In-vitro organoid culture device

Abstract

The utility model discloses an in vitro organoid culture device, relates to cell culture device technical field, including culture device body and sliding connection in the inner side of culture device body, culture device body is provided with the pressing mechanism, the pressing mechanism can improve the air tightness of drawing plate and culture device body, and the culture device body is more compact. The pressing mechanism comprises a first sealing ring fixedly connected to the interior of the culture device body, placing boxes are fixedly connected to the left side and the right side of the culture device body, and sliding columns are slidably connected to the interiors of the placing boxes. Through the arrangement of a rotary knob, a first sealing ring, a second sealing ring and a pressing block, the air tightness of the whole device can be remarkably improved, the using effect of the device is improved, and the problems that in the using process of existing culture devices, most of the existing culture devices are lack of sealing structures, and the use cost is high are solved. The problem that the working effect of the device is greatly influenced due to the fact that external air possibly enters the device in the culture process is solved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, specifically to an in vitro organoid culture device. Background Technology

[0002] Organoid culture technology is mainly divided into three-dimensional culture technology that relies on exogenous scaffolds and three-dimensional culture technology that does not rely on exogenous scaffolds. Three-dimensional culture technology that does not rely on exogenous scaffolds mainly uses physical methods to aggregate cells suspended in culture medium to a certain density to form tight cell-cell junctions, allowing them to self-assemble into organoid three-dimensional structures that do not rely on exogenous scaffolds. Currently, the main technology used in the market is the suspension culture method, but existing culture devices still encounter some problems in actual use.

[0003] For example, application number CN202221790159.4 discloses an organoid culture device, including an outer cylinder and an inner cylinder disposed inside the outer cylinder. The outer cylinder is provided with a top cover. The upper part of the inner cylinder has multiple slots around its circumference, and the slots pass through the bottom wall of the inner cylinder. The upper part of the top cover has multiple second through holes around its circumference, and the second through holes are corresponding to the slots. The inner cylinder has a liquid exchange tank that passes through it coaxially. The top cover has a first through hole that passes through it coaxially. This device is characterized by its ability to facilitate classified storage. In the process of use, most existing culture devices lack a sealing structure, and outside air may enter the device during the culture process, which greatly affects the working effect of the device.

[0004] To address the aforementioned problems, an in vitro organoid culture device is proposed. Utility Model Content

[0005] The purpose of this invention is to provide an in vitro organoid culture device. By using this device, the problem of existing culture devices lacking a sealing structure during use, which may allow outside air to enter the device during the culture process, greatly affecting the working effect of the device, is solved.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an in vitro organoid culture device, comprising a culture device body and a pull-out plate slidably connected to the inner side of the culture device body. The culture device body is provided with a pressing mechanism, which improves the airtightness between the pull-out plate and the culture device body. The pressing mechanism includes a sealing ring one fixedly connected inside the culture device body. Placement boxes are fixedly connected to both the left and right sides of the culture device body, and a sliding column is slidably connected inside the placement box. A rotating rod is rotatably connected to one end of the sliding column. A sealing ring two is fixedly connected to the inner side of the pull-out plate.

[0007] Preferably, the first sealing ring and the second sealing ring are matched, a knob is rotatably connected to one side of the placement box, and a bevel gear is fixedly connected to one side of the knob, and a bevel gear is meshed with one side of the first bevel gear.

[0008] The design of the above structure, through the setting of bevel gear one and bevel gear two, changes the transmission direction of the knob, thus advancing the workflow.

[0009] Preferably, the second bevel gear is rotatably connected inside the placement box, a lead screw is fixedly connected to one side of the second bevel gear, and the lead screw is threadedly connected to the sliding column, and a pressing block is fixedly connected to one side of the rotating rod.

[0010] The above-described structure design, through the placement box, allows the relevant components to be placed inside the box, thereby improving the space utilization of the device.

[0011] Preferably, one end of the sliding column is slidably connected to a pulling block, and one end of the pulling block is fixedly connected to a cross block, and one end of the pulling block is also fixedly connected to a spring rod.

[0012] The design of the above structure, with the spring rod, enables the pull block to automatically reset, improving the convenience of use.

[0013] Preferably, a cross groove is provided on the other side of the rotating rod, and the cross groove slides in conjunction with the cross block, and a storage bucket is fixedly connected to one side of the pull-out plate.

