Heating incubation device

Abstract

The utility model belongs to the technical field of incubation, and discloses a heating incubation device which comprises a shell and a heating assembly, the shell defines a mounting cavity, the heating assembly is arranged in the mounting cavity, the heating assembly comprises a heat insulation part, a heating part and a metal heat conduction part, the heating part is arranged on the heat insulation part, the metal heat conduction part is arranged on the heating part, and the metal heat conduction part is arranged on the heat insulation part. And the support is used for supporting the cell culture container. According to the heating incubation device, the cell culture container is heated in a metal direct heating manner, so that the heating time of liquid in the cell culture container can be shortened, and the liquid can reach the target temperature more quickly and uniformly.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field especially, it relates to a heating incubation device. BACKGROUND

[0002] For cell culture equipment, especially the equipment for culturing stem cells, cells are very sensitive to temperature environment, and most of the cell culture equipment on the market has no incubation position in the environment of cell liquid change in addition to having the functions of cell culture and incubation in the cell culture box. In this case, when the cell culture container performs subculture and other actions, the cell culture container must frequently enter and exit the culture box, which will cause carbon dioxide leakage and fluctuation of the cell culture box, affecting the growth of cells in the culture box. In addition, since the cell culture box is air bath heating (heat is transferred through air), and the culture containers are plastic polymers with a thickness of not less than 2mm, the heat transfer efficiency is lower. SUMMARY

[0003] The utility model discloses a heating incubation device, which uses a metal direct heating method to heat the cell culture container, can shorten the heating time of the liquid in the cell culture container, and makes the liquid reach the target temperature faster and uniformly.

[0004] To achieve this purpose, the utility model adopts the following technical scheme:

[0005] The utility model discloses a heating incubation device, which uses a metal direct heating method to heat the cell culture container, can shorten the heating time of the liquid in the cell culture container, and makes the liquid reach the target temperature faster and uniformly.

[0006] In some embodiments, the heating assembly further comprises a temperature sensor for detecting the temperature of the heating element.

[0007] In some embodiments, the heating assembly further comprises a temperature protection switch electrically connected to the heating element and used for disconnecting the circuit of the heating element when the temperature of the heating element is too high.

[0008] In some embodiments, the metal heat-conducting piece is provided with a plurality of limiting portions, and the plurality of limiting portions are respectively stopped on different side walls of the cell culture container to limit the cell culture container on the metal heat-conducting piece.

[0009] In some embodiments, the metal heat-conducting piece has a positioning groove matched with the outer contour of the cell culture container.

[0010] In some embodiments, the heat insulation member has a mounting groove, and the heating member and the metal heat conducting member are both mounted in the mounting groove.

[0011] In some specific embodiments, a first mounting hole is arranged on a bottom wall of the mounting groove, a second mounting hole is arranged on the heating member, and a third mounting hole is arranged on the metal heat conducting member; wherein a connecting member is inserted into the third mounting hole through the first mounting hole and the second mounting hole in sequence, so as to connect the heat insulation member, the heating member and the metal heat conducting member.

[0012] In some embodiments, the heating assembly is multiple groups, and multiple groups of the heating assembly are arranged in the mounting cavity in a horizontal direction, and each group of the heating assembly includes multiple heating assemblies arranged in a vertical direction.

[0013] In some specific embodiments, each of the heating assemblies is provided with a supporting hole, and a supporting column is arranged between two heating assemblies arranged in a vertical direction in each group of the heating assemblies, and two ends of the supporting column are respectively inserted into the supporting holes of the two adjacent heating assemblies.

[0014] In some embodiments, the shell is further provided with a wire passing hole corresponding to the heating assembly, and used for leading out a cable of the heating assembly.

[0015] The heating incubation device has the following beneficial effects: in the actual working process, when the cell culture container is placed on the metal heat conducting member, the heat generated by the heating member can be transmitted to the cell culture container through the metal heat conducting member, the cell culture container is heated in the direct heating mode of the metal heat conducting member, the temperature fluctuation of the cell growth environment in the digestion process and the termination of the digestion process is reduced, the uniformity of the liquid in the cell culture container is effectively improved, and the consistency of the liquid temperature field is improved. And due to the existence of the heat insulation member, the heat generated by the heating member is less transmitted to the environment through the heat insulation member, so that the heat loss of the heating member can be effectively reduced, and the demand of the equipment for heating power is reduced. In addition, the heating incubation device is also provided with a windproof shell, and the shell is used for reducing the influence of the flowing wind in the safety cabinet on the incubation temperature.

