Pathogen nucleic acid aerosol purification device

Abstract

A pathogen nucleic acid aerosol purification device comprises a machine shell, the machine shell comprises a frame, a front panel, a left side plate, a right side plate, a rear baffle and a top cover plate are arranged on the frame, the front panel is an arc-shaped plate with the plate face bent forwards gradually from bottom to top, the rear baffle and the top cover plate are rectangular, and an air inlet is formed in the bottom of the machine shell. The front panel, the left side plate, the right side plate, the rear baffle and the top cover plate define an air channel allowing air to circulate from bottom to top, and a plurality of centrifugal fans are arranged on the upper portion in the air channel side by side at intervals in the left-right horizontal direction. The utility model aims to provide a biological laboratory air purification device which can enable air in a biological laboratory to fully circulate without dead angles, enables the air to be free of eddy current and cross disturbance as far as possible during circulation, and can efficiently disinfect and remove nucleic acid fragments, viruses and bacterial microorganisms in aerosol in the air in the biological laboratory in an extremely short time. And the pathogen nucleic acid aerosol purification device is used for efficiently removing suspended particulate matters in air in a biological laboratory.

Description

Technical Field

[0001] This utility model relates to a pathogen nucleic acid aerosol purification device. Background Technology

[0002] Aerosol nucleic acid fragments are generated during the shaking and centrifugation of nucleic acid samples in biological testing experiments. These fragments readily diffuse into the air after the sample is opened, and even minute amounts can contaminate the entire laboratory. Aerosol nucleic acid fragment contamination is a common problem in laboratories, and addressing this issue is an urgent problem that needs to be solved in biological testing experiments, a challenge faced by the biological field both domestically and internationally. Utility Model Content

[0003] The purpose of this invention is to provide a pathogen nucleic acid aerosol purification device that allows for complete air circulation without dead zones in a biological laboratory, ensuring minimal eddies and cross-disturbance during air circulation, and efficiently disinfecting and removing nucleic acid fragments, viruses, and bacterial microorganisms present in aerosols in the air of a biological laboratory in a very short time, and efficiently removing suspended particulate matter present in the air of a biological laboratory.

[0004] The pathogen nucleic acid aerosol purification device of this utility model includes a housing, which includes a frame. The frame is provided with a front panel, a left side panel, a right side panel, a rear baffle and a top cover. The front panel is an arc-shaped panel that gradually bends forward from bottom to top. The rear baffle and the top cover are rectangular. The surface of the rear baffle is located in the left and right vertical direction, and the surface of the top cover is located in the horizontal direction.

[0005] The bottom of the casing is the air inlet. The front panel, left side panel, right side panel, rear baffle and top cover form an air duct that allows air to flow from bottom to top. Multiple centrifugal fans are arranged side by side at intervals along the horizontal direction in the upper part of the air duct. The air inlet of each centrifugal fan is located in the air duct. The air outlet of each centrifugal fan is connected to the air inlet of the arc-shaped jet pipe. The arc-shaped jet pipe is arranged along the front and back direction. The cross section of the arc-shaped jet pipe along the vertical direction is rectangular. The air outlet end of each arc-shaped jet pipe is fixedly connected to the upper part of the front panel. Each arc-shaped jet pipe is a curved arc with a lower air inlet end and a higher air outlet end.

[0006] An ozone removal filter is installed horizontally below the centrifugal fan in the air duct. Below the ozone removal filter, a microporous filter membrane made of H14 grade high-efficiency filter material is installed horizontally in the air duct. Below the microporous filter membrane, an air electrostatic disinfection and purification module is installed horizontally in the air duct. A coarse filter is installed at the air inlet at the bottom of the casing.

[0007] The present invention relates to a pathogen nucleic acid aerosol purification device, wherein the bottom or side of the front panel is connected to the frame by a hinge, the upper part of the front panel is connected to the frame, the left side panel, or the right side panel by screws or door latches, and an airtight strip is provided at the edge of the front panel.

[0008] The present invention relates to a pathogen nucleic acid aerosol purification device, wherein the number of centrifugal fans is 3-5, the centrifugal fans are adjustable air volume fans, the height of the casing along the vertical direction is 1500mm-1700mm, the width of the casing along the horizontal direction is 1400mm-1900mm, the width of the top of the casing along the front-back direction is 600mm-800mm, and the width of the bottom of the casing along the front-back direction is 300mm-500mm.