[0014] The design of the above structure, through the setting of the cross groove and the cross block, enables the rotating rod to be stably in a vertical or horizontal state.

[0015] Preferably, a fixed frame is fixedly connected inside the placement bucket, and a motor is also fixedly connected inside the placement bucket, with a lead screw fixedly connected to the output end of the motor.

[0016] The above-described structure, through the inclusion of a motor, enables the operator to increase the rate at which the incubator is placed.

[0017] Preferably, the outer side of the lead screw is threaded with a placement bracket, and the placement bracket is slidably connected to the outer side of the fixed bracket.

[0018] The design of the above structure, through the sliding connection of the placement rack, ensures that the placement rack will not deviate from its movement trajectory during the movement process.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] 1. The knob, sealing ring one, sealing ring two, and pressing block of this application significantly improve the airtightness of the overall device, enhance the device's performance, and solve the problem that most existing culture devices lack a sealing structure, which may allow outside air to enter the device during the culture process, greatly affecting the device's working performance.

[0021] 2. This application improves the speed at which workers can place the culture vessel by incorporating a motor, lead screw, fixing frame, and placement rack. This makes the device more convenient to use and solves the problem that most existing culture devices are placed manually, which can easily lead to contamination inside the device and is inconvenient to operate. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0023] Figure 2 This is a structural diagram of the sealing ring and the placement box of this utility model;

[0024] Figure 3 This is a structural diagram of the knob and pull block of this utility model;

[0025] Figure 4 This is a structural diagram of the cross groove and cross block of this utility model;

[0026] Figure 5 This is a structural diagram of the motor and the mounting frame of this utility model.

[0027] In the diagram: 1. Culture device body; 11. Sealing ring one; 12. Placement box; 121. Sliding column; 122. Knob; 123. Bevel gear one; 124. Bevel gear two; 125. Lead screw one; 13. Rotating rod; 131. Pressing block; 132. Pulling block; 133. Cross block; 134. Spring rod; 135. Cross groove; 2. Pull-out plate; 21. Sealing ring two; 22. Placement bucket; 221. Fixing frame; 222. Motor; 223. Lead screw two; 224. Placement rack. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] To further understand the content of this utility model, a detailed description of this utility model will be provided in conjunction with the accompanying drawings.

[0030] Combination Figures 1-4 An in vitro organoid culture device includes a culture device body 1 and a pull-out plate 2 slidably connected to the inner side of the culture device body 1. The culture device body 1 is provided with a pressing mechanism, which can improve the airtightness between the pull-out plate 2 and the culture device body 1. The pressing mechanism includes a sealing ring 11 fixedly connected to the inside of the culture device body 1. Placement boxes 12 are fixedly connected to both the left and right sides of the culture device body 1, and a sliding column 121 is slidably connected inside the placement box 12. A rotating rod 13 is rotatably connected to one end of the sliding column 121. A second sealing ring 21 is fixedly connected to the inner side of the pull-out plate 2.

[0031] The present invention will be further described below with reference to the embodiments.

[0032] Example 1:

