Glue making device for monoclonal antibody quality inspection

Abstract

The utility model discloses a glue making device for monoclonal antibody quality inspection, which comprises a bottom plate, two sides of the top of the bottom plate are fixedly connected with clamping seats, the opposite sides of the two clamping seats are provided with mounting grooves, glue making molds are arranged in the mounting grooves, the surfaces of the clamping seats are fixedly connected with a connecting shell, and the connecting shell is provided with a glue making cavity. The middle of the connecting shell is rotationally connected with a screw rod, the surface of the screw rod is in threaded connection with a connecting plate, the surface of the connecting plate is fixedly connected with a connecting column, one end of the connecting column penetrates through the connecting shell and one side of the clamping seat and extends into the mounting groove, and the end, extending into the mounting groove, of the connecting column is fixedly connected with a pressing plate; and one end of the screw extends out of the connecting shell and is fixedly connected with a rotating handle. The utility model relates to the technical field of biological detection, and discloses a glue making device for monoclonal antibody quality inspection, which avoids liquid leakage in a glue making process and improves the clamping efficiency at the same time.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, specifically a gel preparation device for monoclonal antibody quality testing. Background Technology

[0002] In the production and quality control of monoclonal antibodies (mAbs), gel electrophoresis is a crucial technology used for the separation, identification, and purification of mAbs. The first step in gel electrophoresis is gel preparation, currently primarily relying on manual operation. In this method, two glass plates are joined together as a mold, and then fixed to a gel preparation frame. However, insufficient sealing between the two glass plates during this process can lead to leakage. Furthermore, existing gel preparation frames typically require one hand to hold both plates and place them on the frame while the other hand maneuvers the frame to clamp one side of the plate, then the other side is secured. This inefficient clamping process, coupled with the possibility of relative displacement due to the inability to simultaneously fix both sides, compromises the seal. Therefore, a gel preparation device for monoclonal antibody quality control needs to be designed to improve both the sealing of the mold and its clamping efficiency. Utility Model Content

[0003] To address the aforementioned shortcomings, this invention provides a gel preparation device for monoclonal antibody quality inspection, which avoids leakage during the gel preparation process and improves clamping efficiency.

[0004] To achieve the above objectives, the present invention provides a gel preparation device for monoclonal antibody quality testing, comprising a base plate, clamps fixedly connected to the top two sides of the base plate, an installation groove provided on one side of the two clamps opposite each other, a gel preparation mold provided in the installation groove, a connecting shell fixedly connected to the surface of the clamps, a screw rotatably connected to the middle of the connecting shell, a connecting plate threadedly connected to the surface of the screw, a connecting post fixedly connected to the surface of the connecting plate, one end of the connecting post penetrating one side of the connecting shell and the clamps and extending into the installation groove, a pressure plate fixedly connected to the end of the connecting post extending into the installation groove, and a rotating handle fixedly connected to one end of the screw extending outside the connecting shell;

[0005] The glue-making mold includes a first glue-making plate and a second glue-making plate. The first glue-making plate has raised strips on its surface and at the edges on both sides and the bottom. The surface of the raised strips has sealing grooves. The second glue-making plate has a protrusion on its side close to the first glue-making plate and at the position corresponding to the sealing groove. A conductor is provided at the bottom of the raised strip at the bottom of the first glue-making plate, and one end of the conductor extends above the raised strip.

[0006] Preferably, a limiting seat is fixedly connected to the top center of the clamp, and a rubber comb is provided inside the limiting seat.

[0007] Preferably, a sponge pad is provided on the side of the pressure plate near the glue-making mold, and the cross-section of the connecting column is a rectangular structure.

[0008] Preferably, both ends of the connecting plate are slidably connected to the inner wall of the connecting housing.

[0009] Preferably, the connecting housing is located at the middle position in the height direction of the clamp.

[0010] Preferably, the bottom of the protrusion is provided with a receiving groove, and the conductor is disposed in the receiving groove.

[0011] Preferably, a sealing ring is provided on the surface of the sealing groove.