[0016] The additional aspects and advantages of the present application will be partially given in the following description, partially will become obvious from the following description, or will be understood by the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is a structure schematic view of the heating incubation device of the embodiment of the present application;

[0018] Figure 2is a structure schematic view of the heating incubation device in another direction of the embodiment of the utility model;

[0019] Figure 3 is a structure schematic view of the cell culture container placed in the heating incubation device of the embodiment of the utility model;

[0020] Figure 4 is a structure schematic view of the heating assembly of the heating incubation device of the embodiment of the utility model;

[0021] Figure 5 is the exploded structure schematic view of the heating assembly of the heating incubation device of the embodiment of the utility model.

[0022] Reference signs:

[0023] 100, shell;110, wire hole;

[0024] 200, heating assembly;210, heat insulation piece;211, mounting groove;212, first mounting hole;220, heating piece;221, second mounting hole;230, metal heat conduction piece;231, limiting portion;232, positioning groove;233, first assembly hole;234, second assembly hole;240, temperature sensor;250, temperature protection switch;

[0025] 300, support column;

[0026] 400, cell culture container. Specific embodiments

[0027] The utility model will be explained further in detail below in combination with the drawings and embodiments. It can be understood that the specific embodiments described here are only for explaining the utility model, and not for limiting the utility model. In addition, it should be noted that, for the convenience of description, only the parts related to the utility model are shown in the drawings, not all structures.

[0028] In the description of the utility model, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, can be fixedly connected, can be detachably connected, or integrated;Can be mechanically connected, or electrically connected;Can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0029] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0030] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0031] The following is a reference appendix. Figures 1 to 5 The specific structure of the heating incubation device of this utility model is described.

[0032] This utility model discloses a heating incubation device, referenced Figure 1 and Figure 5 As shown, the heating incubation device includes a housing 100 and a heating assembly 200. The housing 100 defines an installation cavity, and the heating assembly 200 is disposed within the installation cavity. The heating assembly 200 includes a heat insulation component 210, a heating component 220, and a metal heat-conducting component 230. The heating component 220 is mounted on the heat insulation component 210, and the metal heat-conducting component 230 is mounted on the heating component 220 and serves to support the cell culture container 400. It is understood that during actual operation, when the cell culture container 400 is placed on the metal heat-conducting component 230, the heat generated by the heating component 220 can be transferred to the cell culture container 400 through the metal heat-conducting component 230. This direct heating method using the metal heat-conducting component 230 to heat the cell culture container 400 reduces temperature fluctuations in the cell growth environment during digestion and the termination of digestion, thereby effectively improving the uniformity of liquid heating within the cell culture container 400 and enhancing the consistency of the liquid temperature field. Furthermore, due to the presence of the heat insulation component 210, less of the heat generated by the heating component 220 is transferred to the environment through the heat insulation component 210, which effectively reduces the heat loss of the heating component 220 and reduces the equipment's heating power requirements.

[0033] In view of the fact that the cell culture container 400 needs a clean environment, the cell culture container 400 can be provided with a ventilated biosafety cabinet, and the biosafety cabinet has circulating air when it is started or in a working state. Therefore, the heating incubation device of the embodiment is provided with the windproof shell 100, which is used to reduce the influence of the wind flowing in the biosafety cabinet on the incubation temperature.

[0034] It should be noted that the heating assembly 200 in the embodiment is four, and in other embodiments of the utility model, the assembly of the heating assembly 200 can be two, three or more than four, which can be selected according to actual needs.

[0035] Optionally, the shell 100 has a rear side plate, a left side plate, a right side plate, an upper side plate and a lower side plate, and the front side of the mounting cavity is open. Therefore, only one open end is left as an opening for putting in and taking out the cell culture container 400, and the remaining side faces are closed, which is conducive to improving the windproof effect of the shell 100. Of course, in other embodiments of the utility model, the structure of the shell 100 can also be designed according to actual needs, and the direction of the open end of the mounting cavity can also be adjusted according to actual needs, and is not limited to the above description.