[0009] The present invention relates to a pathogen nucleic acid aerosol purification device, wherein the air electrostatic disinfection and purification module includes multiple foam metal plates whose surfaces can be traversed by airflow. The surfaces of the foam metal plates are arranged vertically along the front and back direction, and airflow channels are provided between the foam metal plates. A discharge electrode is provided in the middle of the airflow channel along the front and back horizontal direction. The multiple foam metal plates and the discharge electrode are respectively installed on the air electrostatic disinfection and purification module frame, and the air electrostatic disinfection and purification module frame is inserted into the air duct with a gap fit.

[0010] The present invention relates to a pathogen nucleic acid aerosol purification device, wherein the thickness of the foam metal plate is 5mm-15mm.

[0011] This invention relates to a pathogen nucleic acid aerosol purification device. A coarse filter at the air inlet at the bottom of the casing initially removes larger particles. The airflow then enters an electrostatic disinfection and purification module, where a high-voltage electric field kills and captures microorganisms. A microporous filter membrane made of H14 grade high-efficiency filter material further captures any remaining nucleic acid fragments from the killed microorganisms, ensuring the final purification effect. An ozone removal filter removes ozone from the airflow. The device also features multiple centrifugal fans arranged horizontally and alternately at intervals along the upper part of the duct. The air inlet of each centrifugal fan is located within the duct. Inside, the air outlet of each centrifugal fan is connected to the air inlet of an arc-shaped jet pipe. The arc-shaped jet pipe is set along the front-to-back direction, and its cross-section along the left-to-right vertical direction is rectangular. The air outlet end of each arc-shaped jet pipe is fixedly connected to the upper part of the front panel. Each arc-shaped jet pipe is a curved arc with a lower air inlet end and a higher air outlet end. Because the arc-shaped jet pipe at the air outlet is designed as an arc, and the front panel is an arc-shaped plate that gradually bends forward from bottom to top, this design can produce a better fluid adhesion effect and can drive the air around the front panel to flow with the air sprayed from the air outlet, enhance the air flow in the entire biological laboratory, and avoid eliminating dead corners. Therefore, the pathogen nucleic acid aerosol purification device of this utility model has the characteristics of enabling full circulation of air in the biological laboratory without dead corners, ensuring that the air circulation is as free of eddies and cross disturbances as possible, and efficiently disinfecting and removing nucleic acid fragments, viruses and bacteria in the aerosols in the biological laboratory air in a very short time, and efficiently removing suspended particulate matter in the biological laboratory air.

[0012] The pathogen nucleic acid aerosol purification device of this utility model will be further described in detail below with reference to the accompanying drawings. Attached Figure Description

[0013] Figure 1 This is a front view of the schematic diagram of the pathogen nucleic acid aerosol purification device of this utility model;

[0014] Figure 2 for Figure 1 Side view sectional view;

[0015] Figure 3 for Figure 1 Top view;

[0016] Figure 4 This is an enlarged front view of the foam metal plate part in the air electrostatic disinfection and purification module of the pathogen nucleic acid aerosol purification device of this utility model.

[0017] Figure 5 This is a front view of the pathogen nucleic acid aerosol purification device of this utility model after it is installed in a biological laboratory;

[0018] Figure 6 for Figure 5 Top view. Detailed Implementation

[0019] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 As shown, the pathogen nucleic acid aerosol purification device of this utility model includes a housing, which includes a frame. The frame is provided with a front panel 2, a left side panel 3, a right side panel 4, a rear baffle 5, and a top cover 6. The front panel 2 is an arc-shaped panel that gradually bends forward from bottom to top. The rear baffle 5 and the top cover 6 are rectangular. The surface of the rear baffle 5 is located in the left and right vertical direction, and the surface of the top cover 6 is located in the horizontal direction.

