Cell culture dish convenient for separating extracellular matrix

Abstract

The utility model discloses a cell culture dish convenient to separate extracellular matrix, and relates to the technical field of cell culture dishes, the cell culture dish comprises a sterile dish body, support blocks are symmetrically fixed on the inner side of the sterile dish body, first guide pillars are fixed at the upper ends of the two support blocks, a filter frame is slidably connected between the two first guide pillars and located on the inner side of the sterile dish body, plugging strips are fixed to the outer sides of the two supporting blocks correspondingly, a supporting cylinder is fixed between the two plugging strips, a through groove is formed in the center of the interior of the filter frame, filter membranes are symmetrically fixed to the positions, located on the outer side of the through groove, of the inner side of the filter frame, and a supporting shaft is rotationally connected to the center of the bottom end of the sterile vessel body. The upper end of the supporting shaft penetrates through the supporting cylinder, extends to the inner side of the filtering frame and is symmetrically fixed with first stirring plates. According to the cell culture dish convenient for separating the extracellular matrix, provided by the utility model, particulate matters in a culture solution are filtered and removed through the filter membrane, the cleanliness of the culture solution is ensured, and the filtered culture solution is further mixed conveniently by utilizing the second stirring plate.

Description

Technical Field

[0001] This invention relates to the field of cell culture dish technology, and in particular to a cell culture dish that facilitates the separation of extracellular matrix. Background Technology

[0002] Biological gene research refers to the scientific field that conducts in-depth research on the structure, function, and regulatory mechanisms of genes within organisms. Developments in this field are of great significance for understanding the fundamental principles of life, the mechanisms of disease, and the genetic diversity of organisms. Biological gene research typically requires cell culture, which is conducted using cell culture dishes. During cell culture, the extracellular matrix plays a crucial role in cell growth, differentiation, and function.

[0003] For example, Chinese utility model patent (CN205329067U) discloses a cell culture dish, which describes: "It includes a cover plate and a main body. The cover plate is hinged to the main body through a button shaft. A push switch is installed on the button shaft. The cover plate and the main body are interlocked and locked together by a protrusion on the cover plate and a groove on the main body. By setting a push switch with a button shaft, not only is the operation simple, but the culture dish is also protected from contamination to the greatest extent."

[0004] However, the above-mentioned culture dishes are not easy to filter out particulate matter in the culture medium during use, which cannot guarantee the cleanliness of the culture medium and is not conducive to normal cell growth. This makes it inconvenient and inefficient to use the suction filtration technique to separate the cells from the extracellular matrix in the culture dish. Therefore, this application proposes a cell culture dish that facilitates the separation of the extracellular matrix. Utility Model Content

[0005] Therefore, it is necessary to provide a cell culture dish that facilitates the separation of the extracellular matrix to address the aforementioned technical problems. Through the overall structural design, the culture medium can be easily introduced into the sterile dish body after the sealed cap is opened. Then, the filter membrane can be used to filter and remove particulate matter in the culture medium, ensuring the cleanliness of the culture medium. Furthermore, the second stirring plate can be used to further mix the filtered culture medium, ensuring the uniform distribution of nutrients, gases, and pH adjusters in the culture medium. This promotes uniform cell growth throughout the culture process and facilitates efficient separation of cells from the extracellular matrix when using vacuum filtration techniques.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] A cell culture dish that facilitates the separation of extracellular matrix, which is used for cell culture and extracellular matrix separation;

[0008] The device includes a sterile dish body, a sealing cap at the top of the sterile dish body, symmetrical support blocks fixed inside the sterile dish body, a first guide post fixed at the top of each of the two support blocks, a filter frame slidably connected between the two first guide posts and inside the sterile dish body, the bottom of the filter frame fitting against the two support blocks, a sealing strip fixed at the outer side of each of the two support blocks, a support cylinder fixed between the two sealing strips, a through groove at the center of the filter frame, the sealing strip and the support cylinder being located inside the through groove and slidably connected to the through groove, a filter membrane symmetrically fixed inside the filter frame and outside the through groove, and a support shaft rotatably connected at the center of the bottom of the sterile dish body, the upper end of the support shaft extending through the support cylinder to the inside of the filter frame and symmetrically fixed with a first stirring plate.