[0012] The present invention adopts the above-mentioned technical solution, and its beneficial effects include: This monoclonal antibody quality inspection gel-forming device, by setting a rotating handle in the middle of the gel-forming frame, allows for simultaneous clamping of both sides of the gel-forming mold by turning the rotating handle during clamping, ensuring uniform force on both sides and preventing relative movement between the two glass plates of the gel-forming mold, thus improving clamping efficiency; furthermore, by improving the structure of the gel-forming mold, protrusions are set on three sides of the first gel-forming plate, with sealing grooves on the surface of the protrusions. Simultaneously, a protrusion adapted to the sealing groove is set on one side of the second gel-forming plate, improving overall sealing performance and preventing relative movement between the first and second gel-forming plates, thus avoiding leakage during gel-forming. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a schematic diagram of the connection between the connecting plate and the connecting housing of this utility model. Figure 1 ;

[0015] Figure 3 This is a schematic diagram of the connection between the connecting plate and the connecting housing of this utility model. Figure 2 ;

[0016] Figure 4 This is a schematic diagram of the structure of the glue-making mold of this utility model.

[0017] In the diagram: 1-base plate, 2-clamping seat, 3-mounting groove, 4-glue forming mold, 41-first glue forming plate, 42-second glue forming plate, 43-protrusion, 44-sealing groove, 45-protrusion, 46-conductor, 47-accommodating groove, 5-connecting housing, 6-screw, 7-connecting plate, 8-connecting column, 9-pressure plate, 10-rotating handle, 11-limiting seat, 12-glue comb. Detailed Implementation

[0018] The present invention will be further described below with reference to the accompanying drawings.

[0019] Example 1: Please refer to Figure 1-4 This utility model provides a technical solution: a gel preparation device for monoclonal antibody quality inspection, including a base plate 1, with clamps 2 fixedly connected to the top two sides of the base plate 1, and an installation groove 3 provided on the opposite side of the two clamps 2. A gel preparation mold 4 is provided in the installation groove 3, and the width of the installation groove 3 must be greater than the sum of the thickness of the gel preparation mold 4 and the pressure plate 9 to ensure that the gel preparation mold 4 can be placed in the installation groove 3.

[0020] A connecting housing 5 is fixedly connected to the surface of the clamping base 2. A screw 6 is rotatably connected to the middle of the connecting housing 5. A connecting plate 7 is threadedly connected to the surface of the screw 6. A connecting post 8 is fixedly connected to the surface of the connecting plate 7. One end of the connecting post 8 passes through the connecting housing 5 and one side of the clamping base 2 and extends into the mounting groove 3. A pressure plate 9 is fixedly connected to the end of the connecting post 8 that extends into the mounting groove 3. One end of the screw 6 extends out of the connecting housing 5 and is fixedly connected to a rotating handle 10. By rotating the handle 10, the screw 6 is driven to rotate. The connecting plate 7 moves along the axial direction of the screw 6. When it moves toward the glue-making mold 4, the pressure plate 9 presses the two sides of the glue-making mold 4 together, which improves the clamping efficiency.

[0021] The glue-making mold 4 includes a first glue-making plate 41 and a second glue-making plate 42. The first glue-making plate 41 has raised strips 43 on its surface, particularly at its sides and bottom edges. Each raised strip 43 has a sealing groove 44 on its surface, forming a U-shape. The second glue-making plate 42 has a protrusion 45 on its side closest to the first glue-making plate 41, directly corresponding to the sealing groove 44. The raised strips 43 can be glued to the surface of the first glue-making plate 41, or they can be an integral structure. Similarly, the protrusion 45 can be glued to the surface of the second glue-making plate 42, or the protrusion 45 and the second glue-making plate 42 can be an integral structure, connected via the sealing groove 44. The protrusion 45 improves the sealing performance and ensures the alignment of the first gel-forming plate 41 and the second gel-forming plate 42, preventing relative movement during clamping. A conductor 46 is provided at the bottom of the protrusion 43 at the bottom of the first gel-forming plate 41, and a receiving groove 47 is provided at the bottom of the protrusion 43. The conductor 46 is placed in the receiving groove 47 to prevent the bottom of the gel-forming mold 4 from being unstable due to the protrusion of the bottom of the conductor 46. The conductor 46 is made of metal or conductive rubber, and one end of the conductor 46 extends above the protrusion 43 to ensure that the electric field can be evenly distributed during electrophoresis, thereby achieving molecular separation based on charge and size.

