Vessel device for cell culture and growth

Abstract

The utility model relates to the technical field of cell culture, in particular to a vessel device for cell culture growth, which comprises a shell, a synchronous driving mechanism and a plurality of shaking mechanisms are mounted in the shell, and the shaking mechanisms are respectively connected to four sides of the synchronous driving mechanism. A transparent cover plate is movably arranged at the position, corresponding to the multiple shaking mechanisms, of the shell, and a liquid injection assembly is fixedly arranged on the transparent cover plate. The synchronous driving mechanism comprises a fixing frame fixedly arranged in the shell, a motor is installed on the fixing frame, a driving gear is fixedly arranged on an output shaft of the motor, the driving gear is meshed with a synchronous gear, and the synchronous gear is rotationally arranged on the fixing frame; according to the vessel device for cell culture and growth, by increasing the number of the culture dishes, the culture efficiency is improved, culture solutions in the culture dishes can be uniformly distributed, cells are not prone to gathering or forming blocks in the culture process, and cell proliferation and differentiation are facilitated.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a vessel device for cell culture and growth. Background Technology

[0002] Cell culture apparatus is a crucial tool in biomedical research and drug development. With the rapid development of biotechnology, the demand for cell culture technology is increasing daily. Researchers need efficient culture systems to support cell growth, proliferation, and various biological experiments. Cell culture requires apparatus to ensure cells can survive in a suitable temperature and humidity environment. However, existing apparatus has the following drawbacks: 1. Only one culture dish can be placed in each apparatus, resulting in low culture efficiency; 2. During culture, the culture dish remains stationary, leading to uneven distribution of the culture medium, which affects cell growth and proliferation. Furthermore, suspended cells tend to settle to the bottom of the dish in a static state, causing cell aggregation or clumping, which is detrimental to cell proliferation and differentiation; 3. When adding culture medium, the transparent cover needs to be completely opened, directly exposing the culture dish to air and increasing the risk of contamination by other pathogens. Utility Model Content

[0003] To address the shortcomings of existing technologies, this invention provides a vessel device for cell culture and growth, which solves the technical problems of low culture efficiency, uneven distribution of culture medium, and easy exposure of the culture dish to air.

[0004] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a vessel device for cell culture and growth, comprising a shell, wherein a synchronous drive mechanism and a plurality of shaking mechanisms are installed inside the shell, the plurality of shaking mechanisms are respectively connected to the four sides of the synchronous drive mechanism, and a transparent cover plate is movably disposed on the shell corresponding to the positions of the plurality of shaking mechanisms, and a liquid injection assembly is fixed on the transparent cover plate.

[0005] The synchronous drive mechanism includes a fixed frame fixed inside the housing, a motor mounted on the fixed frame, a drive gear fixed on the output shaft of the motor, the drive gear meshing with a synchronous gear, the synchronous gear rotatably mounted on the fixed frame, and multiple control components mounted on the fixed frame meshing on the outer side of the synchronous gear.

[0006] Preferably, the control component includes a spline sleeve rotatably connected to the bottom of the fixed frame, a spline rod is fitted inside the spline sleeve, the top of the spline rod extends to the outside of the housing, a through hole is opened on the outer wall of the spline rod, two movable blocks are movably arranged in the through hole, a spring is connected between the two movable blocks, one end of the movable block is engaged in a tooth groove opened on the inner wall of the driven gear, the driven gear meshes with the synchronous gear and is rotatably connected to the fixed frame, a swing rod is also fixed on the outer wall of the spline rod, and a push block is fixed on one end of the swing rod.

[0007] Preferably, one end of the movable block is hemispherical.

[0008] Preferably, the rocking mechanism includes a rocking platform disposed inside the housing, two sliders are fixedly disposed at the bottom of the rocking platform, the two sliders are respectively slidably disposed on two slide rails, and both slide rails are installed inside the housing. A fixing block is also fixedly disposed on the side wall of the rocking platform, and a movable hole is opened on the fixing block, and a push block is inserted into the movable hole on the fixing block.