[0009] As a preferred embodiment of the cell culture dish for easy separation of extracellular matrix provided by this utility model, a geared motor is fixed inside the lower end of the sterile dish body, and the output end of the geared motor is aligned with the axis of the support shaft and fixed to the support shaft.

[0010] As a preferred embodiment of the cell culture dish for easy separation of extracellular matrix provided by this utility model, a scraper is fixed to the lower end of each of the two first stirring plates, and the lower end of each scraper is attached to the upper end of the filter membrane and the sealing strip.

[0011] As a preferred embodiment of the cell culture dish for easy separation of extracellular matrix provided by this utility model, a second stirring plate is symmetrically fixed on the outer side of the support and at the lower end of the filter frame, and a soft handle is symmetrically fixed on the upper end of the inner side of the filter frame.

[0012] As a preferred embodiment of the cell culture dish for easy separation of extracellular matrix provided by this utility model, the sterile dish body is provided with symmetrical guide grooves on the outer side, and the lower end of the sealing cap is symmetrically fixed with L-shaped support plates.

[0013] As a preferred embodiment of the cell culture dish for easy separation of extracellular matrix provided by this utility model, a second guide post is provided on the inner side of each of the two guide grooves. The second guide post is fixed to the sterile dish body. The lower ends of the two L-shaped support plates are respectively located on the inner side of the two guide grooves and are slidably connected to the second guide post. A first spring is sleeved on the outer side of each second guide post. The upper end of the first spring is fixed to the L-shaped support plate, and the lower end of the first spring is fixed to the sterile dish body. A pull rod is slidably connected to the inner side of the lower end of each of the two L-shaped support plates. One end of the pull rod extends into the interior of the sterile dish body and is slidably connected to the sterile dish body. A second spring is fixed between the outer side of the other end of the pull rod and the L-shaped support plate.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The cell culture dish provided by this invention facilitates the separation of the extracellular matrix. Through its overall structural design, the culture medium can be easily introduced into the sterile dish body after the sealing cap is opened. The filter membrane then removes particulate matter from the culture medium, ensuring its cleanliness. Furthermore, the second stirring plate facilitates further mixing of the filtered culture medium, ensuring uniform distribution of nutrients, gases, and pH adjusters. This promotes uniform cell growth throughout the culture process and allows for efficient separation of cells from the extracellular matrix using subsequent vacuum filtration techniques. Attached Figure Description

[0016] To more clearly illustrate the solutions in this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 A schematic diagram of the overall structure of the cell culture dish that facilitates the separation of extracellular matrix provided by this utility model;

[0018] Figure 2 A schematic diagram of the structure of the cell culture dish with the sealing cap moved upwards to facilitate the separation of the extracellular matrix, as provided by this utility model;

[0019] Figure 3 A schematic diagram of the structure of the cell culture dish filter frame for easy separation of extracellular matrix provided by this utility model before installation;

[0020] Figure 4 A schematic diagram of the structure of the cell culture dish filter frame for easy separation of extracellular matrix provided by this utility model after installation;

[0021] Figure 5 A schematic diagram of the structure between the L-shaped support plate and the guide groove of the cell culture dish for facilitating the separation of extracellular matrix provided by this utility model.

[0022] The markings in the diagram are explained as follows:

[0023] 1. Sterile dish body; 2. Sealing cap; 3. Support block; 4. First guide post; 5. Sealing strip; 6. Support cylinder; 7. Filter frame; 8. Through groove; 9. Filter membrane; 10. Soft handle strip; 11. Gear motor; 12. Support shaft; 13. First stirring plate; 14. Scraper; 15. Second stirring plate; 16. L-shaped support plate; 17. Guide groove; 18. Second guide post; 19. First spring; 20. Pull rod; 21. Second spring. Detailed Implementation

[0024] As described in the background art, when using the above-mentioned culture dishes, it is not easy to filter out particulate matter in the culture medium, which cannot guarantee the cleanliness of the culture medium and is not conducive to normal cell growth. This results in inconvenient operation and low efficiency when using vacuum filtration technology to separate the cells in the culture dish from the extracellular matrix.