[0036] Optionally, the heating element 220 can be a PI heating film, a ceramic heating sheet of a specific shape, a silica gel heating sheet, etc., and preferably a PI heating film with a suitable power can be selected, which can meet the heating requirements of a T75 cell culture bottle or a 96-well plate and other cell culture containers 400, such as a power range of 20W-100W.

[0037] Optionally, the metal heat-conducting element 230 can be made of aluminum, copper, stainless steel and other metals, or other materials with good heat-conducting properties can also be used according to actual needs.

[0038] Optionally, the material of the heat insulation element 210 can be POM, ABS, bakelite and other insulating materials with low thermal conductivity (less than 1W / (m·K)).

[0039] Reference Figure 5 As shown in the figure, the heating assembly 200 further comprises a temperature sensor 240 for detecting the temperature of the heating element 220. It can be understood that in the actual incubation process, too high or too low incubation temperature will affect the incubation effect, and the added temperature sensor 240 can realize real-time monitoring of the temperature of the heating element 220, so as to ensure that the cell culture container 400 is always at a suitable incubation temperature under the heating of the metal heat-conducting element 230, thereby improving the incubation effect.

[0040] Optionally, the temperature sensor 240 can be a PT1000, and is installed on the metal heat-conducting member 230 away from the surface in contact with the cell culture container 400. This arrangement can prolong the response time of the temperature sensor 240, and ensure that the temperature of the entire heating member 220 has good uniformity. Further optionally, the metal heat-conducting member 230 is provided with a first assembly hole 233, and the temperature sensor 240 is installed in the first assembly hole 233, thereby improving the detection accuracy of the temperature sensor 240. Of course, in other embodiments of the present application, the temperature sensor 240 can also select other signals according to actual needs, and is not limited to the above definition.

[0041] Reference Figure 5 As shown in the drawings, the heating assembly 200 further comprises a temperature protection switch 250, which is electrically connected with the heating member 220, and is used to disconnect the circuit of the heating member 220 when the temperature of the heating member 220 is too high. It can be understood that the added temperature protection switch 250 can avoid the phenomenon of overheating of the heating member 220. When the temperature sensor 240 detects that the temperature of the heating member 220 is too high, the temperature protection switch 250 can automatically disconnect the circuit of the heating member 220 to stop working. This not only helps to reduce the probability of damage caused by overheating of the heating member 220, but also helps to improve the working reliability of the heating incubation device. Optionally, the metal heat-conducting member 230 is provided with a second assembly hole 234, and the temperature protection switch 250 is installed in the second assembly hole 234, thereby protecting the temperature protection switch 250, so as to ensure that the temperature protection switch 250 can work reliably. In the embodiments of the present application, the model and specific working mode of the temperature protection switch 250 can be selected according to actual needs, and the related parameters of the temperature protection switch 250 are not limited herein.

[0042] It should be noted that, since each heating assembly 200 is provided with a temperature sensor 240 and a temperature protection switch 250, the multiple heating assemblies 200 of the heating incubation device of the present embodiment can be independently arranged and do not interfere with each other. Each heating assembly 200 can be controlled by a separate port to control the incubation temperature. The incubation temperature can be a temperature varying within 25-50℃, and further can be stabilized within 37℃±0.2℃ through a temperature control algorithm. In addition, the heating incubation device has a main control board for controlling the working state of the multiple heating assemblies 200. The main control board can be a centralized or distributed controller. For example, the controller can be a single microcontroller, or can be composed of multiple microcontrollers distributedly. The microcontroller can run a control program.

