Simple device for separating PBMC

Abstract

The utility model discloses a simple device for separating PBMC (peripheral blood mononuclear cells). The simple device comprises a pipe body and an extraction part arranged on the side wall of the pipe body, a through hole is formed in one side of the pipe body; one side of the through hole is communicated with the cavity in the pipe body, and the other side of the through hole is communicated with the outside; the extracting part comprises a first connecting part, a second connecting part and a third connecting part which are integrally connected, the first connecting part is arranged in the pipe body, the second connecting part is clamped to the hole wall of the through hole, and the third connecting part is arranged on the outer side of the pipe body. According to the utility model, the yield of the PBMC can be increased, and the number of other parenchyma cells such as red blood cells, granulocytes and platelets can be reduced, so that the purity of the PBMC is improved, and the operation is simpler and more convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of cell separation, and specifically relates to a simple device for separating PBMC. BACKGROUND

[0002] Peripheral blood mononuclear cells (PBMC) are cells with a single nucleus in peripheral blood, mainly including lymphocytes and monocytes. PBMC has been widely used in the fields of cell biology, immunology and transplantation therapy. At present, the commonly used methods for separating PBMC from peripheral blood include Ficoll density gradient centrifugation, Percoll density gradient centrifugation and HES sedimentation method, etc. Compared with other methods, the Ficoll separation method has low cost and simple operation, and is currently the main method for separating PBMC.

[0003] However, the Ficoll separation method currently used needs to be cautious and careful to suck the PBMC cell layer (white membrane layer), and if the operation is not proper, red blood cells, granulocytes and platelets are easily sucked, which affects the quality of the separated PBMC. INVENTION CONTENTS

[0004] The present application provides a simple device for separating PBMC. Since PBMC is separated from peripheral blood, and the peripheral blood sample is precious, the separation device in the present application can increase the yield of PBMC and reduce the number of other impurity cells such as red blood cells, granulocytes and platelets, so as to improve the purity of PBMC. In addition, the separation device in the present application has a side opening, which is more convenient to operate without the need to open the cover for suction.

[0005] The simple device for separating PBMC provided by the present application comprises a tube body and an extraction piece arranged on the side wall of the tube body; one side of the tube body is provided with a through hole; one side of the through hole is communicated with a cavity in the tube body, and the other side of the through hole is communicated with the outside world; the extraction piece comprises a first connecting part, a second connecting part and a third connecting part which are integrally connected; the first connecting part is arranged in the tube body, the second connecting part is clamped at the hole wall of the through hole, and the third connecting part is arranged outside the tube body; the extraction piece is pierced through the extraction piece and the cavity in the tube body from the outside world.

[0006] By adopting the above technical scheme, since the peripheral blood in the tube body is stratified during centrifugation, various stratifications such as PBMC cell layer, red blood cells, granulocytes and platelets are formed. In order to extract lymphocytes and monocytes in the PBMC cell layer, the extraction piece is pierced through the needle hole and communicated with the cavity in the tube body, so as to accurately suck and collect the PBMC cell layer in the tube body, increase the yield of PBMC, improve the purity of PBMC, and make the operation more simple and convenient.

[0007] Optionally, the third connecting part is a circular ring connected to the second connecting part, and the extraction member penetrates the first connecting part and the second connecting part from the outside to communicate with the cavity in the tube body.

[0008] By adopting the above technical solution, the third connecting part is a circular ring connected to the second connecting part, and the needle hole only needs to penetrate the first connecting part and the second connecting part from the outside to communicate with the cavity in the tube body, thereby facilitating the penetration of the needle hole.

[0009] Optionally, the through hole is arranged at a position of the tube body where the PBMC cell layer is located.

[0010] By adopting the above technical solution, since the PBMC cell layer is located in the middle region of the tube body after centrifugation of the peripheral blood sample, the PBMC cells can be extracted by the extraction member arranged on the through hole, thereby improving the purity of the PBMC.

[0011] Optionally, the tube body is made of polypropylene material.

[0012] By adopting the above technical solution, polypropylene is a kind of excellent thermoplastic synthetic resin, which is a colorless translucent thermoplastic lightweight general-purpose plastic, has chemical resistance, heat resistance, electrical insulation, high strength mechanical properties and good high wear resistance processing performance, etc. The polypropylene material used in the tube body in the present application can withstand at least 12000xg centrifugal force without breaking.

