Double-layer culture medium plate preparation device

Abstract

The utility model discloses a double-layer culture medium plate preparation device in the technical field of plate culture medium preparation, which comprises a base, a rotating disc is rotatably connected to the top of the base, and an auxiliary mechanism is arranged at the bottom of the rotating disc. Then a bevel gear II and the cam are driven to rotate by utilizing a motor IV and a bevel gear I, so that agar and a sample solution are fully mixed, the workload of manually rotating the plates is reduced, the rotating disc drives the limiting piece to switch positions, the plates can be treated one by one, the consumed time is shortened, and the working efficiency is improved. The positions of the two liquid adding pipes can be switched, a threaded rod is used for driving a connecting block and a piston to move downwards, discharging of liquid in the liquid adding pipes can be controlled, therefore, filling of two different kinds of liquid in the plate is achieved, and overall operation is convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to the flat plate culture medium preparation technical field, concretely to a double -deck culture medium flat plate preparation device. BACKGROUND

[0002] The culture flat plate culture medium is the most commonly used solid culture medium form for obtaining pure culture of microorganism, and it is the solid flat plane of the solid culture medium after cooling and solidification in a sterile culture dish, which is often referred to as a culture flat plate.

[0003] In the prior art, after pouring the liquid agar into the flat dish, the flat dish needs to be rotated to fully mix the culture medium with the sample solution, and when the number of flat dishes is large, the work load of rotating the flat dishes one by one by hand is large, and it takes a long time, which reduces the efficiency of flat plate preparation. Based on this, the utility model designs a double-layer culture medium flat plate preparation device to solve the above problems. UTILITY MODEL CONTENTS

[0004] The utility model discloses a double -deck culture medium flat plate preparation device aims at providing a double -deck culture medium flat plate preparation device to solve the problem of the liquid agar pouring in the flat dish, and the flat dish needs to be rotated to fully mix the culture medium with the sample solution, and when the number of flat dishes is large, the work load of rotating the flat dishes one by one by hand is large, and it takes a long time, which reduces the efficiency of flat plate preparation.

[0005] To achieve the above object, the utility model provides the following technical scheme: a double -deck culture medium flat plate preparation device, including the base, the base top rotation is connected with the rotary disc, the rotary disc bottom is provided with the auxiliary mechanism, the auxiliary mechanism includes the hydraulic cylinder, the cam and the support plate, the hydraulic cylinder bottom is fixedly connected with the base inner chamber bottom, the hydraulic cylinder top is fixedly connected with the auxiliary frame, the auxiliary frame top is rotatably connected with the cam, the cam top is fixedly connected with the connecting shaft, the connecting shaft top penetrates the rotary disc back and is inserted into the limiting piece, the limiting piece bottom is clamped with the rotary disc, and the cam one side is overlapped with the auxiliary wheel, the auxiliary wheel top is rotatably connected with the guide, the guide bottom is rotatably connected with the support frame.

[0006] Preferably, the cam bottom is fixedly connected with bevel gear no. 2, one side of bevel gear no. 2 is engaged with bevel gear no. 1, one side of bevel gear no. 1 is fixedly connected with the output shaft of motor no. 4, and the bottom of motor no. 4 is fixedly connected with the auxiliary frame.

[0007] Preferably, the bottom of the base inner chamber is fixedly connected with a fixed plate, the top of the fixed plate is fixedly connected with motor no. 3 and a speed reducer, and the output shaft of motor no. 3 and one side of the speed reducer are provided with a sprocket assembly.

[0008] Preferably, the output shaft at the top of the speed reducer is fixedly connected with the rotary disc.

[0009] Preferably, one side of the base is fixedly connected with an extension plate, a motor one is embedded in the base and the extension plate, and a flat gear one is fixedly connected to the top of the motor one.

[0010] Preferably, the flat gear one is rotatably connected between the bottom of the flat gear one and the extension plate, a flat gear two is engaged with one side of the flat gear one, a connecting rod is inserted through the inside of the flat gear two, the connecting rod is rotatably connected between the bottom of the connecting rod and the base, and a support frame is fixedly connected to the top of the connecting rod.

[0011] Preferably, a motor two is fixedly connected to the top of the support frame, a threaded rod is fixedly connected to one end of an output shaft of the motor two, a connecting block is threadedly connected to the threaded rod, and the connecting block is slidably connected to one side of the support frame.

[0012] Preferably, a support block is fixedly connected to one side of the support frame, a liquid adding pipe is connected to the bottom of the support block through bolts, and a piston is slidably connected in the liquid adding pipe.

[0013] Preferably, the connecting block is fixedly connected to the bottom of the piston, and the piston is slidably connected between the top of the piston and the support block.