[0043] Reference Figure 4As shown, the metal heat-conducting piece 230 is provided with a plurality of limiting portions 231, and the plurality of limiting portions 231 are respectively abutted on different side walls of the cell culture container 400 to limit the cell culture container 400 on the metal heat-conducting piece 230. It can be understood that the cell culture container 400 is limited on the metal heat-conducting piece 230 by the plurality of limiting portions 231, which can ensure the stability of the cell culture container 400 in the heating incubation device and avoid the phenomenon that the cell culture container 400 shakes to affect the incubation effect. Optionally, the limiting portion 231 includes three limiting protrusions, and the three limiting protrusions are respectively abutted on the left side wall, the right side wall and the bottom wall of the cell culture container 400. Therefore, the limiting effect of the cell culture container 400 can be improved. Of course, in other embodiments of the present application, the number and specific arrangement of the limiting portion 231 can be adjusted according to actual needs, and are not limited to the above description.

[0044] Reference Figure 4 As shown, the metal heat-conducting piece 230 has a positioning groove 232, and the positioning groove 232 is matched with the outer contour of the cell culture container 400. It can be understood that the positioning groove 232 can provide a reference for the placement of the cell culture container 400, so that the cell culture container 400 can be accurately placed in the specified position, and the heating effect of the metal heat-conducting piece 230 on the cell culture container 400 can be ensured. In other embodiments of the present application, the shape of the metal heat-conducting piece 230 can also be matched with the cell culture container 400 to ensure stable heating of the cell culture container 400.

[0045] Reference Figure 4 As shown, the heat insulation piece 210 has a mounting groove 211, and the heating piece 220 and the metal heat-conducting piece 230 are both mounted in the mounting groove 211. It can be understood that the heat insulation piece 210 and the metal heat-conducting piece 230 are both mounted in the mounting groove 211 of the heat insulation piece 210, which is beneficial to improve the heat insulation effect of the heat insulation piece 210 and can effectively reduce the heat loss of the heating piece 220 and reduce the demand of the equipment on the heating power.

[0046] Optionally, the metal heat-conducting piece 230 is adhered to the heating piece 220 by heat-conducting glue. In this way, on the one hand, the connection between the metal heat-conducting piece 230 and the heating piece 220 can be facilitated, and on the other hand, the heat generated by the heating piece 220 can be quickly transmitted to the metal heat-conducting piece 230.

[0047] Optionally, the bottom wall of the mounting groove 211 is provided with a first mounting hole 212, the heating element 220 is provided with a second mounting hole 221, and the metal heat conduction element 230 is provided with a third mounting hole. The connecting element is sequentially inserted into the first mounting hole 212, the second mounting hole 221 and the third mounting hole to connect the heat insulation element 210, the heating element 220 and the metal heat conduction element 230. It can be understood that, in the actual connection process, the metal heat conduction element 230 is first bonded to the heating element 220 by using a heat-conducting adhesive, then the metal heat conduction element 230 and the heating element 220 are mounted into the mounting groove 211, and finally the connecting element is used to sequentially insert into the first mounting hole 212, the second mounting hole 221 and the third mounting hole to connect the heat insulation element 210, the heating element 220 and the metal heat conduction element 230. In this way, the connection of the heat insulation element 210, the heating element 220 and the metal heat conduction element 230 can be facilitated. In the embodiment of the utility model, the connecting element can be a screw, a pin or a rivet.

[0048] Optionally, the heating assembly 200 is multiple groups, and the multiple groups of heating assemblies 200 are arranged in the mounting cavity in a horizontal direction and are spaced apart, and each group of heating assemblies 200 comprises a plurality of heating assemblies 200 arranged in a vertical direction and spaced apart. It can be understood that the plurality of heating assemblies 200 form multiple rows and multiple columns, so that the heating incubation device of the embodiment can incubate multiple cell culture containers 400 at the same time, thereby improving the incubation efficiency.

[0049] Referring to FIGS. 1, 2 and 3, Figure 1 and Figure 3 As shown in the figures, each heating assembly 200 is provided with a support hole, and two heating assemblies 200 arranged adjacent to each other in a vertical direction in each group of heating assemblies 200 are provided with a support column 300, and the two ends of the support column 300 are respectively inserted into the support holes of the adjacent two heating assemblies 200. It can be understood that the support column 300 is used to fix the heating assembly 200 located above in the vertical direction, and multiple support plates do not need to be arranged inside the mounting cavity, which is beneficial to simplify the structure of the shell 100, and in the actual operation process, different support columns 300 can be selected according to the height of the cell culture container 400, so as to accommodate multiple cell culture containers 400 of different specifications, expand the application range of the heating incubation device, and improve the user's satisfaction.

