Efficient separation culture dish for mycobacterium strains
By designing a highly efficient separation culture dish, the problems of insufficient simulated growth conditions and contamination in traditional culture dishes are solved, enabling rapid isolation and accurate culture of mycobacteria, adapting to diverse growth needs, and preventing the invasion of contaminating bacteria.
Patent Information
- Application Number
- CN202423001725.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-06
- Publication Date
- 2025-11-18
- Estimated Expiration
- 2034-12-06
AI Technical Summary
Traditional culture dishes cannot effectively simulate the growth conditions of mycobacteria in the natural environment, resulting in slow growth, low isolation efficiency, and susceptibility to external contamination, making it difficult to meet the culture requirements of mycobacteria with special growth characteristics.
A highly efficient isolation culture dish for mycobacterial strains was designed. It is made of transparent medical-grade plastic and is equipped with a separation tank, nutrient delivery channel and gas storage chamber. It is also equipped with adsorbed silica gel and gas diffusion membrane, combined with a contamination monitoring layer to simulate natural environmental growth conditions and prevent the invasion of other bacteria.
It improves the growth rate and isolation efficiency of mycobacteria, prevents external contamination, can simulate diverse growth requirements, provides initial attachment sites and nutrient sources, monitors contamination in a timely manner, and ensures the accuracy of the culture process.
Smart Images
Figure CN223561576U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to a culture dish technical field especially relates to a high -efficient separation culture dish of mycobacterium species. BACKGROUND
[0002] Mycobacterium has important significance in the research of medical science, microbiology and other fields, for example, mycobacterium tuberculosis is closely related to a variety of diseases. However, when the mycobacterium species is isolated and cultured, the traditional culture dish has many problems. The structure of the ordinary culture dish is relatively simple, which cannot effectively simulate the growth conditions of mycobacterium in the natural environment, resulting in slow growth and low separation efficiency. Moreover, the traditional culture dish is easily polluted by the outside world, and it is difficult to avoid the invasion of miscellaneous bacteria during the culture process, which affects the accurate separation and identification of mycobacterium species. In addition, for some special mycobacterium with special growth characteristics, such as specific gas environment, nutrient concentration gradient, etc., the existing culture dish cannot meet the diversified culture needs, which limits the in-depth research and analysis of mycobacterium species
[0003] When using, the structure of the ordinary culture dish is relatively simple, which cannot effectively simulate the growth conditions of mycobacterium in the natural environment, resulting in slow growth and low separation efficiency; the traditional culture dish is easily polluted by the outside world, and it is difficult to avoid the invasion of miscellaneous bacteria during the culture process; therefore we propose a high -efficient separation culture dish of mycobacterium species. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a high -efficient separation culture dish of mycobacterium species, which solves the existing problems.
[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:
[0006] A high -efficient separation culture dish of mycobacterium species, including the bacterial body culture dish main part, the top of bacterial body culture dish main part is provided with the upper cover, the bottom of bacterial body culture dish main part is equipped with a plurality of separation grooves, the one side of separation groove is equipped with the nutrition delivery channel, the bottom inner wall of bacterial body culture dish main part is fixedly installed with the round convex block, the outside of round convex block is fixedly installed with the adsorption silica gel.
[0007] As a further improvement of the above scheme, the top of upper cover is fixedly installed with gas storage cavity, one side of gas storage cavity is fixedly installed with pump, one side of pump is fixedly installed with gas transmission pipeline, the top inner wall of upper cover is fixedly installed with gas diffusion membrane, one end of gas transmission pipeline is fixedly installed at the top of gas diffusion membrane, the outside of gas transmission pipeline is fixedly installed with solenoid valve.
[0008] As a further improvement of the above-mentioned scheme, the connecting part of the bacterial culture dish main body and the upper cover is provided with a pollution monitoring layer, and the outer side of the bacterial culture dish main body and the inner side of the upper cover are both provided with threads.
[0009] As a further improvement of the above-mentioned scheme, the top of the upper cover is provided with a through hole, and the gas transmission pipeline is fixedly installed on the inner side of the through hole.
[0010] As a further improvement of the above-mentioned scheme, the pollution monitoring layer is made of a color-changing indicating material, and a specific enzyme film is arranged above the pollution monitoring layer.
[0011] By adopting the above technical scheme, specific nutrients or growth factors can be adsorbed, initial attachment sites and nutrient sources are provided for the growth of mycobacteria, and the nutrient delivery channels can control the exchange of substances between different separation tanks and the circular protruding blocks.
[0012] As a further improvement of the above-mentioned scheme, the gas diffusion membrane adopts a microporous structure, and the adsorbed silica gel is made of silica gel material.
[0013] By adopting the above technical scheme, the gas storage cavity can store different kinds of gases, such as oxygen and carbon dioxide, and the gases can be adjusted according to the growth needs of mycobacteria; the gas diffusion membrane adopts a microporous structure, which can make the gas slowly and uniformly diffuse to each area inside the culture dish.
