Cell culture dish

Abstract

The utility model discloses a cell culture dish, and relates to the technical field of cell culture equipment, the cell culture dish comprises a dish body and a cover body, the cell culture dish is characterized in that the cover body is buckled on the dish body, and the outer side of the side wall of the dish body is sleeved with the side wall of the cover body; a plurality of circumferentially and uniformly distributed extension clamping strips are arranged on the outer side of an upper edge opening of the dish body, a plurality of first air holes for ventilating the interior of the dish body are formed in the side wall of the dish body, and the first air holes are all located below the extension clamping strips; a plurality of inward extending clamping strips are arranged on the inner side of the side wall of the cover body, the outward extending clamping strips are located between the inward extending clamping strips and the top wall of the cover body and used for clamping the cover body and the dish body, a plurality of second air holes are formed in the side wall of the cover body, and a sealing strip is arranged on the inner side of the side wall of the cover body; the sealing strip is provided with a plurality of third air holes aligned with the second air holes. According to the device, the air tightness in the cell culture dish can be conveniently controlled, and the device can be suitable for culture of different cells.

Description

Technical Field

[0001] This application relates to the field of cell culture equipment technology, and in particular to a cell culture dish. Background Technology

[0002] A cell culture dish is a laboratory vessel used for culturing microorganisms or cells. It consists of a flat, disc-shaped base and a lid, and is generally made of glass or plastic. Culture dishes are basically divided into two categories: plastic and glass. Glass dishes can be used for the culture of plant materials, microorganisms, and adherent cultures of animal cells. Plastic dishes, often made of polyethylene, are available in disposable and reusable versions, suitable for laboratory inoculation, streaking, and bacterial isolation procedures, and can be used for the culture of plant materials.

[0003] For example, the cell culture dish with patent number CN201620806578.0 has the following shortcomings in actual use:

[0004] The aforementioned cell culture dish includes a culture body and a culture cap for covering the culture body. The culture body includes an integrally connected upper side wall, a lower side wall, a flange on the outer surface of the junction of the upper and lower side walls, and a circular bottom wall. The culture cap includes an integrally connected side wall and a circular top wall. The culture body and the culture cap are directly fastened together, making it difficult for operators to control the air permeability or sealing state inside the cell culture dish, and making it difficult to adapt to the culture of different cells. Summary of the Invention

[0005] To address the technical problem of inconvenient air permeability control in cell culture dishes, this application provides a cell culture dish.

[0006] The cell culture dish provided in this application adopts the following technical solution:

[0007] A cell culture dish includes a dish body and a lid, wherein the lid is fastened to the dish body and the sidewall of the lid is fitted onto the outside of the sidewall of the dish body.

[0008] The outer side of the upper edge of the dish is provided with several circumferentially distributed outer strips, and the side wall of the dish is provided with several first vent holes for ventilation inside the dish. The first vent holes are all located below the outer strips.

[0009] The inner side wall of the cover is provided with several inner extension strips, and the outer extension strip is located between the inner extension strips and the top wall of the cover for locking between the cover and the dish. Several second vent holes are provided on the side wall of the cover, and the second vent holes are aligned with the first vent holes at the corresponding positions. A sealing strip is provided on the inner side wall of the cover.

[0010] The sealing strip has several third vent holes aligned with the second vent hole.

[0011] By adopting the above technical solution, the dish body and the cover body can be interlocked with each other, and the opening at the top of the dish body is sealed by the cover body. The dish body and the cover body can be interlocked by the outer and inner extension strips, and there is relative rotation space when they are interlocked. The alignment of the first vent hole and the second vent hole can be controlled according to the relative rotation position, thereby controlling the ventilation or sealing inside the cell culture dish. Moreover, the sealing strip can further improve the sealing effect.

[0012] Preferably, a stabilizing groove is provided on the top of the cover, and the diameter of the stabilizing groove is the same as the outer diameter of the bottom of the dish. Several magnetic blocks are provided at the bottom of the stabilizing groove, and several iron pieces are embedded in the bottom of the dish.

[0013] By adopting the above technical solution, when multiple cell culture dishes are stacked, the upper dish is located exactly in the stabilizing groove, and the stability of the stacking is ensured by the attraction between the magnetic block and the iron plate.

[0014] Preferably, the bottom of the stabilizing groove is provided with a plurality of strip-shaped slots, the magnetic block is located inside the strip-shaped slots, the opening of the strip-shaped slot is provided with an inner protrusion for holding the magnetic block, and the end of the strip-shaped slot away from the magnetic block is provided with a pick-and-place hole, the width of the pick-and-place hole is the same as the width of the strip-shaped slot, so that the magnetic block can be picked up and placed.