[0020] The bottom of the casing is an air inlet 8. The front panel 2, left side panel 3, right side panel 4, rear baffle 5 and top cover 6 form an air duct 7 that allows air to flow from bottom to top. Multiple centrifugal fans 10 are arranged side by side at intervals along the left and right horizontal direction in the upper part of the air duct 7. The air inlet of each centrifugal fan 10 is located in the air duct 7. The air outlet of each centrifugal fan 10 is connected to the air inlet of the arc-shaped jet pipe 15. The arc-shaped jet pipe 15 is arranged along the front and back direction. The cross section of the arc-shaped jet pipe 15 along the left and right vertical direction is rectangular. The air outlet end of each arc-shaped jet pipe 15 is fixedly connected to the upper part of the front panel 2. Each arc-shaped jet pipe 15 is a curved arc with a lower air inlet end and a higher air outlet end.

[0021] An ozone deodorization filter 11 is installed horizontally below the centrifugal fan 10 in the air duct 7. A microporous filter membrane 12 made of H14 grade high-efficiency filter material is installed horizontally below the ozone deodorization filter 11 in the air duct 7. An air electrostatic disinfection and purification module 13 is installed horizontally below the microporous filter membrane 12 in the air duct 7. A coarse filter 14 is installed at the air inlet 8 at the bottom of the housing.

[0022] In use, a pathogen nucleic acid aerosol purification device 1 can be suspended and fixed on the upper part of a wall inside the biological laboratory 20. The rear baffle 5 of the pathogen nucleic acid aerosol purification device 1 is attached to the corresponding wall. The air inlet of the pathogen nucleic acid aerosol purification device 1 is arranged facing the ground inside the biological laboratory 20. The top cover 6 of the pathogen nucleic acid aerosol purification device 1 is attached to the roof inside the biological laboratory 20 or located near the roof inside the biological laboratory 20.

[0023] Furthermore, a pathogen nucleic acid aerosol purification device 1 can be installed on the lower part of another wall opposite to the wall where a pathogen nucleic acid aerosol purification device 1 is fixed in the biological laboratory 20. The rear baffle 5 of the pathogen nucleic acid aerosol purification device 1 is attached to the corresponding wall. The air inlet of the pathogen nucleic acid aerosol purification device 1 is arranged facing the roof inside the biological laboratory 20. The top cover 6 of the pathogen nucleic acid aerosol purification device 1 is attached to or located near the ground inside the biological laboratory 20.

[0024] As a further improvement of this utility model, the bottom or side of the front panel 2 is connected to the frame by a hinge, the upper part of the front panel 2 is connected to the frame, the left side panel 3 or the right side panel 4 by screws or door latches, and an airtight strip is provided at the edge of the front panel 2.

[0025] As a further improvement of this utility model, a pathogen nucleic acid aerosol purification device 1 is suspended and fixed on the upper part of the west side of the north wall inside the above-mentioned biological laboratory 20, and a pathogen nucleic acid aerosol purification device 1 is fixed on the lower part of the east side of the south wall inside the biological laboratory 20.

[0026] As a further improvement of this utility model, the number of centrifugal fans 10 is 3-5, the centrifugal fans 10 are adjustable air volume fans, the height of the casing along the vertical direction is 1500mm-1700mm, the width of the casing along the horizontal direction is 1400mm-1900mm, the width of the top of the casing along the front-back direction is 600mm-800mm, and the width of the bottom of the casing along the front-back direction is 300mm-500mm.

[0027] As a further improvement of this utility model, the height from the ground to the roof in the above-mentioned biological laboratory 20 is 2500mm-2700mm, and the ground area in the biological laboratory 20 is 30-50 square meters.

[0028] As a further improvement of this utility model, the above-mentioned air electrostatic disinfection and purification module 13 includes multiple foam metal plates 16 whose surfaces can be traversed by airflow. The surfaces of the foam metal plates 16 are arranged along the front-to-back vertical direction, and airflow channels 17 are provided between the foam metal plates 16. A discharge electrode 18 is provided in the middle of the airflow channel 17 along the front-to-back horizontal direction. The multiple foam metal plates 16 and the discharge electrode 18 are respectively installed on the air electrostatic disinfection and purification module frame, and the air electrostatic disinfection and purification module frame is inserted into the air duct 7 with a gap fit.