[0022] A limiting seat 11 is fixedly connected to the top center of the clamping seat 2. A glue comb 12 is provided inside the limiting seat 11 to ensure that the glue comb 12 is located in the middle position of the glue making mold 4.

[0023] A sponge pad is provided on the side of the pressure plate 9 near the glue-making mold 4 to cushion the surface and prevent indentations from forming on the surface of the glue-making mold 4. The cross-section of the connecting column 8 is rectangular to ensure that the connecting column 8 is always vertical.

[0024] The two ends of the connecting plate 7 are slidably connected to the inner wall of the connecting housing 5, which prevents the connecting plate 7 from rotating with the screw 6.

[0025] The connecting housing 5 is positioned at the middle of the height direction of the clamping seat 2 to ensure that the surface of the glue-making mold 4 is subjected to uniform pressure.

[0026] A sealing ring is provided on the surface of the sealing groove 44, which further improves the sealing performance.

[0027] In use, align the protrusion 45 on one side of the second glue-making plate 42 with the sealing groove 44 on the surface of the first glue-making plate 41 and put them together. Place the assembled glue-making mold 4 into the mounting groove 3 with one hand, and rotate the screw 6 by turning the handle 10 with the other hand. Press the two sides of the glue-making mold 4 with the pressure plate 9 to complete the clamping.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0029] The above description is merely a preferred embodiment of the prior art for fixing and installing this utility model, and is not intended to limit this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A gel preparation device for monoclonal antibody quality testing, comprising a base plate (1), characterized in that: The top two sides of the base plate (1) are fixedly connected to the clamps (2). The two clamps (2) are provided with an installation groove (3) on the opposite side. The installation groove (3) is provided with a glue-making mold (4). The surface of the clamps (2) is fixedly connected to the connecting housing (5). The middle part of the connecting housing (5) is rotatably connected to the screw (6). The surface of the screw (6) is threadedly connected to the connecting plate (7). The surface of the connecting plate (7) is fixedly connected to the connecting column (8). One end of the connecting column (8) passes through the connecting housing (5) and one side of the clamps (2) and extends into the installation groove (3). The end of the connecting column (8) extending into the installation groove (3) is fixedly connected to the pressure plate (9). One end of the screw (6) extends out of the connecting housing (5) and is fixedly connected to the rotating handle (10). The glue-making mold (4) includes a first glue-making plate (41) and a second glue-making plate (42). The first glue-making plate (41) has a raised strip (43) on its surface and at the edges on both sides and the bottom. The surface of the raised strip (43) has a sealing groove (44). The second glue-making plate (42) has a protrusion (45) on the side close to the first glue-making plate (41) and at the position corresponding to the sealing groove (44). A conductor (46) is provided at the bottom of the raised strip (43) at the bottom of the first glue-making plate (41). One end of the conductor (46) extends above the raised strip (43).

2. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: A limiting seat (11) is fixedly connected to the top center of the clamp (2), and a rubber comb (12) is provided inside the limiting seat (11).

3. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: The pressure plate (9) is provided with a sponge pad on the side near the glue-making mold (4), and the cross-section of the connecting column (8) is a rectangular structure.

4. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: The two ends of the connecting plate (7) are slidably connected to the inner wall of the connecting housing (5).

5. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: The connecting housing (5) is located at the middle position in the height direction of the clamp (2).

6. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: The bottom of the protrusion (43) is provided with a receiving groove (47), and the conductor (46) is disposed in the receiving groove (47).

7. The gel preparation device for monoclonal antibody quality testing according to claim 1, characterized in that: A sealing ring is provided on the surface of the sealing groove (44).

Images