[0015] By adopting the above technical solution, the magnetic block can be taken out and put in through the pick-and-place hole. When the cells cultured inside the dish have a strong sensitivity to the magnetic field, the magnetic block can be taken out to prevent the magnetic field of the magnetic block from affecting the cell culture experiment.

[0016] Preferably, one end of the inner extension strip is provided with a stop.

[0017] By adopting the above technical solution, the stop block can limit the relative position of the outer and inner locking strips when the dish body and the lid body rotate and lock together, thus preventing excessive rotation.

[0018] Preferably, two positioning protrusions are provided above the end of the inner extension strip near the stop block, and two grooves matching the positioning protrusions are provided at one end of the outer extension strip.

[0019] By adopting the above technical solution, when the cover and the dish are rotated and locked together, the inner and outer locking strips are first separated from each other, and the cover is placed on the dish. By rotating the cover, the outer and inner locking strips gradually overlap in the vertical direction. After rotating to a certain angle, one of the positioning protrusions overlaps with the groove. At this time, the first vent hole and the second vent hole are separated, and the cell culture dish is in a sealed state. By continuing to rotate the cover, the two positioning protrusions can overlap with the two grooves respectively. At this time, the first vent hole and the second vent hole are aligned, and the cell culture dish is in a breathable state. This makes it easier to determine the rotation position of the cover in the sealed and breathable states, and is more convenient to use.

[0020] Preferably, the outer edge of the card strip has a first chamfer at the end near the groove, and the inner edge of the card strip has a second chamfer at the end away from the positioning protrusion.

[0021] By adopting the above technical solution, when the outer and inner card strips are connected, the first and second chamfers will make contact first, which will guide the outer and inner card strips and improve the smoothness of operation.

[0022] Preferably, the outer side of the sealing strip is integrally formed with a plurality of mushroom-shaped nails, and the side wall of the cover body is provided with a plurality of mounting holes that match the mushroom-shaped nails. The sealing strip is fixed to the inner side of the cover body through the mushroom-shaped nails and the mounting holes.

[0023] By adopting the above technical solution, the sealing strip is fixed to the inside of the lid by mushroom nails, which makes it easy for operators to disassemble and replace the aging or worn sealing strip, thereby improving the service life of the cell culture dish.

[0024] Preferably, the cover and the dish are made of borosilicate glass.

[0025] By adopting the above technical solution, the high borosilicate glass material is not only high in hardness, but also a high-transparency material, making it easier for operators to observe the experimental conditions inside the cell culture dish.

[0026] In summary, this application includes at least one of the following beneficial technical effects:

[0027] 1. The cover and the dish body are interlocked by outer and inner locking strips and can rotate relative to each other. Rotating the cover aligns the first and second vent holes, allowing air to pass through the cell culture dish. Conversely, rotating the cover separates the first and second vent holes, sealing the inside of the cell culture dish. This facilitates control over the airtightness of the cell culture dish and is suitable for culturing different types of cells.

[0028] 2. The sealing strip can improve the sealing between the cover and the dish. When the first vent and the second vent are separated, it prevents the exchange of gas between the inside and outside of the cell culture dish through the first vent, the second vent, and the gap between the cover and the dish, thus improving the sealing effect of the cell culture dish. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the overall isometric structure of this application;

[0030] Figure 2 This is a schematic diagram of the bottom structure of this application;

[0031] Figure 3 This is a schematic diagram of the structure in this application where the cover and the dish are separated;

[0032] Figure 4 For this application Figure 3 Enlarged structural diagram at point A;

[0033] Figure 5 This is a schematic diagram of the inner structure of the cover in this application;

[0034] Figure 6 This is a schematic diagram showing the disassembled structure of the sealing strip in this application.

[0035] Reference numerals: 1. Dish body; 11. Outer retaining strip; 12. First vent hole; 13. Groove; 14. First chamfer;

[0036] 2. Cover; 21. Inner extension retaining strip; 22. Second vent hole; 23. Stabilizing groove; 24. Strip-shaped retaining groove; 25. Removal and placement hole; 26. Stop block; 27. Positioning protrusion; 28. Second chamfer; 29. ​​Mounting hole;

[0037] 3. Sealing strip; 31. Third vent; 32. Mushroom-shaped nail;

[0038] 4. Magnetic block; 5. Iron sheet. Detailed Implementation

[0039] The following is in conjunction with the appendix Figure 1-6 This application will be described in further detail.

[0040] This application discloses a cell culture dish.