[0029] Compared to the ordinary anode plates used in traditional electrostatic precipitators, where the electric field is only distributed on the surface, the porous structure of the foam metal plate 16 is three-dimensionally distributed, thus creating a three-dimensional electric field. Furthermore, its spike-like shape alters the spatial electric field, transforming it into a field similar to that formed by sharp points, increasing the local electric field strength and enhancing the breaking effect of nucleic acid bonds. Simultaneously, compared to ordinary anode plates, the foam metal plate 16 has a much larger dust collection area for the same volume, equivalent to 50 times that of ordinary anode plates, significantly improving the efficiency of capturing nucleic acid fragments, viruses, and bacterial microorganisms present in the air of the biological laboratory 20.

[0030] As a further improvement of this utility model, the thickness of the foam metal plate 16 is 5mm-15mm.

[0031] According to the relevant design specifications for ventilation in safety laboratories, the air circulation volume for disinfection and purification in Biological Laboratory 20 must meet the requirement of at least 15 disinfection and purification cycles per hour. If the preset conditions for Biological Laboratory 20 are: a usable area of ​​40 square meters, a room height of 2.6 meters, and an indoor air volume of 104 cubic meters, then the minimum total air circulation volume for disinfection and purification in Biological Laboratory 20 is: 104 * 15 = 1560 m³. 3 / h.

[0032] In the design process of this pathogen nucleic acid aerosol purification device, after multiple comprehensive comparisons of various unit placement methods and different equipment outlet designs, it was found that the best air circulation effect was achieved when two units were placed opposite each other in Room 20 of the biological laboratory, with the air inlet and outlet of the two units designed in opposite directions and symmetrically. This can minimize the problems of severe imbalance in circulation and numerous dead zones in Room 20 of the biological laboratory caused by traditional top-supply and top-return air fields and floor-standing air sterilizers.

[0033] The fan of the two pathogen nucleic acid aerosol purification device 1 can be designed to be 1000m. 3 / h and 600m 3 Two airflow rates are available: 1000 m³ / h. In a biological laboratory where no one is present and disinfection is required, the 1000 m³ / h rate can be used. 3 The 600 m³ / h airflow mode maximizes the number of indoor air circulations; when people and machines coexist, the 600 m³ / h airflow mode is used to keep the air in the biological laboratory 20 in a relatively stable state while disinfecting and purifying. The air purification circulation times for the 40 square meter, 2.6-meter-high biological laboratory 20 room are 19 and 12 respectively under the two airflow modes.

[0034] The air electrostatic disinfection and purification module 13 and coarse filter 14 of the pathogen nucleic acid aerosol purification device 1 can be manually cleaned, and the microporous filter membrane 12 made of H14 grade high-efficiency filter material can be replaced periodically according to the usage time.

[0035] In summary, the diagonal arrangement of the inlet and outlet of the two pathogen nucleic acid aerosol purification devices 1 ensures that the airflow effectively fills the entire biological laboratory 20 without forming eddies. This guarantees that all airflow within the biological laboratory 20 can smoothly enter the inlet of the pathogen nucleic acid aerosol purification device 1, and that the airflow of a single pathogen nucleic acid aerosol purification device 1 is 1000 m³ / s. 3 Under operating conditions of / h, more than 97% of the airflow velocity in the biological laboratory is greater than 0.05m / s.

[0036] The microbial inactivation principle of the air electrostatic disinfection and purification module 13 of this utility model is as follows.

[0037] The air electrostatic disinfection and purification module 13 of this utility model inactivates microorganisms mainly through the following two aspects:

[0038] (1) The air electrostatic disinfection and purification module 13 can continuously release plasma. The inactivating components (active groups, charged particles and electric fields, etc.) in the plasma cause microorganisms to die rapidly through biological damage (cell membrane damage, biological macromolecule damage and microbial sublethality).

[0039] (2) The air electrostatic disinfection and purification module 13 can continuously release electric field force through a strong electric field of 30,000 volts to produce a polarization effect on microorganisms and nucleic acids. The active tissues of microorganisms are instantly broken by the strong electric field, causing them to be inactivated. At the same time, it affects the nucleic acid sequence order, further preventing the possibility of microbial amplification and replication.

[0040] (3) Microbial killing via plasma: The air electrostatic disinfection and purification module 13 can release a large amount of plasma through a high-voltage electric field. Plasma is a conductive fluid that is electrically neutral and composed of electrons, ions, free radicals, excited-state particles, etc. Its composition is complex and diverse, and its properties are active. In addition to high-energy particles, ultraviolet rays and high-energy electric fields, there are many active particles that are excited or unexcited. When these active particles come into contact with microorganisms, a series of complex biochemical reactions and physical processes will occur between them and the biofilm.