[0013] Optionally, the extraction member is made of medical butyl rubber plug material.

[0014] By adopting the above technical solution, the butyl rubber plug has good airtightness, good heat resistance, good acid and alkali resistance, and high internal cleanliness. Not only can it be stably fixed in the through hole, but also can increase the sealing property and reduce the risk of leakage of the peripheral blood sample in the tube body. In addition, the butyl rubber plug material can help the insertion of the needle, thereby helping the extraction of the PBMC cells.

[0015] One or more technical solutions provided in the present application have at least the following technical effects or advantages:

[0016] 1. The present application penetrates the first connecting part and the second connecting part of the extraction member through the needle hole to communicate with the cavity in the tube body, thereby realizing accurate absorption of the PBMC cell layer in the tube body, increasing the yield of PBMC, and improving the purity of PBMC.

[0017] 2、The polypropylene material is used as the material of the pipe body, and the pipe body has chemical resistance, heat resistance, electrical insulation, high-strength mechanical properties, good high-wear resistance and the like, can withstand centrifugal force of at least 12000xg without breaking, can withstand greater centrifugal force, and has better centrifugal effect.

[0018] 3、The medical butyl rubber plug material is used as the material of the extraction piece, which can be stably fixed in the through hole, increase the sealing property, reduce the risk of leakage of the peripheral blood sample in the pipe body, facilitate the insertion of the needle, and facilitate the extraction of the PBMC cells. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0020] Figure 1 It is a whole structure schematic view of the simple device for separating PBMC in the embodiment of the present application.

[0021] Figure 2 It is an exploded structure schematic view of the simple device for separating PBMC in the embodiment of the present application.

[0022] Figure 3 It is a whole structure schematic view of the extraction piece in the embodiment of the present application.

[0023] Figure 4 It is a plane structure schematic view of the simple device for separating PBMC in the embodiment of the present application.

[0024] Legend: 1, pipe body; 11, through hole; 2, extraction piece; 21, first connecting part; 22, second connecting part; 23, third connecting part.

[0025] a, plasma layer; b, PMBC layer; c, separation liquid layer; d, red blood cell and granulocyte layer. DETAILED DESCRIPTION

[0026] The present application provides a simple device for separating PBMC, which can increase the yield of PBMC, reduce the number of other mixed cells such as red blood cells, granulocytes and platelets, improve the purity of PBMC, and is more convenient to operate.

[0027] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0028] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-described drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or server including a series of steps or units does not have to be limited to only those steps or units clearly listed, but can include other steps or modules not clearly listed or inherent to the process, method, product or device.

[0029] With reference to Figure 1 , a simple device for separating PBMCs in the embodiment comprises a tube body 1 and an extraction piece 2 arranged on the side wall of the tube body 1.

[0030] With reference to Figure 2 , one side of the tube body 1 is provided with a through hole 11; one side of the through hole 11 communicates with the chamber in the tube body 1, and the other side of the through hole 11 communicates with the outside world, and the PBMC cells in the chamber of the tube body 1 are transferred to the outside world through the extraction piece 2.

[0031] In addition, with reference to Figure 3 , the extraction piece 2 comprises a first connecting part 21, a second connecting part 22 and a third connecting part 23 connected integrally, the first connecting part 21 is arranged in the tube body 1, the second connecting part 22 is clamped at the hole wall of the through hole 11, and the third connecting part 23 is arranged outside the tube body 1.

[0032] Among them, the third connecting part 23 can be a disc or a ring. In the embodiment of the present application, the third connecting part 23 is a sealing ring fixed on the second connecting part 22. Further, the needle hole only needs to penetrate the first connecting part 21 and the second connecting part 22 from the outside world, so as to realize communication with the chamber in the tube body.

[0033] Since the peripheral blood in the tube body 1 is stratified during centrifugation, various stratifications such as PBMC cell layer, red blood cell, granulocyte and platelet are formed. Specifically, with reference to Figure 4 , it is divided into plasma layer a, PMBC layer b, separation liquid layer c and red blood cell and granulocyte layer d.

[0034] Therefore, the extraction piece 2 penetrates the first connecting part 21 and the second connecting part 22 from the pinhole of the external environment to communicate with the chamber in the tube body 1, so as to accurately absorb the PBMC cell layer in the tube body 1, increase the yield of PBMC, improve the purity of PBMC, and make the operation more simple and convenient.