[0014] Compared with the prior art, the present utility model has the beneficial effects that:

[0015] (1) The present utility model adjusts the use height of the hydraulic cylinder adjusting cam and the auxiliary frame, inserts the connecting shaft and the bottom of the limiting piece, connects the cam and the limiting piece, drives the bevel gear two and the cam to rotate by the motor four and the bevel gear one, makes the agar and the sample liquid fully mix, reduces the work load of manually rotating the flat plate, drives the limiting piece to switch positions by the rotating disc, can process the flat plates one by one, shortens the time consumption, improves the efficiency when preparing the flat plate, saves the experimental steps, combines two steps into one step, and reduces the heat loss.

[0016] (2) The rotation of the flat gear one is driven by the motor one and the flat gear one, drives the flat gear two and the connecting rod to rotate on the top of the base, and then switches the positions of the two liquid adding pipes, drives the connecting block and the piston to move downward by the threaded rod, controls the discharge of the liquid in the liquid adding pipe, and then realizes the injection of two different liquids in the flat plate, and the whole operation is relatively convenient. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical scheme of the embodiments of the present utility model, the following will briefly introduce the drawings needed to be used in the embodiment description, and obviously, the drawings in the following description are only some embodiments of the present utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creating labor.

[0018] Figure 1 It is the structure three-dimensional view of the utility model;

[0019] Figure 2 It is the structure meshing view of the utility model's gear one and gear two;

[0020] Figure 3 It is the structure installation view of the utility model's motor three;

[0021] Figure 4 It is the structure connection view of the utility model's reduction gearbox and rotating disc;

[0022] Figure 5 It is the structure connection view of the utility model's limiting piece and cam;

[0023] Figure 6 It is the structure meshing view of the utility model's bevel gear one and bevel gear two.

[0024] In the drawing, the component list represented by each sign is as follows:

[0025] 1-support frame, 2-rotating disc, 3-limiting piece, 4-base, 5-extension plate, 6-sprocket assembly, 7-gear one, 8-motor one, 9-gear two, 10-connecting rod, 11-motor two, 12-threaded rod, 13-connecting block, 14-liquid adding pipe, 15-motor three, 16-fixed plate, 17-reduction gearbox, 18-hydraulic cylinder, 19-cam, 20-assistant frame, 21-supporting plate, 22-connecting shaft, 23-assistant wheel, 24-guiding piece, 25-motor four, 26-bevel gear one, 27-bevel gear two. DETAILED DESCRIPTION

[0026] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor fall within the protection scope of the utility model.

[0027] Please refer to Figures 1-6The utility model provides a technical scheme: a double -layer culture medium flat board preparation device, including base 4, base 4 top rotatory connection has rotary disc 2, rotary disc 2 bottom is provided with auxiliary mechanism, and auxiliary mechanism includes hydraulic cylinder 18, cam 19 and support plate 21, and the bottom of hydraulic cylinder 18 is fixedly connected with the bottom of the inner chamber of base 4, and the top of hydraulic cylinder 18 is fixedly connected with auxiliary frame 20, and the top of auxiliary frame 20 is rotatoryly connected with cam 19, and the top of cam 19 is fixedly connected with connecting shaft 22, and connecting shaft 22 top penetrates rotary disc 2 rear and inserts into and connects limit piece 3, and the bottom of limit piece 3 is clamped with rotary disc 2, and cam 19 side is overlapped with auxiliary wheel 23, and the top of auxiliary wheel 23 is rotatoryly connected with guide 24, and the bottom of guide 24 is rotatoryly connected with support frame 1.

[0028] The bottom of cam 19 is fixedly connected with bevel gear no. 2 27, and bevel gear no. 2 27 side is engaged with bevel gear no. 1 26, and bevel gear no. 1 26 side is fixedly connected with the output shaft of motor no. 4 25, and the bottom of motor no. 4 25 is fixedly connected with auxiliary frame 20, and the bottom of the inner chamber of base 4 is fixedly connected with fixed plate 16, and the top of fixed plate 16 is fixedly connected with motor no. 3 15 and reduction box 17, and the output shaft of motor no. 3 15 and reduction box 17 side are provided with sprocket wheel subassembly 6, and the output shaft of reduction box 17 top is fixedly connected with rotary disc 2.

