier plate is on the outside, multiple containers containing samples can be placed sequentially on top of the rectangular limiting groove, achieving the purpose of simultaneous detection of multiple samples and greatly improving detection efficiency.

[0007] In a preferred embodiment of a quantitative fluorescence detection device for methylated DNA, the front and rear ends of the quantitative fluorescence detector are provided with heat dissipation vents, and the inner cavity of the heat dissipation vents is provided with a dustproof mesh.

[0008] By adopting the above solution and setting up heat dissipation vents, the heat generated during the operation of the quantitative fluorescence detector can be quickly dissipated, achieving efficient heat dissipation and preventing the quantitative fluorescence detector from overheating and causing it to malfunction.

[0009] In a preferred embodiment of a quantitative fluorescence detection device for methylated DNA, a sealing ring is bonded around the perimeter of the sealing plate, and the sealing ring is made of rubber.

[0010] By adopting the above solution, the connection between the quantitative fluorescence detector and the sealing plate is sealed by setting a sealing ring, which prevents external dust from entering the interior of the quantitative fluorescence detector through the gap between the sealing plate and the quantitative fluorescence detector during the detection process, thereby reducing the accuracy of the detection data.

[0011] In a preferred embodiment of a quantitative fluorescence detection device for methylated DNA, a power supply box is fixedly connected to the rear end of the right side of the quantitative fluorescence detector and above the motor, and the inner cavity of the power supply box is equipped with a storage battery.

[0012] By adopting the above solution, the battery is used as a backup power source, so that the device can still maintain normal operation even if the external power supply equipment stops supplying power.

[0013] In a preferred embodiment of a quantitative fluorescence detection device for methylated DNA, one end of the threaded rod is movably connected to a bearing, and one side of the bearing is fixedly connected to one side of the inner cavity of the quantitative fluorescence detector.

[0014] By adopting the above solution, the bearing is used to limit one end of the threaded rod, preventing one end of the threaded rod from being suspended in the air and thus causing vibration.

[0015] In a preferred embodiment of a quantitative fluorescence detection device for methylated DNA, a groove is provided at the bottom of the inner cavity of the quantitative fluorescence detector, and a slider is fixedly connected to the bottom of the threaded sleeve, with the bottom of the slider slidably connected to the inner wall of the groove.

[0016] By adopting the above scheme, the slider is limited by the setting of the slide groove, which assists the threaded sleeve in moving and improves the smoothness of the threaded sleeve movement.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] 1. This utility model starts a motor, which converts electrical energy into mechanical energy and drives a threaded rod to rotate. The threaded rod drives a threaded sleeve to move longitudinally. The threaded sleeve, in cooperation with the connecting column, drives a support plate to move. The support plate drives a sealing plate to move, thereby allowing the support plate to extend and retract inside the fluorescence quantitative detector. When the support plate is on the outside, multiple containers containing samples can be placed sequentially on top of the rectangular limiting groove, achieving the purpose of simultaneous detection of multiple samples and greatly improving detection efficiency.

[0019] 2. This utility model uses an electric telescopic rod to move the connecting plate downwards, which in turn moves the probe downwards until it penetrates into the container. The probe then releases a fluorescent signal, the intensity of which is proportional to the amount of PCR product. Based on this, the methylation degree of the sample can be calculated and displayed on a screen for easy viewing and recording by the user. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 This is a partial schematic diagram from a second perspective of the present invention;

[0022] Figure 3 This is a cross-sectional view of the fluorescence quantitative detector of this utility model.

[0023] In the diagram: 1. Fluorescence quantitative detector; 2. Motor; 3. Threaded rod; 4. Threaded sleeve; 5. Bearing plate; 6. Rectangular limiting groove; 7. Sealing plate; 8. Sealing ring; 9. Electric telescopic rod; 10. Connecting plate; 11. Probe; 12. Power supply box; 13. Battery; 14. Display screen. Detailed Implementation

[0024] Please see Figure 1-3A quantitative fluorescence detection device for methylated DNA includes a quantitative fluorescence detector 1. A motor 2 is fixedly installed at the rear end of the right side of the quantitative fluorescence detector 1. A threaded rod 3 is fixedly connected to the output end of the motor 2. A bearing is movably connected to one end of the threaded rod 3, and one side of the bearing is fixedly connected to one side of the inner cavity of the quantitative fluorescence detector 1. The bearing limits one end of the threaded rod 3 to prevent it from being suspended and vibrating. A threaded sleeve 4 is threadedly connected to the surface of the threaded rod 3. A support plate 5 is fixedly connected to the top of the threaded sleeve 4 through a connecting post. Rectangular limiting grooves 6 are opened on both sides of the top of the support plate 5. A sealing plate 7 is provided on the right side of the rectangular limiting groove 6 and outside the quantitative fluorescence detector 1. Figure 1 As shown, sealing rings 8 are bonded around the edges of the sealing plate 7. The sealing rings 8 are made of rubber. By setting the sealing rings 8, the connection between the fluorescence quantitative detector 1 and the sealing plate 7 is sealed, preventing external dust from entering the interior of the fluorescence quantitative detector 1 through the gap between the sealing plate 7 and the fluorescence quantitative detector 1 during the detection process, thereby reducing the accuracy of the detection data. An electric telescopic rod 9 is fixedly connected to the top of the fluorescence quantitative detector 1, and a connecting plate 10 is fixedly connected to the bottom of the electric telescopic rod 9. A set of probes 11 is provided on both sides of the bottom of the connecting plate 10. When the motor 2 is started, the motor 2 converts electrical energy into mechanical energy and drives the threaded rod 3 to rotate. The threaded rod 3 drives the threaded sleeve 4 to move longitudinally. The threaded sleeve 4 drives the support plate 5 to move with the cooperation of the connecting column. The support plate 5 drives the sealing plate 7 to move, thereby allowing the support plate 5 to extend and retract inside the fluorescence quantitative detector 1. When the support plate 5 is on the outside, multiple containers containing samples can be placed sequentially on the top of the rectangular limiting groove 6 to achieve the purpose of simultaneous detection of multiple samples, greatly improving the detection efficiency.