[0025] To solve this technical problem, this utility model provides a cell culture dish that facilitates the separation of extracellular matrix, which is used for cell culture and extracellular matrix separation;

[0026] The device includes a sterile dish body 1, with a sealing cap 2 at the upper end. Symmetrically fixed support blocks 3 are attached to the inner side of the sterile dish body 1. A first guide post 4 is fixed to the upper end of each of the two support blocks 3. A filter frame 7 is slidably connected between the two first guide posts 4 and inside the sterile dish body 1. The bottom end of the filter frame 7 is in contact with the two support blocks 3. A sealing strip 5 is fixed to the outer side of each of the two support blocks 3. A support cylinder 6 is fixed between the two sealing strips 5. A through groove 8 is opened at the center of the filter frame 7. The sealing strips 5 and the support cylinder 6 are both located inside the through groove 8 and slidably connected to it. A filter membrane 9 is symmetrically fixed to the inner side of the filter frame 7 and outside the through groove 8. A support shaft 12 is rotatably connected to the center of the bottom end of the sterile dish body 1. The upper end of the support shaft 12 extends through the support cylinder 6 to the inner side of the filter frame 7 and is symmetrically fixed with a first stirring plate 13.

[0027] The cell culture dish provided by this invention facilitates the separation of the extracellular matrix. Through its overall structural design, the culture medium can be easily introduced into the inner side of the sterile dish body 1 after the sealing cap 2 is opened. Then, the filter membrane 9 is used to filter and remove particulate matter in the culture medium, ensuring the cleanliness of the culture medium. It also facilitates further mixing of the filtered culture medium, ensuring the uniform distribution of nutrients, gases and pH adjusters in the culture medium, thereby promoting uniform cell growth throughout the culture process. This makes it easier to efficiently separate the cells from the extracellular matrix in the culture dish when using vacuum filtration technology.

[0028] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0029] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0030] Example 1:

[0031] Please refer to Figure 1-5A cell culture dish for easy separation of extracellular matrix includes a sterile dish body 1, a sealing cap 2 snapped onto the upper end of the sterile dish body 1, support blocks 3 symmetrically fixed inside the sterile dish body 1, a first guide post 4 fixed to the upper end of each of the two support blocks 3, a filter frame 7 slidably connected between the two first guide posts 4 and inside the sterile dish body 1, the bottom end of the filter frame 7 being attached to the two support blocks 3, the support blocks 3 facilitating the support of the filter frame 7, and in order to filter the culture medium introduced into the sterile dish body 1, a through groove 8 is opened in the center of the filter frame 7, and a filter membrane 9 is symmetrically fixed inside the filter frame 7 and outside the through groove 8.

[0032] To facilitate the sealing of the through groove 8 and ensure that the culture medium flows downward after being filtered through the filter membrane 9, sealing strips 5 are fixed to the outer sides of the two support blocks 3, and a support cylinder 6 is fixed between the two sealing strips 5. The sealing strips 5 and the support cylinder 6 are located inside the through groove 8 and are slidably connected to the through groove 8. In order to facilitate the gripping of the filter frame 7, soft handle strips 10 are symmetrically fixed to the upper end of the inner side of the filter frame 7, so as to facilitate the installation and disassembly of the filter frame 7.

[0033] To facilitate stirring inside the filter frame 7 and thus accelerate filtration, a support shaft 12 is rotatably connected to the center of the bottom of the sterile dish body 1. The upper end of the support shaft 12 extends through the support cylinder 6 to the inside of the filter frame 7 and is symmetrically fixed with first stirring plates 13. To further reduce clogging caused by the accumulation of particles on the upper end of the filter membrane 9, scrapers 14 are fixed to the lower ends of the two first stirring plates 13. The lower ends of the two scrapers 14 are in contact with the upper ends of the filter membrane 9 and the sealing strip 5. It can be seen that the upper ends of the filter membrane 9 and the sealing strip 5 are at the same horizontal line. Thus, the scrapers 14 can scrape off the particles accumulated on the upper end of the filter membrane 9 while avoiding the sealing strip 5 from obstructing the scrapers 14.