[0029] Wherein, utilize base 4 can be for rotary disc 2 bottom support, through hydraulic cylinder 18 can adjust the use height of cam 19 and auxiliary frame 20, further control connecting shaft 22 and the connection or separation of limit piece 3, auxiliary frame 20 can be for the installation of motor no. 4 25 and support plate 21 support, thereby facilitating support plate 21 for the rotation of guide 24 support, motor no. 4 25 drive bevel gear no. 1 26 rotation, make bevel gear no. 2 27 and cam 19 synchronous rotation, further realize limit piece 3 on rotary disc 2 top rotation, the mixing of agar and sample liquid in the inside of auxiliary flat dish, when cam 19 rotates, auxiliary wheel 23 can contact with the side of cam 19, through guide 24 can drive auxiliary wheel 23 on the position adjustment of support plate 21 top, thereby reducing the motion interference of auxiliary wheel 23 to cam 19;

[0030] Meanwhile, utilize fixed plate 16 can be for the bottom support and reinforcement of motor no. 3 15 and reduction box 17, and sprocket wheel subassembly 6 can assist the transmission between motor no. 3 15 and reduction box 17, further utilize motor no. 3 15 as the power source of reduction box 17 operation, drive the rotation of gear set in reduction box 17, thereby drive rotary disc 2 on the top of reduction box 17 rotation, change the position of multiple limit piece 3, realize the position switching of flat dish.

[0031] Please refer to Figures 1-3The bottom base 4 is fixedly connected with an extension plate 5 on one side, a motor one 8 is embedded in the bottom base 4 and the extension plate 5, a flat gear one 7 is fixedly connected to the top of the motor one 8, the flat gear one 7 is rotatably connected with the extension plate 5 at the bottom, a flat gear two 9 is engaged on one side of the flat gear one 7, a connecting rod 10 is inserted through the inside of the flat gear two 9, the connecting rod 10 is rotatably connected with the bottom base 4 at the bottom, a support frame 1 is fixedly connected to the top of the connecting rod 10, a motor two 11 is fixedly connected to the top of the support frame 1, a threaded rod 12 is fixedly connected to one end of the output shaft of the motor two 11, a connecting block 13 is threadedly connected to the threaded rod 12, the connecting block 13 is slidably connected with the support frame 1 on one side, a support block is fixedly connected to one side of the support frame 1, a liquid adding pipe 14 is connected to the bottom of the support block through bolts, a piston is slidably connected in the liquid adding pipe 14, the connecting block 13 is fixedly connected with the piston at the bottom, and the piston is slidably connected between the support block and the support block at the top.

[0032] The extension plate 5 can support and reinforce one side of the bottom base 4, the bottom base 4 and the extension plate 5 can wrap and protect the outside of the motor one 8, the motor one 8 can provide power for the rotation of the flat gear one 7, and then drive the flat gear two 9 and the connecting rod 10 to rotate, the bottom base 4 can support the bottom of the connecting rod 10 when the connecting rod 10 rotates, thereby improving the stability of the rotation of the connecting rod 10, quickly realizing the position switching between the two liquid adding pipes 14, thereby injecting different liquids into the flat plate, the support frame 1 can support the bottom of the motor two 11, the support frame 1 can guide the movement of the connecting block 13 when the threaded rod 12 drives the connecting block 13 to rotate, the connecting block 13 is fixedly connected with the piston, facilitating the synchronous movement of the piston and the connecting block 13, thereby assisting the suction or discharge of the liquid in the liquid adding pipe 14, and the support block can reinforce the top of the liquid adding pipe 14, improving the stability of the liquid adding pipe 14 during use.

[0033] The working principle of the whole is that first two liquid filling pipes 14 respectively hold different liquids, one of which holds liquid agar, a culture dish is placed in the limiting piece 3, then the threaded rod 12 is driven to rotate by the motor two 11, the threaded rod 12 drives the connecting block 13 and the piston to move downward, and a certain amount of agar is discharged into the flat plate, then the auxiliary frame 20 and the cam 19 are driven to move upward by the hydraulic cylinder 18, the connecting shaft 22 at the top of the cam 19 is inserted into the bottom of the limiting piece 3, then the bevel gear one 26 is driven to rotate by the motor four 25, the bevel gear one 26 is engaged with the bevel gear two 27, and the bevel gear two 27 drives the cam 19 to rotate synchronously, the auxiliary wheel 23 and the guide piece 24 are driven to rotate with the guide piece 24 as the center at the connection with the support plate 21 when the cam 19 rotates, the limiting piece 3 and the flat plate are rotated on the top of the rotating disc 2 by the cam 19, so that the agar in the flat plate is uniformly mixed with the sample, after rotation, the cam 19 and the auxiliary frame 20 are driven to move downward by the hydraulic cylinder 18, the connecting shaft 22 is separated from the limiting piece 3, then the gear set in the reduction box 17 is driven to work by the motor three 15, so that the rotating disc 2 is rotated on the top of the base 4 by the gear set in the reduction box 17, the position of the flat plate is changed, the next flat plate is moved under the liquid filling pipe 14, and the agar is injected into the inside of the multiple flat plates in turn until all the flat plates are injected, the initial agar is solidified in the flat plate, then the flat gear one 7 is driven to rotate by the motor one 8, the flat gear one 7 is rotated with the flat gear two 9, the connecting rod 10 and the support frame 1 are driven to rotate, the positions of the two liquid filling pipes 14 are switched, the VRBA-MUG is injected into the flat plate through the liquid filling pipe 14 in turn, the surface layer of the flat plate is covered, and after solidification, the flat plate is taken out and turned over and placed in the culture equipment for culture, the liquid agar therein is crystal violet neutral red bile salt agar, the above process saves the experimental steps, combines two steps into one step, saves time, improves efficiency, and reduces heat loss.