[0009] Preferably, temperature and humidity probes are installed inside the housing at positions corresponding to the multiple shaking mechanisms.

[0010] Preferably, the injection assembly includes an injection tube fixed to a transparent cover plate, and a piston is movably connected inside the injection tube via a spring, with an injection hole inside the piston.

[0011] By employing the above technical solution, this utility model provides a vessel device for cell culture and growth, which has at least the following beneficial effects:

[0012] 1. This apparatus for cell culture and growth has an outer shell that can hold multiple culture dishes. By increasing the number of culture dishes, the culture efficiency can be improved. The synchronous drive mechanism can simultaneously drive multiple shaking mechanisms to shake, which can make the culture medium in multiple culture dishes evenly distributed. During the culture process, cells are less likely to aggregate or form clumps, which is conducive to cell proliferation and differentiation.

[0013] 2. The cell culture and growth apparatus has a synchronous drive mechanism that can independently control one of the shaking mechanisms to stop working without affecting the continued operation of the other shaking mechanisms. The advantage of this design is that one of the culture dishes can be replaced individually during use without stopping the machine.

[0014] 3. This cell culture and growth apparatus, by setting up a liquid injection component, can reduce the amount of air entering the outer shell when injecting culture medium, thereby avoiding the entire culture dish being exposed to the air and greatly improving the cell culture survival rate. Attached Figure Description

[0015] The accompanying drawings, which are included to provide a further understanding of the present invention, form part of this application:

[0016] Figure 1 This is a three-dimensional structural diagram of the entire utility model;

[0017] Figure 2 This is a partial cross-sectional view of the interior of the outer shell of this utility model;

[0018] Figure 3 This is a schematic diagram of the synchronous drive mechanism of this utility model;

[0019] Figure 4 This is a structural diagram showing the disassembled control component of this utility model;

[0020] Figure 5 This is a schematic diagram of the rocking mechanism of this utility model;

[0021] Figure 6 This is a cross-sectional structural schematic diagram of the liquid injection assembly of this utility model;

[0022] Figure 7 This utility model Figure 4 Enlarged structural diagram at point A in the middle.

[0023] Figure label:

[0024] 1. Housing; 2. Synchronous drive mechanism; 201. Fixing frame; 202. Motor; 203. Drive gear; 204. Synchronous gear; 205. Control component; 2051. Spline sleeve; 2052. Spline rod; 2053. Swing rod; 2054. Push block; 2055. Spring 1; 2056. Movable block; 2057. Driven gear; 3. Shaking mechanism; 301. Shaking table; 302. Slider; 303. Slide rail; 304. Fixing block; 4. Temperature and humidity probe; 5. Transparent cover plate; 6. Liquid injection component; 601. Liquid injection tube; 602. Spring 2; 603. Piston; 604. Liquid injection hole. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Cell culture apparatus is a device used to cultivate and observe cells in a laboratory environment, primarily for research in fields such as biology, medicine, and biotechnology. Its basic function is to provide the environment necessary for cell growth, including nutrients, suitable temperature, pH, and gas composition (such as oxygen and carbon dioxide).

[0027] Example 1:

[0028] Due to the limitations of existing technologies, such as low cultivation efficiency, uneven distribution of the culture medium, and easy exposure of the culture dish to air, please refer to... Figures 1-7 This utility model provides a vessel device for cell culture and growth, which can improve culture efficiency and ensure uniform distribution of culture medium in multiple culture dishes. During the culture process, cells are less likely to aggregate or form clumps, which is beneficial to cell proliferation and differentiation. The device includes an outer shell 1, inside which a synchronous drive mechanism 2 and multiple shaking mechanisms 3 are installed. The multiple shaking mechanisms 3 are respectively connected to the four sides of the synchronous drive mechanism 2. A transparent cover plate 5 is also movably disposed on the outer shell 1 corresponding to the positions of the multiple shaking mechanisms 3, and a liquid injection component 6 is fixed on the transparent cover plate 5. Multiple culture dishes are placed in the outer shell 1, and then the synchronous drive mechanism 2 is used to move the multiple shaking mechanisms 3, thereby causing the culture dishes to shake.