[0035] Further, the through hole 11 is arranged at the position of the PBMC cell layer in the tube body 1. Since the PBMC cell layer is located in the middle region of the tube body 1 after centrifugation of the peripheral blood sample, the PBMC cells can be extracted by the extraction piece 2 arranged on the through hole 11, so as to improve the purity of PBMC.

[0036] In addition, in the embodiment of the present application, the material of the tube body 1 is polypropylene. The polypropylene is a kind of excellent thermoplastic synthetic resin, which is a colorless and translucent thermoplastic light general-purpose plastic, has chemical resistance, heat resistance, electrical insulation, high strength mechanical properties and good high wear resistance processing performance, etc. The polypropylene material of the tube body 1 in the present application can withstand at least 12000xg centrifugal force without breaking.

[0037] In addition, the extraction piece 2 is made of medical butyl rubber plug material. The butyl rubber plug has good airtightness, heat resistance, acid and alkali resistance, and high internal cleanliness. It can not only be stably fixed in the through hole 11, but also can increase the sealing property and reduce the risk of leakage of the peripheral blood sample in the tube body 1. In addition, the butyl rubber plug material can help the insertion of the needle, and then help the extraction of PBMC cells.

[0038] In addition, it should be noted that the tube body 1 in the embodiment of the present application is a centrifuge tube with a volume of 50ml, and the diameter of the through hole 11 on the tube body 1 is 0.5cm, the diameter of the second connecting part 22 is 0.5cm, the diameter of the first connecting part 21 is 1.0cm, the third connecting part 23 is a circular ring with an outer diameter of 1.0cm and an inner diameter of 0.4cm. In addition, the hole depth of the through hole 11 is 0.1cm, and the thickness of the first connecting part 21, the second connecting part 22 and the third connecting part 23 is 0.1cm. By unifying the specifications of the tube body 1, the simple device for separating PBMC can be mass-produced, which helps to extract PBMC cells in high purity in different batches, and the operation is also more simple and convenient.

[0039] It should be noted that the above-mentioned embodiment sequences of the present application are merely for description only, but not for representing the advantages and disadvantages of the embodiments. And the above-mentioned embodiments of the present specification are described. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps recited in the claims can be performed in a different order than the order in which they are recited and still achieve desirable results. In addition, the processes depicted in the figures do not necessarily require the particular order shown, or sequential order, to achieve the desired results. In certain implementations, multitasking and parallel processing can be advantageous or necessary.

[0040] The above only describes the preferred embodiments of the present application, and does not limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

[0041] The specification and drawings are merely exemplary of the present application, and are considered to cover any and all modifications, variations, combinations or equivalents that fall within the scope of the present application. Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the scope of the present application. Thus, if these modifications and variations of the present application belong to the scope of the present application and its equivalent technology, the present application is intended to include these modifications and variations.

Claims

1. A simple device for isolating PBMCs, characterized in that, The utility model relates to a kind of extraction devices, including pipe body (1) and the extraction piece (2) being arranged on the side wall of the pipe body (1); One side of the pipe body (1) is provided with a through hole (11); one side of the through hole (11) communicates with the chamber in the pipe body (1), and the other side of the through hole (11) communicates with the outside world; The extraction piece (2) includes a first connecting part (21), a second connecting part (22) and a third connecting part (23) connected integrally, the first connecting part (21) is arranged in the pipe body (1), the second connecting part (22) is clamped at the hole wall of the through hole (11), and the third connecting part (23) is arranged outside the pipe body (1); The extraction piece (2) is pierced from the outside world and penetrates the chamber in the pipe body (1) communicated with the extraction piece (2).

2. The simple device for isolating PBMC according to claim 1, wherein, The third connecting part (23) is a circular ring connected to the second connecting part (22), and the extraction piece (2) is pierced from the outside world and penetrates the first connecting part (21) and the second connecting part (22) and communicates with the chamber in the pipe body (1).

3. The simple device for isolating PBMC according to claim 2, wherein, The through hole (11) is arranged at the position of PBMC cell layer of the pipe body (1).

4. The simple device for isolating PBMC according to claim 1, wherein, The pipe body (1) is made of polypropylene material.

5. The simple device for isolating PBMC according to claim 1, wherein, The extraction piece (2) is made of medical butyl rubber plug material.

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