[0041] From a macroscopic perspective, the sterilization process of atmospheric pressure low-temperature plasma is actually the interaction between the active components generated by the plasma (such as positive and negative ions, active groups, charged particles and electric fields, ultraviolet light, etc.) and biological macromolecules (such as proteins, DNA, lipids, etc.), causing changes in cellular components (such as cell walls, cell membranes, cytoplasm, etc.), and ultimately leading to cell death. The active components of the plasma either inactivate and denature biological macromolecules in a short period of time, or alter the level of oxidative stress within the cell, thereby achieving the purpose of microbial inactivation.

[0042] The main microbial inactivation components of plasma

[0043] In the sterilization process of atmospheric pressure low-temperature plasma, active groups, charged particles, and electric fields are the main factors of concern.

[0044] 1) Active groups

[0045] The current mainstream theory on the sterilization mechanism of atmospheric pressure low-temperature plasma believes that reactive oxygen species (ROS) and reactive nitrogen species (RNS) play the main roles in inducing cell death. Table 1 shows the common ROS and RNS in plasma.

[0046] Table 1 Common ROS / RNS in Plasma

[0047]

[0048]

[0049] 2) Charged particles and electric fields

[0050] During plasma generation, an electric field exists between two high-voltage electrodes, and the working gas dissociates between the electrodes, forming numerous charged particles. Related studies have shown that when bacterial samples are placed in a dielectric barrier discharge gap, the plasma transfers charged matter to the bacterial surface, while the high electric field intensity also has a certain impact on the bacteria. Plasma can disrupt the external structure of microorganisms, causing leakage of proteins, nucleic acids, and K+ from the cytoplasm, with charged particles and active substances playing a major role.

[0051] Biological damage of plasma to microorganisms: When plasma acts on microorganisms, different tissues of the microorganisms will produce different biological responses. The biological response mechanism of cells can be summarized into the following three points:

[0052] 1) Cell membrane damage theory

[0053] When the active substances generated by plasma act on the cell membrane, lipids on the cell membrane undergo peroxidation, and the cell membrane potential changes. This increases cell membrane permeability, causing more plasma active components from the outside to enter the cell, resulting in leakage of cell contents. At this point, the cell membrane collapses or becomes defective, disrupting normal physiological functions and causing the cell membrane to rupture, leading to apoptosis or even cell death.

[0054] 2) Biomolecular damage theory

[0055] When the active components of the plasma act on cells, they cause oxidative damage to biological macromolecules such as proteins, nucleic acids, and lipids, leading to complex biochemical reactions such as protein denaturation, DNA oxidative degradation, and lipid peroxidation, which in turn have a significant impact on the physiological functions of biological tissues.

[0056] 3) Microbial sublethal theory

[0057] Microorganisms treated with plasma did not show obvious morphological damage, but their metabolic capacity was weakened, their growth was slowed or stopped, and the activity of metabolic enzymes was destroyed, ultimately leading to microbial death. Among these factors, plasma-induced cellular lipid peroxidation may be the main factor causing the cells to be in a sublethal state.

[0058] This invention relates to a pathogen nucleic acid aerosol purification device. A coarse filter 14 at the air inlet 8 at the bottom of the casing initially removes larger particles of coarse matter. The airflow then enters the electrostatic disinfection and purification module 13, where a high-voltage electric field kills and captures microorganisms. A microporous filter membrane 12 made of H14 grade high-efficiency filter material further captures any remaining nucleic acid fragments from the disinfected microorganisms, ensuring the final purification effect. An ozone-removing filter 11 removes ozone from the airflow. The device also features multiple centrifugal fans 10 arranged horizontally and spaced apart in the upper part of the air duct 7. The air inlet of each centrifugal fan 10 is located within the air duct 7. Each centrifugal fan 10 has its outlet connected to the inlet of an arc-shaped jet pipe 15. The arc-shaped jet pipe 15 is arranged along the front-to-back direction and has a rectangular cross-section along the left-to-right vertical direction. The outlet end of each arc-shaped jet pipe 15 is fixedly connected to the upper part of the front panel 2. Each arc-shaped jet pipe 15 is a curved shape with a lower inlet end and a higher outlet end. Because the arc-shaped jet pipe 15 at the outlet is designed as an arc, and the front panel 2 is an arc-shaped plate that gradually bends forward from bottom to top, this design can generate a better fluid adhesion effect and can drive the air around the front panel 2 to flow with the air ejected from the outlet, thereby strengthening the airflow in the entire biological laboratory 20 and avoiding the elimination of dead corners. Therefore, the pathogen nucleic acid aerosol purification device of this utility model has the characteristics of allowing the air in the biological laboratory to circulate fully without dead corners, and allowing the air to circulate as much as possible without eddies or cross disturbances. It can efficiently disinfect and remove nucleic acid fragments, viruses and bacteria in the aerosols in the air of the biological laboratory in a very short time, and efficiently remove suspended particulate matter in the air of the biological laboratory.