[0033] To address the problem that most existing culture devices lack a sealing structure, allowing outside air to enter during cultivation and significantly impacting their performance, the following solution is disclosed. Please refer to the following for details. Figures 1-5Sealing ring 11 matches sealing ring 21. A knob 122 is rotatably connected to one side of the placement box 12, and a bevel gear 123 is fixedly connected to one side of the knob 122. A bevel gear 124 is meshed with one side of the bevel gear 123. The bevel gear 124 is rotatably connected inside the placement box 12. A lead screw 125 is fixedly connected to one side of the bevel gear 124, and the lead screw 125 is threadedly connected to the sliding column 121. A pressing block 131 is fixedly connected to one side of the rotating rod 13. A pulling block 132 is slidably connected to one end of the sliding column 121, and a cross block 133 is fixedly connected to one end of the pulling block 132. A spring rod 134 is also fixedly connected to one end of the pulling block 132. The other end of the rotating rod 13... A cross groove 135 is provided on the side, and the cross groove 135 slides with the cross block 133. A placement bucket 22 is fixedly connected to one side of the pull-out plate 2. A fixing frame 221 is fixedly connected inside the placement bucket 22. A motor 222 is also fixedly connected inside the placement bucket 22, and a lead screw 223 is fixedly connected to the output end of the motor 222. A placement frame 224 is threadedly connected to the outer side of the lead screw 223, and the placement frame 224 is slidably connected to the outer side of the fixing frame 221. When using this device, the incubator needs to be placed on the placement frame 224. After placement, the motor 222 can be started. The motor 222 drives the lead screw 223 to rotate, thereby causing the placement frame 224 to move outside the fixing frame 221. At this time, the placement frame 224... 4. Move the device to the inside of the placement container 22. The user can push the pull plate 2 so that the sealing ring 21 on one side of the pull plate 2 fits with the sealing ring 11 inside the culture device body 1. After placing the pull plate 2, the user can pull the pull block 132 located on one side of the sliding column 121 so that the cross block 133 disengages from the cross groove 135, bringing the rotating rod 13 to a horizontal position. Then, release the pull block 132. Under the effect of the spring rod 134, the cross block 133 automatically engages with the cross groove 135, thus fixing the rotating rod 13. Finally, turn the knob 122 located on one side of the placement box 12. The knob 122 drives the bevel gear 123 to rotate, which in turn drives the bevel gear 124 to rotate. The bevel gear 124 drives the bevel gear 124 to rotate. The rotation of the lead screw 125 causes the sliding column 121 to slide inside the placement box 12. The sliding column 121 drives the rotating rod 13 to move. At this time, the pressing block 131 on the rotating rod 13 can press the pull plate 2, making the connection between the sealing ring 21 and the sealing ring 11 tighter. When it is necessary to remove the incubator, the knob 122 can be rotated in the opposite direction to disengage the pressing block 131 from the pull plate 2. Then, the pulling block 132 is pulled to make the rotating rod 13 vertical. Finally, the pull plate 2 is pulled and the motor 222 is started, so that the placement rack 224 is moved out of the placement box 22. At this time, the removal operation can be performed, which can significantly improve the airtightness of the overall device and improve the use effect of the device.

[0034] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0035] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An in vitro organoid culture device, comprising a culture device body (1) and a pull-out plate (2) slidably connected to the inner side of the culture device body (1), characterized in that: The culture device body (1) is provided with a pressing mechanism, which can improve the airtightness between the pull plate (2) and the culture device body (1). The pressing mechanism includes a sealing ring (11) fixedly connected inside the culture device body (1). Placement boxes (12) are fixedly connected to both the left and right sides of the culture device body (1), and a sliding column (121) is slidably connected inside the placement box (12). A rotating rod (13) is rotatably connected to one side of the sliding column (121) near one end. A sealing ring (21) is fixedly connected to the inner side of the pull plate (2).

2. The in vitro organoid culture device according to claim 1, characterized in that: The sealing ring one (11) is matched with the sealing ring two (21). A knob (122) is rotatably connected to one side of the placement box (12), and a bevel gear one (123) is fixedly connected to one side of the knob (122), and a bevel gear two (124) is meshed with one side of the bevel gear one (123).

3. The in vitro organoid culture device according to claim 2, characterized in that: The second bevel gear (124) is rotatably connected inside the placement box (12). A lead screw (125) is fixedly connected to one side of the second bevel gear (124), and the lead screw (125) is threadedly connected to the sliding column (121). A pressing block (131) is fixedly connected to one side of the rotating rod (13).

4. The in vitro organoid culture device according to claim 3, characterized in that: One end of the sliding column (121) is slidably connected to a pulling block (132), and one end of the pulling block (132) is fixedly connected to a cross block (133). One end of the pulling block (132) is also fixedly connected to a spring rod (134).

5. The in vitro organoid culture device according to claim 4, characterized in that: A cross groove (135) is provided on the other side of the rotating rod (13), and the cross groove (135) slides with the cross block (133). A storage bucket (22) is fixedly connected to one side of the pull-out plate (2).

6. The in vitro organoid culture device according to claim 5, characterized in that: The placement bucket (22) is fixedly connected to a fixed frame (221), and a motor (222) is also fixedly connected to the inside of the placement bucket (22), and a lead screw (223) is fixedly connected to the output end of the motor (222).

7. The in vitro organoid culture device according to claim 6, characterized in that: The outer side of the lead screw (223) is threaded with a placement bracket (224), and the placement bracket (224) is slidably connected to the outer side of the fixed bracket (221).

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