[0034] To facilitate the automatic rotation of the support shaft 12, a geared motor 11 is fixed inside the lower end of the sterile dish body 1. The output end of the geared motor 11 is aligned with the axis of the support shaft 12 and fixed to the support shaft 12. When the culture medium accumulates at the lower end of the inner side of the sterile dish body 1 after being filtered by the filter membrane 9, in order to further mix it and ensure that the nutrients, gases and pH adjusters in the culture medium are evenly distributed, thereby promoting uniform cell growth throughout the culture process, it is convenient to efficiently separate the cells in the culture dish from the extracellular matrix when using the suction filtration technique later. A second stirring plate 15 is symmetrically fixed on the outside of the support shaft 12 and at the lower end of the filter frame 7.

[0035] Example 2:

[0036] The cell culture dish provided in Example 1 for facilitating the separation of the extracellular matrix has been further optimized, specifically, as follows: Figure 1 , 2As shown in Figure 5, when the sterile dish body 1 is sealed by the sealing cap 2, the sealing cap 2 and the sterile dish body 1 are connected by a snap-fit ​​and a slot. When the sealing cap 2 is opened, in order to prevent the sealing cap 2 from separating from the sterile dish body 1, guide grooves 17 are symmetrically opened on the outer side of the sterile dish body 1. L-shaped support plates 16 are symmetrically fixed at the lower end of the sealing cap 2. Second guide posts 18 are provided on the inner side of both guide grooves 17. The second guide posts 18 are fixed to the sterile dish body 1. The lower ends of the two L-shaped support plates 16 are located on the inner side of the two guide grooves 17 and are slidably connected to the second guide posts 18.

[0037] After the sealing cap 2 is released from the snap connection with the sterile dish body 1 and the sealing cap 2 is moved upward, in order to facilitate the quick reset of the sealing cap 2 when the sealing cap 2 is released, a first spring 19 is sleeved on the outside of each second guide post 18. The upper end of the first spring 19 is fixed to the L-shaped support plate 16, and the lower end of the first spring 19 is fixed to the sterile dish body 1. When the sealing cap 2 is moved upward, in order to limit and fix the position of the sealing cap 2, a pull rod 20 is slidably connected inside the lower end of the two L-shaped support plates 16. One end of the pull rod 20 extends into the interior of the sterile dish body 1 and is slidably connected to the sterile dish body 1. A second spring 21 is fixed between the outer side of the other end of the pull rod 20 and the L-shaped support plate 16.

[0038] All of the above electrical components are electrically connected to the main controller and the power supply. The main controller can be a conventional known device such as a computer that performs control, and the existing publicly available power connection technology will not be described in detail here.

[0039] The process of using the cell culture dish for easy separation of extracellular matrix provided by this utility model is as follows: First, pull up the sealing cap 2 to release the connection between the sealing cap 2 and the sterile dish body 1. Then, move the sealing cap 2 up and stretch the first spring 19. During the upward movement of the sealing cap 2, pull out the two pull rods 20. After the sealing cap 2 has moved up, release the two pull rods 20 and use the elasticity of the second spring 21 to drive the pull rods 20 back to slide into the sterile dish body 1, thereby limiting and fixing the position of the sealing cap 2. When it is necessary to close the sealing cap 2, pull out the pull rods 20 and use the elasticity of the first spring 19 to drive the sealing cap 2 to quickly return to its original position. Press the sealing cap 2 again so that the sealing cap 2 can reconnect with the sterile dish body 1, thus completing the sealing of the sterile dish body 1.