[0014] As a further improvement of the above-mentioned scheme, the outer side of the adsorbed silica gel is provided with high-concentration mycobacterium special culture medium components, and the inner side of the gas storage cavity stores gases.
[0015] By adopting the above technical scheme, high-concentration mycobacterium special culture medium components are arranged above the circular protruding blocks and the adsorbed silica gel, and as the nutrient delivery channels extend upwards to the separation tanks, the concentration of nutrients gradually decreases; in this way, the growth process of mycobacteria from a rich nutrient area to a poor nutrient area in a natural environment can be simulated, which is beneficial to the separation and culture of mycobacteria strains at different growth stages and growth speeds.
[0016] Compared with the prior art, the beneficial effects of the present application are as follows:
[0017] (1) The high-efficiency separation and culture dish for mycobacterium species is made of transparent medical-grade plastic material, has good chemical stability and biocompatibility, and is convenient for observing the culture process; the bacterial culture dish main body and the upper cover are connected through threads, have good sealing performance, and can effectively prevent external bacteria pollution; the upper surface of the circular protrusion has a layer of adsorbed silica gel, which can adsorb specific nutrients or growth factors, provide initial attachment sites and nutrient sources for the growth of mycobacteria;
[0018] (2) The present invention provides a high-efficiency isolation culture dish for mycobacterial strains, wherein a contamination monitoring layer is set at the seal between the main body of the culture dish and the top cover; the contamination monitoring layer uses a color-changing indicator material, such as a film containing specific enzymes or chemicals; when the culture dish is contaminated by other bacteria, the metabolic products or enzymes produced by the other bacteria will react with the indicator material of the contamination monitoring layer, causing the indicator material to change color, thereby intuitively reminding researchers that the culture dish has been contaminated and taking timely measures. Attached Figure Description
[0019] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0020] Figure 1 This is a three-dimensional structural diagram of the present invention;
[0021] Figure 2 This is a partial three-dimensional structural diagram of the present utility model;
[0022] Figure 3 This is a three-dimensional structural diagram of the cross-section of this utility model;
[0023] Figure 4 This is a three-dimensional structural diagram of the present invention viewed from below.
[0024] In the diagram: 1. Main body of the bacterial culture dish; 2. Top cover; 3. Separation tank; 4. Nutrient delivery channel; 5. Circular protrusion; 6. Adsorbed silica gel; 7. Gas storage chamber; 8. Pump; 9. Gas diffusion membrane; 10. Gas transmission pipeline; 11. Solenoid valve; 12. Contamination monitoring layer. Detailed Implementation
[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.
[0026] refer to Figures 1-4The utility model relates to a high -efficient separation culture dish of mycobacterium species, including bacteria culture dish main part 1, the top of bacteria culture dish main part 1 is provided with upper cover 2, the bottom of bacteria culture dish main part 1 is set up with a plurality of separation groove 3, the one side of separation groove 3 is set up with the nutrition delivery channel 4, the bottom inner wall of bacteria culture dish main part 1 is fixedly installed with round convex block 5, the outside of round convex block 5 is fixedly installed with adsorption silica gel 6.
[0027] In the embodiment, the top of the upper cover 2 is fixedly installed with a gas storage cavity 7, one side of the gas storage cavity 7 is fixedly installed with a pump 8, one side of the pump 8 is fixedly installed with a gas transmission pipeline 10, the top inner wall of the upper cover 2 is fixedly installed with a gas diffusion membrane 9, one end of the gas transmission pipeline 10 is fixedly installed on the top of the gas diffusion membrane 9, and the outer side of the gas transmission pipeline 10 is fixedly installed with a solenoid valve 11.
[0028] In the embodiment, the connection between the bacteria culture dish main part 1 and the upper cover 2 is provided with a pollution monitoring layer 12, and the outer side of the bacteria culture dish main part 1 and the inner side of the upper cover 2 are both provided with threads.
[0029] In the embodiment, the top of the upper cover 2 is provided with a through hole, and the gas transmission pipeline 10 is fixedly installed on the inner side of the through hole.
[0030] In the embodiment, the pollution monitoring layer 12 is made of a color-changing indicating material, and a specific enzyme film is arranged above the pollution monitoring layer 12.
[0031] In the embodiment, the gas diffusion membrane 9 adopts a microporous structure, and the adsorption silica gel 6 is made of silica gel material.
[0032] In the embodiment, the outer side of the adsorption silica gel 6 is provided with a high-concentration mycobacterium special culture medium component, and the inner side of the gas storage cavity 7 stores gas.