[0041] Reference Figure 1 , 34, 5, a cell culture dish, comprising a dish body 1 and a lid 2 that interlock with each other, wherein both dish body 1 and lid 2 are made of high borosilicate glass, which has the characteristics of high hardness and good transparency. The outer edge of the upper rim of dish body 1 is integrally formed with three circumferentially distributed outer extension strips 11, one end of which is provided with a first chamfer 14. The inner side of lid 2 is integrally formed with three inner extension strips 21, which are locked below the corresponding outer extension strips 11. One end of inner extension strip 21 is provided with a second chamfer 28. During the rotation and locking process, the first chamfer 14 first contacts the second chamfer 28, which plays a guiding role and makes the installation of lid 2 smoother. One end of inner extension strip 21 is provided with a stop 26, which allows the lid 2 and dish body 1 to be rotated and locked together. The stop 26 can prevent lid 2 from rotating too much, further facilitating the installation of lid 2.

[0042] With the above settings, when the cover 2 is closed, the inner extension strip 21 and the outer extension strip 11 are first separated, and the cover 2 is placed on the dish 1. Then, the cover 2 is rotated so that the inner extension strip 21 rotates to the bottom of the outer extension strip 11, thereby achieving the engagement of the cover 2 and the dish 1. Moreover, during the movement of the inner extension strip 21, the first chamfer 14 and the second chamfer 28 first come into contact, playing a guiding role and improving the smoothness of the closing. After the cover is closed, the stop block 26 will block the rotation of the cover 2, preventing the operator from rotating it excessively. When using cell culture dishes for cell culture, the highly transparent and hard borosilicate glass material makes it easier for operators to observe.

[0043] Reference Figure 1 , 2 4, 5, 6. Several first vent holes 12 for ventilation inside the vessel 1 are provided on the side wall of the vessel 1. The first vent holes 12 are all located below the outer extension strip 11. Several second vent holes 22 are provided on the side wall of the cover 2. The second vent holes 22 are aligned with the corresponding first vent holes 12. A sealing strip 3 is provided on the inner side of the side wall of the cover 2. Several mushroom nails 32 are integrally formed on the outer side of the sealing strip 3. Several mounting holes 29 matching the mushroom nails 32 are provided on the side wall of the cover 2. The sealing strip 3 is fixed to the inner side of the cover 2 by the mushroom nails 32 and the mounting holes 29. Several third vent holes 31 aligned with the second vent holes 22 are provided on the sealing strip 3.

[0044] With the above settings, when the cover 2 is rotated to engage, one of the positioning protrusions 27 first aligns with the groove 13. At this time, the first vent 12 and the second vent 22 are separated, and the cell culture dish is in a sealed state. Continuing to rotate the cover 2, the two positioning protrusions 27 will align with the two grooves 13 respectively. At this time, the first vent 12 and the second vent 22 are aligned, and the cell culture dish is in a breathable state. The sealing strip 3 can improve the sealing effect. Moreover, the sealing strip 3 is fixed to the inside of the cover 2 by the mushroom nail 32, which can be easily installed and removed. When the sealing strip 3 ages, it is more convenient to replace it.

[0045] Reference Figure 1 , 2 6. A stabilizing groove 23 is provided on the top of the cover 2, and the diameter of the stabilizing groove 23 is the same as the diameter of the bottom outer side of the dish body 1. When the cell culture dishes are stacked, the bottom of the upper cell culture dish is stably placed in the stabilizing groove 23. Several magnetic blocks 4 are provided at the bottom of the stabilizing groove 23, and several iron pieces 5 are embedded at the bottom of the dish body 1. When stacked, the magnetic blocks 4 on the lower cell culture dish and the iron pieces 5 at the bottom of the upper cell culture dish will have an adsorption effect. Several strip-shaped slots 24 are opened at the bottom of the stabilizing groove 23. The magnetic blocks 4 are located inside the strip-shaped slots 24. The opening of the strip-shaped slots 24 is provided with an inner protrusion for holding the magnetic blocks 4. The end of the strip-shaped slots 24 away from the magnetic blocks 4 is provided with a pick-and-place hole 25, and the width of the pick-and-place hole 25 is the same as the width of the strip-shaped slots 24, so that the magnetic blocks 4 can be picked up and placed.

[0046] With the above setup, when stacking cell culture dishes, the bottom of the upper cell culture dish is precisely locked in the stabilizing groove 23 of the lower cell culture dish. The adsorption between the magnetic block 4 and the iron plate 5 makes the stacking of cell culture dishes more stable. Moreover, the magnetic block 4 can slide to the pick-up hole 25 and be taken out. When the cells cultured inside the dish 1 have a strong induction of the magnetic field, the magnetic field of the magnetic block 4 is prevented from affecting the experimental data and results of the cell culture experiment.