[0029] To solve the problem of simultaneously shaking multiple petri dishes, please refer to... Figure 3 The synchronous drive mechanism 2 includes a fixed frame 201 fixed inside the outer casing 1. A motor 202 is mounted on the fixed frame 201. A drive gear 203 is fixed on the output shaft of the motor 202. The drive gear 203 meshes with a synchronous gear 204. The synchronous gear 204 is rotatably mounted on the fixed frame 201. Multiple control components 205 mounted on the fixed frame 201 mesh on the outer side of the synchronous gear 204. The motor 202 drives the drive gear 203 to rotate, and the drive gear 203 drives the synchronous gear 204 to rotate. Under the action of the control components 205, the synchronous gear 204 can drive the shaking mechanism 3 to move. Only by controlling the forward and reverse rotation of the motor 202, the petri dish can be shaken.

[0030] For easy handling of the petri dish, please refer to... Figure 4The control component 205 includes a spline sleeve 2051 rotatably connected to the bottom of the fixed frame 201. A spline rod 2052 is fitted inside the spline sleeve 2051. The top of the spline rod 2052 extends to the outside of the outer casing 1. A through hole is formed on the outer wall of the spline rod 2052. Two movable blocks 2056 are movably disposed in the through hole. A spring 2055 is connected between the two movable blocks 2056. One end of the movable block 2056 is engaged in a toothed groove formed on the inner wall of the driven gear 2057. The driven gear 2057 meshes with the synchronous gear 204 and is rotatably connected to the fixed frame 201. The outer wall of the spline rod 2052... A swing rod 2053 is also fixedly installed on the upper part, and a push block 2054 is fixedly installed on one end of the swing rod 2053. When it is necessary to pick up or put down one of the culture dishes, the spline rod 2052 is pulled upward. When the movable block 2056 disengages from the driven gear 2057, under the action of the spring force 2055, the movable block 2056 can move outward. At this time, the rotation of the driven gear 2057 will not drive the spline rod 2052 to rotate, so that the shaking mechanism 3 will not move. The shaking mechanism 3 is in a stationary state at this time, so that the culture dishes can be picked up or put down. The other shaking mechanisms 3 are still in motion and will not affect the other culture dishes.

[0031] To facilitate the insertion of the movable block 2056 into the driven gear 2057, one end of the movable block 2056 is made hemispherical.

[0032] To shake the culture dish, please refer to... Figure 5 The shaking mechanism 3 includes a shaking table 301 disposed inside the housing 1. Two sliders 302 are fixedly disposed at the bottom of the shaking table 301. The two sliders 302 are slidably disposed on two slide rails 303 respectively, and both slide rails 303 are installed inside the housing 1. A fixing block 304 is also fixedly disposed on the side wall of the shaking table 301. The fixing block 304 has a movable hole. A push block 2054 is inserted into the movable hole on the fixing block 304. Based on the movement of the control component 205, when the movable block 2056 is locked in the driven gear 2057, the driven gear 2057 rotates, which drives the spline rod 2052 to rotate. The spline rod 2052 drives the swing rod 2053 to rotate. The swing rod 2053, through the connection between the push block 2054 and the fixing block 304, causes the shaking table 301 to shake in the left and right directions, thereby shaking the petri dish.

[0033] To monitor the temperature and humidity of the culture in real time, temperature and humidity probes 4 are installed inside the outer shell 1 at positions corresponding to multiple shaking mechanisms 3.

[0034] Example 2:

[0035] When it is necessary to add culture medium to a petri dish, the existing technology involves directly opening the transparent cover 5 and then injecting the culture medium into the petri dish through a syringe. However, after opening the transparent cover 5, the entire petri dish is directly exposed to the air, making it susceptible to contamination by other pathogens. To solve this problem, please refer to... Figure 6 The liquid injection assembly 6 includes a liquid injection tube 601 fixed on a transparent cover plate 5. A piston 603 is movably connected inside the liquid injection tube 601 via a spring 602. A liquid injection hole 604 is opened inside the piston 603. The head of the needle is inserted into the liquid injection tube 601 and squeezes the piston 603. The piston 603 moves down and exposes the liquid injection hole 604. At this time, squeezing the needle can inject the culture medium into the culture dish through the liquid injection hole 604. After the injection is completed, the needle is removed, and the piston 603 is reset under the pulling force of the spring 602, thereby sealing the liquid injection hole 604, thus solving the problem of the entire culture dish being exposed to the air when adding culture medium.