[0040] After the sealing cover 2 is opened, the soft handle 10 is used to facilitate the installation or removal of the filter frame 7. The two first guide posts 4 are used to position the filter frame 7 during installation, and the two support blocks 3 provide support for the filter frame 7. After the filter frame 7 is installed, the sealing strip 5 and the support cylinder 6 are located inside the through groove 8 to seal it. Throughout the installation or removal of the filter frame 7, the positions of the first stirring plate 13, the scraper 14, and the second stirring plate 15 are as follows: Figure 3 As shown, avoid blocking filter frame 7;

[0041] After the filter frame 7 is installed, the geared motor 11 is started to drive the support shaft 12 to rotate, thereby controlling the movement of the first stirring plate 13, the scraper 14 and the second stirring plate 15. At this time, the culture medium is introduced into the sterile dish 1. The filter membrane 9 facilitates the filtration of the culture medium, and the first stirring plate 13 accelerates the filtration. The scraper 14 facilitates the removal of particulate matter buildup on the upper end of the filter membrane 9 to reduce clogging. The second stirring plate 15 facilitates further mixing of the filtered culture medium, ensuring that nutrients, gases and pH adjusters in the culture medium are evenly distributed, thereby promoting uniform cell growth throughout the culture process. This makes it easier to efficiently separate the cells from the extracellular matrix in the culture dish when using vacuum filtration technology later.

Claims

1. A cell culture dish for easy separation of extracellular matrix, characterized in that, The system includes a sterile dish body (1), with a sealing cap (2) at the upper end of the sterile dish body (1). Symmetrical support blocks (3) are fixed to the inner side of the sterile dish body (1). A first guide post (4) is fixed to the upper end of each of the two support blocks (3). A filter frame (7) is slidably connected between the two first guide posts (4) and inside the sterile dish body (1). The bottom end of the filter frame (7) is attached to the two support blocks (3). A sealing strip (5) is fixed to the outer side of each of the two support blocks (3). A support cylinder (6) is fixed between the filter frame (7). A through groove (8) is opened at the center inside the filter frame (7). The sealing strip (5) and the support cylinder (6) are located inside the through groove (8) and are slidably connected to the through groove (8). A filter membrane (9) is symmetrically fixed inside the filter frame (7) and outside the through groove (8). A support shaft (12) is rotatably connected at the center of the bottom end of the sterile dish body (1). The upper end of the support shaft (12) extends through the support cylinder (6) to the inside of the filter frame (7) and is symmetrically fixed with a first stirring plate (13).

2. The cell culture dish for facilitating the separation of extracellular matrix according to claim 1, characterized in that, A geared motor (11) is fixed inside the lower end of the sterile dish body (1). The output end of the geared motor (11) is aligned with the axis of the support shaft (12) and fixed to the support shaft (12).

3. The cell culture dish for facilitating the separation of extracellular matrix according to claim 1, characterized in that, The lower ends of the two first stirring plates (13) are fixed with scrapers (14), and the lower ends of the two scrapers (14) are attached to the upper ends of the filter membrane (9) and the sealing strip (5).

4. The cell culture dish for facilitating the separation of extracellular matrix according to claim 1, characterized in that, A second stirring plate (15) is symmetrically fixed on the outside of the support shaft (12) and at the lower end of the filter frame (7), and a soft handle strip (10) is symmetrically fixed on the upper end of the inner side of the filter frame (7).

5. The cell culture dish for facilitating the separation of extracellular matrix according to claim 1, characterized in that, The sterile dish body (1) has symmetrically provided guide grooves (17) on its outer side, and the lower end of the sealing cap (2) is symmetrically fixed with L-shaped support plates (16).

6. The cell culture dish for facilitating the separation of extracellular matrix according to claim 5, characterized in that, The inner sides of the two guide grooves (17) are provided with second guide posts (18), which are fixed to the sterile dish body (1). The lower ends of the two L-shaped support plates (16) are located inside the two guide grooves (17) and are slidably connected to the second guide posts (18). The outer side of each second guide post (18) is provided with a first spring (19). The upper end of the first spring (19) is fixed to the L-shaped support plate (16), and the lower end of the first spring (19) is fixed to the sterile dish body (1). The inner sides of the lower ends of the two L-shaped support plates (16) are slidably connected with pull rods (20). One end of the pull rod (20) extends into the sterile dish body (1) and is slidably connected to the sterile dish body (1). The outer side of the other end of the pull rod (20) is fixed with a second spring (21) between it and the L-shaped support plate (16).

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