[0025] See Figure 1 As shown, the front and rear ends of the fluorescence quantitative detector 1 are equipped with heat dissipation vents, and the inner cavity of the heat dissipation vents is equipped with dustproof mesh. Through the design of the heat dissipation vents, the heat generated during operation of the fluorescence quantitative detector 1 can be quickly dissipated, achieving efficient heat dissipation and preventing excessive internal heat from causing the fluorescence quantitative detector 1 to malfunction or freeze. Figure 2 As shown, a power supply box 12 is fixedly connected to the rear right side of the fluorescence quantitative detector 1, above the motor 2. The power supply box 12 contains a battery 13, which serves as a backup power source. Even if the external power supply fails, the device can still operate normally. Figure 3As shown, a sliding groove is provided at the bottom of the inner cavity of the fluorescence quantitative detector 1. A slider is fixedly connected to the bottom of the threaded sleeve 4, and the bottom of the slider is slidably connected to the inner wall of the sliding groove. The sliding groove limits the slider and assists the threaded sleeve 4 in moving, thus improving the smoothness of the movement of the threaded sleeve 4.

[0026] In use, motor 2 is started, converting electrical energy into mechanical energy and driving the threaded rod 3 to rotate. Since the external thread on the surface of the threaded rod 3 matches the internal thread on the inner wall of the threaded sleeve 4, when the threaded rod 3 is rotating, it drives the threaded sleeve 4 to move longitudinally along the external thread on the surface of the threaded rod 3. The threaded sleeve 4, in cooperation with the connecting column, drives the support plate 5 to move, which in turn drives the sealing plate 7 to move. This allows the support plate 5 to extend and retract inside the fluorescence quantitative detector 1. When the support plate 5 is on the outside, multiple containers containing samples can be placed sequentially on top of the rectangular limiting groove 6, achieving simultaneous detection of multiple samples and greatly improving detection efficiency. After completion, the reverse operation can reset the carrier plate 5. Since the device is driven by the motor 2, it can achieve uniform speed movement, preventing manual pulling of the carrier plate 5 from causing the container on top of the carrier plate 5 to tip over due to the inability to control the force. This improves the stability during transportation. Then, the electric telescopic rod 9 is activated, which drives the connecting plate 10 to move downwards. The connecting plate 10 drives the probe 11 to move downwards until the probe 11 extends into the container. The probe 11 releases a fluorescent signal, and the signal intensity is proportional to the amount of PCR product. Based on this, the methylation degree of the sample can be calculated and displayed on the display screen 14 for easy viewing and recording by the user.

Claims

1. A quantitative fluorescence detection device for methylated DNA, characterized in that: The device includes a fluorescence quantitative detector (1), a motor (2) is fixedly installed at the rear end of the right side of the fluorescence quantitative detector (1), a threaded rod (3) is fixedly connected to the output end of the motor (2), a threaded sleeve (4) is threadedly connected to the surface of the threaded rod (3), a bearing plate (5) is fixedly connected to the top of the threaded sleeve (4) through a connecting column, a rectangular limiting groove (6) is provided on both sides of the top of the bearing plate (5), a sealing plate (7) is provided on the right side of the rectangular limiting groove (6) and outside the fluorescence quantitative detector (1), an electric telescopic rod (9) is fixedly connected to the top of the fluorescence quantitative detector (1), a connecting plate (10) is fixedly connected to the bottom of the electric telescopic rod (9), and a set of probes (11) is provided on both sides of the bottom of the connecting plate (10).

2. The quantitative fluorescence detection device for methylated DNA according to claim 1, characterized in that: The fluorescence quantitative detector (1) has heat dissipation vents on both the front and rear sides, and the inner cavity of the heat dissipation vents is equipped with a dustproof net.

3. The quantitative fluorescence detection device for methylated DNA according to claim 1, characterized in that: A sealing ring (8) is bonded around the edge of the sealing plate (7), and the sealing ring (8) is made of rubber.

4. The quantitative fluorescence detection device for methylated DNA according to claim 1, characterized in that: A power supply box (12) is fixedly connected to the rear end of the right side of the fluorescence quantitative detector (1) and above the motor (2). The inner cavity of the power supply box (12) is equipped with a storage battery (13).

5. The quantitative fluorescence detection device for methylated DNA according to claim 1, characterized in that: One end of the threaded rod (3) is movably connected to a bearing, and one side of the bearing is fixedly connected to one side of the inner cavity of the fluorescence quantitative detector (1).

6. The quantitative fluorescence detection device for methylated DNA according to claim 1, characterized in that: The bottom of the inner cavity of the fluorescence quantitative detector (1) is provided with a sliding groove, and the bottom of the threaded sleeve (4) is fixedly connected to a slider, and the bottom of the slider is slidably connected to the inner wall of the sliding groove.

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