[0036] As can be seen from the above embodiments: multiple petri dishes are placed inside the outer shell 1, and then the motor 202 drives the drive gear 203 to rotate. The drive gear 203 drives the synchronous gear 204 to rotate, and the synchronous gear 204 drives the driven gear 2057 to rotate. When the movable block 2056 is stuck in the driven gear 2057, the rotation of the driven gear 2057 drives the spline rod 2052 to rotate. The spline rod 2052 drives the swing rod 2053 to rotate. The swing rod 2053, through the connection between the push block 2054 and the fixed block 304, causes the rocking table 301 to rock left and right, thereby shaking the petri dishes. After the culture is completed, the transparent cover 5 is opened and the petri dishes are taken out.

[0037] It should be noted that the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A vessel for cell culture and growth, comprising a shell (1), characterized in that: The outer shell (1) is equipped with a synchronous drive mechanism (2) and multiple shaking mechanisms (3). The multiple shaking mechanisms (3) are respectively connected to the four sides of the synchronous drive mechanism (2). A transparent cover plate (5) is also movably arranged on the outer shell (1) at the position corresponding to the multiple shaking mechanisms (3). An injection assembly (6) is fixed on the transparent cover plate (5). The synchronous drive mechanism (2) includes a fixed frame (201) fixed inside the housing (1), a motor (202) is mounted on the fixed frame (201), a drive gear (203) is fixed on the output shaft of the motor (202), the drive gear (203) meshes with a synchronous gear (204), the synchronous gear (204) is rotatably mounted on the fixed frame (201), and a plurality of control components (205) mounted on the fixed frame (201) mesh on the outer side of the synchronous gear (204).

2. The apparatus for cell culture and growth according to claim 1, characterized in that: The control component (205) includes a spline sleeve (2051) rotatably connected to the bottom of the fixed frame (201). A spline rod (2052) is fitted inside the spline sleeve (2051). The top of the spline rod (2052) extends to the outside of the outer shell (1). A through hole is opened on the outer wall of the spline rod (2052). Two movable blocks (2056) are movably arranged in the through hole. A spring (2055) is connected between the two movable blocks (2056). One end of the movable block (2056) is engaged in a tooth groove opened on the inner wall of the driven gear (2057). The driven gear (2057) meshes with the synchronous gear (204) and is rotatably connected to the fixed frame (201). A swing rod (2053) is also fixed on the outer wall of the spline rod (2052). A push block (2054) is fixed on one end of the swing rod (2053).

3. The apparatus for cell culture and growth according to claim 2, characterized in that: One end of the movable block (2056) is hemispherical.

4. The apparatus for cell culture and growth according to claim 2, characterized in that: The rocking mechanism (3) includes a rocking platform (301) disposed inside the housing (1). Two sliders (302) are fixedly disposed at the bottom of the rocking platform (301). The two sliders (302) are slidably disposed on two slide rails (303), and both slide rails (303) are installed inside the housing (1). A fixing block (304) is also fixedly disposed on the side wall of the rocking platform (301). A movable hole is provided on the fixing block (304), and a push block (2054) is inserted into the movable hole on the fixing block (304).

5. The apparatus for cell culture and growth according to claim 1, characterized in that: Temperature and humidity probes (4) are installed inside the outer casing (1) at positions corresponding to multiple shaking mechanisms (3).

6. The apparatus for cell culture and growth according to claim 1, characterized in that: The liquid injection assembly (6) includes a liquid injection tube (601) fixed on a transparent cover plate (5). A piston (603) is movably connected inside the liquid injection tube (601) via a spring (602). A liquid injection hole (604) is opened inside the piston (603).

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