[0050] Referring to FIGS. 1, 2 and 3, Figure 2 As shown in the figures, the shell 100 is also provided with a wire hole 110, the wire hole 110 is arranged corresponding to the heating assembly 200, and is used to lead out the cable of the heating assembly 200. It can be understood that the wire hole 110 is used for the cable of the heating assembly 200 to go out of line, so as to facilitate the connection of the heating assembly 200 and the external power supply, and a battery does not need to be arranged inside the mounting cavity, which is beneficial to expand the application range of the heating incubation device.

[0051] In the description of the specification, the description referring to the terms "some embodiments", "other embodiments", etc., means that the particular feature, structure, material, or characteristic being described is included in at least one embodiment or example of the application. The appearances of the above-described terms in various places in the specification are not necessarily all referring to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples.

[0052] Obviously, the above embodiments of the application are merely exemplary and are not intended to limit the embodiments of the application. For those skilled in the art, various obvious changes, modifications and replacements can be made without departing from the scope of the application. Here, it is not necessary and impossible to enumerate all the embodiments. Any modification, equivalent replacement and improvement made within the spirit and principle of the application shall be included in the protection scope of the claims of the application.

Claims

1. A heating incubation apparatus, characterized in that, include: A housing (100) defines a mounting cavity; At least one heating component (200) is disposed within the mounting cavity. The heating component (200) includes a heat insulation element (210), a heating element (220), and a metal heat-conducting element (230). The heating element (220) is mounted on the heat insulation element (210), and the metal heat-conducting element (230) is mounted on the heating element (220) and is used to support the cell culture container (400).

2. The heating incubation apparatus according to claim 1, characterized in that, The heating assembly (200) also includes a temperature sensor (240) for detecting the temperature of the heating element (220).

3. The heating incubation apparatus according to claim 1, characterized in that, The heating assembly (200) also includes a temperature protection switch (250), which is electrically connected to the heating element (220) and is used to disconnect the circuit of the heating element (220) when the temperature of the heating element (220) is too high.

4. The heating incubation apparatus according to claim 1, characterized in that, The metal heat-conducting component (230) is provided with a plurality of limiting parts (231), and the plurality of limiting parts (231) respectively abut against different side walls of the cell culture container (400) to limit the cell culture container (400) to the metal heat-conducting component (230).

5. The heating incubation apparatus according to claim 1, characterized in that, The metal heat-conducting component (230) has a positioning groove (232) that matches the outer contour of the cell culture container (400).

6. The heating incubation apparatus according to claim 1, characterized in that, The heat insulation component (210) has a mounting groove (211), and the heating component (220) and the metal heat-conducting component (230) are both installed in the mounting groove (211).

7. The heating incubation apparatus according to claim 6, characterized in that, The mounting groove (211) has a first mounting hole (212) on its bottom wall, the heating element (220) has a second mounting hole (221), and the metal heat-conducting element (230) has a third mounting hole; wherein: The connector passes through the first mounting hole (212) and the second mounting hole (221) in sequence and is inserted into the third mounting hole to connect the heat insulation component (210), the heating component (220) and the metal heat-conducting component (230).

8. The heating incubation apparatus according to any one of claims 1-7, characterized in that, The heating components (200) are in multiple groups, and the multiple groups of heating components (200) are spaced apart in the mounting cavity along the horizontal direction, and each group of heating components (200) includes multiple heating components (200) spaced apart in the vertical direction.

9. The heating incubation apparatus according to claim 8, characterized in that, Each of the heating components (200) is provided with a support hole. A support column (300) is provided between two adjacent heating components (200) arranged in the vertical direction in each group of heating components (200). The two ends of the support column (300) are respectively inserted into the support holes of the two adjacent heating components (200).

10. The heating incubation apparatus according to any one of claims 1-7, characterized in that, The housing (100) is also provided with a wire hole (110), which is correspondingly provided with the heating component (200) and is used to lead out the cable of the heating component (200).

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