[0034] In the description of the present specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0035] The preferred embodiments disclosed above are only used to help describe the utility model. The preferred embodiments do not describe all the details and do not limit the utility model to the specific embodiments. Obviously, according to the content of the specification, many modifications and changes can be made. The specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that the persons skilled in the art can well understand and utilize the utility model. The utility model is limited by the claims and the entire scope and equivalents thereof.

Claims

1. A device for preparing double-layer culture medium plates, comprising a base (4) and a rotating disc (2) rotatably connected to the top of the base (4), characterized in that: The rotating disc (2) bottom is provided with an auxiliary mechanism; The auxiliary mechanism includes a hydraulic cylinder (18), a cam (19) and a support plate (21), the bottom of the hydraulic cylinder (18) is fixedly connected with the bottom of the inner cavity of the base (4), and the top of the hydraulic cylinder (18) is fixedly connected with an auxiliary frame (20); The top of the auxiliary frame (20) is rotatably connected with the cam (19), the top of the cam (19) is fixedly connected with a connecting shaft (22), and the top of the connecting shaft (22) penetrates the rear of the rotating disc (2) and is inserted into a limiting piece (3); The bottom of the limiting piece (3) is clamped with the rotating disc (2), one side of the cam (19) is overlapped with an auxiliary wheel (23), the top of the auxiliary wheel (23) is rotatably connected with a guide piece (24), and the bottom of the guide piece (24) is rotatably connected with the support frame (1).

2. The dual layer culture medium plate preparation apparatus according to claim 1, characterized by: The bottom of the cam (19) is fixedly connected with a bevel gear two (27), one side of the bevel gear two (27) is engaged with a bevel gear one (26), one side of the bevel gear one (26) is fixedly connected with the output shaft of a motor four (25), and the bottom of the motor four (25) is fixedly connected with the auxiliary frame (20).

3. The dual layer culture medium plate preparation apparatus according to claim 1, characterized by: The bottom of the base (4) is fixedly connected with a fixed plate (16), the top of the fixed plate (16) is fixedly connected with a motor three (15) and a speed reducer (17), and the output shaft of the motor three (15) and one side of the speed reducer (17) are provided with a sprocket assembly (6).

4. The dual-layer culture medium plate preparation apparatus according to claim 3, wherein: The output shaft at the top of the speed reducer (17) is fixedly connected with the rotating disc (2).

5. The dual-layer culture medium plate preparation apparatus according to claim 1, wherein: One side of the base (4) is fixedly connected with an extension plate (5), the base (4) and the extension plate (5) are embedded with a motor one (8), and the top of the motor one (8) is fixedly connected with a spur gear one (7).

6. The dual-layer culture medium plate preparation apparatus according to claim 5, wherein: The bottom of the spur gear one (7) is rotatably connected with the extension plate (5), one side of the spur gear one (7) is engaged with a spur gear two (9), the inside of the spur gear two (9) is inserted and provided with a connecting rod (10), the bottom of the connecting rod (10) is rotatably connected with the base (4), and the top of the connecting rod (10) is fixedly connected with the support frame (1).

7. The dual-layer culture medium plate preparation apparatus according to claim 6, wherein: The top of the support frame (1) is fixedly connected with a motor two (11), one end of the output shaft of the motor two (11) is fixedly connected with a threaded rod (12), the threaded rod (12) is connected with a connecting block (13) through threads, and one side of the connecting block (13) is slidably connected with the support frame (1).

8. The dual-layer culture medium plate preparation apparatus according to claim 7, wherein: One side of the support frame (1) is fixedly connected with a support block, the bottom of the support block is connected with a liquid adding pipe (14) through bolts, and the inside of the liquid adding pipe (14) is slidably connected with a piston.

9. The dual-layer culture medium plate preparation apparatus according to claim 8, characterized by: The bottom of the connecting block (13) is fixedly connected with the piston, and the top of the piston is slidably connected between the support block and the support block.

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