Claims

1. A pathogen nucleic acid aerosol purification device, characterized in that: Includes a housing, which includes a frame. The frame is provided with a front panel (2), a left side panel (3), a right side panel (4), a rear baffle (5), and a top cover (6). The front panel (2) is an arc-shaped panel that gradually bends forward from bottom to top. The rear baffle (5) and the top cover (6) are rectangular. The surface of the rear baffle (5) is located in the left and right vertical direction, and the surface of the top cover (6) is located in the horizontal direction. The bottom of the casing is an air inlet (8). The front panel (2), left side panel (3), right side panel (4), rear baffle (5) and top cover (6) form an air duct (7) that allows air to flow from bottom to top. Multiple centrifugal fans (10) are arranged side by side in the upper part of the air duct (7) along the horizontal direction. The air inlet of each centrifugal fan (10) is located in the air duct (7). The air outlet of each centrifugal fan (10) is connected to the air inlet of the arc-shaped jet pipe (15). The arc-shaped jet pipe (15) is arranged along the front and back direction. The cross section of the arc-shaped jet pipe (15) along the vertical direction is rectangular. The air outlet end of each arc-shaped jet pipe (15) is fixedly connected to the upper part of the front panel (2). Each arc-shaped jet pipe (15) is a curved arc with a low air inlet end and a high air outlet end. An ozone deodorization filter (11) is installed horizontally below the centrifugal fan (10) in the air duct (7). A microporous filter membrane (12) made of H14 grade high-efficiency filter material is installed horizontally below the ozone deodorization filter (11) in the air duct (7). An air electrostatic disinfection and purification module (13) is installed horizontally below the microporous filter membrane (12) in the air duct (7). A coarse filter (14) is installed at the air inlet (8) at the bottom of the housing.

2. The pathogen nucleic acid aerosol purification device according to claim 1, characterized in that: The bottom or side of the front panel (2) is connected to the frame by hinges, and the upper part of the front panel (2) is connected to the frame, the left side panel (3) or the right side panel (4) by screws or door latches. An airtight strip is provided at the edge of the front panel (2).

3. The pathogen nucleic acid aerosol purification device according to claim 2, characterized in that: The number of centrifugal fans (10) is 3-5. The centrifugal fans (10) are adjustable air volume fans. The height of the casing along the vertical direction is 1500mm-1700mm, the width of the casing along the horizontal direction is 1400mm-1900mm, the width of the top of the casing along the front-back direction is 600mm-800mm, and the width of the bottom of the casing along the front-back direction is 300mm-500mm.

4. The pathogen nucleic acid aerosol purification device according to claim 1, 2, or 3, characterized in that: The air electrostatic disinfection and purification module (13) includes multiple foam metal plates (16) with airflow passing through their surfaces. The surfaces of the foam metal plates (16) are arranged vertically along the front and back. An airflow channel (17) is provided between the foam metal plates (16). A discharge electrode (18) is provided in the middle of the airflow channel (17) along the front and back horizontal direction. The multiple foam metal plates (16) and discharge electrode (18) are respectively installed on the air electrostatic disinfection and purification module frame. The air electrostatic disinfection and purification module frame is inserted into the air duct (7) with a gap fit.

5. The pathogen nucleic acid aerosol purification device according to claim 4, characterized in that: The thickness of the foam metal plate (16) is 5mm-15mm.

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