[0047] The implementation principle of a cell culture dish according to an embodiment of this application is as follows: When the cover 2 is closed, the inner extension strip 21 and the outer extension strip 11 are first separated, and the cover 2 is placed on the dish body 1. Then, the cover 2 is rotated so that the inner extension strip 21 rotates to the bottom of the outer extension strip 11, realizing the engagement of the cover 2 and the dish body 1. During the movement of the inner extension strip 21, the first chamfer 14 and the second chamfer 28 first come into contact, playing a guiding role. Depending on the rotation angle, one of the positioning protrusions 27 coincides with the groove 13. At this time, the first vent 12 and the second vent 22 are separated, and the cell culture dish is in a sealed state. Continuing to rotate the cover 2, the two positioning protrusions 27 will coincide with the two grooves 13 respectively. At this time, the first vent 12... Aligned with the second vent 22, the cell culture dish is in a breathable state. After being rotated to a certain angle, the stop block 26 will block the rotation of the cover 2 to prevent the operator from rotating it excessively. When using cell culture dishes for cell culture, the highly transparent and hard borosilicate glass material makes it easier for the operator to observe. In addition, when stacking cell culture dishes, the bottom of the upper cell culture dish is precisely stuck in the stabilizing groove 23 of the lower cell culture dish. Through the adsorption between the magnetic block 4 and the iron plate 5, the stacking of cell culture dishes is more stable. Moreover, the magnetic block 4 can slide to the pick-up hole 25 and be taken out. When the cells cultured inside the dish 1 have a strong induction of the magnetic field, the magnetic field of the magnetic block 4 is prevented from affecting the experimental data and results of the cell culture experiment.

[0048] In summary, this device can switch between aeration and sealing states inside the cell culture dish by rotating the cover 2, making it suitable for the culture of different cells. Moreover, when stacked, the magnetic block 4 and the iron plate 5 generate an attractive force, which improves the stacking stability.

[0049] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cell culture dish, comprising a dish body (1) and a cover body (2), characterized in that: The cover (2) is fastened to the dish (1), and the side wall of the cover (2) is fitted onto the outside of the side wall of the dish (1); The outer side of the upper edge of the dish body (1) is provided with several circumferentially distributed outer extension strips (11), and the side wall of the dish body (1) is provided with several first ventilation holes (12) for ventilation inside the dish body (1), and the first ventilation holes (12) are all located below the outer extension strips (11). The inner side wall of the cover (2) is provided with several inner extension strips (21), and the outer extension strip (11) is located between the inner extension strips (21) and the top wall of the cover (2) for the snapping between the cover (2) and the dish (1). Several second vent holes (22) are opened on the side wall of the cover (2), and the second vent holes (22) are aligned with the first vent holes (12) at the corresponding positions. A sealing strip (3) is provided on the inner side wall of the cover (2). The sealing strip (3) has a plurality of third vent holes (31) aligned with the second vent hole (22).

2. The cell culture dish according to claim 1, characterized in that: A stabilizing groove (23) is provided on the top of the cover (2), and the diameter of the stabilizing groove (23) is the same as the outer diameter of the bottom of the dish (1). Several magnetic blocks (4) are provided at the bottom of the stabilizing groove (23), and several iron pieces (5) are embedded at the bottom of the dish (1).

3. A cell culture dish according to claim 2, characterized in that: The bottom of the stabilizing groove (23) is provided with several strip-shaped slots (24), and the magnetic block (4) is located inside the strip-shaped slots (24). The opening of the strip-shaped slots (24) is provided with an inner protruding edge for holding the magnetic block (4), and the end of the strip-shaped slots (24) away from the magnetic block (4) is provided with a pick-and-place hole (25), and the width of the pick-and-place hole (25) is the same as the width of the strip-shaped slots (24), so that the magnetic block (4) can be picked up and placed.

4. A cell culture dish according to claim 1, characterized in that: One end of the inner extension strip (21) is provided with a stop (26).

5. A cell culture dish according to claim 4, characterized in that: The inner extension strip (21) has two positioning protrusions (27) above the end near the stop block (26), and the outer extension strip (11) has two grooves (13) that match the positioning protrusions (27) at one end.

6. A cell culture dish according to claim 5, characterized in that: The outer extension strip (11) has a first chamfer (14) at the end near the groove (13), and the inner extension strip (21) has a second chamfer (28) at the end away from the positioning protrusion (27).

7. A cell culture dish according to claim 1, characterized in that: The outer side of the sealing strip (3) is integrally formed with a number of mushroom nails (32), and the side wall of the cover (2) is provided with a number of mounting holes (29) that match the mushroom nails (32). The sealing strip (3) is fixed to the inner side of the cover (2) by the mushroom nails (32) and the mounting holes (29).

8. A cell culture dish according to claim 1, characterized in that: The cover (2) and the dish (1) are made of borosilicate glass.

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