[0033] The implementation principle of the high-efficiency separation and culture dish for mycobacterium species in the embodiment of the present application is as follows: the bacteria culture dish main body 1 is made of transparent medical-grade plastic material, has good chemical stability and biological compatibility, and is convenient for observing the culture process; the bacteria culture dish main body 1 and the upper cover 2 are connected through threads, have good sealing performance, and can effectively prevent external miscellaneous bacteria from polluting; the bacteria culture dish main body 1 is internally provided with a plurality of separation grooves 3, the separation grooves 3 are annularly distributed, a small nutrient delivery channel 4 is arranged between each separation groove 3, a circular protrusion is arranged at the center position of the inner wall of the bottom of the bacteria culture dish main body 1, the upper surface of the circular protrusion is provided with a layer of adsorbed silica gel 6, and the adsorbed silica gel 6 can adsorb specific nutrients or growth factors, thereby providing initial adhesion sites and nutrient sources for the growth of mycobacterium; the nutrient delivery channels 4 can control the exchange of substances between the different separation grooves 3 and the circular protrusion block 5; the gas storage cavity 7 can store different kinds of gases, such as oxygen and carbon dioxide, and the gases can be adjusted according to the growth needs of mycobacterium; the gas diffusion membrane 9 adopts a microporous structure and can make the gases slowly and uniformly diffuse to each area inside the culture dish; the electromagnetic valve 11 can accurately control the release speed and flow of the gases, for example, for some mycobacterium that needs to grow in a low-oxygen environment, the release amount of oxygen can be reduced and the concentration of carbon dioxide can be increased by adjusting the valve; high-concentration mycobacterium special culture medium components are arranged above the circular protrusion block 5 and the adsorbed silica gel 6, the concentration of nutrients gradually decreases along with the extension of the nutrient delivery channels 4 to the upper part of the separation grooves 3; in this way, the growth process of mycobacterium from a rich-nutrient area to a poor-nutrient area in a natural environment can be simulated, which is beneficial to the separation and culture of mycobacterium species in different growth stages and growth speeds; the pollution monitoring layer 12 is arranged at the sealing position between the bacteria culture dish main body 1 and the upper cover 2; the pollution monitoring layer 12 adopts a color-changing indicating material, such as a film containing specific enzymes or chemical substances; when miscellaneous bacteria pollute the culture dish, the metabolic products or enzymes produced by the miscellaneous bacteria will react with the indicating material of the pollution monitoring layer, so that the indicating material changes color, thereby directly reminding researchers that the culture dish has been polluted, and timely measures can be taken.
[0034] In the description of the present specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0035] The above has carried out the detailed introduction to the high-efficiency separation culture dish of mycobacterium species provided by the utility model. The principle and implementation mode of the utility model are described by the specific examples in this paper, and the above example is only used for helping understanding the method and core idea of the utility model. It should be pointed out that for ordinary skilled person in the art, without departing from the principle of the utility model, the utility model can be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the utility model claim.
Claims
1. A high efficiency isolation plate for mycobacterial species, characterized in that, Include: The fungus culture dish main part (1), the top of fungus culture dish main part (1) is provided with upper cover (2), the bottom of fungus culture dish main part (1) is opened with multiple separation grooves (3), one side of separation groove (3) is opened with nutrition delivery channel (4), the inner wall of bottom of fungus culture dish main part (1) is fixedly installed with circular convex block (5), the outside of circular convex block (5) is fixedly installed with adsorption silica gel (6).
2. A high efficiency isolation plate for mycobacterial species according to claim 1, wherein, The top of upper cover (2) is fixedly installed with gas storage cavity (7), one side of gas storage cavity (7) is fixedly installed with pump (8), one side of pump (8) is fixedly installed with gas transmission pipeline (10), the inner wall of top of upper cover (2) is fixedly installed with gas diffusion membrane (9), one end of gas transmission pipeline (10) is fixedly installed in the top of gas diffusion membrane (9), the outside of gas transmission pipeline (10) is fixedly installed with electromagnetic valve (11).
3. The high efficiency isolation culture plate for mycobacterial species according to claim 1, wherein, The connecting place of fungus culture dish main part (1) and upper cover (2) is provided with pollution monitoring layer (12), the outside of fungus culture dish main part (1) and the inside of upper cover (2) are opened with thread.
4. The high efficiency isolation culture dish for mycobacterial species as claimed in claim 2, wherein, The top of upper cover (2) is opened with through hole, and the inside of through hole is fixedly installed with gas transmission pipeline (10).
5. The high efficiency isolation culture dish for mycobacterial species as claimed in claim 3, wherein, The material of pollution monitoring layer (12) is to use discoloration indicating material, and the upper of pollution monitoring layer (12) is provided with specific enzyme film.
6. The high efficiency isolation petri dish for mycobacterial species as claimed in claim 2, wherein, The gas diffusion membrane (9) adopts microporous structure, and the material of adsorption silica gel (6) is silica gel material.
7. The high efficiency isolation petri dish for mycobacterial species as claimed in claim 2, wherein, The outside of adsorption silica gel (6) is provided with high concentration of mycobacterium special culture medium component, and the inside of gas storage cavity (7) stores gas.